AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0733180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733180 MtrunA17_Chr5g0428331 89.674 184 14 3 1 183 1 180 1.75e-111 323
Msa0733180 MtrunA17_Chr5g0431811 54.783 115 50 2 164 276 629 743 2.94e-32 128
Msa0733180 MtrunA17_Chr3g0126391 53.763 93 41 2 174 264 100 192 5.02e-24 101
Msa0733180 MtrunA17_Chr8g0387981 59.459 74 30 0 201 274 128 201 8.86e-24 100
Msa0733180 MtrunA17_Chr7g0275531 40.299 134 71 2 198 330 111 236 5.29e-23 98.2
Msa0733180 MtrunA17_Chr8g0347251 59.091 66 27 0 205 270 136 201 3.10e-20 90.5
Msa0733180 MtrunA17_Chr7g0244981 41.667 108 57 2 162 264 75 181 3.45e-18 83.6
Msa0733180 MtrunA17_Chr7g0244971 37.963 108 62 1 162 264 75 182 1.31e-17 82.4
Msa0733180 MtrunA17_Chr8g0334541 52.239 67 32 0 200 266 98 164 3.10e-17 80.9
Msa0733180 MtrunA17_Chr3g0128671 36.975 119 60 3 161 271 82 193 4.19e-17 80.9
Msa0733180 MtrunA17_Chr2g0284321 42.169 83 46 1 180 262 10 90 9.46e-17 78.2
Msa0733180 MtrunA17_Chr8g0350621 47.692 65 34 0 200 264 106 170 2.20e-16 78.2
Msa0733180 MtrunA17_Chr5g0418781 42.857 105 53 4 170 273 90 188 3.26e-16 77.8
Msa0733180 MtrunA17_Chr2g0315361 53.968 63 29 0 205 267 148 210 1.01e-15 77.0
Msa0733180 MtrunA17_Chr7g0224801 56.897 58 22 1 205 262 93 147 1.33e-15 73.9
Msa0733180 MtrunA17_Chr1g0147881 43.182 88 44 3 178 262 233 317 1.37e-15 77.0
Msa0733180 MtrunA17_Chr8g0383351 52.542 59 25 1 205 263 160 215 1.40e-15 75.5
Msa0733180 MtrunA17_Chr4g0071521 47.059 85 41 3 179 262 219 300 2.30e-15 75.9
Msa0733180 MtrunA17_Chr2g0293481 39.796 98 53 2 169 262 227 322 2.46e-15 77.0
Msa0733180 MtrunA17_Chr3g0079691 53.333 60 24 2 203 262 278 333 3.55e-15 77.0
Msa0733180 MtrunA17_Chr7g0228941 54.237 59 25 1 204 262 51 107 3.96e-15 71.2
Msa0733180 MtrunA17_Chr4g0053281 45.882 85 42 3 178 262 241 321 5.13e-15 75.1
Msa0733180 MtrunA17_Chr7g0263531 49.275 69 29 3 195 262 179 242 6.96e-15 75.5
Msa0733180 MtrunA17_Chr7g0263531 49.123 57 26 1 206 262 362 415 8.01e-11 63.2
Msa0733180 MtrunA17_Chr1g0184691 45.122 82 40 3 194 273 281 359 1.24e-14 74.3
Msa0733180 MtrunA17_Chr2g0315381 43.902 82 45 1 187 268 127 207 1.28e-14 73.9
Msa0733180 MtrunA17_Chr3g0139021 54.237 59 25 1 204 262 313 369 1.29e-14 74.7
Msa0733180 MtrunA17_Chr8g0379461 47.692 65 32 1 198 262 69 131 1.86e-14 72.4
Msa0733180 MtrunA17_Chr3g0129361 53.448 58 24 1 205 262 144 198 1.95e-14 71.6
Msa0733180 MtrunA17_Chr5g0443131 54.098 61 25 1 205 265 157 214 1.98e-14 73.2
Msa0733180 MtrunA17_Chr7g0218081 48.387 62 28 2 201 262 224 281 2.53e-14 73.9
Msa0733180 MtrunA17_Chr7g0218081 48.276 58 27 1 205 262 410 464 8.10e-12 66.2
Msa0733180 MtrunA17_Chr7g0238921 52.542 59 25 1 205 263 124 179 2.93e-14 72.8
Msa0733180 MtrunA17_Chr4g0051881 55.172 58 23 1 205 262 178 232 3.65e-14 72.8
Msa0733180 MtrunA17_Chr5g0428351 45.588 68 37 0 206 273 184 251 4.23e-14 72.4
Msa0733180 MtrunA17_Chr5g0401821 42.353 85 45 3 179 262 173 254 4.85e-14 71.6
Msa0733180 MtrunA17_Chr2g0319961 40.566 106 51 4 174 274 47 145 5.14e-14 71.2
Msa0733180 MtrunA17_Chr8g0386241 50.847 59 26 1 205 263 103 158 5.65e-14 69.7
Msa0733180 MtrunA17_Chr5g0418191 47.059 68 32 2 195 262 141 204 7.17e-14 72.4
Msa0733180 MtrunA17_Chr5g0418191 46.552 58 28 1 205 262 320 374 5.33e-11 63.5
Msa0733180 MtrunA17_Chr8g0335811 53.448 58 24 1 205 262 154 208 8.33e-14 70.1
Msa0733180 MtrunA17_Chr2g0315371 49.206 63 32 0 205 267 141 203 1.26e-13 70.9
Msa0733180 MtrunA17_Chr7g0248721 53.125 64 24 3 205 266 141 200 2.21e-13 68.6
Msa0733180 MtrunA17_Chr7g0249311 50.000 58 25 2 205 262 179 232 5.77e-13 70.1
Msa0733180 MtrunA17_Chr7g0270191 40.404 99 49 3 205 296 145 240 6.64e-13 68.6
Msa0733180 MtrunA17_Chr2g0289181 49.153 59 28 1 204 262 13 69 8.02e-13 67.4
Msa0733180 MtrunA17_Chr5g0445311 48.276 58 27 1 205 262 364 418 8.58e-13 69.3
Msa0733180 MtrunA17_Chr3g0101131 48.276 58 27 1 205 262 415 469 1.11e-12 69.3
Msa0733180 MtrunA17_Chr3g0106491 36.697 109 53 4 195 302 188 281 1.50e-12 68.6
Msa0733180 MtrunA17_Chr3g0106491 46.552 58 28 1 205 262 365 419 5.33e-11 63.9
Msa0733180 MtrunA17_Chr1g0194481 51.724 58 25 1 205 262 158 212 1.57e-12 67.8
Msa0733180 MtrunA17_Chr5g0432161 48.276 58 26 2 205 262 244 297 2.07e-12 68.2
Msa0733180 MtrunA17_Chr5g0432161 48.276 58 27 1 205 262 421 475 6.72e-12 66.6
Msa0733180 MtrunA17_Chr4g0042831 35.106 94 50 2 199 292 139 221 2.91e-12 67.4
Msa0733180 MtrunA17_Chr4g0042831 48.276 58 27 1 205 262 278 332 1.52e-11 65.1
Msa0733180 MtrunA17_Chr2g0322391 53.448 58 25 1 206 263 350 405 3.25e-12 67.8
Msa0733180 MtrunA17_Chr7g0243891 44.928 69 34 2 194 262 205 269 5.38e-12 67.0
Msa0733180 MtrunA17_Chr3g0099641 50.877 57 26 1 206 262 252 306 5.77e-12 66.6
Msa0733180 MtrunA17_Chr2g0294231 44.737 76 33 2 205 280 203 269 5.95e-12 66.2
Msa0733180 MtrunA17_Chr4g0004911 44.828 58 29 1 205 262 184 238 6.09e-12 66.6
Msa0733180 MtrunA17_Chr4g0075201 46.575 73 27 2 206 278 268 328 6.31e-12 66.6
Msa0733180 MtrunA17_Chr1g0150281 49.123 57 27 1 206 262 141 195 6.39e-12 65.5
Msa0733180 MtrunA17_Chr2g0288871 52.632 57 25 1 206 262 306 360 7.78e-12 66.6
Msa0733180 MtrunA17_Chr7g0270831 52.632 57 25 1 206 262 217 271 9.03e-12 65.9
Msa0733180 MtrunA17_Chr8g0387501 52.632 57 25 1 206 262 130 184 1.11e-11 64.7
Msa0733180 MtrunA17_Chr3g0136961 44.615 65 34 1 198 262 131 193 1.58e-11 64.3
Msa0733180 MtrunA17_Chr8g0348311 52.632 57 25 1 206 262 156 210 1.86e-11 64.7
Msa0733180 MtrunA17_Chr4g0074001 47.368 57 27 1 206 262 360 413 2.18e-11 65.1
Msa0733180 MtrunA17_Chr5g0447701 47.368 57 27 1 206 262 253 306 4.35e-11 63.9
Msa0733180 MtrunA17_Chr7g0248691 41.379 87 42 3 176 262 132 209 4.86e-11 63.2
Msa0733180 MtrunA17_Chr1g0202461 49.123 57 27 1 206 262 262 316 5.86e-11 63.5
Msa0733180 MtrunA17_Chr6g0485431 50.000 58 25 2 206 262 155 209 6.66e-11 63.2
Msa0733180 MtrunA17_Chr7g0270051 47.458 59 29 1 204 262 52 108 7.71e-11 62.0
Msa0733180 MtrunA17_Chr4g0060271 52.632 57 25 1 206 262 256 310 7.98e-11 63.5
Msa0733180 MtrunA17_Chr4g0068541 45.763 59 29 1 205 263 208 263 8.96e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733180 AT2G40740.1 34.659 352 148 11 1 346 11 286 1.57e-51 173
Msa0733180 AT2G40740.3 34.659 352 148 11 1 346 20 295 4.09e-51 172
Msa0733180 AT4G11070.2 52.174 92 42 2 181 271 85 175 3.01e-22 95.5
Msa0733180 AT4G11070.1 52.174 92 42 2 181 271 117 207 4.51e-22 95.5
Msa0733180 AT4G23810.1 59.420 69 28 0 201 269 154 222 7.10e-22 95.1
Msa0733180 AT5G24110.1 45.918 98 52 1 178 275 87 183 2.51e-21 93.2
Msa0733180 AT2G40740.2 42.188 128 42 5 221 346 145 242 5.32e-21 91.3
Msa0733180 AT2G46400.1 36.429 140 80 4 149 288 56 186 7.29e-20 89.0
Msa0733180 AT3G56400.1 52.174 69 33 0 205 273 120 188 3.48e-19 87.0
Msa0733180 AT1G66550.2 53.968 63 29 0 202 264 100 162 2.78e-18 83.6
Msa0733180 AT1G66550.1 53.968 63 29 0 202 264 105 167 3.50e-18 83.6
Msa0733180 AT1G66560.1 43.956 91 44 1 174 264 79 162 7.20e-18 82.4
Msa0733180 AT1G64000.1 56.897 58 22 1 205 262 114 168 6.98e-17 78.6
Msa0733180 AT2G40750.1 53.333 60 28 0 205 264 152 211 2.89e-16 79.3
Msa0733180 AT5G41570.1 56.897 58 22 1 205 262 98 152 3.15e-16 76.3
Msa0733180 AT4G31550.2 44.048 84 44 2 179 262 220 300 4.03e-16 78.6
Msa0733180 AT5G01900.1 54.237 59 27 0 206 264 111 169 4.50e-16 77.8
Msa0733180 AT4G31550.1 48.235 85 40 3 178 262 221 301 5.06e-16 78.6
Msa0733180 AT2G30590.1 32.639 144 89 5 123 262 229 368 8.88e-16 78.2
Msa0733180 AT1G29280.1 44.086 93 41 4 176 262 42 129 1.13e-15 76.6
Msa0733180 AT2G24570.1 46.667 90 44 3 178 267 218 303 1.14e-15 77.4
Msa0733180 AT2G46130.1 55.172 58 23 1 205 262 30 84 1.40e-15 72.4
Msa0733180 AT2G23320.1 41.176 85 46 3 179 262 214 295 1.89e-15 76.6
Msa0733180 AT1G69310.4 49.254 67 30 2 205 271 147 209 2.83e-15 75.9
Msa0733180 AT1G69310.2 49.254 67 30 2 205 271 147 209 2.83e-15 75.9
Msa0733180 AT1G69310.1 49.254 67 30 2 205 271 147 209 2.83e-15 75.9
Msa0733180 AT4G26440.1 48.052 77 27 3 203 279 176 239 4.00e-15 77.0
Msa0733180 AT5G45260.1 31.343 134 88 1 133 262 1132 1265 6.44e-15 77.0
Msa0733180 AT4G18170.1 27.925 265 158 9 76 338 59 292 9.32e-15 74.7
Msa0733180 AT5G28650.1 45.882 85 42 3 179 262 236 317 9.67e-15 74.7
Msa0733180 AT4G39410.1 42.857 84 38 2 205 281 223 303 1.16e-14 74.3
Msa0733180 AT1G66600.1 36.134 119 55 3 146 264 65 162 1.57e-14 72.8
Msa0733180 AT2G34830.2 43.023 86 43 2 181 262 196 279 1.61e-14 74.7
Msa0733180 AT2G34830.1 43.023 86 43 2 181 262 187 270 1.62e-14 74.7
Msa0733180 AT5G22570.1 49.153 59 30 0 206 264 111 169 1.96e-14 73.6
Msa0733180 AT3G04670.1 41.237 97 53 3 179 274 236 329 3.24e-14 73.2
Msa0733180 AT2G37260.1 43.925 107 53 3 173 279 321 420 3.45e-14 73.9
Msa0733180 AT2G37260.1 54.386 57 22 2 206 262 166 218 1.85e-13 71.6
Msa0733180 AT3G58710.1 43.478 92 43 4 176 262 39 126 3.90e-14 72.4
Msa0733180 AT2G37260.3 43.925 107 53 3 173 279 241 340 4.15e-14 73.2
Msa0733180 AT2G37260.3 54.386 57 22 2 206 262 86 138 1.95e-13 71.2
Msa0733180 AT2G44745.1 53.448 58 24 1 205 262 145 199 4.18e-14 71.2
Msa0733180 AT2G37260.2 43.925 107 53 3 173 279 239 338 4.33e-14 73.2
Msa0733180 AT2G37260.2 54.386 57 22 2 206 262 84 136 2.04e-13 70.9
Msa0733180 AT1G29860.1 55.172 58 23 1 205 262 136 190 5.19e-14 72.0
Msa0733180 AT1G80590.1 50.847 59 29 0 206 264 86 144 6.75e-14 70.9
Msa0733180 AT1G30650.1 43.023 86 43 2 181 262 189 272 6.93e-14 72.8
Msa0733180 AT2G03340.1 48.529 68 29 3 196 262 241 303 8.52e-14 72.8
Msa0733180 AT2G03340.1 40.964 83 46 1 205 287 415 494 2.74e-11 65.1
Msa0733180 AT3G01080.3 48.387 62 28 2 201 262 201 258 8.54e-14 72.0
Msa0733180 AT3G58710.2 40.426 94 48 3 174 262 35 125 1.09e-13 70.9
Msa0733180 AT3G01080.2 48.387 62 28 2 201 262 201 258 2.74e-13 71.2
Msa0733180 AT5G46350.1 51.724 58 25 1 205 262 183 237 3.03e-13 70.5
Msa0733180 AT5G13080.1 53.448 58 24 1 205 262 67 121 3.58e-13 66.6
Msa0733180 AT3G01080.1 48.387 62 28 2 201 262 163 220 4.16e-13 70.5
Msa0733180 AT2G38470.1 43.182 88 40 4 181 262 154 237 4.19e-13 70.9
Msa0733180 AT1G13960.1 45.833 72 33 3 192 262 216 282 4.39e-13 70.9
Msa0733180 AT1G13960.1 46.552 58 28 1 205 262 409 463 9.29e-11 63.5
Msa0733180 AT1G13960.2 45.833 72 33 3 192 262 63 129 8.33e-13 69.3
Msa0733180 AT4G24240.1 41.758 91 49 3 173 262 249 336 9.16e-13 68.9
Msa0733180 AT5G52830.1 48.780 82 34 3 181 262 147 220 1.08e-12 68.9
Msa0733180 AT5G26170.1 46.552 58 28 1 205 262 113 167 1.13e-12 66.2
Msa0733180 AT4G31800.2 43.284 67 36 1 196 262 102 166 1.80e-12 67.0
Msa0733180 AT5G56270.1 45.455 77 29 3 203 279 271 334 1.98e-12 68.9
Msa0733180 AT5G49520.1 50.000 58 26 1 205 262 221 275 2.05e-12 68.2
Msa0733180 AT4G26640.7 48.276 58 27 1 205 262 341 395 2.08e-12 68.6
Msa0733180 AT4G23550.1 52.632 57 25 1 206 262 141 195 2.15e-12 67.8
Msa0733180 AT4G04450.2 42.045 88 38 3 206 293 199 273 2.24e-12 68.2
Msa0733180 AT4G26640.2 48.276 58 27 1 205 262 381 435 2.30e-12 68.6
Msa0733180 AT4G26640.5 48.276 58 27 1 205 262 205 259 2.32e-12 68.2
Msa0733180 AT4G26640.6 48.276 58 27 1 205 262 205 259 2.32e-12 68.2
Msa0733180 AT4G26640.4 48.276 58 27 1 205 262 205 259 2.32e-12 68.2
Msa0733180 AT4G26640.3 48.276 58 27 1 205 262 205 259 2.32e-12 68.2
Msa0733180 AT4G26640.1 48.276 58 27 1 205 262 309 363 2.48e-12 68.2
Msa0733180 AT4G31800.1 43.284 67 36 1 196 262 167 231 2.54e-12 67.4
Msa0733180 AT4G04450.1 42.045 88 38 3 206 293 293 367 3.12e-12 68.2
Msa0733180 AT4G30935.1 42.857 77 40 2 206 282 332 404 3.75e-12 67.8
Msa0733180 AT2G47260.1 38.462 91 53 1 180 270 149 236 7.46e-12 66.2
Msa0733180 AT4G01250.1 38.554 83 44 2 180 262 108 183 1.15e-11 65.5
Msa0733180 AT1G62300.1 52.632 57 25 1 206 262 313 367 1.82e-11 65.9
Msa0733180 AT4G01720.1 52.632 57 25 1 206 262 240 294 2.64e-11 65.1
Msa0733180 AT2G30250.1 52.632 57 24 1 206 262 329 382 3.42e-11 64.7
Msa0733180 AT5G43290.1 49.153 59 27 1 205 263 114 169 3.45e-11 63.5
Msa0733180 AT5G45050.1 42.353 85 42 2 178 262 1158 1235 4.06e-11 65.1
Msa0733180 AT5G45050.2 42.353 85 42 2 178 262 1130 1207 4.19e-11 65.1
Msa0733180 AT1G55600.1 44.776 67 34 1 196 262 298 361 4.34e-11 64.3
Msa0733180 AT4G22070.4 52.632 57 25 1 206 262 298 352 4.60e-11 64.7
Msa0733180 AT4G22070.3 52.632 57 25 1 206 262 298 352 4.60e-11 64.7
Msa0733180 AT4G22070.2 52.632 57 25 1 206 262 298 352 4.60e-11 64.7
Msa0733180 AT4G22070.1 52.632 57 25 1 206 262 298 352 4.60e-11 64.7
Msa0733180 AT5G45050.4 42.353 85 42 2 178 262 1019 1096 4.63e-11 65.1
Msa0733180 AT2G04880.2 47.368 57 27 1 206 262 284 337 5.04e-11 64.3
Msa0733180 AT2G04880.2 51.724 58 24 2 205 262 111 164 9.89e-11 63.2
Msa0733180 AT2G04880.1 47.368 57 27 1 206 262 308 361 5.40e-11 64.3
Msa0733180 AT2G04880.1 51.724 58 24 2 205 262 111 164 9.09e-11 63.5
Msa0733180 AT2G25000.2 37.500 80 48 1 183 262 81 158 5.98e-11 62.4
Msa0733180 AT2G25000.4 37.500 80 48 1 183 262 81 158 5.98e-11 62.4
Msa0733180 AT2G25000.3 37.500 80 48 1 183 262 103 180 8.96e-11 62.0

Find 96 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5_1:-46239201 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5_1:-46242003 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5_1:-46242035 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.259697 5_1:-46239253 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5_1:+46239582 Msa0733180:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5_1:+46241297 Msa0733180:CDS
TGAAATGGGTGGGAGGGATA+TGG 0.320333 5_1:+46239551 Msa0733180:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.320492 5_1:+46239536 Msa0733180:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5_1:+46242244 Msa0733180:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5_1:+46239363 Msa0733180:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5_1:-46242163 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5_1:-46241999 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5_1:-46239184 None:intergenic
CCTTTATCAGAAGTTACTAT+AGG 0.370143 5_1:+46241949 Msa0733180:intron
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5_1:+46242289 Msa0733180:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5_1:-46241288 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.382607 5_1:+46239511 Msa0733180:CDS
ATTAGAACACTCACATCAAA+AGG 0.383819 5_1:-46239450 None:intergenic
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5_1:-46239315 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5_1:+46239573 Msa0733180:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5_1:+46239404 Msa0733180:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5_1:+46241212 Msa0733180:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5_1:+46241286 Msa0733180:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5_1:-46239183 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5_1:+46242232 Msa0733180:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5_1:+46242177 Msa0733180:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5_1:-46241255 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5_1:-46242141 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5_1:-46242034 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5_1:+46239372 Msa0733180:CDS
CGTTCAGTTGTTAAGGTCTA+GGG 0.444913 5_1:+46239512 Msa0733180:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5_1:-46242162 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5_1:-46242116 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.450200 5_1:+46239537 Msa0733180:CDS
GAACATTACATCGGCCATAT+CGG 0.452060 5_1:-46242258 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5_1:-46242107 None:intergenic
ATATGATGATGGTGCTGTTA+AGG 0.457432 5_1:-46242084 None:intergenic
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5_1:-46242153 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5_1:-46239115 None:intergenic
GGTGGTGGTGGATATGATGA+TGG 0.483740 5_1:-46242095 None:intergenic
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5_1:-46242176 None:intergenic
GGACCCTCTACTTCAAGATT+CGG 0.492644 5_1:+46242218 Msa0733180:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5_1:-46242187 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5_1:+46242194 Msa0733180:CDS
TCTAGGGGAACTTTGAGGAT+TGG 0.506422 5_1:+46239528 Msa0733180:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5_1:+46241249 Msa0733180:CDS
TAAGGTCTAGGGGAACTTTG+AGG 0.511706 5_1:+46239523 Msa0733180:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5_1:+46239505 Msa0733180:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5_1:+46242188 Msa0733180:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5_1:+46242160 Msa0733180:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5_1:-46239200 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5_1:+46242041 Msa0733180:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5_1:-46241261 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5_1:+46239366 Msa0733180:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5_1:+46241276 Msa0733180:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5_1:-46239193 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5_1:+46239483 Msa0733180:CDS
AAGGTTTAGATAGATCAAAA+GGG 0.540645 5_1:+46239574 Msa0733180:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5_1:+46239471 Msa0733180:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5_1:+46242146 Msa0733180:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5_1:-46242121 None:intergenic
TGCGGTGAACCACGACCTCA+GGG 0.552440 5_1:+46242178 Msa0733180:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5_1:-46242173 None:intergenic
TACTTGGCCGCAAGCACCCA+AGG 0.555851 5_1:+46241331 Msa0733180:CDS
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5_1:-46242113 None:intergenic
TAATTTACCTTGGGTGCTTG+CGG 0.559902 5_1:-46241338 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5_1:+46239540 Msa0733180:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5_1:+46242197 Msa0733180:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5_1:+46239541 Msa0733180:CDS
GGCCGATGTAATGTTCAACT+CGG 0.582893 5_1:+46242265 Msa0733180:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5_1:+46242147 Msa0733180:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5_1:+46239544 Msa0733180:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5_1:-46242221 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5_1:-46239449 None:intergenic
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5_1:+46242150 Msa0733180:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5_1:+46242278 Msa0733180:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.607995 5_1:+46239545 Msa0733180:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5_1:-46239325 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5_1:+46242334 Msa0733180:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5_1:-46242110 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5_1:+46242266 Msa0733180:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5_1:-46242222 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5_1:-46241285 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5_1:+46239474 Msa0733180:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5_1:+46239477 Msa0733180:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5_1:-46242251 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5_1:+46242182 Msa0733180:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5_1:+46239486 Msa0733180:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5_1:+46239170 Msa0733180:CDS
GTTCAGTTGTTAAGGTCTAG+GGG 0.665415 5_1:+46239513 Msa0733180:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5_1:+46242235 Msa0733180:CDS
ATGGGTGGGAGGGATATGGA+AGG 0.678067 5_1:+46239555 Msa0733180:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5_1:-46242193 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5_1:-46242267 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5_1:+46242179 Msa0733180:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5_1:-46239197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAATCAATTTTTTTTTTA+TGG - chr5_1:46240736-46240755 None:intergenic 10.0%
!!! AAAATTGATTTGAATTTTTT+TGG + chr5_1:46240743-46240762 Msa0733180:intron 10.0%
!!! TTTTACATAAAATTATAAGT+AGG + chr5_1:46241113-46241132 Msa0733180:intron 10.0%
!!! TGTTTATTTTTTAAATATCT+TGG + chr5_1:46241884-46241903 Msa0733180:intron 10.0%
!!! GTTTTTATTATATAATTTGT+TGG + chr5_1:46241906-46241925 Msa0733180:intron 10.0%
!!! TTATATACATACCATTTTTT+TGG - chr5_1:46239645-46239664 None:intergenic 15.0%
!! TCAAAGTTTCATATAATAAA+TGG + chr5_1:46239727-46239746 Msa0733180:intron 15.0%
!!! TCACTAGTTAATTATTTTAA+TGG + chr5_1:46240922-46240941 Msa0733180:intron 15.0%
!!! AGTAAAGTGAATTTTTTTTT+TGG - chr5_1:46241026-46241045 None:intergenic 15.0%
!! AATTATTGTTAATTTACCTT+GGG - chr5_1:46241350-46241369 None:intergenic 15.0%
!! TAATTATTGTTAATTTACCT+TGG - chr5_1:46241351-46241370 None:intergenic 15.0%
!! AGAATTGTACTTTATAATAT+AGG + chr5_1:46241374-46241393 Msa0733180:intron 15.0%
!! GAATTGTACTTTATAATATA+GGG + chr5_1:46241375-46241394 Msa0733180:intron 15.0%
!! AAATATTTAACAAGTAATCA+AGG - chr5_1:46241697-46241716 None:intergenic 15.0%
!!! ATTTCTAAGGTATTGAATTT+AGG + chr5_1:46239836-46239855 Msa0733180:intron 20.0%
!! AAAAATCTTATGGAAATGAT+TGG - chr5_1:46240237-46240256 None:intergenic 20.0%
!! TTTCTTTCAATTATTGTTGT+TGG - chr5_1:46240457-46240476 None:intergenic 20.0%
!! AAATTTATATATGCAAGATC+CGG + chr5_1:46240973-46240992 Msa0733180:intron 20.0%
!! AATAGTTTGACACAATTATT+AGG + chr5_1:46241050-46241069 Msa0733180:intron 20.0%
!! CAATTATTAGGAAGTTAATA+TGG + chr5_1:46241062-46241081 Msa0733180:intron 20.0%
!! AAGAAATATTCTTAGAAGAA+AGG - chr5_1:46241483-46241502 None:intergenic 20.0%
!!! TTTCTTCTAAGAATATTTCT+TGG + chr5_1:46241482-46241501 Msa0733180:intron 20.0%
!! ACTATTTACATTCATGTATA+AGG + chr5_1:46241583-46241602 Msa0733180:intron 20.0%
!! CTATTTACATTCATGTATAA+GGG + chr5_1:46241584-46241603 Msa0733180:intron 20.0%
!!! TTTTATCTAACTGAAGATAA+AGG + chr5_1:46242320-46242339 Msa0733180:CDS 20.0%
! ATTGAGAATAAGATGATAAG+TGG + chr5_1:46239471-46239490 Msa0733180:CDS 25.0%
! AAGGTTTAGATAGATCAAAA+GGG + chr5_1:46239574-46239593 Msa0733180:CDS 25.0%
! ATTCAATAACATAGATTGTG+AGG - chr5_1:46239765-46239784 None:intergenic 25.0%
! ATGTTATTGAATCAAAGTCT+TGG + chr5_1:46239773-46239792 Msa0733180:intron 25.0%
!!! CAACACAAAGATTATTTCTA+AGG + chr5_1:46239823-46239842 Msa0733180:intron 25.0%
!!! CATATTTGGGTTTTAGTTTA+TGG + chr5_1:46239916-46239935 Msa0733180:intron 25.0%
! TTGCAATTCAACTGAAATTA+AGG - chr5_1:46240006-46240025 None:intergenic 25.0%
! ATACATCAAAGTTGAAAGTT+AGG - chr5_1:46240078-46240097 None:intergenic 25.0%
!! TAGGGTGAAAATTTTCATTA+TGG - chr5_1:46240174-46240193 None:intergenic 25.0%
! TAATGTGAAAATCCTAAGTA+GGG - chr5_1:46240192-46240211 None:intergenic 25.0%
! ATAATGTGAAAATCCTAAGT+AGG - chr5_1:46240193-46240212 None:intergenic 25.0%
!! TTCCATAAGATTTTTACTAG+AGG + chr5_1:46240242-46240261 Msa0733180:intron 25.0%
!! TCCATAAGATTTTTACTAGA+GGG + chr5_1:46240243-46240262 Msa0733180:intron 25.0%
! TAAAAGTAGAGATTGAAATG+AGG + chr5_1:46240299-46240318 Msa0733180:intron 25.0%
!! ATGTAAGATAGAATTTTCCA+TGG + chr5_1:46240577-46240596 Msa0733180:intron 25.0%
!!! TTTTTTGGTGTTTGCTATTT+TGG + chr5_1:46240758-46240777 Msa0733180:intron 25.0%
! AATTTATATATGCAAGATCC+GGG + chr5_1:46240974-46240993 Msa0733180:intron 25.0%
!!! TTTTTTTTTTGGTGATGACT+GGG - chr5_1:46241015-46241034 None:intergenic 25.0%
!!! ATTTTTTTTTTGGTGATGAC+TGG - chr5_1:46241016-46241035 None:intergenic 25.0%
! GTATAATTCAGTTGTTAGAT+TGG + chr5_1:46241084-46241103 Msa0733180:intron 25.0%
! TGAAACTTTCAAACTCTTTA+TGG - chr5_1:46241410-46241429 None:intergenic 25.0%
!! ATTCTTAGAAGAAAGGTATA+AGG - chr5_1:46241476-46241495 None:intergenic 25.0%
!! CTGGACACAAATATAATTTT+TGG - chr5_1:46241983-46242002 None:intergenic 25.0%
!! ATGTTACTTCAACTATGTTA+GGG - chr5_1:46242037-46242056 None:intergenic 25.0%
!! TATGTTACTTCAACTATGTT+AGG - chr5_1:46242038-46242057 None:intergenic 25.0%
! ATAGTTGAAGTAACATATAG+AGG + chr5_1:46242041-46242060 Msa0733180:CDS 25.0%
!!! TGCTTTTTCTACATCATCAA+TGG - chr5_1:46239223-46239242 None:intergenic 30.0%
CATCATCATTACTCTTTCTT+TGG - chr5_1:46239256-46239275 None:intergenic 30.0%
ATTTGAGCAAAGGAATTAGA+AGG - chr5_1:46239318-46239337 None:intergenic 30.0%
AAAACTCAACATGCTCAAAT+TGG + chr5_1:46239363-46239382 Msa0733180:CDS 30.0%
TTAGAACACTCACATCAAAA+GGG - chr5_1:46239452-46239471 None:intergenic 30.0%
ATTAGAACACTCACATCAAA+AGG - chr5_1:46239453-46239472 None:intergenic 30.0%
GAAGGTTTAGATAGATCAAA+AGG + chr5_1:46239573-46239592 Msa0733180:CDS 30.0%
!!! TACCATTTTTTTGGTCTTGA+AGG - chr5_1:46239636-46239655 None:intergenic 30.0%
ACCGGGTAATTTACATATTT+GGG + chr5_1:46239903-46239922 Msa0733180:intron 30.0%
ACCCAAATATGTAAATTACC+CGG - chr5_1:46239907-46239926 None:intergenic 30.0%
!!! GGGTTTTAGTTTATGGTTAT+AGG + chr5_1:46239923-46239942 Msa0733180:intron 30.0%
! TTCTGTAAGATTTTCACTAG+AGG + chr5_1:46240144-46240163 Msa0733180:intron 30.0%
! TCTGTAAGATTTTCACTAGA+GGG + chr5_1:46240145-46240164 Msa0733180:intron 30.0%
! TGAAAATTTTCACCCTACTT+AGG + chr5_1:46240177-46240196 Msa0733180:intron 30.0%
TCCCTCTAGTAAAAATCTTA+TGG - chr5_1:46240247-46240266 None:intergenic 30.0%
GATTACTCAATGTTTGTACT+AGG - chr5_1:46240279-46240298 None:intergenic 30.0%
AGATTGAAATGAGGTTAACT+TGG + chr5_1:46240308-46240327 Msa0733180:intron 30.0%
! AGGTCACTAGTTTATGAAAA+TGG + chr5_1:46240374-46240393 Msa0733180:intron 30.0%
TCTACAATTGTGACAAAAAC+AGG - chr5_1:46240416-46240435 None:intergenic 30.0%
! CTTTCAATTATTGTTGTTGG+CGG - chr5_1:46240454-46240473 None:intergenic 30.0%
! ACAAAAGTGAAATGCTTTTG+AGG + chr5_1:46240481-46240500 Msa0733180:intron 30.0%
! GGTATTGAAAGCTTTAGTTT+AGG + chr5_1:46240502-46240521 Msa0733180:intron 30.0%
AACACACCAATAATAACGTA+CGG - chr5_1:46240530-46240549 None:intergenic 30.0%
CTAATTACTAAGAAAGACCA+TGG - chr5_1:46240597-46240616 None:intergenic 30.0%
CATGGTCTTTCTTAGTAATT+AGG + chr5_1:46240595-46240614 Msa0733180:intron 30.0%
TAGTTTACATGACCAAAACA+AGG + chr5_1:46240668-46240687 Msa0733180:intron 30.0%
! AGGTCTTTGCTATAGTATTT+AGG + chr5_1:46240688-46240707 Msa0733180:intron 30.0%
ATTTATATATGCAAGATCCG+GGG + chr5_1:46240975-46240994 Msa0733180:intron 30.0%
!!! TTTTTTTTTGGTGATGACTG+GGG - chr5_1:46241014-46241033 None:intergenic 30.0%
TATGGCCAAAAAGAAATACT+TGG + chr5_1:46241315-46241334 Msa0733180:CDS 30.0%
!! AAGAAAGGTATAAGGTTTTC+TGG - chr5_1:46241468-46241487 None:intergenic 30.0%
TGTGAATGAGTCAATACATA+GGG - chr5_1:46241543-46241562 None:intergenic 30.0%
CCTATAGTAACTTCTGATAA+AGG - chr5_1:46241952-46241971 None:intergenic 30.0%
CCTTTATCAGAAGTTACTAT+AGG + chr5_1:46241949-46241968 Msa0733180:intron 30.0%
TGGATGAGAATAGCATTTCA+GGG - chr5_1:46239203-46239222 None:intergenic 35.0%
ATGGATGAGAATAGCATTTC+AGG - chr5_1:46239204-46239223 None:intergenic 35.0%
ATGAGACAGAATTTGAGCAA+AGG - chr5_1:46239328-46239347 None:intergenic 35.0%
TTCAATGCATGCACATTCAA+TGG + chr5_1:46239404-46239423 Msa0733180:CDS 35.0%
GAGAATAAGATGATAAGTGG+CGG + chr5_1:46239474-46239493 Msa0733180:CDS 35.0%
! AACTTTGAGGATTGGTGAAA+TGG + chr5_1:46239536-46239555 Msa0733180:CDS 35.0%
! ACTTTGAGGATTGGTGAAAT+GGG + chr5_1:46239537-46239556 Msa0733180:CDS 35.0%
GATAGATCAAAAGGGTTAGA+AGG + chr5_1:46239582-46239601 Msa0733180:CDS 35.0%
!!! CATTTTTTTGGTCTTGAAGG+TGG - chr5_1:46239633-46239652 None:intergenic 35.0%
CACCTTCAAGACCAAAAAAA+TGG + chr5_1:46239631-46239650 Msa0733180:CDS 35.0%
! GGTATTGAATTTAGGTGTTG+AGG + chr5_1:46239844-46239863 Msa0733180:intron 35.0%
! TTTCAGTGTTTTTCCAACAC+CGG + chr5_1:46239885-46239904 Msa0733180:intron 35.0%
ATATGTAAATTACCCGGTGT+TGG - chr5_1:46239901-46239920 None:intergenic 35.0%
CACCGGGTAATTTACATATT+TGG + chr5_1:46239902-46239921 Msa0733180:intron 35.0%
TTATAGGTGCCTTGCTAATT+GGG + chr5_1:46239939-46239958 Msa0733180:intron 35.0%
TTGCGTTAAGATGTGACATA+TGG + chr5_1:46239978-46239997 Msa0733180:intron 35.0%
TGCGTTAAGATGTGACATAT+GGG + chr5_1:46239979-46239998 Msa0733180:intron 35.0%
ATGTGTGTGTGATTGAATCT+AGG + chr5_1:46240338-46240357 Msa0733180:intron 35.0%
AACTAGTGACCTAATTAGCA+AGG - chr5_1:46240366-46240385 None:intergenic 35.0%
TAGGATCCGTACGTTATTAT+TGG + chr5_1:46240521-46240540 Msa0733180:intron 35.0%
TGGTGTGTTTGTAAACAGAA+AGG + chr5_1:46240541-46240560 Msa0733180:intron 35.0%
AATTAGGCAACAAGTCATCA+TGG + chr5_1:46240611-46240630 Msa0733180:intron 35.0%
!! CTATAGCAAAGACCTTGTTT+TGG - chr5_1:46240683-46240702 None:intergenic 35.0%
GTGTCTAAGAACTGTGTATT+TGG + chr5_1:46240784-46240803 Msa0733180:intron 35.0%
TCACACACAAACACACATAT+AGG - chr5_1:46241154-46241173 None:intergenic 35.0%
! ATGTGAAGGCACAACAAATT+AGG - chr5_1:46241185-46241204 None:intergenic 35.0%
GGCTTTACTTGGAGAAAATA+TGG + chr5_1:46241297-46241316 Msa0733180:CDS 35.0%
! AAGGTATAAGGTTTTCTGGA+TGG - chr5_1:46241464-46241483 None:intergenic 35.0%
GTGTGAATGAGTCAATACAT+AGG - chr5_1:46241544-46241563 None:intergenic 35.0%
!! ATATGATGATGGTGCTGTTA+AGG - chr5_1:46242087-46242106 None:intergenic 35.0%
CTAATGTTCACAAGTAGGTT+TGG - chr5_1:46242119-46242138 None:intergenic 35.0%
TGGTACTAATGTTCACAAGT+AGG - chr5_1:46242124-46242143 None:intergenic 35.0%
!! AAGATTCGGCATTGATTATC+CGG + chr5_1:46242232-46242251 Msa0733180:CDS 35.0%
TGAACTAGAACTACCCAACA+TGG + chr5_1:46239170-46239189 Msa0733180:CDS 40.0%
ATGAGAATAGCATTTCAGGG+TGG - chr5_1:46239200-46239219 None:intergenic 40.0%
ACTCAACATGCTCAAATTGG+TGG + chr5_1:46239366-46239385 Msa0733180:CDS 40.0%
AATAAGATGATAAGTGGCGG+TGG + chr5_1:46239477-46239496 Msa0733180:CDS 40.0%
ACGTTCAGTTGTTAAGGTCT+AGG + chr5_1:46239511-46239530 Msa0733180:CDS 40.0%
CGTTCAGTTGTTAAGGTCTA+GGG + chr5_1:46239512-46239531 Msa0733180:CDS 40.0%
GTTCAGTTGTTAAGGTCTAG+GGG + chr5_1:46239513-46239532 Msa0733180:CDS 40.0%
! TTCAGTGTTTTTCCAACACC+GGG + chr5_1:46239886-46239905 Msa0733180:intron 40.0%
GTTATAGGTGCCTTGCTAAT+TGG + chr5_1:46239938-46239957 Msa0733180:intron 40.0%
ATCTAGTTGCCCAATTAGCA+AGG - chr5_1:46239951-46239970 None:intergenic 40.0%
CAAAGTTGAAAGTTAGGTCG+TGG - chr5_1:46240072-46240091 None:intergenic 40.0%
TCAATCACACACACATTGTG+AGG - chr5_1:46240334-46240353 None:intergenic 40.0%
ATCTAGGTGCCTTGCTAATT+AGG + chr5_1:46240354-46240373 Msa0733180:intron 40.0%
TAGCAGCAGGAAGAATGATT+TGG + chr5_1:46241212-46241231 Msa0733180:intron 40.0%
ATCTCAGTGTTTCCAATCTG+AGG - chr5_1:46241264-46241283 None:intergenic 40.0%
! TGCGGCCAAGTATTTCTTTT+TGG - chr5_1:46241323-46241342 None:intergenic 40.0%
TAATTTACCTTGGGTGCTTG+CGG - chr5_1:46241341-46241360 None:intergenic 40.0%
AAGTCTTTGCACTTGCTTCT+TGG - chr5_1:46242006-46242025 None:intergenic 40.0%
ATGTTCACAAGTAGGTTTGG+TGG - chr5_1:46242116-46242135 None:intergenic 40.0%
GGAAGAAATGGCAGTTTCAA+TGG - chr5_1:46242144-46242163 None:intergenic 40.0%
! AAACTGCCATTTCTTCCCAA+GGG + chr5_1:46242147-46242166 Msa0733180:CDS 40.0%
ATGCCGAATCTTGAAGTAGA+GGG - chr5_1:46242224-46242243 None:intergenic 40.0%
AATGCCGAATCTTGAAGTAG+AGG - chr5_1:46242225-46242244 None:intergenic 40.0%
GAACATTACATCGGCCATAT+CGG - chr5_1:46242261-46242280 None:intergenic 40.0%
! ACCCGAGTTGAACATTACAT+CGG - chr5_1:46242270-46242289 None:intergenic 40.0%
TAGTAGCAGTGGCAATAGTA+TGG + chr5_1:46242289-46242308 Msa0733180:CDS 40.0%
AGATAAAGGTGAACCTGATG+AGG + chr5_1:46242334-46242353 Msa0733180:CDS 40.0%
GAATAGCATTTCAGGGTGGT+TGG - chr5_1:46239196-46239215 None:intergenic 45.0%
CATGCTCAAATTGGTGGAAG+TGG + chr5_1:46239372-46239391 Msa0733180:CDS 45.0%
TAAGGTCTAGGGGAACTTTG+AGG + chr5_1:46239523-46239542 Msa0733180:CDS 45.0%
! TCTAGGGGAACTTTGAGGAT+TGG + chr5_1:46239528-46239547 Msa0733180:CDS 45.0%
! TTGAGGATTGGTGAAATGGG+TGG + chr5_1:46239540-46239559 Msa0733180:CDS 45.0%
! TGAGGATTGGTGAAATGGGT+GGG + chr5_1:46239541-46239560 Msa0733180:CDS 45.0%
GCTGTCTTGTGTGATTGATG+AGG - chr5_1:46240643-46240662 None:intergenic 45.0%
TGTTGTGCCTTCACATGCAT+TGG + chr5_1:46241189-46241208 Msa0733180:intron 45.0%
CAAGTAAAGCCATCTTCTGG+TGG - chr5_1:46241288-46241307 None:intergenic 45.0%
! CACCAGAAGATGGCTTTACT+TGG + chr5_1:46241286-46241305 Msa0733180:CDS 45.0%
CTCCAAGTAAAGCCATCTTC+TGG - chr5_1:46241291-46241310 None:intergenic 45.0%
!! TTCACAAGTAGGTTTGGTGG+TGG - chr5_1:46242113-46242132 None:intergenic 45.0%
! GAAACTGCCATTTCTTCCCA+AGG + chr5_1:46242146-46242165 Msa0733180:CDS 45.0%
GGACCCTCTACTTCAAGATT+CGG + chr5_1:46242218-46242237 Msa0733180:CDS 45.0%
!! ATTCGGCATTGATTATCCGG+TGG + chr5_1:46242235-46242254 Msa0733180:CDS 45.0%
GGCCGATGTAATGTTCAACT+CGG + chr5_1:46242265-46242284 Msa0733180:CDS 45.0%
GCCGATGTAATGTTCAACTC+GGG + chr5_1:46242266-46242285 Msa0733180:CDS 45.0%
ATGATAAGTGGCGGTGGTGA+TGG + chr5_1:46239483-46239502 Msa0733180:CDS 50.0%
GCGGTGACGTTCAGTTGTTA+AGG + chr5_1:46239505-46239524 Msa0733180:CDS 50.0%
GATTGGTGAAATGGGTGGGA+GGG + chr5_1:46239545-46239564 Msa0733180:CDS 50.0%
TGAAATGGGTGGGAGGGATA+TGG + chr5_1:46239551-46239570 Msa0733180:CDS 50.0%
GCTGCTACCAATGCATGTGA+AGG - chr5_1:46241199-46241218 None:intergenic 50.0%
! TCACATGCATTGGTAGCAGC+AGG + chr5_1:46241199-46241218 Msa0733180:intron 50.0%
ATGCATCCAGCACCTCAGAT+TGG + chr5_1:46241249-46241268 Msa0733180:CDS 50.0%
! GTGTTTCCAATCTGAGGTGC+TGG - chr5_1:46241258-46241277 None:intergenic 50.0%
ACTGAGATGCCACCAGAAGA+TGG + chr5_1:46241276-46241295 Msa0733180:CDS 50.0%
CTTTGCACTTGCTTCTTGGC+TGG - chr5_1:46242002-46242021 None:intergenic 50.0%
!! GGTGGTGGTGGATATGATGA+TGG - chr5_1:46242098-46242117 None:intergenic 50.0%
!! ACAAGTAGGTTTGGTGGTGG+TGG - chr5_1:46242110-46242129 None:intergenic 50.0%
TGATTATCCGGTGGCCGATA+TGG + chr5_1:46242244-46242263 Msa0733180:CDS 50.0%
TTCAACTCGGGTAGTAGCAG+TGG + chr5_1:46242278-46242297 Msa0733180:CDS 50.0%
! TCAGGGTGGTTGGCCATGTT+GGG - chr5_1:46239186-46239205 None:intergenic 55.0%
! TTCAGGGTGGTTGGCCATGT+TGG - chr5_1:46239187-46239206 None:intergenic 55.0%
!! ATAAGTGGCGGTGGTGATGG+CGG + chr5_1:46239486-46239505 Msa0733180:CDS 55.0%
! GGATTGGTGAAATGGGTGGG+AGG + chr5_1:46239544-46239563 Msa0733180:CDS 55.0%
ATGGGTGGGAGGGATATGGA+AGG + chr5_1:46239555-46239574 Msa0733180:CDS 55.0%
!! TTGGTGATGACTGGGGTTCG+AGG - chr5_1:46241007-46241026 None:intergenic 55.0%
! CTGCCATTTCTTCCCAAGGG+TGG + chr5_1:46242150-46242169 Msa0733180:CDS 55.0%
TGGCCACCCTTGGGAAGAAA+TGG - chr5_1:46242156-46242175 None:intergenic 55.0%
GGGGTTCGAGGTTTGAACCC+CGG - chr5_1:46240995-46241014 None:intergenic 60.0%
TACTTGGCCGCAAGCACCCA+AGG + chr5_1:46241331-46241350 Msa0733180:CDS 60.0%
TGCGGTGAACCACGACCTCA+GGG + chr5_1:46242178-46242197 Msa0733180:CDS 60.0%
ACATCGGCCATATCGGCCAC+CGG - chr5_1:46242254-46242273 None:intergenic 60.0%
TTCCCAAGGGTGGCCACCTG+CGG + chr5_1:46242160-46242179 Msa0733180:CDS 65.0%
AGGTCGTGGTTCACCGCAGG+TGG - chr5_1:46242176-46242195 None:intergenic 65.0%
CTGAGGTCGTGGTTCACCGC+AGG - chr5_1:46242179-46242198 None:intergenic 65.0%
CTGCGGTGAACCACGACCTC+AGG + chr5_1:46242177-46242196 Msa0733180:CDS 65.0%
GCGGTGAACCACGACCTCAG+GGG + chr5_1:46242179-46242198 Msa0733180:CDS 65.0%
GTGAACCACGACCTCAGGGG+CGG + chr5_1:46242182-46242201 Msa0733180:CDS 65.0%
CACCGCAGGTGGCCACCCTT+GGG - chr5_1:46242165-46242184 None:intergenic 70.0%
TCACCGCAGGTGGCCACCCT+TGG - chr5_1:46242166-46242185 None:intergenic 70.0%
CACGACCTCAGGGGCGGAGA+CGG + chr5_1:46242188-46242207 Msa0733180:CDS 70.0%
CAGTACCGTCTCCGCCCCTG+AGG - chr5_1:46242196-46242215 None:intergenic 70.0%
CTCAGGGGCGGAGACGGTAC+TGG + chr5_1:46242194-46242213 Msa0733180:CDS 70.0%
! AGGGGCGGAGACGGTACTGG+TGG + chr5_1:46242197-46242216 Msa0733180:CDS 70.0%
CGTCTCCGCCCCTGAGGTCG+TGG - chr5_1:46242190-46242209 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 46239117 46242367 46239117 ID=Msa0733180;Name=Msa0733180
chr5_1 mRNA 46239117 46242367 46239117 ID=Msa0733180-mRNA-1;Parent=Msa0733180;Name=Msa0733180-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|360
chr5_1 exon 46239117 46239652 46239117 ID=Msa0733180-mRNA-1:exon:13179;Parent=Msa0733180-mRNA-1
chr5_1 exon 46241215 46241352 46241215 ID=Msa0733180-mRNA-1:exon:13180;Parent=Msa0733180-mRNA-1
chr5_1 exon 46241959 46242367 46241959 ID=Msa0733180-mRNA-1:exon:13181;Parent=Msa0733180-mRNA-1
chr5_1 CDS 46239117 46239652 46239117 ID=Msa0733180-mRNA-1:cds;Parent=Msa0733180-mRNA-1
chr5_1 CDS 46241215 46241352 46241215 ID=Msa0733180-mRNA-1:cds;Parent=Msa0733180-mRNA-1
chr5_1 CDS 46241959 46242367 46241959 ID=Msa0733180-mRNA-1:cds;Parent=Msa0733180-mRNA-1
Gene Sequence

>Msa0733180

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGTTGTCATCACAACATGACACAACAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGGAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATATGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGCACCCAAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCCTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>Msa0733180

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMLSSQHDTTTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRGTLRIGEMGGRDMEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKHPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN*