AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297230 MtrunA17_Chr4g0054351 97.269 659 12 3 1 654 1 658 0.0 1335
Msa1297230 MtrunA17_Chr3g0135161 53.198 688 256 23 1 653 1 657 0.0 566
Msa1297230 MtrunA17_Chr1g0152161 51.458 686 275 21 1 654 1 660 0.0 543
Msa1297230 MtrunA17_Chr5g0400871 48.099 684 185 29 1 654 1 544 1.47e-166 488
Msa1297230 MtrunA17_Chr8g0343851 47.251 491 181 10 18 492 3 431 1.00e-128 391
Msa1297230 MtrunA17_Chr4g0032631 57.862 318 105 7 248 558 121 416 1.00e-111 347
Msa1297230 MtrunA17_Chr4g0072291 72.018 218 58 1 293 507 143 360 5.66e-111 343
Msa1297230 MtrunA17_Chr2g0328971 67.073 246 74 3 300 539 158 402 2.79e-108 337
Msa1297230 MtrunA17_Chr7g0249621 77.723 202 42 1 298 496 248 449 2.96e-107 339
Msa1297230 MtrunA17_Chr5g0412151 80.108 186 34 1 300 482 215 400 4.70e-95 302
Msa1297230 MtrunA17_Chr4g0073521 71.084 166 48 0 308 473 46 211 3.05e-82 263
Msa1297230 MtrunA17_Chr7g0216601 62.366 186 66 2 291 473 48 232 3.68e-75 246
Msa1297230 MtrunA17_Chr8g0355191 63.636 176 63 1 308 483 54 228 7.58e-74 243
Msa1297230 MtrunA17_Chr6g0452911 61.340 194 65 1 290 473 42 235 1.50e-73 241
Msa1297230 MtrunA17_Chr4g0002011 68.639 169 50 1 308 473 35 203 4.43e-71 233
Msa1297230 MtrunA17_Chr8g0355181 50.204 245 115 3 308 552 55 292 1.32e-69 231
Msa1297230 MtrunA17_Chr8g0362541 49.612 258 114 3 262 505 23 278 1.76e-69 232
Msa1297230 MtrunA17_Chr8g0367311 70.548 146 43 0 346 491 22 167 4.62e-69 225
Msa1297230 MtrunA17_Chr2g0305821 58.333 144 48 2 278 419 24 157 2.32e-44 155
Msa1297230 MtrunA17_Chr4g0053181 53.333 165 69 3 312 476 176 332 4.04e-41 157
Msa1297230 MtrunA17_Chr7g0257361 63.077 130 40 2 307 433 55 179 6.40e-41 154
Msa1297230 MtrunA17_Chr5g0401891 53.333 165 69 3 312 476 152 308 1.70e-40 155
Msa1297230 MtrunA17_Chr4g0030191 49.419 172 79 3 312 483 131 294 9.91e-39 149
Msa1297230 MtrunA17_Chr7g0263581 50.303 165 74 3 312 476 139 295 1.22e-38 149
Msa1297230 MtrunA17_Chr2g0325911 46.667 165 65 4 312 476 134 275 3.86e-30 124
Msa1297230 MtrunA17_Chr2g0305831 73.684 57 15 0 417 473 3 59 2.39e-23 98.6
Msa1297230 MtrunA17_Chr3g0126151 47.368 95 49 1 357 451 47 140 4.84e-22 92.8
Msa1297230 MtrunA17_Chr8g0367321 48.214 112 43 4 240 347 93 193 2.70e-21 92.4
Msa1297230 MtrunA17_Chr1g0170761 39.007 141 78 3 288 428 113 245 7.52e-16 80.1
Msa1297230 MtrunA17_Chr1g0158971 49.315 73 36 1 396 468 99 170 1.56e-11 66.2
Msa1297230 MtrunA17_Chr4g0044901 35.514 107 64 3 412 515 96 200 2.55e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297230 AT4G37750.1 51.978 556 171 19 1 522 1 494 1.32e-156 463
Msa1297230 AT1G72570.1 71.721 244 63 3 252 491 164 405 3.87e-119 362
Msa1297230 AT5G57390.2 82.979 188 28 2 300 484 73 259 2.33e-110 340
Msa1297230 AT1G51190.1 66.535 254 77 4 248 496 128 378 3.39e-110 343
Msa1297230 AT3G20840.1 65.399 263 59 5 248 496 125 369 1.87e-109 342
Msa1297230 AT5G57390.1 82.979 188 28 2 300 484 191 377 2.39e-109 341
Msa1297230 AT5G17430.2 63.235 272 92 3 300 568 136 402 5.92e-107 334
Msa1297230 AT5G17430.1 63.235 272 92 3 300 568 198 464 1.50e-106 335
Msa1297230 AT5G10510.2 73.737 198 52 0 285 482 203 400 5.12e-92 295
Msa1297230 AT5G65510.4 69.014 213 63 1 285 494 146 358 6.45e-91 289
Msa1297230 AT5G65510.2 69.014 213 63 1 285 494 204 416 7.88e-91 291
Msa1297230 AT5G65510.1 69.014 213 63 1 285 494 204 416 7.88e-91 291
Msa1297230 AT5G10510.7 72.637 201 52 1 285 482 203 403 4.41e-90 290
Msa1297230 AT5G10510.5 72.637 201 52 1 285 482 203 403 4.41e-90 290
Msa1297230 AT5G10510.1 72.637 201 52 1 285 482 226 426 7.99e-90 290
Msa1297230 AT5G10510.6 72.637 201 52 1 285 482 241 441 9.13e-90 291
Msa1297230 AT5G10510.4 72.637 201 52 1 285 482 241 441 9.13e-90 291
Msa1297230 AT5G10510.3 65.611 221 53 1 285 482 241 461 1.47e-85 280
Msa1297230 AT5G65510.3 71.429 161 43 1 285 442 146 306 6.90e-79 253
Msa1297230 AT3G54320.3 68.047 169 51 1 308 473 61 229 1.60e-68 230
Msa1297230 AT3G54320.1 68.047 169 51 1 308 473 61 229 1.88e-68 230
Msa1297230 AT1G79700.2 67.456 169 52 1 308 473 48 216 1.43e-62 211
Msa1297230 AT1G16060.1 66.474 173 55 1 304 473 50 222 4.58e-61 207
Msa1297230 AT1G79700.1 63.314 169 49 2 308 473 48 206 8.36e-55 189
Msa1297230 AT3G54320.4 70.312 128 38 0 346 473 36 163 5.50e-51 181
Msa1297230 AT3G54320.5 70.312 128 38 0 346 473 36 163 5.50e-51 181
Msa1297230 AT1G79700.4 67.969 128 41 0 346 473 7 134 3.58e-43 155
Msa1297230 AT1G79700.3 67.969 128 41 0 346 473 13 140 3.69e-43 155
Msa1297230 AT1G16060.3 66.418 134 45 0 340 473 19 152 5.59e-42 154
Msa1297230 AT4G36920.1 42.449 245 112 5 236 476 68 287 2.50e-41 156
Msa1297230 AT4G36920.2 42.449 245 112 5 236 476 68 287 2.50e-41 156
Msa1297230 AT5G67180.3 48.851 174 81 3 312 485 96 261 1.04e-40 150
Msa1297230 AT2G41710.1 55.921 152 56 3 285 433 46 189 1.31e-40 154
Msa1297230 AT5G67180.2 49.143 175 81 3 312 486 96 262 1.75e-40 150
Msa1297230 AT2G41710.4 55.921 152 56 3 285 433 46 189 2.84e-40 154
Msa1297230 AT5G67180.1 49.143 175 81 3 312 486 96 262 4.14e-40 150
Msa1297230 AT2G41710.2 54.140 157 56 4 285 433 46 194 3.40e-38 147
Msa1297230 AT2G28550.3 43.111 225 97 8 312 526 153 356 1.61e-37 146
Msa1297230 AT2G41710.3 59.504 121 41 2 316 433 109 224 4.84e-34 135
Msa1297230 AT3G54320.2 72.727 88 24 0 386 473 1 88 7.36e-32 125
Msa1297230 AT2G28550.4 49.275 138 62 3 312 449 153 282 5.58e-31 124
Msa1297230 AT1G16060.2 70.455 88 26 0 386 473 1 88 5.72e-31 120
Msa1297230 AT1G16060.4 70.455 88 26 0 386 473 1 88 5.72e-31 120
Msa1297230 AT1G16060.6 70.455 88 26 0 386 473 1 88 5.72e-31 120
Msa1297230 AT1G16060.5 70.455 88 26 0 386 473 1 88 5.72e-31 120
Msa1297230 AT5G67180.4 48.252 143 66 3 312 454 96 230 3.46e-30 120
Msa1297230 AT2G28550.1 40.000 225 89 9 312 526 153 341 1.62e-29 122
Msa1297230 AT2G28550.2 49.194 124 55 3 312 435 153 268 2.37e-26 109
Msa1297230 AT2G28550.6 49.194 124 55 3 312 435 153 268 2.37e-26 109
Msa1297230 AT2G39250.2 42.202 109 56 2 313 421 109 210 4.65e-23 99.0
Msa1297230 AT2G39250.1 38.760 129 72 2 293 421 89 210 1.33e-22 99.8
Msa1297230 AT2G28550.5 38.743 191 78 7 346 526 14 175 2.24e-21 95.1
Msa1297230 AT5G60120.4 45.238 126 61 3 309 434 157 274 1.28e-19 92.0
Msa1297230 AT5G60120.3 45.238 126 61 3 309 434 157 274 1.28e-19 92.0
Msa1297230 AT5G60120.5 45.238 126 61 3 309 434 157 274 1.28e-19 92.0
Msa1297230 AT5G60120.6 39.873 158 72 5 309 466 157 291 2.03e-19 92.4
Msa1297230 AT5G60120.1 39.873 158 72 5 309 466 157 291 2.35e-19 92.0
Msa1297230 AT5G60120.2 39.873 158 72 5 309 466 157 291 3.01e-19 92.0
Msa1297230 AT3G54990.2 46.154 104 49 2 312 415 122 218 3.67e-18 85.1
Msa1297230 AT3G54990.1 46.154 104 49 2 312 415 122 218 1.91e-17 84.7
Msa1297230 AT1G16060.7 77.193 57 13 0 417 473 10 66 4.26e-16 77.4
Msa1297230 AT1G03800.1 53.226 62 28 1 412 473 53 113 7.64e-12 66.2
Msa1297230 AT5G65130.1 34.752 141 68 5 380 497 72 211 8.44e-11 63.9
Msa1297230 AT5G65130.2 34.752 141 68 5 380 497 74 213 9.11e-11 63.5

Find 129 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGCCTTAGTTAGTATATA+AGG 0.205288 8_3:-57223757 None:intergenic
TAAAGGATTGTAAAATGTTT+AGG 0.209265 8_3:-57227431 None:intergenic
CAAATAGTAATTTGAATTCT+TGG 0.209522 8_3:+57227061 Msa1297230:CDS
TCTACAATACCATGTCTTAA+AGG 0.217384 8_3:-57227448 None:intergenic
CAAGAATTCAAATTACTATT+TGG 0.219550 8_3:-57227060 None:intergenic
AGTAACTATTGTTACTCTTA+TGG 0.248617 8_3:+57224118 Msa1297230:CDS
AAAGGATTGTAAAATGTTTA+GGG 0.256300 8_3:-57227430 None:intergenic
AGGAAATCTATTGATACTTT+TGG 0.282332 8_3:+57224916 Msa1297230:CDS
TTCAACAGCTTCAAAACTAT+TGG 0.285703 8_3:+57227026 Msa1297230:CDS
ATATATCTATAAACTCTCTT+TGG 0.290703 8_3:-57223786 None:intergenic
AATATTGGTTCAATGGCTTA+TGG 0.295832 8_3:+57224736 Msa1297230:CDS
AATGCTAGGTCTTCATTCTA+TGG 0.296635 8_3:-57223664 None:intergenic
GGAGATATGAAGCTCATCTA+TGG 0.306792 8_3:+57225099 Msa1297230:CDS
CTAAAATGTAGTTACTAAAA+AGG 0.307240 8_3:-57227390 None:intergenic
GGGACCCTTCACAACATCTT+AGG 0.319809 8_3:+57227082 Msa1297230:CDS
ATAAGCCATTGAACCAATAT+TGG 0.320548 8_3:-57224734 None:intergenic
AATTTCAATGCAGAAGAATA+TGG 0.321865 8_3:+57226759 Msa1297230:CDS
AACCATGAGTTGATGTTAGA+AGG 0.326730 8_3:+57224538 Msa1297230:CDS
GTTCTTTCATTCTCAAATCT+TGG 0.331942 8_3:-57226826 None:intergenic
CTGTTGAAGTTGTTGATGTT+GGG 0.335268 8_3:-57226797 None:intergenic
GATATATTTCATTACATTGC+TGG 0.337570 8_3:+57223802 Msa1297230:five_prime_UTR
TCTCCTCAAATGAATAATAT+TGG 0.338994 8_3:+57223992 Msa1297230:CDS
ACATTAACAATGAAAATAAC+TGG 0.340271 8_3:+57223954 Msa1297230:CDS
AAATAGTAATTTGAATTCTT+GGG 0.341154 8_3:+57227062 Msa1297230:CDS
GACTTAGTGGTTTAAAGAAC+TGG 0.346259 8_3:+57224623 Msa1297230:CDS
ATCAGATTTGAGAGGCATAA+TGG 0.354338 8_3:-57224179 None:intergenic
TCAAGCAGCACTCAAGTATT+GGG 0.359648 8_3:+57225635 Msa1297230:CDS
CTTGCCATCTTCCATGTTGA+TGG 0.372286 8_3:-57226104 None:intergenic
GGTAATAAAGATCTATATCT+TGG 0.375013 8_3:+57226147 Msa1297230:CDS
AAAGAAGGCCAAAGCAGAAA+AGG 0.375414 8_3:+57225137 Msa1297230:CDS
TGTATCAAATGCTTCTTCAT+TGG 0.379502 8_3:+57226930 Msa1297230:CDS
TGATGGATCTCTTTATGAAA+TGG 0.383299 8_3:+57224198 Msa1297230:CDS
GAAGACCTAGCATTGCTTGC+TGG 0.391924 8_3:+57223673 Msa1297230:five_prime_UTR
TCTGTTGAAGTTGTTGATGT+TGG 0.394185 8_3:-57226798 None:intergenic
AGCAGAAGCAAATTGTTCAT+AGG 0.397213 8_3:+57224896 Msa1297230:CDS
GCTGGTTAGATACTTAGATA+TGG 0.409207 8_3:+57223820 Msa1297230:five_prime_UTR
AACAATGAAAATAACTGGTT+AGG 0.421756 8_3:+57223959 Msa1297230:CDS
TGGATCCAATATTGGTTCAA+TGG 0.423285 8_3:+57224729 Msa1297230:CDS
GGTGAAGATCATGGACTTAG+TGG 0.425302 8_3:+57224610 Msa1297230:CDS
AAGGGTATATGTATATCTAA+AGG 0.426431 8_3:-57223738 None:intergenic
GTGCCTTAGTTAGTATATAA+GGG 0.433582 8_3:-57223756 None:intergenic
AGGGTATATGTATATCTAAA+GGG 0.435527 8_3:-57223737 None:intergenic
TCAGATTTGAGAGGCATAAT+GGG 0.436788 8_3:-57224178 None:intergenic
TTTATTAGGCATAGGTGGAC+TGG 0.438736 8_3:+57225077 Msa1297230:intron
ATCAAGCAGCACTCAAGTAT+TGG 0.444500 8_3:+57225634 Msa1297230:CDS
ACACCAATATTATTCATTTG+AGG 0.447211 8_3:-57223995 None:intergenic
TATAATATGACAACTGTTGT+TGG 0.447627 8_3:-57224501 None:intergenic
TCAACATTGAACAAGCTAGA+AGG 0.451377 8_3:+57226647 Msa1297230:CDS
GGTGAATGTGGATCCAATAT+TGG 0.454709 8_3:+57224721 Msa1297230:CDS
GAGGGACAATATGTAAGAAA+TGG 0.454968 8_3:+57227236 Msa1297230:three_prime_UTR
GATGGGAGATAGTAAAATTG+AGG 0.459317 8_3:+57227260 Msa1297230:three_prime_UTR
GGAAGATGGCAAGCTCGAAT+TGG 0.460222 8_3:+57226114 Msa1297230:CDS
CCTTGTTACTCCTCTGTACA+TGG 0.462036 8_3:-57225964 None:intergenic
ACAAGCTTGCAAAATCTCTC+TGG 0.463000 8_3:+57226994 Msa1297230:CDS
TGTTGAAAATTCTGTTGCTA+TGG 0.468546 8_3:+57224843 Msa1297230:CDS
CACAAGATAACAGAATTTCA+AGG 0.474506 8_3:-57223888 None:intergenic
ATGCTAGGTCTTCATTCTAT+GGG 0.475763 8_3:-57223663 None:intergenic
TGTGATAGAGAACAAAAGAA+AGG 0.478820 8_3:-57227161 None:intergenic
TCAAACATGGGTGAAGATCA+TGG 0.480026 8_3:+57224601 Msa1297230:CDS
CAAAGAACATCTCAATATAG+AGG 0.486736 8_3:+57224940 Msa1297230:CDS
ATGAAATGGAAGCTCTTAGC+AGG 0.491080 8_3:+57224212 Msa1297230:CDS
AATATGTTGCTCATTTAAGA+AGG 0.492255 8_3:+57225839 Msa1297230:CDS
GAGAGAAAATATGAAGAGAA+TGG 0.493769 8_3:+57223910 Msa1297230:exon
CAAAGCAGAAAAGGAAGACA+AGG 0.499730 8_3:+57225146 Msa1297230:CDS
CTGGCATCTGAGGCAAAGAA+AGG 0.499839 8_3:-57227110 None:intergenic
GCTCGAATTGGTAGAGTAGC+TGG 0.499873 8_3:+57226126 Msa1297230:CDS
ATACCCTTATATACTAACTA+AGG 0.501254 8_3:+57223753 Msa1297230:five_prime_UTR
GAGTAACAATAGTTACTAAG+AGG 0.502157 8_3:-57224112 None:intergenic
TTGTGTCAAAGCATGTTTCG+AGG 0.505861 8_3:+57227349 Msa1297230:three_prime_UTR
TGATCATGTCCATGACTAGC+TGG 0.509563 8_3:-57224661 None:intergenic
ATGGGAGATAGTAAAATTGA+GGG 0.510372 8_3:+57227261 Msa1297230:three_prime_UTR
GGACTTGAACATGAAAATGC+TGG 0.514334 8_3:+57224139 Msa1297230:CDS
GTGATAGAGAACAAAAGAAA+GGG 0.521763 8_3:-57227160 None:intergenic
TTGTTGTCATATGCAGAAGA+AGG 0.522088 8_3:-57226721 None:intergenic
TAGCTTGTTCAATGTTGAGA+AGG 0.523772 8_3:-57226642 None:intergenic
AATGAAGAATATGACAAGAC+AGG 0.524291 8_3:+57225816 Msa1297230:CDS
TTATTAGGCATAGGTGGACT+GGG 0.524849 8_3:+57225078 Msa1297230:intron
TGGTAAAAGCTTGCTTGAAG+TGG 0.527376 8_3:-57224082 None:intergenic
GCAGAAGCAGAAGCAGAAGA+AGG 0.529348 8_3:-57224046 None:intergenic
GTACTACTACATGCATAATG+AGG 0.531601 8_3:-57224409 None:intergenic
ATTATGCCTCTCAAATCTGA+TGG 0.532902 8_3:+57224181 Msa1297230:CDS
AAGCAAAGTCAAACTTCAAG+TGG 0.535525 8_3:+57224565 Msa1297230:CDS
GACACCATCAACATGGAAGA+TGG 0.537820 8_3:+57226100 Msa1297230:intron
ACCCTTCTAACATCAACTCA+TGG 0.539230 8_3:-57224540 None:intergenic
ACCATGAGTTGATGTTAGAA+GGG 0.549490 8_3:+57224539 Msa1297230:CDS
TACTACTACATGCATAATGA+GGG 0.551148 8_3:-57224408 None:intergenic
GCTATGGATACAAAGAAAAG+AGG 0.553448 8_3:+57224859 Msa1297230:CDS
AATTGTTTATTAGGCATAGG+TGG 0.555057 8_3:+57225072 Msa1297230:intron
TGGCATCTGAGGCAAAGAAA+GGG 0.555089 8_3:-57227109 None:intergenic
GATGATTGTTCCTGTTGTAG+AGG 0.558499 8_3:+57224690 Msa1297230:CDS
TTGAAGTTGTTGATGTTGGG+AGG 0.565145 8_3:-57226794 None:intergenic
ACTTAGTGGTTTAAAGAACT+GGG 0.566240 8_3:+57224624 Msa1297230:CDS
GACAACAGTTGCAAGAAAGA+AGG 0.566962 8_3:+57225122 Msa1297230:CDS
CAATATGTAAGAAATGGTGA+TGG 0.568192 8_3:+57227242 Msa1297230:three_prime_UTR
GTATTCCAGCAAGCAATGCT+AGG 0.570286 8_3:-57223678 None:intergenic
GGGAAGTTTATGTGAGTGGA+TGG 0.574919 8_3:-57225660 None:intergenic
AACAGGGAAGTTTATGTGAG+TGG 0.577161 8_3:-57225664 None:intergenic
ACACAGCTAGACTGAGAGCT+AGG 0.578119 8_3:-57224790 None:intergenic
GAGATATGAAGCTCATCTAT+GGG 0.580404 8_3:+57225100 Msa1297230:CDS
AATATGTAAGAAATGGTGAT+GGG 0.581590 8_3:+57227243 Msa1297230:three_prime_UTR
AGTTTGAGCAGTTCAAGAGA+AGG 0.582795 8_3:+57226958 Msa1297230:CDS
GAAGGCCTAAGATGTTGTGA+AGG 0.585697 8_3:-57227087 None:intergenic
GAGGAAAATGAAGGTGAATG+TGG 0.588553 8_3:+57224709 Msa1297230:CDS
AAAAGCAGTGGATTCTCAAG+AGG 0.594604 8_3:+57225936 Msa1297230:CDS
AAAGCAGTGGATTCTCAAGA+GGG 0.594710 8_3:+57225937 Msa1297230:CDS
CTCAATATAGAGGTGTAACA+AGG 0.612520 8_3:+57224950 Msa1297230:CDS
GTTTCATCAAGAAGTAGTTG+AGG 0.620030 8_3:+57226885 Msa1297230:CDS
AACATGCTGTCTAAGCTCAA+AGG 0.623476 8_3:-57224448 None:intergenic
GTGAAGGTCTTTCTCATACA+CGG 0.626154 8_3:+57223637 Msa1297230:five_prime_UTR
AAATTATCAAAATCAACTTG+AGG 0.631469 8_3:+57225792 Msa1297230:CDS
GGTAGCAGACACCATCAACA+TGG 0.639807 8_3:+57226093 Msa1297230:intron
CCATGTACAGAGGAGTAACA+AGG 0.640756 8_3:+57225964 Msa1297230:CDS
GTAGAATAAAGCAAAAGTGA+AGG 0.641419 8_3:+57223621 None:intergenic
ATTGCAGCTATAAAATTCAG+AGG 0.642745 8_3:+57226553 Msa1297230:CDS
ATCTGAGGCAAAGAAAGGGA+AGG 0.647053 8_3:-57227105 None:intergenic
AGTTACTAAGAGGAGAAGAG+TGG 0.647916 8_3:-57224102 None:intergenic
AAGGCCTAAGATGTTGTGAA+GGG 0.651408 8_3:-57227086 None:intergenic
CCTGTTGTAGAGGAAAATGA+AGG 0.654487 8_3:+57224700 Msa1297230:CDS
CAAGCAGCACTCAAGTATTG+GGG 0.655625 8_3:+57225636 Msa1297230:CDS
AGAGATCCATCAGATTTGAG+AGG 0.660630 8_3:-57224187 None:intergenic
CTTAGCAGGTCACAAACACA+AGG 0.664909 8_3:+57224226 Msa1297230:CDS
TGCTAGGTCTTCATTCTATG+GGG 0.667132 8_3:-57223662 None:intergenic
AGCTAGAAGGAACAAAGAGG+TGG 0.670383 8_3:+57226660 Msa1297230:CDS
AAGCAGTGGATTCTCAAGAG+GGG 0.699895 8_3:+57225938 Msa1297230:CDS
AGAGGGGCTTCCATGTACAG+AGG 0.703240 8_3:+57225954 Msa1297230:CDS
AGCAGATGACATATTCTGTG+AGG 0.705618 8_3:-57224818 None:intergenic
ACTACTACATGCATAATGAG+GGG 0.710323 8_3:-57224407 None:intergenic
ACAAGCTAGAAGGAACAAAG+AGG 0.715606 8_3:+57226657 Msa1297230:CDS
CATGTTATCCAAAGACACTG+AGG 0.754091 8_3:-57226863 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTAATTATATTTTAA+TGG - chr8_3:57226022-57226041 None:intergenic 0.0%
!!! ATATTTTATTATATCAAAGT+TGG - chr8_3:57226593-57226612 None:intergenic 10.0%
!!! ATTCTTTTTGTTTAGTTTAA+AGG + chr8_3:57223701-57223720 Msa1297230:five_prime_UTR 15.0%
!!! TATGTTTTGAATTGTTTATT+AGG + chr8_3:57225063-57225082 Msa1297230:intron 15.0%
!!! TTTTGACAAAAACTATATTT+TGG - chr8_3:57225225-57225244 None:intergenic 15.0%
!! AACAAAATAAAAAATGAGAA+TGG - chr8_3:57225377-57225396 None:intergenic 15.0%
!!! ATTCCAAAATTTTGATTTTA+GGG + chr8_3:57225574-57225593 Msa1297230:intron 15.0%
!! CAATTAATAAACCTTATAAA+TGG - chr8_3:57225735-57225754 None:intergenic 15.0%
!! AGTACATTACAATATTAAAT+TGG - chr8_3:57226230-57226249 None:intergenic 15.0%
!! AAATAGTAATTTGAATTCTT+GGG + chr8_3:57227062-57227081 Msa1297230:CDS 15.0%
!! ATATATCTATAAACTCTCTT+TGG - chr8_3:57223789-57223808 None:intergenic 20.0%
!! ACATTAACAATGAAAATAAC+TGG + chr8_3:57223954-57223973 Msa1297230:CDS 20.0%
!! ATTATGTGTGTATTAATGTA+TGG + chr8_3:57224271-57224290 Msa1297230:intron 20.0%
!! CACAAAAATAGATAAAAAGT+GGG - chr8_3:57224324-57224343 None:intergenic 20.0%
!! TCACAAAAATAGATAAAAAG+TGG - chr8_3:57224325-57224344 None:intergenic 20.0%
!!! TTTTTTAGGTAATGAAACTA+TGG + chr8_3:57224381-57224400 Msa1297230:CDS 20.0%
!!! TTTTTAGGTAATGAAACTAT+GGG + chr8_3:57224382-57224401 Msa1297230:CDS 20.0%
!!! TGTTTTGTTGAATTTGATTT+TGG - chr8_3:57224474-57224493 None:intergenic 20.0%
!!! AAATTTTCATCAATCAAACA+TGG + chr8_3:57224588-57224607 Msa1297230:CDS 20.0%
!!! AATTTTCATCAATCAAACAT+GGG + chr8_3:57224589-57224608 Msa1297230:CDS 20.0%
!!! CATTCCAAAATTTTGATTTT+AGG + chr8_3:57225573-57225592 Msa1297230:intron 20.0%
!!! TTCCAAAATTTTGATTTTAG+GGG + chr8_3:57225575-57225594 Msa1297230:intron 20.0%
!!! AATGATTGTTATTACTAACA+GGG - chr8_3:57225683-57225702 None:intergenic 20.0%
!!! TAATGATTGTTATTACTAAC+AGG - chr8_3:57225684-57225703 None:intergenic 20.0%
!! TTGTTTCAATTCCATTTATA+AGG + chr8_3:57225721-57225740 Msa1297230:intron 20.0%
!! AAATTATCAAAATCAACTTG+AGG + chr8_3:57225792-57225811 Msa1297230:CDS 20.0%
!!! CTTGGATTATATTTTTACTT+GGG - chr8_3:57226448-57226467 None:intergenic 20.0%
!! ATATGATGTTGAAAAAATCA+TGG + chr8_3:57226609-57226628 Msa1297230:CDS 20.0%
!! CAAGAATTCAAATTACTATT+TGG - chr8_3:57227063-57227082 None:intergenic 20.0%
!! CAAATAGTAATTTGAATTCT+TGG + chr8_3:57227061-57227080 Msa1297230:CDS 20.0%
!! CTAAAATGTAGTTACTAAAA+AGG - chr8_3:57227393-57227412 None:intergenic 20.0%
!! AAAGGATTGTAAAATGTTTA+GGG - chr8_3:57227433-57227452 None:intergenic 20.0%
!! TAAAGGATTGTAAAATGTTT+AGG - chr8_3:57227434-57227453 None:intergenic 20.0%
! AGGGTATATGTATATCTAAA+GGG - chr8_3:57223740-57223759 None:intergenic 25.0%
!! AAGGGTATATGTATATCTAA+AGG - chr8_3:57223741-57223760 None:intergenic 25.0%
! ATACCCTTATATACTAACTA+AGG + chr8_3:57223753-57223772 Msa1297230:five_prime_UTR 25.0%
! GATATATTTCATTACATTGC+TGG + chr8_3:57223802-57223821 Msa1297230:five_prime_UTR 25.0%
! AACAATGAAAATAACTGGTT+AGG + chr8_3:57223959-57223978 Msa1297230:CDS 25.0%
! TCTCCTCAAATGAATAATAT+TGG + chr8_3:57223992-57224011 Msa1297230:CDS 25.0%
! ACACCAATATTATTCATTTG+AGG - chr8_3:57223998-57224017 None:intergenic 25.0%
! AGTAACTATTGTTACTCTTA+TGG + chr8_3:57224118-57224137 Msa1297230:CDS 25.0%
!!! CCTAAAAAATTCTCTAGTTT+TGG - chr8_3:57224370-57224389 None:intergenic 25.0%
!!! CCAAAACTAGAGAATTTTTT+AGG + chr8_3:57224367-57224386 Msa1297230:CDS 25.0%
!!! TTTTAGGTAATGAAACTATG+GGG + chr8_3:57224383-57224402 Msa1297230:CDS 25.0%
! TATAATATGACAACTGTTGT+TGG - chr8_3:57224504-57224523 None:intergenic 25.0%
!! AGGAAATCTATTGATACTTT+TGG + chr8_3:57224916-57224935 Msa1297230:CDS 25.0%
! TTGAATTGTTTATTAGGCAT+AGG + chr8_3:57225069-57225088 Msa1297230:intron 25.0%
! AATGATACTATGTGAACATT+TGG + chr8_3:57225173-57225192 Msa1297230:intron 25.0%
!!! TTTGTTATTAGATTCTGTCT+TGG + chr8_3:57225499-57225518 Msa1297230:intron 25.0%
!!! TTGCTTATGTTTTTAACATG+AGG + chr8_3:57225529-57225548 Msa1297230:intron 25.0%
!!! TCCAAAATTTTGATTTTAGG+GGG + chr8_3:57225576-57225595 Msa1297230:intron 25.0%
! TTTCATTCTCTTATAACAGT+TGG + chr8_3:57225768-57225787 Msa1297230:intron 25.0%
! AATATGTTGCTCATTTAAGA+AGG + chr8_3:57225839-57225858 Msa1297230:CDS 25.0%
! GGTAATAAAGATCTATATCT+TGG + chr8_3:57226147-57226166 Msa1297230:CDS 25.0%
! TTAAATTGGTACATGATCAA+TGG - chr8_3:57226216-57226235 None:intergenic 25.0%
!!! CCTTGGATTATATTTTTACT+TGG - chr8_3:57226449-57226468 None:intergenic 25.0%
! CCAAGTAAAAATATAATCCA+AGG + chr8_3:57226446-57226465 Msa1297230:intron 25.0%
!! CTCATTGAAATGAAACTTTT+AGG + chr8_3:57226499-57226518 Msa1297230:intron 25.0%
! AATTTCAATGCAGAAGAATA+TGG + chr8_3:57226759-57226778 Msa1297230:CDS 25.0%
!!! TCTTTCTTTTACTTTTTGCA+TGG + chr8_3:57227214-57227233 Msa1297230:three_prime_UTR 25.0%
! AATATGTAAGAAATGGTGAT+GGG + chr8_3:57227243-57227262 Msa1297230:three_prime_UTR 25.0%
!!! TTTTACAATCCTTTAAGACA+TGG + chr8_3:57227439-57227458 Msa1297230:three_prime_UTR 25.0%
GTGCCTTAGTTAGTATATAA+GGG - chr8_3:57223759-57223778 None:intergenic 30.0%
TGTGCCTTAGTTAGTATATA+AGG - chr8_3:57223760-57223779 None:intergenic 30.0%
CACAAGATAACAGAATTTCA+AGG - chr8_3:57223891-57223910 None:intergenic 30.0%
GAGAGAAAATATGAAGAGAA+TGG + chr8_3:57223910-57223929 Msa1297230:exon 30.0%
GAGTAACAATAGTTACTAAG+AGG - chr8_3:57224115-57224134 None:intergenic 30.0%
! TGATGGATCTCTTTATGAAA+TGG + chr8_3:57224198-57224217 Msa1297230:CDS 30.0%
TACTACTACATGCATAATGA+GGG - chr8_3:57224411-57224430 None:intergenic 30.0%
ACTTAGTGGTTTAAAGAACT+GGG + chr8_3:57224624-57224643 Msa1297230:CDS 30.0%
! GTTTAAAGAACTGGGTTTTA+AGG + chr8_3:57224632-57224651 Msa1297230:CDS 30.0%
ATAAGCCATTGAACCAATAT+TGG - chr8_3:57224737-57224756 None:intergenic 30.0%
!! AATATTGGTTCAATGGCTTA+TGG + chr8_3:57224736-57224755 Msa1297230:CDS 30.0%
TGTTGAAAATTCTGTTGCTA+TGG + chr8_3:57224843-57224862 Msa1297230:CDS 30.0%
CAAAGAACATCTCAATATAG+AGG + chr8_3:57224940-57224959 Msa1297230:CDS 30.0%
AATTGTTTATTAGGCATAGG+TGG + chr8_3:57225072-57225091 Msa1297230:intron 30.0%
!!! TGTTTTTTGCTTTCTTTGCT+TGG + chr8_3:57225393-57225412 Msa1297230:intron 30.0%
! ACCCCCTAAAATCAAAATTT+TGG - chr8_3:57225580-57225599 None:intergenic 30.0%
TGGAATTGAAACAAAAGACA+TGG - chr8_3:57225715-57225734 None:intergenic 30.0%
AATGAAGAATATGACAAGAC+AGG + chr8_3:57225816-57225835 Msa1297230:CDS 30.0%
CTTTCAAAATCTTGTTCACT+TGG + chr8_3:57226072-57226091 Msa1297230:intron 30.0%
!! AATTTTGATCCCACAAACAT+TGG - chr8_3:57226424-57226443 None:intergenic 30.0%
! TTTAACTTTCATGCTAACCT+TGG - chr8_3:57226466-57226485 None:intergenic 30.0%
! TTTTATAGCTGCAATGTCAT+AGG - chr8_3:57226548-57226567 None:intergenic 30.0%
ATTGCAGCTATAAAATTCAG+AGG + chr8_3:57226553-57226572 Msa1297230:CDS 30.0%
GTTCTTTCATTCTCAAATCT+TGG - chr8_3:57226829-57226848 None:intergenic 30.0%
TGTATCAAATGCTTCTTCAT+TGG + chr8_3:57226930-57226949 Msa1297230:CDS 30.0%
!!! AATAGTTTTGAAGCTGTTGA+AGG - chr8_3:57227027-57227046 None:intergenic 30.0%
TTCAACAGCTTCAAAACTAT+TGG + chr8_3:57227026-57227045 Msa1297230:CDS 30.0%
GTGATAGAGAACAAAAGAAA+GGG - chr8_3:57227163-57227182 None:intergenic 30.0%
TGTGATAGAGAACAAAAGAA+AGG - chr8_3:57227164-57227183 None:intergenic 30.0%
!!! TTCTTTTACTTTTTGCATGG+AGG + chr8_3:57227217-57227236 Msa1297230:three_prime_UTR 30.0%
!!! TCTTTTACTTTTTGCATGGA+GGG + chr8_3:57227218-57227237 Msa1297230:three_prime_UTR 30.0%
CAATATGTAAGAAATGGTGA+TGG + chr8_3:57227242-57227261 Msa1297230:three_prime_UTR 30.0%
ATGGGAGATAGTAAAATTGA+GGG + chr8_3:57227261-57227280 Msa1297230:three_prime_UTR 30.0%
TCTACAATACCATGTCTTAA+AGG - chr8_3:57227451-57227470 None:intergenic 30.0%
ATGCTAGGTCTTCATTCTAT+GGG - chr8_3:57223666-57223685 None:intergenic 35.0%
AATGCTAGGTCTTCATTCTA+TGG - chr8_3:57223667-57223686 None:intergenic 35.0%
GCTGGTTAGATACTTAGATA+TGG + chr8_3:57223820-57223839 Msa1297230:five_prime_UTR 35.0%
! TCAGATTTGAGAGGCATAAT+GGG - chr8_3:57224181-57224200 None:intergenic 35.0%
! ATCAGATTTGAGAGGCATAA+TGG - chr8_3:57224182-57224201 None:intergenic 35.0%
ATTATGCCTCTCAAATCTGA+TGG + chr8_3:57224181-57224200 Msa1297230:CDS 35.0%
ACTACTACATGCATAATGAG+GGG - chr8_3:57224410-57224429 None:intergenic 35.0%
GTACTACTACATGCATAATG+AGG - chr8_3:57224412-57224431 None:intergenic 35.0%
!! AACCATGAGTTGATGTTAGA+AGG + chr8_3:57224538-57224557 Msa1297230:CDS 35.0%
!! ACCATGAGTTGATGTTAGAA+GGG + chr8_3:57224539-57224558 Msa1297230:CDS 35.0%
AAGCAAAGTCAAACTTCAAG+TGG + chr8_3:57224565-57224584 Msa1297230:CDS 35.0%
GACTTAGTGGTTTAAAGAAC+TGG + chr8_3:57224623-57224642 Msa1297230:CDS 35.0%
!! TGGATCCAATATTGGTTCAA+TGG + chr8_3:57224729-57224748 Msa1297230:CDS 35.0%
GCTATGGATACAAAGAAAAG+AGG + chr8_3:57224859-57224878 Msa1297230:CDS 35.0%
AGCAGAAGCAAATTGTTCAT+AGG + chr8_3:57224896-57224915 Msa1297230:CDS 35.0%
! CTCAATATAGAGGTGTAACA+AGG + chr8_3:57224950-57224969 Msa1297230:CDS 35.0%
GAGATATGAAGCTCATCTAT+GGG + chr8_3:57225100-57225119 Msa1297230:CDS 35.0%
! TTTTTGCTTTCTTTGCTTGG+CGG + chr8_3:57225396-57225415 Msa1297230:intron 35.0%
TGATGAGACACAGTTTATCT+TGG + chr8_3:57225444-57225463 Msa1297230:intron 35.0%
!! GATTTTAGGGGGTTATGATA+TGG + chr8_3:57225587-57225606 Msa1297230:intron 35.0%
GATCTTATCAGAAAAAGCAG+TGG + chr8_3:57225924-57225943 Msa1297230:intron 35.0%
GTGAGTTCTTTCATACTCTT+TGG + chr8_3:57226178-57226197 Msa1297230:intron 35.0%
TGTAATGTACTAGACACATG+TGG + chr8_3:57226239-57226258 Msa1297230:intron 35.0%
ATGTTCTGTTCCAATGTTTG+TGG + chr8_3:57226411-57226430 Msa1297230:intron 35.0%
TGTTCTGTTCCAATGTTTGT+GGG + chr8_3:57226412-57226431 Msa1297230:intron 35.0%
!! GAAACTTTTAGGTACTCAAG+AGG + chr8_3:57226510-57226529 Msa1297230:intron 35.0%
!! TAGCTTGTTCAATGTTGAGA+AGG - chr8_3:57226645-57226664 None:intergenic 35.0%
TCAACATTGAACAAGCTAGA+AGG + chr8_3:57226647-57226666 Msa1297230:CDS 35.0%
TTGTTGTCATATGCAGAAGA+AGG - chr8_3:57226724-57226743 None:intergenic 35.0%
CTGTTGAAGTTGTTGATGTT+GGG - chr8_3:57226800-57226819 None:intergenic 35.0%
TCTGTTGAAGTTGTTGATGT+TGG - chr8_3:57226801-57226820 None:intergenic 35.0%
!! AACTTTTTCCTCAGTGTCTT+TGG + chr8_3:57226855-57226874 Msa1297230:CDS 35.0%
GTTTCATCAAGAAGTAGTTG+AGG + chr8_3:57226885-57226904 Msa1297230:CDS 35.0%
!! TTTTGCAAGCTTGTTCTATC+TGG - chr8_3:57226988-57227007 None:intergenic 35.0%
GAGGGACAATATGTAAGAAA+TGG + chr8_3:57227236-57227255 Msa1297230:three_prime_UTR 35.0%
GATGGGAGATAGTAAAATTG+AGG + chr8_3:57227260-57227279 Msa1297230:three_prime_UTR 35.0%
GTGAAGGTCTTTCTCATACA+CGG + chr8_3:57223637-57223656 Msa1297230:five_prime_UTR 40.0%
TGCTAGGTCTTCATTCTATG+GGG - chr8_3:57223665-57223684 None:intergenic 40.0%
! TGGTAAAAGCTTGCTTGAAG+TGG - chr8_3:57224085-57224104 None:intergenic 40.0%
AGTTACTAAGAGGAGAAGAG+TGG - chr8_3:57224105-57224124 None:intergenic 40.0%
GGACTTGAACATGAAAATGC+TGG + chr8_3:57224139-57224158 Msa1297230:CDS 40.0%
AGAGATCCATCAGATTTGAG+AGG - chr8_3:57224190-57224209 None:intergenic 40.0%
ATGAAATGGAAGCTCTTAGC+AGG + chr8_3:57224212-57224231 Msa1297230:CDS 40.0%
AACATGCTGTCTAAGCTCAA+AGG - chr8_3:57224451-57224470 None:intergenic 40.0%
ACCCTTCTAACATCAACTCA+TGG - chr8_3:57224543-57224562 None:intergenic 40.0%
TCAAACATGGGTGAAGATCA+TGG + chr8_3:57224601-57224620 Msa1297230:CDS 40.0%
! AGGAATTTTCCAGCTAGTCA+TGG + chr8_3:57224652-57224671 Msa1297230:CDS 40.0%
GATGATTGTTCCTGTTGTAG+AGG + chr8_3:57224690-57224709 Msa1297230:CDS 40.0%
! CCTTCATTTTCCTCTACAAC+AGG - chr8_3:57224703-57224722 None:intergenic 40.0%
CCTGTTGTAGAGGAAAATGA+AGG + chr8_3:57224700-57224719 Msa1297230:CDS 40.0%
GAGGAAAATGAAGGTGAATG+TGG + chr8_3:57224709-57224728 Msa1297230:CDS 40.0%
GGTGAATGTGGATCCAATAT+TGG + chr8_3:57224721-57224740 Msa1297230:CDS 40.0%
AGCAGATGACATATTCTGTG+AGG - chr8_3:57224821-57224840 None:intergenic 40.0%
TTTATTAGGCATAGGTGGAC+TGG + chr8_3:57225077-57225096 Msa1297230:intron 40.0%
! TTATTAGGCATAGGTGGACT+GGG + chr8_3:57225078-57225097 Msa1297230:intron 40.0%
GGAGATATGAAGCTCATCTA+TGG + chr8_3:57225099-57225118 Msa1297230:CDS 40.0%
GACAACAGTTGCAAGAAAGA+AGG + chr8_3:57225122-57225141 Msa1297230:CDS 40.0%
AAAGAAGGCCAAAGCAGAAA+AGG + chr8_3:57225137-57225156 Msa1297230:CDS 40.0%
! CTTGTCTTCCTTTTCTGCTT+TGG - chr8_3:57225148-57225167 None:intergenic 40.0%
CAAAGCAGAAAAGGAAGACA+AGG + chr8_3:57225146-57225165 Msa1297230:CDS 40.0%
! GGATTCACACGATGATTTTG+CGG + chr8_3:57225194-57225213 Msa1297230:intron 40.0%
! TTTTGCTTTCTTTGCTTGGC+GGG + chr8_3:57225397-57225416 Msa1297230:intron 40.0%
ATCAAGCAGCACTCAAGTAT+TGG + chr8_3:57225634-57225653 Msa1297230:CDS 40.0%
TCAAGCAGCACTCAAGTATT+GGG + chr8_3:57225635-57225654 Msa1297230:CDS 40.0%
AACAGGGAAGTTTATGTGAG+TGG - chr8_3:57225667-57225686 None:intergenic 40.0%
! AAAAGCAGTGGATTCTCAAG+AGG + chr8_3:57225936-57225955 Msa1297230:CDS 40.0%
! AAAGCAGTGGATTCTCAAGA+GGG + chr8_3:57225937-57225956 Msa1297230:CDS 40.0%
! GAGGAGTAACAAGGTCTTAT+AGG + chr8_3:57225973-57225992 Msa1297230:intron 40.0%
CATTAGACATGACACAGACA+CGG - chr8_3:57226302-57226321 None:intergenic 40.0%
ACAAGCTAGAAGGAACAAAG+AGG + chr8_3:57226657-57226676 Msa1297230:CDS 40.0%
TTGAAGTTGTTGATGTTGGG+AGG - chr8_3:57226797-57226816 None:intergenic 40.0%
CATGTTATCCAAAGACACTG+AGG - chr8_3:57226866-57226885 None:intergenic 40.0%
AGTTTGAGCAGTTCAAGAGA+AGG + chr8_3:57226958-57226977 Msa1297230:CDS 40.0%
ACAAGCTTGCAAAATCTCTC+TGG + chr8_3:57226994-57227013 Msa1297230:CDS 40.0%
AAGGCCTAAGATGTTGTGAA+GGG - chr8_3:57227089-57227108 None:intergenic 40.0%
TTGTGTCAAAGCATGTTTCG+AGG + chr8_3:57227349-57227368 Msa1297230:three_prime_UTR 40.0%
GTATTCCAGCAAGCAATGCT+AGG - chr8_3:57223681-57223700 None:intergenic 45.0%
CTTAGCAGGTCACAAACACA+AGG + chr8_3:57224226-57224245 Msa1297230:CDS 45.0%
GGTGAAGATCATGGACTTAG+TGG + chr8_3:57224610-57224629 Msa1297230:CDS 45.0%
TGATCATGTCCATGACTAGC+TGG - chr8_3:57224664-57224683 None:intergenic 45.0%
! TCACACGATGATTTTGCGGA+CGG + chr8_3:57225198-57225217 Msa1297230:intron 45.0%
TTTGCTTTCTTTGCTTGGCG+GGG + chr8_3:57225398-57225417 Msa1297230:intron 45.0%
! CAAGCAGCACTCAAGTATTG+GGG + chr8_3:57225636-57225655 Msa1297230:CDS 45.0%
GGGAAGTTTATGTGAGTGGA+TGG - chr8_3:57225663-57225682 None:intergenic 45.0%
! AAGCAGTGGATTCTCAAGAG+GGG + chr8_3:57225938-57225957 Msa1297230:CDS 45.0%
CCTTGTTACTCCTCTGTACA+TGG - chr8_3:57225967-57225986 None:intergenic 45.0%
CCATGTACAGAGGAGTAACA+AGG + chr8_3:57225964-57225983 Msa1297230:CDS 45.0%
GACACCATCAACATGGAAGA+TGG + chr8_3:57226100-57226119 Msa1297230:intron 45.0%
CTTGCCATCTTCCATGTTGA+TGG - chr8_3:57226107-57226126 None:intergenic 45.0%
TATACGCGCACAACTACTAG+CGG - chr8_3:57226367-57226386 None:intergenic 45.0%
AGCTAGAAGGAACAAAGAGG+TGG + chr8_3:57226660-57226679 Msa1297230:CDS 45.0%
GAAGGCCTAAGATGTTGTGA+AGG - chr8_3:57227090-57227109 None:intergenic 45.0%
! ATCTGAGGCAAAGAAAGGGA+AGG - chr8_3:57227108-57227127 None:intergenic 45.0%
! TGGCATCTGAGGCAAAGAAA+GGG - chr8_3:57227112-57227131 None:intergenic 45.0%
! AGATGCCAGTTTTTGCAGCA+TGG + chr8_3:57227124-57227143 Msa1297230:CDS 45.0%
TCTGTCCATGCTGCAAAAAC+TGG - chr8_3:57227132-57227151 None:intergenic 45.0%
GAAGACCTAGCATTGCTTGC+TGG + chr8_3:57223673-57223692 Msa1297230:five_prime_UTR 50.0%
GCAGAAGCAGAAGCAGAAGA+AGG - chr8_3:57224049-57224068 None:intergenic 50.0%
ACACAGCTAGACTGAGAGCT+AGG - chr8_3:57224793-57224812 None:intergenic 50.0%
GGTAGCAGACACCATCAACA+TGG + chr8_3:57226093-57226112 Msa1297230:intron 50.0%
GGAAGATGGCAAGCTCGAAT+TGG + chr8_3:57226114-57226133 Msa1297230:CDS 50.0%
GCTCGAATTGGTAGAGTAGC+TGG + chr8_3:57226126-57226145 Msa1297230:CDS 50.0%
GGGACCCTTCACAACATCTT+AGG + chr8_3:57227082-57227101 Msa1297230:CDS 50.0%
! CTGGCATCTGAGGCAAAGAA+AGG - chr8_3:57227113-57227132 None:intergenic 50.0%
GCTGCAAAAACTGGCATCTG+AGG - chr8_3:57227123-57227142 None:intergenic 50.0%
AGAGGGGCTTCCATGTACAG+AGG + chr8_3:57225954-57225973 Msa1297230:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 57223630 57227481 57223630 ID=Msa1297230;Name=Msa1297230
chr8_3 mRNA 57223630 57227481 57223630 ID=Msa1297230-mRNA-1;Parent=Msa1297230;Name=Msa1297230-mRNA-1;_AED=0.12;_eAED=0.12;_QI=290|0.85|1|1|1|1|8|323|654
chr8_3 exon 57223630 57224247 57223630 ID=Msa1297230-mRNA-1:exon:16416;Parent=Msa1297230-mRNA-1
chr8_3 exon 57224347 57224971 57224347 ID=Msa1297230-mRNA-1:exon:16417;Parent=Msa1297230-mRNA-1
chr8_3 exon 57225085 57225167 57225085 ID=Msa1297230-mRNA-1:exon:16418;Parent=Msa1297230-mRNA-1
chr8_3 exon 57225595 57225683 57225595 ID=Msa1297230-mRNA-1:exon:16419;Parent=Msa1297230-mRNA-1
chr8_3 exon 57225787 57225860 57225787 ID=Msa1297230-mRNA-1:exon:16420;Parent=Msa1297230-mRNA-1
chr8_3 exon 57225935 57225985 57225935 ID=Msa1297230-mRNA-1:exon:16421;Parent=Msa1297230-mRNA-1
chr8_3 exon 57226101 57226177 57226101 ID=Msa1297230-mRNA-1:exon:16422;Parent=Msa1297230-mRNA-1
chr8_3 exon 57226521 57227481 57226521 ID=Msa1297230-mRNA-1:exon:16423;Parent=Msa1297230-mRNA-1
chr8_3 five_prime_UTR 57223630 57223919 57223630 ID=Msa1297230-mRNA-1:five_prime_utr;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57223920 57224247 57223920 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57224347 57224971 57224347 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57225085 57225167 57225085 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57225595 57225683 57225595 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57225787 57225860 57225787 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57225935 57225985 57225935 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57226101 57226177 57226101 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 CDS 57226521 57227158 57226521 ID=Msa1297230-mRNA-1:cds;Parent=Msa1297230-mRNA-1
chr8_3 three_prime_UTR 57227159 57227481 57227159 ID=Msa1297230-mRNA-1:three_prime_utr;Parent=Msa1297230-mRNA-1
Gene Sequence

>Msa1297230

ATGAAGAGAATGGAAAACAATGATGATAGTGTTGACATTAACAATGAAAATAACTGGTTAGGTTTTTCACTCTCTCCTCAAATGAATAATATTGGTGTTACTTCACACACTCATCATCATTCACTGCCTTCTTCTGCTTCTGCTTCTGCTTCTGAAGTAGTTCCACTTCAAGCAAGCTTTTACCACTCTTCTCCTCTTAGTAACTATTGTTACTCTTATGGACTTGAACATGAAAATGCTGGCTTGTATTCATTACTTCCCATTATGCCTCTCAAATCTGATGGATCTCTTTATGAAATGGAAGCTCTTAGCAGGTCACAAACACAAGCAATGAGTACTACTTCAGCACCAAAACTAGAGAATTTTTTAGGTAATGAAACTATGGGGACCCCTCATTATGCATGTAGTAGTACTGTCACAGAAACAATGCCTTTGAGCTTAGACAGCATGTTCCAAAATCAAATTCAACAAAACATGAACAACCAACAACAGTTGTCATATTATAACTCAACTATGAGAAACCATGAGTTGATGTTAGAAGGGTCAAAGCAAAGTCAAACTTCAAGTGGAAATTTTCATCAATCAAACATGGGTGAAGATCATGGACTTAGTGGTTTAAAGAACTGGGTTTTAAGGAATTTTCCAGCTAGTCATGGACATGATCAGTCAAAGATGATTGTTCCTGTTGTAGAGGAAAATGAAGGTGAATGTGGATCCAATATTGGTTCAATGGCTTATGGAGATTTGCATTCTTTGAGTTTGTCAATGAGTCCTAGCTCTCAGTCTAGCTGTGTTACAACCTCACAGAATATGTCATCTGCTGTTGTTGAAAATTCTGTTGCTATGGATACAAAGAAAAGAGGAAGTGAAAAATTTGAGCAGAAGCAAATTGTTCATAGGAAATCTATTGATACTTTTGGTCAAAGAACATCTCAATATAGAGGTGTAACAAGGCATAGGTGGACTGGGAGATATGAAGCTCATCTATGGGACAACAGTTGCAAGAAAGAAGGCCAAAGCAGAAAAGGAAGACAAGGGGGTTATGATATGGAAGAAAAAGCTGCTAGAGCTTATGATCAAGCAGCACTCAAGTATTGGGGACCATCCACTCACATAAACTTCCCTTTGGAAAATTATCAAAATCAACTTGAGGAAATGAAGAATATGACAAGACAGGAATATGTTGCTCATTTAAGAAGAAAAAGCAGTGGATTCTCAAGAGGGGCTTCCATGTACAGAGGAGTAACAAGACACCATCAACATGGAAGATGGCAAGCTCGAATTGGTAGAGTAGCTGGTAATAAAGATCTATATCTTGGAACTTTCAGTACTCAAGAGGAAGCAGCTGAAGCCTATGACATTGCAGCTATAAAATTCAGAGGAGCAAATGCAGTGACCAACTTTGATATAATAAAATATGATGTTGAAAAAATCATGGCTAGTAGTAACCTTCTCAACATTGAACAAGCTAGAAGGAACAAAGAGGTGGTAGATATGAGTACAACTCAATACATTGATCAAAACAAGCCTTCTTCTGCATATGACAACAATAGCACTCAAGAAGCAATTTCAATGCAGAAGAATATGGTTCTGTATCAATCCTCCCAACATCAACAACTTCAACAGAATCAACCAAGATTTGAGAATGAAAGAACTCATCAAACTTTTTCCTCAGTGTCTTTGGATAACATGTTTCATCAAGAAGTAGTTGAGGAAGCAAGTAAGATGAGAACACATGTATCAAATGCTTCTTCATTGGCTACAAGTTTGAGCAGTTCAAGAGAAGGTAGTCCAGATAGAACAAGCTTGCAAAATCTCTCTGGAATAATGCCTTCAACAGCTTCAAAACTATTGGTTACTTCTGCTCCAAATAGTAATTTGAATTCTTGGGACCCTTCACAACATCTTAGGCCTTCCCTTTCTTTGCCTCAGATGCCAGTTTTTGCAGCATGGACAGATGCTTAG
Protein sequence

>Msa1297230

MKRMENNDDSVDINNENNWLGFSLSPQMNNIGVTSHTHHHSLPSSASASASEVVPLQASFYHSSPLSNYCYSYGLEHENAGLYSLLPIMPLKSDGSLYEMEALSRSQTQAMSTTSAPKLENFLGNETMGTPHYACSSTVTETMPLSLDSMFQNQIQQNMNNQQQLSYYNSTMRNHELMLEGSKQSQTSSGNFHQSNMGEDHGLSGLKNWVLRNFPASHGHDQSKMIVPVVEENEGECGSNIGSMAYGDLHSLSLSMSPSSQSSCVTTSQNMSSAVVENSVAMDTKKRGSEKFEQKQIVHRKSIDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKEGQSRKGRQGGYDMEEKAARAYDQAALKYWGPSTHINFPLENYQNQLEEMKNMTRQEYVAHLRRKSSGFSRGASMYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDIAAIKFRGANAVTNFDIIKYDVEKIMASSNLLNIEQARRNKEVVDMSTTQYIDQNKPSSAYDNNSTQEAISMQKNMVLYQSSQHQQLQQNQPRFENERTHQTFSSVSLDNMFHQEVVEEASKMRTHVSNASSLATSLSSSREGSPDRTSLQNLSGIMPSTASKLLVTSAPNSNLNSWDPSQHLRPSLSLPQMPVFAAWTDA*