AlfalfaGEDB | Msa0736110

M. sativa cultivar zhongmu-4 / Msa0736110

KEGG: K10706
GO: -
PFAM: PF20073.2

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736110	XP_024639454.1	92.912	917	64	1	1	916	5	921	0.0	1733

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736110	sp\|B6SFA4\|MAA3_ARATH	34.156	243	152	6	664	902	459	697	2.77e-31	135

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736110	A0A396HXY4	92.912	917	64	1	1	916	1	917	0.0	1732

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0448680	Msa0736110	0.804317	2.288781e-49	-8.615850e-47
Msa0557450	Msa0736110	0.827039	1.949685e-54	-8.615850e-47
Msa0736060	Msa0736110	0.832701	8.134366e-56	-8.615850e-47
Msa0736110	Msa0777930	0.808311	3.296129e-50	-8.615850e-47
Msa0736110	Msa0815180	0.822444	2.360764e-53	-8.615850e-47
Msa0736110	Msa0885270	0.800143	1.654897e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736110	MtrunA17_Chr5g0433421	92.912	917	64	1	1	916	1	917	0.0	1732
Msa0736110	MtrunA17_Chr5g0433511	75.425	529	71	6	1	484	2	516	0.0	748
Msa0736110	MtrunA17_Chr5g0433511	79.720	429	87	0	484	912	418	846	0.0	687
Msa0736110	MtrunA17_Chr3g0109551	40.649	925	426	25	17	902	13	853	0.0	601
Msa0736110	MtrunA17_Chr5g0433471	59.063	491	175	8	9	484	15	494	0.0	556
Msa0736110	MtrunA17_Chr5g0433471	64.896	433	136	4	483	902	388	817	1.02e-174	531
Msa0736110	MtrunA17_Chr5g0433351	67.172	396	119	3	1	385	1	396	1.68e-178	524
Msa0736110	MtrunA17_Chr5g0433351	68.519	54	14	1	561	611	417	470	6.64e-12	68.9
Msa0736110	MtrunA17_Chr5g0433361	60.460	435	119	8	1	385	1	432	6.92e-160	491
Msa0736110	MtrunA17_Chr5g0433361	66.856	353	101	4	561	902	451	798	2.10e-146	456

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736110	AT4G05540.1	49.580	357	162	5	553	902	301	646	1.92e-103	337
Msa0736110	AT4G05540.1	35.326	368	178	16	133	484	10	333	1.41e-42	166
Msa0736110	AT1G65810.3	49.444	360	160	5	552	902	420	766	1.81e-97	329
Msa0736110	AT1G65810.3	40.404	396	210	7	17	392	22	411	2.34e-81	284
Msa0736110	AT1G65810.2	49.444	360	160	5	552	902	420	766	2.10e-97	328
Msa0736110	AT1G65810.2	40.404	396	210	7	17	392	22	411	1.94e-81	284
Msa0736110	AT1G65810.1	49.444	360	160	5	552	902	420	766	2.69e-97	329
Msa0736110	AT1G65810.1	40.404	396	210	7	17	392	22	411	3.43e-81	284
Msa0736110	AT5G52090.1	47.309	353	181	3	552	902	230	579	4.24e-97	320
Msa0736110	AT5G52090.1	43.810	210	106	6	190	392	1	205	4.43e-43	167

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Find 167 sgRNAs with CRISPR-Local

Find 522 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGATATGGGGACCACC+AGG	0.527115	5_1:+52011387	Msa0736110:CDS
AAAGAATTTCTTAGACCTGA+AGG	0.489968	5_1:-52008330	None:intergenic
AAAGGAAACTTAGTATGTAA+AGG	0.529734	5_1:-52012475	None:intergenic
AACATCCATCTTCATGCATT+TGG	0.305607	5_1:-52010813	None:intergenic
AACTGATATGGGGACCACCA+GGG	0.682358	5_1:+52011388	Msa0736110:CDS
AACTGCAAAATTTGCATGTC+TGG	0.326165	5_1:+52011243	Msa0736110:CDS
AAGATAAATAAGGGGAACTA+TGG	0.408299	5_1:+52011813	Msa0736110:CDS
AAGCTTAATGCAGCTGCTTG+AGG	0.550630	5_1:+52012010	Msa0736110:CDS
AATACTACGCTTTGTGACAA+AGG	0.549299	5_1:+52008822	Msa0736110:five_prime_UTR
AATCTCTTCTCTTAGCATCA+AGG	0.554242	5_1:-52014199	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTATAGAATACTTCCTTC+AGG	+	chr5_1:52008315-52008334	Msa0736110:five_prime_UTR	30.0%
AAAGAATTTCTTAGACCTGA+AGG	-	chr5_1:52008333-52008352	None:intergenic	30.0%
ACACTAACATTGAAAGAAGA+AGG	-	chr5_1:52008907-52008926	None:intergenic	30.0%
GTGTATAAAATGAAATGGAG+TGG	+	chr5_1:52009099-52009118	Msa0736110:intron	30.0%
GTATAAAATGAAATGGAGTG+GGG	+	chr5_1:52009101-52009120	Msa0736110:intron	30.0%
AAAAATTAATCGAAACCGCA+CGG	+	chr5_1:52009528-52009547	Msa0736110:intron	30.0%
GTGAGGAAAAATATAAAGCA+TGG	+	chr5_1:52009568-52009587	Msa0736110:intron	30.0%
ATAACAGAACAGAATATGAG+AGG	-	chr5_1:52009620-52009639	None:intergenic	30.0%
AGACATATACTTCAACTGTT+TGG	+	chr5_1:52009745-52009764	Msa0736110:five_prime_UTR	30.0%
GAGAAAATCTAACAATGAAG+TGG	-	chr5_1:52010056-52010075	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	52008305	52017522	52008305	ID=Msa0736110;Name=Msa0736110
chr5_1	mRNA	52008305	52017522	52008305	ID=Msa0736110-mRNA-1;Parent=Msa0736110;Name=Msa0736110-mRNA-1;_AED=0.15;_eAED=0.15;_QI=368\|0.88\|0.9\|1\|0.55\|0.5\|10\|252\|916
chr5_1	exon	52008305	52008503	52008305	ID=Msa0736110-mRNA-1:exon:14685;Parent=Msa0736110-mRNA-1
chr5_1	exon	52008728	52008768	52008728	ID=Msa0736110-mRNA-1:exon:14686;Parent=Msa0736110-mRNA-1
chr5_1	exon	52008789	52008850	52008789	ID=Msa0736110-mRNA-1:exon:14687;Parent=Msa0736110-mRNA-1
chr5_1	exon	52009730	52009909	52009730	ID=Msa0736110-mRNA-1:exon:14688;Parent=Msa0736110-mRNA-1
chr5_1	exon	52010434	52010988	52010434	ID=Msa0736110-mRNA-1:exon:14689;Parent=Msa0736110-mRNA-1
chr5_1	exon	52011234	52012760	52011234	ID=Msa0736110-mRNA-1:exon:14690;Parent=Msa0736110-mRNA-1
chr5_1	exon	52013327	52013651	52013327	ID=Msa0736110-mRNA-1:exon:14691;Parent=Msa0736110-mRNA-1
chr5_1	exon	52013774	52013955	52013774	ID=Msa0736110-mRNA-1:exon:14692;Parent=Msa0736110-mRNA-1
chr5_1	exon	52014130	52014299	52014130	ID=Msa0736110-mRNA-1:exon:14693;Parent=Msa0736110-mRNA-1
chr5_1	exon	52017393	52017522	52017393	ID=Msa0736110-mRNA-1:exon:14694;Parent=Msa0736110-mRNA-1
chr5_1	five_prime_UTR	52008305	52008503	52008305	ID=Msa0736110-mRNA-1:five_prime_utr;Parent=Msa0736110-mRNA-1
chr5_1	five_prime_UTR	52008728	52008768	52008728	ID=Msa0736110-mRNA-1:five_prime_utr;Parent=Msa0736110-mRNA-1
chr5_1	five_prime_UTR	52008789	52008850	52008789	ID=Msa0736110-mRNA-1:five_prime_utr;Parent=Msa0736110-mRNA-1
chr5_1	five_prime_UTR	52009730	52009795	52009730	ID=Msa0736110-mRNA-1:five_prime_utr;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52009796	52009909	52009796	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52010434	52010988	52010434	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52011234	52012760	52011234	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52013327	52013651	52013327	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52013774	52013955	52013774	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	CDS	52014130	52014177	52014130	ID=Msa0736110-mRNA-1:cds;Parent=Msa0736110-mRNA-1
chr5_1	three_prime_UTR	52014178	52014299	52014178	ID=Msa0736110-mRNA-1:three_prime_utr;Parent=Msa0736110-mRNA-1
chr5_1	three_prime_UTR	52017393	52017522	52017393	ID=Msa0736110-mRNA-1:three_prime_utr;Parent=Msa0736110-mRNA-1

Gene Sequence

>Msa0736110

ATGGAGAAGACAAGAAATGGTAGTGAAGAGGATCTTAAACTCGGGAGTCTTATAAATATCGTACTCTCTTGGACCCTGGAGGATGTTCTCAATGAAAATCTTTACAAAGACAAGGTTCATAAGATTCCAGAGACATTTAAGTCAGCAACAGATTACAAGAACTCTTTTATTCCACTATTGTTTGAGGAAACACGCGCTGATTTATCTTCAAGCTTGTCTGGTGTGTCTCAAGCTGCTCTTTGTGAAATCAAGAATGTCGAACACAGCAAGCAGTTGAAACTTCGCAAAGCTCAAAATCAATTCATTCAGTTCCATCATACCATATGGTTGAAGAGTACAACTGATTATGAACCTGCTTCAGGAGATCTTATTGCTATTACATACATTAGGCCAAAAAGCTTAAATGACTTGAACACACTCAACAGTCCCTACCATATTGCCTATGTTAATGGGGAAAAAAATAGATTTTCTGGTAGAATCACAGTTCTATCTTCCAAATGCATGAAGATGGATGTTGAATCTGTATCCAGGAAAAACAATACACAGAAAATGTATGCTGTATACATAATGAACATGACAACAAATGTTCGTATTTGGAAAGCCTTGGACTCAAAGTCAAAGGGTGATCACCTCAACATTATTGAAAAAGTTTTGCAACCTAATCTCAATAGTGGAGAGAACTGCAAAATTTGCATGTCTGGATCAAATAGTCAAGCTTCTTTCATTACGAAAGACATAATTCAATCTCAGAATCTGAATGAATCCCAACAAGACGCAGTTACAAGCTGTGTTAGTATGGTAAATTGTTCTCATGCTAATACCAAACTGATATGGGGACCACCAGGGACAGGAAAAACAAAGACTGTTGCATGCTTGCTTTTTTCTCTACTCAAGTTGAAATCAAGAACATTGACCTGTGCTCCAACTAATACTGCAGTGTTGCAAGTGGCAACAAGGTTGCATAGTTTAGTTACAGGTTCACTTGACCATGATACATATGGTTTAGCTGACATTGTGCTATTTGGCAATGGTAAGAGAATGAAAGTGGATAGTTATCCAGGTCTTGAAGATATCTTTCTTGATTATCGCGTGAAAAATCTCATGCAGTGCTATGCTGAATGGAAGCATAGCTTTGAAGCAATGATCGAATTTCTTAGTGATCCTAGTAAACAATATTTCTTGGAGATGTCCAAAAAGGATTTTGGTATGGATAAAAATAGGATTTTAGCATCTGCATATCATGCATACAAGATAAATAAGGGGAACTATGGTCTGATTATGAGGTTTGAGGATTATGTTCAGAAGGCACGGACAGAGATCACTAAGCTATACCAATTGGATGAGAATGACAAAAAGGAATGTATGTTGACAATTGAGCAATTTGTGAAACAGAGAATTGATAAATTAAGCATGAATAGAGTCAATATCTTCATGACCTTATATACAAGCTTAATGCAGCTGCTTGAGGACCCTAGAGAGCAACTTTTCTCAAAGATGGGTTACAAGTCCCTTGATGATTTTGCTACAAATAGTATTGTAGTATCCGCTTATAGTGCTTACAAGCAAAATAAGGGGAGTGATAAATATGACAATACTTTGACTTTTGAGGGATATGTGAAGAGGGCAAGGAAAGAGATTATAGAGCTATACCAAAGTATCATGACCATGGAGCAATTTGTGAAGCAGAGATATTTAGAATTAAGAGAGAAGCTAAAGTTCTTGCTACTTACCTTATACACCCACATGCCAAAATCTTTCATTTCAGTGAACAATATTCTCCAAGCTCTTGACTCGTTAAAATCACTGGAAATTTCCTTGAGACAAGCCAAATTTAAGCAAGCTGTTGCTGATTGTGAAGAAGAGTGCATTCCTGTTTGCTTAGGACCATCAAGTTTGGAAAGAAAGGATTGCCTTTACATACTAAGTTTCCTTTCCAAGTCAATTTCGCTTCCTGATTTTAAAGTGAGACATCTAGTAGAAAAGTTTTGCTTGTCAAATGCTAGTCTAATTTTGTGTACTGTATCAAGTTCTATCAAACTTTACTCAGAAGAGAAGAGTCCGGTGACATTTTTAGTTATTGATGAAGCAGCTATGCTAAAAGAATGCGAGTCAACCATTCCATTGCAGCTTCCTGGTCTCTGTCATTGTATCCTAATAGGTGATGAGAGGCAACTTCCTGCATTGGTAAAAAGCAAGATTGCAGACGAGTGTGATTTTGGACGAAGTATGTTCGAGAGGCTAGTTACTTCAGGCTACAAGAGGCATATGCTTAATGTTCAGTATAGAATGCATCCATCAATTAGCTTATTTCCATGCAAAGAGTTCTATGATGGAAAAATTTCTGATGCCGTCATTGTTAGGAAAGAAAAATATAATAAGCATTTCCTTCAAGGAAAAATGTATGCTTCTTATTCTTTTATTAACATAGCCAAGGGAAAAGAGCAATTTGGTTGCGGAAACAGTTTGAAGAACATGGTTGAGGTAGCTGTTATTTCCAAGATACTTGAAAGCCTAAAACATGAATTTATGAGGACAAAGAAGAAAGTGAGCATAGGCATCATATCTCCATATAATGCTCAAGTTTTTGAAATTCAAGAAAAAGTTAAGCAATACATTGCCGTTTCTGATACCGACTTCTCTGTTAGTGTCCGTTCTGTTGATGGTTCTCAAGGTGGTGAGGAAGATATAATAATAATATCAACTATATTGCCTTTGGATATTAGGAAATGCAGCTACTCTAATCAACAGTGA

Protein sequence

>Msa0736110

MEKTRNGSEEDLKLGSLINIVLSWTLEDVLNENLYKDKVHKIPETFKSATDYKNSFIPLLFEETRADLSSSLSGVSQAALCEIKNVEHSKQLKLRKAQNQFIQFHHTIWLKSTTDYEPASGDLIAITYIRPKSLNDLNTLNSPYHIAYVNGEKNRFSGRITVLSSKCMKMDVESVSRKNNTQKMYAVYIMNMTTNVRIWKALDSKSKGDHLNIIEKVLQPNLNSGENCKICMSGSNSQASFITKDIIQSQNLNESQQDAVTSCVSMVNCSHANTKLIWGPPGTGKTKTVACLLFSLLKLKSRTLTCAPTNTAVLQVATRLHSLVTGSLDHDTYGLADIVLFGNGKRMKVDSYPGLEDIFLDYRVKNLMQCYAEWKHSFEAMIEFLSDPSKQYFLEMSKKDFGMDKNRILASAYHAYKINKGNYGLIMRFEDYVQKARTEITKLYQLDENDKKECMLTIEQFVKQRIDKLSMNRVNIFMTLYTSLMQLLEDPREQLFSKMGYKSLDDFATNSIVVSAYSAYKQNKGSDKYDNTLTFEGYVKRARKEIIELYQSIMTMEQFVKQRYLELREKLKFLLLTLYTHMPKSFISVNNILQALDSLKSLEISLRQAKFKQAVADCEEECIPVCLGPSSLERKDCLYILSFLSKSISLPDFKVRHLVEKFCLSNASLILCTVSSSIKLYSEEKSPVTFLVIDEAAMLKECESTIPLQLPGLCHCILIGDERQLPALVKSKIADECDFGRSMFERLVTSGYKRHMLNVQYRMHPSISLFPCKEFYDGKISDAVIVRKEKYNKHFLQGKMYASYSFINIAKGKEQFGCGNSLKNMVEVAVISKILESLKHEFMRTKKKVSIGIISPYNAQVFEIQEKVKQYIAVSDTDFSVSVRSVDGSQGGEEDIIIISTILPLDIRKCSYSNQQ*