AlfalfaGEDB | Msa0731980

M. sativa cultivar zhongmu-4 / Msa0731980

KEGG: -
GO: -
PFAM: PF04674.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0731980	XP_013454048.2	96.190	315	12	0	1	315	25	339	0.0	622

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0731980	sp\|O82161\|PHI1_TOBAC	74.679	312	69	5	9	315	7	313	2.96e-163	459

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0731980	G7KBB9	96.190	315	12	0	1	315	1	315	0.0	621

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0731980	Msa0731990	0.874152	8.441814e-68	-8.615850e-47
Msa0731980	Msa0732010	0.894288	2.838540e-75	-8.615850e-47
Msa0731980	Msa0853250	0.827858	1.240301e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0731980	MtrunA17_Chr5g0426441	95.873	315	13	0	1	315	25	339	0.0	619
Msa0731980	MtrunA17_Chr5g0426441	95.161	310	15	0	6	315	339	648	0.0	607
Msa0731980	MtrunA17_Chr5g0426441	94.855	311	15	1	6	315	648	958	0.0	603
Msa0731980	MtrunA17_Chr3g0116001	78.912	294	49	3	22	315	24	304	3.90e-171	476
Msa0731980	MtrunA17_Chr3g0116011	78.571	294	50	4	22	315	24	304	1.42e-169	473
Msa0731980	MtrunA17_Chr3g0115951	70.533	319	88	5	1	315	1	317	6.51e-154	433
Msa0731980	MtrunA17_Chr4g0050321	61.404	285	96	3	31	315	30	300	3.71e-122	352
Msa0731980	MtrunA17_Chr2g0279311	53.355	313	130	6	4	315	7	304	1.81e-119	345
Msa0731980	MtrunA17_Chr4g0018171	35.473	296	169	8	16	300	45	329	8.97e-48	163
Msa0731980	MtrunA17_Chr1g0188071	35.714	308	173	8	18	312	46	341	4.51e-47	162

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0731980	AT4G08950.1	72.910	299	71	6	23	315	20	314	8.57e-150	423
Msa0731980	AT1G35140.1	64.331	314	106	3	1	314	1	308	3.62e-144	409
Msa0731980	AT5G64260.1	58.117	308	118	5	10	315	7	305	3.03e-121	350
Msa0731980	AT5G09440.1	59.124	274	83	7	44	315	32	278	5.45e-102	300
Msa0731980	AT5G51550.1	35.644	303	173	8	21	312	40	331	1.03e-48	166
Msa0731980	AT3G02970.1	31.438	299	169	7	46	313	37	330	4.45e-45	156
Msa0731980	AT2G17230.1	32.680	306	184	9	19	312	62	357	1.20e-42	150
Msa0731980	AT2G35150.1	34.259	324	184	11	8	311	2	316	2.04e-42	149

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Find 71 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGTTCGCGTACATTT+GGG	0.409022	5_1:+43818141	Msa0731980:CDS
AAATTTCCGTAAACCTCATT+TGG	0.356091	5_1:+43817741	Msa0731980:CDS
AAATTTGACCGGTTAGAAGA+GGG	0.580322	5_1:-43817724	None:intergenic
AACCATCAGTTGCCACGTGG+TGG	0.610757	5_1:+43817852	Msa0731980:CDS
AACCTCAGCTTGTTCTACAT+TGG	0.544398	5_1:+43818522	Msa0731980:CDS
AAGTTCGCGTACATTTGGGT+TGG	0.562836	5_1:+43818145	Msa0731980:CDS
AATAACGATGTGGGTCTTGA+TGG	0.390121	5_1:+43818256	Msa0731980:CDS
AATGTGCTGGTCAATGTGCA+TGG	0.681885	5_1:+43818182	Msa0731980:CDS
AATTCTGAAACTCAATGTGC+TGG	0.549654	5_1:+43818169	Msa0731980:CDS
ACGCAAATGGTGATAATGGA+AGG	0.560708	5_1:+43818467	Msa0731980:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTTCCGTAAACCTCATT+TGG	+	chr5_1:43817741-43817760	Msa0731980:CDS	30.0%
ATATCCATTACCAAAAGGAT+TGG	-	chr5_1:43818323-43818342	None:intergenic	30.0%
TATAACGCAAATGGTGATAA+TGG	+	chr5_1:43818463-43818482	Msa0731980:CDS	30.0%
AAATTTCCGTAAACCTCATT+TGG	+	chr5_1:43817741-43817760	Msa0731980:CDS	30.0%
ATATCCATTACCAAAAGGAT+TGG	-	chr5_1:43818323-43818342	None:intergenic	30.0%
TATAACGCAAATGGTGATAA+TGG	+	chr5_1:43818463-43818482	Msa0731980:CDS	30.0%
AGCTAGAAAACTCACTGAAT+CGG	+	chr5_1:43817670-43817689	Msa0731980:CDS	35.0%
AAATTTGACCGGTTAGAAGA+GGG	-	chr5_1:43817727-43817746	None:intergenic	35.0%
TCCATACCAAATGAGGTTTA+CGG	-	chr5_1:43817750-43817769	None:intergenic	35.0%
CTTGACGAGAATTACTCTTT+AGG	+	chr5_1:43817953-43817972	Msa0731980:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	43817602	43818549	43817602	ID=Msa0731980;Name=Msa0731980
chr5_1	mRNA	43817602	43818549	43817602	ID=Msa0731980-mRNA-1;Parent=Msa0731980;Name=Msa0731980-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|315
chr5_1	exon	43817602	43818549	43817602	ID=Msa0731980-mRNA-1:exon:12468;Parent=Msa0731980-mRNA-1
chr5_1	CDS	43817602	43818549	43817602	ID=Msa0731980-mRNA-1:cds;Parent=Msa0731980-mRNA-1

Gene Sequence

>Msa0731980

ATGGCATCTTTTAACATGAGTGCAATCTTCATTCTTTTTCTCACAATTTCACTACTTCAATTGAGTTCAGCTAGAAAACTCACTGAATCGGACCAACAACTTAAATTTCAATACCACAAAGGCCCTCTTCTAACCGGTCAAATTTCCGTAAACCTCATTTGGTATGGAAAATTCAAAACATCTCAACGAGCGATCATAACTGATTTCATTACCTCACTTTCTTCTCCTAAAAAACAAACAACCACATCACAACCATCAGTTGCCACGTGGTGGAAATCAACCGAAAAATATTACCAACTCGCCAACAACAAGAAATCAGTTAACCTAGCTCTCTCGCTAGGTACTCAAATTCTTGACGAGAATTACTCTTTAGGAAAATCTCTCACAACTAACCAAATTCTTAAACTTGCATCAAAAGGACAACAACAAAACGCCATCAACGTTGTCCTAACAGCAGCTGATGTGTTAGTTGACGGATTTTGTTCGAGTAGGTGTGGAACCCATGGTTCTTCTTATGGTGCACGCGTAAATGGAAAGAGAAACAAGTTCGCGTACATTTGGGTTGGAAATTCTGAAACTCAATGTGCTGGTCAATGTGCATGGCCATTTCACCAACCAATTTATGGTCCACAAAGTGCACCATTGGTTGCACCAAATAACGATGTGGGTCTTGATGGAATGGTTATAAATGTCGCTAGTCTTTTAGCTGGAACCGTAACCAATCCTTTTGGTAATGGATATTTTCAAGGACCAAAAGAAGCTCCTTTGGAAGCTGCTTCGGCTTGTACCGGTGTTTATGCTAAAGGAGCTTATCCTGGTTATGCTGGGGACCTTCTAGTGGACAAAACAAGTGGTGCTAGCTATAACGCAAATGGTGATAATGGAAGGAAGTATTTGTTGCCTGCGATTGCTGATCCTAAAACCTCAGCTTGTTCTACATTGGTATAA

Protein sequence

>Msa0731980

MASFNMSAIFILFLTISLLQLSSARKLTESDQQLKFQYHKGPLLTGQISVNLIWYGKFKTSQRAIITDFITSLSSPKKQTTTSQPSVATWWKSTEKYYQLANNKKSVNLALSLGTQILDENYSLGKSLTTNQILKLASKGQQQNAINVVLTAADVLVDGFCSSRCGTHGSSYGARVNGKRNKFAYIWVGNSETQCAGQCAWPFHQPIYGPQSAPLVAPNNDVGLDGMVINVASLLAGTVTNPFGNGYFQGPKEAPLEAASACTGVYAKGAYPGYAGDLLVDKTSGASYNANGDNGRKYLLPAIADPKTSACSTLV*