AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0854850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0854850 MtrunA17_Chr5g0432441 90.184 326 15 1 1 309 1 326 0.0 588
Msa0854850 MtrunA17_Chr3g0106921 65.723 318 91 2 9 309 4 320 5.65e-148 418
Msa0854850 MtrunA17_Chr3g0129051 59.692 325 107 6 3 309 4 322 2.08e-128 369
Msa0854850 MtrunA17_Chr5g0438021 58.095 315 113 4 12 309 13 325 1.18e-122 354
Msa0854850 MtrunA17_Chr4g0013171 57.585 323 116 4 5 309 2 321 2.88e-117 340
Msa0854850 MtrunA17_Chr3g0129061 58.842 311 108 5 17 309 14 322 4.13e-114 332
Msa0854850 MtrunA17_Chr3g0129041 58.521 311 109 4 17 309 14 322 9.78e-112 326
Msa0854850 MtrunA17_Chr4g0013201 54.333 300 116 3 27 309 43 338 1.91e-110 323
Msa0854850 MtrunA17_Chr4g0013191 54.545 308 116 5 22 309 37 340 9.30e-110 322
Msa0854850 MtrunA17_Chr4g0013181 58.644 295 101 3 33 309 62 353 1.13e-109 322
Msa0854850 MtrunA17_Chr4g0038161 52.229 314 129 3 15 309 9 320 1.00e-108 318
Msa0854850 MtrunA17_Chr3g0114451 54.517 321 123 5 8 309 2 318 3.15e-108 317
Msa0854850 MtrunA17_Chr7g0244461 55.518 299 112 4 28 309 18 312 1.49e-107 315
Msa0854850 MtrunA17_Chr4g0038151 52.050 317 126 5 15 309 9 321 6.26e-107 314
Msa0854850 MtrunA17_Chr4g0043391 52.063 315 129 4 15 309 12 324 2.08e-106 313
Msa0854850 MtrunA17_Chr4g0038171 52.063 315 129 4 15 309 9 321 4.41e-106 312
Msa0854850 MtrunA17_Chr2g0320071 53.185 314 124 5 15 309 8 317 3.38e-105 310
Msa0854850 MtrunA17_Chr7g0244451 55.161 310 118 4 17 309 11 316 1.75e-104 308
Msa0854850 MtrunA17_Chr5g0432341 52.484 322 126 9 10 309 5 321 1.48e-102 303
Msa0854850 MtrunA17_Chr2g0320101 53.512 299 118 4 28 309 23 317 3.61e-102 302
Msa0854850 MtrunA17_Chr5g0432351 52.174 322 127 9 10 309 5 321 5.29e-102 301
Msa0854850 MtrunA17_Chr3g0106901 54.462 325 121 7 7 309 4 323 5.53e-102 301
Msa0854850 MtrunA17_Chr1g0213061 49.240 329 139 6 1 309 1 321 1.69e-100 298
Msa0854850 MtrunA17_Chr5g0432331 51.227 326 130 10 6 309 34 352 9.63e-100 297
Msa0854850 MtrunA17_Chr2g0320091 51.757 313 130 6 15 309 9 318 4.20e-99 294
Msa0854850 MtrunA17_Chr2g0320061 51.250 320 133 6 9 309 3 318 8.58e-98 291
Msa0854850 MtrunA17_Chr3g0107881 49.375 320 136 6 12 309 9 324 3.56e-97 289
Msa0854850 MtrunA17_Chr7g0224371 50.301 332 132 9 1 309 1 322 9.44e-96 286
Msa0854850 MtrunA17_Chr2g0320051 52.000 300 122 5 28 309 23 318 2.78e-95 284
Msa0854850 MtrunA17_Chr2g0320031 49.684 316 137 5 12 309 8 319 1.13e-94 283
Msa0854850 MtrunA17_Chr5g0432401 51.282 312 119 8 1 307 1 284 2.34e-93 278
Msa0854850 MtrunA17_Chr5g0402571 47.975 321 139 4 13 309 10 326 6.65e-93 278
Msa0854850 MtrunA17_Chr5g0402581 47.664 321 140 4 13 309 10 326 1.39e-92 278
Msa0854850 MtrunA17_Chr1g0175561 47.205 322 139 6 15 309 9 326 4.32e-91 274
Msa0854850 MtrunA17_Chr7g0258811 45.706 326 144 7 12 309 51 371 2.61e-89 271
Msa0854850 MtrunA17_Chr5g0404721 48.852 305 127 4 30 309 30 330 6.45e-89 268
Msa0854850 MtrunA17_Chr4g0070951 48.160 326 134 7 13 309 10 329 1.59e-86 262
Msa0854850 MtrunA17_Chr1g0155611 48.693 306 127 4 30 309 46 347 9.79e-86 261
Msa0854850 MtrunA17_Chr2g0292101 45.141 319 145 5 17 309 12 326 1.47e-85 260
Msa0854850 MtrunA17_Chr8g0372601 47.883 307 131 4 28 309 28 330 3.65e-85 259
Msa0854850 MtrunA17_Chr2g0292091 44.343 327 152 5 9 309 3 325 9.71e-85 258
Msa0854850 MtrunA17_Chr4g0068621 48.525 305 130 5 28 309 36 336 3.70e-84 256
Msa0854850 MtrunA17_Chr2g0299691 47.561 328 134 8 14 309 37 358 7.62e-84 256
Msa0854850 MtrunA17_Chr2g0292151 45.783 332 150 6 4 309 3 330 5.81e-82 251
Msa0854850 MtrunA17_Chr2g0291751 44.611 334 154 6 3 309 1 330 5.00e-81 249
Msa0854850 MtrunA17_Chr2g0292251 46.926 309 132 6 28 309 26 329 6.49e-81 249
Msa0854850 MtrunA17_Chr2g0292241 44.681 329 150 6 8 309 7 330 2.04e-80 248
Msa0854850 MtrunA17_Chr2g0292211 44.615 325 147 7 13 309 22 341 3.40e-80 247
Msa0854850 MtrunA17_Chr2g0292181 45.031 322 147 6 14 309 22 339 4.87e-80 247
Msa0854850 MtrunA17_Chr5g0400331 46.774 310 131 5 30 309 26 331 2.70e-79 244
Msa0854850 MtrunA17_Chr2g0292111 44.611 334 154 7 3 309 1 330 5.67e-79 244
Msa0854850 MtrunA17_Chr2g0292271 43.807 331 151 7 8 309 6 330 2.28e-77 240
Msa0854850 MtrunA17_Chr5g0421411 45.017 291 123 6 50 309 1 285 5.14e-77 236
Msa0854850 MtrunA17_Chr4g0013721 44.231 312 141 7 23 309 93 396 6.79e-77 240
Msa0854850 MtrunA17_Chr4g0013821 42.581 310 139 8 28 309 26 324 3.33e-74 231
Msa0854850 MtrunA17_Chr2g0292291 42.236 322 157 6 14 309 14 332 1.34e-73 230
Msa0854850 MtrunA17_Chr2g0292261 42.284 324 153 6 14 309 13 330 9.62e-73 228
Msa0854850 MtrunA17_Chr6g0477071 40.312 320 164 7 8 309 32 342 5.18e-71 223
Msa0854850 MtrunA17_Chr1g0188431 38.393 336 171 6 1 309 1 327 6.19e-71 222
Msa0854850 MtrunA17_Chr2g0322861 40.625 320 158 7 16 309 73 386 3.29e-69 220
Msa0854850 MtrunA17_Chr4g0024121 37.342 316 174 5 12 305 9 322 2.25e-67 213
Msa0854850 MtrunA17_Chr1g0194151 39.286 336 174 7 1 309 1 333 4.33e-67 213
Msa0854850 MtrunA17_Chr6g0460711 38.462 312 170 4 16 309 7 314 1.32e-65 208
Msa0854850 MtrunA17_Chr1g0164891 40.924 303 149 5 34 309 93 392 1.73e-65 210
Msa0854850 MtrunA17_Chr7g0225221 40.061 327 163 8 7 309 3 320 3.70e-65 207
Msa0854850 MtrunA17_Chr3g0128061 38.889 324 171 4 10 309 6 326 7.92e-65 207
Msa0854850 MtrunA17_Chr1g0204011 37.654 324 168 7 11 309 8 322 3.11e-64 205
Msa0854850 MtrunA17_Chr2g0279041 39.077 325 159 9 15 309 10 325 1.45e-63 203
Msa0854850 MtrunA17_Chr1g0194271 38.209 335 172 6 8 309 8 340 2.62e-63 203
Msa0854850 MtrunA17_Chr4g0046131 40.974 349 155 10 1 305 1 342 2.93e-63 203
Msa0854850 MtrunA17_Chr1g0157051 39.432 317 157 7 15 305 7 314 3.88e-63 202
Msa0854850 MtrunA17_Chr6g0467161 40.061 327 158 8 12 309 19 336 1.53e-62 201
Msa0854850 MtrunA17_Chr5g0405991 38.801 317 160 7 17 309 10 316 1.34e-61 198
Msa0854850 MtrunA17_Chr7g0254201 37.248 298 166 4 26 306 16 309 1.02e-60 196
Msa0854850 MtrunA17_Chr4g0063671 37.423 326 163 9 13 309 7 320 9.12e-60 193
Msa0854850 MtrunA17_Chr4g0076211 37.333 300 151 7 35 309 46 333 2.36e-59 193
Msa0854850 MtrunA17_Chr2g0296621 37.377 305 153 8 30 309 50 341 9.24e-58 189
Msa0854850 MtrunA17_Chr8g0371381 37.864 309 148 8 30 309 26 319 1.23e-57 188
Msa0854850 MtrunA17_Chr7g0268501 39.241 316 165 10 15 309 11 320 2.21e-57 187
Msa0854850 MtrunA17_Chr7g0268521 39.241 316 165 10 15 309 11 320 2.21e-57 187
Msa0854850 MtrunA17_Chr7g0254231 35.739 291 169 4 30 306 22 308 4.54e-56 184
Msa0854850 MtrunA17_Chr6g0451571 37.500 320 171 8 17 309 17 334 4.45e-55 182
Msa0854850 MtrunA17_Chr6g0467251 34.940 332 181 8 1 305 1 324 1.27e-54 181
Msa0854850 MtrunA17_Chr8g0364291 36.875 320 178 6 13 309 6 324 2.03e-54 180
Msa0854850 MtrunA17_Chr2g0278671 38.050 318 155 9 23 309 17 323 2.36e-54 179
Msa0854850 MtrunA17_Chr2g0316791 36.228 334 156 10 28 307 74 404 4.12e-54 181
Msa0854850 MtrunA17_Chr1g0197411 35.258 329 182 9 11 309 3 330 1.06e-53 178
Msa0854850 MtrunA17_Chr2g0316481 36.826 334 154 11 28 307 74 404 1.19e-53 180
Msa0854850 MtrunA17_Chr2g0316191 36.119 335 156 10 28 307 74 405 1.84e-53 180
Msa0854850 MtrunA17_Chr4g0053871 39.672 305 152 10 26 305 23 320 6.73e-53 176
Msa0854850 MtrunA17_Chr2g0316831 36.228 334 156 10 28 307 110 440 1.58e-52 178
Msa0854850 MtrunA17_Chr4g0074851 34.268 321 188 6 10 309 10 328 2.36e-51 172
Msa0854850 MtrunA17_Chr5g0401431 35.220 318 171 7 17 305 14 325 6.96e-51 171
Msa0854850 MtrunA17_Chr4g0072091 38.264 311 152 10 29 309 23 323 1.72e-50 169
Msa0854850 MtrunA17_Chr3g0114461 49.479 192 75 4 136 309 5 192 2.69e-50 165
Msa0854850 MtrunA17_Chr2g0279061 40.769 260 117 7 74 305 12 262 4.33e-50 167
Msa0854850 MtrunA17_Chr6g0467121 36.333 300 161 7 30 309 74 363 1.56e-49 169
Msa0854850 MtrunA17_Chr1g0181431 34.106 302 178 5 27 309 24 323 2.53e-49 167
Msa0854850 MtrunA17_Chr5g0413051 33.226 310 174 5 29 309 72 377 3.17e-49 168
Msa0854850 MtrunA17_Chr1g0181481 35.216 301 174 5 28 309 25 323 3.63e-49 166
Msa0854850 MtrunA17_Chr2g0311251 34.328 335 162 11 28 308 9 339 7.39e-49 166
Msa0854850 MtrunA17_Chr1g0148021 33.233 331 186 6 9 309 3 328 8.62e-49 165
Msa0854850 MtrunA17_Chr4g0075241 33.224 304 176 5 29 309 28 327 1.17e-48 165
Msa0854850 MtrunA17_Chr1g0181511 34.551 301 176 5 28 309 25 323 4.25e-48 163
Msa0854850 MtrunA17_Chr3g0136751 31.902 326 194 5 7 309 2 322 3.24e-47 161
Msa0854850 MtrunA17_Chr2g0311241 31.649 376 158 11 28 308 58 429 1.29e-46 162
Msa0854850 MtrunA17_Chr2g0329641 33.225 307 173 6 29 309 35 335 6.10e-46 158
Msa0854850 MtrunA17_Chr3g0114491 61.607 112 43 0 29 140 17 128 6.04e-43 144
Msa0854850 MtrunA17_Chr2g0316811 44.571 175 93 3 28 200 48 220 5.16e-42 148
Msa0854850 MtrunA17_Chr2g0316141 36.059 269 132 10 76 307 11 276 2.77e-40 142
Msa0854850 MtrunA17_Chr2g0316181 36.059 269 132 10 76 307 11 276 2.77e-40 142
Msa0854850 MtrunA17_Chr4g0004591 28.438 320 147 6 30 308 77 355 4.92e-35 130
Msa0854850 MtrunA17_Chr2g0292281 41.176 204 100 6 122 309 17 216 9.80e-32 118
Msa0854850 MtrunA17_Chr5g0432361 53.191 94 41 2 217 308 16 108 2.96e-26 100
Msa0854850 MtrunA17_Chr1g0181501 32.105 190 110 3 138 309 1 189 1.41e-23 95.5
Msa0854850 MtrunA17_Chr6g0468731 64.286 70 21 2 69 138 22 87 2.00e-22 89.4
Msa0854850 MtrunA17_Chr1g0181491 37.273 110 67 2 28 137 10 117 9.21e-17 75.9
Msa0854850 MtrunA17_Chr5g0432321 42.975 121 47 5 151 254 17 132 1.90e-16 74.7
Msa0854850 MtrunA17_Chr7g0254181 41.237 97 53 2 100 196 29 121 2.05e-16 75.1
Msa0854850 MtrunA17_Chr1g0196191 54.286 70 31 1 68 137 23 91 3.75e-16 74.3
Msa0854850 MtrunA17_Chr3g0104101 40.385 104 50 4 213 309 51 149 9.75e-16 73.2
Msa0854850 MtrunA17_Chr5g0423911 40.299 67 40 0 239 305 1 67 3.07e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0854850 AT5G58390.1 60.897 312 104 2 15 309 6 316 2.68e-122 353
Msa0854850 AT5G05340.1 60.535 299 99 3 28 309 28 324 4.88e-122 353
Msa0854850 AT5G58400.1 57.643 314 115 2 13 309 13 325 7.90e-122 352
Msa0854850 AT1G14550.1 52.366 317 126 6 15 309 8 321 8.27e-103 304
Msa0854850 AT1G14540.1 48.589 319 141 6 10 309 1 315 9.34e-93 278
Msa0854850 AT5G06730.1 44.543 339 153 7 1 309 1 334 4.28e-91 275
Msa0854850 AT4G36430.1 48.765 324 132 9 13 309 14 330 6.07e-91 274
Msa0854850 AT5G06720.1 45.846 325 142 6 15 309 13 333 2.92e-90 272
Msa0854850 AT3G50990.1 48.867 309 129 7 26 309 37 341 8.02e-89 269
Msa0854850 AT5G66390.1 48.553 311 129 7 25 309 28 333 1.46e-88 268
Msa0854850 AT2G18150.1 49.669 302 121 7 34 309 40 336 1.22e-87 266
Msa0854850 AT1G44970.1 47.771 314 133 5 23 309 37 346 1.39e-87 266
Msa0854850 AT4G16270.1 48.837 301 123 7 34 309 54 348 7.45e-84 256
Msa0854850 AT1G49570.1 47.883 307 131 4 25 305 43 346 1.57e-83 256
Msa0854850 AT2G18140.1 47.525 303 128 6 33 309 38 335 1.03e-82 253
Msa0854850 AT4G08770.1 46.349 315 131 8 26 309 20 327 5.16e-81 249
Msa0854850 AT5G19880.1 44.720 322 143 6 17 309 14 329 1.23e-80 248
Msa0854850 AT5G19890.1 46.273 322 135 7 16 309 16 327 8.23e-80 246
Msa0854850 AT2G38390.1 45.192 312 134 8 28 309 29 333 1.33e-79 246
Msa0854850 AT4G08780.1 45.397 315 134 8 26 309 20 327 2.66e-79 245
Msa0854850 AT1G68850.1 40.419 334 157 7 16 309 4 335 3.24e-79 244
Msa0854850 AT2G35380.1 45.367 313 142 5 25 309 24 335 9.20e-78 241
Msa0854850 AT2G38380.1 45.192 312 134 8 28 309 29 333 9.91e-78 241
Msa0854850 AT3G32980.1 43.631 314 141 7 26 309 27 334 1.78e-77 240
Msa0854850 AT3G49110.1 44.410 322 139 8 21 309 22 336 2.88e-77 240
Msa0854850 AT3G49120.1 43.631 314 141 7 26 309 28 335 4.91e-76 237
Msa0854850 AT2G22420.1 42.295 305 143 6 30 309 23 319 1.10e-70 222
Msa0854850 AT3G01190.1 40.000 320 155 9 17 309 12 321 1.34e-68 216
Msa0854850 AT1G71695.1 41.196 301 151 4 30 309 44 339 8.29e-68 216
Msa0854850 AT3G03670.1 38.365 318 173 5 13 309 6 321 9.44e-68 214
Msa0854850 AT4G33420.1 39.748 317 172 5 8 309 13 325 1.47e-67 214
Msa0854850 AT4G33420.2 39.748 317 172 5 8 309 60 372 8.74e-67 213
Msa0854850 AT1G05260.1 39.375 320 160 7 17 309 13 325 3.03e-66 211
Msa0854850 AT5G51890.1 37.383 321 170 6 13 309 9 322 3.24e-66 211
Msa0854850 AT4G11290.1 37.346 324 175 5 13 309 4 326 6.80e-66 210
Msa0854850 AT5G17820.1 38.750 320 160 8 13 309 7 313 3.49e-64 205
Msa0854850 AT5G17820.2 38.750 320 160 8 13 309 60 366 3.28e-63 204
Msa0854850 AT4G26010.2 37.778 315 179 5 5 306 24 334 1.26e-62 202
Msa0854850 AT4G26010.1 38.487 304 170 5 16 306 7 306 2.01e-62 200
Msa0854850 AT2G41480.1 37.015 335 169 11 8 309 3 328 5.26e-61 197
Msa0854850 AT5G40150.1 37.134 307 160 6 28 309 30 328 5.43e-61 197
Msa0854850 AT5G15180.1 35.000 340 177 8 3 309 1 329 8.14e-61 197
Msa0854850 AT5G42180.1 37.580 314 169 6 15 307 8 315 9.05e-61 196
Msa0854850 AT3G28200.1 36.964 303 165 6 28 309 19 316 1.40e-60 196
Msa0854850 AT5G14130.1 37.421 318 168 6 17 309 19 330 1.72e-60 196
Msa0854850 AT1G05240.1 38.796 299 157 7 30 305 26 321 1.76e-60 196
Msa0854850 AT1G05250.1 38.796 299 157 7 30 305 26 321 1.76e-60 196
Msa0854850 AT5G14130.2 37.580 314 165 6 17 305 60 367 5.44e-60 196
Msa0854850 AT5G64120.1 36.570 309 162 7 25 309 30 328 7.24e-60 194
Msa0854850 AT3G21770.1 37.419 310 168 6 22 308 21 327 1.02e-59 194
Msa0854850 AT1G77100.1 36.503 326 175 9 8 309 2 319 1.92e-59 193
Msa0854850 AT4G17690.1 39.465 299 160 5 30 309 26 322 1.56e-58 191
Msa0854850 AT5G47000.1 38.312 308 168 6 22 309 25 330 6.89e-58 189
Msa0854850 AT5G39580.1 37.654 324 167 8 11 309 6 319 9.45e-57 186
Msa0854850 AT2G24800.1 37.299 311 156 7 28 309 29 329 4.53e-56 184
Msa0854850 AT2G35380.2 44.898 245 107 4 92 309 4 247 1.81e-55 181
Msa0854850 AT1G30870.1 38.436 307 150 7 30 309 51 345 1.89e-55 184
Msa0854850 AT1G34510.1 36.928 306 176 5 17 309 8 309 4.18e-55 181
Msa0854850 AT5G67400.1 37.621 311 159 8 24 305 21 325 6.75e-55 181
Msa0854850 AT4G33870.1 32.665 349 175 7 18 308 54 400 1.87e-54 182
Msa0854850 AT4G31760.1 34.591 318 179 5 15 309 15 326 1.42e-53 178
Msa0854850 AT4G37530.1 39.048 315 151 11 23 305 20 325 1.52e-53 178
Msa0854850 AT2G18980.1 36.280 328 170 9 11 309 6 323 5.68e-53 176
Msa0854850 AT3G49960.1 36.335 322 168 9 13 305 12 325 6.44e-53 176
Msa0854850 AT1G24110.1 35.826 321 178 8 15 309 4 322 7.29e-53 176
Msa0854850 AT4G25980.1 35.514 321 166 10 19 309 17 326 1.68e-52 175
Msa0854850 AT4G37520.1 38.762 307 153 9 28 305 25 325 2.20e-52 175
Msa0854850 AT5G64110.1 36.858 331 164 9 14 309 10 330 1.16e-51 173
Msa0854850 AT5G64100.1 35.855 304 156 6 33 309 40 331 3.57e-51 172
Msa0854850 AT4G37520.2 37.582 306 155 8 28 305 25 322 6.35e-51 171
Msa0854850 AT4G30170.1 37.459 307 161 8 28 309 25 325 6.61e-50 168
Msa0854850 AT4G21960.1 35.452 299 172 3 30 309 31 327 2.78e-49 167
Msa0854850 AT2G34060.1 36.482 307 162 7 29 306 41 343 1.39e-48 166
Msa0854850 AT2G37130.1 33.333 306 172 9 29 309 29 327 2.81e-45 156
Msa0854850 AT2G37130.2 33.444 302 169 9 33 309 2 296 6.42e-45 155
Msa0854850 AT5G22410.1 34.328 335 178 11 1 306 1 322 7.47e-45 155
Msa0854850 AT2G39040.1 35.275 309 169 7 29 309 45 350 9.43e-45 155
Msa0854850 AT2G24800.2 45.604 182 92 3 28 209 29 203 2.01e-44 152
Msa0854850 AT4G37530.2 39.552 268 121 11 23 258 20 278 1.96e-43 150
Msa0854850 AT4G25980.2 41.436 181 101 3 26 206 67 242 9.54e-42 146
Msa0854850 AT5G24070.1 35.531 273 141 7 64 309 71 335 1.82e-41 147
Msa0854850 AT2G43480.1 31.731 312 178 6 26 309 31 335 2.95e-40 144
Msa0854850 AT5G39580.2 43.011 186 100 3 11 196 6 185 6.56e-40 140
Msa0854850 AT3G17070.1 29.139 302 192 2 30 309 38 339 5.50e-39 140
Msa0854850 AT3G17070.2 25.912 274 180 3 58 309 4 276 5.71e-26 104
Msa0854850 AT4G32320.1 30.081 246 114 7 64 291 117 322 1.01e-17 82.8
Msa0854850 AT3G42570.1 30.252 119 81 1 83 199 32 150 2.88e-12 63.9

Find 70 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTCCACCTTAGACTTTA+TGG 0.119850 5_4:-47739145 None:intergenic
TTATCCAGGTTTCCTTTAAT+TGG 0.201465 5_4:-47742107 None:intergenic
TGCATTCTTCTCTCCTTTAT+TGG 0.219837 5_4:-47739079 None:intergenic
CAAGAATCTCATCAACAAAA+AGG 0.247712 5_4:+47741907 Msa0854850:CDS
TTGTCTAAGTCTTTAGGATT+AGG 0.293094 5_4:+47737152 None:intergenic
CCAAGTTTAACCTTCCAAAA+AGG 0.308795 5_4:-47740899 None:intergenic
TGTAGTATAAGTTATCAAAA+TGG 0.316765 5_4:-47741886 None:intergenic
AAGGACATGGTGGCCTTATC+TGG 0.324620 5_4:+47741037 Msa0854850:CDS
GGAAATAACAAGCCTCTTAC+CGG 0.340680 5_4:+47742049 Msa0854850:CDS
CCTTATACTACTTAGCAAAA+TGG 0.343968 5_4:+47737252 Msa0854850:exon
AATGCAGGAGAGCCAATTAA+AGG 0.372028 5_4:+47742095 Msa0854850:CDS
CCCTTAGCAACCTTATCAAT+AGG 0.384569 5_4:+47740990 Msa0854850:CDS
CATTATATATAGAGATAATA+TGG 0.387093 5_4:-47737190 None:intergenic
AAGGTTGCTAAGGGTAGAAA+AGG 0.387632 5_4:-47740981 None:intergenic
GATCAAAAGGGGAGATTAGA+AGG 0.387743 5_4:+47742071 Msa0854850:CDS
TGCTACTGCAATGATTAAGA+TGG 0.388563 5_4:+47742027 Msa0854850:CDS
TGGAGGATCTACTGATTCAT+TGG 0.391305 5_4:+47741961 Msa0854850:CDS
GAGCTTTAAACCTATTGATA+AGG 0.399870 5_4:-47741000 None:intergenic
GATGACACACCCTCCAATAA+AGG 0.400602 5_4:+47739066 Msa0854850:CDS
TATTGATGCCATAAAGTCTA+AGG 0.403057 5_4:+47739137 Msa0854850:CDS
ACCTATTGATAAGGTTGCTA+AGG 0.417064 5_4:-47740991 None:intergenic
AAGCCTCTTACCGGATCAAA+AGG 0.417845 5_4:+47742058 Msa0854850:CDS
TTCTTCTCTCCTTTATTGGA+GGG 0.419465 5_4:-47739075 None:intergenic
ACCTCTCAAAGAATCTTTGT+TGG 0.427591 5_4:-47739106 None:intergenic
CCTCTCAAAGAATCTTTGTT+GGG 0.433102 5_4:-47739105 None:intergenic
GGAAGGTTAAACTTGGAAGA+AGG 0.441433 5_4:+47740906 Msa0854850:CDS
ATTCTTCTCTCCTTTATTGG+AGG 0.454303 5_4:-47739076 None:intergenic
GAAGGTTAAACTTGGAAGAA+GGG 0.455829 5_4:+47740907 Msa0854850:CDS
AAGTAGTATAAGGCTTTAGA+AGG 0.458660 5_4:-47737242 None:intergenic
TATAAGTTATCAAAATGGTT+TGG 0.466239 5_4:-47741881 None:intergenic
ATCAATAGGTTTAAAGCTCA+AGG 0.476560 5_4:+47741004 Msa0854850:CDS
ACAATTCCTCTGCCTTGACT+TGG 0.477817 5_4:-47741801 None:intergenic
ACTGTTTGCATCATTGAGGC+TGG 0.487052 5_4:-47740942 None:intergenic
GCTACTGCAATGATTAAGAT+GGG 0.493974 5_4:+47742028 Msa0854850:CDS
GGGAGATTAGAAGGCAATGC+AGG 0.503265 5_4:+47742080 Msa0854850:CDS
CCCAACAAAGATTCTTTGAG+AGG 0.507682 5_4:+47739105 Msa0854850:CDS
TAGTATAAGGCTTTAGAAGG+TGG 0.512062 5_4:-47737239 None:intergenic
AGCCTCTTACCGGATCAAAA+GGG 0.519613 5_4:+47742059 Msa0854850:CDS
TCAGCACATGAGACCACACC+GGG 0.520500 5_4:-47739174 None:intergenic
CACCACTGTTTGCATCATTG+AGG 0.529112 5_4:-47740946 None:intergenic
CAAAGCAGTCATGAAAATGA+AGG 0.532001 5_4:-47737468 None:intergenic
AGCTCAAGGACTCTCTACTA+AGG 0.542515 5_4:+47741018 Msa0854850:CDS
TATTGATAACAGGGTTGTGA+TGG 0.553791 5_4:+47739030 Msa0854850:intron
CCTATTGATAAGGTTGCTAA+GGG 0.554792 5_4:-47740990 None:intergenic
AACAGAATCACGAGCAGCAA+TGG 0.556484 5_4:-47739205 None:intergenic
AAAGCTCTTGATCAGAATGA+AGG 0.559330 5_4:-47741934 None:intergenic
ATAGTTTATTTGCCAAGTCA+AGG 0.563167 5_4:+47741789 Msa0854850:intron
ATAAGGCTTTAGAAGGTGGA+TGG 0.563249 5_4:-47737235 None:intergenic
AGGAATTGTCCAAGGAAAAG+TGG 0.565194 5_4:+47741815 Msa0854850:CDS
AGGTTGCTAAGGGTAGAAAA+GGG 0.568432 5_4:-47740980 None:intergenic
CTAAGGGTAGAAAAGGGAGG+TGG 0.578099 5_4:-47740974 None:intergenic
TATTTGCCAAGTCAAGGCAG+AGG 0.591700 5_4:+47741795 Msa0854850:CDS
GCTAGAGAACCTCGCATGGG+AGG 0.612577 5_4:+47737433 Msa0854850:CDS
TAAGTCTTTAGGATTAGGTG+AGG 0.616809 5_4:+47737157 None:intergenic
CAAGGCAGAGGAATTGTCCA+AGG 0.617740 5_4:+47741807 Msa0854850:CDS
TTGCTAAGGGTAGAAAAGGG+AGG 0.621073 5_4:-47740977 None:intergenic
TGTTGCTAGAGAACCTCGCA+TGG 0.621286 5_4:+47737429 Msa0854850:CDS
GCCTCTTACCGGATCAAAAG+GGG 0.621930 5_4:+47742060 Msa0854850:CDS
TGATGCCATAAAGTCTAAGG+TGG 0.622773 5_4:+47739140 Msa0854850:CDS
AAGGTGGAGTCATTATGCCC+CGG 0.647987 5_4:+47739156 Msa0854850:CDS
AGAAGAGATCCTCCCATGCG+AGG 0.655274 5_4:-47737442 None:intergenic
GTTGCTAGAGAACCTCGCAT+GGG 0.656226 5_4:+47737430 Msa0854850:CDS
AAGTCTTTAGGATTAGGTGA+GGG 0.660885 5_4:+47737158 None:intergenic
GGAGTCATTATGCCCCGGTG+TGG 0.661070 5_4:+47739161 Msa0854850:CDS
ATCAGCACATGAGACCACAC+CGG 0.662563 5_4:-47739175 None:intergenic
AGGACTCTCTACTAAGGACA+TGG 0.666154 5_4:+47741024 Msa0854850:CDS
AGCCTCAATGATGCAAACAG+TGG 0.715380 5_4:+47740944 Msa0854850:CDS
AGCAACAGCAGAGTGAACAA+CGG 0.720096 5_4:-47737413 None:intergenic
ACTCTCTACTAAGGACATGG+TGG 0.722310 5_4:+47741027 Msa0854850:CDS
CAGCACATGAGACCACACCG+GGG 0.839989 5_4:-47739173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTGAATATATTATTTTT+TGG + chr5_4:47737997-47738016 Msa0854850:intron 10.0%
!! AATTTATTAGATAAAGATTA+AGG - chr5_4:47738464-47738483 None:intergenic 10.0%
!! TAATCTTTATCTAATAAATT+TGG + chr5_4:47738464-47738483 Msa0854850:intron 10.0%
!!! AATTAATTTTTTTTAAATGG+TGG - chr5_4:47739991-47740010 None:intergenic 10.0%
!!! CACATATTTTTATTTTATTT+TGG + chr5_4:47740132-47740151 Msa0854850:intron 10.0%
!!! TTGTTTCATATTTTATAATA+GGG + chr5_4:47740156-47740175 Msa0854850:intron 10.0%
!! AGAAATAAAAAAAAATATTG+AGG + chr5_4:47740307-47740326 Msa0854850:intron 10.0%
!! GAAATAAAAAAAAATATTGA+GGG + chr5_4:47740308-47740327 Msa0854850:intron 10.0%
!! CATTATATATAGAGATAATA+TGG - chr5_4:47737193-47737212 None:intergenic 15.0%
!!! TGATCTATAATTTGTAAAAT+GGG + chr5_4:47739348-47739367 Msa0854850:intron 15.0%
!!! TTTTATTGAAGAGATTTTAA+TGG + chr5_4:47739596-47739615 Msa0854850:intron 15.0%
!! ATTAATTCATAAATATGTGT+TGG - chr5_4:47739719-47739738 None:intergenic 15.0%
!!! ATGTATATTTTAACATAAGA+GGG - chr5_4:47739824-47739843 None:intergenic 15.0%
!!! GTATAAATTTTACTTTTCAA+GGG - chr5_4:47739852-47739871 None:intergenic 15.0%
!!! AGTATAAATTTTACTTTTCA+AGG - chr5_4:47739853-47739872 None:intergenic 15.0%
!! ATATTTAATTATTAAGGAAG+GGG - chr5_4:47739906-47739925 None:intergenic 15.0%
!! CATATTTAATTATTAAGGAA+GGG - chr5_4:47739907-47739926 None:intergenic 15.0%
!! TTATTAAATACAATAAAGCA+TGG - chr5_4:47740107-47740126 None:intergenic 15.0%
!!! GTTGTTTCATATTTTATAAT+AGG + chr5_4:47740155-47740174 Msa0854850:intron 15.0%
!!! TATAATATGTTTTGAATAAG+AGG + chr5_4:47740409-47740428 Msa0854850:intron 15.0%
!!! ATAATATGTTTTGAATAAGA+GGG + chr5_4:47740410-47740429 Msa0854850:intron 15.0%
!! TATTAGAAGAAAAGAAATTA+GGG - chr5_4:47740804-47740823 None:intergenic 15.0%
!! TTATTAGAAGAAAAGAAATT+AGG - chr5_4:47740805-47740824 None:intergenic 15.0%
!! TAATAATAAAAGTAAGAAGA+AGG - chr5_4:47741102-47741121 None:intergenic 15.0%
!!! AATAATTTCATGTATTTTCT+TGG - chr5_4:47741294-47741313 None:intergenic 15.0%
!! AAGAAAATACATGAAATTAT+TGG + chr5_4:47741293-47741312 Msa0854850:intron 15.0%
!! TGTTGAAAATGAAATTTAAA+CGG - chr5_4:47741364-47741383 None:intergenic 15.0%
!!! TTCATGTTTTTTCTTATAGA+TGG + chr5_4:47737322-47737341 Msa0854850:CDS 20.0%
!! GTGGATATATAATATAGAAA+TGG - chr5_4:47737716-47737735 None:intergenic 20.0%
!!! ATTTCTTCCTCAATAAATTT+TGG + chr5_4:47737837-47737856 Msa0854850:intron 20.0%
!!! TTGAGATATGTGTTTTTTTT+TGG + chr5_4:47738383-47738402 Msa0854850:intron 20.0%
!!! TGAAAGTTTTTTAGTAGTAA+TGG + chr5_4:47738516-47738535 Msa0854850:intron 20.0%
!!! AACAACAATACTTTTTTTAG+AGG + chr5_4:47738541-47738560 Msa0854850:intron 20.0%
!!! ACAACAATACTTTTTTTAGA+GGG + chr5_4:47738542-47738561 Msa0854850:intron 20.0%
!!! AAAAGATTAATTTGTACGTA+CGG - chr5_4:47738576-47738595 None:intergenic 20.0%
!! TTAAATATCAAGCTTCAAAA+AGG - chr5_4:47738667-47738686 None:intergenic 20.0%
!! TGAATCATATGATTTAAATG+TGG + chr5_4:47738881-47738900 Msa0854850:intron 20.0%
!!! CATATGTTTATGAAGTTTTA+GGG - chr5_4:47738972-47738991 None:intergenic 20.0%
!!! TCATATGTTTATGAAGTTTT+AGG - chr5_4:47738973-47738992 None:intergenic 20.0%
!!! ACTTCATAAACATATGATTT+TGG + chr5_4:47738976-47738995 Msa0854850:intron 20.0%
!!! CTGATCTATAATTTGTAAAA+TGG + chr5_4:47739347-47739366 Msa0854850:intron 20.0%
!! AATATAATGTTTACAAGTCT+TGG + chr5_4:47739429-47739448 Msa0854850:intron 20.0%
!!! ATTTTTTGCTGATAATTTTC+TGG - chr5_4:47739457-47739476 None:intergenic 20.0%
!!! TGTATATTTTAACATAAGAG+GGG - chr5_4:47740441-47740460 None:intergenic 20.0%
!!! GATGTATATTTTAACATAAG+AGG - chr5_4:47739825-47739844 None:intergenic 20.0%
!! GTAATTTAAGCATCATATAA+GGG - chr5_4:47739881-47739900 None:intergenic 20.0%
!! TGTAATTTAAGCATCATATA+AGG - chr5_4:47739882-47739901 None:intergenic 20.0%
!! GCATATTTAATTATTAAGGA+AGG - chr5_4:47739908-47739927 None:intergenic 20.0%
!! TAGCGCATATTTAATTATTA+AGG - chr5_4:47739912-47739931 None:intergenic 20.0%
!! ATTTCGAAAAAAATAGTATG+CGG - chr5_4:47740254-47740273 None:intergenic 20.0%
!!! TAATATGTTTTGAATAAGAG+GGG + chr5_4:47740411-47740430 Msa0854850:intron 20.0%
!!! AGTGTATATTTTAACATAAG+AGG + chr5_4:47740439-47740458 Msa0854850:intron 20.0%
!!! GTGTATATTTTAACATAAGA+GGG + chr5_4:47740440-47740459 Msa0854850:intron 20.0%
!!! TGTATATTTTAACATAAGAG+GGG + chr5_4:47740441-47740460 Msa0854850:intron 20.0%
!!! TTTTTTTTGAAGAAAGAAGT+TGG + chr5_4:47740625-47740644 Msa0854850:intron 20.0%
!!! AAGTTTTAATTAGACACAAT+AGG - chr5_4:47741076-47741095 None:intergenic 20.0%
!!! TTACTATTTCATTTTAACTG+TGG - chr5_4:47741339-47741358 None:intergenic 20.0%
!!! ATTTTCTCTTTAATATCGAA+GGG - chr5_4:47741533-47741552 None:intergenic 20.0%
!! TTCGATATTAAAGAGAAAAT+GGG + chr5_4:47741533-47741552 Msa0854850:intron 20.0%
!! GTAAATATGATCTCTATAAA+CGG - chr5_4:47741753-47741772 None:intergenic 20.0%
!! TATAAGTTATCAAAATGGTT+TGG - chr5_4:47741884-47741903 None:intergenic 20.0%
!! TGTAGTATAAGTTATCAAAA+TGG - chr5_4:47741889-47741908 None:intergenic 20.0%
! TTTACAGCCTTAAAAACATT+GGG - chr5_4:47737391-47737410 None:intergenic 25.0%
! ATTTACAGCCTTAAAAACAT+TGG - chr5_4:47737392-47737411 None:intergenic 25.0%
! TTAGTATTTGATCATTGCAA+TGG - chr5_4:47737561-47737580 None:intergenic 25.0%
! CATCATCATTAAATTTGTGA+CGG - chr5_4:47737635-47737654 None:intergenic 25.0%
! GAAATCTTCATATGAACTAT+TGG - chr5_4:47737944-47737963 None:intergenic 25.0%
! TCTTATTGAGTGAATTGAAT+AGG + chr5_4:47738053-47738072 Msa0854850:intron 25.0%
! CTTATTGAGTGAATTGAATA+GGG + chr5_4:47738054-47738073 Msa0854850:intron 25.0%
!!! CAACAATACTTTTTTTAGAG+GGG + chr5_4:47738543-47738562 Msa0854850:intron 25.0%
!!! ACTTTTTGCTTATTGATAAC+AGG + chr5_4:47739020-47739039 Msa0854850:intron 25.0%
!!! CTTTTTGCTTATTGATAACA+GGG + chr5_4:47739021-47739040 Msa0854850:intron 25.0%
!! AATATGTGCATATAACTTGT+TGG - chr5_4:47739569-47739588 None:intergenic 25.0%
!!! AAATTTTACTTTTCAAGGGA+GGG - chr5_4:47739848-47739867 None:intergenic 25.0%
!!! TAAATTTTACTTTTCAAGGG+AGG - chr5_4:47739849-47739868 None:intergenic 25.0%
! TTTCGAAAAAAATAGTATGC+GGG - chr5_4:47740253-47740272 None:intergenic 25.0%
!!! AGTGTAGATTTTAACATAAG+AGG + chr5_4:47740336-47740355 Msa0854850:intron 25.0%
!!! GTGTAGATTTTAACATAAGA+GGG + chr5_4:47740337-47740356 Msa0854850:intron 25.0%
!!! TGTAGATTTTAACATAAGAG+GGG + chr5_4:47740338-47740357 Msa0854850:intron 25.0%
! CTTGATATATATCACACATT+TGG + chr5_4:47741148-47741167 Msa0854850:intron 25.0%
! CTTAGAACAAAACGAAAATT+AGG - chr5_4:47741221-47741240 None:intergenic 25.0%
!! ATTTCATGTATTTTCTTGGT+TGG - chr5_4:47741290-47741309 None:intergenic 25.0%
!! AATGCTATGTAAGTTGAATT+TGG - chr5_4:47741490-47741509 None:intergenic 25.0%
!! CATTTTCTCTTTAATATCGA+AGG - chr5_4:47741534-47741553 None:intergenic 25.0%
! CTTCGATATTAAAGAGAAAA+TGG + chr5_4:47741532-47741551 Msa0854850:intron 25.0%
!! CTGAAGTGATTAATGAATTT+TGG - chr5_4:47741670-47741689 None:intergenic 25.0%
! TTTAATTCTTTGTGCTTCTT+AGG + chr5_4:47741698-47741717 Msa0854850:intron 25.0%
! AAGAATCTCATCAACAAAAA+GGG + chr5_4:47741908-47741927 Msa0854850:CDS 25.0%
!! AAGTAGTATAAGGCTTTAGA+AGG - chr5_4:47737245-47737264 None:intergenic 30.0%
! CCATTTTGCTAAGTAGTATA+AGG - chr5_4:47737255-47737274 None:intergenic 30.0%
CCTTATACTACTTAGCAAAA+TGG + chr5_4:47737252-47737271 Msa0854850:exon 30.0%
!!! TTTCTCACTAGTTTTCTAAG+TGG - chr5_4:47737738-47737757 None:intergenic 30.0%
ATTAGCGCCAAAATTTATTG+AGG - chr5_4:47737847-47737866 None:intergenic 30.0%
!! TCTTTCAAAGAGTCTTATTG+AGG + chr5_4:47738144-47738163 Msa0854850:intron 30.0%
!! CTTTCAAAGAGTCTTATTGA+GGG + chr5_4:47738145-47738164 Msa0854850:intron 30.0%
TATATTCACCAACCTATGTT+GGG - chr5_4:47738286-47738305 None:intergenic 30.0%
ATATATTCACCAACCTATGT+TGG - chr5_4:47738287-47738306 None:intergenic 30.0%
ACAACATGGGAAAATTACAA+GGG - chr5_4:47738415-47738434 None:intergenic 30.0%
GAGTATTTAACTCACAACAT+GGG - chr5_4:47738428-47738447 None:intergenic 30.0%
TGCATTGTGAATAAAGCAAA+AGG + chr5_4:47738626-47738645 Msa0854850:intron 30.0%
!!! AAGCTTCAAAAAGGCATTTT+TGG - chr5_4:47738658-47738677 None:intergenic 30.0%
AAAATCAGACATGCTAAATG+TGG - chr5_4:47738827-47738846 None:intergenic 30.0%
!! AGCATGTCTGATTTTTGTTT+TGG + chr5_4:47738832-47738851 Msa0854850:intron 30.0%
TATTGATGCCATAAAGTCTA+AGG + chr5_4:47739137-47739156 Msa0854850:CDS 30.0%
! CTTTTAACATGCAATGTACA+TGG - chr5_4:47739695-47739714 None:intergenic 30.0%
TGTAATTCAAGCATCTTTGA+GGG - chr5_4:47740471-47740490 None:intergenic 30.0%
!! AATTTTACTTTTCAAGGGAG+GGG - chr5_4:47739847-47739866 None:intergenic 30.0%
TTTAAGCATCATATAAGGGT+GGG - chr5_4:47739877-47739896 None:intergenic 30.0%
ATTTAAGCATCATATAAGGG+TGG - chr5_4:47739878-47739897 None:intergenic 30.0%
ATGTAATTCAAGCTTCTCTT+AGG + chr5_4:47740367-47740386 Msa0854850:intron 30.0%
TGTAATTCAAGCTTCTCTTA+GGG + chr5_4:47740368-47740387 Msa0854850:intron 30.0%
!!! ATATTTTAACATAAGAGGGG+AGG + chr5_4:47740444-47740463 Msa0854850:intron 30.0%
TGTAATTCAAGCATCTTTGA+AGG + chr5_4:47740471-47740490 Msa0854850:intron 30.0%
GTAATTCAAGCATCTTTGAA+GGG + chr5_4:47740472-47740491 Msa0854850:intron 30.0%
TTCGAAGAATCTATTTCGTT+TGG + chr5_4:47740534-47740553 Msa0854850:intron 30.0%
TGGTGAAAAAAAAGAGAAGT+TGG + chr5_4:47740595-47740614 Msa0854850:intron 30.0%
! TTCTATCATATTTTGCAGCT+TGG + chr5_4:47740872-47740891 Msa0854850:intron 30.0%
!! GAGCTTTAAACCTATTGATA+AGG - chr5_4:47741003-47741022 None:intergenic 30.0%
! ATCAATAGGTTTAAAGCTCA+AGG + chr5_4:47741004-47741023 Msa0854850:CDS 30.0%
AAAAAGACATGTACCAGATA+AGG - chr5_4:47741053-47741072 None:intergenic 30.0%
TTTAGATTATCAAACCACCA+GGG - chr5_4:47741560-47741579 None:intergenic 30.0%
ATAGTTTATTTGCCAAGTCA+AGG + chr5_4:47741789-47741808 Msa0854850:intron 30.0%
!! TTTATTGTACCACTTTTCCT+TGG - chr5_4:47741827-47741846 None:intergenic 30.0%
CAAGAATCTCATCAACAAAA+AGG + chr5_4:47741907-47741926 Msa0854850:CDS 30.0%
!! TAGTATAAGGCTTTAGAAGG+TGG - chr5_4:47737242-47737261 None:intergenic 35.0%
!! GAAGTGTCCCAATGTTTTTA+AGG + chr5_4:47737381-47737400 Msa0854850:CDS 35.0%
CAAAGCAGTCATGAAAATGA+AGG - chr5_4:47737471-47737490 None:intergenic 35.0%
!! GTTTTCTTTCTCTGTTTCTC+TGG - chr5_4:47737593-47737612 None:intergenic 35.0%
ATCATCACACTATTTAGTGC+TGG - chr5_4:47737798-47737817 None:intergenic 35.0%
GGACGTAACTATTGCAATAA+TGG + chr5_4:47738166-47738185 Msa0854850:intron 35.0%
ATATTCACCAACCTATGTTG+GGG - chr5_4:47738285-47738304 None:intergenic 35.0%
CACAACATGGGAAAATTACA+AGG - chr5_4:47738416-47738435 None:intergenic 35.0%
CGAGTATTTAACTCACAACA+TGG - chr5_4:47738429-47738448 None:intergenic 35.0%
ACGCATGTCTAACTAATATG+CGG - chr5_4:47738752-47738771 None:intergenic 35.0%
AGGGCAATGATAGGTATATA+AGG - chr5_4:47738953-47738972 None:intergenic 35.0%
!!! TGAAGTTTTAGGGCAATGAT+AGG - chr5_4:47738962-47738981 None:intergenic 35.0%
! TATTGATAACAGGGTTGTGA+TGG + chr5_4:47739030-47739049 Msa0854850:intron 35.0%
TTCTTCTCTCCTTTATTGGA+GGG - chr5_4:47739078-47739097 None:intergenic 35.0%
ATTCTTCTCTCCTTTATTGG+AGG - chr5_4:47739079-47739098 None:intergenic 35.0%
TGCATTCTTCTCTCCTTTAT+TGG - chr5_4:47739082-47739101 None:intergenic 35.0%
CCTCTCAAAGAATCTTTGTT+GGG - chr5_4:47739108-47739127 None:intergenic 35.0%
ACCTCTCAAAGAATCTTTGT+TGG - chr5_4:47739109-47739128 None:intergenic 35.0%
GTAATTCAAGCATCTTTGAG+GGG - chr5_4:47739792-47739811 None:intergenic 35.0%
GTGTAATTCAAGCATCTTTG+AGG - chr5_4:47740470-47740489 None:intergenic 35.0%
TTAAGCATCATATAAGGGTG+GGG - chr5_4:47739876-47739895 None:intergenic 35.0%
!! AGATTTTAACATAAGAGGGG+AGG + chr5_4:47740341-47740360 Msa0854850:intron 35.0%
!! GATTTTAACATAAGAGGGGA+GGG + chr5_4:47740342-47740361 Msa0854850:intron 35.0%
!! ATTTTAACATAAGAGGGGAG+GGG + chr5_4:47740343-47740362 Msa0854850:intron 35.0%
GTAATTCAAGCTTCTCTTAG+GGG + chr5_4:47740369-47740388 Msa0854850:intron 35.0%
!! TATGTTTTGAATAAGAGGGG+AGG + chr5_4:47740414-47740433 Msa0854850:intron 35.0%
!! ATGTTTTGAATAAGAGGGGA+GGG + chr5_4:47740415-47740434 Msa0854850:intron 35.0%
GAATCTATTTCGTTTGGCAA+TGG + chr5_4:47740540-47740559 Msa0854850:intron 35.0%
CGTTATGTTTAGCAAAATCG+CGG - chr5_4:47740684-47740703 None:intergenic 35.0%
! TATCATATTTTGCAGCTTGG+AGG + chr5_4:47740875-47740894 Msa0854850:intron 35.0%
! ATCATATTTTGCAGCTTGGA+GGG + chr5_4:47740876-47740895 Msa0854850:intron 35.0%
CCAAGTTTAACCTTCCAAAA+AGG - chr5_4:47740902-47740921 None:intergenic 35.0%
!! CCTTTTTGGAAGGTTAAACT+TGG + chr5_4:47740899-47740918 Msa0854850:CDS 35.0%
GAAGGTTAAACTTGGAAGAA+GGG + chr5_4:47740907-47740926 Msa0854850:CDS 35.0%
CCTATTGATAAGGTTGCTAA+GGG - chr5_4:47740993-47741012 None:intergenic 35.0%
ACCTATTGATAAGGTTGCTA+AGG - chr5_4:47740994-47741013 None:intergenic 35.0%
TATCACACATTTGGTTGACT+AGG + chr5_4:47741157-47741176 Msa0854850:intron 35.0%
CTTTAGATTATCAAACCACC+AGG - chr5_4:47741561-47741580 None:intergenic 35.0%
AAAGCTCTTGATCAGAATGA+AGG - chr5_4:47741937-47741956 None:intergenic 35.0%
! TCTGATCAAGAGCTTTTCAA+TGG + chr5_4:47741941-47741960 Msa0854850:CDS 35.0%
TGCTACTGCAATGATTAAGA+TGG + chr5_4:47742027-47742046 Msa0854850:CDS 35.0%
GCTACTGCAATGATTAAGAT+GGG + chr5_4:47742028-47742047 Msa0854850:CDS 35.0%
!! ATAAGGCTTTAGAAGGTGGA+TGG - chr5_4:47737238-47737257 None:intergenic 40.0%
!!! CTCACTAGTTTTCTAAGTGG+TGG - chr5_4:47737735-47737754 None:intergenic 40.0%
! TTTTCATGTCGTTACGCTAC+TGG + chr5_4:47738087-47738106 Msa0854850:intron 40.0%
AAATCGCTCTCAAAGATGAG+TGG - chr5_4:47738255-47738274 None:intergenic 40.0%
ATTTACCATGTCCCCAACAT+AGG + chr5_4:47738271-47738290 Msa0854850:intron 40.0%
CCCAACAAAGATTCTTTGAG+AGG + chr5_4:47739105-47739124 Msa0854850:CDS 40.0%
TGATGCCATAAAGTCTAAGG+TGG + chr5_4:47739140-47739159 Msa0854850:CDS 40.0%
TGACTCCACCTTAGACTTTA+TGG - chr5_4:47739148-47739167 None:intergenic 40.0%
AGAGACTGTAGACTAAGATG+TGG + chr5_4:47739512-47739531 Msa0854850:intron 40.0%
!! TGTTTTGAATAAGAGGGGAG+GGG + chr5_4:47740416-47740435 Msa0854850:intron 40.0%
ATAGACGTTTCAACGAGTCA+TGG + chr5_4:47740572-47740591 Msa0854850:intron 40.0%
GGAAGGTTAAACTTGGAAGA+AGG + chr5_4:47740906-47740925 Msa0854850:CDS 40.0%
AGGTTGCTAAGGGTAGAAAA+GGG - chr5_4:47740983-47741002 None:intergenic 40.0%
AAGGTTGCTAAGGGTAGAAA+AGG - chr5_4:47740984-47741003 None:intergenic 40.0%
CCCTTAGCAACCTTATCAAT+AGG + chr5_4:47740990-47741009 Msa0854850:CDS 40.0%
TTAAAGAGAAAATGGGACCC+TGG + chr5_4:47741540-47741559 Msa0854850:intron 40.0%
TTCTTAGGAGCTCACACAAT+TGG + chr5_4:47741713-47741732 Msa0854850:intron 40.0%
AGGAATTGTCCAAGGAAAAG+TGG + chr5_4:47741815-47741834 Msa0854850:CDS 40.0%
!!! GGTTTGGTGTTTTGAAGTCT+AGG - chr5_4:47741868-47741887 None:intergenic 40.0%
! GATCAAGAGCTTTTCAATGG+AGG + chr5_4:47741944-47741963 Msa0854850:CDS 40.0%
!! TGGAGGATCTACTGATTCAT+TGG + chr5_4:47741961-47741980 Msa0854850:CDS 40.0%
GGAAATAACAAGCCTCTTAC+CGG + chr5_4:47742049-47742068 Msa0854850:CDS 40.0%
! TCTAATCTCCCCTTTTGATC+CGG - chr5_4:47742071-47742090 None:intergenic 40.0%
GATCAAAAGGGGAGATTAGA+AGG + chr5_4:47742071-47742090 Msa0854850:CDS 40.0%
AGCAACAGCAGAGTGAACAA+CGG - chr5_4:47737416-47737435 None:intergenic 45.0%
GATGACACACCCTCCAATAA+AGG + chr5_4:47739066-47739085 Msa0854850:CDS 45.0%
AACAGAATCACGAGCAGCAA+TGG - chr5_4:47739208-47739227 None:intergenic 45.0%
TCTACAGTCTCTCTACATCG+AGG - chr5_4:47739504-47739523 None:intergenic 45.0%
! TTCAAGCATCTTTGAGGGGA+GGG - chr5_4:47739788-47739807 None:intergenic 45.0%
! ATTCAAGCATCTTTGAGGGG+AGG - chr5_4:47739789-47739808 None:intergenic 45.0%
ATTCAAGCTTCTCTTAGGGG+AGG + chr5_4:47740372-47740391 Msa0854850:intron 45.0%
TTCAAGCTTCTCTTAGGGGA+GGG + chr5_4:47740373-47740392 Msa0854850:intron 45.0%
ACTGTTTGCATCATTGAGGC+TGG - chr5_4:47740945-47740964 None:intergenic 45.0%
AGCCTCAATGATGCAAACAG+TGG + chr5_4:47740944-47740963 Msa0854850:CDS 45.0%
CACCACTGTTTGCATCATTG+AGG - chr5_4:47740949-47740968 None:intergenic 45.0%
TTGCTAAGGGTAGAAAAGGG+AGG - chr5_4:47740980-47740999 None:intergenic 45.0%
AGCTCAAGGACTCTCTACTA+AGG + chr5_4:47741018-47741037 Msa0854850:CDS 45.0%
AGGACTCTCTACTAAGGACA+TGG + chr5_4:47741024-47741043 Msa0854850:CDS 45.0%
ACTCTCTACTAAGGACATGG+TGG + chr5_4:47741027-47741046 Msa0854850:CDS 45.0%
!! GTACTCTGACTCTAGAGTCT+TGG + chr5_4:47741388-47741407 Msa0854850:intron 45.0%
AGGAGCTCACACAATTGGAA+AGG + chr5_4:47741718-47741737 Msa0854850:intron 45.0%
CTCACACAATTGGAAAGGCA+AGG + chr5_4:47741723-47741742 Msa0854850:intron 45.0%
TATTTGCCAAGTCAAGGCAG+AGG + chr5_4:47741795-47741814 Msa0854850:CDS 45.0%
ACAATTCCTCTGCCTTGACT+TGG - chr5_4:47741804-47741823 None:intergenic 45.0%
AAGCCTCTTACCGGATCAAA+AGG + chr5_4:47742058-47742077 Msa0854850:CDS 45.0%
AGCCTCTTACCGGATCAAAA+GGG + chr5_4:47742059-47742078 Msa0854850:CDS 45.0%
!!! ACAAAAATTTAATAATTTTT+TGG - chr5_4:47738326-47738345 None:intergenic 5.0%
!!! ATGAATTAATTTTTTTTAAA+TGG - chr5_4:47739994-47740013 None:intergenic 5.0%
!!! ACATATTTTTATTTTATTTT+GGG + chr5_4:47740133-47740152 Msa0854850:intron 5.0%
TGTTGCTAGAGAACCTCGCA+TGG + chr5_4:47737429-47737448 Msa0854850:CDS 50.0%
GTTGCTAGAGAACCTCGCAT+GGG + chr5_4:47737430-47737449 Msa0854850:CDS 50.0%
ACCATGTCCCCAACATAGGT+TGG + chr5_4:47738275-47738294 Msa0854850:intron 50.0%
ACCAACCTATGTTGGGGACA+TGG - chr5_4:47738279-47738298 None:intergenic 50.0%
TTTGTGAAGTCACGCGCACA+CGG - chr5_4:47738799-47738818 None:intergenic 50.0%
AAGGTGGAGTCATTATGCCC+CGG + chr5_4:47739156-47739175 Msa0854850:CDS 50.0%
ATCAGCACATGAGACCACAC+CGG - chr5_4:47739178-47739197 None:intergenic 50.0%
! TCAAGCATCTTTGAGGGGAG+GGG - chr5_4:47739787-47739806 None:intergenic 50.0%
TCAAGCTTCTCTTAGGGGAG+GGG + chr5_4:47740374-47740393 Msa0854850:intron 50.0%
GACGTTTCAACGAGTCATGG+TGG + chr5_4:47740575-47740594 Msa0854850:intron 50.0%
CTAAGGGTAGAAAAGGGAGG+TGG - chr5_4:47740977-47740996 None:intergenic 50.0%
!! AAGGACATGGTGGCCTTATC+TGG + chr5_4:47741037-47741056 Msa0854850:CDS 50.0%
GGCATGACATGAAGTCTACG+TGG - chr5_4:47741200-47741219 None:intergenic 50.0%
AAGAGAAAATGGGACCCTGG+TGG + chr5_4:47741543-47741562 Msa0854850:intron 50.0%
CAAGGCAGAGGAATTGTCCA+AGG + chr5_4:47741807-47741826 Msa0854850:CDS 50.0%
GCCTCTTACCGGATCAAAAG+GGG + chr5_4:47742060-47742079 Msa0854850:CDS 50.0%
! TCCCCTTTTGATCCGGTAAG+AGG - chr5_4:47742064-47742083 None:intergenic 50.0%
GGGAGATTAGAAGGCAATGC+AGG + chr5_4:47742080-47742099 Msa0854850:CDS 50.0%
AGAAGAGATCCTCCCATGCG+AGG - chr5_4:47737445-47737464 None:intergenic 55.0%
TCAGCACATGAGACCACACC+GGG - chr5_4:47739177-47739196 None:intergenic 55.0%
! CAAGCATCTTTGAGGGGAGG+GGG - chr5_4:47739786-47739805 None:intergenic 55.0%
! TGCAGCTTGGAGGGCCTTTT+TGG + chr5_4:47740885-47740904 Msa0854850:intron 55.0%
! GCTTGGAGGGCCTTTTTGGA+AGG + chr5_4:47740889-47740908 Msa0854850:intron 55.0%
GCTAGAGAACCTCGCATGGG+AGG + chr5_4:47737433-47737452 Msa0854850:CDS 60.0%
GGAGTCATTATGCCCCGGTG+TGG + chr5_4:47739161-47739180 Msa0854850:CDS 60.0%
CAGCACATGAGACCACACCG+GGG - chr5_4:47739176-47739195 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 47737164 47742114 47737164 ID=Msa0854850;Name=Msa0854850
chr5_4 mRNA 47737164 47742114 47737164 ID=Msa0854850-mRNA-1;Parent=Msa0854850;Name=Msa0854850-mRNA-1;_AED=0.19;_eAED=0.19;_QI=107|0.66|0.5|1|0.66|0.75|4|0|309
chr5_4 exon 47737164 47737495 47737164 ID=Msa0854850-mRNA-1:exon:15050;Parent=Msa0854850-mRNA-1
chr5_4 exon 47739042 47739233 47739042 ID=Msa0854850-mRNA-1:exon:15051;Parent=Msa0854850-mRNA-1
chr5_4 exon 47740890 47741080 47740890 ID=Msa0854850-mRNA-1:exon:15052;Parent=Msa0854850-mRNA-1
chr5_4 exon 47741793 47742114 47741793 ID=Msa0854850-mRNA-1:exon:15053;Parent=Msa0854850-mRNA-1
chr5_4 five_prime_UTR 47737164 47737270 47737164 ID=Msa0854850-mRNA-1:five_prime_utr;Parent=Msa0854850-mRNA-1
chr5_4 CDS 47737271 47737495 47737271 ID=Msa0854850-mRNA-1:cds;Parent=Msa0854850-mRNA-1
chr5_4 CDS 47739042 47739233 47739042 ID=Msa0854850-mRNA-1:cds;Parent=Msa0854850-mRNA-1
chr5_4 CDS 47740890 47741080 47740890 ID=Msa0854850-mRNA-1:cds;Parent=Msa0854850-mRNA-1
chr5_4 CDS 47741793 47742114 47741793 ID=Msa0854850-mRNA-1:cds;Parent=Msa0854850-mRNA-1
Gene Sequence

>Msa0854850

ATGGCACTTTCTTCTTCTAAAGTTTCACTAAATTTTTTTTGCTTTGTTTTGTTCATGTTTTTTCTTATAGATGGTTCTTTTTCTCAACTATCTGAAAATTTCTATGCTAAGAAGTGTCCCAATGTTTTTAAGGCTGTAAATTCCGTTGTTCACTCTGCTGTTGCTAGAGAACCTCGCATGGGAGGATCTCTTCTTCGCCTTCATTTTCATGACTGCTTTGTTAATGGTTGTGATGGATCAGTGCTACTTGATGACACACCCTCCAATAAAGGAGAGAAGAATGCACTTCCCAACAAAGATTCTTTGAGAGGTTTTGAAGTTATTGATGCCATAAAGTCTAAGGTGGAGTCATTATGCCCCGGTGTGGTCTCATGTGCTGATATTTTAGCCATTGCTGCTCGTGATTCTGTTGTAAATCTTGGAGGGCCTTTTTGGAAGGTTAAACTTGGAAGAAGGGATTCAAAAACAGCCAGCCTCAATGATGCAAACAGTGGTGTAATTCCACCTCCCTTTTCTACCCTTAGCAACCTTATCAATAGGTTTAAAGCTCAAGGACTCTCTACTAAGGACATGGTGGCCTTATCTGGTACATGTCTTTTTCCTATTGTTTTATTTGCCAAGTCAAGGCAGAGGAATTGTCCAAGGAAAAGTGGTACAATAAAAGACAACAATGTAGCTGTCCTAGACTTCAAAACACCAAACCATTTTGATAACTTATACTACAAGAATCTCATCAACAAAAAGGGACTCCTTCATTCTGATCAAGAGCTTTTCAATGGAGGATCTACTGATTCATTGGTTAAAAGTTATAGCAATAATCAAAATGCATTTGAATCAGATTTTGCTACTGCAATGATTAAGATGGGAAATAACAAGCCTCTTACCGGATCAAAAGGGGAGATTAGAAGGCAATGCAGGAGAGCCAATTAA
Protein sequence

>Msa0854850

MALSSSKVSLNFFCFVLFMFFLIDGSFSQLSENFYAKKCPNVFKAVNSVVHSAVAREPRMGGSLLRLHFHDCFVNGCDGSVLLDDTPSNKGEKNALPNKDSLRGFEVIDAIKSKVESLCPGVVSCADILAIAARDSVVNLGGPFWKVKLGRRDSKTASLNDANSGVIPPPFSTLSNLINRFKAQGLSTKDMVALSGTCLFPIVLFAKSRQRNCPRKSGTIKDNNVAVLDFKTPNHFDNLYYKNLINKKGLLHSDQELFNGGSTDSLVKSYSNNQNAFESDFATAMIKMGNNKPLTGSKGEIRRQCRRAN*