Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748200 | A2Q4I8 | 88.043 | 92 | 2 | 3 | 1 | 90 | 1 | 85 | 6.03e-32 | 116 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0748200 | Msa1160810 | 0.910382 | 1.995157e-82 | -8.615850e-47 |
| Msa0748200 | Msa1297230 | 0.807137 | 5.853683e-50 | -8.615850e-47 |
| Msa0748200 | Msa1314650 | 0.801535 | 8.600170e-49 | -8.615850e-47 |
| Msa0748200 | Msa1343430 | 0.802520 | 5.397184e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748200 | MtrunA17_Chr7g0255921 | 88.043 | 92 | 2 | 3 | 1 | 90 | 1 | 85 | 1.16e-35 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTGTTGTGTAGAGGGTT+TGG | 0.382242 | 5_2:+1345783 | None:intergenic |
| TGTGAAGCTGCAGCATTAAC+TGG | 0.394734 | 5_2:-1345936 | Msa0748200:CDS |
| CAACTTATCTGTTGTGTAGA+GGG | 0.487854 | 5_2:+1345778 | None:intergenic |
| GCAAGATTTGACAGATTCAA+AGG | 0.508269 | 5_2:-1345906 | Msa0748200:CDS |
| TCATCATCATCTAGATTCCA+AGG | 0.519300 | 5_2:-1345870 | Msa0748200:CDS |
| GTTAATGCTGCAGCTTCACA+TGG | 0.578700 | 5_2:+1345939 | None:intergenic |
| ACAACTTATCTGTTGTGTAG+AGG | 0.579808 | 5_2:+1345777 | None:intergenic |
| GAAAAGAGGAAAGTCTACAC+TGG | 0.638550 | 5_2:-1345807 | Msa0748200:CDS |
| CATCATCATCTAGATTCCAA+GGG | 0.644307 | 5_2:-1345869 | Msa0748200:CDS |
| AGATTTGACAGATTCAAAGG+TGG | 0.646343 | 5_2:-1345903 | Msa0748200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATAACAAAACATAGTTTT+TGG | + | chr5_2:1345808-1345827 | None:intergenic | 20.0% |
| !! | AAAAATGGAGATCAAGTTTT+TGG | - | chr5_2:1345980-1345999 | Msa0748200:CDS | 25.0% |
| !! | TTTTGTTTGCATTATTCCCT+TGG | + | chr5_2:1345964-1345983 | None:intergenic | 30.0% |
| GGGAATAATGCAAACAAAAA+TGG | - | chr5_2:1345965-1345984 | Msa0748200:CDS | 30.0% | |
| !!! | AAGTTTTTGGTGATGAAAAG+AGG | - | chr5_2:1345993-1346012 | Msa0748200:CDS | 30.0% |
| GCAAGATTTGACAGATTCAA+AGG | - | chr5_2:1345908-1345927 | Msa0748200:CDS | 35.0% | |
| AGATTTGACAGATTCAAAGG+TGG | - | chr5_2:1345911-1345930 | Msa0748200:CDS | 35.0% | |
| TCATCATCATCTAGATTCCA+AGG | - | chr5_2:1345944-1345963 | Msa0748200:CDS | 35.0% | |
| CATCATCATCTAGATTCCAA+GGG | - | chr5_2:1345945-1345964 | Msa0748200:CDS | 35.0% | |
| GAAAAGAGGAAAGTCTACAC+TGG | - | chr5_2:1346007-1346026 | Msa0748200:CDS | 40.0% | |
| TATCTGTTGTGTAGAGGGTT+TGG | + | chr5_2:1346034-1346053 | None:intergenic | 40.0% | |
| GTTAATGCTGCAGCTTCACA+TGG | + | chr5_2:1345878-1345897 | None:intergenic | 45.0% | |
| TGTGAAGCTGCAGCATTAAC+TGG | - | chr5_2:1345878-1345897 | Msa0748200:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 1345782 | 1346054 | 1345782 | ID=Msa0748200;Name=Msa0748200 |
| chr5_2 | mRNA | 1345782 | 1346054 | 1345782 | ID=Msa0748200-mRNA-1;Parent=Msa0748200;Name=Msa0748200-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|90 |
| chr5_2 | exon | 1345782 | 1346054 | 1345782 | ID=Msa0748200-mRNA-1:exon:356;Parent=Msa0748200-mRNA-1 |
| chr5_2 | CDS | 1345782 | 1346054 | 1345782 | ID=Msa0748200-mRNA-1:cds;Parent=Msa0748200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
