Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0729000 | XP_013446401.1 | 97.436 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 2.32e-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0729000 | I3SVT4 | 97.436 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 1.11e-15 | 75.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0729000 | Msa0887990 | 0.817110 | 3.909323e-52 | -8.615850e-47 |
| Msa0729000 | Msa1077870 | 0.806220 | 9.144141e-50 | -8.615850e-47 |
| Msa0729000 | Msa1084240 | 0.820303 | 7.364591e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0729000 | MtrunA17_Chr8g0373021 | 97.436 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 2.13e-19 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAGACCTCCGTGAATT+AGG | 0.191054 | 5_1:+35453311 | Msa0729000:CDS |
| AGAAAGAATGAGTTTAGAAA+TGG | 0.381364 | 5_1:+35453223 | None:intergenic |
| ATGAAGACCTCCGTGAATTA+GGG | 0.409924 | 5_1:+35453312 | Msa0729000:CDS |
| CTTCATCTATAATCAAAACT+TGG | 0.456334 | 5_1:-35453295 | None:intergenic |
| TCATCAGAAGGTGAAGAAGA+AGG | 0.548262 | 5_1:+35453254 | Msa0729000:CDS |
| AATGAGTTTAGAAATGGCAG+CGG | 0.627697 | 5_1:+35453229 | None:intergenic |
| GAAGAAGGAAAGCTAAGCGG+AGG | 0.641558 | 5_1:+35453269 | Msa0729000:CDS |
| GCAGTAGCTGACACTCTAGG+CGG | 0.655421 | 5_1:-35453342 | None:intergenic |
| ATGGCAGCGGAATCATCAGA+AGG | 0.656350 | 5_1:+35453242 | Msa0729000:CDS |
| GAAGAAGAAGGAAAGCTAAG+CGG | 0.716861 | 5_1:+35453266 | Msa0729000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTCATCTATAATCAAAACT+TGG | - | chr5_1:35453298-35453317 | None:intergenic | 25.0% |
| TCATCAGAAGGTGAAGAAGA+AGG | + | chr5_1:35453254-35453273 | Msa0729000:CDS | 40.0% | |
| GAAGAAGAAGGAAAGCTAAG+CGG | + | chr5_1:35453266-35453285 | Msa0729000:CDS | 40.0% | |
| ATGAAGACCTCCGTGAATTA+GGG | + | chr5_1:35453312-35453331 | Msa0729000:CDS | 40.0% | |
| !!! | CTTTTTTCCCTAATTCACGG+AGG | - | chr5_1:35453322-35453341 | None:intergenic | 40.0% |
| !!! | CGGCTTTTTTCCCTAATTCA+CGG | - | chr5_1:35453325-35453344 | None:intergenic | 40.0% |
| GATGAAGACCTCCGTGAATT+AGG | + | chr5_1:35453311-35453330 | Msa0729000:CDS | 45.0% | |
| ATGGCAGCGGAATCATCAGA+AGG | + | chr5_1:35453242-35453261 | Msa0729000:CDS | 50.0% | |
| GAAGAAGGAAAGCTAAGCGG+AGG | + | chr5_1:35453269-35453288 | Msa0729000:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 35453230 | 35453349 | 35453230 | ID=Msa0729000;Name=Msa0729000 |
| chr5_1 | mRNA | 35453230 | 35453349 | 35453230 | ID=Msa0729000-mRNA-1;Parent=Msa0729000;Name=Msa0729000-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|39 |
| chr5_1 | exon | 35453230 | 35453349 | 35453230 | ID=Msa0729000-mRNA-1:exon:10959;Parent=Msa0729000-mRNA-1 |
| chr5_1 | CDS | 35453230 | 35453349 | 35453230 | ID=Msa0729000-mRNA-1:cds;Parent=Msa0729000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
