AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0735320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735320 MtrunA17_Chr5g0432441 89.264 326 10 2 1 301 1 326 0.0 576
Msa0735320 MtrunA17_Chr3g0106921 65.409 318 84 3 9 301 4 320 6.91e-145 410
Msa0735320 MtrunA17_Chr3g0129051 59.077 325 101 6 3 301 4 322 4.29e-125 360
Msa0735320 MtrunA17_Chr5g0438021 58.095 315 105 4 12 301 13 325 4.85e-120 347
Msa0735320 MtrunA17_Chr4g0013171 56.347 323 112 5 5 301 2 321 5.41e-114 332
Msa0735320 MtrunA17_Chr3g0129061 58.199 311 102 5 17 301 14 322 1.38e-110 323
Msa0735320 MtrunA17_Chr4g0013201 54.667 300 107 4 27 301 43 338 7.87e-109 319
Msa0735320 MtrunA17_Chr4g0013191 53.896 308 110 5 22 301 37 340 1.95e-108 318
Msa0735320 MtrunA17_Chr3g0129041 58.521 311 101 6 17 301 14 322 8.62e-108 316
Msa0735320 MtrunA17_Chr4g0013181 57.627 295 96 4 33 301 62 353 5.87e-106 312
Msa0735320 MtrunA17_Chr3g0114451 53.774 318 122 4 8 301 2 318 1.11e-105 310
Msa0735320 MtrunA17_Chr7g0244461 54.515 299 107 5 28 301 18 312 9.53e-105 308
Msa0735320 MtrunA17_Chr4g0038161 50.955 314 125 4 15 301 9 320 1.21e-103 305
Msa0735320 MtrunA17_Chr4g0038151 52.492 301 114 4 28 301 23 321 2.68e-103 305
Msa0735320 MtrunA17_Chr4g0043391 51.899 316 120 6 15 301 12 324 3.09e-103 304
Msa0735320 MtrunA17_Chr2g0320071 52.885 312 117 6 16 301 10 317 1.35e-102 303
Msa0735320 MtrunA17_Chr7g0244451 54.839 310 111 5 17 301 11 316 2.42e-102 302
Msa0735320 MtrunA17_Chr4g0038171 50.000 324 127 7 7 301 4 321 4.23e-102 301
Msa0735320 MtrunA17_Chr5g0432341 51.242 322 122 8 10 301 5 321 6.88e-101 298
Msa0735320 MtrunA17_Chr5g0432351 52.174 322 119 10 10 301 5 321 1.59e-100 297
Msa0735320 MtrunA17_Chr3g0106901 53.846 325 115 7 7 301 4 323 2.80e-100 297
Msa0735320 MtrunA17_Chr2g0320101 52.843 299 112 5 28 301 23 317 8.52e-100 295
Msa0735320 MtrunA17_Chr5g0432331 50.000 326 126 9 6 301 34 352 3.11e-98 293
Msa0735320 MtrunA17_Chr2g0320091 51.438 313 123 6 15 301 9 318 5.06e-98 291
Msa0735320 MtrunA17_Chr1g0213061 48.936 329 132 8 1 301 1 321 1.75e-96 287
Msa0735320 MtrunA17_Chr3g0107881 49.068 322 126 7 12 301 9 324 4.39e-96 286
Msa0735320 MtrunA17_Chr2g0320061 50.625 320 127 7 9 301 3 318 2.02e-95 284
Msa0735320 MtrunA17_Chr5g0432401 53.289 304 117 8 1 299 1 284 5.76e-95 282
Msa0735320 MtrunA17_Chr2g0320031 49.842 317 127 6 13 301 7 319 3.55e-93 279
Msa0735320 MtrunA17_Chr2g0320051 51.333 300 116 6 28 301 23 318 6.60e-93 278
Msa0735320 MtrunA17_Chr7g0224371 48.624 327 137 6 1 301 1 322 1.18e-92 277
Msa0735320 MtrunA17_Chr5g0402571 47.649 319 135 4 13 301 10 326 8.21e-91 273
Msa0735320 MtrunA17_Chr5g0402581 46.417 321 136 3 13 301 10 326 2.46e-90 271
Msa0735320 MtrunA17_Chr1g0175561 46.707 334 134 9 3 301 2 326 2.55e-88 266
Msa0735320 MtrunA17_Chr5g0404721 49.180 305 118 6 30 301 30 330 1.49e-87 265
Msa0735320 MtrunA17_Chr7g0258811 45.511 323 135 7 15 301 54 371 1.84e-86 263
Msa0735320 MtrunA17_Chr4g0070951 48.297 323 130 7 13 301 10 329 4.14e-86 261
Msa0735320 MtrunA17_Chr2g0292101 45.455 319 136 5 17 301 12 326 1.04e-84 258
Msa0735320 MtrunA17_Chr2g0299691 46.334 341 138 6 3 301 21 358 4.90e-84 257
Msa0735320 MtrunA17_Chr2g0292091 44.479 326 145 4 9 301 3 325 5.40e-84 256
Msa0735320 MtrunA17_Chr1g0155611 48.366 306 120 5 30 301 46 347 1.20e-83 255
Msa0735320 MtrunA17_Chr4g0068621 47.682 302 129 4 28 301 36 336 1.49e-83 254
Msa0735320 MtrunA17_Chr8g0372601 47.883 307 123 6 28 301 28 330 2.24e-83 254
Msa0735320 MtrunA17_Chr2g0292251 46.885 305 130 3 28 301 26 329 1.73e-82 253
Msa0735320 MtrunA17_Chr2g0292241 45.231 325 146 4 8 301 7 330 6.60e-81 248
Msa0735320 MtrunA17_Chr2g0292211 44.860 321 144 5 13 301 22 341 9.33e-81 248
Msa0735320 MtrunA17_Chr2g0292151 45.482 332 143 5 4 301 3 330 3.05e-80 247
Msa0735320 MtrunA17_Chr2g0292181 45.455 319 142 4 14 301 22 339 7.72e-80 246
Msa0735320 MtrunA17_Chr2g0291751 44.611 334 146 6 3 301 1 330 2.20e-79 244
Msa0735320 MtrunA17_Chr2g0292111 45.509 334 143 8 3 301 1 330 2.09e-78 242
Msa0735320 MtrunA17_Chr5g0400331 46.580 307 128 5 30 301 26 331 1.45e-77 239
Msa0735320 MtrunA17_Chr4g0013721 43.506 308 141 5 23 301 93 396 3.70e-77 241
Msa0735320 MtrunA17_Chr5g0421411 44.983 289 118 6 50 301 1 285 6.39e-77 236
Msa0735320 MtrunA17_Chr2g0292271 43.902 328 147 7 8 301 6 330 5.42e-76 236
Msa0735320 MtrunA17_Chr4g0013821 42.395 309 133 7 28 301 26 324 1.63e-74 232
Msa0735320 MtrunA17_Chr2g0292261 43.519 324 141 7 14 301 13 330 2.26e-73 229
Msa0735320 MtrunA17_Chr2g0292291 41.925 322 150 6 14 301 14 332 1.09e-72 228
Msa0735320 MtrunA17_Chr6g0477071 40.317 315 163 5 8 301 32 342 5.98e-71 223
Msa0735320 MtrunA17_Chr1g0188431 37.764 331 172 5 1 301 1 327 1.19e-69 219
Msa0735320 MtrunA17_Chr4g0024121 37.342 316 166 4 12 297 9 322 2.49e-69 218
Msa0735320 MtrunA17_Chr2g0322861 40.379 317 155 6 16 301 73 386 6.51e-69 219
Msa0735320 MtrunA17_Chr1g0194151 37.798 336 171 4 1 301 1 333 8.62e-67 212
Msa0735320 MtrunA17_Chr1g0164891 41.196 301 143 5 34 301 93 392 3.32e-65 209
Msa0735320 MtrunA17_Chr1g0204011 37.695 321 164 7 11 301 8 322 8.61e-65 206
Msa0735320 MtrunA17_Chr2g0279041 39.385 325 150 10 15 301 10 325 2.94e-64 205
Msa0735320 MtrunA17_Chr1g0194271 36.527 334 171 4 8 301 8 340 1.21e-63 204
Msa0735320 MtrunA17_Chr7g0225221 40.000 325 158 8 7 301 3 320 2.09e-63 202
Msa0735320 MtrunA17_Chr6g0460711 37.179 312 166 5 16 301 7 314 5.77e-63 201
Msa0735320 MtrunA17_Chr3g0128061 38.272 324 165 4 10 301 6 326 5.96e-63 201
Msa0735320 MtrunA17_Chr1g0157051 39.683 315 151 8 15 297 7 314 1.48e-62 200
Msa0735320 MtrunA17_Chr4g0046131 40.058 347 153 9 1 297 1 342 1.74e-61 198
Msa0735320 MtrunA17_Chr5g0405991 36.957 322 151 6 17 301 10 316 5.39e-61 196
Msa0735320 MtrunA17_Chr6g0467161 40.604 298 145 6 30 301 45 336 1.14e-60 196
Msa0735320 MtrunA17_Chr7g0254201 36.577 298 160 4 26 298 16 309 1.77e-60 195
Msa0735320 MtrunA17_Chr4g0076211 38.435 294 148 6 35 301 46 333 1.19e-59 193
Msa0735320 MtrunA17_Chr4g0063671 38.585 311 145 8 28 301 19 320 1.37e-59 192
Msa0735320 MtrunA17_Chr2g0296621 37.793 299 152 6 30 301 50 341 4.12e-58 189
Msa0735320 MtrunA17_Chr8g0371381 38.079 302 149 7 30 301 26 319 5.03e-58 189
Msa0735320 MtrunA17_Chr7g0268501 37.025 316 164 7 15 301 11 320 4.77e-57 186
Msa0735320 MtrunA17_Chr7g0268521 37.025 316 164 7 15 301 11 320 4.77e-57 186
Msa0735320 MtrunA17_Chr7g0254231 35.739 291 161 4 30 298 22 308 1.29e-56 185
Msa0735320 MtrunA17_Chr6g0451571 35.938 320 170 7 15 301 17 334 5.12e-56 184
Msa0735320 MtrunA17_Chr6g0467251 35.061 328 178 6 1 297 1 324 5.89e-55 182
Msa0735320 MtrunA17_Chr2g0278671 38.782 312 153 8 23 301 17 323 1.19e-54 180
Msa0735320 MtrunA17_Chr8g0364291 36.250 320 172 4 13 301 6 324 1.32e-54 180
Msa0735320 MtrunA17_Chr1g0197411 34.043 329 178 7 11 301 3 330 2.34e-53 177
Msa0735320 MtrunA17_Chr4g0053871 39.535 301 150 8 26 297 23 320 7.28e-53 176
Msa0735320 MtrunA17_Chr4g0072091 38.235 306 151 7 29 301 23 323 5.62e-52 173
Msa0735320 MtrunA17_Chr4g0074851 33.645 321 182 6 10 301 10 328 3.80e-51 171
Msa0735320 MtrunA17_Chr2g0279061 41.154 260 108 8 74 297 12 262 9.75e-51 168
Msa0735320 MtrunA17_Chr2g0316831 35.736 333 151 10 28 299 110 440 2.04e-50 172
Msa0735320 MtrunA17_Chr6g0467121 36.149 296 159 5 30 301 74 363 4.13e-50 170
Msa0735320 MtrunA17_Chr5g0401431 34.713 314 170 6 17 297 14 325 5.74e-50 168
Msa0735320 MtrunA17_Chr4g0075241 32.226 301 175 3 29 301 28 327 7.71e-50 168
Msa0735320 MtrunA17_Chr2g0316791 34.234 333 156 7 28 299 74 404 9.22e-50 170
Msa0735320 MtrunA17_Chr2g0316481 34.535 333 155 8 28 299 74 404 2.10e-49 169
Msa0735320 MtrunA17_Chr5g0413051 33.550 307 169 5 29 301 72 377 5.79e-49 167
Msa0735320 MtrunA17_Chr1g0181431 34.106 302 170 5 27 301 24 323 6.74e-49 165
Msa0735320 MtrunA17_Chr1g0181481 35.216 301 166 5 28 301 25 323 9.48e-49 165
Msa0735320 MtrunA17_Chr1g0148021 33.639 327 182 6 9 301 3 328 1.47e-48 164
Msa0735320 MtrunA17_Chr1g0181511 34.884 301 167 7 28 301 25 323 2.78e-47 161
Msa0735320 MtrunA17_Chr2g0311251 33.832 334 157 11 28 300 9 339 4.48e-47 161
Msa0735320 MtrunA17_Chr3g0114461 47.090 189 76 3 136 301 5 192 2.13e-46 155
Msa0735320 MtrunA17_Chr3g0136751 31.988 322 183 5 11 301 6 322 4.26e-46 158
Msa0735320 MtrunA17_Chr2g0329641 33.225 307 165 6 29 301 35 335 3.97e-45 155
Msa0735320 MtrunA17_Chr3g0114491 62.500 112 42 0 29 140 17 128 5.53e-44 147
Msa0735320 MtrunA17_Chr2g0316811 44.571 175 93 3 28 200 48 220 2.31e-42 148
Msa0735320 MtrunA17_Chr2g0316191 45.614 171 89 3 28 196 74 242 1.64e-40 145
Msa0735320 MtrunA17_Chr2g0316141 35.448 268 127 10 76 299 11 276 5.55e-39 138
Msa0735320 MtrunA17_Chr2g0316181 35.448 268 127 10 76 299 11 276 5.55e-39 138
Msa0735320 MtrunA17_Chr2g0311241 29.867 375 158 12 28 300 58 429 1.06e-36 135
Msa0735320 MtrunA17_Chr4g0004591 28.125 320 140 5 30 300 77 355 2.11e-35 130
Msa0735320 MtrunA17_Chr2g0292281 41.294 201 96 5 122 301 17 216 1.47e-33 123
Msa0735320 MtrunA17_Chr5g0432361 53.191 94 41 2 209 300 16 108 2.28e-26 100
Msa0735320 MtrunA17_Chr6g0468731 65.714 70 20 2 69 138 22 87 3.25e-23 91.3
Msa0735320 MtrunA17_Chr1g0181501 32.105 190 102 3 138 301 1 189 1.58e-22 92.4
Msa0735320 MtrunA17_Chr3g0137751 28.571 238 120 7 64 283 108 313 5.86e-18 82.8
Msa0735320 MtrunA17_Chr1g0181491 38.182 110 66 2 28 137 10 117 1.44e-17 78.2
Msa0735320 MtrunA17_Chr7g0254181 42.268 97 52 2 100 196 29 121 8.25e-17 76.3
Msa0735320 MtrunA17_Chr3g0104101 41.346 104 49 4 205 301 51 149 3.95e-16 74.3
Msa0735320 MtrunA17_Chr1g0196191 54.286 70 31 1 68 137 23 91 8.65e-16 73.2
Msa0735320 MtrunA17_Chr5g0432321 42.149 121 40 6 151 246 17 132 4.77e-14 67.8
Msa0735320 MtrunA17_Chr5g0423911 41.791 67 39 0 231 297 1 67 1.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735320 AT5G58390.1 61.218 312 95 4 15 301 6 316 6.98e-121 349
Msa0735320 AT5G58400.1 56.369 314 111 2 13 301 13 325 1.20e-118 344
Msa0735320 AT5G05340.1 58.497 306 97 5 21 301 24 324 1.66e-117 341
Msa0735320 AT1G14550.1 51.104 317 122 5 15 301 8 321 1.11e-99 296
Msa0735320 AT1G14540.1 47.335 319 137 5 10 301 1 315 1.83e-89 270
Msa0735320 AT5G06730.1 44.048 336 151 6 1 301 1 334 2.86e-89 270
Msa0735320 AT5G06720.1 45.342 322 140 5 15 301 13 333 1.18e-88 268
Msa0735320 AT4G36430.1 46.749 323 132 6 13 301 14 330 2.15e-88 267
Msa0735320 AT3G50990.1 47.896 309 124 7 26 301 37 341 3.25e-86 262
Msa0735320 AT5G66390.1 46.129 310 130 4 25 301 28 333 3.63e-86 262
Msa0735320 AT1G44970.1 44.571 350 139 7 3 301 1 346 3.89e-86 262
Msa0735320 AT2G18150.1 44.910 334 135 6 13 301 7 336 6.94e-86 261
Msa0735320 AT4G16270.1 49.664 298 117 6 34 301 54 348 7.42e-85 259
Msa0735320 AT1G49570.1 46.580 307 127 4 25 297 43 346 2.04e-81 250
Msa0735320 AT5G19890.1 45.768 319 133 5 16 301 16 327 2.74e-80 246
Msa0735320 AT2G18140.1 45.695 302 127 4 33 301 38 335 3.23e-80 246
Msa0735320 AT4G08770.1 45.397 315 126 7 26 301 20 327 3.27e-80 247
Msa0735320 AT2G38390.1 44.839 310 130 7 28 301 29 333 6.89e-79 243
Msa0735320 AT4G08780.1 44.444 315 129 7 26 301 20 327 2.14e-78 242
Msa0735320 AT5G19880.1 44.099 322 137 6 17 301 14 329 3.02e-78 241
Msa0735320 AT2G35380.1 43.402 341 147 7 1 301 1 335 5.51e-78 241
Msa0735320 AT2G38380.1 44.839 310 130 7 28 301 29 333 2.65e-77 239
Msa0735320 AT1G68850.1 40.663 332 150 7 15 301 6 335 3.75e-77 239
Msa0735320 AT3G32980.1 44.268 314 131 8 26 301 27 334 2.03e-76 237
Msa0735320 AT3G49110.1 45.079 315 137 7 22 301 23 336 3.39e-76 237
Msa0735320 AT3G49120.1 44.337 309 138 6 26 301 28 335 8.33e-76 236
Msa0735320 AT3G01190.1 40.635 315 152 7 17 301 12 321 1.18e-71 224
Msa0735320 AT2G22420.1 42.333 300 142 4 30 301 23 319 2.36e-71 224
Msa0735320 AT3G03670.1 37.736 318 167 5 13 301 6 321 3.52e-68 215
Msa0735320 AT1G71695.1 40.741 297 150 3 30 301 44 339 2.04e-67 214
Msa0735320 AT4G11290.1 38.154 325 163 7 13 301 4 326 3.53e-67 213
Msa0735320 AT4G33420.1 39.432 317 165 5 8 301 13 325 6.27e-67 212
Msa0735320 AT1G05260.1 39.241 316 158 6 17 301 13 325 1.90e-66 211
Msa0735320 AT4G33420.2 39.432 317 165 5 8 301 60 372 2.64e-66 212
Msa0735320 AT5G17820.1 37.580 314 164 5 13 301 7 313 2.41e-65 207
Msa0735320 AT5G17820.2 37.580 314 164 5 13 301 60 366 3.02e-64 206
Msa0735320 AT5G51890.1 36.760 321 164 6 13 301 9 322 1.32e-63 203
Msa0735320 AT5G15180.1 35.843 332 177 7 3 301 1 329 6.94e-63 202
Msa0735320 AT5G40150.1 37.171 304 156 6 28 301 30 328 8.84e-62 199
Msa0735320 AT4G26010.1 38.158 304 163 5 15 298 8 306 1.26e-61 198
Msa0735320 AT4G26010.2 36.825 315 174 5 5 298 24 334 1.40e-61 199
Msa0735320 AT3G21770.1 37.061 313 163 6 19 300 18 327 1.73e-61 198
Msa0735320 AT3G28200.1 36.304 303 159 5 28 301 19 316 4.29e-61 197
Msa0735320 AT5G42180.1 38.245 319 159 7 10 299 6 315 1.14e-60 196
Msa0735320 AT2G41480.1 37.313 335 160 11 8 301 3 328 1.23e-60 196
Msa0735320 AT5G14130.1 37.261 314 166 4 17 301 19 330 1.25e-60 196
Msa0735320 AT5G14130.2 37.419 310 163 4 17 297 60 367 4.80e-60 196
Msa0735320 AT1G05240.1 38.333 300 149 7 30 297 26 321 4.96e-60 194
Msa0735320 AT1G05250.1 38.333 300 149 7 30 297 26 321 4.96e-60 194
Msa0735320 AT1G77100.1 36.335 322 173 8 8 301 2 319 4.88e-59 192
Msa0735320 AT5G64120.1 36.246 309 155 7 25 301 30 328 3.41e-58 190
Msa0735320 AT4G17690.1 38.796 299 154 5 30 301 26 322 8.31e-58 189
Msa0735320 AT5G47000.1 37.662 308 162 5 22 301 25 330 8.51e-58 189
Msa0735320 AT1G34510.1 37.582 306 166 6 17 301 8 309 1.07e-56 185
Msa0735320 AT2G24800.1 36.601 306 157 4 28 301 29 329 1.54e-55 183
Msa0735320 AT2G35380.2 44.490 245 100 4 92 301 4 247 1.61e-55 180
Msa0735320 AT5G39580.1 36.562 320 168 5 11 301 6 319 2.49e-55 182
Msa0735320 AT1G30870.1 38.033 305 146 6 30 301 51 345 2.28e-54 181
Msa0735320 AT4G31760.1 33.962 318 173 5 15 301 15 326 3.46e-54 179
Msa0735320 AT4G37530.1 38.585 311 150 9 23 297 20 325 2.31e-53 177
Msa0735320 AT5G67400.1 37.097 310 154 7 24 297 21 325 3.25e-52 174
Msa0735320 AT2G18980.1 35.494 324 170 7 11 301 6 323 5.06e-52 174
Msa0735320 AT1G24110.1 34.754 305 166 6 28 301 20 322 2.60e-51 172
Msa0735320 AT3G49960.1 35.849 318 167 8 13 297 12 325 4.41e-51 171
Msa0735320 AT4G30170.1 36.808 307 155 7 28 301 25 325 4.92e-51 171
Msa0735320 AT4G37520.1 37.954 303 153 7 28 297 25 325 5.01e-51 171
Msa0735320 AT5G64100.1 35.644 303 148 7 34 301 41 331 8.34e-51 171
Msa0735320 AT5G64110.1 36.890 328 160 8 14 301 10 330 9.05e-51 171
Msa0735320 AT4G25980.1 34.713 314 158 9 26 301 22 326 4.51e-50 169
Msa0735320 AT4G21960.1 36.455 299 161 5 30 301 31 327 4.78e-50 169
Msa0735320 AT4G37520.2 36.755 302 155 6 28 297 25 322 1.15e-49 167
Msa0735320 AT2G34060.1 36.393 305 157 6 29 298 41 343 1.61e-48 165
Msa0735320 AT2G37130.1 32.911 316 169 10 19 301 22 327 2.06e-44 154
Msa0735320 AT2G39040.1 35.275 309 161 7 29 301 45 350 6.23e-44 153
Msa0735320 AT2G37130.2 33.444 302 161 9 33 301 2 296 6.25e-44 152
Msa0735320 AT5G22410.1 33.731 335 172 11 1 298 1 322 8.61e-44 152
Msa0735320 AT4G33870.1 42.105 190 106 3 18 205 54 241 9.14e-44 154
Msa0735320 AT4G37530.2 39.394 264 119 9 23 250 20 278 9.32e-44 151
Msa0735320 AT2G24800.2 46.286 175 89 2 28 202 29 198 1.24e-43 149
Msa0735320 AT5G24070.1 35.662 272 134 6 64 301 71 335 5.14e-42 148
Msa0735320 AT5G39580.2 43.548 186 99 3 11 196 6 185 8.21e-41 142
Msa0735320 AT4G25980.2 41.714 175 97 3 26 200 67 236 9.92e-41 143
Msa0735320 AT2G43480.1 32.372 312 168 7 26 301 31 335 1.18e-39 142
Msa0735320 AT3G17070.1 29.967 307 175 6 30 301 38 339 1.19e-39 142
Msa0735320 AT3G17070.2 26.882 279 163 7 58 301 4 276 1.66e-26 105
Msa0735320 AT4G32320.1 30.705 241 117 7 61 283 114 322 7.53e-19 85.9
Msa0735320 AT3G42570.1 31.092 119 80 1 83 199 32 150 5.80e-13 65.9

Find 64 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTCCACCTTAGACTTTA+TGG 0.119850 5_1:-50505341 None:intergenic
CTACCCAGGTTTCCTTTAAT+TGG 0.214761 5_1:-50507846 None:intergenic
CAAGAATCTCATTAACAAAA+AGG 0.243097 5_1:+50507646 Msa0735320:CDS
TGCAGCTTGGAGGGCCATTT+TGG 0.267352 5_1:+50506603 Msa0735320:intron
TTTGTCAAGTCTTTAGGATT+AGG 0.272946 5_1:+50503343 None:intergenic
CCAAGTTTAACCTTCCAAAA+TGG 0.296962 5_1:-50506617 None:intergenic
TGTAGTATAAGTTATCAAAA+TGG 0.316765 5_1:-50507625 None:intergenic
AAGGACATGGTGGCCTTATC+TGG 0.324620 5_1:+50506755 Msa0735320:CDS
TTGCTTCCTAATCTCACCTT+TGG 0.333452 5_1:-50507813 None:intergenic
GGAAATAACAAGCCTCTTAC+CGG 0.340680 5_1:+50507788 Msa0735320:CDS
AATGCAGGAGAGCCAATTAA+AGG 0.356166 5_1:+50507834 Msa0735320:CDS
CATTATATATAGAGATAATA+TGG 0.375975 5_1:-50503381 None:intergenic
TATACTACTACTTAGCAAAA+TGG 0.382816 5_1:+50503446 Msa0735320:exon
CCCTTAGCAACCTTATCAAT+AGG 0.384569 5_1:+50506708 Msa0735320:CDS
AGTCTTCTCTCCTTTATTAG+AGG 0.384702 5_1:-50505272 None:intergenic
AAGGTTGCTAAGGGTAGAAA+AGG 0.387632 5_1:-50506699 None:intergenic
TGCTACTGCAATGATTAAGA+TGG 0.388563 5_1:+50507766 Msa0735320:CDS
TGGAGGATCTACTGATTCAT+TGG 0.391305 5_1:+50507700 Msa0735320:CDS
GAGCTTTAAACCTATTGATA+AGG 0.399870 5_1:-50506718 None:intergenic
TATTGATGCCATAAAGTCTA+AGG 0.403057 5_1:+50505333 Msa0735320:CDS
GATGACACACCCTCTAATAA+AGG 0.415165 5_1:+50505262 Msa0735320:CDS
ACCTATTGATAAGGTTGCTA+AGG 0.417064 5_1:-50506709 None:intergenic
TCACCTTTGGATCCGGTAAG+AGG 0.425804 5_1:-50507800 None:intergenic
ACCTCTCAAAGAATCTTTGT+TGG 0.427591 5_1:-50505302 None:intergenic
CCTAATCTCACCTTTGGATC+CGG 0.432355 5_1:-50507807 None:intergenic
CCTCTCAAAGAATCTTTGTT+GGG 0.433102 5_1:-50505301 None:intergenic
GGAAGGTTAAACTTGGAAGA+AGG 0.441433 5_1:+50506624 Msa0735320:CDS
GTCTTCTCTCCTTTATTAGA+GGG 0.447683 5_1:-50505271 None:intergenic
TAGTATAAGGCTTTAGAAGA+TGG 0.451638 5_1:-50503430 None:intergenic
CCGGATCCAAAGGTGAGATT+AGG 0.455777 5_1:+50507807 Msa0735320:CDS
GAAGGTTAAACTTGGAAGAA+GGG 0.455829 5_1:+50506625 Msa0735320:CDS
TATAAGTTATCAAAATGGTT+TGG 0.466239 5_1:-50507620 None:intergenic
ATCAATAGGTTTAAAGCTCA+AGG 0.476560 5_1:+50506722 Msa0735320:CDS
ACTGTTTGCATCATTGAGGC+TGG 0.487052 5_1:-50506660 None:intergenic
GCTACTGCAATGATTAAGAT+GGG 0.493974 5_1:+50507767 Msa0735320:CDS
CCCAACAAAGATTCTTTGAG+AGG 0.507682 5_1:+50505301 Msa0735320:CDS
GTGAGATTAGGAAGCAATGC+AGG 0.517002 5_1:+50507819 Msa0735320:CDS
TCAGCACATGAGACCACACC+GGG 0.520500 5_1:-50505370 None:intergenic
CACCACTGTTTGCATCATTG+AGG 0.529112 5_1:-50506664 None:intergenic
AGCTCAAGGACTCTCTACTA+AGG 0.542515 5_1:+50506736 Msa0735320:CDS
CCTATTGATAAGGTTGCTAA+GGG 0.554792 5_1:-50506708 None:intergenic
AACAGAATCACGAGCAGCAA+TGG 0.556484 5_1:-50505401 None:intergenic
AAAGCTCTTGATCAGAATGA+AGG 0.559330 5_1:-50507673 None:intergenic
AGGAATTGTCCAAGGAAAAG+TGG 0.565194 5_1:+50507554 Msa0735320:CDS
ATAAGGCTTTAGAAGATGGA+TGG 0.567520 5_1:-50503426 None:intergenic
AGGTTGCTAAGGGTAGAAAA+GGG 0.568432 5_1:-50506698 None:intergenic
CTAAGGGTAGAAAAGGGAGG+TGG 0.578099 5_1:-50506692 None:intergenic
AAGCCTCTTACCGGATCCAA+AGG 0.591242 5_1:+50507797 Msa0735320:CDS
CAAGGCAGAGGAATTGTCCA+AGG 0.617740 5_1:+50507546 Msa0735320:intron
GTCGCTAGAGAACCTCGCAT+GGG 0.619429 5_1:+50503624 Msa0735320:CDS
TTGCTAAGGGTAGAAAAGGG+AGG 0.621073 5_1:-50506695 None:intergenic
TGTCGCTAGAGAACCTCGCA+TGG 0.621286 5_1:+50503623 Msa0735320:CDS
TGATGCCATAAAGTCTAAGG+TGG 0.622773 5_1:+50505336 Msa0735320:CDS
GCTAGAGAACCTCGCATGGG+AGG 0.623031 5_1:+50503627 Msa0735320:CDS
AAGGTGGAGTCAGTATGCCC+CGG 0.647987 5_1:+50505352 Msa0735320:CDS
AGGAGAGATCCTCCCATGCG+AGG 0.655274 5_1:-50503636 None:intergenic
GGAGTCAGTATGCCCCGGTG+TGG 0.661070 5_1:+50505357 Msa0735320:CDS
ATCAGCACATGAGACCACAC+CGG 0.662563 5_1:-50505371 None:intergenic
AGGACTCTCTACTAAGGACA+TGG 0.666154 5_1:+50506742 Msa0735320:CDS
AGTCATGAAAATGAAGACGA+AGG 0.684805 5_1:-50503656 None:intergenic
AGCCTCAATGATGCAAACAG+TGG 0.715380 5_1:+50506662 Msa0735320:CDS
ACTCTCTACTAAGGACATGG+TGG 0.722310 5_1:+50506745 Msa0735320:CDS
AGCGACAGCAGAGTGAACAA+CGG 0.726357 5_1:-50503607 None:intergenic
CAGCACATGAGACCACACCG+GGG 0.839989 5_1:-50505369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTGAATATATTATTTTT+TGG + chr5_1:50504224-50504243 Msa0735320:intron 10.0%
!! ATTTATTAGATAAAGATTAA+GGG - chr5_1:50504736-50504755 None:intergenic 10.0%
!! AATTTATTAGATAAAGATTA+AGG - chr5_1:50504737-50504756 None:intergenic 10.0%
!! TAATCTTTATCTAATAAATT+TGG + chr5_1:50504737-50504756 Msa0735320:intron 10.0%
!!! AAATTGATATCTAATTATTT+TGG + chr5_1:50505539-50505558 Msa0735320:intron 10.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr5_1:50506230-50506249 Msa0735320:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + chr5_1:50506231-50506250 Msa0735320:intron 10.0%
!! CATTATATATAGAGATAATA+TGG - chr5_1:50503384-50503403 None:intergenic 15.0%
!! TTGGTTACTAAATAATAATA+TGG - chr5_1:50504152-50504171 None:intergenic 15.0%
!!! ACACAAATTTAGTAATTTTT+TGG - chr5_1:50504560-50504579 None:intergenic 15.0%
!! AAAAATTACTAAATTTGTGT+CGG + chr5_1:50504560-50504579 Msa0735320:intron 15.0%
!! AATTTAGTACATATTCAAAT+AGG - chr5_1:50504611-50504630 None:intergenic 15.0%
!! TTTATTAGATAAAGATTAAG+GGG - chr5_1:50504735-50504754 None:intergenic 15.0%
!!! TTAGTTTATTTTTATTGAAG+AGG + chr5_1:50505817-50505836 Msa0735320:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr5_1:50506232-50506251 Msa0735320:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr5_1:50506295-50506314 Msa0735320:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr5_1:50506296-50506315 Msa0735320:intron 15.0%
!! ATGTTTAAATTAGATACAAT+AGG - chr5_1:50506794-50506813 None:intergenic 15.0%
!!! AATAATTAAATGTGTTTTCT+TGG - chr5_1:50507049-50507068 None:intergenic 15.0%
!! AAGAAAACACATTTAATTAT+TGG + chr5_1:50507048-50507067 Msa0735320:intron 15.0%
!! TGTTGAAAATGAAATTTAAA+CGG - chr5_1:50507117-50507136 None:intergenic 15.0%
!!! TTCATGTTTTATCTTATAGA+TGG + chr5_1:50503516-50503535 Msa0735320:CDS 20.0%
!! AATATTGAGTGAATTGAATA+GGG + chr5_1:50504281-50504300 Msa0735320:intron 20.0%
!! AATATGTACTAAATTATGCA+TGG + chr5_1:50504616-50504635 Msa0735320:intron 20.0%
!!! ACTTTTAAATTCTTGAGATA+GGG + chr5_1:50504645-50504664 Msa0735320:intron 20.0%
!! ATTATCTGATATGCTAAATA+TGG - chr5_1:50505076-50505095 None:intergenic 20.0%
!!! TAAATCACTTTTTTCTAAAC+AGG + chr5_1:50505216-50505235 Msa0735320:intron 20.0%
!!! AAATCACTTTTTTCTAAACA+GGG + chr5_1:50505217-50505236 Msa0735320:intron 20.0%
!!! GTTCATTATTAAACTTTTTC+TGG - chr5_1:50505497-50505516 None:intergenic 20.0%
!!! TTATTTTGGTGTTTGTAAAA+TGG + chr5_1:50505553-50505572 Msa0735320:intron 20.0%
!!! TATTTTGGTGTTTGTAAAAT+GGG + chr5_1:50505554-50505573 Msa0735320:intron 20.0%
!! AATATAATGTTTACAAGTCT+TGG + chr5_1:50505640-50505659 Msa0735320:intron 20.0%
!! AATATGTGCATATAAATTGT+TGG - chr5_1:50505799-50505818 None:intergenic 20.0%
!!! TTTATTGAAGAGGTTTTTAA+TGG + chr5_1:50505827-50505846 Msa0735320:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr5_1:50506255-50506274 Msa0735320:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr5_1:50506365-50506384 Msa0735320:intron 20.0%
!! TATTAGAAGAAAAGAAATGA+GGG - chr5_1:50506522-50506541 None:intergenic 20.0%
!! TTATTAGAAGAAAAGAAATG+AGG - chr5_1:50506523-50506542 None:intergenic 20.0%
!!! GTTACTATTCATTTTAACTT+TGG - chr5_1:50507093-50507112 None:intergenic 20.0%
!! TATAAGTTATCAAAATGGTT+TGG - chr5_1:50507623-50507642 None:intergenic 20.0%
!! TGTAGTATAAGTTATCAAAA+TGG - chr5_1:50507628-50507647 None:intergenic 20.0%
!! AAGAATCTCATTAACAAAAA+GGG + chr5_1:50507647-50507666 Msa0735320:CDS 20.0%
!! TTTTGCTAAGTAGTAGTATA+AGG - chr5_1:50503446-50503465 None:intergenic 25.0%
! TATACTACTACTTAGCAAAA+TGG + chr5_1:50503446-50503465 Msa0735320:exon 25.0%
! TTTACAGCCTTAAAAACATT+GGG - chr5_1:50503585-50503604 None:intergenic 25.0%
! CATCATCATTAAATTAGTGA+CGG - chr5_1:50503896-50503915 None:intergenic 25.0%
!! TAACGAGAAAAATTTAGAGT+AGG + chr5_1:50504007-50504026 Msa0735320:intron 25.0%
! GAATATTGAGTGAATTGAAT+AGG + chr5_1:50504280-50504299 Msa0735320:intron 25.0%
!! CACTTTTAAATTCTTGAGAT+AGG + chr5_1:50504644-50504663 Msa0735320:intron 25.0%
!!! AACAACAGTACTTTTTTTAG+AGG + chr5_1:50504813-50504832 Msa0735320:intron 25.0%
!!! ACAACAGTACTTTTTTTAGA+GGG + chr5_1:50504814-50504833 Msa0735320:intron 25.0%
!! ATGTTTAAAGTAAAGAACGT+CGG - chr5_1:50504993-50505012 None:intergenic 25.0%
!! AGTTTTTGCTGATAATTTTC+TGG - chr5_1:50505687-50505706 None:intergenic 25.0%
! TACTAGAACAAAAGGAAAAT+TGG - chr5_1:50505947-50505966 None:intergenic 25.0%
! ATAATTTCGAAGAATCTGTT+TGG + chr5_1:50506039-50506058 Msa0735320:intron 25.0%
! AGTTATGTTTAGCAAAATCA+AGG - chr5_1:50506146-50506165 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr5_1:50506254-50506273 Msa0735320:intron 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG + chr5_1:50506342-50506361 Msa0735320:intron 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG + chr5_1:50506343-50506362 Msa0735320:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr5_1:50506364-50506383 Msa0735320:intron 25.0%
!! TTCTATTATATTTTGCAGCT+TGG + chr5_1:50506590-50506609 Msa0735320:intron 25.0%
!! ATTAAATGTGTTTTCTTGGT+TGG - chr5_1:50507045-50507064 None:intergenic 25.0%
!! TGTGTTATGTAAGTTGAATT+TGG - chr5_1:50507231-50507250 None:intergenic 25.0%
! CAAGAATCTCATTAACAAAA+AGG + chr5_1:50507646-50507665 Msa0735320:CDS 25.0%
!! TAGTATAAGGCTTTAGAAGA+TGG - chr5_1:50503433-50503452 None:intergenic 30.0%
GTTTACAGCCTTAAAAACAT+TGG - chr5_1:50503586-50503605 None:intergenic 30.0%
! TTGGTAGTTGATCATAGTAA+TGG - chr5_1:50503822-50503841 None:intergenic 30.0%
! ATTTCTCTAGCAAGTTTTCT+TGG - chr5_1:50503841-50503860 None:intergenic 30.0%
! GTAGGTAAATTTTGTGATGA+TGG + chr5_1:50504025-50504044 Msa0735320:intron 30.0%
! TACTTTTCCTATCTCTTTCA+AGG + chr5_1:50504358-50504377 Msa0735320:intron 30.0%
TGACGTAACTATTGCAATAA+TGG + chr5_1:50504393-50504412 Msa0735320:intron 30.0%
ACGACATCAAAAAATTACAG+GGG - chr5_1:50504688-50504707 None:intergenic 30.0%
CACGACATCAAAAAATTACA+GGG - chr5_1:50504689-50504708 None:intergenic 30.0%
TCACGACATCAAAAAATTAC+AGG - chr5_1:50504690-50504709 None:intergenic 30.0%
!!! CAACAGTACTTTTTTTAGAG+GGG + chr5_1:50504815-50504834 Msa0735320:intron 30.0%
! CCGATGTTTATGAAGTTTTA+GGG - chr5_1:50505174-50505193 None:intergenic 30.0%
CCCTAAAACTTCATAAACAT+CGG + chr5_1:50505171-50505190 Msa0735320:intron 30.0%
! TCCGATGTTTATGAAGTTTT+AGG - chr5_1:50505175-50505194 None:intergenic 30.0%
! ACTTCATAAACATCGGATTT+TGG + chr5_1:50505178-50505197 Msa0735320:intron 30.0%
TATTGATGCCATAAAGTCTA+AGG + chr5_1:50505333-50505352 Msa0735320:CDS 30.0%
!! CTGGTGTCTTTAAAACATAT+TGG - chr5_1:50505668-50505687 None:intergenic 30.0%
AAGTCACTTACTAGAACAAA+AGG - chr5_1:50505955-50505974 None:intergenic 30.0%
GAGTGAGAAGTTATAATTCT+TGG + chr5_1:50506096-50506115 Msa0735320:intron 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr5_1:50506239-50506258 Msa0735320:intron 30.0%
! TATTATATTTTGCAGCTTGG+AGG + chr5_1:50506593-50506612 Msa0735320:intron 30.0%
! ATTATATTTTGCAGCTTGGA+GGG + chr5_1:50506594-50506613 Msa0735320:intron 30.0%
!! GAGCTTTAAACCTATTGATA+AGG - chr5_1:50506721-50506740 None:intergenic 30.0%
! ATCAATAGGTTTAAAGCTCA+AGG + chr5_1:50506722-50506741 Msa0735320:CDS 30.0%
AAAAAGACATGTACCAGATA+AGG - chr5_1:50506771-50506790 None:intergenic 30.0%
TGCTTGATATATCACACATT+TGG + chr5_1:50506903-50506922 Msa0735320:intron 30.0%
! CATTTTCGCTTAATATCGAA+GGG - chr5_1:50507274-50507293 None:intergenic 30.0%
TTTAGATTATCAGACCATCA+AGG - chr5_1:50507299-50507318 None:intergenic 30.0%
! TGGTCTGATAATCTAAAGTT+CGG + chr5_1:50507302-50507321 Msa0735320:intron 30.0%
! TTGTTTTCTTTGTGCTTCTT+AGG + chr5_1:50507437-50507456 Msa0735320:intron 30.0%
GTAAATACGACCTCTATAAA+CGG - chr5_1:50507492-50507511 None:intergenic 30.0%
ATAGTTTATTTGCCAAGTCA+AGG + chr5_1:50507528-50507547 Msa0735320:intron 30.0%
!! TTTATTGTACCACTTTTCCT+TGG - chr5_1:50507566-50507585 None:intergenic 30.0%
!! ATAAGGCTTTAGAAGATGGA+TGG - chr5_1:50503429-50503448 None:intergenic 35.0%
!! GAAGTGTCCCAATGTTTTTA+AGG + chr5_1:50503575-50503594 Msa0735320:CDS 35.0%
AGTCATGAAAATGAAGACGA+AGG - chr5_1:50503659-50503678 None:intergenic 35.0%
ATTTCTCCCTCGATAAATTC+TGG + chr5_1:50504065-50504084 Msa0735320:intron 35.0%
ATTACGCCAGAATTTATCGA+GGG - chr5_1:50504074-50504093 None:intergenic 35.0%
AATTACGCCAGAATTTATCG+AGG - chr5_1:50504075-50504094 None:intergenic 35.0%
AGTAACCAAGTGTTCATATG+AGG + chr5_1:50504163-50504182 Msa0735320:intron 35.0%
GAAATCCTCATATGAACACT+TGG - chr5_1:50504171-50504190 None:intergenic 35.0%
! TGAGTTGAATATGATCAGTG+AGG + chr5_1:50504258-50504277 Msa0735320:intron 35.0%
TAAGACTCCTTGAAAGAGAT+AGG - chr5_1:50504368-50504387 None:intergenic 35.0%
TATTCTCACCAACCTATGTT+GGG - chr5_1:50504520-50504539 None:intergenic 35.0%
ATATTCTCACCAACCTATGT+TGG - chr5_1:50504521-50504540 None:intergenic 35.0%
!!! CTTGAGATAGGGTGTTTTTT+TGG + chr5_1:50504656-50504675 Msa0735320:intron 35.0%
ACGTACATCAAGCTTCAAAA+AGG - chr5_1:50504943-50504962 None:intergenic 35.0%
!!! TGAAGTTTTAGGGCAATGAT+AGG - chr5_1:50505164-50505183 None:intergenic 35.0%
! TTTTCTAAACAGGGTTGTGA+TGG + chr5_1:50505226-50505245 Msa0735320:intron 35.0%
GTCTTCTCTCCTTTATTAGA+GGG - chr5_1:50505274-50505293 None:intergenic 35.0%
AGTCTTCTCTCCTTTATTAG+AGG - chr5_1:50505275-50505294 None:intergenic 35.0%
CCTCTCAAAGAATCTTTGTT+GGG - chr5_1:50505304-50505323 None:intergenic 35.0%
ACCTCTCAAAGAATCTTTGT+TGG - chr5_1:50505305-50505324 None:intergenic 35.0%
ATGGGTCAAATAAATTGGGA+CGG - chr5_1:50506191-50506210 None:intergenic 35.0%
TCCCAATTTATTTGACCCAT+TGG + chr5_1:50506191-50506210 Msa0735320:intron 35.0%
GCCAATGGGTCAAATAAATT+GGG - chr5_1:50506195-50506214 None:intergenic 35.0%
AGCCAATGGGTCAAATAAAT+TGG - chr5_1:50506196-50506215 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr5_1:50506378-50506397 Msa0735320:intron 35.0%
CCAAGTTTAACCTTCCAAAA+TGG - chr5_1:50506620-50506639 None:intergenic 35.0%
!! CCATTTTGGAAGGTTAAACT+TGG + chr5_1:50506617-50506636 Msa0735320:CDS 35.0%
GAAGGTTAAACTTGGAAGAA+GGG + chr5_1:50506625-50506644 Msa0735320:CDS 35.0%
CCTATTGATAAGGTTGCTAA+GGG - chr5_1:50506711-50506730 None:intergenic 35.0%
ACCTATTGATAAGGTTGCTA+AGG - chr5_1:50506712-50506731 None:intergenic 35.0%
TATCACACATTTGGTTGACT+AGG + chr5_1:50506912-50506931 Msa0735320:intron 35.0%
! CCATTTTCGCTTAATATCGA+AGG - chr5_1:50507275-50507294 None:intergenic 35.0%
CCTTCGATATTAAGCGAAAA+TGG + chr5_1:50507272-50507291 Msa0735320:intron 35.0%
! CGCAAGTGATTAATGAGTTT+TGG - chr5_1:50507408-50507427 None:intergenic 35.0%
AAAGCTCTTGATCAGAATGA+AGG - chr5_1:50507676-50507695 None:intergenic 35.0%
! TCTGATCAAGAGCTTTTCAA+TGG + chr5_1:50507680-50507699 Msa0735320:CDS 35.0%
TGCTACTGCAATGATTAAGA+TGG + chr5_1:50507766-50507785 Msa0735320:CDS 35.0%
GCTACTGCAATGATTAAGAT+GGG + chr5_1:50507767-50507786 Msa0735320:CDS 35.0%
! TTTTCATGTCGTTACGCTAC+TGG + chr5_1:50504314-50504333 Msa0735320:intron 40.0%
AAATCGCTCTCAAAGATGAG+TGG - chr5_1:50504489-50504508 None:intergenic 40.0%
ATTTACCATGTCCCCAACAT+AGG + chr5_1:50504505-50504524 Msa0735320:intron 40.0%
ATTCTCACCAACCTATGTTG+GGG - chr5_1:50504519-50504538 None:intergenic 40.0%
AAATTTAGACCGTGTGCATG+TGG + chr5_1:50505036-50505055 Msa0735320:intron 40.0%
TTTGTAAAGCCACATGCACA+CGG - chr5_1:50505048-50505067 None:intergenic 40.0%
GATGACACACCCTCTAATAA+AGG + chr5_1:50505262-50505281 Msa0735320:CDS 40.0%
CCCAACAAAGATTCTTTGAG+AGG + chr5_1:50505301-50505320 Msa0735320:CDS 40.0%
TGATGCCATAAAGTCTAAGG+TGG + chr5_1:50505336-50505355 Msa0735320:CDS 40.0%
TGACTCCACCTTAGACTTTA+TGG - chr5_1:50505344-50505363 None:intergenic 40.0%
TCTACAGTCTCTCTACATCA+AGG - chr5_1:50505734-50505753 None:intergenic 40.0%
!! GAATCTGTTTGGTTTGACGA+TGG + chr5_1:50506050-50506069 Msa0735320:intron 40.0%
! TCTTGGCTTTCTTGTTGACA+TGG + chr5_1:50506113-50506132 Msa0735320:intron 40.0%
GTCAAATAAATTGGGACGGA+GGG - chr5_1:50506187-50506206 None:intergenic 40.0%
TCTGTGTGAAATGAGCCAAT+GGG - chr5_1:50506209-50506228 None:intergenic 40.0%
ATCTGTGTGAAATGAGCCAA+TGG - chr5_1:50506210-50506229 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr5_1:50506379-50506398 Msa0735320:intron 40.0%
GGAAGGTTAAACTTGGAAGA+AGG + chr5_1:50506624-50506643 Msa0735320:CDS 40.0%
AGGTTGCTAAGGGTAGAAAA+GGG - chr5_1:50506701-50506720 None:intergenic 40.0%
AAGGTTGCTAAGGGTAGAAA+AGG - chr5_1:50506702-50506721 None:intergenic 40.0%
CCCTTAGCAACCTTATCAAT+AGG + chr5_1:50506708-50506727 Msa0735320:CDS 40.0%
AGCATGACATGAAGTTTACG+TGG - chr5_1:50506955-50506974 None:intergenic 40.0%
TAAGCGAAAATGGACCTTGA+TGG + chr5_1:50507282-50507301 Msa0735320:intron 40.0%
TTCTTAGGAGCTCACACAAT+TGG + chr5_1:50507452-50507471 Msa0735320:intron 40.0%
CTCACACAATTGGAAAAGCA+AGG + chr5_1:50507462-50507481 Msa0735320:intron 40.0%
AGGAATTGTCCAAGGAAAAG+TGG + chr5_1:50507554-50507573 Msa0735320:CDS 40.0%
!!! GGTTTGGTGTTTTGAAGTCT+AGG - chr5_1:50507607-50507626 None:intergenic 40.0%
! GATCAAGAGCTTTTCAATGG+AGG + chr5_1:50507683-50507702 Msa0735320:CDS 40.0%
!! TGGAGGATCTACTGATTCAT+TGG + chr5_1:50507700-50507719 Msa0735320:CDS 40.0%
GGAAATAACAAGCCTCTTAC+CGG + chr5_1:50507788-50507807 Msa0735320:CDS 40.0%
TTGCTTCCTAATCTCACCTT+TGG - chr5_1:50507816-50507835 None:intergenic 40.0%
AACAGAATCACGAGCAGCAA+TGG - chr5_1:50505404-50505423 None:intergenic 45.0%
GGCATGATATGCAATGTACG+TGG - chr5_1:50505926-50505945 None:intergenic 45.0%
GGTCAAATAAATTGGGACGG+AGG - chr5_1:50506188-50506207 None:intergenic 45.0%
ACTGTTTGCATCATTGAGGC+TGG - chr5_1:50506663-50506682 None:intergenic 45.0%
AGCCTCAATGATGCAAACAG+TGG + chr5_1:50506662-50506681 Msa0735320:CDS 45.0%
CACCACTGTTTGCATCATTG+AGG - chr5_1:50506667-50506686 None:intergenic 45.0%
TTGCTAAGGGTAGAAAAGGG+AGG - chr5_1:50506698-50506717 None:intergenic 45.0%
AGCTCAAGGACTCTCTACTA+AGG + chr5_1:50506736-50506755 Msa0735320:CDS 45.0%
AGGACTCTCTACTAAGGACA+TGG + chr5_1:50506742-50506761 Msa0735320:CDS 45.0%
ACTCTCTACTAAGGACATGG+TGG + chr5_1:50506745-50506764 Msa0735320:CDS 45.0%
!! GTACTCTGACTCTAGAGTCT+TGG + chr5_1:50507141-50507160 Msa0735320:intron 45.0%
GCAAGGTGTACCGTTTATAG+AGG + chr5_1:50507479-50507498 Msa0735320:intron 45.0%
TATTTGCCAAGTCAAGGCAG+AGG + chr5_1:50507534-50507553 Msa0735320:intron 45.0%
ACAATTCCTCTGCCTTGACT+TGG - chr5_1:50507543-50507562 None:intergenic 45.0%
CCTAATCTCACCTTTGGATC+CGG - chr5_1:50507810-50507829 None:intergenic 45.0%
GTGAGATTAGGAAGCAATGC+AGG + chr5_1:50507819-50507838 Msa0735320:CDS 45.0%
AGCGACAGCAGAGTGAACAA+CGG - chr5_1:50503610-50503629 None:intergenic 50.0%
ACCATGTCCCCAACATAGGT+TGG + chr5_1:50504509-50504528 Msa0735320:intron 50.0%
ACCAACCTATGTTGGGGACA+TGG - chr5_1:50504513-50504532 None:intergenic 50.0%
ATCAGCACATGAGACCACAC+CGG - chr5_1:50505374-50505393 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr5_1:50506383-50506402 Msa0735320:intron 50.0%
CTAAGGGTAGAAAAGGGAGG+TGG - chr5_1:50506695-50506714 None:intergenic 50.0%
!! AAGGACATGGTGGCCTTATC+TGG + chr5_1:50506755-50506774 Msa0735320:CDS 50.0%
CAAGGCAGAGGAATTGTCCA+AGG + chr5_1:50507546-50507565 Msa0735320:intron 50.0%
AAGCCTCTTACCGGATCCAA+AGG + chr5_1:50507797-50507816 Msa0735320:CDS 50.0%
TCACCTTTGGATCCGGTAAG+AGG - chr5_1:50507803-50507822 None:intergenic 50.0%
CCGGATCCAAAGGTGAGATT+AGG + chr5_1:50507807-50507826 Msa0735320:CDS 50.0%
TGTCGCTAGAGAACCTCGCA+TGG + chr5_1:50503623-50503642 Msa0735320:CDS 55.0%
GTCGCTAGAGAACCTCGCAT+GGG + chr5_1:50503624-50503643 Msa0735320:CDS 55.0%
AAGGTGGAGTCAGTATGCCC+CGG + chr5_1:50505352-50505371 Msa0735320:CDS 55.0%
TCAGCACATGAGACCACACC+GGG - chr5_1:50505373-50505392 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr5_1:50506387-50506406 Msa0735320:intron 55.0%
! TGCAGCTTGGAGGGCCATTT+TGG + chr5_1:50506603-50506622 Msa0735320:intron 55.0%
!! GCTTGGAGGGCCATTTTGGA+AGG + chr5_1:50506607-50506626 Msa0735320:intron 55.0%
GCTAGAGAACCTCGCATGGG+AGG + chr5_1:50503627-50503646 Msa0735320:CDS 60.0%
AGGAGAGATCCTCCCATGCG+AGG - chr5_1:50503639-50503658 None:intergenic 60.0%
CAGCACATGAGACCACACCG+GGG - chr5_1:50505372-50505391 None:intergenic 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr5_1:50506386-50506405 Msa0735320:intron 60.0%
AGCTTGTACTCCCTCCGTCC+CGG - chr5_1:50506400-50506419 None:intergenic 60.0%
GGAGTCAGTATGCCCCGGTG+TGG + chr5_1:50505357-50505376 Msa0735320:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 50503355 50507853 50503355 ID=Msa0735320;Name=Msa0735320
chr5_1 mRNA 50503355 50507853 50503355 ID=Msa0735320-mRNA-1;Parent=Msa0735320;Name=Msa0735320-mRNA-1;_AED=0.34;_eAED=0.34;_QI=110|0.66|0.5|1|1|1|4|0|301
chr5_1 exon 50503355 50503689 50503355 ID=Msa0735320-mRNA-1:exon:14246;Parent=Msa0735320-mRNA-1
chr5_1 exon 50505238 50505429 50505238 ID=Msa0735320-mRNA-1:exon:14247;Parent=Msa0735320-mRNA-1
chr5_1 exon 50506608 50506796 50506608 ID=Msa0735320-mRNA-1:exon:14248;Parent=Msa0735320-mRNA-1
chr5_1 exon 50507554 50507853 50507554 ID=Msa0735320-mRNA-1:exon:14249;Parent=Msa0735320-mRNA-1
chr5_1 five_prime_UTR 50503355 50503464 50503355 ID=Msa0735320-mRNA-1:five_prime_utr;Parent=Msa0735320-mRNA-1
chr5_1 CDS 50503465 50503689 50503465 ID=Msa0735320-mRNA-1:cds;Parent=Msa0735320-mRNA-1
chr5_1 CDS 50505238 50505429 50505238 ID=Msa0735320-mRNA-1:cds;Parent=Msa0735320-mRNA-1
chr5_1 CDS 50506608 50506796 50506608 ID=Msa0735320-mRNA-1:cds;Parent=Msa0735320-mRNA-1
chr5_1 CDS 50507554 50507853 50507554 ID=Msa0735320-mRNA-1:cds;Parent=Msa0735320-mRNA-1
Gene Sequence

>Msa0735320

ATGGCACTTTCTTCTTCTAAAGTTTCACTAAACTTTTTTTGCTTTGTTTTGTTCATGTTTTATCTTATAGATGGTTCTTTTTCTCAACTATCTGAAAATTTCTATGCTAAGAAGTGTCCCAATGTTTTTAAGGCTGTAAACTCCGTTGTTCACTCTGCTGTCGCTAGAGAACCTCGCATGGGAGGATCTCTCCTTCGTCTTCATTTTCATGACTGCTTTGTCAATGGTTGTGATGGATCAGTGCTACTTGATGACACACCCTCTAATAAAGGAGAGAAGACTGCACTTCCCAACAAAGATTCTTTGAGAGGTTTTGAAGTTATTGATGCCATAAAGTCTAAGGTGGAGTCAGTATGCCCCGGTGTGGTCTCATGTGCTGATATCTTAGCCATTGCTGCTCGTGATTCTGTTGTAAATCTTGGAGGGCCATTTTGGAAGGTTAAACTTGGAAGAAGGGATTCAAAAACAGCCAGCCTCAATGATGCAAACAGTGGTGTAATTCCACCTCCCTTTTCTACCCTTAGCAACCTTATCAATAGGTTTAAAGCTCAAGGACTCTCTACTAAGGACATGGTGGCCTTATCTGGTACATGTCTTTTTCCTATTAGGAATTGTCCAAGGAAAAGTGGTACAATAAAAGACAACAATGTAGCTGTCCTAGACTTCAAAACACCAAACCATTTTGATAACTTATACTACAAGAATCTCATTAACAAAAAGGGACTCCTTCATTCTGATCAAGAGCTTTTCAATGGAGGATCTACTGATTCATTGGTTAAATCTTATAGCAATAATCAAAATGCATTTGAATCTGATTTTGCTACTGCAATGATTAAGATGGGAAATAACAAGCCTCTTACCGGATCCAAAGGTGAGATTAGGAAGCAATGCAGGAGAGCCAATTAA
Protein sequence

>Msa0735320

MALSSSKVSLNFFCFVLFMFYLIDGSFSQLSENFYAKKCPNVFKAVNSVVHSAVAREPRMGGSLLRLHFHDCFVNGCDGSVLLDDTPSNKGEKTALPNKDSLRGFEVIDAIKSKVESVCPGVVSCADILAIAARDSVVNLGGPFWKVKLGRRDSKTASLNDANSGVIPPPFSTLSNLINRFKAQGLSTKDMVALSGTCLFPIRNCPRKSGTIKDNNVAVLDFKTPNHFDNLYYKNLINKKGLLHSDQELFNGGSTDSLVKSYSNNQNAFESDFATAMIKMGNNKPLTGSKGEIRKQCRRAN*