Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0739350 | KEH33596.1 | 91.803 | 61 | 5 | 0 | 1 | 61 | 53 | 113 | 6.92e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0739350 | G7ZWU3 | 91.803 | 61 | 5 | 0 | 1 | 61 | 53 | 113 | 3.30e-33 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0739350 | Msa0777190 | 0.844208 | 8.715034e-59 | -8.615850e-47 |
| Msa0739350 | Msa1015450 | 0.846190 | 2.537106e-59 | -8.615850e-47 |
| Msa0739350 | Msa1133620 | 0.805136 | 1.544326e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0739350 | MtrunA17_Chr8g0356411 | 93.443 | 61 | 4 | 0 | 1 | 61 | 1 | 61 | 9.62e-37 | 117 |
| Msa0739350 | MtrunA17_Chr5g0419101 | 89.655 | 58 | 6 | 0 | 1 | 58 | 45 | 102 | 3.85e-33 | 109 |
| Msa0739350 | MtrunA17_Chr2g0309021 | 87.931 | 58 | 7 | 0 | 1 | 58 | 469 | 526 | 4.01e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGTATCATTGGGAAATA+AGG | 0.351637 | 5_1:+57492623 | None:intergenic |
| TTATACCTGTTGTGTATCAT+TGG | 0.404371 | 5_1:+57492614 | None:intergenic |
| GATGATCTAGATATGTCATT+AGG | 0.423580 | 5_1:+57491943 | None:intergenic |
| TGCAACTATGGAGGCTCAAA+TGG | 0.437214 | 5_1:-57492709 | None:intergenic |
| GCTCAAATGGCAGCACTTGT+TGG | 0.439947 | 5_1:-57492696 | Msa0739350:CDS |
| ATTTCCCAATGATACACAAC+AGG | 0.483592 | 5_1:-57492619 | Msa0739350:intron |
| AATAAGGCAGCAGCTAACTC+TGG | 0.498255 | 5_1:+57492639 | None:intergenic |
| TATACCTGTTGTGTATCATT+GGG | 0.541600 | 5_1:+57492615 | None:intergenic |
| CTAGATATGTCATTAGGTGT+TGG | 0.547570 | 5_1:+57491949 | None:intergenic |
| TACATTAAAGCGAAGGAAGG+TGG | 0.589028 | 5_1:-57492672 | Msa0739350:CDS |
| TGTTGGCTACATTAAAGCGA+AGG | 0.611559 | 5_1:-57492679 | Msa0739350:CDS |
| GGCTACATTAAAGCGAAGGA+AGG | 0.617737 | 5_1:-57492675 | Msa0739350:CDS |
| TCAGATGAAAGCAACGCACG+TGG | 0.667433 | 5_1:-57491922 | Msa0739350:CDS |
| AAGGCAGCAGCTAACTCTGG+AGG | 0.673734 | 5_1:+57492642 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr5_1:57492253-57492272 | Msa0739350:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr5_1:57492373-57492392 | Msa0739350:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr5_1:57492497-57492516 | Msa0739350:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr5_1:57492613-57492632 | Msa0739350:intron | 15.0% |
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr5_1:57492253-57492272 | Msa0739350:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr5_1:57492373-57492392 | Msa0739350:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr5_1:57492497-57492516 | Msa0739350:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr5_1:57492613-57492632 | Msa0739350:intron | 15.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr5_1:57492136-57492155 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr5_1:57492137-57492156 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr5_1:57492252-57492271 | Msa0739350:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr5_1:57492286-57492305 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr5_1:57492550-57492569 | Msa0739350:intron | 20.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr5_1:57492136-57492155 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr5_1:57492137-57492156 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr5_1:57492252-57492271 | Msa0739350:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr5_1:57492286-57492305 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr5_1:57492550-57492569 | Msa0739350:intron | 20.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr5_1:57492079-57492098 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr5_1:57492083-57492102 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr5_1:57492259-57492278 | Msa0739350:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr5_1:57492389-57492408 | Msa0739350:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr5_1:57492390-57492409 | Msa0739350:intron | 25.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr5_1:57492079-57492098 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr5_1:57492083-57492102 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr5_1:57492259-57492278 | Msa0739350:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr5_1:57492389-57492408 | Msa0739350:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr5_1:57492390-57492409 | Msa0739350:intron | 25.0% |
| TTGTGTATCATTGGGAAATA+AGG | + | chr5_1:57491988-57492007 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr5_1:57491996-57492015 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr5_1:57491997-57492016 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr5_1:57492128-57492147 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr5_1:57492163-57492182 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr5_1:57492200-57492219 | Msa0739350:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr5_1:57492668-57492687 | None:intergenic | 30.0% | |
| TTGTGTATCATTGGGAAATA+AGG | + | chr5_1:57491988-57492007 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr5_1:57491996-57492015 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr5_1:57491997-57492016 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr5_1:57492128-57492147 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr5_1:57492163-57492182 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr5_1:57492200-57492219 | Msa0739350:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr5_1:57492668-57492687 | None:intergenic | 30.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr5_1:57491989-57492008 | Msa0739350:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr5_1:57492038-57492057 | Msa0739350:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr5_1:57492318-57492337 | Msa0739350:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr5_1:57492562-57492581 | Msa0739350:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr5_1:57492662-57492681 | None:intergenic | 35.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr5_1:57491989-57492008 | Msa0739350:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr5_1:57492038-57492057 | Msa0739350:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr5_1:57492318-57492337 | Msa0739350:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr5_1:57492562-57492581 | Msa0739350:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr5_1:57492662-57492681 | None:intergenic | 35.0% | |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr5_1:57491929-57491948 | Msa0739350:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr5_1:57491936-57491955 | Msa0739350:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr5_1:57492072-57492091 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr5_1:57492110-57492129 | Msa0739350:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr5_1:57492626-57492645 | Msa0739350:CDS | 40.0% |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr5_1:57491929-57491948 | Msa0739350:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr5_1:57491936-57491955 | Msa0739350:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr5_1:57492072-57492091 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr5_1:57492110-57492129 | Msa0739350:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr5_1:57492626-57492645 | Msa0739350:CDS | 40.0% |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr5_1:57491933-57491952 | Msa0739350:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr5_1:57491972-57491991 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr5_1:57492185-57492204 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr5_1:57492331-57492350 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr5_1:57492340-57492359 | None:intergenic | 45.0% | |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr5_1:57491933-57491952 | Msa0739350:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr5_1:57491972-57491991 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr5_1:57492185-57492204 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr5_1:57492331-57492350 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr5_1:57492340-57492359 | None:intergenic | 45.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr5_1:57491912-57491931 | Msa0739350:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr5_1:57492336-57492355 | Msa0739350:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | - | chr5_1:57492686-57492705 | Msa0739350:CDS | 50.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr5_1:57491912-57491931 | Msa0739350:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr5_1:57492336-57492355 | Msa0739350:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | - | chr5_1:57492686-57492705 | Msa0739350:CDS | 50.0% | |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr5_1:57491969-57491988 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr5_1:57492325-57492344 | None:intergenic | 55.0% |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr5_1:57491969-57491988 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr5_1:57492325-57492344 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 57491906 | 57492724 | 57491906 | ID=Msa0739350;Name=Msa0739350 |
| chr5_1 | mRNA | 57491906 | 57492724 | 57491906 | ID=Msa0739350-mRNA-1;Parent=Msa0739350;Name=Msa0739350-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|0.5|2|0|64 |
| chr5_1 | exon | 57492620 | 57492724 | 57492620 | ID=Msa0739350-mRNA-1:exon:16379;Parent=Msa0739350-mRNA-1 |
| chr5_1 | exon | 57491906 | 57491995 | 57491906 | ID=Msa0739350-mRNA-1:exon:16378;Parent=Msa0739350-mRNA-1 |
| chr5_1 | CDS | 57492620 | 57492724 | 57492620 | ID=Msa0739350-mRNA-1:cds;Parent=Msa0739350-mRNA-1 |
| chr5_1 | CDS | 57491906 | 57491995 | 57491906 | ID=Msa0739350-mRNA-1:cds;Parent=Msa0739350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
