AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0748460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748460 MtrunA17_Chr1g0188131 95.413 109 2 1 1 106 1 109 6.63e-74 214
Msa0748460 MtrunA17_Chr1g0188161 79.817 109 18 3 1 106 1 108 1.63e-48 150
Msa0748460 MtrunA17_Chr1g0184941 53.846 104 47 1 3 106 7 109 7.01e-37 120
Msa0748460 MtrunA17_Chr1g0148341 40.217 92 52 2 16 105 35 125 4.77e-18 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0748460 AT3G63420.1 56.842 95 40 1 12 106 5 98 2.46e-32 109
Msa0748460 AT3G63420.2 56.842 95 40 1 12 106 5 98 2.46e-32 109
Msa0748460 AT3G22942.1 70.238 84 24 1 23 106 18 100 7.20e-32 108
Msa0748460 AT3G63420.3 56.842 95 40 1 12 106 45 138 1.23e-31 108

Find 71 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATCCCATATAGGATTTA+AGG 0.165452 5_2:+1702823 None:intergenic
ATTACATGGTCAACACTTAA+TGG 0.242126 5_2:-1705421 Msa0748460:five_prime_UTR
CAGGACCAATCTCGAATTAA+AGG 0.249997 5_2:+1702717 None:intergenic
TGCAGCAGCCTTTGGAATCT+TGG 0.250553 5_2:+1702786 None:intergenic
AAATAAACTCACAAAATTTC+AGG 0.285103 5_2:+1702698 None:intergenic
GCAGCAGCCTTTGGAATCTT+GGG 0.298135 5_2:+1702787 None:intergenic
TTGGTCCCTTAAATCCTATA+TGG 0.329222 5_2:-1702829 Msa0748460:CDS
CAACTGGTTTGAAATAGTTA+AGG 0.332083 5_2:-1702538 Msa0748460:three_prime_UTR
CTGTTGTTTCTTTCATTACA+TGG 0.337952 5_2:-1705435 Msa0748460:five_prime_UTR
TAAATCCTATATGGGATAGA+TGG 0.350762 5_2:-1702820 Msa0748460:CDS
GATCATTTAAGAAATGTAAA+TGG 0.351180 5_2:-1702575 Msa0748460:three_prime_UTR
AAAGTGGGTCGTCGGGTTAT+CGG 0.364117 5_2:+1705236 None:intergenic
TTACATGGTCAACACTTAAT+GGG 0.374330 5_2:-1705420 Msa0748460:five_prime_UTR
GACAGAGAAAGAGACAGTTT+TGG 0.379861 5_2:+1705484 None:intergenic
AAACAACAGAGCGGGATCTA+TGG 0.380122 5_2:+1705449 None:intergenic
AACTGGTTTGAAATAGTTAA+GGG 0.382241 5_2:-1702537 Msa0748460:three_prime_UTR
CCAACATCTGCAGCAGCCTT+TGG 0.388678 5_2:+1702778 None:intergenic
TTGAAATAGTTAAGGGTTGA+AGG 0.396529 5_2:-1702530 None:intergenic
AGGACCAATCTCGAATTAAA+GGG 0.404517 5_2:+1702718 None:intergenic
GAAGAAAGTGAGCGGGTTTG+AGG 0.425851 5_2:+1705528 None:intergenic
CTATCCCATATAGGATTTAA+GGG 0.439135 5_2:+1702824 None:intergenic
GCTAAGATTGTTTGTAGCAA+TGG 0.445005 5_2:+1705289 None:intergenic
TTCTGCTTTGACAGAACAGT+TGG 0.445567 5_2:-1702848 Msa0748460:intron
GGATACATGCTGAACTTAAA+CGG 0.451608 5_2:-1705173 Msa0748460:CDS
GAGCGGGATCTATGGTGGTT+TGG 0.455036 5_2:+1705457 None:intergenic
TCAAACCATCTATCCCATAT+AGG 0.457946 5_2:+1702815 None:intergenic
GCTGGAAAAGCTTGAGAGGA+TGG 0.463059 5_2:-1703127 Msa0748460:CDS
AAATTCAATGTAGGAAGAGC+TGG 0.464733 5_2:-1703145 Msa0748460:intron
TGGTTAGAAGTGCGTTTGTT+TGG 0.471868 5_2:+1705504 None:intergenic
CCAAAGGCTGCTGCAGATGT+TGG 0.474766 5_2:-1702778 Msa0748460:CDS
TACAATGGAAGCAATGGGTC+AGG 0.475240 5_2:+1702963 None:intergenic
ACTGAACAAAGTGGGTCGTC+GGG 0.478830 5_2:+1705229 None:intergenic
AAGAAAGTGAGCGGGTTTGA+GGG 0.479219 5_2:+1705529 None:intergenic
CAGATACAAGAGGGAAACAT+AGG 0.483669 5_2:-1705194 Msa0748460:CDS
ATATGACCGGGGAATGAGAA+TGG 0.487064 5_2:+1705391 None:intergenic
ACAAGAAACAAGGTACTTAG+AGG 0.487565 5_2:-1705145 Msa0748460:intron
AATAGCTTGATGAAGCTAAA+GGG 0.491263 5_2:+1705326 None:intergenic
AAATAGCTTGATGAAGCTAA+AGG 0.493185 5_2:+1705325 None:intergenic
TGGTCCCTTAAATCCTATAT+GGG 0.499485 5_2:-1702828 Msa0748460:CDS
TGCTTGGCAATGTCGAGAAA+AGG 0.501990 5_2:-1702986 Msa0748460:CDS
ATATGGGATAGATGGTTTGA+AGG 0.520251 5_2:-1702812 Msa0748460:CDS
GGAGGGGTGAGTATCTCAAC+TGG 0.521496 5_2:-1702554 Msa0748460:three_prime_UTR
GACTGAACAAAGTGGGTCGT+CGG 0.531551 5_2:+1705228 None:intergenic
CAGCAGCCTTTGGAATCTTG+GGG 0.533463 5_2:+1702788 None:intergenic
TGGGACCCATTCTCATTCCC+CGG 0.534066 5_2:-1705397 Msa0748460:five_prime_UTR
TTTCTTAAATGATCATCGAC+TGG 0.535411 5_2:+1702584 None:intergenic
TTAGCCTCAGAAAATGCAAT+CGG 0.537051 5_2:-1705271 Msa0748460:exon
ACATGCTGAACTTAAACGGT+TGG 0.538744 5_2:-1705169 Msa0748460:CDS
GGATTTGTAGTGTAGAATGT+GGG 0.540585 5_2:+1705550 None:intergenic
TATGACCGGGGAATGAGAAT+GGG 0.542153 5_2:+1705392 None:intergenic
GTTTGGTGAAGAAAGTGAGC+GGG 0.543461 5_2:+1705521 None:intergenic
CCTCAGAAAATGCAATCGGA+TGG 0.564403 5_2:-1705267 Msa0748460:exon
AATGAAAGAAACAACAGAGC+GGG 0.566645 5_2:+1705441 None:intergenic
AAGAGCTGGAAAAGCTTGAG+AGG 0.574159 5_2:-1703131 Msa0748460:CDS
CATTTAAGAAATGTAAATGG+AGG 0.575476 5_2:-1702572 Msa0748460:three_prime_UTR
ATTTAAGAAATGTAAATGGA+GGG 0.576481 5_2:-1702571 Msa0748460:three_prime_UTR
TTTAAGAAATGTAAATGGAG+GGG 0.587286 5_2:-1702570 Msa0748460:three_prime_UTR
CATGGTCAACACTTAATGGG+TGG 0.588204 5_2:-1705417 Msa0748460:five_prime_UTR
ATGGTCAACACTTAATGGGT+GGG 0.597116 5_2:-1705416 Msa0748460:five_prime_UTR
GGGATTTGTAGTGTAGAATG+TGG 0.597864 5_2:+1705549 None:intergenic
CTCAGAAAATGCAATCGGAT+GGG 0.599299 5_2:-1705266 Msa0748460:exon
GATTTGTAGTGTAGAATGTG+GGG 0.624298 5_2:+1705551 None:intergenic
AAGACTGAGACTGAACAAAG+TGG 0.627696 5_2:+1705220 None:intergenic
CAACAGAGCGGGATCTATGG+TGG 0.631039 5_2:+1705452 None:intergenic
GAAGGACCCCAAGATTCCAA+AGG 0.632878 5_2:-1702794 Msa0748460:CDS
AGACTGAGACTGAACAAAGT+GGG 0.647300 5_2:+1705221 None:intergenic
ATTTGTAGTGTAGAATGTGG+GGG 0.661830 5_2:+1705552 None:intergenic
TTTGTAGTGTAGAATGTGGG+GGG 0.665344 5_2:+1705553 None:intergenic
TGTTTGGTGAAGAAAGTGAG+CGG 0.672165 5_2:+1705520 None:intergenic
AACGGTTGGAACAAGAAACA+AGG 0.682214 5_2:-1705155 Msa0748460:CDS
TAATGAAAGAAACAACAGAG+CGG 0.759258 5_2:+1705440 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTCTTTTTTTAATAATT+GGG + chr5_2:1703769-1703788 None:intergenic 10.0%
!!! AAAGTTCTTTTTTTAATAAT+TGG + chr5_2:1703770-1703789 None:intergenic 10.0%
!!! AAAAAAAGAACTTTTTTTTT+TGG - chr5_2:1703776-1703795 Msa0748460:intron 10.0%
!!! AAAAAAGAACTTTTTTTTTT+GGG - chr5_2:1703777-1703796 Msa0748460:intron 10.0%
!!! TCAATTAAAGCTAAATTTTA+AGG + chr5_2:1703205-1703224 None:intergenic 15.0%
!!! TGAATTTTTTGTTTTTAGTT+TGG - chr5_2:1703250-1703269 Msa0748460:intron 15.0%
!!! AAAAAGAACTTTTTTTTTTG+GGG - chr5_2:1703778-1703797 Msa0748460:intron 15.0%
!! AAAATAATCTTAACTAGTTA+AGG + chr5_2:1704786-1704805 None:intergenic 15.0%
!! TTACAAAAGTAATTTATCAA+AGG + chr5_2:1705258-1705277 None:intergenic 15.0%
!! TTAACGAGTAATAGTAAAAT+GGG - chr5_2:1703157-1703176 Msa0748460:intron 20.0%
!!! TTTAGCTTTAATTGATTTCT+TGG - chr5_2:1703210-1703229 Msa0748460:intron 20.0%
!!! TTAGCTTTAATTGATTTCTT+GGG - chr5_2:1703211-1703230 Msa0748460:intron 20.0%
!!! TCAACTTTTGATAGTTAAAA+GGG - chr5_2:1703383-1703402 Msa0748460:intron 20.0%
!!! TTGAATTTTTAGTGACTATA+TGG - chr5_2:1703485-1703504 Msa0748460:intron 20.0%
!!! TAAAATTTTGTTGTGTTTGT+TGG + chr5_2:1704339-1704358 None:intergenic 20.0%
!! TTGATCTTATTAAAGATTCA+GGG - chr5_2:1704365-1704384 Msa0748460:intron 20.0%
!! ATTCACTATAAATTCAATGT+AGG - chr5_2:1704990-1705009 Msa0748460:intron 20.0%
!! AAATAAACTCACAAAATTTC+AGG + chr5_2:1705449-1705468 None:intergenic 20.0%
!! GATCATTTAAGAAATGTAAA+TGG - chr5_2:1705569-1705588 Msa0748460:five_prime_UTR 20.0%
!! ATTTAAGAAATGTAAATGGA+GGG - chr5_2:1705573-1705592 Msa0748460:five_prime_UTR 20.0%
! TTGAAGTGAAAAAATATGAC+CGG + chr5_2:1702769-1702788 None:intergenic 25.0%
! GTTAACGAGTAATAGTAAAA+TGG - chr5_2:1703156-1703175 Msa0748460:intron 25.0%
! ACTGAAAAGTAATGACAATT+TGG - chr5_2:1703289-1703308 Msa0748460:intron 25.0%
! CTGAAAAGTAATGACAATTT+GGG - chr5_2:1703290-1703309 Msa0748460:intron 25.0%
! TGAAAAGTAATGACAATTTG+GGG - chr5_2:1703291-1703310 Msa0748460:intron 25.0%
!!! GTCAACTTTTGATAGTTAAA+AGG - chr5_2:1703382-1703401 Msa0748460:intron 25.0%
! TTTGTAATAACAGCAACATA+AGG - chr5_2:1703925-1703944 Msa0748460:intron 25.0%
! AGAAAGAAAGACGAAAATTA+AGG + chr5_2:1703995-1704014 None:intergenic 25.0%
! CTTGATCTTATTAAAGATTC+AGG - chr5_2:1704364-1704383 Msa0748460:intron 25.0%
! GCAAATTACGAATTAATCAA+GGG + chr5_2:1704662-1704681 None:intergenic 25.0%
!!! CATTCATTTCTGAGATATAT+AGG - chr5_2:1704854-1704873 Msa0748460:intron 25.0%
!! ATCATCTTTTCTGTAAAAAG+TGG + chr5_2:1704902-1704921 None:intergenic 25.0%
! TCAAATTTGATCAGACATTA+TGG + chr5_2:1705093-1705112 None:intergenic 25.0%
! ATTAAGTTTGAATTTCTGTC+TGG - chr5_2:1705118-1705137 Msa0748460:intron 25.0%
! CATTTAAGAAATGTAAATGG+AGG - chr5_2:1705572-1705591 Msa0748460:five_prime_UTR 25.0%
! TTTAAGAAATGTAAATGGAG+GGG - chr5_2:1705574-1705593 Msa0748460:five_prime_UTR 25.0%
!!! AACTGGTTTGAAATAGTTAA+GGG - chr5_2:1705607-1705626 Msa0748460:five_prime_UTR 25.0%
TAATGAAAGAAACAACAGAG+CGG + chr5_2:1702707-1702726 None:intergenic 30.0%
!! CTGTTGTTTCTTTCATTACA+TGG - chr5_2:1702709-1702728 Msa0748460:three_prime_UTR 30.0%
ATTACATGGTCAACACTTAA+TGG - chr5_2:1702723-1702742 Msa0748460:three_prime_UTR 30.0%
TTACATGGTCAACACTTAAT+GGG - chr5_2:1702724-1702743 Msa0748460:three_prime_UTR 30.0%
TGAAGTGAAAAAATATGACC+GGG + chr5_2:1702768-1702787 None:intergenic 30.0%
AATAGCTTGATGAAGCTAAA+GGG + chr5_2:1702821-1702840 None:intergenic 30.0%
AAATAGCTTGATGAAGCTAA+AGG + chr5_2:1702822-1702841 None:intergenic 30.0%
!!! AGTCTTTTTCAGATACAAGA+GGG - chr5_2:1702941-1702960 Msa0748460:intron 30.0%
! TCTTCATCTTTACCATGTTT+TGG - chr5_2:1703081-1703100 Msa0748460:intron 30.0%
AAGAACAAAACTCCAAAACA+TGG + chr5_2:1703096-1703115 None:intergenic 30.0%
! TTCCATGTACTTCAAGTTTA+AGG - chr5_2:1703360-1703379 Msa0748460:intron 30.0%
GAACAAATATTTGCAACATG+AGG + chr5_2:1703446-1703465 None:intergenic 30.0%
!! TTCGTCTTGTTCTAACATTT+TGG + chr5_2:1703598-1703617 None:intergenic 30.0%
ACGAAAATTAAGGCGAAAAA+TGG + chr5_2:1703985-1704004 None:intergenic 30.0%
TGTGCCATCAAACAAAAAAA+TGG - chr5_2:1704180-1704199 Msa0748460:intron 30.0%
GTGCCATCAAACAAAAAAAT+GGG - chr5_2:1704181-1704200 Msa0748460:intron 30.0%
CTTGTGTAGAAAATATGGTA+AGG + chr5_2:1704281-1704300 None:intergenic 30.0%
! TACCATATTTTCTACACAAG+AGG - chr5_2:1704281-1704300 Msa0748460:intron 30.0%
GGATAGACATTTATCAGTAA+TGG + chr5_2:1704414-1704433 None:intergenic 30.0%
TCAAGGGAACTGAAAAATTA+AGG + chr5_2:1704646-1704665 None:intergenic 30.0%
GGCAAATTACGAATTAATCA+AGG + chr5_2:1704663-1704682 None:intergenic 30.0%
! ATGTCTGAAGTTGATTGATA+AGG - chr5_2:1704687-1704706 Msa0748460:intron 30.0%
ATAAGCCAGTTACCTAAAAT+TGG + chr5_2:1704716-1704735 None:intergenic 30.0%
!!! TATATAGGTTGTTTTTCCAG+CGG - chr5_2:1704869-1704888 Msa0748460:intron 30.0%
! TCTTTTCTGTAAAAAGTGGT+TGG + chr5_2:1704898-1704917 None:intergenic 30.0%
! GATTTTACAATGGAAGCAAT+GGG + chr5_2:1705189-1705208 None:intergenic 30.0%
! AGATTTTACAATGGAAGCAA+TGG + chr5_2:1705190-1705209 None:intergenic 30.0%
! TGGCATAAGAGATTTTACAA+TGG + chr5_2:1705199-1705218 None:intergenic 30.0%
TAGCATTAAACACTAGAAGT+TGG + chr5_2:1705219-1705238 None:intergenic 30.0%
CTATCCCATATAGGATTTAA+GGG + chr5_2:1705323-1705342 None:intergenic 30.0%
TCTATCCCATATAGGATTTA+AGG + chr5_2:1705324-1705343 None:intergenic 30.0%
TAAATCCTATATGGGATAGA+TGG - chr5_2:1705324-1705343 Msa0748460:five_prime_UTR 30.0%
AGGGAAAAAAACGTAGAATT+TGG + chr5_2:1705410-1705429 None:intergenic 30.0%
! TTTTCCCTTTAATTCGAGAT+TGG - chr5_2:1705422-1705441 Msa0748460:five_prime_UTR 30.0%
TTTCTTAAATGATCATCGAC+TGG + chr5_2:1705563-1705582 None:intergenic 30.0%
!! CAACTGGTTTGAAATAGTTA+AGG - chr5_2:1705606-1705625 Msa0748460:five_prime_UTR 30.0%
ATTTGTAGTGTAGAATGTGG+GGG + chr5_2:1702595-1702614 None:intergenic 35.0%
GATTTGTAGTGTAGAATGTG+GGG + chr5_2:1702596-1702615 None:intergenic 35.0%
GGATTTGTAGTGTAGAATGT+GGG + chr5_2:1702597-1702616 None:intergenic 35.0%
AATGAAAGAAACAACAGAGC+GGG + chr5_2:1702706-1702725 None:intergenic 35.0%
GAAGTGAAAAAATATGACCG+GGG + chr5_2:1702767-1702786 None:intergenic 35.0%
GCTAAGATTGTTTGTAGCAA+TGG + chr5_2:1702858-1702877 None:intergenic 35.0%
TTAGCCTCAGAAAATGCAAT+CGG - chr5_2:1702873-1702892 Msa0748460:intron 35.0%
!!! CAGTCTTTTTCAGATACAAG+AGG - chr5_2:1702940-1702959 Msa0748460:intron 35.0%
GGATACATGCTGAACTTAAA+CGG - chr5_2:1702971-1702990 Msa0748460:CDS 35.0%
! ACAAGAAACAAGGTACTTAG+AGG - chr5_2:1702999-1703018 Msa0748460:intron 35.0%
ATTTCTTGGGATACTCTTCT+GGG - chr5_2:1703224-1703243 Msa0748460:intron 35.0%
GGGAATTATGTTGTTGTGAT+AGG - chr5_2:1703311-1703330 Msa0748460:intron 35.0%
TTATGTTGTTGTGATAGGAG+AGG - chr5_2:1703316-1703335 Msa0748460:intron 35.0%
!! ATAGGAGAGGTACTTGTAAT+TGG - chr5_2:1703329-1703348 Msa0748460:intron 35.0%
!! TAGGAGAGGTACTTGTAATT+GGG - chr5_2:1703330-1703349 Msa0748460:intron 35.0%
AACTTGAAGTACATGGAAGA+TGG + chr5_2:1703358-1703377 None:intergenic 35.0%
GACCTTAAACTTGAAGTACA+TGG + chr5_2:1703365-1703384 None:intergenic 35.0%
!!! TTTTTTTTTTGGGGTAGCCA+AGG - chr5_2:1703787-1703806 Msa0748460:intron 35.0%
ACAAAGTCTCTCATAGCAAT+GGG + chr5_2:1703817-1703836 None:intergenic 35.0%
CCTCAAATTGTTCAAAAGTC+AGG + chr5_2:1704061-1704080 None:intergenic 35.0%
! CCTGACTTTTGAACAATTTG+AGG - chr5_2:1704058-1704077 Msa0748460:intron 35.0%
GTAGTTGCGTTATGCTTATT+TGG - chr5_2:1704101-1704120 Msa0748460:intron 35.0%
!!! CTGCCCATTTTTTTGTTTGA+TGG + chr5_2:1704187-1704206 None:intergenic 35.0%
AGCCTCTTGTGTAGAAAATA+TGG + chr5_2:1704286-1704305 None:intergenic 35.0%
! AGGGTCTTATGAAAGATTCA+GGG - chr5_2:1704312-1704331 Msa0748460:intron 35.0%
GGGTCTTGATAAATTAGTGT+TGG - chr5_2:1704385-1704404 Msa0748460:intron 35.0%
TCTAGATGACATGTTCTGTT+TGG + chr5_2:1704435-1704454 None:intergenic 35.0%
TTACCTGCGTTGGAATATTT+AGG - chr5_2:1704466-1704485 Msa0748460:intron 35.0%
TACCTGCGTTGGAATATTTA+GGG - chr5_2:1704467-1704486 Msa0748460:intron 35.0%
! CAAGAACTCAAAAAAGGTGT+TGG + chr5_2:1704517-1704536 None:intergenic 35.0%
GCAGAACAAGAACTCAAAAA+AGG + chr5_2:1704523-1704542 None:intergenic 35.0%
!!! TTTTGAGTTCTTGTTCTGCT+CGG - chr5_2:1704524-1704543 Msa0748460:intron 35.0%
TGTTTAAGTCAATTGCACAG+GGG + chr5_2:1704594-1704613 None:intergenic 35.0%
GTGTTTAAGTCAATTGCACA+GGG + chr5_2:1704595-1704614 None:intergenic 35.0%
! GCAATTGACTTAAACACCAA+TGG - chr5_2:1704598-1704617 Msa0748460:intron 35.0%
TATTTGCATACTGTGTCCAT+TGG + chr5_2:1704617-1704636 None:intergenic 35.0%
! TCTGAAGTTGATTGATAAGG+TGG - chr5_2:1704690-1704709 Msa0748460:intron 35.0%
! TTGATAAGGTGGCCAATTTT+AGG - chr5_2:1704701-1704720 Msa0748460:intron 35.0%
CAGATAATTAGTGCTCATGA+TGG + chr5_2:1704813-1704832 None:intergenic 35.0%
TCATGAGCACTAATTATCTG+TGG - chr5_2:1704813-1704832 Msa0748460:intron 35.0%
TTTCTGTAAAAAGTGGTTGG+AGG + chr5_2:1704895-1704914 None:intergenic 35.0%
AAATTCAATGTAGGAAGAGC+TGG - chr5_2:1704999-1705018 Msa0748460:intron 35.0%
TTGGTCCCTTAAATCCTATA+TGG - chr5_2:1705315-1705334 Msa0748460:five_prime_UTR 35.0%
TGGTCCCTTAAATCCTATAT+GGG - chr5_2:1705316-1705335 Msa0748460:five_prime_UTR 35.0%
TCAAACCATCTATCCCATAT+AGG + chr5_2:1705332-1705351 None:intergenic 35.0%
ATATGGGATAGATGGTTTGA+AGG - chr5_2:1705332-1705351 Msa0748460:five_prime_UTR 35.0%
AGGACCAATCTCGAATTAAA+GGG + chr5_2:1705429-1705448 None:intergenic 35.0%
TTTGTAGTGTAGAATGTGGG+GGG + chr5_2:1702594-1702613 None:intergenic 40.0%
GGGATTTGTAGTGTAGAATG+TGG + chr5_2:1702598-1702617 None:intergenic 40.0%
! TGTTTGGTGAAGAAAGTGAG+CGG + chr5_2:1702627-1702646 None:intergenic 40.0%
! TGGTTAGAAGTGCGTTTGTT+TGG + chr5_2:1702643-1702662 None:intergenic 40.0%
! GACAGAGAAAGAGACAGTTT+TGG + chr5_2:1702663-1702682 None:intergenic 40.0%
! ATGGTCAACACTTAATGGGT+GGG - chr5_2:1702728-1702747 Msa0748460:three_prime_UTR 40.0%
CTCAGAAAATGCAATCGGAT+GGG - chr5_2:1702878-1702897 Msa0748460:intron 40.0%
AGACTGAGACTGAACAAAGT+GGG + chr5_2:1702926-1702945 None:intergenic 40.0%
AAGACTGAGACTGAACAAAG+TGG + chr5_2:1702927-1702946 None:intergenic 40.0%
CAGATACAAGAGGGAAACAT+AGG - chr5_2:1702950-1702969 Msa0748460:intron 40.0%
ACATGCTGAACTTAAACGGT+TGG - chr5_2:1702975-1702994 Msa0748460:CDS 40.0%
AACGGTTGGAACAAGAAACA+AGG - chr5_2:1702989-1703008 Msa0748460:CDS 40.0%
! GAAACAAGGTACTTAGAGGT+TGG - chr5_2:1703003-1703022 Msa0748460:intron 40.0%
GATTTCTTGGGATACTCTTC+TGG - chr5_2:1703223-1703242 Msa0748460:intron 40.0%
!! AGGAGAGGTACTTGTAATTG+GGG - chr5_2:1703331-1703350 Msa0748460:intron 40.0%
CAAAGTCTCTCATAGCAATG+GGG + chr5_2:1703816-1703835 None:intergenic 40.0%
GACAAAGTCTCTCATAGCAA+TGG + chr5_2:1703818-1703837 None:intergenic 40.0%
GAGACTTGATGTTTGACGTT+TGG - chr5_2:1704025-1704044 Msa0748460:intron 40.0%
! GTCTCTTTTGCTCACTCTAT+AGG + chr5_2:1704133-1704152 None:intergenic 40.0%
GCACATGTTTGTATGCACAT+TGG - chr5_2:1704156-1704175 Msa0748460:intron 40.0%
GTTGCGTACAAAAGACCAAA+TGG + chr5_2:1704259-1704278 None:intergenic 40.0%
CTGAATCTTTCATAAGACCC+TGG + chr5_2:1704313-1704332 None:intergenic 40.0%
! CAGGGTCTTATGAAAGATTC+AGG - chr5_2:1704311-1704330 Msa0748460:intron 40.0%
AACCCTAAATATTCCAACGC+AGG + chr5_2:1704472-1704491 None:intergenic 40.0%
!! AACTCAAAAAAGGTGTTGGC+AGG + chr5_2:1704513-1704532 None:intergenic 40.0%
TTCTTGTTCTGCTCGGTTAA+GGG - chr5_2:1704531-1704550 Msa0748460:intron 40.0%
GGGGATTTGAGAAACATCAA+TGG + chr5_2:1704575-1704594 None:intergenic 40.0%
GGTGTTTAAGTCAATTGCAC+AGG + chr5_2:1704596-1704615 None:intergenic 40.0%
CAATCAACTTCAGACATTGC+TGG + chr5_2:1704684-1704703 None:intergenic 40.0%
! AACTGGCTTATATCAGTCGT+TGG - chr5_2:1704725-1704744 Msa0748460:intron 40.0%
TGAGCACTAATTATCTGTGG+AGG - chr5_2:1704816-1704835 Msa0748460:intron 40.0%
TTCTGCTTTGACAGAACAGT+TGG - chr5_2:1705296-1705315 Msa0748460:five_prime_UTR 40.0%
CAGGACCAATCTCGAATTAA+AGG + chr5_2:1705430-1705449 None:intergenic 40.0%
! AAGAAAGTGAGCGGGTTTGA+GGG + chr5_2:1702618-1702637 None:intergenic 45.0%
! GTTTGGTGAAGAAAGTGAGC+GGG + chr5_2:1702626-1702645 None:intergenic 45.0%
AAACAACAGAGCGGGATCTA+TGG + chr5_2:1702698-1702717 None:intergenic 45.0%
CATGGTCAACACTTAATGGG+TGG - chr5_2:1702727-1702746 Msa0748460:three_prime_UTR 45.0%
TATGACCGGGGAATGAGAAT+GGG + chr5_2:1702755-1702774 None:intergenic 45.0%
ATATGACCGGGGAATGAGAA+TGG + chr5_2:1702756-1702775 None:intergenic 45.0%
! CCATCCGATTGCATTTTCTG+AGG + chr5_2:1702880-1702899 None:intergenic 45.0%
CCTCAGAAAATGCAATCGGA+TGG - chr5_2:1702877-1702896 Msa0748460:intron 45.0%
GCTATGAGAGACTTTGTCTC+TGG - chr5_2:1703820-1703839 Msa0748460:intron 45.0%
! AGAGACTTTGTCTCTGGTTC+AGG - chr5_2:1703826-1703845 Msa0748460:intron 45.0%
! GAGACTTTGTCTCTGGTTCA+GGG - chr5_2:1703827-1703846 Msa0748460:intron 45.0%
! AGACTTTGTCTCTGGTTCAG+GGG - chr5_2:1703828-1703847 Msa0748460:intron 45.0%
! GGATGAGTTTTGCTGAGACT+TGG - chr5_2:1704079-1704098 Msa0748460:intron 45.0%
! TACACAAGAGGCTGTTTCCA+GGG - chr5_2:1704293-1704312 Msa0748460:intron 45.0%
TGATGTGACATTACCTGCGT+TGG - chr5_2:1704456-1704475 Msa0748460:intron 45.0%
GTTCTTGTTCTGCTCGGTTA+AGG - chr5_2:1704530-1704549 Msa0748460:intron 45.0%
!! GGTGGCCAATTTTAGGTAAC+TGG - chr5_2:1704708-1704727 Msa0748460:intron 45.0%
AAGAGCTGGAAAAGCTTGAG+AGG - chr5_2:1705013-1705032 Msa0748460:intron 45.0%
!!! TGTCTGGTGTTTGTTTTGCC+AGG - chr5_2:1705134-1705153 Msa0748460:intron 45.0%
!!! GTTTGTTTTGCCAGGATGCT+TGG - chr5_2:1705142-1705161 Msa0748460:intron 45.0%
TGCTTGGCAATGTCGAGAAA+AGG - chr5_2:1705158-1705177 Msa0748460:CDS 45.0%
TACAATGGAAGCAATGGGTC+AGG + chr5_2:1705184-1705203 None:intergenic 45.0%
GTAGAATGTGGGGGGTGAAA+AGG + chr5_2:1702586-1702605 None:intergenic 50.0%
! GAAGAAAGTGAGCGGGTTTG+AGG + chr5_2:1702619-1702638 None:intergenic 50.0%
AAAGTGGGTCGTCGGGTTAT+CGG + chr5_2:1702911-1702930 None:intergenic 50.0%
ACTGAACAAAGTGGGTCGTC+GGG + chr5_2:1702918-1702937 None:intergenic 50.0%
GACTGAACAAAGTGGGTCGT+CGG + chr5_2:1702919-1702938 None:intergenic 50.0%
AGTCTCTCATAGCAATGGGG+TGG + chr5_2:1703813-1703832 None:intergenic 50.0%
TGGTTCAGGGGTCTTCACTT+AGG - chr5_2:1703840-1703859 Msa0748460:intron 50.0%
!! ATGCGGGAGCATTAGTGCAA+CGG + chr5_2:1704211-1704230 None:intergenic 50.0%
! CTACACAAGAGGCTGTTTCC+AGG - chr5_2:1704292-1704311 Msa0748460:intron 50.0%
GCTGGAAAAGCTTGAGAGGA+TGG - chr5_2:1705017-1705036 Msa0748460:intron 50.0%
GAAGGACCCCAAGATTCCAA+AGG - chr5_2:1705350-1705369 Msa0748460:five_prime_UTR 50.0%
!! CAGCAGCCTTTGGAATCTTG+GGG + chr5_2:1705359-1705378 None:intergenic 50.0%
!! GCAGCAGCCTTTGGAATCTT+GGG + chr5_2:1705360-1705379 None:intergenic 50.0%
!! TGCAGCAGCCTTTGGAATCT+TGG + chr5_2:1705361-1705380 None:intergenic 50.0%
GAGCGGGATCTATGGTGGTT+TGG + chr5_2:1702690-1702709 None:intergenic 55.0%
CAACAGAGCGGGATCTATGG+TGG + chr5_2:1702695-1702714 None:intergenic 55.0%
TGGGACCCATTCTCATTCCC+CGG - chr5_2:1702747-1702766 Msa0748460:three_prime_UTR 55.0%
TCATAGCAATGGGGTGGCCT+TGG + chr5_2:1703807-1703826 None:intergenic 55.0%
!! TGCGGGAGCATTAGTGCAAC+GGG + chr5_2:1704210-1704229 None:intergenic 55.0%
ACTAATGCTCCCGCATACGC+AGG - chr5_2:1704215-1704234 Msa0748460:intron 55.0%
CTAATGCTCCCGCATACGCA+GGG - chr5_2:1704216-1704235 Msa0748460:intron 55.0%
CAAATGGTGAGACCCCTTCC+TGG + chr5_2:1704243-1704262 None:intergenic 55.0%
CAGGAAGGGGTCTCACCATT+TGG - chr5_2:1704241-1704260 Msa0748460:intron 55.0%
AAAAAGTGGTTGGAGGCCGC+TGG + chr5_2:1704888-1704907 None:intergenic 55.0%
CTCGACATTGCCAAGCATCC+TGG + chr5_2:1705155-1705174 None:intergenic 55.0%
CCAACATCTGCAGCAGCCTT+TGG + chr5_2:1705369-1705388 None:intergenic 55.0%
CCAAAGGCTGCTGCAGATGT+TGG - chr5_2:1705366-1705385 Msa0748460:five_prime_UTR 55.0%
GGAGGGGTGAGTATCTCAAC+TGG - chr5_2:1705590-1705609 Msa0748460:five_prime_UTR 55.0%
TTCCTGGACCCTGCGTATGC+GGG + chr5_2:1704227-1704246 None:intergenic 60.0%
CTTCCTGGACCCTGCGTATG+CGG + chr5_2:1704228-1704247 None:intergenic 60.0%
GCATACGCAGGGTCCAGGAA+GGG - chr5_2:1704227-1704246 Msa0748460:intron 60.0%
CATACGCAGGGTCCAGGAAG+GGG - chr5_2:1704228-1704247 Msa0748460:intron 60.0%
CGCATACGCAGGGTCCAGGA+AGG - chr5_2:1704226-1704245 Msa0748460:intron 65.0%
CTCCCGCATACGCAGGGTCC+AGG - chr5_2:1704222-1704241 Msa0748460:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 1702533 1705633 1702533 ID=Msa0748460;Name=Msa0748460
chr5_2 mRNA 1702533 1705633 1702533 ID=Msa0748460-mRNA-1;Parent=Msa0748460;Name=Msa0748460-mRNA-1;_AED=0.03;_eAED=0.03;_QI=353|1|1|1|0.66|0.75|4|236|106
chr5_2 exon 1702533 1702856 1702533 ID=Msa0748460-mRNA-1:exon:509;Parent=Msa0748460-mRNA-1
chr5_2 exon 1702966 1703010 1702966 ID=Msa0748460-mRNA-1:exon:508;Parent=Msa0748460-mRNA-1
chr5_2 exon 1703102 1703154 1703102 ID=Msa0748460-mRNA-1:exon:507;Parent=Msa0748460-mRNA-1
chr5_2 exon 1705146 1705633 1705146 ID=Msa0748460-mRNA-1:exon:506;Parent=Msa0748460-mRNA-1
chr5_2 five_prime_UTR 1705281 1705633 1705281 ID=Msa0748460-mRNA-1:five_prime_utr;Parent=Msa0748460-mRNA-1
chr5_2 CDS 1705146 1705280 1705146 ID=Msa0748460-mRNA-1:cds;Parent=Msa0748460-mRNA-1
chr5_2 CDS 1703102 1703154 1703102 ID=Msa0748460-mRNA-1:cds;Parent=Msa0748460-mRNA-1
chr5_2 CDS 1702966 1703010 1702966 ID=Msa0748460-mRNA-1:cds;Parent=Msa0748460-mRNA-1
chr5_2 CDS 1702769 1702856 1702769 ID=Msa0748460-mRNA-1:cds;Parent=Msa0748460-mRNA-1
chr5_2 three_prime_UTR 1702533 1702768 1702533 ID=Msa0748460-mRNA-1:three_prime_utr;Parent=Msa0748460-mRNA-1
Gene Sequence

>Msa0748460

ATGCAATCGGATGGGTCTGAATCCGATAACCCGACGACCCACTTTGTTCAGTCTCAGTCTTTTTCAGATACAAGAGGGAAACATAGGATACATGCTGAACTTAAACGGTTGGAACAAGAAACAAGGTACTTAGAGGAAGAGCTGGAAAAGCTTGAGAGGATGGACAAAGCATCTGCATCGTGTAAAGAGATGCTTGGCAATGTCGAGAAAAGGCCTGACCCATTGCTTCCATTAACAGTTGGTCCCTTAAATCCTATATGGGATAGATGGTTTGAAGGACCCCAAGATTCCAAAGGCTGCTGCAGATGTTGGATTCTCTGA
Protein sequence

>Msa0748460

MQSDGSESDNPTTHFVQSQSFSDTRGKHRIHAELKRLEQETRYLEEELEKLERMDKASASCKEMLGNVEKRPDPLLPLTVGPLNPIWDRWFEGPQDSKGCCRCWIL*