AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0774220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0774220 MtrunA17_Chr5g0428331 89.130 184 15 3 1 183 1 180 3.26e-110 319
Msa0774220 MtrunA17_Chr5g0431811 54.783 115 50 2 164 276 629 743 3.00e-32 128
Msa0774220 MtrunA17_Chr3g0126391 53.763 93 41 2 174 264 100 192 4.37e-24 101
Msa0774220 MtrunA17_Chr8g0387981 59.459 74 30 0 201 274 128 201 8.18e-24 100
Msa0774220 MtrunA17_Chr7g0275531 40.299 134 71 2 198 330 111 236 5.45e-23 98.2
Msa0774220 MtrunA17_Chr8g0347251 59.091 66 27 0 205 270 136 201 3.01e-20 90.5
Msa0774220 MtrunA17_Chr7g0244981 41.667 108 57 2 162 264 75 181 3.58e-18 83.6
Msa0774220 MtrunA17_Chr7g0244971 37.963 108 62 1 162 264 75 182 1.38e-17 82.0
Msa0774220 MtrunA17_Chr8g0334541 52.239 67 32 0 200 266 98 164 3.13e-17 80.9
Msa0774220 MtrunA17_Chr3g0128671 36.975 119 60 3 161 271 82 193 3.96e-17 81.3
Msa0774220 MtrunA17_Chr2g0284321 42.169 83 46 1 180 262 10 90 9.09e-17 78.2
Msa0774220 MtrunA17_Chr8g0350621 47.692 65 34 0 200 264 106 170 2.22e-16 78.2
Msa0774220 MtrunA17_Chr5g0418781 42.857 105 53 4 170 273 90 188 3.11e-16 78.2
Msa0774220 MtrunA17_Chr2g0315361 53.968 63 29 0 205 267 148 210 9.48e-16 77.0
Msa0774220 MtrunA17_Chr7g0224801 56.897 58 22 1 205 262 93 147 1.27e-15 73.9
Msa0774220 MtrunA17_Chr1g0147881 43.182 88 44 3 178 262 233 317 1.28e-15 77.0
Msa0774220 MtrunA17_Chr8g0383351 52.542 59 25 1 205 263 160 215 1.42e-15 75.5
Msa0774220 MtrunA17_Chr4g0071521 47.059 85 41 3 179 262 219 300 2.07e-15 76.3
Msa0774220 MtrunA17_Chr2g0293481 39.394 99 54 2 168 262 226 322 2.22e-15 77.0
Msa0774220 MtrunA17_Chr3g0079691 51.515 66 26 3 197 262 274 333 3.33e-15 77.0
Msa0774220 MtrunA17_Chr7g0228941 54.237 59 25 1 204 262 51 107 3.96e-15 71.2
Msa0774220 MtrunA17_Chr4g0053281 45.882 85 42 3 178 262 241 321 4.99e-15 75.1
Msa0774220 MtrunA17_Chr7g0263531 49.275 69 29 3 195 262 179 242 6.96e-15 75.5
Msa0774220 MtrunA17_Chr7g0263531 49.123 57 26 1 206 262 362 415 7.80e-11 63.2
Msa0774220 MtrunA17_Chr1g0184691 45.122 82 40 3 194 273 281 359 1.23e-14 74.3
Msa0774220 MtrunA17_Chr2g0315381 43.902 82 45 1 187 268 127 207 1.27e-14 73.9
Msa0774220 MtrunA17_Chr3g0139021 54.237 59 25 1 204 262 313 369 1.27e-14 74.7
Msa0774220 MtrunA17_Chr8g0379461 47.692 65 32 1 198 262 69 131 1.74e-14 72.4
Msa0774220 MtrunA17_Chr5g0443131 54.098 61 25 1 205 265 157 214 2.00e-14 73.2
Msa0774220 MtrunA17_Chr3g0129361 53.448 58 24 1 205 262 144 198 2.03e-14 71.6
Msa0774220 MtrunA17_Chr7g0218081 48.387 62 28 2 201 262 224 281 2.31e-14 74.3
Msa0774220 MtrunA17_Chr7g0218081 48.276 58 27 1 205 262 410 464 7.60e-12 66.6
Msa0774220 MtrunA17_Chr7g0238921 52.542 59 25 1 205 263 124 179 2.79e-14 72.8
Msa0774220 MtrunA17_Chr4g0051881 55.172 58 23 1 205 262 178 232 3.75e-14 72.8
Msa0774220 MtrunA17_Chr5g0428351 45.588 68 37 0 206 273 184 251 4.19e-14 72.4
Msa0774220 MtrunA17_Chr5g0401821 42.353 85 45 3 179 262 173 254 4.71e-14 71.6
Msa0774220 MtrunA17_Chr2g0319961 40.566 106 51 4 174 274 47 145 4.90e-14 71.2
Msa0774220 MtrunA17_Chr8g0386241 50.847 59 26 1 205 263 103 158 5.76e-14 69.7
Msa0774220 MtrunA17_Chr5g0418191 47.059 68 32 2 195 262 141 204 6.79e-14 72.4
Msa0774220 MtrunA17_Chr5g0418191 46.552 58 28 1 205 262 320 374 4.83e-11 63.9
Msa0774220 MtrunA17_Chr8g0335811 53.448 58 24 1 205 262 154 208 8.41e-14 70.1
Msa0774220 MtrunA17_Chr2g0315371 49.206 63 32 0 205 267 141 203 1.23e-13 70.9
Msa0774220 MtrunA17_Chr7g0248721 53.125 64 24 3 205 266 141 200 2.09e-13 68.9
Msa0774220 MtrunA17_Chr7g0249311 50.000 58 25 2 205 262 179 232 5.62e-13 70.1
Msa0774220 MtrunA17_Chr7g0270191 40.404 99 49 3 205 296 145 240 6.28e-13 68.6
Msa0774220 MtrunA17_Chr2g0289181 49.153 59 28 1 204 262 13 69 8.02e-13 67.4
Msa0774220 MtrunA17_Chr5g0445311 48.276 58 27 1 205 262 364 418 8.13e-13 69.3
Msa0774220 MtrunA17_Chr3g0101131 48.276 58 27 1 205 262 415 469 1.04e-12 69.3
Msa0774220 MtrunA17_Chr3g0106491 36.697 109 53 4 195 302 188 281 1.57e-12 68.6
Msa0774220 MtrunA17_Chr3g0106491 46.552 58 28 1 205 262 365 419 5.24e-11 63.9
Msa0774220 MtrunA17_Chr1g0194481 51.724 58 25 1 205 262 158 212 1.58e-12 67.8
Msa0774220 MtrunA17_Chr5g0432161 48.276 58 26 2 205 262 244 297 2.00e-12 68.2
Msa0774220 MtrunA17_Chr5g0432161 48.276 58 27 1 205 262 421 475 6.42e-12 66.6
Msa0774220 MtrunA17_Chr4g0042831 35.106 94 50 2 199 292 139 221 2.99e-12 67.4
Msa0774220 MtrunA17_Chr4g0042831 48.276 58 27 1 205 262 278 332 1.51e-11 65.1
Msa0774220 MtrunA17_Chr2g0322391 53.448 58 25 1 206 263 350 405 3.08e-12 67.8
Msa0774220 MtrunA17_Chr3g0099641 50.877 57 26 1 206 262 252 306 5.37e-12 66.6
Msa0774220 MtrunA17_Chr7g0243891 44.928 69 34 2 194 262 205 269 5.38e-12 67.0
Msa0774220 MtrunA17_Chr4g0004911 44.828 58 29 1 205 262 184 238 5.61e-12 66.6
Msa0774220 MtrunA17_Chr2g0294231 44.737 76 33 2 205 280 203 269 5.89e-12 66.2
Msa0774220 MtrunA17_Chr4g0075201 46.575 73 27 2 206 278 268 328 5.97e-12 66.6
Msa0774220 MtrunA17_Chr1g0150281 49.123 57 27 1 206 262 141 195 6.57e-12 65.5
Msa0774220 MtrunA17_Chr2g0288871 52.632 57 25 1 206 262 306 360 7.18e-12 66.6
Msa0774220 MtrunA17_Chr7g0270831 52.632 57 25 1 206 262 217 271 8.71e-12 66.2
Msa0774220 MtrunA17_Chr8g0387501 52.632 57 25 1 206 262 130 184 1.06e-11 64.7
Msa0774220 MtrunA17_Chr3g0136961 44.615 65 34 1 198 262 131 193 1.49e-11 64.3
Msa0774220 MtrunA17_Chr8g0348311 52.632 57 25 1 206 262 156 210 1.74e-11 64.7
Msa0774220 MtrunA17_Chr4g0074001 47.368 57 27 1 206 262 360 413 2.07e-11 65.1
Msa0774220 MtrunA17_Chr5g0447701 47.368 57 27 1 206 262 253 306 4.08e-11 63.9
Msa0774220 MtrunA17_Chr7g0248691 41.379 87 42 3 176 262 132 209 4.48e-11 63.5
Msa0774220 MtrunA17_Chr1g0202461 49.123 57 27 1 206 262 262 316 5.55e-11 63.5
Msa0774220 MtrunA17_Chr6g0485431 50.000 58 25 2 206 262 155 209 6.08e-11 63.2
Msa0774220 MtrunA17_Chr7g0270051 47.458 59 29 1 204 262 52 108 7.09e-11 62.0
Msa0774220 MtrunA17_Chr4g0060271 52.632 57 25 1 206 262 256 310 7.49e-11 63.5
Msa0774220 MtrunA17_Chr4g0068541 45.763 59 29 1 205 263 208 263 8.88e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0774220 AT2G40740.1 34.659 352 148 11 1 346 11 286 1.52e-51 173
Msa0774220 AT2G40740.3 34.659 352 148 11 1 346 20 295 3.14e-51 173
Msa0774220 AT4G11070.2 52.174 92 42 2 181 271 85 175 2.81e-22 95.5
Msa0774220 AT4G11070.1 52.174 92 42 2 181 271 117 207 4.38e-22 95.5
Msa0774220 AT4G23810.1 59.420 69 28 0 201 269 154 222 6.50e-22 95.5
Msa0774220 AT5G24110.1 45.918 98 52 1 178 275 87 183 2.30e-21 93.6
Msa0774220 AT2G40740.2 42.188 128 42 5 221 346 145 242 4.36e-21 91.7
Msa0774220 AT2G46400.1 36.429 140 80 4 149 288 56 186 6.88e-20 89.0
Msa0774220 AT3G56400.1 52.174 69 33 0 205 273 120 188 3.41e-19 87.0
Msa0774220 AT1G66550.2 53.968 63 29 0 202 264 100 162 2.75e-18 83.6
Msa0774220 AT1G66550.1 53.968 63 29 0 202 264 105 167 3.50e-18 83.6
Msa0774220 AT1G66560.1 43.956 91 44 1 174 264 79 162 6.99e-18 82.4
Msa0774220 AT1G64000.1 56.897 58 22 1 205 262 114 168 6.85e-17 78.6
Msa0774220 AT2G40750.1 53.333 60 28 0 205 264 152 211 2.89e-16 79.3
Msa0774220 AT5G41570.1 56.897 58 22 1 205 262 98 152 3.00e-16 76.3
Msa0774220 AT4G31550.2 44.048 84 44 2 179 262 220 300 3.92e-16 79.0
Msa0774220 AT5G01900.1 54.237 59 27 0 206 264 111 169 4.33e-16 77.8
Msa0774220 AT4G31550.1 48.235 85 40 3 178 262 221 301 5.01e-16 78.6
Msa0774220 AT2G30590.1 32.639 144 89 5 123 262 229 368 8.88e-16 78.2
Msa0774220 AT1G29280.1 44.086 93 41 4 176 262 42 129 1.00e-15 76.6
Msa0774220 AT2G24570.1 46.667 90 44 3 178 267 218 303 1.10e-15 77.4
Msa0774220 AT2G46130.1 55.172 58 23 1 205 262 30 84 1.43e-15 72.4
Msa0774220 AT2G23320.1 41.176 85 46 3 179 262 214 295 1.75e-15 76.6
Msa0774220 AT1G69310.4 49.254 67 30 2 205 271 147 209 2.75e-15 75.9
Msa0774220 AT1G69310.2 49.254 67 30 2 205 271 147 209 2.75e-15 75.9
Msa0774220 AT1G69310.1 49.254 67 30 2 205 271 147 209 2.75e-15 75.9
Msa0774220 AT4G26440.1 48.052 77 27 3 203 279 176 239 3.89e-15 77.0
Msa0774220 AT5G45260.1 31.343 134 88 1 133 262 1132 1265 6.74e-15 76.6
Msa0774220 AT4G18170.1 27.925 265 158 9 76 338 59 292 8.64e-15 74.7
Msa0774220 AT5G28650.1 45.882 85 42 3 179 262 236 317 8.97e-15 74.7
Msa0774220 AT4G39410.1 42.857 84 38 2 205 281 223 303 1.21e-14 74.3
Msa0774220 AT1G66600.1 36.134 119 55 3 146 264 65 162 1.51e-14 72.8
Msa0774220 AT2G34830.2 43.023 86 43 2 181 262 196 279 1.56e-14 74.7
Msa0774220 AT2G34830.1 43.023 86 43 2 181 262 187 270 1.58e-14 74.7
Msa0774220 AT5G22570.1 49.153 59 30 0 206 264 111 169 1.92e-14 73.6
Msa0774220 AT3G04670.1 41.237 97 53 3 179 274 236 329 3.33e-14 73.2
Msa0774220 AT2G37260.1 43.925 107 53 3 173 279 321 420 3.39e-14 73.9
Msa0774220 AT2G37260.1 54.386 57 22 2 206 262 166 218 1.87e-13 71.6
Msa0774220 AT3G58710.1 43.478 92 43 4 176 262 39 126 3.83e-14 72.4
Msa0774220 AT2G37260.2 43.925 107 53 3 173 279 239 338 3.98e-14 73.2
Msa0774220 AT2G37260.2 54.386 57 22 2 206 262 84 136 1.93e-13 71.2
Msa0774220 AT2G37260.3 43.925 107 53 3 173 279 241 340 4.04e-14 73.2
Msa0774220 AT2G37260.3 54.386 57 22 2 206 262 86 138 1.90e-13 71.2
Msa0774220 AT2G44745.1 53.448 58 24 1 205 262 145 199 4.26e-14 71.2
Msa0774220 AT1G29860.1 55.172 58 23 1 205 262 136 190 5.29e-14 72.0
Msa0774220 AT1G80590.1 50.847 59 29 0 206 264 86 144 6.81e-14 70.9
Msa0774220 AT1G30650.1 43.023 86 43 2 181 262 189 272 6.93e-14 72.8
Msa0774220 AT2G03340.1 48.529 68 29 3 196 262 241 303 7.92e-14 72.8
Msa0774220 AT2G03340.1 40.964 83 46 1 205 287 415 494 2.60e-11 65.1
Msa0774220 AT3G01080.3 48.387 62 28 2 201 262 201 258 8.46e-14 72.0
Msa0774220 AT3G58710.2 40.426 94 48 3 174 262 35 125 1.06e-13 70.9
Msa0774220 AT3G01080.2 48.387 62 28 2 201 262 201 258 2.72e-13 71.2
Msa0774220 AT5G46350.1 51.724 58 25 1 205 262 183 237 2.97e-13 70.5
Msa0774220 AT5G13080.1 53.448 58 24 1 205 262 67 121 3.41e-13 67.0
Msa0774220 AT3G01080.1 48.387 62 28 2 201 262 163 220 4.01e-13 70.5
Msa0774220 AT2G38470.1 43.182 88 40 4 181 262 154 237 4.12e-13 70.9
Msa0774220 AT1G13960.1 45.833 72 33 3 192 262 216 282 4.31e-13 70.9
Msa0774220 AT1G13960.1 46.552 58 28 1 205 262 409 463 8.80e-11 63.5
Msa0774220 AT1G13960.2 45.833 72 33 3 192 262 63 129 7.95e-13 69.3
Msa0774220 AT4G24240.1 41.758 91 49 3 173 262 249 336 9.41e-13 68.9
Msa0774220 AT5G52830.1 48.780 82 34 3 181 262 147 220 1.02e-12 68.9
Msa0774220 AT5G26170.1 46.552 58 28 1 205 262 113 167 1.16e-12 66.2
Msa0774220 AT4G31800.2 43.284 67 36 1 196 262 102 166 1.81e-12 67.0
Msa0774220 AT4G26640.7 48.276 58 27 1 205 262 341 395 1.92e-12 68.6
Msa0774220 AT5G56270.1 45.455 77 29 3 203 279 271 334 1.94e-12 68.9
Msa0774220 AT5G49520.1 50.000 58 26 1 205 262 221 275 2.00e-12 68.2
Msa0774220 AT4G04450.2 42.045 88 38 3 206 293 199 273 2.01e-12 68.6
Msa0774220 AT4G26640.2 48.276 58 27 1 205 262 381 435 2.16e-12 68.6
Msa0774220 AT4G23550.1 52.632 57 25 1 206 262 141 195 2.17e-12 67.4
Msa0774220 AT4G26640.5 48.276 58 27 1 205 262 205 259 2.20e-12 68.2
Msa0774220 AT4G26640.6 48.276 58 27 1 205 262 205 259 2.20e-12 68.2
Msa0774220 AT4G26640.4 48.276 58 27 1 205 262 205 259 2.20e-12 68.2
Msa0774220 AT4G26640.3 48.276 58 27 1 205 262 205 259 2.20e-12 68.2
Msa0774220 AT4G26640.1 48.276 58 27 1 205 262 309 363 2.28e-12 68.6
Msa0774220 AT4G31800.1 43.284 67 36 1 196 262 167 231 2.41e-12 67.4
Msa0774220 AT4G04450.1 42.045 88 38 3 206 293 293 367 2.95e-12 68.2
Msa0774220 AT4G30935.1 42.857 77 40 2 206 282 332 404 3.34e-12 67.8
Msa0774220 AT2G47260.1 38.462 91 53 1 180 270 149 236 7.60e-12 66.2
Msa0774220 AT4G01250.1 38.554 83 44 2 180 262 108 183 1.11e-11 65.5
Msa0774220 AT1G62300.1 52.632 57 25 1 206 262 313 367 1.78e-11 65.9
Msa0774220 AT4G01720.1 52.632 57 25 1 206 262 240 294 2.50e-11 65.1
Msa0774220 AT2G30250.1 52.632 57 24 1 206 262 329 382 3.06e-11 64.7
Msa0774220 AT5G43290.1 49.153 59 27 1 205 263 114 169 3.42e-11 63.5
Msa0774220 AT5G45050.1 42.353 85 42 2 178 262 1158 1235 4.06e-11 65.1
Msa0774220 AT4G22070.4 52.632 57 25 1 206 262 298 352 4.16e-11 64.7
Msa0774220 AT4G22070.3 52.632 57 25 1 206 262 298 352 4.16e-11 64.7
Msa0774220 AT4G22070.2 52.632 57 25 1 206 262 298 352 4.16e-11 64.7
Msa0774220 AT4G22070.1 52.632 57 25 1 206 262 298 352 4.16e-11 64.7
Msa0774220 AT5G45050.2 42.353 85 42 2 178 262 1130 1207 4.19e-11 65.1
Msa0774220 AT1G55600.1 44.776 67 34 1 196 262 298 361 4.26e-11 64.7
Msa0774220 AT5G45050.4 42.353 85 42 2 178 262 1019 1096 4.50e-11 65.1
Msa0774220 AT2G04880.2 47.368 57 27 1 206 262 284 337 4.78e-11 64.3
Msa0774220 AT2G04880.2 51.724 58 24 2 205 262 111 164 9.29e-11 63.5
Msa0774220 AT2G04880.1 47.368 57 27 1 206 262 308 361 4.85e-11 64.3
Msa0774220 AT2G04880.1 51.724 58 24 2 205 262 111 164 8.31e-11 63.5
Msa0774220 AT2G25000.2 37.500 80 48 1 183 262 81 158 5.60e-11 62.4
Msa0774220 AT2G25000.4 37.500 80 48 1 183 262 81 158 5.60e-11 62.4
Msa0774220 AT2G25000.3 37.500 80 48 1 183 262 103 180 9.13e-11 62.0

Find 94 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5_2:-50158294 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5_2:-50161127 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5_2:-50161159 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.259697 5_2:-50158346 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5_2:+50158675 Msa0774220:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5_2:+50160390 Msa0774220:CDS
TGAAATGGGTGGGAGGGATA+TGG 0.320333 5_2:+50158644 Msa0774220:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5_2:+50161368 Msa0774220:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.332908 5_2:+50158629 Msa0774220:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5_2:+50158456 Msa0774220:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5_2:-50161287 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5_2:-50161123 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5_2:-50158277 None:intergenic
CCTTTATCAGAAGTTACTAT+AGG 0.370143 5_2:+50161073 Msa0774220:intron
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5_2:+50161413 Msa0774220:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5_2:-50160381 None:intergenic
ATTAGAACACTCACATCAAA+AGG 0.383819 5_2:-50158543 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.384626 5_2:+50158604 Msa0774220:CDS
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5_2:-50158408 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5_2:+50158666 Msa0774220:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5_2:+50158497 Msa0774220:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5_2:+50160305 Msa0774220:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5_2:+50160379 Msa0774220:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5_2:-50158276 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5_2:+50161356 Msa0774220:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5_2:+50161301 Msa0774220:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5_2:-50160348 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5_2:-50161265 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5_2:-50161158 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5_2:+50158465 Msa0774220:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5_2:-50161286 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5_2:-50161240 None:intergenic
GAACATTACATCGGCCATAT+CGG 0.452060 5_2:-50161382 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5_2:-50161231 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.454574 5_2:+50158630 Msa0774220:CDS
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5_2:-50161277 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5_2:-50158208 None:intergenic
GGTGGTGGTGGATATGATGA+TGG 0.483740 5_2:-50161219 None:intergenic
TAAGGTCTAGGGAAACTTTG+AGG 0.489231 5_2:+50158616 Msa0774220:CDS
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5_2:-50161300 None:intergenic
CGTTCAGTTGTTAAGGTCTA+GGG 0.489911 5_2:+50158605 Msa0774220:CDS
GGACCCTCTACTTCAAGATT+CGG 0.492644 5_2:+50161342 Msa0774220:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5_2:-50161311 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5_2:+50161318 Msa0774220:CDS
TCTAGGGAAACTTTGAGGAT+TGG 0.504126 5_2:+50158621 Msa0774220:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5_2:+50160342 Msa0774220:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5_2:+50158598 Msa0774220:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5_2:+50161312 Msa0774220:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5_2:+50161284 Msa0774220:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5_2:-50158293 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5_2:+50161165 Msa0774220:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5_2:-50160354 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5_2:+50158459 Msa0774220:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5_2:+50160369 Msa0774220:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5_2:-50158286 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5_2:+50158576 Msa0774220:CDS
AAGGTTTAGATAGATCAAAA+GGG 0.540645 5_2:+50158667 Msa0774220:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5_2:+50158564 Msa0774220:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5_2:+50161270 Msa0774220:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5_2:-50161245 None:intergenic
TGCGGTGAACCACGACCTCA+GGG 0.552440 5_2:+50161302 Msa0774220:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5_2:-50161297 None:intergenic
TACTTGGCCGCAAGCACCCA+AGG 0.555851 5_2:+50160424 Msa0774220:CDS
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5_2:-50161237 None:intergenic
TAATTTACCTTGGGTGCTTG+CGG 0.559902 5_2:-50160431 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5_2:+50158633 Msa0774220:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5_2:+50161321 Msa0774220:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5_2:+50158634 Msa0774220:CDS
GGCCGATGTAATGTTCAACT+CGG 0.582893 5_2:+50161389 Msa0774220:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5_2:+50161271 Msa0774220:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5_2:+50158637 Msa0774220:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5_2:-50161345 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5_2:-50158542 None:intergenic
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5_2:+50161274 Msa0774220:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5_2:+50161402 Msa0774220:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.607995 5_2:+50158638 Msa0774220:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5_2:-50158418 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5_2:+50161458 Msa0774220:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5_2:-50161234 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5_2:+50161390 Msa0774220:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5_2:-50161346 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5_2:-50160378 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5_2:+50158567 Msa0774220:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5_2:+50158570 Msa0774220:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5_2:-50161375 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5_2:+50161306 Msa0774220:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5_2:+50158579 Msa0774220:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5_2:+50158263 Msa0774220:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5_2:+50161359 Msa0774220:CDS
ATGGGTGGGAGGGATATGGA+AGG 0.678067 5_2:+50158648 Msa0774220:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5_2:-50161317 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5_2:-50161391 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5_2:+50161303 Msa0774220:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5_2:-50158290 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAATCAATTTTTTTTTTA+TGG - chr5_2:50159829-50159848 None:intergenic 10.0%
!!! AAAATTGATTTGAATTTTTT+TGG + chr5_2:50159836-50159855 Msa0774220:intron 10.0%
!!! TTTTACATAAAATTATAAGT+AGG + chr5_2:50160206-50160225 Msa0774220:intron 10.0%
!! TAAATTATTTAATATCAAAC+TGG + chr5_2:50161008-50161027 Msa0774220:intron 10.0%
!!! GTTTTTATTATATAATTTGT+TGG + chr5_2:50161030-50161049 Msa0774220:intron 10.0%
!!! TTATATACATACCATTTTTT+TGG - chr5_2:50158738-50158757 None:intergenic 15.0%
!! TCAAAGTTTCATATAATAAA+TGG + chr5_2:50158820-50158839 Msa0774220:intron 15.0%
!!! TCACTAGTTAATTATTTTAA+TGG + chr5_2:50160015-50160034 Msa0774220:intron 15.0%
!!! AGTAAAGTGAATTTTTTTTT+TGG - chr5_2:50160119-50160138 None:intergenic 15.0%
!! AATTATTGTTAATTTACCTT+GGG - chr5_2:50160443-50160462 None:intergenic 15.0%
!! TAATTATTGTTAATTTACCT+TGG - chr5_2:50160444-50160463 None:intergenic 15.0%
!! AGAATTGTACTTTATAATAT+AGG + chr5_2:50160467-50160486 Msa0774220:intron 15.0%
!! GAATTGTACTTTATAATATA+GGG + chr5_2:50160468-50160487 Msa0774220:intron 15.0%
!! AAATATTTAACAAGTAATCA+AGG - chr5_2:50160790-50160809 None:intergenic 15.0%
!!! ATTTCTAAGGTATTGAATTT+AGG + chr5_2:50158929-50158948 Msa0774220:intron 20.0%
!! AAAAATCTTATGGAAATGAT+TGG - chr5_2:50159330-50159349 None:intergenic 20.0%
!! TTTCTTTCAATTATTGTTGT+TGG - chr5_2:50159550-50159569 None:intergenic 20.0%
!! AAATTTATATATGCAAGATC+CGG + chr5_2:50160066-50160085 Msa0774220:intron 20.0%
!! AATAGTTTGACACAATTATT+AGG + chr5_2:50160143-50160162 Msa0774220:intron 20.0%
!! CAATTATTAGGAAGTTAATA+TGG + chr5_2:50160155-50160174 Msa0774220:intron 20.0%
!! AAGAAATATTCTTAGAAGAA+AGG - chr5_2:50160576-50160595 None:intergenic 20.0%
!!! TTTCTTCTAAGAATATTTCT+TGG + chr5_2:50160575-50160594 Msa0774220:intron 20.0%
!!! TTTTATCTAACTGAAGATAA+AGG + chr5_2:50161444-50161463 Msa0774220:CDS 20.0%
! ATTGAGAATAAGATGATAAG+TGG + chr5_2:50158564-50158583 Msa0774220:CDS 25.0%
! AAGGTTTAGATAGATCAAAA+GGG + chr5_2:50158667-50158686 Msa0774220:CDS 25.0%
! ATTCAATAACATAGATTGTG+AGG - chr5_2:50158858-50158877 None:intergenic 25.0%
! ATGTTATTGAATCAAAGTCT+TGG + chr5_2:50158866-50158885 Msa0774220:intron 25.0%
!!! CAACACAAAGATTATTTCTA+AGG + chr5_2:50158916-50158935 Msa0774220:intron 25.0%
!!! CATATTTGGGTTTTAGTTTA+TGG + chr5_2:50159009-50159028 Msa0774220:intron 25.0%
! TTGCAATTCAACTGAAATTA+AGG - chr5_2:50159099-50159118 None:intergenic 25.0%
! ATACATCAAAGTTGAAAGTT+AGG - chr5_2:50159171-50159190 None:intergenic 25.0%
!! TAGGGTGAAAATTTTCATTA+TGG - chr5_2:50159267-50159286 None:intergenic 25.0%
! TAATGTGAAAATCCTAAGTA+GGG - chr5_2:50159285-50159304 None:intergenic 25.0%
! ATAATGTGAAAATCCTAAGT+AGG - chr5_2:50159286-50159305 None:intergenic 25.0%
!! TTCCATAAGATTTTTACTAG+AGG + chr5_2:50159335-50159354 Msa0774220:intron 25.0%
!! TCCATAAGATTTTTACTAGA+GGG + chr5_2:50159336-50159355 Msa0774220:intron 25.0%
! TAAAAGTAGAGATTGAAATG+AGG + chr5_2:50159392-50159411 Msa0774220:intron 25.0%
!! ATGTAAGATAGAATTTTCCA+TGG + chr5_2:50159670-50159689 Msa0774220:intron 25.0%
!!! TTTTTTGGTGTTTGCTATTT+TGG + chr5_2:50159851-50159870 Msa0774220:intron 25.0%
! AATTTATATATGCAAGATCC+GGG + chr5_2:50160067-50160086 Msa0774220:intron 25.0%
!!! ATTTTTTTTTTGGTGATGAC+CGG - chr5_2:50160109-50160128 None:intergenic 25.0%
! GTATAATTCAGTTGTTAGAT+TGG + chr5_2:50160177-50160196 Msa0774220:intron 25.0%
! TGAAACTTTCAAACTCTTTA+TGG - chr5_2:50160503-50160522 None:intergenic 25.0%
!! ATTCTTAGAAGAAAGGTATA+AGG - chr5_2:50160569-50160588 None:intergenic 25.0%
!! CTGGACACAAATATAATTTT+TGG - chr5_2:50161107-50161126 None:intergenic 25.0%
!! ATGTTACTTCAACTATGTTA+GGG - chr5_2:50161161-50161180 None:intergenic 25.0%
!! TATGTTACTTCAACTATGTT+AGG - chr5_2:50161162-50161181 None:intergenic 25.0%
! ATAGTTGAAGTAACATATAG+AGG + chr5_2:50161165-50161184 Msa0774220:CDS 25.0%
!!! TGCTTTTTCTACATCATCAA+TGG - chr5_2:50158316-50158335 None:intergenic 30.0%
CATCATCATTACTCTTTCTT+TGG - chr5_2:50158349-50158368 None:intergenic 30.0%
ATTTGAGCAAAGGAATTAGA+AGG - chr5_2:50158411-50158430 None:intergenic 30.0%
AAAACTCAACATGCTCAAAT+TGG + chr5_2:50158456-50158475 Msa0774220:CDS 30.0%
TTAGAACACTCACATCAAAA+GGG - chr5_2:50158545-50158564 None:intergenic 30.0%
ATTAGAACACTCACATCAAA+AGG - chr5_2:50158546-50158565 None:intergenic 30.0%
GAAGGTTTAGATAGATCAAA+AGG + chr5_2:50158666-50158685 Msa0774220:CDS 30.0%
!!! TACCATTTTTTTGGTCTTGA+AGG - chr5_2:50158729-50158748 None:intergenic 30.0%
ACCGGGTAATTTACATATTT+GGG + chr5_2:50158996-50159015 Msa0774220:intron 30.0%
ACCCAAATATGTAAATTACC+CGG - chr5_2:50159000-50159019 None:intergenic 30.0%
!!! GGGTTTTAGTTTATGGTTAT+AGG + chr5_2:50159016-50159035 Msa0774220:intron 30.0%
! TTCTGTAAGATTTTCACTAG+AGG + chr5_2:50159237-50159256 Msa0774220:intron 30.0%
! TCTGTAAGATTTTCACTAGA+GGG + chr5_2:50159238-50159257 Msa0774220:intron 30.0%
! TGAAAATTTTCACCCTACTT+AGG + chr5_2:50159270-50159289 Msa0774220:intron 30.0%
TCCCTCTAGTAAAAATCTTA+TGG - chr5_2:50159340-50159359 None:intergenic 30.0%
GATTACTCAATGTTTGTACT+AGG - chr5_2:50159372-50159391 None:intergenic 30.0%
AGATTGAAATGAGGTTAACT+TGG + chr5_2:50159401-50159420 Msa0774220:intron 30.0%
! AGGTCACTAGTTTATGAAAA+TGG + chr5_2:50159467-50159486 Msa0774220:intron 30.0%
TCTACAATTGTGACAAAAAC+AGG - chr5_2:50159509-50159528 None:intergenic 30.0%
! CTTTCAATTATTGTTGTTGG+CGG - chr5_2:50159547-50159566 None:intergenic 30.0%
! ACAAAAGTGAAATGCTTTTG+AGG + chr5_2:50159574-50159593 Msa0774220:intron 30.0%
! GGTATTGAAAGCTTTAGTTT+AGG + chr5_2:50159595-50159614 Msa0774220:intron 30.0%
AACACACCAATAATAACGTA+CGG - chr5_2:50159623-50159642 None:intergenic 30.0%
CTAATTACTAAGAAAGACCA+TGG - chr5_2:50159690-50159709 None:intergenic 30.0%
CATGGTCTTTCTTAGTAATT+AGG + chr5_2:50159688-50159707 Msa0774220:intron 30.0%
TAGTTTACATGACCAAAACA+AGG + chr5_2:50159761-50159780 Msa0774220:intron 30.0%
! AGGTCTTTGCTATAGTATTT+AGG + chr5_2:50159781-50159800 Msa0774220:intron 30.0%
ATTTATATATGCAAGATCCG+GGG + chr5_2:50160068-50160087 Msa0774220:intron 30.0%
!!! TTTTTTTTTTGGTGATGACC+GGG - chr5_2:50160108-50160127 None:intergenic 30.0%
TATGGCCAAAAAGAAATACT+TGG + chr5_2:50160408-50160427 Msa0774220:CDS 30.0%
!! AAGAAAGGTATAAGGTTTTC+TGG - chr5_2:50160561-50160580 None:intergenic 30.0%
TGTGAATGAGTCAATACATA+GGG - chr5_2:50160636-50160655 None:intergenic 30.0%
CCTATAGTAACTTCTGATAA+AGG - chr5_2:50161076-50161095 None:intergenic 30.0%
CCTTTATCAGAAGTTACTAT+AGG + chr5_2:50161073-50161092 Msa0774220:intron 30.0%
TGGATGAGAATAGCATTTCA+GGG - chr5_2:50158296-50158315 None:intergenic 35.0%
ATGGATGAGAATAGCATTTC+AGG - chr5_2:50158297-50158316 None:intergenic 35.0%
ATGAGACAGAATTTGAGCAA+AGG - chr5_2:50158421-50158440 None:intergenic 35.0%
TTCAATGCATGCACATTCAA+TGG + chr5_2:50158497-50158516 Msa0774220:CDS 35.0%
GAGAATAAGATGATAAGTGG+CGG + chr5_2:50158567-50158586 Msa0774220:CDS 35.0%
! AACTTTGAGGATTGGTGAAA+TGG + chr5_2:50158629-50158648 Msa0774220:CDS 35.0%
! ACTTTGAGGATTGGTGAAAT+GGG + chr5_2:50158630-50158649 Msa0774220:CDS 35.0%
GATAGATCAAAAGGGTTAGA+AGG + chr5_2:50158675-50158694 Msa0774220:CDS 35.0%
!!! CATTTTTTTGGTCTTGAAGG+TGG - chr5_2:50158726-50158745 None:intergenic 35.0%
CACCTTCAAGACCAAAAAAA+TGG + chr5_2:50158724-50158743 Msa0774220:CDS 35.0%
! GGTATTGAATTTAGGTGTTG+AGG + chr5_2:50158937-50158956 Msa0774220:intron 35.0%
! TTTCAGTGTTTTTCCAACAC+CGG + chr5_2:50158978-50158997 Msa0774220:intron 35.0%
ATATGTAAATTACCCGGTGT+TGG - chr5_2:50158994-50159013 None:intergenic 35.0%
CACCGGGTAATTTACATATT+TGG + chr5_2:50158995-50159014 Msa0774220:intron 35.0%
TTATAGGTGCCTTGCTAATT+GGG + chr5_2:50159032-50159051 Msa0774220:intron 35.0%
TTGCGTTAAGATGTGACATA+TGG + chr5_2:50159071-50159090 Msa0774220:intron 35.0%
TGCGTTAAGATGTGACATAT+GGG + chr5_2:50159072-50159091 Msa0774220:intron 35.0%
ATGTGTGTGTGATTGAATCT+AGG + chr5_2:50159431-50159450 Msa0774220:intron 35.0%
AACTAGTGACCTAATTAGCA+AGG - chr5_2:50159459-50159478 None:intergenic 35.0%
TAGGATCCGTACGTTATTAT+TGG + chr5_2:50159614-50159633 Msa0774220:intron 35.0%
TGGTGTGTTTGTAAACAGAA+AGG + chr5_2:50159634-50159653 Msa0774220:intron 35.0%
AATTAGGCAACAAGTCATCA+TGG + chr5_2:50159704-50159723 Msa0774220:intron 35.0%
!! CTATAGCAAAGACCTTGTTT+TGG - chr5_2:50159776-50159795 None:intergenic 35.0%
GTGTCTAAGAACTGTGTATT+TGG + chr5_2:50159877-50159896 Msa0774220:intron 35.0%
!!! TTTTTTTTTGGTGATGACCG+GGG - chr5_2:50160107-50160126 None:intergenic 35.0%
TCACACACAAACACACATAT+AGG - chr5_2:50160247-50160266 None:intergenic 35.0%
! ATGTGAAGGCACAACAAATT+AGG - chr5_2:50160278-50160297 None:intergenic 35.0%
GGCTTTACTTGGAGAAAATA+TGG + chr5_2:50160390-50160409 Msa0774220:CDS 35.0%
! AAGGTATAAGGTTTTCTGGA+TGG - chr5_2:50160557-50160576 None:intergenic 35.0%
GTGTGAATGAGTCAATACAT+AGG - chr5_2:50160637-50160656 None:intergenic 35.0%
CTAATGTTCACAAGTAGGTT+TGG - chr5_2:50161243-50161262 None:intergenic 35.0%
TGGTACTAATGTTCACAAGT+AGG - chr5_2:50161248-50161267 None:intergenic 35.0%
!! AAGATTCGGCATTGATTATC+CGG + chr5_2:50161356-50161375 Msa0774220:CDS 35.0%
TGAACTAGAACTACCCAACA+TGG + chr5_2:50158263-50158282 Msa0774220:CDS 40.0%
ATGAGAATAGCATTTCAGGG+TGG - chr5_2:50158293-50158312 None:intergenic 40.0%
ACTCAACATGCTCAAATTGG+TGG + chr5_2:50158459-50158478 Msa0774220:CDS 40.0%
AATAAGATGATAAGTGGCGG+TGG + chr5_2:50158570-50158589 Msa0774220:CDS 40.0%
ACGTTCAGTTGTTAAGGTCT+AGG + chr5_2:50158604-50158623 Msa0774220:CDS 40.0%
CGTTCAGTTGTTAAGGTCTA+GGG + chr5_2:50158605-50158624 Msa0774220:CDS 40.0%
TAAGGTCTAGGGAAACTTTG+AGG + chr5_2:50158616-50158635 Msa0774220:CDS 40.0%
! TCTAGGGAAACTTTGAGGAT+TGG + chr5_2:50158621-50158640 Msa0774220:CDS 40.0%
! TTCAGTGTTTTTCCAACACC+GGG + chr5_2:50158979-50158998 Msa0774220:intron 40.0%
GTTATAGGTGCCTTGCTAAT+TGG + chr5_2:50159031-50159050 Msa0774220:intron 40.0%
ATCTAGTTGCCCAATTAGCA+AGG - chr5_2:50159044-50159063 None:intergenic 40.0%
CAAAGTTGAAAGTTAGGTCG+TGG - chr5_2:50159165-50159184 None:intergenic 40.0%
TCAATCACACACACATTGTG+AGG - chr5_2:50159427-50159446 None:intergenic 40.0%
ATCTAGGTGCCTTGCTAATT+AGG + chr5_2:50159447-50159466 Msa0774220:intron 40.0%
TAGCAGCAGGAAGAATGATT+TGG + chr5_2:50160305-50160324 Msa0774220:intron 40.0%
ATCTCAGTGTTTCCAATCTG+AGG - chr5_2:50160357-50160376 None:intergenic 40.0%
! TGCGGCCAAGTATTTCTTTT+TGG - chr5_2:50160416-50160435 None:intergenic 40.0%
TAATTTACCTTGGGTGCTTG+CGG - chr5_2:50160434-50160453 None:intergenic 40.0%
AAGTCTTTGCACTTGCTTCT+TGG - chr5_2:50161130-50161149 None:intergenic 40.0%
ATGTTCACAAGTAGGTTTGG+TGG - chr5_2:50161240-50161259 None:intergenic 40.0%
GGAAGAAATGGCAGTTTCAA+TGG - chr5_2:50161268-50161287 None:intergenic 40.0%
! AAACTGCCATTTCTTCCCAA+GGG + chr5_2:50161271-50161290 Msa0774220:CDS 40.0%
ATGCCGAATCTTGAAGTAGA+GGG - chr5_2:50161348-50161367 None:intergenic 40.0%
AATGCCGAATCTTGAAGTAG+AGG - chr5_2:50161349-50161368 None:intergenic 40.0%
GAACATTACATCGGCCATAT+CGG - chr5_2:50161385-50161404 None:intergenic 40.0%
! ACCCGAGTTGAACATTACAT+CGG - chr5_2:50161394-50161413 None:intergenic 40.0%
TAGTAGCAGTGGCAATAGTA+TGG + chr5_2:50161413-50161432 Msa0774220:CDS 40.0%
AGATAAAGGTGAACCTGATG+AGG + chr5_2:50161458-50161477 Msa0774220:CDS 40.0%
GAATAGCATTTCAGGGTGGT+TGG - chr5_2:50158289-50158308 None:intergenic 45.0%
CATGCTCAAATTGGTGGAAG+TGG + chr5_2:50158465-50158484 Msa0774220:CDS 45.0%
! TTGAGGATTGGTGAAATGGG+TGG + chr5_2:50158633-50158652 Msa0774220:CDS 45.0%
! TGAGGATTGGTGAAATGGGT+GGG + chr5_2:50158634-50158653 Msa0774220:CDS 45.0%
GCTGTCTTGTGTGATTGATG+AGG - chr5_2:50159736-50159755 None:intergenic 45.0%
TGTTGTGCCTTCACATGCAT+TGG + chr5_2:50160282-50160301 Msa0774220:intron 45.0%
CAAGTAAAGCCATCTTCTGG+TGG - chr5_2:50160381-50160400 None:intergenic 45.0%
! CACCAGAAGATGGCTTTACT+TGG + chr5_2:50160379-50160398 Msa0774220:CDS 45.0%
CTCCAAGTAAAGCCATCTTC+TGG - chr5_2:50160384-50160403 None:intergenic 45.0%
!! TTCACAAGTAGGTTTGGTGG+TGG - chr5_2:50161237-50161256 None:intergenic 45.0%
! GAAACTGCCATTTCTTCCCA+AGG + chr5_2:50161270-50161289 Msa0774220:CDS 45.0%
GGACCCTCTACTTCAAGATT+CGG + chr5_2:50161342-50161361 Msa0774220:CDS 45.0%
!! ATTCGGCATTGATTATCCGG+TGG + chr5_2:50161359-50161378 Msa0774220:CDS 45.0%
GGCCGATGTAATGTTCAACT+CGG + chr5_2:50161389-50161408 Msa0774220:CDS 45.0%
GCCGATGTAATGTTCAACTC+GGG + chr5_2:50161390-50161409 Msa0774220:CDS 45.0%
ATGATAAGTGGCGGTGGTGA+TGG + chr5_2:50158576-50158595 Msa0774220:CDS 50.0%
GCGGTGACGTTCAGTTGTTA+AGG + chr5_2:50158598-50158617 Msa0774220:CDS 50.0%
GATTGGTGAAATGGGTGGGA+GGG + chr5_2:50158638-50158657 Msa0774220:CDS 50.0%
TGAAATGGGTGGGAGGGATA+TGG + chr5_2:50158644-50158663 Msa0774220:CDS 50.0%
GCTGCTACCAATGCATGTGA+AGG - chr5_2:50160292-50160311 None:intergenic 50.0%
! TCACATGCATTGGTAGCAGC+AGG + chr5_2:50160292-50160311 Msa0774220:intron 50.0%
ATGCATCCAGCACCTCAGAT+TGG + chr5_2:50160342-50160361 Msa0774220:CDS 50.0%
! GTGTTTCCAATCTGAGGTGC+TGG - chr5_2:50160351-50160370 None:intergenic 50.0%
ACTGAGATGCCACCAGAAGA+TGG + chr5_2:50160369-50160388 Msa0774220:CDS 50.0%
CTTTGCACTTGCTTCTTGGC+TGG - chr5_2:50161126-50161145 None:intergenic 50.0%
!! GGTGGTGGTGGATATGATGA+TGG - chr5_2:50161222-50161241 None:intergenic 50.0%
!! ACAAGTAGGTTTGGTGGTGG+TGG - chr5_2:50161234-50161253 None:intergenic 50.0%
TGATTATCCGGTGGCCGATA+TGG + chr5_2:50161368-50161387 Msa0774220:CDS 50.0%
TTCAACTCGGGTAGTAGCAG+TGG + chr5_2:50161402-50161421 Msa0774220:CDS 50.0%
! TCAGGGTGGTTGGCCATGTT+GGG - chr5_2:50158279-50158298 None:intergenic 55.0%
! TTCAGGGTGGTTGGCCATGT+TGG - chr5_2:50158280-50158299 None:intergenic 55.0%
!! ATAAGTGGCGGTGGTGATGG+CGG + chr5_2:50158579-50158598 Msa0774220:CDS 55.0%
! GGATTGGTGAAATGGGTGGG+AGG + chr5_2:50158637-50158656 Msa0774220:CDS 55.0%
ATGGGTGGGAGGGATATGGA+AGG + chr5_2:50158648-50158667 Msa0774220:CDS 55.0%
! CTGCCATTTCTTCCCAAGGG+TGG + chr5_2:50161274-50161293 Msa0774220:CDS 55.0%
TGGCCACCCTTGGGAAGAAA+TGG - chr5_2:50161280-50161299 None:intergenic 55.0%
GGGGTTCGAGGTTTGAACCC+CGG - chr5_2:50160088-50160107 None:intergenic 60.0%
GGGGTTCAAACCTCGAACCC+CGG + chr5_2:50160087-50160106 Msa0774220:intron 60.0%
!! TTGGTGATGACCGGGGTTCG+AGG - chr5_2:50160100-50160119 None:intergenic 60.0%
TACTTGGCCGCAAGCACCCA+AGG + chr5_2:50160424-50160443 Msa0774220:CDS 60.0%
TGCGGTGAACCACGACCTCA+GGG + chr5_2:50161302-50161321 Msa0774220:CDS 60.0%
ACATCGGCCATATCGGCCAC+CGG - chr5_2:50161378-50161397 None:intergenic 60.0%
TTCCCAAGGGTGGCCACCTG+CGG + chr5_2:50161284-50161303 Msa0774220:CDS 65.0%
AGGTCGTGGTTCACCGCAGG+TGG - chr5_2:50161300-50161319 None:intergenic 65.0%
CTGAGGTCGTGGTTCACCGC+AGG - chr5_2:50161303-50161322 None:intergenic 65.0%
CTGCGGTGAACCACGACCTC+AGG + chr5_2:50161301-50161320 Msa0774220:CDS 65.0%
GCGGTGAACCACGACCTCAG+GGG + chr5_2:50161303-50161322 Msa0774220:CDS 65.0%
GTGAACCACGACCTCAGGGG+CGG + chr5_2:50161306-50161325 Msa0774220:CDS 65.0%
CACCGCAGGTGGCCACCCTT+GGG - chr5_2:50161289-50161308 None:intergenic 70.0%
TCACCGCAGGTGGCCACCCT+TGG - chr5_2:50161290-50161309 None:intergenic 70.0%
CACGACCTCAGGGGCGGAGA+CGG + chr5_2:50161312-50161331 Msa0774220:CDS 70.0%
CAGTACCGTCTCCGCCCCTG+AGG - chr5_2:50161320-50161339 None:intergenic 70.0%
CTCAGGGGCGGAGACGGTAC+TGG + chr5_2:50161318-50161337 Msa0774220:CDS 70.0%
! AGGGGCGGAGACGGTACTGG+TGG + chr5_2:50161321-50161340 Msa0774220:CDS 70.0%
CGTCTCCGCCCCTGAGGTCG+TGG - chr5_2:50161314-50161333 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 50158210 50161491 50158210 ID=Msa0774220;Name=Msa0774220
chr5_2 mRNA 50158210 50161491 50158210 ID=Msa0774220-mRNA-1;Parent=Msa0774220;Name=Msa0774220-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|360
chr5_2 exon 50158210 50158745 50158210 ID=Msa0774220-mRNA-1:exon:14935;Parent=Msa0774220-mRNA-1
chr5_2 exon 50160308 50160445 50160308 ID=Msa0774220-mRNA-1:exon:14936;Parent=Msa0774220-mRNA-1
chr5_2 exon 50161083 50161491 50161083 ID=Msa0774220-mRNA-1:exon:14937;Parent=Msa0774220-mRNA-1
chr5_2 CDS 50158210 50158745 50158210 ID=Msa0774220-mRNA-1:cds;Parent=Msa0774220-mRNA-1
chr5_2 CDS 50160308 50160445 50160308 ID=Msa0774220-mRNA-1:cds;Parent=Msa0774220-mRNA-1
chr5_2 CDS 50161083 50161491 50161083 ID=Msa0774220-mRNA-1:cds;Parent=Msa0774220-mRNA-1
Gene Sequence

>Msa0774220

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGTTGTCATCACAACATGACACAACAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGAAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATATGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGCACCCAAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCTTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>Msa0774220

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMLSSQHDTTTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRETLRIGEMGGRDMEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKHPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN*