Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004880 | Msa1084240 | 0.800908 | 1.155820e-48 | -8.615850e-47 |
| Msa0017510 | Msa1084240 | 0.802775 | 4.780366e-49 | -8.615850e-47 |
| Msa0112750 | Msa1084240 | 0.828601 | 8.209450e-55 | -8.615850e-47 |
| Msa0627410 | Msa1084240 | 0.801800 | 7.588304e-49 | -8.615850e-47 |
| Msa0639460 | Msa1084240 | 0.808041 | 3.764049e-50 | -8.615850e-47 |
| Msa0657970 | Msa1084240 | 0.824712 | 6.955637e-54 | -8.615850e-47 |
| Msa0678930 | Msa1084240 | 0.827943 | 1.183482e-54 | -8.615850e-47 |
| Msa0682360 | Msa1084240 | 0.803631 | 3.179786e-49 | -8.615850e-47 |
| Msa0729000 | Msa1084240 | 0.820303 | 7.364591e-53 | -8.615850e-47 |
| Msa0736000 | Msa1084240 | 0.808342 | 3.246633e-50 | -8.615850e-47 |
| Msa0741570 | Msa1084240 | 0.825972 | 3.501607e-54 | -8.615850e-47 |
| Msa0793640 | Msa1084240 | 0.809964 | 1.458803e-50 | -8.615850e-47 |
| Msa0157290 | Msa1084240 | 0.802784 | 4.760645e-49 | -8.615850e-47 |
| Msa0399300 | Msa1084240 | 0.806739 | 7.105635e-50 | -8.615850e-47 |
| Msa0406120 | Msa1084240 | 0.811231 | 7.763808e-51 | -8.615850e-47 |
| Msa0431500 | Msa1084240 | 0.811959 | 5.392270e-51 | -8.615850e-47 |
| Msa0432290 | Msa1084240 | 0.802501 | 5.444110e-49 | -8.615850e-47 |
| Msa0452620 | Msa1084240 | 0.802794 | 4.737413e-49 | -8.615850e-47 |
| Msa0456200 | Msa1084240 | 0.815551 | 8.726369e-52 | -8.615850e-47 |
| Msa0985950 | Msa1084240 | 0.815292 | 9.960273e-52 | -8.615850e-47 |
| Msa0999340 | Msa1084240 | 0.806622 | 7.522608e-50 | -8.615850e-47 |
| Msa0999350 | Msa1084240 | 0.802775 | 4.780088e-49 | -8.615850e-47 |
| Msa0999400 | Msa1084240 | 0.806063 | 9.866312e-50 | -8.615850e-47 |
| Msa1024240 | Msa1084240 | 0.814181 | 1.756176e-51 | -8.615850e-47 |
| Msa1077870 | Msa1084240 | 0.822242 | 2.629913e-53 | -8.615850e-47 |
| Msa1084240 | Msa1172620 | 0.802835 | 4.645928e-49 | -8.615850e-47 |
| Msa1084240 | Msa1187940 | 0.825154 | 5.471969e-54 | -8.615850e-47 |
| Msa1084240 | Msa1228190 | 0.801737 | 7.820288e-49 | -8.615850e-47 |
| Msa1084240 | Msa1283860 | 0.800112 | 1.679255e-48 | -8.615850e-47 |
| Msa1084240 | Msa1324780 | 0.803231 | 3.847154e-49 | -8.615850e-47 |
| Msa1084240 | Msa1384370 | 0.803282 | 3.755699e-49 | -8.615850e-47 |
| Msa1084240 | Msa1397610 | 0.801515 | 8.684403e-49 | -8.615850e-47 |
| Msa1084240 | Msa1438250 | 0.847921 | 8.515981e-60 | -8.615850e-47 |
| Msa0324150 | Msa1084240 | 0.811269 | 7.618804e-51 | -8.615850e-47 |
| Msa0326200 | Msa1084240 | 0.817339 | 3.471037e-52 | -8.615850e-47 |
| Msa0353190 | Msa1084240 | 0.815460 | 9.138936e-52 | -8.615850e-47 |
| Msa0353690 | Msa1084240 | 0.825152 | 5.478226e-54 | -8.615850e-47 |
| Msa0360900 | Msa1084240 | 0.815296 | 9.942256e-52 | -8.615850e-47 |
| Msa0512900 | Msa1084240 | 0.819786 | 9.672113e-53 | -8.615850e-47 |
| Msa0858840 | Msa1084240 | 0.808513 | 2.985676e-50 | -8.615850e-47 |
| Msa0880140 | Msa1084240 | 0.800029 | 1.745521e-48 | -8.615850e-47 |
| Msa0887990 | Msa1084240 | 0.865489 | 5.691097e-65 | -8.615850e-47 |
| Msa0909140 | Msa1084240 | 0.807853 | 4.125865e-50 | -8.615850e-47 |
| Msa0922720 | Msa1084240 | 0.815443 | 9.221041e-52 | -8.615850e-47 |
| Msa0954920 | Msa1084240 | 0.811397 | 7.146483e-51 | -8.615850e-47 |
| Msa0961090 | Msa1084240 | 0.815880 | 7.367552e-52 | -8.615850e-47 |
| Msa0965750 | Msa1084240 | 0.818040 | 2.411788e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATGGTTAGAGATATTT+TGG | 0.162449 | 7_2:-84515441 | Msa1084240:CDS |
| TTAGAGTGTTGGACACTTAT+AGG | 0.267617 | 7_2:-84515370 | Msa1084240:CDS |
| GTCCAACACTCTAACACTTA+TGG | 0.280996 | 7_2:+84515310 | None:intergenic |
| GTTAGAGTGTTGGACACTTT+TGG | 0.309460 | 7_2:-84515302 | Msa1084240:CDS |
| ATTGGATGATGCATTGTTAA+TGG | 0.329350 | 7_2:+84515472 | None:intergenic |
| GGACACTTATAGGAGTTCTT+TGG | 0.344595 | 7_2:+84515277 | None:intergenic |
| CAATGCATCATCCAATGATA+TGG | 0.355846 | 7_2:-84515465 | Msa1084240:CDS |
| GGACACTTATAGGAGTTCCT+TGG | 0.388483 | 7_2:-84515360 | Msa1084240:CDS |
| GACACTTATAGGAGTTCCTT+GGG | 0.402734 | 7_2:-84515359 | Msa1084240:CDS |
| TCCAACACTCTAACACTTAT+GGG | 0.402950 | 7_2:+84515311 | None:intergenic |
| AACCATTGCAGCCATATCAT+TGG | 0.424567 | 7_2:+84515454 | None:intergenic |
| AGCTATTGACGAAACATGTA+TGG | 0.481189 | 7_2:+84515418 | None:intergenic |
| ATCCAATGATATGGCTGCAA+TGG | 0.534314 | 7_2:-84515456 | Msa1084240:CDS |
| TCCCATAAGTGTTAGAGTGT+TGG | 0.542041 | 7_2:-84515312 | Msa1084240:CDS |
| CAGCACAAGAGTTAGAGTGT+TGG | 0.606158 | 7_2:-84515381 | Msa1084240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGGATGATGCATTGTTAA+TGG | + | chr7_2:84515305-84515324 | None:intergenic | 30.0% | |
| ! | TGCAATGGTTAGAGATATTT+TGG | - | chr7_2:84515333-84515352 | Msa1084240:CDS | 30.0% |
| ! | TTACAGCATTTTCAACTTTC+AGG | + | chr7_2:84515441-84515460 | None:intergenic | 30.0% |
| CAATGCATCATCCAATGATA+TGG | - | chr7_2:84515309-84515328 | Msa1084240:CDS | 35.0% | |
| AGCTATTGACGAAACATGTA+TGG | + | chr7_2:84515359-84515378 | None:intergenic | 35.0% | |
| !! | TTAGAGTGTTGGACACTTAT+AGG | - | chr7_2:84515404-84515423 | Msa1084240:CDS | 35.0% |
| TCCAACACTCTAACACTTAT+GGG | + | chr7_2:84515466-84515485 | None:intergenic | 35.0% | |
| ATCCAATGATATGGCTGCAA+TGG | - | chr7_2:84515318-84515337 | Msa1084240:CDS | 40.0% | |
| AACCATTGCAGCCATATCAT+TGG | + | chr7_2:84515323-84515342 | None:intergenic | 40.0% | |
| GACACTTATAGGAGTTCCTT+GGG | - | chr7_2:84515415-84515434 | Msa1084240:CDS | 40.0% | |
| ! | ATTTTCAACTTTCAGGCCCA+AGG | + | chr7_2:84515434-84515453 | None:intergenic | 40.0% |
| TCCCATAAGTGTTAGAGTGT+TGG | - | chr7_2:84515462-84515481 | Msa1084240:CDS | 40.0% | |
| GTCCAACACTCTAACACTTA+TGG | + | chr7_2:84515467-84515486 | None:intergenic | 40.0% | |
| !!! | GTTAGAGTGTTGGACACTTT+TGG | - | chr7_2:84515472-84515491 | Msa1084240:CDS | 40.0% |
| CAGCACAAGAGTTAGAGTGT+TGG | - | chr7_2:84515393-84515412 | Msa1084240:CDS | 45.0% | |
| GGACACTTATAGGAGTTCCT+TGG | - | chr7_2:84515414-84515433 | Msa1084240:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 84515283 | 84515513 | 84515283 | ID=Msa1084240;Name=Msa1084240 |
| chr7_2 | mRNA | 84515283 | 84515513 | 84515283 | ID=Msa1084240-mRNA-1;Parent=Msa1084240;Name=Msa1084240-mRNA-1;_AED=0.73;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|76 |
| chr7_2 | exon | 84515283 | 84515513 | 84515283 | ID=Msa1084240-mRNA-1:exon:22648;Parent=Msa1084240-mRNA-1 |
| chr7_2 | CDS | 84515283 | 84515513 | 84515283 | ID=Msa1084240-mRNA-1:cds;Parent=Msa1084240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
