AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0448680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0448680 MtrunA17_Chr1g0209681 96.296 81 3 0 15 95 56 136 5.70e-46 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0448680 AT1G57600.1 62.195 82 31 0 19 100 60 141 2.66e-30 112
Msa0448680 AT1G57600.2 62.195 82 31 0 19 100 60 141 2.66e-30 112
Msa0448680 AT1G57600.3 62.195 82 31 0 19 100 60 141 2.66e-30 112

Find 103 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAGGATCCTTCCTTTC+TGG 0.164123 3_3:+29735632 Msa0448680:three_prime_UTR
CAAGTAGATATTTATTTAAA+AGG 0.207136 3_3:+29735535 Msa0448680:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 3_3:+29733683 Msa0448680:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 3_3:+29734470 Msa0448680:three_prime_UTR
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 3_3:-29733318 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 3_3:+29733201 Msa0448680:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 3_3:+29733666 Msa0448680:CDS
GCTTAGTGTGAGATTAGTTT+TGG 0.293666 3_3:+29736043 Msa0448680:three_prime_UTR
TTCTTCTTCCTTCGCGAATT+CGG 0.299784 3_3:-29732648 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 3_3:-29735780 None:intergenic
ATAAAATTATAAGTCTAAAT+AGG 0.331713 3_3:-29735576 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 3_3:+29732580 Msa0448680:five_prime_UTR
AGGATCCTTCCTTTCTGGTT+AGG 0.351041 3_3:+29735637 Msa0448680:three_prime_UTR
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 3_3:+29735991 Msa0448680:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 3_3:+29736318 Msa0448680:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 3_3:+29734450 Msa0448680:exon
AATCTCACACTAAGCTGAAT+TGG 0.358285 3_3:-29736035 None:intergenic
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 3_3:-29733706 None:intergenic
GAGAGATCAAACTTCTTAGT+TGG 0.360705 3_3:-29733125 None:intergenic
GAAAAGGGAAAAGGGAAAAG+TGG 0.365827 3_3:-29732395 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 3_3:-29732631 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 3_3:-29732574 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 3_3:+29733202 Msa0448680:five_prime_UTR
TACAAAATCATAGAATTATA+AGG 0.389269 3_3:-29736254 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 3_3:-29732573 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 3_3:-29732403 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 3_3:-29733246 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 3_3:-29732404 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 3_3:-29732440 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 3_3:+29736337 Msa0448680:three_prime_UTR
TCAATTTAATGAGAAGTGAT+AGG 0.441583 3_3:+29735911 Msa0448680:three_prime_UTR
AGAGATCAAACTTCTTAGTT+GGG 0.445059 3_3:-29733124 None:intergenic
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 3_3:-29732411 None:intergenic
AAAAGGGAAAAGGGAAAAGT+GGG 0.469224 3_3:-29732394 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 3_3:+29733777 Msa0448680:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 3_3:-29732762 None:intergenic
ATGCTCCATGCAAATAAGAT+AGG 0.474131 3_3:-29733782 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 3_3:-29733106 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 3_3:+29733739 Msa0448680:CDS
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 3_3:-29732421 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 3_3:-29732486 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.492024 3_3:-29732445 None:intergenic
AAATGTAAGTAAAGGAGATA+GGG 0.493237 3_3:+29735992 Msa0448680:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 3_3:+29736066 Msa0448680:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 3_3:-29733666 None:intergenic
AGGAGCTAGGCTAAACCATC+AGG 0.501207 3_3:+29735931 Msa0448680:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 3_3:+29733164 Msa0448680:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 3_3:-29733317 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 3_3:-29735878 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 3_3:-29732559 None:intergenic
ACATACCGCGAGAAAGTTGA+AGG 0.522314 3_3:-29733391 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 3_3:+29733697 Msa0448680:CDS
TATTGATGAGAATATATGGC+AGG 0.526440 3_3:+29735596 Msa0448680:three_prime_UTR
CTCTTGCGATTCCACGATTG+GGG 0.530968 3_3:+29733203 Msa0448680:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 3_3:+29733723 Msa0448680:CDS
AAGAAATTCCACCTCCATCT+CGG 0.534799 3_3:+29735864 Msa0448680:three_prime_UTR
AAGAAAACGCATGTGAGCGT+TGG 0.536923 3_3:+29735490 Msa0448680:three_prime_UTR
TTCTCATTAAATTGACGAGA+TGG 0.542657 3_3:-29735903 None:intergenic
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 3_3:-29732420 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 3_3:-29732410 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 3_3:-29735872 None:intergenic
CTCTTAGCTTGGCAGTGCAT+AGG 0.552112 3_3:+29736212 Msa0448680:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 3_3:-29735946 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 3_3:+29733698 Msa0448680:CDS
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 3_3:-29732391 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 3_3:+29734454 Msa0448680:exon
ACAGTATCCGACAGCATCTG+AGG 0.568896 3_3:+29734437 Msa0448680:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 3_3:+29735918 Msa0448680:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 3_3:+29733784 Msa0448680:CDS
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 3_3:+29732727 Msa0448680:five_prime_UTR
AACAACCTAACCAGAAAGGA+AGG 0.580432 3_3:-29735642 None:intergenic
CGCTCCCTTCAACTTTCTCG+CGG 0.582697 3_3:+29733386 Msa0448680:CDS
TCATTAAATTGCCGAGATGG+AGG 0.583358 3_3:-29735875 None:intergenic
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 3_3:-29733197 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 3_3:+29733157 Msa0448680:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 3_3:+29735775 Msa0448680:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 3_3:-29733214 None:intergenic
CAGTATCCGACAGCATCTGA+GGG 0.590060 3_3:+29734438 Msa0448680:CDS
AGTTTCCCCTCAGATGCTGT+CGG 0.590938 3_3:-29734444 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 3_3:-29732419 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 3_3:+29735776 Msa0448680:three_prime_UTR
ATAAAACAACCTAACCAGAA+AGG 0.600525 3_3:-29735646 None:intergenic
CTTCTTAGTTGGGAGATCGA+AGG 0.601728 3_3:-29733114 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 3_3:+29733170 Msa0448680:five_prime_UTR
GGACTTTGCTAAGACTTGAG+AGG 0.614168 3_3:+29735617 Msa0448680:three_prime_UTR
ATAGGAGACTTGTGTTTCAG+CGG 0.616380 3_3:-29735558 None:intergenic
CTCCCATAGATTGTCTCAAG+GGG 0.617721 3_3:+29735777 Msa0448680:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 3_3:+29735732 Msa0448680:intron
ATCCCCTTGAGACAATCTAT+GGG 0.622438 3_3:-29735779 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 3_3:+29732702 Msa0448680:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 3_3:+29733625 Msa0448680:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 3_3:+29732640 Msa0448680:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 3_3:+29732622 Msa0448680:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 3_3:-29732390 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 3_3:-29732495 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 3_3:-29732501 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 3_3:+29733274 Msa0448680:five_prime_UTR
AGAAAACAAAAGCCAGACAA+CGG 0.663004 3_3:-29733751 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 3_3:-29733778 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 3_3:+29733300 Msa0448680:five_prime_UTR
AGGATGTCTCAGATGCTCAG+TGG 0.679281 3_3:+29733622 Msa0448680:intron
CATACCGCGAGAAAGTTGAA+GGG 0.694597 3_3:-29733390 None:intergenic
AGTATCCGACAGCATCTGAG+GGG 0.723113 3_3:+29734439 Msa0448680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAAATA+TGG - chr3_3:29734557-29734576 None:intergenic 10.0%
!! ATAAAATTATAAGTCTAAAT+AGG - chr3_3:29735579-29735598 None:intergenic 10.0%
!!! ATGAAAAGTGAATTTTTTTT+AGG - chr3_3:29732367-29732386 None:intergenic 15.0%
!!! ATTTGTTTTTTATTTATCGT+CGG + chr3_3:29733484-29733503 Msa0448680:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG + chr3_3:29733921-29733940 Msa0448680:CDS 15.0%
!! AAACAATGAAAATTAATCTT+TGG - chr3_3:29734285-29734304 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG + chr3_3:29734284-29734303 Msa0448680:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG - chr3_3:29734658-29734677 None:intergenic 15.0%
!! GATAATAGATGAATAAATAA+AGG + chr3_3:29735346-29735365 Msa0448680:intron 15.0%
!! CAAGTAGATATTTATTTAAA+AGG + chr3_3:29735535-29735554 Msa0448680:three_prime_UTR 15.0%
!!! ATTTTATTGATGAGAATATA+TGG + chr3_3:29735592-29735611 Msa0448680:three_prime_UTR 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG + chr3_3:29735711-29735730 Msa0448680:intron 15.0%
!! TACAAAATCATAGAATTATA+AGG - chr3_3:29736257-29736276 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG + chr3_3:29736309-29736328 Msa0448680:three_prime_UTR 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG - chr3_3:29732889-29732908 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG + chr3_3:29733979-29733998 Msa0448680:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG + chr3_3:29734078-29734097 Msa0448680:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG - chr3_3:29734366-29734385 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG + chr3_3:29734365-29734384 Msa0448680:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG + chr3_3:29734691-29734710 Msa0448680:intron 20.0%
!!! ATACAATTATTCATTTGTTG+AGG - chr3_3:29734941-29734960 None:intergenic 20.0%
!! ATATGAATAGTATATGATGT+TGG + chr3_3:29734974-29734993 Msa0448680:intron 20.0%
!! TATGAATAGTATATGATGTT+GGG + chr3_3:29734975-29734994 Msa0448680:intron 20.0%
!!! GTTCTTTAATTTTTACAAGT+TGG + chr3_3:29734997-29735016 Msa0448680:intron 20.0%
!! AAAAACAATTATTATGACAC+CGG - chr3_3:29735117-29735136 None:intergenic 20.0%
!!! TTTTTTTTGTTGTGTAATGT+AGG + chr3_3:29735432-29735451 Msa0448680:intron 20.0%
!! AAATAACACACAAATTCATT+AGG - chr3_3:29735707-29735726 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG + chr3_3:29735984-29736003 Msa0448680:three_prime_UTR 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG + chr3_3:29736140-29736159 Msa0448680:three_prime_UTR 20.0%
! AAAAATTGTTCGATTGAGAA+CGG - chr3_3:29732452-29732471 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG + chr3_3:29732796-29732815 Msa0448680:intron 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG + chr3_3:29732904-29732923 Msa0448680:intron 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG + chr3_3:29733070-29733089 Msa0448680:five_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG + chr3_3:29733363-29733382 Msa0448680:CDS 25.0%
! TTACACATAACAAACAGAAT+AGG - chr3_3:29733602-29733621 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG + chr3_3:29733602-29733621 Msa0448680:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG - chr3_3:29734181-29734200 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG - chr3_3:29734273-29734292 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG + chr3_3:29734397-29734416 Msa0448680:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG + chr3_3:29734523-29734542 Msa0448680:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG - chr3_3:29734551-29734570 None:intergenic 25.0%
! AGCATTATATATGCTCTAAT+TGG + chr3_3:29734906-29734925 Msa0448680:intron 25.0%
! TATTTGTGTTATATAGGTGT+CGG - chr3_3:29735172-29735191 None:intergenic 25.0%
! ATATGAAACTCTTGTATCTA+TGG - chr3_3:29735257-29735276 None:intergenic 25.0%
! TTGAGAAATGTTGATCAATT+AGG + chr3_3:29735280-29735299 Msa0448680:intron 25.0%
! TGAGAAATGTTGATCAATTA+GGG + chr3_3:29735281-29735300 Msa0448680:intron 25.0%
!!! TTTTTTACTATTTCAGCAAC+AGG - chr3_3:29735472-29735491 None:intergenic 25.0%
! TCAATTTAATGAGAAGTGAT+AGG + chr3_3:29735911-29735930 Msa0448680:three_prime_UTR 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG + chr3_3:29735991-29736010 Msa0448680:three_prime_UTR 25.0%
! AAATGTAAGTAAAGGAGATA+GGG + chr3_3:29735992-29736011 Msa0448680:three_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG - chr3_3:29736130-29736149 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG + chr3_3:29736161-29736180 Msa0448680:three_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG + chr3_3:29736318-29736337 Msa0448680:three_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG - chr3_3:29732741-29732760 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG + chr3_3:29732853-29732872 Msa0448680:intron 30.0%
CTCGTTGGATAAATTTACAT+TGG + chr3_3:29732969-29732988 Msa0448680:intron 30.0%
AGAGATCAAACTTCTTAGTT+GGG - chr3_3:29733127-29733146 None:intergenic 30.0%
CTACATAAACAGGTTATACA+TGG - chr3_3:29733433-29733452 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG - chr3_3:29733443-29733462 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG + chr3_3:29733512-29733531 Msa0448680:intron 30.0%
AATCATACATTGAGGTGATT+AGG - chr3_3:29733560-29733579 None:intergenic 30.0%
ATGACTAGAATCATACATTG+AGG - chr3_3:29733568-29733587 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG + chr3_3:29733723-29733742 Msa0448680:CDS 30.0%
TTATCTCTAACAGCAAGATA+AGG - chr3_3:29734203-29734222 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG - chr3_3:29734236-29734255 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG + chr3_3:29734316-29734335 Msa0448680:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG + chr3_3:29734470-29734489 Msa0448680:three_prime_UTR 30.0%
TTCGAGTGACAAATGAAAAT+AGG - chr3_3:29734505-29734524 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG - chr3_3:29734584-29734603 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG - chr3_3:29734594-29734613 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG + chr3_3:29734720-29734739 Msa0448680:intron 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG + chr3_3:29734721-29734740 Msa0448680:intron 30.0%
ATGCTAAATACACTTGAACA+CGG + chr3_3:29734771-29734790 Msa0448680:intron 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG + chr3_3:29734795-29734814 Msa0448680:intron 30.0%
CGGTTGTATTTGTGTTATAT+AGG - chr3_3:29735178-29735197 None:intergenic 30.0%
GAGAAATGTTGATCAATTAG+GGG + chr3_3:29735282-29735301 Msa0448680:intron 30.0%
TATTGATGAGAATATATGGC+AGG + chr3_3:29735596-29735615 Msa0448680:three_prime_UTR 30.0%
ATAAAACAACCTAACCAGAA+AGG - chr3_3:29735649-29735668 None:intergenic 30.0%
TTCTCATTAAATTGACGAGA+TGG - chr3_3:29735906-29735925 None:intergenic 30.0%
! TTTTAGTCTAACTCTTAGCT+TGG + chr3_3:29736201-29736220 Msa0448680:three_prime_UTR 30.0%
TTGGATATTGTAACAATGAC+AGG + chr3_3:29736337-29736356 Msa0448680:three_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG - chr3_3:29732397-29732416 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG - chr3_3:29732504-29732523 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG - chr3_3:29732562-29732581 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG + chr3_3:29733057-29733076 Msa0448680:intron 35.0%
GAGAGATCAAACTTCTTAGT+TGG - chr3_3:29733128-29733147 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG - chr3_3:29733249-29733268 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG + chr3_3:29733683-29733702 Msa0448680:CDS 35.0%
AGAAAACAAAAGCCAGACAA+CGG - chr3_3:29733754-29733773 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG - chr3_3:29733781-29733800 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG - chr3_3:29733785-29733804 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG + chr3_3:29733784-29733803 Msa0448680:CDS 35.0%
! ACACGGATTTTGCATTTTAG+TGG + chr3_3:29734788-29734807 Msa0448680:intron 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG + chr3_3:29734796-29734815 Msa0448680:intron 35.0%
TAACACAAATACAACCGTGT+CGG + chr3_3:29735181-29735200 Msa0448680:intron 35.0%
TTCTCATTAAATTGCCGAGA+TGG - chr3_3:29735881-29735900 None:intergenic 35.0%
AATCTCACACTAAGCTGAAT+TGG - chr3_3:29736038-29736057 None:intergenic 35.0%
!!! GCTTAGTGTGAGATTAGTTT+TGG + chr3_3:29736043-29736062 Msa0448680:three_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG + chr3_3:29736066-29736085 Msa0448680:three_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG + chr3_3:29736073-29736092 Msa0448680:three_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG + chr3_3:29736228-29736247 Msa0448680:three_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG - chr3_3:29732398-29732417 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG - chr3_3:29732448-29732467 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG - chr3_3:29732489-29732508 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG - chr3_3:29732498-29732517 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG + chr3_3:29732622-29732641 Msa0448680:five_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG - chr3_3:29732634-29732653 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG - chr3_3:29732651-29732670 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG + chr3_3:29732702-29732721 Msa0448680:five_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG + chr3_3:29732751-29732770 Msa0448680:five_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG - chr3_3:29732958-29732977 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG + chr3_3:29733048-29733067 Msa0448680:intron 40.0%
TCAACAACTGTACTCAAACC+TGG + chr3_3:29733157-29733176 Msa0448680:five_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG + chr3_3:29733164-29733183 Msa0448680:five_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG + chr3_3:29733274-29733293 Msa0448680:five_prime_UTR 40.0%
AAGAACTCTTTCTGCTTCCA+GGG - chr3_3:29733320-29733339 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG - chr3_3:29733321-29733340 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG - chr3_3:29733669-29733688 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG + chr3_3:29733666-29733685 Msa0448680:CDS 40.0%
ACTTCTGGCCAATTTGATGA+GGG + chr3_3:29733698-29733717 Msa0448680:CDS 40.0%
GAAGAAAGCCCTCATCAAAT+TGG - chr3_3:29733709-29733728 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG + chr3_3:29733777-29733796 Msa0448680:CDS 40.0%
TATTTGCATGGAGCATGGTA+TGG + chr3_3:29733789-29733808 Msa0448680:intron 40.0%
TTAAATATGGCACAGGAACC+AGG - chr3_3:29734544-29734563 None:intergenic 40.0%
CGTTCCTTATCTATGAAGCA+CGG + chr3_3:29735025-29735044 Msa0448680:intron 40.0%
GTATCCGTGCTTCATAGATA+AGG - chr3_3:29735032-29735051 None:intergenic 40.0%
TACTTCGACACTCTTACGAT+CGG + chr3_3:29735143-29735162 Msa0448680:intron 40.0%
ATAGGAGACTTGTGTTTCAG+CGG - chr3_3:29735561-29735580 None:intergenic 40.0%
AACAACCTAACCAGAAAGGA+AGG - chr3_3:29735645-29735664 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG + chr3_3:29735775-29735794 Msa0448680:three_prime_UTR 40.0%
ACTCCCATAGATTGTCTCAA+GGG + chr3_3:29735776-29735795 Msa0448680:three_prime_UTR 40.0%
ATCCCCTTGAGACAATCTAT+GGG - chr3_3:29735782-29735801 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG - chr3_3:29735783-29735802 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG + chr3_3:29735864-29735883 Msa0448680:three_prime_UTR 40.0%
TCATTAAATTGCCGAGATGG+AGG - chr3_3:29735878-29735897 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG + chr3_3:29735918-29735937 Msa0448680:three_prime_UTR 40.0%
TCTGATGTATAAGCTCCTGA+TGG - chr3_3:29735949-29735968 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG - chr3_3:29732406-29732425 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG - chr3_3:29732407-29732426 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG - chr3_3:29732423-29732442 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG - chr3_3:29732424-29732443 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG - chr3_3:29732443-29732462 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG - chr3_3:29732576-29732595 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG - chr3_3:29732577-29732596 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG + chr3_3:29732580-29732599 Msa0448680:five_prime_UTR 45.0%
AAAGGTAACCGAATTCGCGA+AGG + chr3_3:29732640-29732659 Msa0448680:five_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG - chr3_3:29732765-29732784 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG - chr3_3:29733034-29733053 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG - chr3_3:29733046-29733065 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG + chr3_3:29733047-29733066 Msa0448680:intron 45.0%
CTTCTTAGTTGGGAGATCGA+AGG - chr3_3:29733117-29733136 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG + chr3_3:29733202-29733221 Msa0448680:five_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG + chr3_3:29733300-29733319 Msa0448680:five_prime_UTR 45.0%
CATACCGCGAGAAAGTTGAA+GGG - chr3_3:29733393-29733412 None:intergenic 45.0%
ACATACCGCGAGAAAGTTGA+AGG - chr3_3:29733394-29733413 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG + chr3_3:29733523-29733542 Msa0448680:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG - chr3_3:29733545-29733564 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG + chr3_3:29733697-29733716 Msa0448680:CDS 45.0%
GCATCTGAGGGGAAACTAAA+TGG + chr3_3:29734450-29734469 Msa0448680:exon 45.0%
CTGAGGGGAAACTAAATGGT+TGG + chr3_3:29734454-29734473 Msa0448680:exon 45.0%
TATGAAGCACGGATACCTCT+AGG + chr3_3:29735036-29735055 Msa0448680:intron 45.0%
TTTGTCTGAGTTGTCCGACA+CGG - chr3_3:29735198-29735217 None:intergenic 45.0%
ACTCTTGTATCTATGGTGCG+CGG - chr3_3:29735250-29735269 None:intergenic 45.0%
AAGAAAACGCATGTGAGCGT+TGG + chr3_3:29735490-29735509 Msa0448680:three_prime_UTR 45.0%
GGACTTTGCTAAGACTTGAG+AGG + chr3_3:29735617-29735636 Msa0448680:three_prime_UTR 45.0%
TTGAGAGGATCCTTCCTTTC+TGG + chr3_3:29735632-29735651 Msa0448680:three_prime_UTR 45.0%
!! AGGATCCTTCCTTTCTGGTT+AGG + chr3_3:29735637-29735656 Msa0448680:three_prime_UTR 45.0%
GGCGAAAAGAACGAAGACAT+AGG + chr3_3:29735732-29735751 Msa0448680:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG + chr3_3:29735777-29735796 Msa0448680:three_prime_UTR 45.0%
TTAAATTGCCGAGATGGAGG+TGG - chr3_3:29735875-29735894 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG + chr3_3:29736079-29736098 Msa0448680:three_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG + chr3_3:29736082-29736101 Msa0448680:three_prime_UTR 45.0%
!!! GGTTTTTGGAGTAGGTGGTT+CGG + chr3_3:29736087-29736106 Msa0448680:three_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG - chr3_3:29732393-29732412 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG - chr3_3:29732394-29732413 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG - chr3_3:29732413-29732432 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG - chr3_3:29732414-29732433 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG - chr3_3:29732422-29732441 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG + chr3_3:29732762-29732781 Msa0448680:five_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG + chr3_3:29732909-29732928 Msa0448680:intron 50.0%
GCCGGAATAGCAAATCTCGT+TGG + chr3_3:29732954-29732973 Msa0448680:intron 50.0%
TTGGGAGATCGAAGGAAGAC+AGG - chr3_3:29733109-29733128 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG + chr3_3:29733170-29733189 Msa0448680:five_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG - chr3_3:29733200-29733219 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG + chr3_3:29733201-29733220 Msa0448680:five_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG + chr3_3:29733203-29733222 Msa0448680:five_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG + chr3_3:29733524-29733543 Msa0448680:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG - chr3_3:29733544-29733563 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG + chr3_3:29733622-29733641 Msa0448680:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG + chr3_3:29733625-29733644 Msa0448680:CDS 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG + chr3_3:29733636-29733655 Msa0448680:CDS 50.0%
TGAGAGGAATGTCCGTTGTC+TGG + chr3_3:29733739-29733758 Msa0448680:CDS 50.0%
ACAGTATCCGACAGCATCTG+AGG + chr3_3:29734437-29734456 Msa0448680:CDS 50.0%
CAGTATCCGACAGCATCTGA+GGG + chr3_3:29734438-29734457 Msa0448680:CDS 50.0%
AGTATCCGACAGCATCTGAG+GGG + chr3_3:29734439-29734458 Msa0448680:CDS 50.0%
AGTTTCCCCTCAGATGCTGT+CGG - chr3_3:29734447-29734466 None:intergenic 50.0%
! CCGGTTCGACACATATCGTA+CGG - chr3_3:29735098-29735117 None:intergenic 50.0%
CCGTACGATATGTGTCGAAC+CGG + chr3_3:29735095-29735114 Msa0448680:intron 50.0%
AGGAGCTAGGCTAAACCATC+AGG + chr3_3:29735931-29735950 Msa0448680:three_prime_UTR 50.0%
! CTCTTAGCTTGGCAGTGCAT+AGG + chr3_3:29736212-29736231 Msa0448680:three_prime_UTR 50.0%
GCAGCTCGAATCCGAAGAAG+AGG + chr3_3:29732727-29732746 Msa0448680:five_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG - chr3_3:29732950-29732969 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG - chr3_3:29732953-29732972 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG - chr3_3:29733217-29733236 None:intergenic 55.0%
CGCTCCCTTCAACTTTCTCG+CGG + chr3_3:29733386-29733405 Msa0448680:CDS 55.0%
TATTCGGTTTACGCCCGGGA+TGG + chr3_3:29733528-29733547 Msa0448680:intron 55.0%
!! GCACGGATACCTCTAGGACT+AGG + chr3_3:29735042-29735061 Msa0448680:intron 55.0%
!! CTCTAGGACTAGGCATGTCG+CGG + chr3_3:29735052-29735071 Msa0448680:intron 55.0%
!! GTACGGTGTCATACGCGTGT+CGG - chr3_3:29735081-29735100 None:intergenic 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG + chr3_3:29732912-29732931 Msa0448680:intron 60.0%
! GAACACCGCGTCCGTTTTCC+AGG - chr3_3:29733178-29733197 None:intergenic 60.0%
CGCGACATGCCTAGTCCTAG+AGG - chr3_3:29735054-29735073 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG - chr3_3:29732946-29732965 None:intergenic 65.0%
TATTCCGGCGAGGTGGCCGG+TGG - chr3_3:29732943-29732962 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG - chr3_3:29732926-29732945 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG + chr3_3:29732924-29732943 Msa0448680:intron 80.0%
! GCCACCACCGGCCACCTCGC+CGG + chr3_3:29732936-29732955 Msa0448680:intron 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG - chr3_3:29732940-29732959 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 29732354 29736404 29732354 ID=Msa0448680;Name=Msa0448680
chr3_3 mRNA 29732354 29736404 29732354 ID=Msa0448680-mRNA-1;Parent=Msa0448680;Name=Msa0448680-mRNA-1;_AED=0.21;_eAED=0.21;_QI=718|1|1|1|0.16|0.42|7|900|108
chr3_3 exon 29732354 29732783 29732354 ID=Msa0448680-mRNA-1:exon:5595;Parent=Msa0448680-mRNA-1
chr3_3 exon 29733066 29733407 29733066 ID=Msa0448680-mRNA-1:exon:5596;Parent=Msa0448680-mRNA-1
chr3_3 exon 29733624 29733805 29733624 ID=Msa0448680-mRNA-1:exon:5597;Parent=Msa0448680-mRNA-1
chr3_3 exon 29733894 29733942 29733894 ID=Msa0448680-mRNA-1:exon:5598;Parent=Msa0448680-mRNA-1
chr3_3 exon 29734427 29734491 29734427 ID=Msa0448680-mRNA-1:exon:5599;Parent=Msa0448680-mRNA-1
chr3_3 exon 29735454 29735658 29735454 ID=Msa0448680-mRNA-1:exon:5600;Parent=Msa0448680-mRNA-1
chr3_3 exon 29735733 29736404 29735733 ID=Msa0448680-mRNA-1:exon:5601;Parent=Msa0448680-mRNA-1
chr3_3 five_prime_UTR 29732354 29732783 29732354 ID=Msa0448680-mRNA-1:five_prime_utr;Parent=Msa0448680-mRNA-1
chr3_3 five_prime_UTR 29733066 29733353 29733066 ID=Msa0448680-mRNA-1:five_prime_utr;Parent=Msa0448680-mRNA-1
chr3_3 CDS 29733354 29733407 29733354 ID=Msa0448680-mRNA-1:cds;Parent=Msa0448680-mRNA-1
chr3_3 CDS 29733624 29733805 29733624 ID=Msa0448680-mRNA-1:cds;Parent=Msa0448680-mRNA-1
chr3_3 CDS 29733894 29733942 29733894 ID=Msa0448680-mRNA-1:cds;Parent=Msa0448680-mRNA-1
chr3_3 CDS 29734427 29734468 29734427 ID=Msa0448680-mRNA-1:cds;Parent=Msa0448680-mRNA-1
chr3_3 three_prime_UTR 29734469 29734491 29734469 ID=Msa0448680-mRNA-1:three_prime_utr;Parent=Msa0448680-mRNA-1
chr3_3 three_prime_UTR 29735454 29735658 29735454 ID=Msa0448680-mRNA-1:three_prime_utr;Parent=Msa0448680-mRNA-1
chr3_3 three_prime_UTR 29735733 29736404 29735733 ID=Msa0448680-mRNA-1:three_prime_utr;Parent=Msa0448680-mRNA-1
Gene Sequence

>Msa0448680

ATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTTCAACTTTCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCCGACAGCATCTGAGGGGAAACTAA
Protein sequence

>Msa0448680

MHSFSMLSSSGAPFNFLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSIRQHLRGN*