Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133620 | KEH33596.1 | 91.803 | 61 | 5 | 0 | 1 | 61 | 53 | 113 | 6.92e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133620 | G7ZWU3 | 91.803 | 61 | 5 | 0 | 1 | 61 | 53 | 113 | 3.30e-33 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0715410 | Msa1133620 | 0.823473 | 1.359542e-53 | -8.615850e-47 |
| Msa0739350 | Msa1133620 | 0.805136 | 1.544326e-49 | -8.615850e-47 |
| Msa0777190 | Msa1133620 | 0.871240 | 7.944164e-67 | -8.615850e-47 |
| Msa1015450 | Msa1133620 | 0.801995 | 6.921110e-49 | -8.615850e-47 |
| Msa1052330 | Msa1133620 | 0.801309 | 9.570047e-49 | -8.615850e-47 |
| Msa1133620 | Msa1308620 | 0.804822 | 1.796499e-49 | -8.615850e-47 |
| Msa0484780 | Msa1133620 | 0.803438 | 3.486354e-49 | -8.615850e-47 |
| Msa0523940 | Msa1133620 | 0.802035 | 6.792151e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133620 | MtrunA17_Chr8g0356411 | 93.443 | 61 | 4 | 0 | 1 | 61 | 1 | 61 | 9.62e-37 | 117 |
| Msa1133620 | MtrunA17_Chr5g0419101 | 89.655 | 58 | 6 | 0 | 1 | 58 | 45 | 102 | 3.85e-33 | 109 |
| Msa1133620 | MtrunA17_Chr2g0309021 | 87.931 | 58 | 7 | 0 | 1 | 58 | 469 | 526 | 4.01e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGTATCATTGGGAAATA+AGG | 0.351637 | 7_3:-87246586 | None:intergenic |
| TTATACCTGTTGTGTATCAT+TGG | 0.404371 | 7_3:-87246595 | None:intergenic |
| GATGATCTAGATATGTCATT+AGG | 0.423580 | 7_3:-87247266 | None:intergenic |
| TGCAACTATGGAGGCTCAAA+TGG | 0.437214 | 7_3:+87246500 | None:intergenic |
| GCTCAAATGGCAGCACTTGT+TGG | 0.439947 | 7_3:+87246513 | Msa1133620:CDS |
| ATTTCCCAATGATACACAAC+AGG | 0.483592 | 7_3:+87246590 | Msa1133620:CDS |
| AATAAGGCAGCAGCTAACTC+TGG | 0.498255 | 7_3:-87246570 | None:intergenic |
| TATACCTGTTGTGTATCATT+GGG | 0.541600 | 7_3:-87246594 | None:intergenic |
| CTAGATATGTCATTAGGTGT+TGG | 0.547570 | 7_3:-87247260 | None:intergenic |
| TACATTAAAGCGAAGGAAGG+TGG | 0.589028 | 7_3:+87246537 | Msa1133620:CDS |
| TGTTGGCTACATTAAAGCGA+AGG | 0.611559 | 7_3:+87246530 | Msa1133620:CDS |
| GGCTACATTAAAGCGAAGGA+AGG | 0.617737 | 7_3:+87246534 | Msa1133620:CDS |
| TCAGATGAAAGCAACGCACG+TGG | 0.667433 | 7_3:+87247287 | Msa1133620:CDS |
| AAGGCAGCAGCTAACTCTGG+AGG | 0.673734 | 7_3:-87246567 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATAAATGAAAGTTTTAA+GGG | + | chr7_3:87246854-87246873 | Msa1133620:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | + | chr7_3:87246974-87246993 | Msa1133620:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | + | chr7_3:87247098-87247117 | Msa1133620:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | + | chr7_3:87247214-87247233 | Msa1133620:intron | 15.0% |
| !!! | AGAATAAATGAAAGTTTTAA+GGG | + | chr7_3:87246854-87246873 | Msa1133620:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | + | chr7_3:87246974-87246993 | Msa1133620:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | + | chr7_3:87247098-87247117 | Msa1133620:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | + | chr7_3:87247214-87247233 | Msa1133620:intron | 15.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | - | chr7_3:87246737-87246756 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | - | chr7_3:87246738-87246757 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | + | chr7_3:87246853-87246872 | Msa1133620:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | - | chr7_3:87246887-87246906 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | + | chr7_3:87247151-87247170 | Msa1133620:intron | 20.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | - | chr7_3:87246737-87246756 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | - | chr7_3:87246738-87246757 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | + | chr7_3:87246853-87246872 | Msa1133620:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | - | chr7_3:87246887-87246906 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | + | chr7_3:87247151-87247170 | Msa1133620:intron | 20.0% |
| ! | TATAAATTTAGCAATGAGGA+AGG | - | chr7_3:87246680-87246699 | None:intergenic | 25.0% |
| ! | TCTCTATAAATTTAGCAATG+AGG | - | chr7_3:87246684-87246703 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | + | chr7_3:87246860-87246879 | Msa1133620:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | + | chr7_3:87246990-87247009 | Msa1133620:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | + | chr7_3:87246991-87247010 | Msa1133620:intron | 25.0% |
| ! | TATAAATTTAGCAATGAGGA+AGG | - | chr7_3:87246680-87246699 | None:intergenic | 25.0% |
| ! | TCTCTATAAATTTAGCAATG+AGG | - | chr7_3:87246684-87246703 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | + | chr7_3:87246860-87246879 | Msa1133620:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | + | chr7_3:87246990-87247009 | Msa1133620:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | + | chr7_3:87246991-87247010 | Msa1133620:intron | 25.0% |
| TTGTGTATCATTGGGAAATA+AGG | - | chr7_3:87246589-87246608 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | - | chr7_3:87246597-87246616 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | - | chr7_3:87246598-87246617 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | - | chr7_3:87246729-87246748 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | - | chr7_3:87246764-87246783 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | + | chr7_3:87246801-87246820 | Msa1133620:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | - | chr7_3:87247269-87247288 | None:intergenic | 30.0% | |
| TTGTGTATCATTGGGAAATA+AGG | - | chr7_3:87246589-87246608 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | - | chr7_3:87246597-87246616 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | - | chr7_3:87246598-87246617 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | - | chr7_3:87246729-87246748 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | - | chr7_3:87246764-87246783 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | + | chr7_3:87246801-87246820 | Msa1133620:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | - | chr7_3:87247269-87247288 | None:intergenic | 30.0% | |
| ATTTCCCAATGATACACAAC+AGG | + | chr7_3:87246590-87246609 | Msa1133620:CDS | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | + | chr7_3:87246639-87246658 | Msa1133620:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | + | chr7_3:87246919-87246938 | Msa1133620:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | + | chr7_3:87247163-87247182 | Msa1133620:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | - | chr7_3:87247263-87247282 | None:intergenic | 35.0% | |
| ATTTCCCAATGATACACAAC+AGG | + | chr7_3:87246590-87246609 | Msa1133620:CDS | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | + | chr7_3:87246639-87246658 | Msa1133620:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | + | chr7_3:87246919-87246938 | Msa1133620:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | + | chr7_3:87247163-87247182 | Msa1133620:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | - | chr7_3:87247263-87247282 | None:intergenic | 35.0% | |
| ! | TGTTGGCTACATTAAAGCGA+AGG | + | chr7_3:87246530-87246549 | Msa1133620:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | + | chr7_3:87246537-87246556 | Msa1133620:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | - | chr7_3:87246673-87246692 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | + | chr7_3:87246711-87246730 | Msa1133620:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | + | chr7_3:87247227-87247246 | Msa1133620:intron | 40.0% |
| ! | TGTTGGCTACATTAAAGCGA+AGG | + | chr7_3:87246530-87246549 | Msa1133620:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | + | chr7_3:87246537-87246556 | Msa1133620:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | - | chr7_3:87246673-87246692 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | + | chr7_3:87246711-87246730 | Msa1133620:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | + | chr7_3:87247227-87247246 | Msa1133620:intron | 40.0% |
| GGCTACATTAAAGCGAAGGA+AGG | + | chr7_3:87246534-87246553 | Msa1133620:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | - | chr7_3:87246573-87246592 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | - | chr7_3:87246786-87246805 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | - | chr7_3:87246932-87246951 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | - | chr7_3:87246941-87246960 | None:intergenic | 45.0% | |
| GGCTACATTAAAGCGAAGGA+AGG | + | chr7_3:87246534-87246553 | Msa1133620:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | - | chr7_3:87246573-87246592 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | - | chr7_3:87246786-87246805 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | - | chr7_3:87246932-87246951 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | - | chr7_3:87246941-87246960 | None:intergenic | 45.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | + | chr7_3:87246513-87246532 | Msa1133620:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | + | chr7_3:87246937-87246956 | Msa1133620:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | + | chr7_3:87247287-87247306 | Msa1133620:CDS | 50.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | + | chr7_3:87246513-87246532 | Msa1133620:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | + | chr7_3:87246937-87246956 | Msa1133620:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | + | chr7_3:87247287-87247306 | Msa1133620:CDS | 50.0% | |
| AAGGCAGCAGCTAACTCTGG+AGG | - | chr7_3:87246570-87246589 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | - | chr7_3:87246926-87246945 | None:intergenic | 55.0% |
| AAGGCAGCAGCTAACTCTGG+AGG | - | chr7_3:87246570-87246589 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | - | chr7_3:87246926-87246945 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 87246507 | 87247325 | 87246507 | ID=Msa1133620;Name=Msa1133620 |
| chr7_3 | mRNA | 87246507 | 87247325 | 87246507 | ID=Msa1133620-mRNA-1;Parent=Msa1133620;Name=Msa1133620-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|2|0|64 |
| chr7_3 | exon | 87246507 | 87246611 | 87246507 | ID=Msa1133620-mRNA-1:exon:26224;Parent=Msa1133620-mRNA-1 |
| chr7_3 | exon | 87247236 | 87247325 | 87247236 | ID=Msa1133620-mRNA-1:exon:26225;Parent=Msa1133620-mRNA-1 |
| chr7_3 | CDS | 87246507 | 87246611 | 87246507 | ID=Msa1133620-mRNA-1:cds;Parent=Msa1133620-mRNA-1 |
| chr7_3 | CDS | 87247236 | 87247325 | 87247236 | ID=Msa1133620-mRNA-1:cds;Parent=Msa1133620-mRNA-1 |
| Gene Sequence |
| Protein sequence |
