AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0755460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755460 MtrunA17_Chr5g0400311 96.979 629 19 0 1 629 1 629 0.0 1261
Msa0755460 MtrunA17_Chr4g0054951 66.035 633 141 10 1 625 1 567 0.0 802
Msa0755460 MtrunA17_Chr4g0054941 62.480 629 165 11 5 625 2 567 0.0 743
Msa0755460 MtrunA17_Chr6g0466801 41.121 642 319 12 1 613 1 612 4.86e-151 450
Msa0755460 MtrunA17_Chr1g0201271 40.000 650 316 15 6 620 11 621 7.81e-142 426
Msa0755460 MtrunA17_Chr7g0260081 50.923 271 130 1 362 629 283 553 5.60e-88 284
Msa0755460 MtrunA17_Chr7g0260081 35.789 190 105 3 7 195 8 181 2.40e-29 122
Msa0755460 MtrunA17_Chr1g0205121 47.249 309 147 4 333 626 269 576 7.29e-87 282
Msa0755460 MtrunA17_Chr1g0205121 34.021 194 112 5 1 194 1 178 1.83e-26 114
Msa0755460 MtrunA17_Chr5g0394901 49.643 280 136 3 350 626 338 615 4.72e-86 281
Msa0755460 MtrunA17_Chr5g0394901 46.226 106 55 1 89 194 77 180 1.76e-23 105
Msa0755460 MtrunA17_Chr1g0185281 50.376 266 129 1 364 626 285 550 7.32e-84 273
Msa0755460 MtrunA17_Chr1g0185281 38.342 193 91 5 7 193 7 177 3.59e-31 128
Msa0755460 MtrunA17_Chr7g0263111 49.808 261 115 2 366 616 170 424 4.84e-82 266
Msa0755460 MtrunA17_Chr7g0263111 34.091 176 97 5 13 185 11 170 5.00e-25 108
Msa0755460 MtrunA17_Chr8g0355021 47.059 289 130 6 330 610 201 474 1.60e-80 263
Msa0755460 MtrunA17_Chr8g0355021 50.667 75 37 0 108 182 64 138 1.85e-16 82.4
Msa0755460 MtrunA17_Chr8g0382571 46.468 269 140 2 358 622 204 472 3.35e-77 253
Msa0755460 MtrunA17_Chr8g0382571 52.778 72 34 0 104 175 37 108 1.20e-18 89.4
Msa0755460 MtrunA17_Chr8g0375751 44.966 298 161 2 317 611 95 392 6.13e-76 248
Msa0755460 MtrunA17_Chr8g0375751 45.882 85 45 1 93 176 47 131 3.55e-16 81.3
Msa0755460 MtrunA17_Chr1g0165011 52.586 232 107 1 370 598 180 411 4.68e-75 247
Msa0755460 MtrunA17_Chr1g0165011 30.636 173 80 4 7 178 4 137 5.03e-16 80.9
Msa0755460 MtrunA17_Chr6g0452181 49.583 240 114 5 366 598 162 401 4.99e-74 244
Msa0755460 MtrunA17_Chr6g0452181 44.156 77 41 1 101 175 51 127 1.95e-15 79.3
Msa0755460 MtrunA17_Chr6g0479511 46.087 230 123 1 370 598 155 384 3.18e-71 235
Msa0755460 MtrunA17_Chr6g0479511 32.800 125 81 2 86 209 28 150 4.47e-15 77.4
Msa0755460 MtrunA17_Chr1g0189231 32.472 271 142 9 367 600 608 874 1.53e-31 131
Msa0755460 MtrunA17_Chr1g0154491 28.873 284 174 13 330 598 65 335 3.56e-20 92.4
Msa0755460 MtrunA17_Chr6g0488541 29.956 227 126 8 400 606 740 953 1.21e-16 84.3
Msa0755460 MtrunA17_Chr6g0488541 33.696 92 59 1 367 458 643 732 1.31e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0755460 AT4G18010.1 55.438 662 233 11 1 619 1 643 0.0 676
Msa0755460 AT4G18010.2 53.667 600 218 10 1 559 1 581 0.0 585
Msa0755460 AT1G34120.2 40.842 546 258 10 84 611 83 581 4.67e-135 408
Msa0755460 AT1G34120.1 38.065 620 297 13 10 611 27 577 1.81e-134 406
Msa0755460 AT1G34120.3 40.842 546 257 11 84 611 83 580 2.99e-133 403
Msa0755460 AT2G32010.2 36.190 630 323 14 17 626 17 587 2.48e-118 365
Msa0755460 AT2G32010.1 36.190 630 323 14 17 626 17 587 2.48e-118 365
Msa0755460 AT2G32010.4 36.190 630 323 14 17 626 17 587 2.48e-118 365
Msa0755460 AT2G32010.3 36.190 630 323 14 17 626 17 587 2.48e-118 365
Msa0755460 AT1G34120.5 56.680 247 103 2 367 611 182 426 3.43e-98 307
Msa0755460 AT1G71710.1 42.192 365 162 6 253 613 333 652 4.10e-88 288
Msa0755460 AT1G71710.1 40.465 215 97 3 1 193 10 215 1.40e-39 154
Msa0755460 AT1G71710.2 42.192 365 162 6 253 613 324 643 4.51e-88 288
Msa0755460 AT1G71710.2 40.465 215 97 3 1 193 1 206 1.38e-39 154
Msa0755460 AT1G05470.2 49.811 265 129 2 365 626 347 610 1.87e-85 280
Msa0755460 AT1G05470.2 32.990 194 116 2 1 194 1 180 7.27e-30 125
Msa0755460 AT1G05470.1 49.811 265 129 2 365 626 347 610 1.87e-85 280
Msa0755460 AT1G05470.1 32.990 194 116 2 1 194 1 180 7.27e-30 125
Msa0755460 AT3G63240.2 50.909 275 132 2 355 626 266 540 3.23e-85 277
Msa0755460 AT3G63240.2 35.545 211 108 4 7 214 7 192 8.10e-29 121
Msa0755460 AT3G63240.1 50.909 275 132 2 355 626 266 540 3.23e-85 277
Msa0755460 AT3G63240.1 35.545 211 108 4 7 214 7 192 8.10e-29 121
Msa0755460 AT2G37440.2 56.034 232 101 1 367 598 113 343 8.35e-83 266
Msa0755460 AT2G37440.2 39.815 108 39 2 110 191 3 110 6.04e-17 84.0
Msa0755460 AT2G37440.1 56.034 232 101 1 367 598 194 424 3.76e-82 267
Msa0755460 AT2G37440.1 32.418 182 100 4 8 188 6 165 4.03e-24 106
Msa0755460 AT2G37440.3 56.034 232 101 1 367 598 194 424 3.76e-82 267
Msa0755460 AT2G37440.3 32.418 182 100 4 8 188 6 165 4.03e-24 106
Msa0755460 AT1G34120.4 33.529 510 254 12 10 501 27 469 4.30e-82 266
Msa0755460 AT5G04980.4 48.583 247 123 2 358 600 148 394 1.11e-79 258
Msa0755460 AT5G65090.2 50.403 248 120 1 354 598 186 433 3.03e-79 259
Msa0755460 AT5G65090.2 51.316 76 37 0 104 179 8 83 2.30e-19 92.0
Msa0755460 AT2G01900.1 50.000 266 127 2 348 612 138 398 4.87e-79 257
Msa0755460 AT2G01900.1 46.667 75 39 1 107 180 59 133 1.39e-14 76.6
Msa0755460 AT5G04980.1 48.583 247 123 2 358 600 183 429 5.38e-79 257
Msa0755460 AT5G04980.1 46.269 67 36 0 104 170 7 73 1.28e-13 73.9
Msa0755460 AT5G04980.3 48.583 247 123 2 358 600 183 429 5.38e-79 257
Msa0755460 AT5G04980.3 46.269 67 36 0 104 170 7 73 1.28e-13 73.9
Msa0755460 AT2G01900.3 50.000 266 127 2 348 612 146 406 6.38e-79 257
Msa0755460 AT2G01900.3 46.667 75 39 1 107 180 67 141 1.30e-14 77.0
Msa0755460 AT5G04980.2 48.583 247 123 2 358 600 212 458 8.98e-79 258
Msa0755460 AT5G04980.2 46.269 67 36 0 104 170 36 102 1.20e-13 73.9
Msa0755460 AT5G65090.3 50.403 248 120 1 354 598 249 496 1.36e-78 259
Msa0755460 AT5G65090.3 51.316 76 37 0 104 179 71 146 3.28e-19 92.0
Msa0755460 AT5G65090.1 50.403 248 120 1 354 598 249 496 1.36e-78 259
Msa0755460 AT5G65090.1 51.316 76 37 0 104 179 71 146 3.28e-19 92.0
Msa0755460 AT2G01900.2 48.872 266 121 3 348 612 138 389 9.55e-75 245
Msa0755460 AT2G01900.2 46.667 75 39 1 107 180 59 133 1.43e-14 76.6
Msa0755460 AT1G05630.2 32.394 284 146 8 367 608 652 931 6.01e-35 142
Msa0755460 AT1G05630.3 35.398 226 115 5 367 565 652 873 1.55e-34 140
Msa0755460 AT2G43900.1 30.986 284 149 8 365 606 656 934 2.31e-33 137
Msa0755460 AT2G31830.1 32.117 274 146 8 367 609 661 925 2.43e-33 137
Msa0755460 AT2G43900.2 30.986 284 149 8 365 606 656 934 2.45e-33 137
Msa0755460 AT2G31830.2 32.117 274 146 8 367 609 661 925 3.58e-33 137
Msa0755460 AT1G65580.2 30.035 283 150 7 367 606 353 630 4.79e-33 135
Msa0755460 AT1G65580.1 30.035 283 150 7 367 606 619 896 4.80e-33 136
Msa0755460 AT2G31830.3 34.071 226 118 5 367 565 661 882 1.97e-32 134
Msa0755460 AT1G05630.1 28.931 318 146 8 367 608 652 965 1.55e-30 128
Msa0755460 AT1G47510.4 31.633 196 110 8 403 590 84 263 1.46e-18 86.7
Msa0755460 AT1G47510.1 31.633 196 110 8 403 590 146 325 6.42e-18 85.9
Msa0755460 AT1G47510.2 36.134 119 64 4 479 590 149 262 1.07e-12 69.3

Find 128 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGATTGCTTCCCATTTC+TGG 0.077647 5_2:-9250254 None:intergenic
TCATCACTAATTTGCATTCT+TGG 0.178641 5_2:-9249416 None:intergenic
CCTGTTGGAGTTGGTCTTAT+GGG 0.192645 5_2:+9250960 Msa0755460:CDS
ACCTGTTGGAGTTGGTCTTA+TGG 0.206704 5_2:+9250959 Msa0755460:CDS
GAACAATTATTATTCAATTT+TGG 0.217826 5_2:+9252794 Msa0755460:three_prime_UTR
GTTTGTTCTCATCTGACTTC+TGG 0.253654 5_2:+9251364 Msa0755460:CDS
AACGAGAATGCTTTCATATA+TGG 0.255304 5_2:+9250633 Msa0755460:CDS
TTGTCCAAAGATAAAATAAC+TGG 0.268124 5_2:-9252838 None:intergenic
TGAGTCCCTTGTGTTCTAAA+TGG 0.278238 5_2:-9249443 None:intergenic
AGATTCGGAGGTTCGGAAAT+TGG 0.279202 5_2:+9251648 Msa0755460:CDS
GCATCATAGTTCCGGAGATT+TGG 0.281629 5_2:+9250686 Msa0755460:CDS
GTCAAAGAATATAATATTAT+AGG 0.298464 5_2:+9252734 Msa0755460:three_prime_UTR
CATCATAGTTCCGGAGATTT+GGG 0.307084 5_2:+9250687 Msa0755460:CDS
TTGCATGTGAGGAAAGTTTA+TGG 0.308983 5_2:+9250498 Msa0755460:CDS
TCTTGTGCCTCACAGTTTCC+AGG 0.330792 5_2:+9250176 Msa0755460:intron
GTAAATATCACTTGGTTCTT+CGG 0.331532 5_2:-9249878 None:intergenic
CATTCAGTGGAACTACTTCC+TGG 0.351079 5_2:-9250194 None:intergenic
TCTAACGAATCAACTACTTT+AGG 0.360222 5_2:-9250738 None:intergenic
TTTATGGTATTGATCTTGAT+TGG 0.371531 5_2:+9250514 Msa0755460:CDS
TTAAGTAGTTCAGCTAGAAT+TGG 0.375907 5_2:+9250600 Msa0755460:CDS
ATATTCTCAACTCAAATATC+AGG 0.376314 5_2:+9249470 Msa0755460:CDS
ATCTTTGGACAATAAACGTC+AGG 0.377848 5_2:+9252849 Msa0755460:three_prime_UTR
TGGGAATCTATGTATCTGTT+TGG 0.377949 5_2:+9250892 Msa0755460:CDS
GAAAGTAACATAATTAGTAT+AGG 0.383320 5_2:+9250468 Msa0755460:CDS
ACCAAGAAAATTGAACTAAC+TGG 0.385102 5_2:-9252479 None:intergenic
CGCTTAATCCATCAGCATCT+TGG 0.386530 5_2:-9250821 None:intergenic
TTCATATATGGTGGTGGTGG+TGG 0.386916 5_2:+9250645 Msa0755460:CDS
ATTTGCATTCTTGGTTGCTT+AGG 0.387840 5_2:-9249407 None:intergenic
TCAGGGTAGCAATTGGATCT+TGG 0.401753 5_2:+9249800 Msa0755460:intron
TGAGTCAGTTACGTTACATA+AGG 0.402055 5_2:+9252667 Msa0755460:three_prime_UTR
AAACGTTCGCATCATAGTTC+CGG 0.406803 5_2:+9250678 Msa0755460:CDS
ATAATACACCTATCCAGAAA+TGG 0.408581 5_2:+9250241 Msa0755460:CDS
AAAGTAACATAATTAGTATA+GGG 0.408651 5_2:+9250469 Msa0755460:CDS
CCATCAGCATCTTGGTTCTC+AGG 0.413769 5_2:-9250813 None:intergenic
CGTGTTGGGCATGTATTTGA+TGG 0.413909 5_2:+9252179 Msa0755460:CDS
GAGTCAGTTACGTTACATAA+GGG 0.416582 5_2:+9252668 Msa0755460:three_prime_UTR
CATTGCATCTAACGGACGTT+CGG 0.421202 5_2:-9250538 None:intergenic
ATTGGATCTTGGAATGTTGC+TGG 0.427032 5_2:+9249811 Msa0755460:CDS
TTAATTTCATATTTGTAAGT+TGG 0.431471 5_2:-9252230 None:intergenic
TCACTAAAATCATTCACCTT+AGG 0.436230 5_2:-9249240 None:intergenic
AATCACATGCGAGGTATGTT+CGG 0.440250 5_2:+9250850 Msa0755460:CDS
CCCATAAGACCAACTCCAAC+AGG 0.442442 5_2:-9250960 None:intergenic
AGTTGGTCTTATGGGCTACA+TGG 0.444671 5_2:+9250968 Msa0755460:CDS
CCTTCATTGTTTCAAGTCAA+AGG 0.449963 5_2:-9249073 None:intergenic
CGAATATTGTGGCTAGGAAA+AGG 0.450823 5_2:+9252404 Msa0755460:CDS
ATTAATTCAGATAGATATGT+TGG 0.451212 5_2:+9252248 Msa0755460:CDS
GGGAATCTATGTATCTGTTT+GGG 0.451736 5_2:+9250893 Msa0755460:CDS
CTGAATGCTGGAAATGTACT+AGG 0.452408 5_2:+9250210 Msa0755460:CDS
TAAGCAATGAATTGCGTGTT+GGG 0.453778 5_2:+9252165 Msa0755460:CDS
TCGAATCAGTATGTTAGATT+CGG 0.454114 5_2:+9251633 Msa0755460:CDS
GTATTTGATGGATGGAAAGA+AGG 0.466341 5_2:+9252191 Msa0755460:CDS
ATTGCTTCCCATTTCTGGAT+AGG 0.468019 5_2:-9250249 None:intergenic
CCACAGCGCGCCACCTTCGC+CGG 0.468038 5_2:+9250323 Msa0755460:CDS
TCATCTGACTTCTGGTACAA+AGG 0.470086 5_2:+9251372 Msa0755460:CDS
TCATCATTCAGCATATCAAT+TGG 0.473256 5_2:-9250402 None:intergenic
GTATGTTAGATTCGGAGGTT+CGG 0.473895 5_2:+9251641 Msa0755460:CDS
AAAGTTTCACCTGTTGGAGT+TGG 0.475998 5_2:+9250951 Msa0755460:CDS
ATTGCATCTAACGGACGTTC+GGG 0.485205 5_2:-9250537 None:intergenic
AATTTGAAAGTTTCACCTGT+TGG 0.489228 5_2:+9250945 Msa0755460:CDS
TAATACACCTATCCAGAAAT+GGG 0.489685 5_2:+9250242 Msa0755460:CDS
TGAACTAATGAATTATGATC+AGG 0.491564 5_2:+9251690 Msa0755460:CDS
GAGAACAAACAAAGCACATA+CGG 0.492200 5_2:-9251351 None:intergenic
CCTTTGACTTGAAACAATGA+AGG 0.495124 5_2:+9249073 Msa0755460:exon
ATTGCAATTAATTACCATGC+AGG 0.498674 5_2:-9252305 None:intergenic
TTGGTGAGATTCCAAAAGAA+GGG 0.500872 5_2:+9252267 Msa0755460:CDS
CAATAACGAGCCCAAATCTC+CGG 0.506916 5_2:-9250697 None:intergenic
AAGATAAATTAATTGAACAT+TGG 0.507885 5_2:+9252613 Msa0755460:three_prime_UTR
GATTTGGGCTCGTTATTGAA+AGG 0.508310 5_2:+9250702 Msa0755460:CDS
CTGACTTCTGGTACAAAGGA+TGG 0.510046 5_2:+9251376 Msa0755460:CDS
TGCTCAGTATATAAATACTA+AGG 0.510156 5_2:+9249662 Msa0755460:CDS
GAAGTAGTTCCACTGAATGC+TGG 0.510491 5_2:+9250198 Msa0755460:CDS
CCGGCGAAGGTGGCGCGCTG+TGG 0.516401 5_2:-9250323 None:intergenic
ATGGCTTAATATTAAGCCTA+AGG 0.517224 5_2:+9249224 Msa0755460:CDS
GTTGGTGAGATTCCAAAAGA+AGG 0.521146 5_2:+9252266 Msa0755460:CDS
TCGGATTATCAGCAAGCAAA+TGG 0.527568 5_2:+9250869 Msa0755460:CDS
CTAAGCAATGAATTGCGTGT+TGG 0.528172 5_2:+9252164 Msa0755460:CDS
AGGATCTTGATATTGATGAC+TGG 0.535310 5_2:+9249848 Msa0755460:CDS
CTTAATACTCTCCGCAACTT+CGG 0.537866 5_2:-9250582 None:intergenic
ATTTGGGCTCGTTATTGAAA+GGG 0.541180 5_2:+9250703 Msa0755460:CDS
AATAAAGCTCACTCGTGCGA+TGG 0.543502 5_2:-9251490 None:intergenic
ATAAAGCTCACTCGTGCGAT+GGG 0.545204 5_2:-9251489 None:intergenic
GTTCTCAACACCGGCGAAGG+TGG 0.554746 5_2:-9250333 None:intergenic
TTTGAATGATGATGATGAGA+TGG 0.559090 5_2:-9248889 None:intergenic
ATGTATCTGTTTGGGTGCAG+AGG 0.559123 5_2:+9250901 Msa0755460:CDS
GCTTTCATATATGGTGGTGG+TGG 0.560950 5_2:+9250642 Msa0755460:CDS
TATGCTGAATGATGAGCTCA+TGG 0.564953 5_2:+9250410 Msa0755460:CDS
TTGGGCATGTATTTGATGGA+TGG 0.569025 5_2:+9252183 Msa0755460:CDS
AATCAGTATGTTAGATTCGG+AGG 0.570836 5_2:+9251636 Msa0755460:CDS
TTGATTCAAATCCGAAGTTG+CGG 0.571082 5_2:+9250571 Msa0755460:CDS
CCTGAGAACCAAGATGCTGA+TGG 0.572915 5_2:+9250813 Msa0755460:CDS
AGTCAGTTACGTTACATAAG+GGG 0.575484 5_2:+9252669 Msa0755460:three_prime_UTR
GAAGTTCTCAACACCGGCGA+AGG 0.576277 5_2:-9250336 None:intergenic
GAAACAATGAAGGGAAGAAG+AGG 0.580298 5_2:+9249083 Msa0755460:exon
TATCTGTTTGGGTGCAGAGG+AGG 0.583884 5_2:+9250904 Msa0755460:CDS
CTTTGACTTGAAACAATGAA+GGG 0.589740 5_2:+9249074 Msa0755460:exon
AGTACATTTCCAGCATTCAG+TGG 0.590823 5_2:-9250207 None:intergenic
GATTATCAGCAAGCAAATGG+TGG 0.594141 5_2:+9250872 Msa0755460:CDS
GTTGGTCTTATGGGCTACAT+GGG 0.595449 5_2:+9250969 Msa0755460:CDS
AGAACAAACAAAGCACATAC+GGG 0.598184 5_2:-9251350 None:intergenic
TATTTGATGGATGGAAAGAA+GGG 0.598578 5_2:+9252192 Msa0755460:CDS
ATTTGCGACATTGCATCTAA+CGG 0.600034 5_2:-9250546 None:intergenic
TGTGATCGAATATTGTGGCT+AGG 0.603815 5_2:+9252398 Msa0755460:CDS
TCTTTGGACAATAAACGTCA+GGG 0.606513 5_2:+9252850 Msa0755460:three_prime_UTR
TAGGGAATGATTTGCATGTG+AGG 0.611783 5_2:+9250487 Msa0755460:CDS
TACAAAACAAGACACAGAAG+AGG 0.617623 5_2:+9249621 Msa0755460:CDS
TATGGGCTACATGGGAAACA+AGG 0.618111 5_2:+9250977 Msa0755460:CDS
ATTATCAGCAAGCAAATGGT+GGG 0.619168 5_2:+9250873 Msa0755460:CDS
GAGAATGCTTTCATATATGG+TGG 0.620832 5_2:+9250636 Msa0755460:CDS
TTCGGAGGTTCGGAAATTGG+TGG 0.621782 5_2:+9251651 Msa0755460:CDS
TCAATATCAAGATCCTCAGA+TGG 0.622649 5_2:-9249841 None:intergenic
TCACAACCGCAACTTGCACA+AGG 0.628072 5_2:-9248801 None:intergenic
TGGACAATAAACGTCAGGGT+GGG 0.629831 5_2:+9252854 Msa0755460:three_prime_UTR
ATCTGAGATGTTATCTGACG+AGG 0.640902 5_2:+9250767 Msa0755460:CDS
TGGAAATGTACTAGGAGCAG+AGG 0.643675 5_2:+9250218 Msa0755460:CDS
GTCAGTTACGTTACATAAGG+GGG 0.646220 5_2:+9252670 Msa0755460:three_prime_UTR
AATGCTTTCATATATGGTGG+TGG 0.651242 5_2:+9250639 Msa0755460:CDS
GCAGAAGAAGTTCTCAACAC+CGG 0.655380 5_2:-9250342 None:intergenic
TGGTGAGATTCCAAAAGAAG+GGG 0.655564 5_2:+9252268 Msa0755460:CDS
GATAGTCCATTTAGAACACA+AGG 0.664992 5_2:+9249437 Msa0755460:CDS
GAAACTGAGAGTGAAGATGA+TGG 0.668913 5_2:+9249279 Msa0755460:CDS
TGCTGGAAGACATCCATCTG+AGG 0.669046 5_2:+9249828 Msa0755460:CDS
ATATAAATACTAAGGAAGTG+AGG 0.671109 5_2:+9249670 Msa0755460:CDS
TGTCCAAAGATAAAATAACT+GGG 0.678215 5_2:-9252837 None:intergenic
GCGCGACGAAATCACATGCG+AGG 0.683866 5_2:+9250841 Msa0755460:CDS
TCTGAGATGTTATCTGACGA+GGG 0.685913 5_2:+9250768 Msa0755460:CDS
TTGGACAATAAACGTCAGGG+TGG 0.688024 5_2:+9252853 Msa0755460:three_prime_UTR
GCAGGTGTGATCGAATATTG+TGG 0.699199 5_2:+9252393 Msa0755460:intron
ATAGTCCATTTAGAACACAA+GGG 0.775380 5_2:+9249438 Msa0755460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATTAATTATATAATCT+AGG - chr5_2:9252122-9252141 None:intergenic 10.0%
!! TTATAAACGTACAAAAAAAT+GGG - chr5_2:9248919-9248938 None:intergenic 15.0%
!! ATTATAAACGTACAAAAAAA+TGG - chr5_2:9248920-9248939 None:intergenic 15.0%
!! ATAATGATAAATCATAACTT+TGG + chr5_2:9249770-9249789 Msa0755460:intron 15.0%
!!! AAATTGTAAATTCTTTTGTT+CGG + chr5_2:9249923-9249942 Msa0755460:intron 15.0%
!! AAAGTAACATAATTAGTATA+GGG + chr5_2:9250469-9250488 Msa0755460:CDS 15.0%
!!! CTTTTATTTACATATATGAT+TGG + chr5_2:9251263-9251282 Msa0755460:intron 15.0%
!! TTATTTCATAAAATTTGCAA+GGG - chr5_2:9251532-9251551 None:intergenic 15.0%
!! TTAATTTCATATTTGTAAGT+TGG - chr5_2:9252233-9252252 None:intergenic 15.0%
!! AAGATAAATTAATTGAACAT+TGG + chr5_2:9252613-9252632 Msa0755460:three_prime_UTR 15.0%
!!! TTATTTTATGATTATCTCAT+TGG - chr5_2:9252698-9252717 None:intergenic 15.0%
!! GTCAAAGAATATAATATTAT+AGG + chr5_2:9252734-9252753 Msa0755460:three_prime_UTR 15.0%
!!! GAACAATTATTATTCAATTT+TGG + chr5_2:9252794-9252813 Msa0755460:three_prime_UTR 15.0%
!!! ATTTTGTGTCTTTTTCTATT+TGG + chr5_2:9248850-9248869 Msa0755460:five_prime_UTR 20.0%
!! AAAGAACAAGTTCTTAAATA+TGG - chr5_2:9249001-9249020 None:intergenic 20.0%
!!! AAGCAACTGTTTTTTTTATA+AGG + chr5_2:9249037-9249056 Msa0755460:intron 20.0%
!! ATGATCATTCAAAATAATCA+TGG - chr5_2:9249149-9249168 None:intergenic 20.0%
!!! TTCTTTCTTTTTCATTGAAA+TGG - chr5_2:9249508-9249527 None:intergenic 20.0%
!!! TTCAGATGAATCTTTTAAAA+AGG + chr5_2:9249596-9249615 Msa0755460:intron 20.0%
!!! TCAGATGAATCTTTTAAAAA+GGG + chr5_2:9249597-9249616 Msa0755460:intron 20.0%
!!! CAAGTGATATTTACATTTTT+GGG + chr5_2:9249887-9249906 Msa0755460:CDS 20.0%
!!! CCATTTTATTATTCAAGTTT+AGG - chr5_2:9250031-9250050 None:intergenic 20.0%
!! CCTAAACTTGAATAATAAAA+TGG + chr5_2:9250028-9250047 Msa0755460:intron 20.0%
!! AAGTTATTTACAGAAAACTT+TGG - chr5_2:9250144-9250163 None:intergenic 20.0%
!! GAAAGTAACATAATTAGTAT+AGG + chr5_2:9250468-9250487 Msa0755460:CDS 20.0%
!! CTTATTTCATAAAATTTGCA+AGG - chr5_2:9251533-9251552 None:intergenic 20.0%
!!! TTCCCTTAATATTTTAAACT+TGG + chr5_2:9251762-9251781 Msa0755460:intron 20.0%
!!! GACCAAGTTTAAAATATTAA+GGG - chr5_2:9251767-9251786 None:intergenic 20.0%
!!! AGACCAAGTTTAAAATATTA+AGG - chr5_2:9251768-9251787 None:intergenic 20.0%
!! TCAAGTAAAATAAAAGGTTT+GGG - chr5_2:9251825-9251844 None:intergenic 20.0%
!! TTCAAGTAAAATAAAAGGTT+TGG - chr5_2:9251826-9251845 None:intergenic 20.0%
!!! AACCTTTTATTTTACTTGAA+TGG + chr5_2:9251826-9251845 Msa0755460:intron 20.0%
!!! ACCTTTTATTTTACTTGAAT+GGG + chr5_2:9251827-9251846 Msa0755460:intron 20.0%
!!! CTTTTATCTTATTATAGGTA+GGG - chr5_2:9251891-9251910 None:intergenic 20.0%
!!! ACAACCTTTTATCTTATTAT+AGG - chr5_2:9251896-9251915 None:intergenic 20.0%
!! TCAAATTTACATAGTAAGAA+AGG - chr5_2:9251945-9251964 None:intergenic 20.0%
!!! TTGATATTTTATTCCTCATA+AGG + chr5_2:9251961-9251980 Msa0755460:intron 20.0%
!! TAGATAACAAATTATAGATG+TGG - chr5_2:9252030-9252049 None:intergenic 20.0%
!! ATTAATTCAGATAGATATGT+TGG + chr5_2:9252248-9252267 Msa0755460:CDS 20.0%
!!! GACTATGAATATTAATTTGT+TGG + chr5_2:9252336-9252355 Msa0755460:intron 20.0%
!! ATAAAATTATATATGCATGC+AGG + chr5_2:9252375-9252394 Msa0755460:intron 20.0%
!! TTTTCTATTTGGTACTAAAG+AGG + chr5_2:9248861-9248880 Msa0755460:five_prime_UTR 25.0%
!!! TGCTGATTTGATTTTATTGT+AGG + chr5_2:9249173-9249192 Msa0755460:intron 25.0%
!! TGATTTTATTGTAGGCTTTT+TGG + chr5_2:9249181-9249200 Msa0755460:intron 25.0%
! ATATTCTCAACTCAAATATC+AGG + chr5_2:9249470-9249489 Msa0755460:CDS 25.0%
!!! TCAAATCACAGTTCTAAAAA+TGG - chr5_2:9249556-9249575 None:intergenic 25.0%
! TGCTCAGTATATAAATACTA+AGG + chr5_2:9249662-9249681 Msa0755460:CDS 25.0%
! ATATAAATACTAAGGAAGTG+AGG + chr5_2:9249670-9249689 Msa0755460:CDS 25.0%
! ATGCTATACTATACATAACT+AGG - chr5_2:9249708-9249727 None:intergenic 25.0%
!!! ATAACTTTGGTTTTTCTTCA+GGG + chr5_2:9249783-9249802 Msa0755460:intron 25.0%
! CCAAAAATGTAAATATCACT+TGG - chr5_2:9249889-9249908 None:intergenic 25.0%
!! CCAAGTGATATTTACATTTT+TGG + chr5_2:9249886-9249905 Msa0755460:CDS 25.0%
! ACTCATAGTTTACTGATAAA+TGG + chr5_2:9249950-9249969 Msa0755460:intron 25.0%
! CTCATAGTTTACTGATAAAT+GGG + chr5_2:9249951-9249970 Msa0755460:intron 25.0%
! CTGTAAATAACTTTCAATTG+TGG + chr5_2:9250151-9250170 Msa0755460:intron 25.0%
!!! TTTATGGTATTGATCTTGAT+TGG + chr5_2:9250514-9250533 Msa0755460:CDS 25.0%
! GAGATGTTTCACATTAAATT+AGG + chr5_2:9251003-9251022 Msa0755460:intron 25.0%
! TTCCACATTAATTGTTAACT+AGG + chr5_2:9251034-9251053 Msa0755460:intron 25.0%
! ATAAGTTAACACAAGTTGTT+AGG - chr5_2:9251563-9251582 None:intergenic 25.0%
! TGAACTAATGAATTATGATC+AGG + chr5_2:9251690-9251709 Msa0755460:CDS 25.0%
!! AAAGTTATGCATATTTTGCA+AGG - chr5_2:9251737-9251756 None:intergenic 25.0%
! ACCCATTCAAGTAAAATAAA+AGG - chr5_2:9251831-9251850 None:intergenic 25.0%
!! CCTTTTATCTTATTATAGGT+AGG - chr5_2:9251892-9251911 None:intergenic 25.0%
! CCTACCTATAATAAGATAAA+AGG + chr5_2:9251889-9251908 Msa0755460:intron 25.0%
! TGTTCACTATGAAAAAAAGA+AGG - chr5_2:9252779-9252798 None:intergenic 25.0%
! TGTCCAAAGATAAAATAACT+GGG - chr5_2:9252840-9252859 None:intergenic 25.0%
! TTGTCCAAAGATAAAATAAC+TGG - chr5_2:9252841-9252860 None:intergenic 25.0%
! TTTGAATGATGATGATGAGA+TGG - chr5_2:9248892-9248911 None:intergenic 30.0%
!! CTTTGACTTGAAACAATGAA+GGG + chr5_2:9249074-9249093 Msa0755460:exon 30.0%
!!! CATTTTTTCATCACAATGGA+AGG - chr5_2:9249207-9249226 None:intergenic 30.0%
CTTCCATTGTGATGAAAAAA+TGG + chr5_2:9249205-9249224 Msa0755460:CDS 30.0%
!!! AAGCCATTTTTTCATCACAA+TGG - chr5_2:9249211-9249230 None:intergenic 30.0%
ATGGCTTAATATTAAGCCTA+AGG + chr5_2:9249224-9249243 Msa0755460:CDS 30.0%
TCACTAAAATCATTCACCTT+AGG - chr5_2:9249243-9249262 None:intergenic 30.0%
! TCATCACTAATTTGCATTCT+TGG - chr5_2:9249419-9249438 None:intergenic 30.0%
! ATAGTCCATTTAGAACACAA+GGG + chr5_2:9249438-9249457 Msa0755460:CDS 30.0%
!!! CATAACTTTGGTTTTTCTTC+AGG + chr5_2:9249782-9249801 Msa0755460:intron 30.0%
! GTAAATATCACTTGGTTCTT+CGG - chr5_2:9249881-9249900 None:intergenic 30.0%
! TCAAGGGTAATTTTGTAAAC+TGG + chr5_2:9249983-9250002 Msa0755460:intron 30.0%
GAACAAATGATATTTGATCG+CGG - chr5_2:9250102-9250121 None:intergenic 30.0%
ATAATACACCTATCCAGAAA+TGG + chr5_2:9250241-9250260 Msa0755460:CDS 30.0%
TAATACACCTATCCAGAAAT+GGG + chr5_2:9250242-9250261 Msa0755460:CDS 30.0%
TCATCATTCAGCATATCAAT+TGG - chr5_2:9250405-9250424 None:intergenic 30.0%
TTAAGTAGTTCAGCTAGAAT+TGG + chr5_2:9250600-9250619 Msa0755460:CDS 30.0%
AACGAGAATGCTTTCATATA+TGG + chr5_2:9250633-9250652 Msa0755460:CDS 30.0%
TCTAACGAATCAACTACTTT+AGG - chr5_2:9250741-9250760 None:intergenic 30.0%
AATTTGAAAGTTTCACCTGT+TGG + chr5_2:9250945-9250964 Msa0755460:CDS 30.0%
AGCCTAGTTAACAATTAATG+TGG - chr5_2:9251039-9251058 None:intergenic 30.0%
CAAATCATCATGCTGAATAA+AGG + chr5_2:9251140-9251159 Msa0755460:intron 30.0%
! TTGTTAACAGTCAGATTTTC+TGG + chr5_2:9251593-9251612 Msa0755460:intron 30.0%
TCGAATCAGTATGTTAGATT+CGG + chr5_2:9251633-9251652 Msa0755460:CDS 30.0%
AGTAAAATAAAAGGTTTGGG+TGG - chr5_2:9251822-9251841 None:intergenic 30.0%
!!! TATAGGTAGGGTTTTTTATG+TGG - chr5_2:9251879-9251898 None:intergenic 30.0%
AATAGCAATAGTTCCTTATG+AGG - chr5_2:9251977-9251996 None:intergenic 30.0%
! TATTTGATGGATGGAAAGAA+GGG + chr5_2:9252192-9252211 Msa0755460:CDS 30.0%
ATTGCAATTAATTACCATGC+AGG - chr5_2:9252308-9252327 None:intergenic 30.0%
! ACCAGTTAGTTCAATTTTCT+TGG + chr5_2:9252478-9252497 Msa0755460:CDS 30.0%
ACCAAGAAAATTGAACTAAC+TGG - chr5_2:9252482-9252501 None:intergenic 30.0%
AAAAATTGACCATCTTCATC+AGG - chr5_2:9252587-9252606 None:intergenic 30.0%
!! TGTCCCAGTTATTTTATCTT+TGG + chr5_2:9252834-9252853 Msa0755460:three_prime_UTR 30.0%
! CCTTCATTGTTTCAAGTCAA+AGG - chr5_2:9249076-9249095 None:intergenic 35.0%
!! CCTTTGACTTGAAACAATGA+AGG + chr5_2:9249073-9249092 Msa0755460:exon 35.0%
AAGAAGAGGAAAAAGATCAG+AGG + chr5_2:9249097-9249116 Msa0755460:CDS 35.0%
ATTTGCATTCTTGGTTGCTT+AGG - chr5_2:9249410-9249429 None:intergenic 35.0%
! GATAGTCCATTTAGAACACA+AGG + chr5_2:9249437-9249456 Msa0755460:CDS 35.0%
TACAAAACAAGACACAGAAG+AGG + chr5_2:9249621-9249640 Msa0755460:CDS 35.0%
AATACTAAGGAAGTGAGGTA+TGG + chr5_2:9249675-9249694 Msa0755460:intron 35.0%
! TTTTTCTTCAGGGTAGCAAT+TGG + chr5_2:9249793-9249812 Msa0755460:intron 35.0%
TCAATATCAAGATCCTCAGA+TGG - chr5_2:9249844-9249863 None:intergenic 35.0%
! AGGATCTTGATATTGATGAC+TGG + chr5_2:9249848-9249867 Msa0755460:CDS 35.0%
TTACTGATAAATGGGCAGTA+CGG + chr5_2:9249959-9249978 Msa0755460:intron 35.0%
TAATGATTGCTTCCCATTTC+TGG - chr5_2:9250257-9250276 None:intergenic 35.0%
TTGCATGTGAGGAAAGTTTA+TGG + chr5_2:9250498-9250517 Msa0755460:CDS 35.0%
ATTTGCGACATTGCATCTAA+CGG - chr5_2:9250549-9250568 None:intergenic 35.0%
TTGATTCAAATCCGAAGTTG+CGG + chr5_2:9250571-9250590 Msa0755460:CDS 35.0%
GAGAATGCTTTCATATATGG+TGG + chr5_2:9250636-9250655 Msa0755460:CDS 35.0%
AATGCTTTCATATATGGTGG+TGG + chr5_2:9250639-9250658 Msa0755460:CDS 35.0%
ATTTGGGCTCGTTATTGAAA+GGG + chr5_2:9250703-9250722 Msa0755460:CDS 35.0%
ATTATCAGCAAGCAAATGGT+GGG + chr5_2:9250873-9250892 Msa0755460:CDS 35.0%
TGGGAATCTATGTATCTGTT+TGG + chr5_2:9250892-9250911 Msa0755460:CDS 35.0%
GGGAATCTATGTATCTGTTT+GGG + chr5_2:9250893-9250912 Msa0755460:CDS 35.0%
TCATCATGCTGAATAAAGGT+CGG + chr5_2:9251144-9251163 Msa0755460:intron 35.0%
GTTGAATTGAACTGATACAC+TGG - chr5_2:9251180-9251199 None:intergenic 35.0%
ACACATTTCTCAATGATGCA+GGG + chr5_2:9251295-9251314 Msa0755460:intron 35.0%
AGAACAAACAAAGCACATAC+GGG - chr5_2:9251353-9251372 None:intergenic 35.0%
GAGAACAAACAAAGCACATA+CGG - chr5_2:9251354-9251373 None:intergenic 35.0%
!!! AACAGTCAGATTTTCTGGTT+CGG + chr5_2:9251598-9251617 Msa0755460:intron 35.0%
AATCAGTATGTTAGATTCGG+AGG + chr5_2:9251636-9251655 Msa0755460:CDS 35.0%
! AATTGGTGGCTCAAAAAAAG+TGG + chr5_2:9251665-9251684 Msa0755460:CDS 35.0%
! TGCATATTTTGCAAGGTTTG+TGG - chr5_2:9251730-9251749 None:intergenic 35.0%
TAAGCAATGAATTGCGTGTT+GGG + chr5_2:9252165-9252184 Msa0755460:CDS 35.0%
! GTATTTGATGGATGGAAAGA+AGG + chr5_2:9252191-9252210 Msa0755460:CDS 35.0%
TTGGTGAGATTCCAAAAGAA+GGG + chr5_2:9252267-9252286 Msa0755460:CDS 35.0%
!!! CTTTTTTTCTCCCCTTCTTT+TGG - chr5_2:9252281-9252300 None:intergenic 35.0%
TCTTCATCAGGAAAAATCTC+AGG - chr5_2:9252575-9252594 None:intergenic 35.0%
! GAGATTTTTCCTGATGAAGA+TGG + chr5_2:9252575-9252594 Msa0755460:CDS 35.0%
TGAGTCAGTTACGTTACATA+AGG + chr5_2:9252667-9252686 Msa0755460:three_prime_UTR 35.0%
GAGTCAGTTACGTTACATAA+GGG + chr5_2:9252668-9252687 Msa0755460:three_prime_UTR 35.0%
AGTCAGTTACGTTACATAAG+GGG + chr5_2:9252669-9252688 Msa0755460:three_prime_UTR 35.0%
ATCTTTGGACAATAAACGTC+AGG + chr5_2:9252849-9252868 Msa0755460:three_prime_UTR 35.0%
TCTTTGGACAATAAACGTCA+GGG + chr5_2:9252850-9252869 Msa0755460:three_prime_UTR 35.0%
! TGTTTTCCTTGTGCAAGTTG+CGG + chr5_2:9248795-9248814 Msa0755460:five_prime_UTR 40.0%
GAAACAATGAAGGGAAGAAG+AGG + chr5_2:9249083-9249102 Msa0755460:exon 40.0%
GAAACTGAGAGTGAAGATGA+TGG + chr5_2:9249279-9249298 Msa0755460:CDS 40.0%
! TGAGTCCCTTGTGTTCTAAA+TGG - chr5_2:9249446-9249465 None:intergenic 40.0%
CTCAACTCAAATATCAGGCA+AGG + chr5_2:9249475-9249494 Msa0755460:intron 40.0%
ATTGGATCTTGGAATGTTGC+TGG + chr5_2:9249811-9249830 Msa0755460:CDS 40.0%
!! TACTGAAGCCGTTTTGTAGT+CGG + chr5_2:9250051-9250070 Msa0755460:intron 40.0%
AGTACATTTCCAGCATTCAG+TGG - chr5_2:9250210-9250229 None:intergenic 40.0%
CTGAATGCTGGAAATGTACT+AGG + chr5_2:9250210-9250229 Msa0755460:CDS 40.0%
! ATTGCTTCCCATTTCTGGAT+AGG - chr5_2:9250252-9250271 None:intergenic 40.0%
TATGCTGAATGATGAGCTCA+TGG + chr5_2:9250410-9250429 Msa0755460:CDS 40.0%
! TAGGGAATGATTTGCATGTG+AGG + chr5_2:9250487-9250506 Msa0755460:CDS 40.0%
CTTAATACTCTCCGCAACTT+CGG - chr5_2:9250585-9250604 None:intergenic 40.0%
AAACGTTCGCATCATAGTTC+CGG + chr5_2:9250678-9250697 Msa0755460:CDS 40.0%
CATCATAGTTCCGGAGATTT+GGG + chr5_2:9250687-9250706 Msa0755460:CDS 40.0%
GATTTGGGCTCGTTATTGAA+AGG + chr5_2:9250702-9250721 Msa0755460:CDS 40.0%
! ATCTGAGATGTTATCTGACG+AGG + chr5_2:9250767-9250786 Msa0755460:CDS 40.0%
! TCTGAGATGTTATCTGACGA+GGG + chr5_2:9250768-9250787 Msa0755460:CDS 40.0%
AATCACATGCGAGGTATGTT+CGG + chr5_2:9250850-9250869 Msa0755460:CDS 40.0%
TCGGATTATCAGCAAGCAAA+TGG + chr5_2:9250869-9250888 Msa0755460:CDS 40.0%
GATTATCAGCAAGCAAATGG+TGG + chr5_2:9250872-9250891 Msa0755460:CDS 40.0%
AAAGTTTCACCTGTTGGAGT+TGG + chr5_2:9250951-9250970 Msa0755460:CDS 40.0%
ATATCACTAGTGCACGAGTT+TGG - chr5_2:9251235-9251254 None:intergenic 40.0%
GACACATTTCTCAATGATGC+AGG + chr5_2:9251294-9251313 Msa0755460:intron 40.0%
GTTTGTTCTCATCTGACTTC+TGG + chr5_2:9251364-9251383 Msa0755460:CDS 40.0%
TCATCTGACTTCTGGTACAA+AGG + chr5_2:9251372-9251391 Msa0755460:CDS 40.0%
GTATGTTAGATTCGGAGGTT+CGG + chr5_2:9251641-9251660 Msa0755460:CDS 40.0%
CTAAGCAATGAATTGCGTGT+TGG + chr5_2:9252164-9252183 Msa0755460:CDS 40.0%
TTGGGCATGTATTTGATGGA+TGG + chr5_2:9252183-9252202 Msa0755460:CDS 40.0%
! GTTGGTGAGATTCCAAAAGA+AGG + chr5_2:9252266-9252285 Msa0755460:CDS 40.0%
TGGTGAGATTCCAAAAGAAG+GGG + chr5_2:9252268-9252287 Msa0755460:CDS 40.0%
AGAAAAAAAGAGCTCCTGCA+TGG + chr5_2:9252291-9252310 Msa0755460:CDS 40.0%
TGTGATCGAATATTGTGGCT+AGG + chr5_2:9252398-9252417 Msa0755460:CDS 40.0%
CGAATATTGTGGCTAGGAAA+AGG + chr5_2:9252404-9252423 Msa0755460:CDS 40.0%
GTCAGTTACGTTACATAAGG+GGG + chr5_2:9252670-9252689 Msa0755460:three_prime_UTR 40.0%
TCAGGGTAGCAATTGGATCT+TGG + chr5_2:9249800-9249819 Msa0755460:intron 45.0%
TAAATGGGCAGTACGGTTCA+AGG + chr5_2:9249966-9249985 Msa0755460:intron 45.0%
AAATGGGCAGTACGGTTCAA+GGG + chr5_2:9249967-9249986 Msa0755460:intron 45.0%
CTACGTCTCCGACTACAAAA+CGG - chr5_2:9250062-9250081 None:intergenic 45.0%
ACTACTTCCTGGAAACTGTG+AGG - chr5_2:9250186-9250205 None:intergenic 45.0%
CATTCAGTGGAACTACTTCC+TGG - chr5_2:9250197-9250216 None:intergenic 45.0%
GAAGTAGTTCCACTGAATGC+TGG + chr5_2:9250198-9250217 Msa0755460:CDS 45.0%
TGGAAATGTACTAGGAGCAG+AGG + chr5_2:9250218-9250237 Msa0755460:CDS 45.0%
!!! TGGCTTTTGTGTTTGCTGTC+AGG - chr5_2:9250306-9250325 None:intergenic 45.0%
GCAGAAGAAGTTCTCAACAC+CGG - chr5_2:9250345-9250364 None:intergenic 45.0%
! ATTGCATCTAACGGACGTTC+GGG - chr5_2:9250540-9250559 None:intergenic 45.0%
! CATTGCATCTAACGGACGTT+CGG - chr5_2:9250541-9250560 None:intergenic 45.0%
!! GCTTTCATATATGGTGGTGG+TGG + chr5_2:9250642-9250661 Msa0755460:CDS 45.0%
!! TTCATATATGGTGGTGGTGG+TGG + chr5_2:9250645-9250664 Msa0755460:CDS 45.0%
GCATCATAGTTCCGGAGATT+TGG + chr5_2:9250686-9250705 Msa0755460:CDS 45.0%
CAATAACGAGCCCAAATCTC+CGG - chr5_2:9250700-9250719 None:intergenic 45.0%
CGCTTAATCCATCAGCATCT+TGG - chr5_2:9250824-9250843 None:intergenic 45.0%
ATGTATCTGTTTGGGTGCAG+AGG + chr5_2:9250901-9250920 Msa0755460:CDS 45.0%
!! ACCTGTTGGAGTTGGTCTTA+TGG + chr5_2:9250959-9250978 Msa0755460:CDS 45.0%
!! CCTGTTGGAGTTGGTCTTAT+GGG + chr5_2:9250960-9250979 Msa0755460:CDS 45.0%
!! AGTTGGTCTTATGGGCTACA+TGG + chr5_2:9250968-9250987 Msa0755460:CDS 45.0%
!! GTTGGTCTTATGGGCTACAT+GGG + chr5_2:9250969-9250988 Msa0755460:CDS 45.0%
! TATGGGCTACATGGGAAACA+AGG + chr5_2:9250977-9250996 Msa0755460:CDS 45.0%
ACTACTTACCTTTGTCAGCG+AGG + chr5_2:9251198-9251217 Msa0755460:intron 45.0%
CTGACTTCTGGTACAAAGGA+TGG + chr5_2:9251376-9251395 Msa0755460:CDS 45.0%
ATAAAGCTCACTCGTGCGAT+GGG - chr5_2:9251492-9251511 None:intergenic 45.0%
AATAAAGCTCACTCGTGCGA+TGG - chr5_2:9251493-9251512 None:intergenic 45.0%
AGATTCGGAGGTTCGGAAAT+TGG + chr5_2:9251648-9251667 Msa0755460:CDS 45.0%
CGTGTTGGGCATGTATTTGA+TGG + chr5_2:9252179-9252198 Msa0755460:CDS 45.0%
GCAGGTGTGATCGAATATTG+TGG + chr5_2:9252393-9252412 Msa0755460:intron 45.0%
TTGGACAATAAACGTCAGGG+TGG + chr5_2:9252853-9252872 Msa0755460:three_prime_UTR 45.0%
TGGACAATAAACGTCAGGGT+GGG + chr5_2:9252854-9252873 Msa0755460:three_prime_UTR 45.0%
TCACAACCGCAACTTGCACA+AGG - chr5_2:9248804-9248823 None:intergenic 50.0%
TGCTGGAAGACATCCATCTG+AGG + chr5_2:9249828-9249847 Msa0755460:CDS 50.0%
! CGTTTTGTAGTCGGAGACGT+AGG + chr5_2:9250060-9250079 Msa0755460:intron 50.0%
TCTTGTGCCTCACAGTTTCC+AGG + chr5_2:9250176-9250195 Msa0755460:intron 50.0%
!! ATATATGGTGGTGGTGGTGG+AGG + chr5_2:9250648-9250667 Msa0755460:CDS 50.0%
! CCATCAGCATCTTGGTTCTC+AGG - chr5_2:9250816-9250835 None:intergenic 50.0%
CCTGAGAACCAAGATGCTGA+TGG + chr5_2:9250813-9250832 Msa0755460:CDS 50.0%
TATCTGTTTGGGTGCAGAGG+AGG + chr5_2:9250904-9250923 Msa0755460:CDS 50.0%
CCCATAAGACCAACTCCAAC+AGG - chr5_2:9250963-9250982 None:intergenic 50.0%
CTCTTTAGCCTCGCTGACAA+AGG - chr5_2:9251209-9251228 None:intergenic 50.0%
CTTTGTCAGCGAGGCTAAAG+AGG + chr5_2:9251207-9251226 Msa0755460:intron 50.0%
TTCGGAGGTTCGGAAATTGG+TGG + chr5_2:9251651-9251670 Msa0755460:CDS 50.0%
GAAGTTCTCAACACCGGCGA+AGG - chr5_2:9250339-9250358 None:intergenic 55.0%
GTTCTCAACACCGGCGAAGG+TGG - chr5_2:9250336-9250355 None:intergenic 60.0%
!! TATGGTGGTGGTGGTGGAGG+TGG + chr5_2:9250651-9250670 Msa0755460:CDS 60.0%
GCGCGACGAAATCACATGCG+AGG + chr5_2:9250841-9250860 Msa0755460:CDS 60.0%
CCACAGCGCGCCACCTTCGC+CGG + chr5_2:9250323-9250342 Msa0755460:CDS 75.0%
! CCGGCGAAGGTGGCGCGCTG+TGG - chr5_2:9250326-9250345 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 9248793 9252936 9248793 ID=Msa0755460;Name=Msa0755460
chr5_2 mRNA 9248793 9252936 9248793 ID=Msa0755460-mRNA-1;Parent=Msa0755460;Name=Msa0755460-mRNA-1;_AED=0.07;_eAED=0.07;_QI=164|1|1|1|0.7|0.72|11|326|629
chr5_2 exon 9248793 9248926 9248793 ID=Msa0755460-mRNA-1:exon:4294;Parent=Msa0755460-mRNA-1
chr5_2 exon 9249059 9249118 9249059 ID=Msa0755460-mRNA-1:exon:4295;Parent=Msa0755460-mRNA-1
chr5_2 exon 9249195 9249300 9249195 ID=Msa0755460-mRNA-1:exon:4296;Parent=Msa0755460-mRNA-1
chr5_2 exon 9249399 9249491 9249399 ID=Msa0755460-mRNA-1:exon:4297;Parent=Msa0755460-mRNA-1
chr5_2 exon 9249601 9249691 9249601 ID=Msa0755460-mRNA-1:exon:4298;Parent=Msa0755460-mRNA-1
chr5_2 exon 9249804 9249908 9249804 ID=Msa0755460-mRNA-1:exon:4299;Parent=Msa0755460-mRNA-1
chr5_2 exon 9250191 9250998 9250191 ID=Msa0755460-mRNA-1:exon:4300;Parent=Msa0755460-mRNA-1
chr5_2 exon 9251316 9251500 9251316 ID=Msa0755460-mRNA-1:exon:4301;Parent=Msa0755460-mRNA-1
chr5_2 exon 9251603 9251711 9251603 ID=Msa0755460-mRNA-1:exon:4302;Parent=Msa0755460-mRNA-1
chr5_2 exon 9252164 9252312 9252164 ID=Msa0755460-mRNA-1:exon:4303;Parent=Msa0755460-mRNA-1
chr5_2 exon 9252397 9252936 9252397 ID=Msa0755460-mRNA-1:exon:4304;Parent=Msa0755460-mRNA-1
chr5_2 five_prime_UTR 9248793 9248926 9248793 ID=Msa0755460-mRNA-1:five_prime_utr;Parent=Msa0755460-mRNA-1
chr5_2 five_prime_UTR 9249059 9249088 9249059 ID=Msa0755460-mRNA-1:five_prime_utr;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9249089 9249118 9249089 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9249195 9249300 9249195 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9249399 9249491 9249399 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9249601 9249691 9249601 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9249804 9249908 9249804 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9250191 9250998 9250191 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9251316 9251500 9251316 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9251603 9251711 9251603 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9252164 9252312 9252164 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 CDS 9252397 9252610 9252397 ID=Msa0755460-mRNA-1:cds;Parent=Msa0755460-mRNA-1
chr5_2 three_prime_UTR 9252611 9252936 9252611 ID=Msa0755460-mRNA-1:three_prime_utr;Parent=Msa0755460-mRNA-1
Gene Sequence

>Msa0755460

ATGAAGGGAAGAAGAGGAAAAAGATCAGAGGCTTTTTGGCCTTCCATTGTGATGAAAAAATGGCTTAATATTAAGCCTAAGGTGAATGATTTTAGTGAAGATGAAGTTGATACTGAAACTGAGAGTGAAGATGATGTTTGTTCTCCTAAGCAACCAAGAATGCAAATTAGTGATGATAGTCCATTTAGAACACAAGGGACTCAATCTATATTCTCAACTCAAATATCAGATGAATCTTTTAAAAAGGGATACAAAACAAGACACAGAAGAGGAAAATCAGAAACTCTGCGTGCTCAGTATATAAATACTAAGGAAGTGAGGGTAGCAATTGGATCTTGGAATGTTGCTGGAAGACATCCATCTGAGGATCTTGATATTGATGACTGGATTTGTGCCGAAGAACCAAGTGATATTTACATTTTTGGTTTCCAGGAAGTAGTTCCACTGAATGCTGGAAATGTACTAGGAGCAGAGGATAATACACCTATCCAGAAATGGGAAGCAATCATTAGAAGATCTTTGAACAAATCTTCTGAGCCTGACAGCAAACACAAAAGCCACAGCGCGCCACCTTCGCCGGTGTTGAGAACTTCTTCTGCTGCTGATGTTCTAGCTGATAATATAGATGCTGCTAATCCAATTGATATGCTGAATGATGAGCTCATGGAAAATGTTGACAAATATGATCTGCAACAGCTTCAAGAAAGTAACATAATTAGTATAGGGAATGATTTGCATGTGAGGAAAGTTTATGGTATTGATCTTGATTGGCCCGAACGTCCGTTAGATGCAATGTCGCAAATTGTTGATTCAAATCCGAAGTTGCGGAGAGTATTAAGTAGTTCAGCTAGAATTGGCTTTGACTTGAACGAGAATGCTTTCATATATGGTGGTGGTGGTGGAGGTGGATTGAAACGTTCGCATCATAGTTCCGGAGATTTGGGCTCGTTATTGAAAGGGCAAGAAGTTATACCTAAAGTAGTTGATTCGTTAGATGATGTATCTGAGATGTTATCTGACGAGGGTGATGATGCTTTTATTGAATTACCTGAGAACCAAGATGCTGATGGATTAAGCGCGACGAAATCACATGCGAGGTATGTTCGGATTATCAGCAAGCAAATGGTGGGAATCTATGTATCTGTTTGGGTGCAGAGGAGGCTGAGAAGACACATTAATAATTTGAAAGTTTCACCTGTTGGAGTTGGTCTTATGGGCTACATGGGAAACAAGGGTTCTGTTTCGATAAGCATGTCACTCTTTCAGTCCCGTATGTGCTTTGTTTGTTCTCATCTGACTTCTGGTACAAAGGATGGAGCTGAACAAAGACGAAACTCAGATGTTAATGAAATTCTACGACGCACTTGTTTCTCGTCTGTCTTTGCTACAGATCAAGCGCTAACAATCCCATCGCACGATCAGATTTTCTGGTTCGGAGATTTGAATTATCGAATCAGTATGTTAGATTCGGAGGTTCGGAAATTGGTGGCTCAAAAAAAGTGGAATGAACTAATGAATTATGATCAGCTAAGCAATGAATTGCGTGTTGGGCATGTATTTGATGGATGGAAAGAAGGGTTGATAAACTTTGCACCAACTTACAAATATGAAATTAATTCAGATAGATATGTTGGTGAGATTCCAAAAGAAGGGGAGAAAAAAAGAGCTCCTGCATGGTGTGATCGAATATTGTGGCTAGGAAAAGGAATAAAACAACTAAAATATGAACGTGCAGAAATTAAGCTCTCAGATCATAGACCAGTTAGTTCAATTTTCTTGGTTGAAGTTGAAGTATTTGATCATCGCAAATTAAGAAGAGCACTAAATTTCACTAATACTGCTGCAGTACATCCTGAGATTTTTCCTGATGAAGATGGTCAATTTTTCTAA
Protein sequence

>Msa0755460

MKGRRGKRSEAFWPSIVMKKWLNIKPKVNDFSEDEVDTETESEDDVCSPKQPRMQISDDSPFRTQGTQSIFSTQISDESFKKGYKTRHRRGKSETLRAQYINTKEVRVAIGSWNVAGRHPSEDLDIDDWICAEEPSDIYIFGFQEVVPLNAGNVLGAEDNTPIQKWEAIIRRSLNKSSEPDSKHKSHSAPPSPVLRTSSAADVLADNIDAANPIDMLNDELMENVDKYDLQQLQESNIISIGNDLHVRKVYGIDLDWPERPLDAMSQIVDSNPKLRRVLSSSARIGFDLNENAFIYGGGGGGGLKRSHHSSGDLGSLLKGQEVIPKVVDSLDDVSEMLSDEGDDAFIELPENQDADGLSATKSHARYVRIISKQMVGIYVSVWVQRRLRRHINNLKVSPVGVGLMGYMGNKGSVSISMSLFQSRMCFVCSHLTSGTKDGAEQRRNSDVNEILRRTCFSSVFATDQALTIPSHDQIFWFGDLNYRISMLDSEVRKLVAQKKWNELMNYDQLSNELRVGHVFDGWKEGLINFAPTYKYEINSDRYVGEIPKEGEKKRAPAWCDRILWLGKGIKQLKYERAEIKLSDHRPVSSIFLVEVEVFDHRKLRRALNFTNTAAVHPEIFPDEDGQFF*