AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0844400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0844400 MtrunA17_Chr5g0415341 96.959 296 9 0 1 296 1 296 0.0 591
Msa0844400 MtrunA17_Chr8g0381971 39.209 278 141 8 1 266 1 262 1.37e-55 181
Msa0844400 MtrunA17_Chr4g0069121 31.276 243 151 8 27 266 34 263 8.67e-26 103
Msa0844400 MtrunA17_Chr4g0058901 27.358 318 192 14 3 295 8 311 3.14e-24 99.8
Msa0844400 MtrunA17_Chr4g0075211 30.000 260 149 11 23 266 34 276 8.03e-21 90.9
Msa0844400 MtrunA17_Chr2g0288891 28.244 262 151 12 23 266 20 262 1.88e-18 84.0
Msa0844400 MtrunA17_Chr7g0271981 27.404 208 126 9 20 222 25 212 3.12e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0844400 AT1G10380.1 53.405 279 120 7 25 296 27 302 2.46e-97 289
Msa0844400 AT1G10380.2 58.233 249 96 6 25 268 27 272 1.01e-94 282
Msa0844400 AT3G17350.1 33.333 246 145 9 27 264 31 265 9.71e-25 101
Msa0844400 AT3G17350.2 33.333 246 145 9 27 264 31 265 9.83e-25 100
Msa0844400 AT5G50290.1 30.147 272 165 11 10 266 5 266 9.63e-23 95.9
Msa0844400 AT1G11915.1 26.421 299 181 12 10 288 14 293 4.89e-17 80.5

Find 72 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCCAAATTCCATAAAA+AGG 0.207271 5_4:+22835745 None:intergenic
GCATAGTCAATTGAGGTAAT+AGG 0.216590 5_4:+22836957 None:intergenic
ACAATCTACTAAAGCGAATA+TGG 0.279440 5_4:+22836824 None:intergenic
TTCACATCATCTTACAATAA+TGG 0.287235 5_4:-22836369 Msa0844400:CDS
GTTCTTTGCCACAATTTCTT+TGG 0.298152 5_4:+22837090 None:intergenic
GAAGCGAGGGTCGCCGCAAC+CGG 0.304646 5_4:+22837040 None:intergenic
GTGCTATCGTCGAAAGTAAA+AGG 0.333825 5_4:+22836846 None:intergenic
CTTCAATGCAGTTCATATAC+AGG 0.342561 5_4:-22836597 Msa0844400:CDS
AATAGGACACAAGAAATAAA+TGG 0.343228 5_4:+22835866 None:intergenic
CATAGTCAATTGAGGTAATA+GGG 0.351341 5_4:+22836958 None:intergenic
GTGATGTTATGTATTCTTGT+AGG 0.354020 5_4:-22836713 Msa0844400:CDS
AGGAAGTAGGAAAAGGAAAA+TGG 0.359989 5_4:+22837136 None:intergenic
TTCTTTGGCACATTTGAGAT+TGG 0.364687 5_4:+22837105 None:intergenic
GATCCTCAAAAGTGGAACTA+TGG 0.374420 5_4:-22836546 Msa0844400:CDS
TTCGGTAGCGGCCCCGGTTG+CGG 0.383447 5_4:-22837053 Msa0844400:CDS
GCAAAGGCTTCGGCTTAGAT+TGG 0.390000 5_4:-22836875 Msa0844400:CDS
AGGTTAATATTAGTACTAAT+AGG 0.427315 5_4:+22836690 None:intergenic
TACCCTTTCGGTAGCGGCCC+CGG 0.428105 5_4:-22837059 Msa0844400:CDS
GTTTATACACCGGTTAATCT+TGG 0.430829 5_4:-22836648 Msa0844400:CDS
TAAACACAACATGTCGAAAT+CGG 0.436820 5_4:+22836666 None:intergenic
GCTGCATGTGAAAGGAGCTT+TGG 0.444173 5_4:-22836471 Msa0844400:CDS
TTATTTCTTGTGTCCTATTG+TGG 0.446874 5_4:-22835862 Msa0844400:three_prime_UTR
AAGCGAGGGTCGCCGCAACC+GGG 0.448647 5_4:+22837041 None:intergenic
CTCATCTTATAAGCAATTGA+TGG 0.460174 5_4:-22836006 Msa0844400:exon
AACAAATTGATCCTCAAAAG+TGG 0.460285 5_4:-22836554 Msa0844400:CDS
GTAGTGGTGTTAATTCCATT+AGG 0.460938 5_4:+22836402 None:intergenic
GTGAAAACCTTTACAATCCT+AGG 0.472272 5_4:+22835962 None:intergenic
GAACCTCTCAAGTACCCTTT+CGG 0.476764 5_4:-22837071 Msa0844400:CDS
AAAGCTGGACCAAGATTAAC+CGG 0.476906 5_4:+22836639 None:intergenic
TCAAAGAAGGAAGTAGGAAA+AGG 0.481402 5_4:+22837129 None:intergenic
TTGATTTGCATAGTCAATTG+AGG 0.482998 5_4:+22836950 None:intergenic
ATTGGATCAAAGAAGGAAGT+AGG 0.483664 5_4:+22837123 None:intergenic
ATGGTGAGAAGAGGAAGTGA+AGG 0.485023 5_4:+22837155 None:intergenic
TGAGAGAATGAGGGAGGACA+AGG 0.497901 5_4:+22837201 None:intergenic
ATTTGAGATTGGATCAAAGA+AGG 0.498634 5_4:+22837116 None:intergenic
TGAAGATCCATATCAAAAGC+TGG 0.499024 5_4:+22836624 None:intergenic
ACCCTACCTAGCAAAGGCTT+CGG 0.503040 5_4:-22836885 Msa0844400:CDS
TGTTGGCTGCAAGTTATATG+TGG 0.504485 5_4:+22837014 None:intergenic
ATGATCACCTAGGATTGTAA+AGG 0.507640 5_4:-22835969 Msa0844400:three_prime_UTR
ATGAGGGAGGACAAGGAATG+AGG 0.515161 5_4:+22837208 None:intergenic
AACCGGGGCCGCTACCGAAA+GGG 0.520766 5_4:+22837057 None:intergenic
CTCAAGTACCCTTTCGGTAG+CGG 0.533088 5_4:-22837065 Msa0844400:CDS
CAACCGGGGCCGCTACCGAA+AGG 0.537917 5_4:+22837056 None:intergenic
GCCGAAGCCTTTGCTAGGTA+GGG 0.540178 5_4:+22836884 None:intergenic
AAATCAAGAAGTTATGATGA+TGG 0.541719 5_4:-22836777 Msa0844400:CDS
TGTGATTGAGAGAATGAGGG+AGG 0.542861 5_4:+22837195 None:intergenic
AAAAGGAAAATGGTGAGAAG+AGG 0.550845 5_4:+22837146 None:intergenic
CAAATGTGCCAAAGAAATTG+TGG 0.553792 5_4:-22837098 Msa0844400:CDS
TTATATGTGGTTGGAAGCGA+GGG 0.559135 5_4:+22837027 None:intergenic
TCACATGCAGCACAAGAGCT+AGG 0.560587 5_4:+22836483 None:intergenic
AGCGAGGGTCGCCGCAACCG+GGG 0.560831 5_4:+22837042 None:intergenic
GACATGTTGTGTTTATACAC+CGG 0.561125 5_4:-22836658 Msa0844400:CDS
GCTCTTGTGCTGCATGTGAA+AGG 0.562347 5_4:-22836479 Msa0844400:CDS
GGCTGCAAGTTATATGTGGT+TGG 0.563811 5_4:+22837018 None:intergenic
GTTATATGTGGTTGGAAGCG+AGG 0.565909 5_4:+22837026 None:intergenic
GGAGCCTGTGTGTGTTGTGA+AGG 0.568111 5_4:+22836983 None:intergenic
AGCCGAAGCCTTTGCTAGGT+AGG 0.568732 5_4:+22836883 None:intergenic
TCTAAGCCGAAGCCTTTGCT+AGG 0.577620 5_4:+22836879 None:intergenic
TGGTGAGAAGAGGAAGTGAA+GGG 0.589263 5_4:+22837156 None:intergenic
CAAGAACATGTTGACATGGT+GGG 0.593775 5_4:+22836909 None:intergenic
CACAACATGTCGAAATCGGA+AGG 0.597606 5_4:+22836670 None:intergenic
CTCACCTTCACAACACACAC+AGG 0.609375 5_4:-22836987 Msa0844400:CDS
GTCAATTGAGGTAATAGGGT+AGG 0.619560 5_4:+22836962 None:intergenic
CTACCGAAAGGGTACTTGAG+AGG 0.634715 5_4:+22837068 None:intergenic
TCTTGTACCCTACCTAGCAA+AGG 0.635014 5_4:-22836891 Msa0844400:CDS
AATCAAGCTTATGATCACCT+AGG 0.658302 5_4:-22835979 Msa0844400:three_prime_UTR
ACAAGAACATGTTGACATGG+TGG 0.661350 5_4:+22836908 None:intergenic
GAATTTGGAATGGACTGACT+AGG 0.665769 5_4:-22835735 Msa0844400:three_prime_UTR
ATCATAATGATAATGAAACA+AGG 0.679852 5_4:+22835698 None:intergenic
GGTACAAGAACATGTTGACA+TGG 0.681333 5_4:+22836905 None:intergenic
TGTGAAGAAACAATCTGTAG+TGG 0.691455 5_4:+22836386 None:intergenic
TTTGGAATGGACTGACTAGG+TGG 0.705580 5_4:-22835732 Msa0844400:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTTTAAAATTATTAT+CGG - chr5_4:22836752-22836771 Msa0844400:CDS 0.0%
!!! TAATATTTTAAAATTATTAT+CGG - chr5_4:22836752-22836771 Msa0844400:CDS 0.0%
!!! TAAGATTATTTTTTTTGAAA+AGG - chr5_4:22836645-22836664 Msa0844400:CDS 10.0%
!!! TAAGATTATTTTTTTTGAAA+AGG - chr5_4:22836645-22836664 Msa0844400:CDS 10.0%
!! TAAAAATGAATGAAAAAAGT+AGG + chr5_4:22836821-22836840 None:intergenic 15.0%
!! TAAAAATGAATGAAAAAAGT+AGG + chr5_4:22836821-22836840 None:intergenic 15.0%
!! AGGTTAATATTAGTACTAAT+AGG + chr5_4:22836233-22836252 None:intergenic 20.0%
!!! TACAATAATGGTTTTAGAAA+TGG - chr5_4:22836563-22836582 Msa0844400:CDS 20.0%
!!! ACACTAAAATTTAAGATTAG+AGG - chr5_4:22836669-22836688 Msa0844400:CDS 20.0%
!!! CACTAAAATTTAAGATTAGA+GGG - chr5_4:22836670-22836689 Msa0844400:CDS 20.0%
!!! TTAAGTGTATGGAATTTTAA+TGG - chr5_4:22836984-22837003 Msa0844400:CDS 20.0%
!!! AAAAAGGGTTTTTTGTTTTT+GGG + chr5_4:22837162-22837181 None:intergenic 20.0%
!!! TAAAAAGGGTTTTTTGTTTT+TGG + chr5_4:22837163-22837182 None:intergenic 20.0%
!!! AAAACAAAAAACCCTTTTTA+TGG - chr5_4:22837163-22837182 Msa0844400:CDS 20.0%
!!! ATCATAATGATAATGAAACA+AGG + chr5_4:22837225-22837244 None:intergenic 20.0%
!! AGGTTAATATTAGTACTAAT+AGG + chr5_4:22836233-22836252 None:intergenic 20.0%
!!! TACAATAATGGTTTTAGAAA+TGG - chr5_4:22836563-22836582 Msa0844400:CDS 20.0%
!!! ACACTAAAATTTAAGATTAG+AGG - chr5_4:22836669-22836688 Msa0844400:CDS 20.0%
!!! CACTAAAATTTAAGATTAGA+GGG - chr5_4:22836670-22836689 Msa0844400:CDS 20.0%
!!! TTAAGTGTATGGAATTTTAA+TGG - chr5_4:22836984-22837003 Msa0844400:CDS 20.0%
!!! AAAAAGGGTTTTTTGTTTTT+GGG + chr5_4:22837162-22837181 None:intergenic 20.0%
!!! TAAAAAGGGTTTTTTGTTTT+TGG + chr5_4:22837163-22837182 None:intergenic 20.0%
!!! AAAACAAAAAACCCTTTTTA+TGG - chr5_4:22837163-22837182 Msa0844400:CDS 20.0%
!!! ATCATAATGATAATGAAACA+AGG + chr5_4:22837225-22837244 None:intergenic 20.0%
! AAATCAAGAAGTTATGATGA+TGG - chr5_4:22836143-22836162 Msa0844400:intron 25.0%
! TTCACATCATCTTACAATAA+TGG - chr5_4:22836551-22836570 Msa0844400:CDS 25.0%
! TGCAATTGACAAATAAGAAA+GGG + chr5_4:22836603-22836622 None:intergenic 25.0%
! ATGCAATTGACAAATAAGAA+AGG + chr5_4:22836604-22836623 None:intergenic 25.0%
!!! TCATTTTTAATGACTTGTTC+TGG - chr5_4:22836832-22836851 Msa0844400:CDS 25.0%
!!! GTTTTCACATTTTAAGTGTA+TGG - chr5_4:22836973-22836992 Msa0844400:CDS 25.0%
! AATAGGACACAAGAAATAAA+TGG + chr5_4:22837057-22837076 None:intergenic 25.0%
!!! AAAAGGGTTTTTTGTTTTTG+GGG + chr5_4:22837161-22837180 None:intergenic 25.0%
!! AAAACCCTTTTTATGGAATT+TGG - chr5_4:22837170-22837189 Msa0844400:CDS 25.0%
! CATTCCAAATTCCATAAAAA+GGG + chr5_4:22837177-22837196 None:intergenic 25.0%
! AAATCAAGAAGTTATGATGA+TGG - chr5_4:22836143-22836162 Msa0844400:intron 25.0%
! TTCACATCATCTTACAATAA+TGG - chr5_4:22836551-22836570 Msa0844400:CDS 25.0%
! TGCAATTGACAAATAAGAAA+GGG + chr5_4:22836603-22836622 None:intergenic 25.0%
! ATGCAATTGACAAATAAGAA+AGG + chr5_4:22836604-22836623 None:intergenic 25.0%
!!! TCATTTTTAATGACTTGTTC+TGG - chr5_4:22836832-22836851 Msa0844400:CDS 25.0%
!!! GTTTTCACATTTTAAGTGTA+TGG - chr5_4:22836973-22836992 Msa0844400:CDS 25.0%
! AATAGGACACAAGAAATAAA+TGG + chr5_4:22837057-22837076 None:intergenic 25.0%
!!! AAAAGGGTTTTTTGTTTTTG+GGG + chr5_4:22837161-22837180 None:intergenic 25.0%
!! AAAACCCTTTTTATGGAATT+TGG - chr5_4:22837170-22837189 Msa0844400:CDS 25.0%
! CATTCCAAATTCCATAAAAA+GGG + chr5_4:22837177-22837196 None:intergenic 25.0%
! TTTTGTGATTGAGAGAATGA+GGG + chr5_4:22835731-22835750 None:intergenic 30.0%
! ATTTTGTGATTGAGAGAATG+AGG + chr5_4:22835732-22835751 None:intergenic 30.0%
! ATTTGAGATTGGATCAAAGA+AGG + chr5_4:22835807-22835826 None:intergenic 30.0%
CATAGTCAATTGAGGTAATA+GGG + chr5_4:22835965-22835984 None:intergenic 30.0%
!! TTGATTTGCATAGTCAATTG+AGG + chr5_4:22835973-22835992 None:intergenic 30.0%
ACAATCTACTAAAGCGAATA+TGG + chr5_4:22836099-22836118 None:intergenic 30.0%
!! ATTTTGATCACATTGAAGCT+TGG + chr5_4:22836177-22836196 None:intergenic 30.0%
GTGATGTTATGTATTCTTGT+AGG - chr5_4:22836207-22836226 Msa0844400:intron 30.0%
TAAACACAACATGTCGAAAT+CGG + chr5_4:22836257-22836276 None:intergenic 30.0%
AACAAATTGATCCTCAAAAG+TGG - chr5_4:22836366-22836385 Msa0844400:CDS 30.0%
! TTTTTATGCAACTGTCCTAA+TGG - chr5_4:22836503-22836522 Msa0844400:CDS 30.0%
ATTGAATGTAACAACTGTGT+TGG + chr5_4:22836732-22836751 None:intergenic 30.0%
! TATAGAGTACTACACAAACA+CGG + chr5_4:22836793-22836812 None:intergenic 30.0%
CTCATCTTATAAGCAATTGA+TGG - chr5_4:22836914-22836933 Msa0844400:CDS 30.0%
!!! TGGAATTTTAATGGATTGCA+TGG - chr5_4:22836993-22837012 Msa0844400:CDS 30.0%
!!! GGAATTTTAATGGATTGCAT+GGG - chr5_4:22836994-22837013 Msa0844400:CDS 30.0%
!!! GAATTTTAATGGATTGCATG+GGG - chr5_4:22836995-22837014 Msa0844400:CDS 30.0%
!!! AATTTTAATGGATTGCATGG+GGG - chr5_4:22836996-22837015 Msa0844400:CDS 30.0%
! TTATTTCTTGTGTCCTATTG+TGG - chr5_4:22837058-22837077 Msa0844400:CDS 30.0%
CCATTCCAAATTCCATAAAA+AGG + chr5_4:22837178-22837197 None:intergenic 30.0%
! CCTTTTTATGGAATTTGGAA+TGG - chr5_4:22837175-22837194 Msa0844400:exon 30.0%
! TTTTGTGATTGAGAGAATGA+GGG + chr5_4:22835731-22835750 None:intergenic 30.0%
! ATTTTGTGATTGAGAGAATG+AGG + chr5_4:22835732-22835751 None:intergenic 30.0%
! ATTTGAGATTGGATCAAAGA+AGG + chr5_4:22835807-22835826 None:intergenic 30.0%
CATAGTCAATTGAGGTAATA+GGG + chr5_4:22835965-22835984 None:intergenic 30.0%
!! TTGATTTGCATAGTCAATTG+AGG + chr5_4:22835973-22835992 None:intergenic 30.0%
ACAATCTACTAAAGCGAATA+TGG + chr5_4:22836099-22836118 None:intergenic 30.0%
!! ATTTTGATCACATTGAAGCT+TGG + chr5_4:22836177-22836196 None:intergenic 30.0%
GTGATGTTATGTATTCTTGT+AGG - chr5_4:22836207-22836226 Msa0844400:intron 30.0%
TAAACACAACATGTCGAAAT+CGG + chr5_4:22836257-22836276 None:intergenic 30.0%
AACAAATTGATCCTCAAAAG+TGG - chr5_4:22836366-22836385 Msa0844400:CDS 30.0%
! TTTTTATGCAACTGTCCTAA+TGG - chr5_4:22836503-22836522 Msa0844400:CDS 30.0%
ATTGAATGTAACAACTGTGT+TGG + chr5_4:22836732-22836751 None:intergenic 30.0%
! TATAGAGTACTACACAAACA+CGG + chr5_4:22836793-22836812 None:intergenic 30.0%
CTCATCTTATAAGCAATTGA+TGG - chr5_4:22836914-22836933 Msa0844400:CDS 30.0%
!!! TGGAATTTTAATGGATTGCA+TGG - chr5_4:22836993-22837012 Msa0844400:CDS 30.0%
!!! GGAATTTTAATGGATTGCAT+GGG - chr5_4:22836994-22837013 Msa0844400:CDS 30.0%
!!! GAATTTTAATGGATTGCATG+GGG - chr5_4:22836995-22837014 Msa0844400:CDS 30.0%
!!! AATTTTAATGGATTGCATGG+GGG - chr5_4:22836996-22837015 Msa0844400:CDS 30.0%
! TTATTTCTTGTGTCCTATTG+TGG - chr5_4:22837058-22837077 Msa0844400:CDS 30.0%
CCATTCCAAATTCCATAAAA+AGG + chr5_4:22837178-22837197 None:intergenic 30.0%
! CCTTTTTATGGAATTTGGAA+TGG - chr5_4:22837175-22837194 Msa0844400:exon 30.0%
AAAAGGAAAATGGTGAGAAG+AGG + chr5_4:22835777-22835796 None:intergenic 35.0%
AGGAAGTAGGAAAAGGAAAA+TGG + chr5_4:22835787-22835806 None:intergenic 35.0%
TCAAAGAAGGAAGTAGGAAA+AGG + chr5_4:22835794-22835813 None:intergenic 35.0%
ATTGGATCAAAGAAGGAAGT+AGG + chr5_4:22835800-22835819 None:intergenic 35.0%
! TTCTTTGGCACATTTGAGAT+TGG + chr5_4:22835818-22835837 None:intergenic 35.0%
CAAATGTGCCAAAGAAATTG+TGG - chr5_4:22835822-22835841 Msa0844400:three_prime_UTR 35.0%
GTTCTTTGCCACAATTTCTT+TGG + chr5_4:22835833-22835852 None:intergenic 35.0%
! TTGTGAAGGTGAGTTTTTGT+TGG + chr5_4:22835926-22835945 None:intergenic 35.0%
GCATAGTCAATTGAGGTAAT+AGG + chr5_4:22835966-22835985 None:intergenic 35.0%
GACATGTTGTGTTTATACAC+CGG - chr5_4:22836262-22836281 Msa0844400:intron 35.0%
! GTTTATACACCGGTTAATCT+TGG - chr5_4:22836272-22836291 Msa0844400:intron 35.0%
TGAAGATCCATATCAAAAGC+TGG + chr5_4:22836299-22836318 None:intergenic 35.0%
CTTCAATGCAGTTCATATAC+AGG - chr5_4:22836323-22836342 Msa0844400:intron 35.0%
!! ATTCCATAGTTCCACTTTTG+AGG + chr5_4:22836380-22836399 None:intergenic 35.0%
! GTAGTGGTGTTAATTCCATT+AGG + chr5_4:22836521-22836540 None:intergenic 35.0%
TGTGAAGAAACAATCTGTAG+TGG + chr5_4:22836537-22836556 None:intergenic 35.0%
!! ATTATTATCGGTGTTGTGTC+AGG - chr5_4:22836764-22836783 Msa0844400:CDS 35.0%
! GGATGATATATGCTTTTGCA+TGG - chr5_4:22836879-22836898 Msa0844400:CDS 35.0%
! CTTTTGCATGGTCTTTGATT+AGG - chr5_4:22836891-22836910 Msa0844400:CDS 35.0%
AATCAAGCTTATGATCACCT+AGG - chr5_4:22836941-22836960 Msa0844400:CDS 35.0%
ATGATCACCTAGGATTGTAA+AGG - chr5_4:22836951-22836970 Msa0844400:CDS 35.0%
GTGAAAACCTTTACAATCCT+AGG + chr5_4:22836961-22836980 None:intergenic 35.0%
AAAAGGAAAATGGTGAGAAG+AGG + chr5_4:22835777-22835796 None:intergenic 35.0%
AGGAAGTAGGAAAAGGAAAA+TGG + chr5_4:22835787-22835806 None:intergenic 35.0%
TCAAAGAAGGAAGTAGGAAA+AGG + chr5_4:22835794-22835813 None:intergenic 35.0%
ATTGGATCAAAGAAGGAAGT+AGG + chr5_4:22835800-22835819 None:intergenic 35.0%
! TTCTTTGGCACATTTGAGAT+TGG + chr5_4:22835818-22835837 None:intergenic 35.0%
CAAATGTGCCAAAGAAATTG+TGG - chr5_4:22835822-22835841 Msa0844400:three_prime_UTR 35.0%
GTTCTTTGCCACAATTTCTT+TGG + chr5_4:22835833-22835852 None:intergenic 35.0%
! TTGTGAAGGTGAGTTTTTGT+TGG + chr5_4:22835926-22835945 None:intergenic 35.0%
GCATAGTCAATTGAGGTAAT+AGG + chr5_4:22835966-22835985 None:intergenic 35.0%
GACATGTTGTGTTTATACAC+CGG - chr5_4:22836262-22836281 Msa0844400:intron 35.0%
! GTTTATACACCGGTTAATCT+TGG - chr5_4:22836272-22836291 Msa0844400:intron 35.0%
TGAAGATCCATATCAAAAGC+TGG + chr5_4:22836299-22836318 None:intergenic 35.0%
CTTCAATGCAGTTCATATAC+AGG - chr5_4:22836323-22836342 Msa0844400:intron 35.0%
!! ATTCCATAGTTCCACTTTTG+AGG + chr5_4:22836380-22836399 None:intergenic 35.0%
! GTAGTGGTGTTAATTCCATT+AGG + chr5_4:22836521-22836540 None:intergenic 35.0%
TGTGAAGAAACAATCTGTAG+TGG + chr5_4:22836537-22836556 None:intergenic 35.0%
!! ATTATTATCGGTGTTGTGTC+AGG - chr5_4:22836764-22836783 Msa0844400:CDS 35.0%
! GGATGATATATGCTTTTGCA+TGG - chr5_4:22836879-22836898 Msa0844400:CDS 35.0%
! CTTTTGCATGGTCTTTGATT+AGG - chr5_4:22836891-22836910 Msa0844400:CDS 35.0%
AATCAAGCTTATGATCACCT+AGG - chr5_4:22836941-22836960 Msa0844400:CDS 35.0%
ATGATCACCTAGGATTGTAA+AGG - chr5_4:22836951-22836970 Msa0844400:CDS 35.0%
GTGAAAACCTTTACAATCCT+AGG + chr5_4:22836961-22836980 None:intergenic 35.0%
TTATATGTGGTTGGAAGCGA+GGG + chr5_4:22835896-22835915 None:intergenic 40.0%
! TGTTGGCTGCAAGTTATATG+TGG + chr5_4:22835909-22835928 None:intergenic 40.0%
GTCAATTGAGGTAATAGGGT+AGG + chr5_4:22835961-22835980 None:intergenic 40.0%
CAAGAACATGTTGACATGGT+GGG + chr5_4:22836014-22836033 None:intergenic 40.0%
ACAAGAACATGTTGACATGG+TGG + chr5_4:22836015-22836034 None:intergenic 40.0%
GGTACAAGAACATGTTGACA+TGG + chr5_4:22836018-22836037 None:intergenic 40.0%
GTGCTATCGTCGAAAGTAAA+AGG + chr5_4:22836077-22836096 None:intergenic 40.0%
AAAGCTGGACCAAGATTAAC+CGG + chr5_4:22836284-22836303 None:intergenic 40.0%
! TCTTGGTCCAGCTTTTGATA+TGG - chr5_4:22836289-22836308 Msa0844400:intron 40.0%
GATCCTCAAAAGTGGAACTA+TGG - chr5_4:22836374-22836393 Msa0844400:CDS 40.0%
!!! GAAAGGAGCTTTGGATTTTG+TGG - chr5_4:22836458-22836477 Msa0844400:CDS 40.0%
!! TTCTGGCCTTTCTGTTTTCA+GGG - chr5_4:22836849-22836868 Msa0844400:CDS 40.0%
AGCAAACCCTGAAAACAGAA+AGG + chr5_4:22836858-22836877 None:intergenic 40.0%
!! TTCTGTTTTCAGGGTTTGCT+TGG - chr5_4:22836858-22836877 Msa0844400:CDS 40.0%
! GTGCACACATTTTCCACAAT+AGG + chr5_4:22837074-22837093 None:intergenic 40.0%
!! GAATTTGGAATGGACTGACT+AGG - chr5_4:22837185-22837204 Msa0844400:exon 40.0%
TTATATGTGGTTGGAAGCGA+GGG + chr5_4:22835896-22835915 None:intergenic 40.0%
! TGTTGGCTGCAAGTTATATG+TGG + chr5_4:22835909-22835928 None:intergenic 40.0%
GTCAATTGAGGTAATAGGGT+AGG + chr5_4:22835961-22835980 None:intergenic 40.0%
CAAGAACATGTTGACATGGT+GGG + chr5_4:22836014-22836033 None:intergenic 40.0%
ACAAGAACATGTTGACATGG+TGG + chr5_4:22836015-22836034 None:intergenic 40.0%
GGTACAAGAACATGTTGACA+TGG + chr5_4:22836018-22836037 None:intergenic 40.0%
GTGCTATCGTCGAAAGTAAA+AGG + chr5_4:22836077-22836096 None:intergenic 40.0%
AAAGCTGGACCAAGATTAAC+CGG + chr5_4:22836284-22836303 None:intergenic 40.0%
! TCTTGGTCCAGCTTTTGATA+TGG - chr5_4:22836289-22836308 Msa0844400:intron 40.0%
GATCCTCAAAAGTGGAACTA+TGG - chr5_4:22836374-22836393 Msa0844400:CDS 40.0%
!!! GAAAGGAGCTTTGGATTTTG+TGG - chr5_4:22836458-22836477 Msa0844400:CDS 40.0%
!! TTCTGGCCTTTCTGTTTTCA+GGG - chr5_4:22836849-22836868 Msa0844400:CDS 40.0%
AGCAAACCCTGAAAACAGAA+AGG + chr5_4:22836858-22836877 None:intergenic 40.0%
!! TTCTGTTTTCAGGGTTTGCT+TGG - chr5_4:22836858-22836877 Msa0844400:CDS 40.0%
! GTGCACACATTTTCCACAAT+AGG + chr5_4:22837074-22837093 None:intergenic 40.0%
!! GAATTTGGAATGGACTGACT+AGG - chr5_4:22837185-22837204 Msa0844400:exon 40.0%
TGTGATTGAGAGAATGAGGG+AGG + chr5_4:22835728-22835747 None:intergenic 45.0%
TGGTGAGAAGAGGAAGTGAA+GGG + chr5_4:22835767-22835786 None:intergenic 45.0%
ATGGTGAGAAGAGGAAGTGA+AGG + chr5_4:22835768-22835787 None:intergenic 45.0%
GAACCTCTCAAGTACCCTTT+CGG - chr5_4:22835849-22835868 Msa0844400:three_prime_UTR 45.0%
GTTATATGTGGTTGGAAGCG+AGG + chr5_4:22835897-22835916 None:intergenic 45.0%
GGCTGCAAGTTATATGTGGT+TGG + chr5_4:22835905-22835924 None:intergenic 45.0%
TCTTGTACCCTACCTAGCAA+AGG - chr5_4:22836029-22836048 Msa0844400:CDS 45.0%
CACAACATGTCGAAATCGGA+AGG + chr5_4:22836253-22836272 None:intergenic 45.0%
!! GTTCTGGCCTTTCTGTTTTC+AGG - chr5_4:22836848-22836867 Msa0844400:CDS 45.0%
!! TTTGGAATGGACTGACTAGG+TGG - chr5_4:22837188-22837207 Msa0844400:exon 45.0%
TGTGATTGAGAGAATGAGGG+AGG + chr5_4:22835728-22835747 None:intergenic 45.0%
TGGTGAGAAGAGGAAGTGAA+GGG + chr5_4:22835767-22835786 None:intergenic 45.0%
ATGGTGAGAAGAGGAAGTGA+AGG + chr5_4:22835768-22835787 None:intergenic 45.0%
GAACCTCTCAAGTACCCTTT+CGG - chr5_4:22835849-22835868 Msa0844400:three_prime_UTR 45.0%
GTTATATGTGGTTGGAAGCG+AGG + chr5_4:22835897-22835916 None:intergenic 45.0%
GGCTGCAAGTTATATGTGGT+TGG + chr5_4:22835905-22835924 None:intergenic 45.0%
TCTTGTACCCTACCTAGCAA+AGG - chr5_4:22836029-22836048 Msa0844400:CDS 45.0%
CACAACATGTCGAAATCGGA+AGG + chr5_4:22836253-22836272 None:intergenic 45.0%
!! GTTCTGGCCTTTCTGTTTTC+AGG - chr5_4:22836848-22836867 Msa0844400:CDS 45.0%
!! TTTGGAATGGACTGACTAGG+TGG - chr5_4:22837188-22837207 Msa0844400:exon 45.0%
ATGAGGGAGGACAAGGAATG+AGG + chr5_4:22835715-22835734 None:intergenic 50.0%
TGAGAGAATGAGGGAGGACA+AGG + chr5_4:22835722-22835741 None:intergenic 50.0%
!! CTACCGAAAGGGTACTTGAG+AGG + chr5_4:22835855-22835874 None:intergenic 50.0%
CTCAAGTACCCTTTCGGTAG+CGG - chr5_4:22835855-22835874 Msa0844400:three_prime_UTR 50.0%
CTCACCTTCACAACACACAC+AGG - chr5_4:22835933-22835952 Msa0844400:three_prime_UTR 50.0%
ACCCTACCTAGCAAAGGCTT+CGG - chr5_4:22836035-22836054 Msa0844400:CDS 50.0%
TCTAAGCCGAAGCCTTTGCT+AGG + chr5_4:22836044-22836063 None:intergenic 50.0%
GCAAAGGCTTCGGCTTAGAT+TGG - chr5_4:22836045-22836064 Msa0844400:CDS 50.0%
TCACATGCAGCACAAGAGCT+AGG + chr5_4:22836440-22836459 None:intergenic 50.0%
GCTCTTGTGCTGCATGTGAA+AGG - chr5_4:22836441-22836460 Msa0844400:CDS 50.0%
GCTGCATGTGAAAGGAGCTT+TGG - chr5_4:22836449-22836468 Msa0844400:CDS 50.0%
ATGAGGGAGGACAAGGAATG+AGG + chr5_4:22835715-22835734 None:intergenic 50.0%
TGAGAGAATGAGGGAGGACA+AGG + chr5_4:22835722-22835741 None:intergenic 50.0%
!! CTACCGAAAGGGTACTTGAG+AGG + chr5_4:22835855-22835874 None:intergenic 50.0%
CTCAAGTACCCTTTCGGTAG+CGG - chr5_4:22835855-22835874 Msa0844400:three_prime_UTR 50.0%
CTCACCTTCACAACACACAC+AGG - chr5_4:22835933-22835952 Msa0844400:three_prime_UTR 50.0%
ACCCTACCTAGCAAAGGCTT+CGG - chr5_4:22836035-22836054 Msa0844400:CDS 50.0%
TCTAAGCCGAAGCCTTTGCT+AGG + chr5_4:22836044-22836063 None:intergenic 50.0%
GCAAAGGCTTCGGCTTAGAT+TGG - chr5_4:22836045-22836064 Msa0844400:CDS 50.0%
TCACATGCAGCACAAGAGCT+AGG + chr5_4:22836440-22836459 None:intergenic 50.0%
GCTCTTGTGCTGCATGTGAA+AGG - chr5_4:22836441-22836460 Msa0844400:CDS 50.0%
GCTGCATGTGAAAGGAGCTT+TGG - chr5_4:22836449-22836468 Msa0844400:CDS 50.0%
GGAGCCTGTGTGTGTTGTGA+AGG + chr5_4:22835940-22835959 None:intergenic 55.0%
GCCGAAGCCTTTGCTAGGTA+GGG + chr5_4:22836039-22836058 None:intergenic 55.0%
AGCCGAAGCCTTTGCTAGGT+AGG + chr5_4:22836040-22836059 None:intergenic 55.0%
GGAGCCTGTGTGTGTTGTGA+AGG + chr5_4:22835940-22835959 None:intergenic 55.0%
GCCGAAGCCTTTGCTAGGTA+GGG + chr5_4:22836039-22836058 None:intergenic 55.0%
AGCCGAAGCCTTTGCTAGGT+AGG + chr5_4:22836040-22836059 None:intergenic 55.0%
! TACCCTTTCGGTAGCGGCCC+CGG - chr5_4:22835861-22835880 Msa0844400:three_prime_UTR 65.0%
AACCGGGGCCGCTACCGAAA+GGG + chr5_4:22835866-22835885 None:intergenic 65.0%
! TACCCTTTCGGTAGCGGCCC+CGG - chr5_4:22835861-22835880 Msa0844400:three_prime_UTR 65.0%
AACCGGGGCCGCTACCGAAA+GGG + chr5_4:22835866-22835885 None:intergenic 65.0%
CAACCGGGGCCGCTACCGAA+AGG + chr5_4:22835867-22835886 None:intergenic 70.0%
! TTCGGTAGCGGCCCCGGTTG+CGG - chr5_4:22835867-22835886 Msa0844400:three_prime_UTR 70.0%
AAGCGAGGGTCGCCGCAACC+GGG + chr5_4:22835882-22835901 None:intergenic 70.0%
GAAGCGAGGGTCGCCGCAAC+CGG + chr5_4:22835883-22835902 None:intergenic 70.0%
CAACCGGGGCCGCTACCGAA+AGG + chr5_4:22835867-22835886 None:intergenic 70.0%
! TTCGGTAGCGGCCCCGGTTG+CGG - chr5_4:22835867-22835886 Msa0844400:three_prime_UTR 70.0%
AAGCGAGGGTCGCCGCAACC+GGG + chr5_4:22835882-22835901 None:intergenic 70.0%
GAAGCGAGGGTCGCCGCAAC+CGG + chr5_4:22835883-22835902 None:intergenic 70.0%
AGCGAGGGTCGCCGCAACCG+GGG + chr5_4:22835881-22835900 None:intergenic 75.0%
AGCGAGGGTCGCCGCAACCG+GGG + chr5_4:22835881-22835900 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 22835693 22837249 22835693 ID=Msa0844400;Name=Msa0844400
chr5_4 mRNA 22835693 22837249 22835693 ID=Msa0844400-mRNA-1;Parent=Msa0844400;Name=Msa0844400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=57|1|1|1|0|0|2|324|296
chr5_4 exon 22835693 22836072 22835693 ID=Msa0844400-mRNA-1:exon:9313;Parent=Msa0844400-mRNA-1
chr5_4 exon 22836358 22837249 22836358 ID=Msa0844400-mRNA-1:exon:9312;Parent=Msa0844400-mRNA-1
chr5_4 five_prime_UTR 22837193 22837249 22837193 ID=Msa0844400-mRNA-1:five_prime_utr;Parent=Msa0844400-mRNA-1
chr5_4 CDS 22836358 22837192 22836358 ID=Msa0844400-mRNA-1:cds;Parent=Msa0844400-mRNA-1
chr5_4 CDS 22836017 22836072 22836017 ID=Msa0844400-mRNA-1:cds;Parent=Msa0844400-mRNA-1
chr5_4 three_prime_UTR 22835693 22836016 22835693 ID=Msa0844400-mRNA-1:three_prime_utr;Parent=Msa0844400-mRNA-1
Gene Sequence

>Msa0844400

ATGAAATTTAGTTACCCTTCACTTCCTCTTCTCACCATTTTCCTTTTCCTACTTCCTTCTTTGATCCAATCTCAAATGTGCCAAAGAAATTGTGGCAAAGAACCTCTCAAGTACCCTTTCGGTAGCGGCCCCGGTTGCGGCGACCCTCGCTTCCAACCACATATAACTTGCAGCCAACAAAAACTCACCTTCACAACACACACAGGCTCCTACCCTATTACCTCAATTGACTATGCAAATCAAGTCATATACATTTCAGATCCCACCATGTCAACATGTTCTTGTACCCTACCTAGCAAAGGCTTCGGCTTAGATTGGAACGCGCCTTTTACTTTCGACGATAGCACCATATTCGCTTTAGTAGATTGTTCGATGAACTCATCATCGATATGTAAATCAAGAAGTTATGATGATGGAAGCAATTCCAAGCTTCAATGTGATCAAAATACACAAATTTGTGATGTTATGTATTCTTGTAGGCCTATTAGTACTAATATTAACCTTCCGATTTCGACATGTTGTGTTTATACACCGGTTAATCTTGGTCCAGCTTTTGATATGGATCTTCAAAAGCTTCAATGCAGTTCATATACAGGTTTTTACAATTACAATGATCAACAAATTGATCCTCAAAAGTGGAACTATGGAATTGCACTTAAGTATAAGTTTAGTGTTACAAATGATTATCCTAGCTCTTGTGCTGCATGTGAAAGGAGCTTTGGATTTTGTGGATATAGTGAAGTTTATAATTCATTTTTATGCAACTGTCCTAATGGAATTAACACCACTACAGATTGTTTCTTCACATCATCTTACAATAATGGTTTTAGAAATGGGTTTGCTTGGATGATATATGCTTTTGCATGGTCTTTGATTAGGCTCATCTTATAA
Protein sequence

>Msa0844400

MKFSYPSLPLLTIFLFLLPSLIQSQMCQRNCGKEPLKYPFGSGPGCGDPRFQPHITCSQQKLTFTTHTGSYPITSIDYANQVIYISDPTMSTCSCTLPSKGFGLDWNAPFTFDDSTIFALVDCSMNSSSICKSRSYDDGSNSKLQCDQNTQICDVMYSCRPISTNINLPISTCCVYTPVNLGPAFDMDLQKLQCSSYTGFYNYNDQQIDPQKWNYGIALKYKFSVTNDYPSSCAACERSFGFCGYSEVYNSFLCNCPNGINTTTDCFFTSSYNNGFRNGFAWMIYAFAWSLIRLIL*