AlfalfaGEDB | Msa0078000

M. sativa cultivar zhongmu-4 / Msa0078000

KEGG: K07973
GO: -
PFAM: PF00631.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0078000	sp\|Q9FDX9\|GG1_ARATH	56.842	95	40	1	12	106	5	98	2.42e-31	109

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0078000	A0A396JVQ5	95.413	109	2	1	1	106	1	109	3.44e-70	214

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0078000	0.816216	6.199041e-52	-8.615850e-47
Msa0032840	Msa0078000	0.812240	4.682923e-51	-8.615850e-47
Msa0078000	Msa0143080	-0.829100	6.219384e-55	-8.615850e-47
Msa0078000	Msa0143240	0.801249	9.843711e-49	-8.615850e-47
Msa0078000	Msa0168640	0.813777	2.155973e-51	-8.615850e-47
Msa0078000	Msa0170560	0.943245	1.702907e-102	-8.615850e-47
Msa0078000	Msa0172960	0.801118	1.047021e-48	-8.615850e-47
Msa0078000	Msa0188680	0.809963	1.459128e-50	-8.615850e-47
Msa0078000	Msa0194970	-0.821260	4.437094e-53	-8.615850e-47
Msa0078000	Msa0230550	0.809847	1.545364e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0078000	MtrunA17_Chr1g0188131	95.413	109	2	1	1	106	1	109	6.63e-74	214
Msa0078000	MtrunA17_Chr1g0188161	79.817	109	18	3	1	106	1	108	1.63e-48	150
Msa0078000	MtrunA17_Chr1g0184941	53.846	104	47	1	3	106	7	109	7.01e-37	120
Msa0078000	MtrunA17_Chr1g0148341	40.217	92	52	2	16	105	35	125	4.77e-18	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0078000	AT3G63420.1	56.842	95	40	1	12	106	5	98	2.46e-32	109
Msa0078000	AT3G63420.2	56.842	95	40	1	12	106	5	98	2.46e-32	109
Msa0078000	AT3G22942.1	70.238	84	24	1	23	106	18	100	7.20e-32	108
Msa0078000	AT3G63420.3	56.842	95	40	1	12	106	45	138	1.23e-31	108

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Find 70 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACAGAGCGGAATCTA+TGG	0.377414	1_2:-55221003	None:intergenic
AAAGTGGGTCGTCGGGTTAT+CGG	0.364117	1_2:-55221216	None:intergenic
AAATAAACTCACAAAATTTC+AGG	0.285103	1_2:-55223741	None:intergenic
AAATAGCTTGATGAAGCTAA+AGG	0.493185	1_2:-55221127	None:intergenic
AAATTCAATGTAGGAAGAGC+TGG	0.464733	1_2:+55223296	Msa0078000:intron
AACGGTTGGAACAAGAAACA+AGG	0.682214	1_2:+55221297	Msa0078000:CDS
AACTGGTTTGAAATAGTTAA+GGG	0.382241	1_2:+55223902	Msa0078000:three_prime_UTR
AAGAAAGTGAGCGGGTTTGA+GGG	0.479219	1_2:-55220923	None:intergenic
AAGACTGAGACTGAACAAAG+TGG	0.627696	1_2:-55221232	None:intergenic
AAGAGCTGGAAAAGCTTGAG+AGG	0.574159	1_2:+55223310	Msa0078000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGAAAGAAACAACAGAG+CGG	-	chr1_2:55221015-55221034	None:intergenic	30.0%
ATTACATGGTCAACACTTAA+TGG	+	chr1_2:55221031-55221050	Msa0078000:five_prime_UTR	30.0%
TTACATGGTCAACACTTAAT+GGG	+	chr1_2:55221032-55221051	Msa0078000:five_prime_UTR	30.0%
TGAAGTGAAAAAATATGACC+GGG	-	chr1_2:55221076-55221095	None:intergenic	30.0%
AATAGCTTGATGAAGCTAAA+GGG	-	chr1_2:55221129-55221148	None:intergenic	30.0%
AAATAGCTTGATGAAGCTAA+AGG	-	chr1_2:55221130-55221149	None:intergenic	30.0%
AAGAACAAAACTCCAAAACA+TGG	-	chr1_2:55221368-55221387	None:intergenic	30.0%
TAAACACACACTATTAGAGT+AGG	+	chr1_2:55221977-55221996	Msa0078000:intron	30.0%
ACGAAAATTAAGGCGAAAAA+TGG	-	chr1_2:55222275-55222294	None:intergenic	30.0%
TGTGCCATCAAACAAAAAAA+TGG	+	chr1_2:55222470-55222489	Msa0078000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	55220841	55223928	55220841	ID=Msa0078000;Name=Msa0078000
chr1_2	mRNA	55220841	55223928	55220841	ID=Msa0078000-mRNA-1;Parent=Msa0078000;Name=Msa0078000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=353\|1\|1\|1\|1\|1\|4\|236\|106
chr1_2	exon	55220841	55221328	55220841	ID=Msa0078000-mRNA-1:exon:17186;Parent=Msa0078000-mRNA-1
chr1_2	exon	55223309	55223361	55223309	ID=Msa0078000-mRNA-1:exon:17187;Parent=Msa0078000-mRNA-1
chr1_2	exon	55223451	55223495	55223451	ID=Msa0078000-mRNA-1:exon:17188;Parent=Msa0078000-mRNA-1
chr1_2	exon	55223605	55223928	55223605	ID=Msa0078000-mRNA-1:exon:17189;Parent=Msa0078000-mRNA-1
chr1_2	five_prime_UTR	55220841	55221193	55220841	ID=Msa0078000-mRNA-1:five_prime_utr;Parent=Msa0078000-mRNA-1
chr1_2	CDS	55221194	55221328	55221194	ID=Msa0078000-mRNA-1:cds;Parent=Msa0078000-mRNA-1
chr1_2	CDS	55223309	55223361	55223309	ID=Msa0078000-mRNA-1:cds;Parent=Msa0078000-mRNA-1
chr1_2	CDS	55223451	55223495	55223451	ID=Msa0078000-mRNA-1:cds;Parent=Msa0078000-mRNA-1
chr1_2	CDS	55223605	55223692	55223605	ID=Msa0078000-mRNA-1:cds;Parent=Msa0078000-mRNA-1
chr1_2	three_prime_UTR	55223693	55223928	55223693	ID=Msa0078000-mRNA-1:three_prime_utr;Parent=Msa0078000-mRNA-1

Gene Sequence	>Msa0078000 ATGCAATCGGATGGGTCTGAATCCGATAACCCGACGACCCACTTTGTTCAGTCTCAGTCTTTTTCAGATACAAGAGGGAAACATAGGATACATGCTGAACTTAAACGGTTGGAACAAGAAACAAGGTACTTAGAGGAAGAGCTGGAAAAGCTTGAGAGGATGGACAAAGCATCTGCATCGTGTAAAGAGATGCTTGGCAATGTCGAGAAAAGGCCTGACCCATTACTTCCATTAACAGTTGGTCCCTTAAATCCTATATGGGATAGATGGTTTGAAGGACCCCAAGATTCCAAAGGCTGCTGCAGATGTTGGATTCTCTGA
Protein sequence	>Msa0078000 MQSDGSESDNPTTHFVQSQSFSDTRGKHRIHAELKRLEQETRYLEEELEKLERMDKASASCKEMLGNVEKRPDPLLPLTVGPLNPIWDRWFEGPQDSKGCCRCWIL*