AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0851950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0851950 MtrunA17_Chr5g0428331 89.189 185 14 3 1 184 1 180 2.51e-111 322
Msa0851950 MtrunA17_Chr5g0431811 55.652 115 49 2 165 277 629 743 8.52e-33 129
Msa0851950 MtrunA17_Chr7g0275531 40.299 134 71 2 199 331 111 236 1.23e-23 99.8
Msa0851950 MtrunA17_Chr3g0126391 52.688 93 42 2 175 265 100 192 3.18e-23 99.0
Msa0851950 MtrunA17_Chr8g0387981 58.108 74 31 0 202 275 128 201 7.36e-23 98.2
Msa0851950 MtrunA17_Chr8g0347251 59.091 66 27 0 206 271 136 201 7.12e-21 92.4
Msa0851950 MtrunA17_Chr7g0244981 41.667 108 57 2 163 265 75 181 6.90e-19 85.9
Msa0851950 MtrunA17_Chr7g0244971 37.963 108 62 1 163 265 75 182 3.09e-18 84.0
Msa0851950 MtrunA17_Chr8g0334541 53.731 67 31 0 201 267 98 164 4.81e-18 83.2
Msa0851950 MtrunA17_Chr3g0128671 36.975 119 60 3 162 272 82 193 1.08e-17 82.8
Msa0851950 MtrunA17_Chr2g0284321 43.373 83 45 1 181 263 10 90 1.98e-17 80.1
Msa0851950 MtrunA17_Chr8g0350621 49.231 65 33 0 201 265 106 170 4.94e-17 80.1
Msa0851950 MtrunA17_Chr5g0418781 42.857 105 53 4 171 274 90 188 6.45e-17 80.1
Msa0851950 MtrunA17_Chr1g0147881 44.318 88 43 3 179 263 233 317 2.21e-16 79.3
Msa0851950 MtrunA17_Chr2g0315361 55.556 63 28 0 206 268 148 210 2.27e-16 79.0
Msa0851950 MtrunA17_Chr7g0224801 56.897 58 22 1 206 263 93 147 3.61e-16 75.5
Msa0851950 MtrunA17_Chr4g0071521 48.235 85 40 3 180 263 219 300 4.34e-16 78.2
Msa0851950 MtrunA17_Chr2g0293481 40.816 98 52 2 170 263 227 322 5.30e-16 79.0
Msa0851950 MtrunA17_Chr3g0079691 55.000 60 23 2 204 263 278 333 6.06e-16 79.3
Msa0851950 MtrunA17_Chr7g0263531 50.725 69 28 3 196 263 179 242 1.67e-15 77.4
Msa0851950 MtrunA17_Chr7g0263531 50.877 57 25 1 207 263 362 415 1.84e-11 65.1
Msa0851950 MtrunA17_Chr2g0315381 45.122 82 44 1 188 269 127 207 2.99e-15 75.5
Msa0851950 MtrunA17_Chr5g0443131 55.738 61 24 1 206 266 157 214 3.69e-15 75.5
Msa0851950 MtrunA17_Chr8g0379461 49.231 65 31 1 199 263 69 131 3.76e-15 74.3
Msa0851950 MtrunA17_Chr7g0218081 50.000 62 27 2 202 263 224 281 4.97e-15 76.3
Msa0851950 MtrunA17_Chr7g0218081 46.552 58 28 1 206 263 410 464 4.83e-11 63.9
Msa0851950 MtrunA17_Chr4g0051881 56.897 58 22 1 206 263 178 232 9.08e-15 74.7
Msa0851950 MtrunA17_Chr8g0383351 50.847 59 26 1 206 264 160 215 9.80e-15 72.8
Msa0851950 MtrunA17_Chr2g0319961 41.509 106 50 4 175 275 47 145 1.06e-14 73.2
Msa0851950 MtrunA17_Chr5g0428351 45.588 68 37 0 207 274 184 251 1.06e-14 73.9
Msa0851950 MtrunA17_Chr8g0386241 50.847 59 26 1 206 264 103 158 1.41e-14 71.2
Msa0851950 MtrunA17_Chr5g0418191 47.059 68 32 2 196 263 141 204 1.68e-14 74.3
Msa0851950 MtrunA17_Chr5g0418191 48.276 58 27 1 206 263 320 374 1.03e-11 65.9
Msa0851950 MtrunA17_Chr2g0315371 50.794 63 31 0 206 268 141 203 2.80e-14 72.8
Msa0851950 MtrunA17_Chr7g0228941 52.542 59 26 1 205 263 51 107 3.19e-14 68.9
Msa0851950 MtrunA17_Chr4g0053281 44.706 85 43 3 179 263 241 321 3.39e-14 72.8
Msa0851950 MtrunA17_Chr7g0248721 54.688 64 23 3 206 267 141 200 4.76e-14 70.5
Msa0851950 MtrunA17_Chr1g0184691 43.902 82 41 3 195 274 281 359 5.98e-14 72.4
Msa0851950 MtrunA17_Chr7g0238921 52.542 59 25 1 206 264 124 179 7.32e-14 71.6
Msa0851950 MtrunA17_Chr3g0139021 52.542 59 26 1 205 263 313 369 7.70e-14 72.4
Msa0851950 MtrunA17_Chr7g0249311 51.724 58 24 2 206 263 179 232 1.15e-13 72.4
Msa0851950 MtrunA17_Chr3g0129361 51.724 58 25 1 206 263 144 198 1.18e-13 69.7
Msa0851950 MtrunA17_Chr7g0270191 40.404 99 49 3 206 297 145 240 2.04e-13 70.1
Msa0851950 MtrunA17_Chr5g0401821 41.176 85 46 3 180 263 173 254 3.28e-13 69.3
Msa0851950 MtrunA17_Chr1g0194481 51.724 58 25 1 206 263 158 212 4.88e-13 69.3
Msa0851950 MtrunA17_Chr8g0335811 51.724 58 25 1 206 263 154 208 5.72e-13 67.8
Msa0851950 MtrunA17_Chr4g0042831 35.106 94 50 2 200 293 139 221 7.07e-13 69.3
Msa0851950 MtrunA17_Chr4g0042831 50.000 58 26 1 206 263 278 332 3.18e-12 67.4
Msa0851950 MtrunA17_Chr2g0294231 44.737 76 33 2 206 281 203 269 1.61e-12 68.2
Msa0851950 MtrunA17_Chr5g0445311 48.276 58 27 1 206 263 364 418 1.73e-12 68.6
Msa0851950 MtrunA17_Chr5g0445311 47.368 57 26 2 207 263 201 253 4.12e-11 64.3
Msa0851950 MtrunA17_Chr2g0322391 53.448 58 25 1 207 264 350 405 2.27e-12 68.2
Msa0851950 MtrunA17_Chr3g0101131 48.276 58 27 1 206 263 415 469 2.34e-12 68.2
Msa0851950 MtrunA17_Chr8g0387501 54.386 57 24 1 207 263 130 184 2.40e-12 66.6
Msa0851950 MtrunA17_Chr3g0106491 36.697 109 53 4 196 303 188 281 3.38e-12 67.4
Msa0851950 MtrunA17_Chr3g0099641 50.877 57 26 1 207 263 252 306 4.09e-12 67.0
Msa0851950 MtrunA17_Chr8g0348311 54.386 57 24 1 207 263 156 210 4.24e-12 66.6
Msa0851950 MtrunA17_Chr4g0075201 46.575 73 27 2 207 279 268 328 4.32e-12 67.0
Msa0851950 MtrunA17_Chr2g0288871 52.632 57 25 1 207 263 306 360 5.38e-12 67.0
Msa0851950 MtrunA17_Chr2g0289181 47.458 59 29 1 205 263 13 69 5.41e-12 65.1
Msa0851950 MtrunA17_Chr5g0432161 48.276 58 26 2 206 263 244 297 5.42e-12 67.0
Msa0851950 MtrunA17_Chr5g0432161 48.276 58 27 1 206 263 421 475 1.59e-11 65.5
Msa0851950 MtrunA17_Chr7g0270831 52.632 57 25 1 207 263 217 271 5.90e-12 66.6
Msa0851950 MtrunA17_Chr1g0150281 49.123 57 27 1 207 263 141 195 8.83e-12 65.1
Msa0851950 MtrunA17_Chr7g0248691 42.529 87 41 3 177 263 132 209 1.02e-11 65.5
Msa0851950 MtrunA17_Chr1g0202461 50.877 57 26 1 207 263 262 316 1.23e-11 65.5
Msa0851950 MtrunA17_Chr7g0243891 44.928 69 34 2 195 263 205 269 1.42e-11 65.9
Msa0851950 MtrunA17_Chr4g0004911 44.828 58 29 1 206 263 184 238 1.52e-11 65.5
Msa0851950 MtrunA17_Chr6g0485431 51.724 58 24 2 207 263 155 209 1.56e-11 65.1
Msa0851950 MtrunA17_Chr7g0270051 49.153 59 28 1 205 263 52 108 1.76e-11 63.9
Msa0851950 MtrunA17_Chr3g0136961 44.615 65 34 1 199 263 131 193 2.22e-11 63.9
Msa0851950 MtrunA17_Chr4g0068541 45.763 59 29 1 206 264 208 263 2.44e-11 64.7
Msa0851950 MtrunA17_Chr7g0249301 46.552 58 27 2 206 263 62 115 5.15e-11 63.5
Msa0851950 MtrunA17_Chr4g0060271 52.632 57 25 1 207 263 256 310 6.15e-11 63.9
Msa0851950 MtrunA17_Chr5g0447701 47.368 57 27 1 207 263 253 306 9.70e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0851950 AT2G40740.1 34.659 352 149 10 1 347 11 286 2.51e-53 178
Msa0851950 AT2G40740.3 34.659 352 149 10 1 347 20 295 5.67e-53 177
Msa0851950 AT4G11070.2 52.174 92 42 2 182 272 85 175 8.26e-23 97.1
Msa0851950 AT4G11070.1 52.174 92 42 2 182 272 117 207 1.26e-22 97.1
Msa0851950 AT4G23810.1 59.420 69 28 0 202 270 154 222 2.16e-22 96.7
Msa0851950 AT5G24110.1 45.918 98 52 1 179 276 87 183 6.91e-22 94.7
Msa0851950 AT2G40740.2 42.188 128 42 5 222 347 145 242 1.15e-20 90.5
Msa0851950 AT3G56400.1 52.174 69 33 0 206 274 120 188 7.42e-20 89.0
Msa0851950 AT2G46400.1 36.429 140 80 4 150 289 56 186 1.78e-19 87.8
Msa0851950 AT1G66560.1 45.055 91 43 1 175 265 79 162 2.07e-18 84.0
Msa0851950 AT1G66550.2 52.381 63 30 0 203 265 100 162 2.02e-17 81.3
Msa0851950 AT1G66550.1 52.381 63 30 0 203 265 105 167 2.46e-17 81.3
Msa0851950 AT5G01900.1 55.932 59 26 0 207 265 111 169 6.66e-17 80.1
Msa0851950 AT2G40750.1 53.333 60 28 0 206 265 152 211 7.33e-17 81.3
Msa0851950 AT2G30590.1 33.333 144 88 5 124 263 229 368 1.42e-16 80.5
Msa0851950 AT1G29280.1 45.161 93 40 4 177 263 42 129 2.47e-16 78.6
Msa0851950 AT2G46130.1 56.897 58 22 1 206 263 30 84 3.16e-16 74.3
Msa0851950 AT1G64000.1 55.172 58 23 1 206 263 114 168 5.37e-16 76.3
Msa0851950 AT1G69310.4 49.254 67 30 2 206 272 147 209 6.00e-16 77.8
Msa0851950 AT1G69310.2 49.254 67 30 2 206 272 147 209 6.00e-16 77.8
Msa0851950 AT1G69310.1 49.254 67 30 2 206 272 147 209 6.00e-16 77.8
Msa0851950 AT4G26440.1 49.351 77 26 3 204 280 176 239 8.52e-16 79.0
Msa0851950 AT5G45260.1 31.343 134 88 1 134 263 1132 1265 1.85e-15 78.6
Msa0851950 AT5G41570.1 55.172 58 23 1 206 263 98 152 2.11e-15 73.9
Msa0851950 AT4G18170.1 28.302 265 157 9 77 339 59 292 2.39e-15 76.3
Msa0851950 AT4G31550.2 42.857 84 45 2 180 263 220 300 2.43e-15 76.6
Msa0851950 AT4G31550.1 47.059 85 41 3 179 263 221 301 2.94e-15 76.3
Msa0851950 AT2G34830.2 44.186 86 42 2 182 263 196 279 3.21e-15 77.0
Msa0851950 AT2G34830.1 44.186 86 42 2 182 263 187 270 3.60e-15 76.6
Msa0851950 AT1G66600.1 36.975 119 54 3 147 265 65 162 4.37e-15 74.3
Msa0851950 AT2G24570.1 45.556 90 45 3 179 268 218 303 5.86e-15 75.5
Msa0851950 AT2G37260.1 44.860 107 52 3 174 280 321 420 7.28e-15 75.9
Msa0851950 AT2G37260.1 54.386 57 22 2 207 263 166 218 1.56e-13 72.0
Msa0851950 AT2G37260.3 44.860 107 52 3 174 280 241 340 8.68e-15 75.1
Msa0851950 AT2G37260.3 54.386 57 22 2 207 263 86 138 1.75e-13 71.2
Msa0851950 AT2G37260.2 44.860 107 52 3 174 280 239 338 8.88e-15 75.1
Msa0851950 AT2G37260.2 54.386 57 22 2 207 263 84 136 1.74e-13 71.2
Msa0851950 AT3G58710.1 44.565 92 42 4 177 263 39 126 9.68e-15 73.9
Msa0851950 AT1G29860.1 56.897 58 22 1 206 263 136 190 1.11e-14 73.9
Msa0851950 AT2G23320.1 40.000 85 47 3 180 263 214 295 1.11e-14 74.3
Msa0851950 AT3G01080.3 50.000 62 27 2 202 263 201 258 1.95e-14 73.9
Msa0851950 AT1G30650.1 43.023 86 43 2 182 263 189 272 1.97e-14 74.7
Msa0851950 AT2G03340.1 48.529 68 29 3 197 263 241 303 2.00e-14 74.7
Msa0851950 AT2G03340.1 42.169 83 45 1 206 288 415 494 5.24e-12 67.4
Msa0851950 AT3G58710.2 41.489 94 47 3 175 263 35 125 2.39e-14 72.8
Msa0851950 AT4G39410.1 41.667 84 39 2 206 282 223 303 5.13e-14 72.4
Msa0851950 AT3G01080.2 50.000 62 27 2 202 263 201 258 5.64e-14 73.2
Msa0851950 AT5G28650.1 44.706 85 43 3 180 263 236 317 5.72e-14 72.4
Msa0851950 AT5G46350.1 53.448 58 24 1 206 263 183 237 8.36e-14 72.0
Msa0851950 AT3G01080.1 50.000 62 27 2 202 263 163 220 8.52e-14 72.8
Msa0851950 AT1G13960.1 45.833 72 33 3 193 263 216 282 1.18e-13 72.4
Msa0851950 AT1G13960.1 48.276 58 27 1 206 263 409 463 2.17e-11 65.5
Msa0851950 AT5G13080.1 53.448 58 24 1 206 263 67 121 1.21e-13 68.2
Msa0851950 AT5G22570.1 47.458 59 31 0 207 265 111 169 1.45e-13 70.9
Msa0851950 AT3G04670.1 40.206 97 54 3 180 275 236 329 1.77e-13 70.9
Msa0851950 AT1G13960.2 45.833 72 33 3 193 263 63 129 2.00e-13 71.2
Msa0851950 AT1G13960.2 48.276 58 27 1 206 263 256 310 4.77e-11 63.9
Msa0851950 AT5G52830.1 50.000 82 33 3 182 263 147 220 2.46e-13 70.9
Msa0851950 AT2G44745.1 51.724 58 25 1 206 263 145 199 2.61e-13 68.9
Msa0851950 AT5G56270.1 46.753 77 28 3 204 280 271 334 4.33e-13 70.9
Msa0851950 AT5G49520.1 51.724 58 25 1 206 263 221 275 4.48e-13 70.5
Msa0851950 AT1G80590.1 49.153 59 30 0 207 265 86 144 4.99e-13 68.6
Msa0851950 AT4G23550.1 54.386 57 24 1 207 263 141 195 5.89e-13 69.3
Msa0851950 AT2G38470.1 43.182 88 40 4 182 263 154 237 9.80e-13 69.7
Msa0851950 AT4G04450.2 42.045 88 38 3 207 294 199 273 1.53e-12 68.9
Msa0851950 AT2G47260.1 38.462 91 53 1 181 271 149 236 2.02e-12 67.8
Msa0851950 AT4G04450.1 42.045 88 38 3 207 294 293 367 2.06e-12 68.6
Msa0851950 AT4G01250.1 39.759 83 43 2 181 263 108 183 2.20e-12 67.4
Msa0851950 AT4G31800.2 43.284 67 36 1 197 263 102 166 2.40e-12 66.6
Msa0851950 AT4G31800.1 43.284 67 36 1 197 263 167 231 3.48e-12 67.0
Msa0851950 AT4G26640.7 48.276 58 27 1 206 263 341 395 4.09e-12 67.8
Msa0851950 AT4G26640.2 48.276 58 27 1 206 263 381 435 4.24e-12 67.8
Msa0851950 AT4G24240.1 40.659 91 50 3 174 263 249 336 4.52e-12 67.0
Msa0851950 AT4G26640.5 48.276 58 27 1 206 263 205 259 4.64e-12 67.0
Msa0851950 AT4G26640.6 48.276 58 27 1 206 263 205 259 4.64e-12 67.0
Msa0851950 AT4G26640.4 48.276 58 27 1 206 263 205 259 4.64e-12 67.0
Msa0851950 AT4G26640.3 48.276 58 27 1 206 263 205 259 4.64e-12 67.0
Msa0851950 AT4G26640.1 48.276 58 27 1 206 263 309 363 4.66e-12 67.4
Msa0851950 AT2G04880.2 49.123 57 26 1 207 263 284 337 8.86e-12 66.6
Msa0851950 AT2G04880.2 51.724 58 24 2 206 263 111 164 2.24e-11 65.5
Msa0851950 AT5G26170.1 44.828 58 29 1 206 263 113 167 8.91e-12 63.5
Msa0851950 AT2G04880.1 49.123 57 26 1 207 263 308 361 9.95e-12 66.6
Msa0851950 AT2G04880.1 51.724 58 24 2 206 263 111 164 2.18e-11 65.5
Msa0851950 AT5G45050.1 43.529 85 41 2 179 263 1158 1235 1.04e-11 67.0
Msa0851950 AT5G45050.2 43.529 85 41 2 179 263 1130 1207 1.07e-11 67.0
Msa0851950 AT5G45050.4 43.529 85 41 2 179 263 1019 1096 1.12e-11 67.0
Msa0851950 AT1G62300.1 52.632 57 25 1 207 263 313 367 1.38e-11 66.2
Msa0851950 AT4G01720.1 52.632 57 25 1 207 263 240 294 1.62e-11 65.9
Msa0851950 AT4G30935.1 41.558 77 41 2 207 283 332 404 2.02e-11 65.5
Msa0851950 AT4G22070.4 52.632 57 25 1 207 263 298 352 3.64e-11 64.7
Msa0851950 AT4G22070.3 52.632 57 25 1 207 263 298 352 3.64e-11 64.7
Msa0851950 AT4G22070.2 52.632 57 25 1 207 263 298 352 3.64e-11 64.7
Msa0851950 AT4G22070.1 52.632 57 25 1 207 263 298 352 3.64e-11 64.7
Msa0851950 AT3G01970.1 48.276 58 27 1 206 263 65 119 4.26e-11 60.8
Msa0851950 AT1G69310.3 43.284 67 30 3 206 272 147 205 8.05e-11 62.8
Msa0851950 AT2G30250.1 52.632 57 24 1 207 263 329 382 8.74e-11 63.2
Msa0851950 AT2G25000.2 37.500 80 48 1 184 263 81 158 9.65e-11 61.6
Msa0851950 AT2G25000.4 37.500 80 48 1 184 263 81 158 9.65e-11 61.6
Msa0851950 AT5G43290.1 49.153 59 27 1 206 264 114 169 9.66e-11 62.4

Find 96 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5_4:-42621292 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5_4:-42624312 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5_4:-42624344 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.259697 5_4:-42621344 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5_4:+42621676 Msa0851950:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5_4:+42623391 Msa0851950:CDS
TGAAATGGGTGGGAGGGATA+TGG 0.320333 5_4:+42621645 Msa0851950:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.320492 5_4:+42621630 Msa0851950:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5_4:+42624553 Msa0851950:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5_4:+42621457 Msa0851950:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5_4:-42624472 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5_4:-42624308 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5_4:-42621275 None:intergenic
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5_4:+42624598 Msa0851950:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5_4:-42623382 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.382607 5_4:+42621605 Msa0851950:CDS
ATTAGAACACTCACATCAAA+AGG 0.383819 5_4:-42621544 None:intergenic
CATTTATCAGAAGTTACTAT+AGG 0.384161 5_4:+42624258 Msa0851950:intron
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5_4:-42621409 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5_4:+42621667 Msa0851950:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5_4:+42621498 Msa0851950:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5_4:+42623306 Msa0851950:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5_4:+42623380 Msa0851950:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5_4:-42621274 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5_4:+42624541 Msa0851950:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5_4:+42624486 Msa0851950:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5_4:-42623349 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5_4:-42624450 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5_4:-42624343 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5_4:+42621466 Msa0851950:CDS
CGTTCAGTTGTTAAGGTCTA+GGG 0.444913 5_4:+42621606 Msa0851950:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5_4:-42624471 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5_4:-42624425 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.450200 5_4:+42621631 Msa0851950:CDS
GAACATTACATCGGCCATAT+CGG 0.452060 5_4:-42624567 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5_4:-42624416 None:intergenic
ATATGATGATGGTGCTGTTA+AGG 0.457432 5_4:-42624393 None:intergenic
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5_4:-42624462 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5_4:-42621206 None:intergenic
GGTGGTGGTGGATATGATGA+TGG 0.483740 5_4:-42624404 None:intergenic
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5_4:-42624485 None:intergenic
GGACCCTCTACTTCAAGATT+CGG 0.492644 5_4:+42624527 Msa0851950:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5_4:-42624496 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5_4:+42624503 Msa0851950:CDS
TCTAGGGGAACTTTGAGGAT+TGG 0.506422 5_4:+42621622 Msa0851950:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5_4:+42623343 Msa0851950:CDS
TAAGGTCTAGGGGAACTTTG+AGG 0.511706 5_4:+42621617 Msa0851950:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5_4:+42621599 Msa0851950:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5_4:+42624497 Msa0851950:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5_4:+42624469 Msa0851950:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5_4:-42621291 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5_4:+42624350 Msa0851950:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5_4:-42623355 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5_4:+42621460 Msa0851950:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5_4:+42623370 Msa0851950:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5_4:-42621284 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5_4:+42621577 Msa0851950:CDS
AAGGTTTAGATAGATCAAAA+GGG 0.540645 5_4:+42621668 Msa0851950:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5_4:+42621565 Msa0851950:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5_4:+42624455 Msa0851950:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5_4:-42624430 None:intergenic
TGCGGTGAACCACGACCTCA+GGG 0.552440 5_4:+42624487 Msa0851950:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5_4:-42624482 None:intergenic
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5_4:-42624422 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5_4:+42621634 Msa0851950:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5_4:+42624506 Msa0851950:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5_4:+42621635 Msa0851950:CDS
TAATTTACCTTGGGTACTTG+CGG 0.567600 5_4:-42623432 None:intergenic
GGCCGATGTAATGTTCAACT+CGG 0.582893 5_4:+42624574 Msa0851950:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5_4:+42624456 Msa0851950:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5_4:+42621638 Msa0851950:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5_4:-42624530 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5_4:-42621543 None:intergenic
TACTTGGCCGCAAGTACCCA+AGG 0.596156 5_4:+42623425 Msa0851950:CDS
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5_4:+42624459 Msa0851950:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5_4:+42624587 Msa0851950:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.607995 5_4:+42621639 Msa0851950:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5_4:-42621419 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5_4:+42624643 Msa0851950:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5_4:-42624419 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5_4:+42624575 Msa0851950:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5_4:-42624531 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5_4:-42623379 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5_4:+42621568 Msa0851950:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5_4:+42621571 Msa0851950:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5_4:-42624560 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5_4:+42624491 Msa0851950:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5_4:+42621580 Msa0851950:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5_4:+42621261 Msa0851950:CDS
GTTCAGTTGTTAAGGTCTAG+GGG 0.665415 5_4:+42621607 Msa0851950:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5_4:+42624544 Msa0851950:CDS
ATGGGTGGGAGGGATATGGA+AGG 0.678067 5_4:+42621649 Msa0851950:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5_4:-42624502 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5_4:-42624576 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5_4:+42624488 Msa0851950:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5_4:-42621288 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAATCAATTTTTTTTTTA+TGG - chr5_4:42622830-42622849 None:intergenic 10.0%
!!! AAAATTGATTTGAATTTTTT+TGG + chr5_4:42622837-42622856 Msa0851950:intron 10.0%
!!! TTTTACATAAAATTATAAGT+AGG + chr5_4:42623207-42623226 Msa0851950:intron 10.0%
!! TAATTGTACTTTATAAATAT+AGG + chr5_4:42623467-42623486 Msa0851950:intron 10.0%
!! AATTGTACTTTATAAATATA+GGG + chr5_4:42623468-42623487 Msa0851950:intron 10.0%
!!! TTATATACATACCATTTTTT+TGG - chr5_4:42621739-42621758 None:intergenic 15.0%
!! TCAAAGTTTCATATATTAAA+TGG + chr5_4:42621821-42621840 Msa0851950:intron 15.0%
!!! TCACTAGTTAATTATTTTAA+TGG + chr5_4:42623016-42623035 Msa0851950:intron 15.0%
!!! AGTAAAGTGAATTTTTTTTT+TGG - chr5_4:42623120-42623139 None:intergenic 15.0%
!!! TTTCCATAATTTTTTTTTGA+AGG + chr5_4:42623895-42623914 Msa0851950:intron 15.0%
!! TTTCCTTCAAAAAAAAATTA+TGG - chr5_4:42623901-42623920 None:intergenic 15.0%
!!! AGTTTCTAAATCATTTTTTA+AGG + chr5_4:42624020-42624039 Msa0851950:intron 15.0%
!!! CGCTTTATTATATAATAATT+TGG + chr5_4:42624071-42624090 Msa0851950:intron 15.0%
!!! AAAAAAAAAAAATTTGTTGG+TGG - chr5_4:42624179-42624198 None:intergenic 15.0%
!! CCAAAAAAAAAAAAATTTGT+TGG - chr5_4:42624182-42624201 None:intergenic 15.0%
!!! CCAACAAATTTTTTTTTTTT+TGG + chr5_4:42624179-42624198 Msa0851950:intron 15.0%
!!! ATTTCTAAGGTATTGAATTT+AGG + chr5_4:42621930-42621949 Msa0851950:intron 20.0%
!! AAAAATCTTATGGAAATGAT+TGG - chr5_4:42622331-42622350 None:intergenic 20.0%
!! TTTCTTTCAATTATTGTTGT+TGG - chr5_4:42622551-42622570 None:intergenic 20.0%
!! AAATTTATATATGCAAGATC+CGG + chr5_4:42623067-42623086 Msa0851950:intron 20.0%
!! AATAGTTTGACACAATTATT+AGG + chr5_4:42623144-42623163 Msa0851950:intron 20.0%
!! CAATTATTAGGAAGTTAATA+TGG + chr5_4:42623156-42623175 Msa0851950:intron 20.0%
!! AATTAGTGTTAATTTACCTT+GGG - chr5_4:42623444-42623463 None:intergenic 20.0%
!! TAATTAGTGTTAATTTACCT+TGG - chr5_4:42623445-42623464 None:intergenic 20.0%
!! AAGAAATATTCTTAGAAGAA+AGG - chr5_4:42623575-42623594 None:intergenic 20.0%
!!! TTTCTTCTAAGAATATTTCT+TGG + chr5_4:42623574-42623593 Msa0851950:intron 20.0%
!! AAGTATTTAACAAGTAATCA+AGG - chr5_4:42623779-42623798 None:intergenic 20.0%
!!! TTTTATCTAACTGAAGATAA+AGG + chr5_4:42624629-42624648 Msa0851950:CDS 20.0%
! ATTGAGAATAAGATGATAAG+TGG + chr5_4:42621565-42621584 Msa0851950:CDS 25.0%
! AAGGTTTAGATAGATCAAAA+GGG + chr5_4:42621668-42621687 Msa0851950:CDS 25.0%
! ATTCAATAACATAGATTGTG+AGG - chr5_4:42621859-42621878 None:intergenic 25.0%
! ATGTTATTGAATCAAAGTCT+TGG + chr5_4:42621867-42621886 Msa0851950:intron 25.0%
!!! CAACACAAAGATTATTTCTA+AGG + chr5_4:42621917-42621936 Msa0851950:intron 25.0%
!!! CATATTTGGGTTTTAGTTTA+TGG + chr5_4:42622010-42622029 Msa0851950:intron 25.0%
! TTGCAATTCAACTGAAATTA+AGG - chr5_4:42622100-42622119 None:intergenic 25.0%
! ATACATCAAAGTTGAAAGTT+AGG - chr5_4:42622172-42622191 None:intergenic 25.0%
!! TAGGGTGAAAATTTTCATTA+TGG - chr5_4:42622268-42622287 None:intergenic 25.0%
! TAATGTGAAAATCCTAAGTA+GGG - chr5_4:42622286-42622305 None:intergenic 25.0%
! ATAATGTGAAAATCCTAAGT+AGG - chr5_4:42622287-42622306 None:intergenic 25.0%
!! TTCCATAAGATTTTTACTAG+AGG + chr5_4:42622336-42622355 Msa0851950:intron 25.0%
!! TCCATAAGATTTTTACTAGA+GGG + chr5_4:42622337-42622356 Msa0851950:intron 25.0%
! TAAAAGTAGAGATTGAAATG+AGG + chr5_4:42622393-42622412 Msa0851950:intron 25.0%
!! ATGTAAGATAGAATTTTCCA+TGG + chr5_4:42622671-42622690 Msa0851950:intron 25.0%
!!! TTTTTTGGTGTTTGCTATTT+TGG + chr5_4:42622852-42622871 Msa0851950:intron 25.0%
! AATTTATATATGCAAGATCC+GGG + chr5_4:42623068-42623087 Msa0851950:intron 25.0%
!!! ATTTTTTTTTTGGTGATGAC+CGG - chr5_4:42623110-42623129 None:intergenic 25.0%
! GTATAATTCAGTTGTTAGAT+TGG + chr5_4:42623178-42623197 Msa0851950:intron 25.0%
!! ATTCTTAGAAGAAAGGTATA+AGG - chr5_4:42623568-42623587 None:intergenic 25.0%
! ATTTCAAATTAGCCAACAAA+GGG - chr5_4:42624230-42624249 None:intergenic 25.0%
! TATTTCAAATTAGCCAACAA+AGG - chr5_4:42624231-42624250 None:intergenic 25.0%
! CATTTATCAGAAGTTACTAT+AGG + chr5_4:42624258-42624277 Msa0851950:intron 25.0%
!! CTGGACACAAATATAATTTT+TGG - chr5_4:42624292-42624311 None:intergenic 25.0%
!! ATGTTACTTCAACTATGTTA+GGG - chr5_4:42624346-42624365 None:intergenic 25.0%
!! TATGTTACTTCAACTATGTT+AGG - chr5_4:42624347-42624366 None:intergenic 25.0%
! ATAGTTGAAGTAACATATAG+AGG + chr5_4:42624350-42624369 Msa0851950:CDS 25.0%
!!! TGCTTTTTCTACATCATCAA+TGG - chr5_4:42621314-42621333 None:intergenic 30.0%
CATCATCATTACTCTTTCTT+TGG - chr5_4:42621347-42621366 None:intergenic 30.0%
ATTTGAGCAAAGGAATTAGA+AGG - chr5_4:42621412-42621431 None:intergenic 30.0%
AAAACTCAACATGCTCAAAT+TGG + chr5_4:42621457-42621476 Msa0851950:CDS 30.0%
TTAGAACACTCACATCAAAA+GGG - chr5_4:42621546-42621565 None:intergenic 30.0%
ATTAGAACACTCACATCAAA+AGG - chr5_4:42621547-42621566 None:intergenic 30.0%
GAAGGTTTAGATAGATCAAA+AGG + chr5_4:42621667-42621686 Msa0851950:CDS 30.0%
!!! TACCATTTTTTTGGTCTTGA+AGG - chr5_4:42621730-42621749 None:intergenic 30.0%
ACCGGGTAATTTACATATTT+GGG + chr5_4:42621997-42622016 Msa0851950:intron 30.0%
ACCCAAATATGTAAATTACC+CGG - chr5_4:42622001-42622020 None:intergenic 30.0%
!!! GGGTTTTAGTTTATGGTTAT+AGG + chr5_4:42622017-42622036 Msa0851950:intron 30.0%
! TTCTGTAAGATTTTCACTAG+AGG + chr5_4:42622238-42622257 Msa0851950:intron 30.0%
! TCTGTAAGATTTTCACTAGA+GGG + chr5_4:42622239-42622258 Msa0851950:intron 30.0%
! TGAAAATTTTCACCCTACTT+AGG + chr5_4:42622271-42622290 Msa0851950:intron 30.0%
TCCCTCTAGTAAAAATCTTA+TGG - chr5_4:42622341-42622360 None:intergenic 30.0%
GATTACTCAATGTTTGTACT+AGG - chr5_4:42622373-42622392 None:intergenic 30.0%
AGATTGAAATGAGGTTAACT+TGG + chr5_4:42622402-42622421 Msa0851950:intron 30.0%
! AGGTCACTAGTTTATGAAAA+TGG + chr5_4:42622468-42622487 Msa0851950:intron 30.0%
TCTACAATTGTGACAAAAAC+AGG - chr5_4:42622510-42622529 None:intergenic 30.0%
! CTTTCAATTATTGTTGTTGG+CGG - chr5_4:42622548-42622567 None:intergenic 30.0%
! ACAAAAGTGAAATGCTTTTG+AGG + chr5_4:42622575-42622594 Msa0851950:intron 30.0%
! GGTATTGAAAGCTTTAGTTT+AGG + chr5_4:42622596-42622615 Msa0851950:intron 30.0%
AACACACCAATAATAACCTA+CGG - chr5_4:42622624-42622643 None:intergenic 30.0%
CTAATTACTAAGAAAGACCA+TGG - chr5_4:42622691-42622710 None:intergenic 30.0%
CATGGTCTTTCTTAGTAATT+AGG + chr5_4:42622689-42622708 Msa0851950:intron 30.0%
TAGTTTACATGACCAAAACA+AGG + chr5_4:42622762-42622781 Msa0851950:intron 30.0%
! AGGTCTTTGCTATAGTATTT+AGG + chr5_4:42622782-42622801 Msa0851950:intron 30.0%
ATTTATATATGCAAGATCCG+GGG + chr5_4:42623069-42623088 Msa0851950:intron 30.0%
!!! TTTTTTTTTTGGTGATGACC+GGG - chr5_4:42623109-42623128 None:intergenic 30.0%
TATGGCCAAAAAGAAATACT+TGG + chr5_4:42623409-42623428 Msa0851950:CDS 30.0%
!! AAGAAAGGTATAAGGTTTTC+TGG - chr5_4:42623560-42623579 None:intergenic 30.0%
TGTGAATGAGTCAATTCATA+GGG - chr5_4:42623633-42623652 None:intergenic 30.0%
GTACTAGTTGTGATGAAAAA+GGG - chr5_4:42623709-42623728 None:intergenic 30.0%
AGTACTAGTTGTGATGAAAA+AGG - chr5_4:42623710-42623729 None:intergenic 30.0%
AACAATGCATAATATACGCA+AGG + chr5_4:42624134-42624153 Msa0851950:intron 30.0%
!! AAAAAAATTTGTTGGTGGCT+GGG - chr5_4:42624174-42624193 None:intergenic 30.0%
!! AAAAAAAATTTGTTGGTGGC+TGG - chr5_4:42624175-42624194 None:intergenic 30.0%
ATTCATATTATCCCCTTTGT+TGG + chr5_4:42624215-42624234 Msa0851950:intron 30.0%
TTTCAAATTAGCCAACAAAG+GGG - chr5_4:42624229-42624248 None:intergenic 30.0%
TGGATGAGAATAGCATTTCA+GGG - chr5_4:42621294-42621313 None:intergenic 35.0%
ATGGATGAGAATAGCATTTC+AGG - chr5_4:42621295-42621314 None:intergenic 35.0%
ATGAGACAGAATTTGAGCAA+AGG - chr5_4:42621422-42621441 None:intergenic 35.0%
TTCAATGCATGCACATTCAA+TGG + chr5_4:42621498-42621517 Msa0851950:CDS 35.0%
GAGAATAAGATGATAAGTGG+CGG + chr5_4:42621568-42621587 Msa0851950:CDS 35.0%
! AACTTTGAGGATTGGTGAAA+TGG + chr5_4:42621630-42621649 Msa0851950:CDS 35.0%
! ACTTTGAGGATTGGTGAAAT+GGG + chr5_4:42621631-42621650 Msa0851950:CDS 35.0%
GATAGATCAAAAGGGTTAGA+AGG + chr5_4:42621676-42621695 Msa0851950:CDS 35.0%
!!! CATTTTTTTGGTCTTGAAGG+TGG - chr5_4:42621727-42621746 None:intergenic 35.0%
CACCTTCAAGACCAAAAAAA+TGG + chr5_4:42621725-42621744 Msa0851950:CDS 35.0%
! GGTATTGAATTTAGGTGTTG+AGG + chr5_4:42621938-42621957 Msa0851950:intron 35.0%
! TTTCAGTGTTTTTCCAACAC+CGG + chr5_4:42621979-42621998 Msa0851950:intron 35.0%
ATATGTAAATTACCCGGTGT+TGG - chr5_4:42621995-42622014 None:intergenic 35.0%
CACCGGGTAATTTACATATT+TGG + chr5_4:42621996-42622015 Msa0851950:intron 35.0%
TTATAGGTGCCTTGCTAATT+GGG + chr5_4:42622033-42622052 Msa0851950:intron 35.0%
TTGCGTTAAGATGTGACATA+TGG + chr5_4:42622072-42622091 Msa0851950:intron 35.0%
TGCGTTAAGATGTGACATAT+GGG + chr5_4:42622073-42622092 Msa0851950:intron 35.0%
ATGTGTGTGTGATTGAATCT+AGG + chr5_4:42622432-42622451 Msa0851950:intron 35.0%
AACTAGTGACCTAATTAGCA+AGG - chr5_4:42622460-42622479 None:intergenic 35.0%
TAGGATCCGTAGGTTATTAT+TGG + chr5_4:42622615-42622634 Msa0851950:intron 35.0%
TGGTGTGTTTGTAAACAGAA+AGG + chr5_4:42622635-42622654 Msa0851950:intron 35.0%
AATTAGGCAACAAGTCATCA+TGG + chr5_4:42622705-42622724 Msa0851950:intron 35.0%
!! CTATAGCAAAGACCTTGTTT+TGG - chr5_4:42622777-42622796 None:intergenic 35.0%
GTGTCTAAGAACTGTGTATT+TGG + chr5_4:42622878-42622897 Msa0851950:intron 35.0%
!!! TTTTTTTTTGGTGATGACCG+GGG - chr5_4:42623108-42623127 None:intergenic 35.0%
TCACACACAAACACACATAT+AGG - chr5_4:42623248-42623267 None:intergenic 35.0%
! ATGTGAAGGCACAACAAATT+AGG - chr5_4:42623279-42623298 None:intergenic 35.0%
GGCTTTACTTGGAGAAAATA+TGG + chr5_4:42623391-42623410 Msa0851950:CDS 35.0%
TAATTTACCTTGGGTACTTG+CGG - chr5_4:42623435-42623454 None:intergenic 35.0%
! AAGGTATAAGGTTTTCTGGA+TGG - chr5_4:42623556-42623575 None:intergenic 35.0%
GTGTGAATGAGTCAATTCAT+AGG - chr5_4:42623634-42623653 None:intergenic 35.0%
CATAATATACGCAAGGTTTG+AGG + chr5_4:42624141-42624160 Msa0851950:intron 35.0%
!! AAAAAATTTGTTGGTGGCTG+GGG - chr5_4:42624173-42624192 None:intergenic 35.0%
!! ATATGATGATGGTGCTGTTA+AGG - chr5_4:42624396-42624415 None:intergenic 35.0%
CTAATGTTCACAAGTAGGTT+TGG - chr5_4:42624428-42624447 None:intergenic 35.0%
TGGTACTAATGTTCACAAGT+AGG - chr5_4:42624433-42624452 None:intergenic 35.0%
!! AAGATTCGGCATTGATTATC+CGG + chr5_4:42624541-42624560 Msa0851950:CDS 35.0%
TGAACTAGAACTACCCAACA+TGG + chr5_4:42621261-42621280 Msa0851950:CDS 40.0%
ATGAGAATAGCATTTCAGGG+TGG - chr5_4:42621291-42621310 None:intergenic 40.0%
ACTCAACATGCTCAAATTGG+TGG + chr5_4:42621460-42621479 Msa0851950:CDS 40.0%
AATAAGATGATAAGTGGCGG+TGG + chr5_4:42621571-42621590 Msa0851950:CDS 40.0%
ACGTTCAGTTGTTAAGGTCT+AGG + chr5_4:42621605-42621624 Msa0851950:CDS 40.0%
CGTTCAGTTGTTAAGGTCTA+GGG + chr5_4:42621606-42621625 Msa0851950:CDS 40.0%
GTTCAGTTGTTAAGGTCTAG+GGG + chr5_4:42621607-42621626 Msa0851950:CDS 40.0%
! TTCAGTGTTTTTCCAACACC+GGG + chr5_4:42621980-42621999 Msa0851950:intron 40.0%
GTTATAGGTGCCTTGCTAAT+TGG + chr5_4:42622032-42622051 Msa0851950:intron 40.0%
ATCTAGTTGCCCAATTAGCA+AGG - chr5_4:42622045-42622064 None:intergenic 40.0%
CAAAGTTGAAAGTTAGGTCG+TGG - chr5_4:42622166-42622185 None:intergenic 40.0%
TCAATCACACACACATTGTG+AGG - chr5_4:42622428-42622447 None:intergenic 40.0%
ATCTAGGTGCCTTGCTAATT+AGG + chr5_4:42622448-42622467 Msa0851950:intron 40.0%
! AGCTTTAGTTTAGGATCCGT+AGG + chr5_4:42622605-42622624 Msa0851950:intron 40.0%
TAGCAGCAGGAAGAATGATT+TGG + chr5_4:42623306-42623325 Msa0851950:intron 40.0%
ATCTCAGTGTTTCCAATCTG+AGG - chr5_4:42623358-42623377 None:intergenic 40.0%
! TGCGGCCAAGTATTTCTTTT+TGG - chr5_4:42623417-42623436 None:intergenic 40.0%
AGTTGTGATGAAAAAGGGAC+TGG - chr5_4:42623704-42623723 None:intergenic 40.0%
AAGTCTTTGCACTTGCTTCT+TGG - chr5_4:42624315-42624334 None:intergenic 40.0%
ATGTTCACAAGTAGGTTTGG+TGG - chr5_4:42624425-42624444 None:intergenic 40.0%
GGAAGAAATGGCAGTTTCAA+TGG - chr5_4:42624453-42624472 None:intergenic 40.0%
! AAACTGCCATTTCTTCCCAA+GGG + chr5_4:42624456-42624475 Msa0851950:CDS 40.0%
ATGCCGAATCTTGAAGTAGA+GGG - chr5_4:42624533-42624552 None:intergenic 40.0%
AATGCCGAATCTTGAAGTAG+AGG - chr5_4:42624534-42624553 None:intergenic 40.0%
GAACATTACATCGGCCATAT+CGG - chr5_4:42624570-42624589 None:intergenic 40.0%
! ACCCGAGTTGAACATTACAT+CGG - chr5_4:42624579-42624598 None:intergenic 40.0%
TAGTAGCAGTGGCAATAGTA+TGG + chr5_4:42624598-42624617 Msa0851950:CDS 40.0%
AGATAAAGGTGAACCTGATG+AGG + chr5_4:42624643-42624662 Msa0851950:CDS 40.0%
GAATAGCATTTCAGGGTGGT+TGG - chr5_4:42621287-42621306 None:intergenic 45.0%
CATGCTCAAATTGGTGGAAG+TGG + chr5_4:42621466-42621485 Msa0851950:CDS 45.0%
TAAGGTCTAGGGGAACTTTG+AGG + chr5_4:42621617-42621636 Msa0851950:CDS 45.0%
! TCTAGGGGAACTTTGAGGAT+TGG + chr5_4:42621622-42621641 Msa0851950:CDS 45.0%
! TTGAGGATTGGTGAAATGGG+TGG + chr5_4:42621634-42621653 Msa0851950:CDS 45.0%
! TGAGGATTGGTGAAATGGGT+GGG + chr5_4:42621635-42621654 Msa0851950:CDS 45.0%
GCTGTCTTGTGTGAATGATG+AGG - chr5_4:42622737-42622756 None:intergenic 45.0%
TGTTGTGCCTTCACATGCAT+TGG + chr5_4:42623283-42623302 Msa0851950:intron 45.0%
CAAGTAAAGCCATCTTCTGG+TGG - chr5_4:42623382-42623401 None:intergenic 45.0%
! CACCAGAAGATGGCTTTACT+TGG + chr5_4:42623380-42623399 Msa0851950:CDS 45.0%
CTCCAAGTAAAGCCATCTTC+TGG - chr5_4:42623385-42623404 None:intergenic 45.0%
GTGAGCTTAACTCAGTTGGT+AGG + chr5_4:42624111-42624130 Msa0851950:intron 45.0%
!! TTCACAAGTAGGTTTGGTGG+TGG - chr5_4:42624422-42624441 None:intergenic 45.0%
! GAAACTGCCATTTCTTCCCA+AGG + chr5_4:42624455-42624474 Msa0851950:CDS 45.0%
GGACCCTCTACTTCAAGATT+CGG + chr5_4:42624527-42624546 Msa0851950:CDS 45.0%
!! ATTCGGCATTGATTATCCGG+TGG + chr5_4:42624544-42624563 Msa0851950:CDS 45.0%
GGCCGATGTAATGTTCAACT+CGG + chr5_4:42624574-42624593 Msa0851950:CDS 45.0%
GCCGATGTAATGTTCAACTC+GGG + chr5_4:42624575-42624594 Msa0851950:CDS 45.0%
ATGATAAGTGGCGGTGGTGA+TGG + chr5_4:42621577-42621596 Msa0851950:CDS 50.0%
GCGGTGACGTTCAGTTGTTA+AGG + chr5_4:42621599-42621618 Msa0851950:CDS 50.0%
GATTGGTGAAATGGGTGGGA+GGG + chr5_4:42621639-42621658 Msa0851950:CDS 50.0%
TGAAATGGGTGGGAGGGATA+TGG + chr5_4:42621645-42621664 Msa0851950:CDS 50.0%
GCTGCTACCAATGCATGTGA+AGG - chr5_4:42623293-42623312 None:intergenic 50.0%
! TCACATGCATTGGTAGCAGC+AGG + chr5_4:42623293-42623312 Msa0851950:intron 50.0%
ATGCATCCAGCACCTCAGAT+TGG + chr5_4:42623343-42623362 Msa0851950:CDS 50.0%
! GTGTTTCCAATCTGAGGTGC+TGG - chr5_4:42623352-42623371 None:intergenic 50.0%
ACTGAGATGCCACCAGAAGA+TGG + chr5_4:42623370-42623389 Msa0851950:CDS 50.0%
CCAACTGAGTTAAGCTCACG+AGG - chr5_4:42624110-42624129 None:intergenic 50.0%
CCTCGTGAGCTTAACTCAGT+TGG + chr5_4:42624107-42624126 Msa0851950:intron 50.0%
CTTTGCACTTGCTTCTTGGC+TGG - chr5_4:42624311-42624330 None:intergenic 50.0%
!! GGTGGTGGTGGATATGATGA+TGG - chr5_4:42624407-42624426 None:intergenic 50.0%
!! ACAAGTAGGTTTGGTGGTGG+TGG - chr5_4:42624419-42624438 None:intergenic 50.0%
TGATTATCCGGTGGCCGATA+TGG + chr5_4:42624553-42624572 Msa0851950:CDS 50.0%
TTCAACTCGGGTAGTAGCAG+TGG + chr5_4:42624587-42624606 Msa0851950:CDS 50.0%
! TCAGGGTGGTTGGCCATGTT+GGG - chr5_4:42621277-42621296 None:intergenic 55.0%
! TTCAGGGTGGTTGGCCATGT+TGG - chr5_4:42621278-42621297 None:intergenic 55.0%
!! ATAAGTGGCGGTGGTGATGG+CGG + chr5_4:42621580-42621599 Msa0851950:CDS 55.0%
! GGATTGGTGAAATGGGTGGG+AGG + chr5_4:42621638-42621657 Msa0851950:CDS 55.0%
ATGGGTGGGAGGGATATGGA+AGG + chr5_4:42621649-42621668 Msa0851950:CDS 55.0%
TACTTGGCCGCAAGTACCCA+AGG + chr5_4:42623425-42623444 Msa0851950:CDS 55.0%
! CTGCCATTTCTTCCCAAGGG+TGG + chr5_4:42624459-42624478 Msa0851950:CDS 55.0%
TGGCCACCCTTGGGAAGAAA+TGG - chr5_4:42624465-42624484 None:intergenic 55.0%
GGGGTTCGAGGTTTGAACCC+CGG - chr5_4:42623089-42623108 None:intergenic 60.0%
GGGGTTCAAACCTCGAACCC+CGG + chr5_4:42623088-42623107 Msa0851950:intron 60.0%
!! TTGGTGATGACCGGGGTTCG+AGG - chr5_4:42623101-42623120 None:intergenic 60.0%
TGCGGTGAACCACGACCTCA+GGG + chr5_4:42624487-42624506 Msa0851950:CDS 60.0%
ACATCGGCCATATCGGCCAC+CGG - chr5_4:42624563-42624582 None:intergenic 60.0%
TTCCCAAGGGTGGCCACCTG+CGG + chr5_4:42624469-42624488 Msa0851950:CDS 65.0%
AGGTCGTGGTTCACCGCAGG+TGG - chr5_4:42624485-42624504 None:intergenic 65.0%
CTGAGGTCGTGGTTCACCGC+AGG - chr5_4:42624488-42624507 None:intergenic 65.0%
CTGCGGTGAACCACGACCTC+AGG + chr5_4:42624486-42624505 Msa0851950:CDS 65.0%
GCGGTGAACCACGACCTCAG+GGG + chr5_4:42624488-42624507 Msa0851950:CDS 65.0%
GTGAACCACGACCTCAGGGG+CGG + chr5_4:42624491-42624510 Msa0851950:CDS 65.0%
CACCGCAGGTGGCCACCCTT+GGG - chr5_4:42624474-42624493 None:intergenic 70.0%
TCACCGCAGGTGGCCACCCT+TGG - chr5_4:42624475-42624494 None:intergenic 70.0%
CACGACCTCAGGGGCGGAGA+CGG + chr5_4:42624497-42624516 Msa0851950:CDS 70.0%
CAGTACCGTCTCCGCCCCTG+AGG - chr5_4:42624505-42624524 None:intergenic 70.0%
CTCAGGGGCGGAGACGGTAC+TGG + chr5_4:42624503-42624522 Msa0851950:CDS 70.0%
! AGGGGCGGAGACGGTACTGG+TGG + chr5_4:42624506-42624525 Msa0851950:CDS 70.0%
CGTCTCCGCCCCTGAGGTCG+TGG - chr5_4:42624499-42624518 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 42621208 42624676 42621208 ID=Msa0851950;Name=Msa0851950
chr5_4 mRNA 42621208 42624676 42621208 ID=Msa0851950-mRNA-1;Parent=Msa0851950;Name=Msa0851950-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|361
chr5_4 exon 42621208 42621746 42621208 ID=Msa0851950-mRNA-1:exon:13500;Parent=Msa0851950-mRNA-1
chr5_4 exon 42623309 42623446 42623309 ID=Msa0851950-mRNA-1:exon:13501;Parent=Msa0851950-mRNA-1
chr5_4 exon 42624268 42624676 42624268 ID=Msa0851950-mRNA-1:exon:13502;Parent=Msa0851950-mRNA-1
chr5_4 CDS 42621208 42621746 42621208 ID=Msa0851950-mRNA-1:cds;Parent=Msa0851950-mRNA-1
chr5_4 CDS 42623309 42623446 42623309 ID=Msa0851950-mRNA-1:cds;Parent=Msa0851950-mRNA-1
chr5_4 CDS 42624268 42624676 42624268 ID=Msa0851950-mRNA-1:cds;Parent=Msa0851950-mRNA-1
Gene Sequence

>Msa0851950

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGTTGTCATCACAACATGACACAACAACAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGGAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATATGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGTACCCAAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCCTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>Msa0851950

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMLSSQHDTTTTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRGTLRIGEMGGRDMEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKYPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN*