AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0316470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316470 MtrunA17_Chr2g0288871 96.270 563 11 3 1 563 1 553 0.0 1109
Msa0316470 MtrunA17_Chr4g0075201 54.433 485 150 20 59 524 33 465 1.18e-138 411
Msa0316470 MtrunA17_Chr2g0322391 47.177 549 195 19 62 556 81 588 4.43e-126 382
Msa0316470 MtrunA17_Chr3g0099641 43.478 483 196 14 60 535 32 444 1.11e-108 333
Msa0316470 MtrunA17_Chr4g0025061 36.396 555 227 18 67 542 60 567 8.89e-87 281
Msa0316470 MtrunA17_Chr7g0270831 41.309 489 170 16 67 545 30 411 7.07e-86 273
Msa0316470 MtrunA17_Chr4g0024391 38.105 475 189 16 144 542 1 446 1.26e-74 245
Msa0316470 MtrunA17_Chr4g0025051 36.300 573 233 20 67 550 35 564 2.27e-74 247
Msa0316470 MtrunA17_Chr2g0302391 33.967 421 183 14 143 541 68 415 7.09e-56 194
Msa0316470 MtrunA17_Chr7g0216891 33.043 460 222 14 150 541 47 488 2.91e-55 196
Msa0316470 MtrunA17_Chr4g0060271 36.667 480 226 16 143 555 70 538 4.27e-46 171
Msa0316470 MtrunA17_Chr4g0051181 36.808 307 159 10 76 367 17 303 1.36e-45 167
Msa0316470 MtrunA17_Chr5g0419241 90.476 63 6 0 275 337 45 107 2.64e-35 128
Msa0316470 MtrunA17_Chr1g0150281 33.191 235 110 5 150 383 28 216 1.29e-28 115
Msa0316470 MtrunA17_Chr4g0001361 38.816 152 69 4 282 433 137 264 3.50e-27 112
Msa0316470 MtrunA17_Chr3g0101131 30.877 285 142 10 112 369 220 476 3.25e-25 110
Msa0316470 MtrunA17_Chr5g0445311 55.000 80 35 1 290 369 347 425 1.86e-24 107
Msa0316470 MtrunA17_Chr7g0218081 35.294 170 104 3 217 382 317 484 1.99e-24 107
Msa0316470 MtrunA17_Chr1g0150271 45.918 98 51 1 302 397 96 193 2.00e-24 102
Msa0316470 MtrunA17_Chr3g0136961 50.588 85 42 0 292 376 123 207 2.50e-24 103
Msa0316470 MtrunA17_Chr3g0079691 50.000 90 44 1 280 369 468 556 1.32e-23 105
Msa0316470 MtrunA17_Chr3g0079691 44.086 93 50 2 276 368 249 339 1.09e-16 83.6
Msa0316470 MtrunA17_Chr7g0270191 48.421 95 44 2 291 385 129 218 1.38e-23 101
Msa0316470 MtrunA17_Chr3g0106491 53.165 79 36 1 289 367 347 424 1.94e-23 103
Msa0316470 MtrunA17_Chr3g0106491 30.872 149 70 4 221 369 143 258 2.18e-11 66.2
Msa0316470 MtrunA17_Chr4g0004911 53.165 79 36 1 289 367 318 395 2.47e-23 103
Msa0316470 MtrunA17_Chr4g0004911 42.667 75 40 2 293 367 172 243 2.29e-14 75.9
Msa0316470 MtrunA17_Chr5g0432161 53.086 81 37 1 289 369 403 482 4.19e-23 103
Msa0316470 MtrunA17_Chr5g0432161 51.667 60 27 2 308 367 245 302 1.36e-13 73.6
Msa0316470 MtrunA17_Chr7g0243891 52.326 86 40 1 284 369 351 435 4.73e-23 103
Msa0316470 MtrunA17_Chr5g0418191 45.918 98 52 1 289 386 302 398 1.77e-22 100
Msa0316470 MtrunA17_Chr5g0418191 39.583 96 56 2 308 403 152 245 3.58e-13 71.6
Msa0316470 MtrunA17_Chr2g0333851 51.190 84 36 2 289 367 146 229 2.81e-22 97.8
Msa0316470 MtrunA17_Chr5g0447701 45.631 103 49 3 282 379 222 322 2.83e-22 99.0
Msa0316470 MtrunA17_Chr5g0447701 41.250 80 45 2 305 384 90 167 2.15e-11 65.9
Msa0316470 MtrunA17_Chr4g0042831 47.368 95 46 2 289 380 260 353 3.55e-22 99.0
Msa0316470 MtrunA17_Chr4g0051881 45.055 91 49 1 294 384 165 254 3.99e-22 97.8
Msa0316470 MtrunA17_Chr7g0249311 52.500 80 37 1 290 369 375 453 5.19e-22 100
Msa0316470 MtrunA17_Chr7g0249311 50.000 60 28 2 308 367 180 237 1.43e-11 67.4
Msa0316470 MtrunA17_Chr2g0294231 44.348 115 53 4 291 401 187 294 5.25e-22 98.2
Msa0316470 MtrunA17_Chr1g0150801 42.424 99 55 2 267 365 63 159 5.37e-22 92.8
Msa0316470 MtrunA17_Chr7g0249301 52.500 80 37 1 290 369 275 353 5.45e-22 98.2
Msa0316470 MtrunA17_Chr7g0249301 47.692 65 32 2 303 367 58 120 6.33e-12 67.4
Msa0316470 MtrunA17_Chr7g0224801 53.521 71 32 1 296 366 82 151 5.80e-22 93.2
Msa0316470 MtrunA17_Chr1g0194481 43.269 104 57 2 291 393 142 244 6.01e-21 94.0
Msa0316470 MtrunA17_Chr7g0263531 41.837 98 56 1 270 367 324 420 1.38e-20 94.7
Msa0316470 MtrunA17_Chr7g0263531 43.902 82 40 3 308 389 190 265 2.09e-12 69.3
Msa0316470 MtrunA17_Chr7g0270051 45.361 97 44 2 290 380 30 123 1.83e-20 91.3
Msa0316470 MtrunA17_Chr5g0418781 50.000 78 38 1 287 364 104 180 6.71e-20 90.1
Msa0316470 MtrunA17_Chr4g0068541 47.619 84 42 2 291 374 192 273 1.09e-19 91.7
Msa0316470 MtrunA17_Chr5g0443131 50.000 74 36 1 294 367 144 216 2.36e-19 89.0
Msa0316470 MtrunA17_Chr7g0248721 34.682 173 98 6 195 365 38 197 3.22e-19 86.7
Msa0316470 MtrunA17_Chr1g0147881 48.649 74 38 0 291 364 246 319 4.13e-19 89.0
Msa0316470 MtrunA17_Chr4g0074001 49.351 77 38 1 291 367 343 418 4.37e-19 90.5
Msa0316470 MtrunA17_Chr5g0401821 50.000 76 38 0 291 366 183 258 5.23e-19 87.4
Msa0316470 MtrunA17_Chr8g0335811 48.000 75 38 1 290 364 137 210 6.50e-19 85.9
Msa0316470 MtrunA17_Chr8g0379461 59.016 61 25 0 307 367 76 136 6.81e-19 86.3
Msa0316470 MtrunA17_Chr4g0053281 49.367 79 40 0 291 369 250 328 8.22e-19 88.2
Msa0316470 MtrunA17_Chr2g0293481 51.316 76 37 0 307 382 267 342 1.38e-18 89.0
Msa0316470 MtrunA17_Chr1g0184691 46.429 84 45 0 291 374 277 360 1.66e-18 87.4
Msa0316470 MtrunA17_Chr2g0284321 56.452 62 27 0 307 368 35 96 1.98e-18 84.3
Msa0316470 MtrunA17_Chr2g0319961 54.286 70 32 0 298 367 71 140 2.63e-18 85.1
Msa0316470 MtrunA17_Chr3g0129361 46.667 75 39 1 290 364 127 200 3.05e-18 84.0
Msa0316470 MtrunA17_Chr8g0386241 45.570 79 42 1 292 370 88 165 4.01e-18 82.4
Msa0316470 MtrunA17_Chr3g0129321 47.297 74 38 1 294 367 105 177 5.13e-18 82.8
Msa0316470 MtrunA17_Chr7g0228941 49.296 71 36 0 294 364 39 109 7.50e-18 80.1
Msa0316470 MtrunA17_Chr1g0188941 41.667 84 48 1 284 367 28 110 7.98e-18 79.3
Msa0316470 MtrunA17_Chr7g0248691 50.000 74 37 0 304 377 151 224 1.58e-17 84.0
Msa0316470 MtrunA17_Chr4g0071521 50.000 74 37 0 291 364 229 302 1.61e-17 84.0
Msa0316470 MtrunA17_Chr2g0289181 34.074 135 79 3 302 426 9 143 4.25e-17 80.9
Msa0316470 MtrunA17_Chr8g0383351 46.053 76 39 2 290 364 142 216 7.10e-17 80.1
Msa0316470 MtrunA17_Chr8g0348311 49.296 71 36 0 307 377 155 225 7.97e-17 82.0
Msa0316470 MtrunA17_Chr3g0139021 47.297 74 39 0 291 364 298 371 8.21e-17 82.8
Msa0316470 MtrunA17_Chr8g0383251 45.946 74 39 1 294 367 95 167 1.84e-16 77.8
Msa0316470 MtrunA17_Chr8g0387501 44.444 72 40 0 306 377 128 199 1.13e-15 77.4
Msa0316470 MtrunA17_Chr6g0485431 46.667 75 38 2 294 367 141 214 1.08e-14 75.9
Msa0316470 MtrunA17_Chr7g0238921 37.190 121 59 3 267 372 70 188 1.45e-14 75.1
Msa0316470 MtrunA17_Chr1g0202461 46.479 71 38 0 307 377 261 331 2.29e-14 75.1
Msa0316470 MtrunA17_Chr1g0189391 43.056 72 39 2 294 364 84 154 1.33e-13 72.4
Msa0316470 MtrunA17_Chr5g0432071 41.558 77 43 2 308 384 105 179 1.72e-13 69.7
Msa0316470 MtrunA17_Chr3g0126391 54.237 59 25 1 306 362 132 190 3.98e-13 70.9
Msa0316470 MtrunA17_Chr8g0347251 49.275 69 28 2 306 367 135 203 4.53e-12 67.8
Msa0316470 MtrunA17_Chr7g0244971 35.294 102 63 2 288 386 103 204 7.29e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316470 AT1G62300.1 50.990 606 181 30 1 544 1 552 4.41e-153 450
Msa0316470 AT4G04450.1 52.299 522 158 23 62 535 25 503 1.95e-140 417
Msa0316470 AT4G04450.2 55.088 452 119 21 125 535 1 409 1.50e-128 383
Msa0316470 AT4G22070.4 52.990 485 167 19 43 479 1 472 4.92e-120 365
Msa0316470 AT4G22070.3 52.990 485 167 19 43 479 1 472 4.92e-120 365
Msa0316470 AT4G22070.2 52.990 485 167 19 43 479 1 472 4.92e-120 365
Msa0316470 AT4G22070.1 52.990 485 167 19 43 479 1 472 4.92e-120 365
Msa0316470 AT4G01720.1 42.032 433 179 15 110 532 73 443 5.41e-64 218
Msa0316470 AT5G15130.1 34.389 442 230 12 143 536 35 464 5.55e-50 181
Msa0316470 AT1G68150.1 40.711 253 108 8 146 388 96 316 6.93e-49 174
Msa0316470 AT5G15130.2 34.332 434 225 12 151 536 1 422 1.93e-48 176
Msa0316470 AT1G18860.1 32.365 482 250 12 132 537 30 511 1.28e-43 163
Msa0316470 AT1G69810.1 33.846 325 191 8 128 437 14 329 1.66e-41 154
Msa0316470 AT1G69810.2 53.691 149 63 3 290 437 128 271 5.54e-37 140
Msa0316470 AT4G31800.1 34.156 243 123 5 145 385 47 254 1.28e-31 125
Msa0316470 AT1G80840.1 31.641 256 114 7 116 367 8 206 4.48e-29 117
Msa0316470 AT4G31800.2 50.505 99 44 2 292 385 91 189 6.70e-25 104
Msa0316470 AT2G38470.1 49.451 91 45 1 289 379 344 433 7.94e-25 108
Msa0316470 AT2G38470.1 51.667 60 27 2 308 367 185 242 2.43e-13 73.2
Msa0316470 AT4G26640.5 53.846 78 35 1 290 367 188 264 6.49e-24 104
Msa0316470 AT4G26640.5 37.363 91 55 2 277 367 5 93 5.27e-13 71.2
Msa0316470 AT4G26640.6 53.846 78 35 1 290 367 188 264 6.49e-24 104
Msa0316470 AT4G26640.6 37.363 91 55 2 277 367 5 93 5.27e-13 71.2
Msa0316470 AT4G26640.4 53.846 78 35 1 290 367 188 264 6.49e-24 104
Msa0316470 AT4G26640.4 37.363 91 55 2 277 367 5 93 5.27e-13 71.2
Msa0316470 AT4G26640.3 53.846 78 35 1 290 367 188 264 6.49e-24 104
Msa0316470 AT4G26640.3 37.363 91 55 2 277 367 5 93 5.27e-13 71.2
Msa0316470 AT2G25000.2 48.864 88 45 0 285 372 81 168 1.07e-23 100
Msa0316470 AT2G25000.4 48.864 88 45 0 285 372 81 168 1.07e-23 100
Msa0316470 AT2G25000.3 46.237 93 50 0 280 372 98 190 1.31e-23 100
Msa0316470 AT4G26640.1 53.846 78 35 1 290 367 292 368 1.54e-23 104
Msa0316470 AT4G26640.1 37.363 91 55 2 277 367 109 197 6.25e-13 71.6
Msa0316470 AT4G26640.7 53.846 78 35 1 290 367 324 400 1.64e-23 104
Msa0316470 AT4G26640.7 37.363 91 55 2 277 367 141 229 6.48e-13 71.6
Msa0316470 AT1G29860.1 36.774 155 86 4 248 396 82 230 1.85e-23 100
Msa0316470 AT2G25000.1 48.864 88 45 0 285 372 124 211 2.17e-23 100
Msa0316470 AT1G13960.2 53.571 84 38 1 284 367 233 315 2.23e-23 102
Msa0316470 AT1G13960.2 51.667 60 27 2 308 367 77 134 2.02e-12 69.3
Msa0316470 AT4G26640.2 53.846 78 35 1 290 367 364 440 2.23e-23 104
Msa0316470 AT4G26640.2 37.363 91 55 2 277 367 181 269 7.47e-13 71.6
Msa0316470 AT1G13960.1 53.571 84 38 1 284 367 386 468 2.72e-23 103
Msa0316470 AT1G13960.1 51.667 60 27 2 308 367 230 287 1.38e-12 70.5
Msa0316470 AT5G26170.1 51.389 72 34 1 293 364 99 169 4.11e-23 97.1
Msa0316470 AT5G56270.1 49.438 89 44 1 281 369 461 548 5.52e-23 103
Msa0316470 AT5G56270.1 50.820 61 28 2 308 368 274 332 5.84e-12 68.9
Msa0316470 AT5G49520.1 51.948 77 36 1 291 367 205 280 9.00e-23 101
Msa0316470 AT4G18170.1 48.913 92 45 2 277 367 141 231 9.52e-23 99.8
Msa0316470 AT2G03340.1 51.163 86 41 1 284 369 392 476 1.93e-22 101
Msa0316470 AT2G03340.1 51.667 60 27 2 308 367 251 308 5.43e-13 72.0
Msa0316470 AT5G07100.2 47.126 87 45 1 281 367 115 200 2.82e-22 95.9
Msa0316470 AT5G07100.2 41.096 73 42 1 308 380 25 96 1.96e-11 64.3
Msa0316470 AT5G07100.4 47.126 87 45 1 281 367 115 200 2.82e-22 95.9
Msa0316470 AT5G07100.4 41.096 73 42 1 308 380 25 96 1.96e-11 64.3
Msa0316470 AT5G07100.5 47.126 87 45 1 281 367 115 200 2.82e-22 95.9
Msa0316470 AT5G07100.5 41.096 73 42 1 308 380 25 96 1.96e-11 64.3
Msa0316470 AT5G07100.3 47.126 87 45 1 281 367 115 200 2.82e-22 95.9
Msa0316470 AT5G07100.3 41.096 73 42 1 308 380 25 96 1.96e-11 64.3
Msa0316470 AT2G04880.2 45.349 86 46 1 287 372 263 347 3.44e-22 100
Msa0316470 AT2G04880.2 46.970 66 33 2 305 370 109 172 3.07e-12 69.3
Msa0316470 AT2G04880.1 44.828 87 47 1 286 372 286 371 4.32e-22 100
Msa0316470 AT2G04880.1 46.970 66 33 2 305 370 109 172 3.68e-12 69.3
Msa0316470 AT5G07100.1 47.126 87 45 1 281 367 208 293 4.92e-22 97.4
Msa0316470 AT5G07100.1 41.096 73 42 1 308 380 118 189 3.54e-11 65.1
Msa0316470 AT1G64000.1 48.235 85 43 1 281 365 88 171 6.18e-22 94.4
Msa0316470 AT5G46350.1 48.864 88 43 2 294 380 170 256 1.04e-21 96.7
Msa0316470 AT3G01080.1 47.619 84 43 1 284 367 283 365 3.34e-21 96.7
Msa0316470 AT3G01080.1 51.667 60 27 2 308 367 168 225 2.13e-11 66.6
Msa0316470 AT3G01080.2 47.619 84 43 1 284 367 321 403 3.78e-21 97.1
Msa0316470 AT3G01080.2 51.667 60 27 2 308 367 206 263 2.05e-11 66.6
Msa0316470 AT2G30250.1 51.250 80 38 1 290 369 311 389 6.17e-21 95.5
Msa0316470 AT2G47260.1 43.243 111 58 2 283 389 150 259 6.75e-21 94.7
Msa0316470 AT2G46130.1 48.780 82 41 1 284 365 7 87 1.07e-20 87.8
Msa0316470 AT1G55600.1 44.444 90 49 1 280 369 280 368 1.50e-20 95.5
Msa0316470 AT5G13080.1 43.000 100 56 1 267 366 27 125 1.58e-20 88.6
Msa0316470 AT4G30935.1 44.554 101 45 3 291 383 315 412 3.76e-20 94.0
Msa0316470 AT4G26440.1 45.545 101 52 2 291 391 356 453 5.04e-20 94.4
Msa0316470 AT2G30590.1 50.000 82 40 1 291 371 297 378 1.04e-19 91.7
Msa0316470 AT3G01970.1 43.137 102 57 1 263 364 21 121 1.04e-19 86.3
Msa0316470 AT1G29280.1 51.429 70 34 0 298 367 65 134 1.25e-19 89.4
Msa0316470 AT4G31800.3 31.683 202 101 5 145 344 47 213 1.76e-19 88.2
Msa0316470 AT1G69310.4 42.353 85 48 1 280 364 120 203 1.86e-19 89.4
Msa0316470 AT1G69310.2 42.353 85 48 1 280 364 120 203 1.86e-19 89.4
Msa0316470 AT1G69310.1 42.353 85 48 1 280 364 120 203 1.86e-19 89.4
Msa0316470 AT5G41570.1 55.556 72 31 1 294 365 85 155 2.13e-19 86.7
Msa0316470 AT2G44745.1 40.909 88 51 1 277 364 115 201 2.60e-19 87.4
Msa0316470 AT2G23320.1 57.627 59 25 0 308 366 241 299 4.58e-19 89.0
Msa0316470 AT5G64810.1 47.887 71 36 1 294 364 97 166 5.04e-19 85.9
Msa0316470 AT3G62340.1 50.000 78 38 1 294 371 105 181 1.18e-18 86.7
Msa0316470 AT4G24240.1 50.000 76 38 0 291 366 265 340 1.18e-18 88.2
Msa0316470 AT5G28650.1 48.649 74 38 0 291 364 246 319 1.99e-18 87.0
Msa0316470 AT3G58710.2 52.857 70 33 0 298 367 61 130 2.35e-18 85.9
Msa0316470 AT3G58710.1 52.857 70 33 0 298 367 62 131 2.39e-18 85.9
Msa0316470 AT4G39410.1 43.902 82 45 1 283 364 199 279 2.72e-18 86.3
Msa0316470 AT2G34830.1 48.193 83 42 1 293 374 200 282 8.81e-18 86.3
Msa0316470 AT1G30650.1 48.193 83 42 1 293 374 202 284 1.12e-17 86.3
Msa0316470 AT2G34830.2 48.193 83 42 1 293 374 209 291 1.12e-17 86.3
Msa0316470 AT3G04670.1 47.297 74 39 0 291 364 246 319 1.15e-17 84.7
Msa0316470 AT2G24570.1 48.649 74 38 0 291 364 227 300 1.23e-17 84.7
Msa0316470 AT4G23550.1 45.679 81 44 0 302 382 135 215 1.96e-17 84.0
Msa0316470 AT4G31550.1 47.297 74 39 0 291 364 230 303 2.46e-17 84.0
Msa0316470 AT4G31550.2 47.297 74 39 0 291 364 229 302 2.57e-17 83.6
Msa0316470 AT4G01250.1 49.296 71 36 0 307 377 128 198 3.35e-17 82.8
Msa0316470 AT2G37260.2 49.333 75 34 2 302 373 262 335 7.23e-17 82.8
Msa0316470 AT2G37260.2 42.105 76 42 2 308 383 84 157 9.02e-14 73.2
Msa0316470 AT2G37260.3 49.333 75 34 2 302 373 264 337 7.24e-17 82.8
Msa0316470 AT2G37260.3 42.105 76 42 2 308 383 86 159 8.84e-14 73.6
Msa0316470 AT5G52830.1 46.753 77 41 0 301 377 159 235 1.07e-16 82.0
Msa0316470 AT2G37260.1 49.333 75 34 2 302 373 344 417 1.46e-16 82.8
Msa0316470 AT2G37260.1 42.105 76 42 2 308 383 166 239 1.46e-13 73.2
Msa0316470 AT2G46130.2 45.122 82 40 2 284 365 7 83 8.75e-16 73.9
Msa0316470 AT1G69310.3 38.824 85 47 2 280 364 120 199 1.69e-14 74.7
Msa0316470 AT5G45050.1 54.098 61 28 0 307 367 1180 1240 2.94e-14 76.6
Msa0316470 AT5G45050.4 50.000 76 36 1 292 367 1028 1101 3.13e-14 76.6
Msa0316470 AT5G45050.2 54.098 61 28 0 307 367 1152 1212 3.21e-14 76.6
Msa0316470 AT2G21900.1 43.939 66 36 1 302 367 104 168 6.14e-14 71.2
Msa0316470 AT5G45260.1 39.216 102 55 3 283 382 1189 1285 2.92e-13 73.6
Msa0316470 AT5G43290.1 41.667 72 40 2 294 364 100 170 3.72e-13 70.5
Msa0316470 AT2G46400.1 41.772 79 44 1 286 362 83 161 9.13e-12 66.6
Msa0316470 AT3G01080.3 51.667 60 27 2 308 367 206 263 9.25e-12 67.0
Msa0316470 AT2G40740.1 47.297 74 34 2 308 376 174 247 2.74e-11 65.1
Msa0316470 AT2G40740.3 47.297 74 34 2 308 376 183 256 4.15e-11 64.7

Find 118 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCTTGTGTTTCTGATTC+TGG 0.066609 2_4:+13605617 None:intergenic
CTAAATATTCCTTTGTTCTT+AGG 0.146906 2_4:-13604383 Msa0316470:exon
ATAGCTCTTGCTAATAAATT+TGG 0.198143 2_4:+13604880 None:intergenic
CTTTGAAATTGCAATGGATT+TGG 0.199677 2_4:+13604772 None:intergenic
TTTGTTTATTATATATTATA+TGG 0.269132 2_4:-13607025 Msa0316470:exon
TTGACAGGAAATCCTAAAAT+AGG 0.296916 2_4:+13606883 None:intergenic
GCGAGAACAGCGGTGAAATT+AGG 0.306985 2_4:+13604511 None:intergenic
TTTGAAATTGCAATGGATTT+GGG 0.309204 2_4:+13604773 None:intergenic
GCTGCTATCTCCTCCATTAT+TGG 0.315481 2_4:-13604487 Msa0316470:CDS
TGAGAGTGAGATATTGGAAT+TGG 0.324537 2_4:+13606961 None:intergenic
AAAGGGTGAGAGTGAGATAT+TGG 0.336997 2_4:+13606955 None:intergenic
GGATGCCAATGGAGGAAATA+TGG 0.352069 2_4:-13605363 Msa0316470:CDS
CGCCGTTGGTGTTACGGGTA+AGG 0.353888 2_4:+13606860 None:intergenic
TAAATCCAATATCATCAAAA+AGG 0.354383 2_4:-13606746 Msa0316470:CDS
GTCTTCGCCGTTGGTGTTAC+GGG 0.361102 2_4:+13606855 None:intergenic
TGCATGGCCACCACCAATAA+TGG 0.361861 2_4:+13604474 None:intergenic
CTTGAGATAGGTACTACTAA+TGG 0.365396 2_4:-13605749 Msa0316470:CDS
CGTCTTCGCCGTTGGTGTTA+CGG 0.365794 2_4:+13606854 None:intergenic
CAAGTACCTAGTTTCTTTCA+AGG 0.368680 2_4:-13604727 Msa0316470:CDS
AGGAATAGCAATGGTAAAAC+TGG 0.382516 2_4:-13605653 Msa0316470:CDS
CTGCAAAGCCGTGTAATTAC+CGG 0.385725 2_4:+13606247 None:intergenic
AAAGAGATTAGATATGTTGA+AGG 0.386115 2_4:+13607092 None:intergenic
AAACTCACTTGTTTGCGAAC+TGG 0.388176 2_4:+13605273 None:intergenic
TTTGTCCATATTTCCTCCAT+TGG 0.390389 2_4:+13605358 None:intergenic
TACTACAGATTGATCACTTC+CGG 0.396054 2_4:+13606534 None:intergenic
AAACTAGGTACTTGATGAAA+TGG 0.398773 2_4:+13604736 None:intergenic
CTTCAATTGTCTCAAGAAGT+TGG 0.400880 2_4:-13604643 Msa0316470:CDS
CCAAGACAATTTCTTGAGAT+AGG 0.405230 2_4:-13605761 Msa0316470:CDS
CCCCTCAATCCCAAAACATA+TGG 0.412646 2_4:+13604255 None:intergenic
GCAGCAAGCATGTTACTATC+AGG 0.425873 2_4:-13604934 Msa0316470:CDS
GGCGGTGATAGCAGCGGTGG+CGG 0.446253 2_4:+13604537 None:intergenic
GCCATAGCTGCTGGTGGTAA+TGG 0.452891 2_4:+13604979 None:intergenic
ATCTCCTCCATTATTGGTGG+TGG 0.457394 2_4:-13604481 Msa0316470:CDS
CTATAGTATGCTCGAGGACA+TGG 0.465899 2_4:+13605318 None:intergenic
GGCGGTGACCGGAAAATAAC+CGG 0.466327 2_4:-13606832 Msa0316470:CDS
GAGATGCTAAGTCATGTTAC+CGG 0.472299 2_4:-13606266 Msa0316470:CDS
GAGAGAGTAAAGAGAGGGAG+AGG 0.480193 2_4:+13607120 None:intergenic
AGCATACTATAGATGCACTA+TGG 0.487105 2_4:-13605307 Msa0316470:CDS
ACGGCGAAGACGGCGGTGAC+CGG 0.490027 2_4:-13606843 Msa0316470:CDS
TATATTATATGGACAAAGCA+TGG 0.490606 2_4:-13607014 Msa0316470:exon
GTGTTACGGGTAAGGTTGAC+AGG 0.491562 2_4:+13606868 None:intergenic
ATTTGCTTGATCTGCAACAT+TGG 0.493278 2_4:+13605559 None:intergenic
TGCTAGATCAGAAGCTTCAA+TGG 0.495447 2_4:-13605498 Msa0316470:intron
TGTTGTTGCTATTGTTTGCA+TGG 0.495497 2_4:+13604458 None:intergenic
TGCCGAGGACAAAACAATCT+TGG 0.496599 2_4:-13605031 Msa0316470:CDS
TGTTGGTTGTGTTCATTTGA+AGG 0.504024 2_4:+13606772 None:intergenic
CAGAATCAGAAACACAAGGC+TGG 0.504762 2_4:-13605616 Msa0316470:CDS
ATTACGTACCTCAGTAGTCT+TGG 0.513802 2_4:+13606474 None:intergenic
AATAGGAAACATTCTATCGT+TGG 0.514918 2_4:+13606900 None:intergenic
AGAATCAGAAACACAAGGCT+GGG 0.517077 2_4:-13605615 Msa0316470:CDS
CCATTGCTATTCCTCATCTG+AGG 0.520104 2_4:+13605662 None:intergenic
GTGAGATCTAATGTAACAGT+AGG 0.521520 2_4:+13604808 None:intergenic
ATATTATATGGACAAAGCAT+GGG 0.526634 2_4:-13607013 Msa0316470:exon
GATCAATCTGTAGTAGATGA+TGG 0.527093 2_4:-13606526 Msa0316470:CDS
AATTGCAAGTGGAGCTTCAA+CGG 0.528483 2_4:-13606316 Msa0316470:CDS
ATATATAATAAACAAACAAA+AGG 0.528509 2_4:+13607032 None:intergenic
GTCACCGCCGTCTTCGCCGT+TGG 0.533790 2_4:+13606846 None:intergenic
GGATCAATGTCAAGTGCAGA+TGG 0.534800 2_4:-13604913 Msa0316470:CDS
ATAGCAATGGTAAAACTGGT+AGG 0.540414 2_4:-13605649 Msa0316470:CDS
AGAGAAGAAATCGACTTCAC+CGG 0.540783 2_4:+13606813 None:intergenic
ATCTTGGTAACAACATATGA+AGG 0.541003 2_4:-13605015 Msa0316470:CDS
TCCATTACCACCAGCAGCTA+TGG 0.541740 2_4:-13604980 Msa0316470:CDS
GCTATCTCCTCCATTATTGG+TGG 0.542504 2_4:-13604484 Msa0316470:CDS
CGTAACACCAACGGCGAAGA+CGG 0.544973 2_4:-13606853 Msa0316470:CDS
GATGCCATTGCCATAGCTGC+TGG 0.545474 2_4:+13604970 None:intergenic
CCAAGGTGTTGGTGGAGCAA+TGG 0.549924 2_4:-13605786 Msa0316470:CDS
GAATCACCACACTGAAAATG+AGG 0.553361 2_4:-13606198 Msa0316470:intron
TGATATTGGATTTAACATGT+TGG 0.558451 2_4:+13606755 None:intergenic
CCATTGCTCCACCAACACCT+TGG 0.560146 2_4:+13605786 None:intergenic
TCTACCTTGTTCAACAAGCA+TGG 0.561460 2_4:-13604857 Msa0316470:CDS
ATTAACATGGATATTAGAAG+TGG 0.561723 2_4:+13606696 None:intergenic
GAATACGAGAGAGTAAAGAG+AGG 0.565830 2_4:+13607114 None:intergenic
CCTATCTCAAGAAATTGTCT+TGG 0.566117 2_4:+13605761 None:intergenic
TCAGCTTGCACAATTGCAAG+TGG 0.569056 2_4:-13606327 Msa0316470:intron
AATACGAGAGAGTAAAGAGA+GGG 0.570641 2_4:+13607115 None:intergenic
ATATAATTGCAGATAAGTGA+TGG 0.573518 2_4:-13605384 Msa0316470:intron
TCATGTTACCGGTAATTACA+CGG 0.577854 2_4:-13606255 Msa0316470:CDS
TTGTTGGCTTGGAGCTTGTG+TGG 0.580944 2_4:+13604606 None:intergenic
CTGACCGAGTCAGCTAAAGA+AGG 0.586174 2_4:+13604562 None:intergenic
TTCTGTCCTTGAAAGAAACT+AGG 0.586929 2_4:+13604721 None:intergenic
TGAGGTCTTTGAAATTGCAA+TGG 0.588002 2_4:+13604766 None:intergenic
ACATGGATATTAGAAGTGGA+AGG 0.594815 2_4:+13606700 None:intergenic
TAACATGCTTGCTGCTGCTG+AGG 0.595156 2_4:+13604942 None:intergenic
TAGCAATGGTAAAACTGGTA+GGG 0.595770 2_4:-13605648 Msa0316470:CDS
ACAGCCTTCTTTAGCTGACT+CGG 0.597997 2_4:-13604566 Msa0316470:CDS
TAAGGAAGCTTACATTAACA+TGG 0.598862 2_4:+13606683 None:intergenic
TCGTTGGTATAAGTAGATGA+TGG 0.603216 2_4:+13606916 None:intergenic
AACCTTACCCGTAACACCAA+CGG 0.603945 2_4:-13606862 Msa0316470:CDS
AATGAGAAAAGCTCGTGTCT+CGG 0.604308 2_4:-13605525 Msa0316470:CDS
TCTAATGTAACAGTAGGGAA+TGG 0.608110 2_4:+13604814 None:intergenic
GGCTTTGCAGATGCAACTTG+TGG 0.608912 2_4:-13606234 Msa0316470:CDS
GGTTTACAACTTGCAAACAC+CGG 0.613072 2_4:-13606553 Msa0316470:CDS
ATCGGCGGTGATAGCAGCGG+TGG 0.617279 2_4:+13604534 None:intergenic
TGAGATCTAATGTAACAGTA+GGG 0.618128 2_4:+13604809 None:intergenic
GAGTCAGCTAAAGAAGGCTG+TGG 0.624335 2_4:+13604568 None:intergenic
GATCAAACACACCTCAGATG+AGG 0.626860 2_4:-13605673 Msa0316470:CDS
ACCACCAGCAGCTATGGCAA+TGG 0.626876 2_4:-13604974 Msa0316470:CDS
ATAGGTTCAACGTTGTGCCG+AGG 0.627663 2_4:-13605046 Msa0316470:intron
TATAGATGCACTATGGCAGT+CGG 0.632166 2_4:-13605300 Msa0316470:CDS
TGATGAAATGGTGCATGTTG+AGG 0.633017 2_4:+13604748 None:intergenic
TAGCTGCTGGTGGTAATGGA+TGG 0.633923 2_4:+13604983 None:intergenic
AACAAGGAACCTAAGAACAA+AGG 0.634270 2_4:+13604374 None:intergenic
GTTGCCATGCTTGTTGAACA+AGG 0.639105 2_4:+13604853 None:intergenic
GAATCAGAAACACAAGGCTG+GGG 0.640268 2_4:-13605614 Msa0316470:CDS
GATAGCAGCTGCGAGAACAG+CGG 0.642227 2_4:+13604501 None:intergenic
AGCGGTGAAATTAGGATCGG+CGG 0.644865 2_4:+13604519 None:intergenic
AACAGCGGTGAAATTAGGAT+CGG 0.645945 2_4:+13604516 None:intergenic
TAAGTGATGGATGCCAATGG+AGG 0.670782 2_4:-13605371 Msa0316470:CDS
AACACCAACGGCGAAGACGG+CGG 0.671264 2_4:-13606850 Msa0316470:CDS
GTGCATCTATAGTATGCTCG+AGG 0.675667 2_4:+13605312 None:intergenic
CCTCAGATGAGGAATAGCAA+TGG 0.676716 2_4:-13605662 Msa0316470:CDS
TATTATATGGACAAAGCATG+GGG 0.678619 2_4:-13607012 Msa0316470:exon
AGGATCGGCGGTGATAGCAG+CGG 0.681008 2_4:+13604531 None:intergenic
GCCATTGCCATAGCTGCTGG+TGG 0.689582 2_4:+13604973 None:intergenic
ATGGCCACCACCAATAATGG+AGG 0.694001 2_4:+13604477 None:intergenic
TAAACGTGCCAAGACTACTG+AGG 0.716111 2_4:-13606482 Msa0316470:intron
AGATAAGTGATGGATGCCAA+TGG 0.716557 2_4:-13605374 Msa0316470:CDS
AGTCCAGAATCAGAAACACA+AGG 0.719780 2_4:-13605620 Msa0316470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATATATTTTTAAAAA+AGG + chr2_4:13606936-13606955 None:intergenic 0.0%
!! ATATATAATAAACAAACAAA+AGG + chr2_4:13604237-13604256 None:intergenic 10.0%
!!! AATATTTTATCATTGAATTT+TGG + chr2_4:13604629-13604648 None:intergenic 10.0%
!! AATTAATATTGTTAAAATTC+AGG - chr2_4:13605429-13605448 Msa0316470:intron 10.0%
!! AGAAATATGAGAAATTAATA+AGG + chr2_4:13604604-13604623 None:intergenic 15.0%
!! AATTAAATCACAATTGATTA+AGG - chr2_4:13605810-13605829 Msa0316470:CDS 15.0%
!!! ATTGATGTTTTTTTGTTAAT+AGG - chr2_4:13606202-13606221 Msa0316470:CDS 15.0%
!! TAATATTTATCTGAAAAACA+AGG + chr2_4:13606911-13606930 None:intergenic 15.0%
!! TAAATCCAATATCATCAAAA+AGG - chr2_4:13604520-13604539 Msa0316470:CDS 20.0%
!! TTTGTTCAAATATTATAGAC+TGG - chr2_4:13604692-13604711 Msa0316470:CDS 20.0%
!! GTTTATATATCAATTTCATC+TGG + chr2_4:13604905-13604924 None:intergenic 20.0%
!! TATGGAATTCAAATCTTAAA+TGG - chr2_4:13605189-13605208 Msa0316470:intron 20.0%
!!! TATAATCAGTCAAAATTTTC+TGG - chr2_4:13606599-13606618 Msa0316470:intron 20.0%
!!! ATTATTCCTTTTTTTGTACA+TGG - chr2_4:13606951-13606970 Msa0316470:CDS 20.0%
!!! TTATTCCTTTTTTTGTACAT+GGG - chr2_4:13606952-13606971 Msa0316470:CDS 20.0%
!!! TTTTTCACATTGAATAATCT+AGG - chr2_4:13607093-13607112 Msa0316470:five_prime_UTR 20.0%
! AAAGAGATTAGATATGTTGA+AGG + chr2_4:13604177-13604196 None:intergenic 25.0%
! TATATTATATGGACAAAGCA+TGG - chr2_4:13604252-13604271 Msa0316470:three_prime_UTR 25.0%
! ATATTATATGGACAAAGCAT+GGG - chr2_4:13604253-13604272 Msa0316470:three_prime_UTR 25.0%
!! GATAGAATGTTTCCTATTTT+AGG - chr2_4:13604371-13604390 Msa0316470:three_prime_UTR 25.0%
! TGATATTGGATTTAACATGT+TGG + chr2_4:13604514-13604533 None:intergenic 25.0%
!!! AATCTCCTTTTTGATGATAT+TGG + chr2_4:13604528-13604547 None:intergenic 25.0%
! ATTAACATGGATATTAGAAG+TGG + chr2_4:13604573-13604592 None:intergenic 25.0%
! TTAGTGTATGTTTGATATCA+CGG - chr2_4:13604820-13604839 Msa0316470:CDS 25.0%
! GACTTTGTAAACTCAAAATA+TGG - chr2_4:13605171-13605190 Msa0316470:intron 25.0%
! CAATCTGGCATATAATATAA+GGG + chr2_4:13605395-13605414 None:intergenic 25.0%
! TTATTAACATGTTACCAATC+TGG + chr2_4:13605410-13605429 None:intergenic 25.0%
!!! AATATTTTCTGAGACTTGTT+TGG + chr2_4:13605679-13605698 None:intergenic 25.0%
!! TTTCTAACTGAATTTTGTGA+TGG - chr2_4:13605857-13605876 Msa0316470:intron 25.0%
! ATATAATTGCAGATAAGTGA+TGG - chr2_4:13605882-13605901 Msa0316470:intron 25.0%
!!! CATTTGGACTATTTTTTTAG+TGG + chr2_4:13606054-13606073 None:intergenic 25.0%
! AATTTAGAAAAAGCGACATT+TGG + chr2_4:13606070-13606089 None:intergenic 25.0%
! ATAGCTCTTGCTAATAAATT+TGG + chr2_4:13606389-13606408 None:intergenic 25.0%
!! TTTGAAATTGCAATGGATTT+GGG + chr2_4:13606496-13606515 None:intergenic 25.0%
!! AATACAATTAGCAACTTTTC+AGG - chr2_4:13606857-13606876 Msa0316470:CDS 25.0%
! CTAAATATTCCTTTGTTCTT+AGG - chr2_4:13606883-13606902 Msa0316470:CDS 25.0%
!!! TCTTTTTAGTCCATATGTTT+TGG - chr2_4:13607001-13607020 Msa0316470:CDS 25.0%
!!! CTTTTTAGTCCATATGTTTT+GGG - chr2_4:13607002-13607021 Msa0316470:CDS 25.0%
TATTATATGGACAAAGCATG+GGG - chr2_4:13604254-13604273 Msa0316470:three_prime_UTR 30.0%
!!! GCTGTTTTGAAGGTAAAAAA+GGG + chr2_4:13604331-13604350 None:intergenic 30.0%
AATAGGAAACATTCTATCGT+TGG + chr2_4:13604369-13604388 None:intergenic 30.0%
TTGACAGGAAATCCTAAAAT+AGG + chr2_4:13604386-13604405 None:intergenic 30.0%
TAAGGAAGCTTACATTAACA+TGG + chr2_4:13604586-13604605 None:intergenic 30.0%
! TAATTACCTCATTTTCAGTG+TGG + chr2_4:13605077-13605096 None:intergenic 30.0%
TTCATGACATGTCAATTTAG+TGG - chr2_4:13605139-13605158 Msa0316470:intron 30.0%
TATACTAACATCTCATATGC+AGG + chr2_4:13605267-13605286 None:intergenic 30.0%
CCAATCTGGCATATAATATA+AGG + chr2_4:13605396-13605415 None:intergenic 30.0%
CCTTATATTATATGCCAGAT+TGG - chr2_4:13605393-13605412 Msa0316470:intron 30.0%
GAGGAAATATGGACAAAAAA+TGG - chr2_4:13605914-13605933 Msa0316470:intron 30.0%
TATGGACAAAAAATGGCTAA+AGG - chr2_4:13605921-13605940 Msa0316470:intron 30.0%
TTAGTGACGAAATTAGAGAT+AGG - chr2_4:13606089-13606108 Msa0316470:intron 30.0%
! ATCTTGGTAACAACATATGA+AGG - chr2_4:13606251-13606270 Msa0316470:CDS 30.0%
TGAGATCTAATGTAACAGTA+GGG + chr2_4:13606460-13606479 None:intergenic 30.0%
!! CTTTGAAATTGCAATGGATT+TGG + chr2_4:13606497-13606516 None:intergenic 30.0%
AAACTAGGTACTTGATGAAA+TGG + chr2_4:13606533-13606552 None:intergenic 30.0%
ACTTTCCCATGTACAAAAAA+AGG + chr2_4:13606960-13606979 None:intergenic 30.0%
AATACGAGAGAGTAAAGAGA+GGG + chr2_4:13604154-13604173 None:intergenic 35.0%
TGAGAGTGAGATATTGGAAT+TGG + chr2_4:13604308-13604327 None:intergenic 35.0%
!!! GGCTGTTTTGAAGGTAAAAA+AGG + chr2_4:13604332-13604351 None:intergenic 35.0%
TCGTTGGTATAAGTAGATGA+TGG + chr2_4:13604353-13604372 None:intergenic 35.0%
! TGTTGGTTGTGTTCATTTGA+AGG + chr2_4:13604497-13604516 None:intergenic 35.0%
ACATGGATATTAGAAGTGGA+AGG + chr2_4:13604569-13604588 None:intergenic 35.0%
TACTACAGATTGATCACTTC+CGG + chr2_4:13604735-13604754 None:intergenic 35.0%
GATCAATCTGTAGTAGATGA+TGG - chr2_4:13604740-13604759 Msa0316470:CDS 35.0%
TCATGTTACCGGTAATTACA+CGG - chr2_4:13605011-13605030 Msa0316470:CDS 35.0%
GGTTGTTAATGCAAAAACTG+AGG - chr2_4:13605450-13605469 Msa0316470:intron 35.0%
AAAACTGAGGAGAAAAACCA+AGG - chr2_4:13605463-13605482 Msa0316470:intron 35.0%
CCTATCTCAAGAAATTGTCT+TGG + chr2_4:13605508-13605527 None:intergenic 35.0%
CCAAGACAATTTCTTGAGAT+AGG - chr2_4:13605505-13605524 Msa0316470:CDS 35.0%
CTTGAGATAGGTACTACTAA+TGG - chr2_4:13605517-13605536 Msa0316470:CDS 35.0%
AGGAATAGCAATGGTAAAAC+TGG - chr2_4:13605613-13605632 Msa0316470:CDS 35.0%
ATAGCAATGGTAAAACTGGT+AGG - chr2_4:13605617-13605636 Msa0316470:CDS 35.0%
TAGCAATGGTAAAACTGGTA+GGG - chr2_4:13605618-13605637 Msa0316470:CDS 35.0%
ATTTGCTTGATCTGCAACAT+TGG + chr2_4:13605710-13605729 None:intergenic 35.0%
TTTGTCCATATTTCCTCCAT+TGG + chr2_4:13605911-13605930 None:intergenic 35.0%
AGCATACTATAGATGCACTA+TGG - chr2_4:13605959-13605978 Msa0316470:intron 35.0%
!! TACCAAGATTGTTTTGTCCT+CGG + chr2_4:13606240-13606259 None:intergenic 35.0%
TCTAATGTAACAGTAGGGAA+TGG + chr2_4:13606455-13606474 None:intergenic 35.0%
GTGAGATCTAATGTAACAGT+AGG + chr2_4:13606461-13606480 None:intergenic 35.0%
!! TGAGGTCTTTGAAATTGCAA+TGG + chr2_4:13606503-13606522 None:intergenic 35.0%
CAAGTACCTAGTTTCTTTCA+AGG - chr2_4:13606539-13606558 Msa0316470:CDS 35.0%
TTCTGTCCTTGAAAGAAACT+AGG + chr2_4:13606548-13606567 None:intergenic 35.0%
CTTCAATTGTCTCAAGAAGT+TGG - chr2_4:13606623-13606642 Msa0316470:intron 35.0%
! GTTGTTGTTGTTGTTGTTGT+TGG + chr2_4:13606679-13606698 None:intergenic 35.0%
! TGTTGTTGCTATTGTTTGCA+TGG + chr2_4:13606811-13606830 None:intergenic 35.0%
AACAAGGAACCTAAGAACAA+AGG + chr2_4:13606895-13606914 None:intergenic 35.0%
!!! TCCATATGTTTTGGGATTGA+GGG - chr2_4:13607010-13607029 Msa0316470:exon 35.0%
GAATACGAGAGAGTAAAGAG+AGG + chr2_4:13604155-13604174 None:intergenic 40.0%
AAAGGGTGAGAGTGAGATAT+TGG + chr2_4:13604314-13604333 None:intergenic 40.0%
!! GTAGATGATGGCTGTTTTGA+AGG + chr2_4:13604341-13604360 None:intergenic 40.0%
! AGAGAAGAAATCGACTTCAC+CGG + chr2_4:13604456-13604475 None:intergenic 40.0%
! AGAAGTGGAAGGCTTTTCAT+CGG + chr2_4:13604558-13604577 None:intergenic 40.0%
GGTTTACAACTTGCAAACAC+CGG - chr2_4:13604713-13604732 Msa0316470:CDS 40.0%
ATTACGTACCTCAGTAGTCT+TGG + chr2_4:13604795-13604814 None:intergenic 40.0%
AATTGCAAGTGGAGCTTCAA+CGG - chr2_4:13604950-13604969 Msa0316470:CDS 40.0%
GAGATGCTAAGTCATGTTAC+CGG - chr2_4:13605000-13605019 Msa0316470:CDS 40.0%
GAATCACCACACTGAAAATG+AGG - chr2_4:13605068-13605087 Msa0316470:intron 40.0%
AGTCCAGAATCAGAAACACA+AGG - chr2_4:13605646-13605665 Msa0316470:CDS 40.0%
AGAATCAGAAACACAAGGCT+GGG - chr2_4:13605651-13605670 Msa0316470:CDS 40.0%
AATGAGAAAAGCTCGTGTCT+CGG - chr2_4:13605741-13605760 Msa0316470:CDS 40.0%
TGCTAGATCAGAAGCTTCAA+TGG - chr2_4:13605768-13605787 Msa0316470:CDS 40.0%
!! AGATAAGTGATGGATGCCAA+TGG - chr2_4:13605892-13605911 Msa0316470:intron 40.0%
TATAGATGCACTATGGCAGT+CGG - chr2_4:13605966-13605985 Msa0316470:intron 40.0%
AAACTCACTTGTTTGCGAAC+TGG + chr2_4:13605996-13606015 None:intergenic 40.0%
TCTACCTTGTTCAACAAGCA+TGG - chr2_4:13606409-13606428 Msa0316470:intron 40.0%
TGATGAAATGGTGCATGTTG+AGG + chr2_4:13606521-13606540 None:intergenic 40.0%
! TGTTGTTGTTGTTGTTGGCT+TGG + chr2_4:13606674-13606693 None:intergenic 40.0%
AACAGCGGTGAAATTAGGAT+CGG + chr2_4:13606753-13606772 None:intergenic 40.0%
!!! GTCCATATGTTTTGGGATTG+AGG - chr2_4:13607009-13607028 Msa0316470:CDS 40.0%
!!! CCATATGTTTTGGGATTGAG+GGG - chr2_4:13607011-13607030 Msa0316470:exon 40.0%
AACCTTACCCGTAACACCAA+CGG - chr2_4:13604404-13604423 Msa0316470:CDS 45.0%
!! CGACTTCACCGGTTATTTTC+CGG + chr2_4:13604445-13604464 None:intergenic 45.0%
TAAACGTGCCAAGACTACTG+AGG - chr2_4:13604784-13604803 Msa0316470:CDS 45.0%
TCAGCTTGCACAATTGCAAG+TGG - chr2_4:13604939-13604958 Msa0316470:CDS 45.0%
CTGCAAAGCCGTGTAATTAC+CGG + chr2_4:13605022-13605041 None:intergenic 45.0%
GAGGAGAAAAACCAAGGTGT+TGG - chr2_4:13605469-13605488 Msa0316470:intron 45.0%
!! GAGAAAAACCAAGGTGTTGG+TGG - chr2_4:13605472-13605491 Msa0316470:intron 45.0%
GATCAAACACACCTCAGATG+AGG - chr2_4:13605593-13605612 Msa0316470:CDS 45.0%
! CCATTGCTATTCCTCATCTG+AGG + chr2_4:13605607-13605626 None:intergenic 45.0%
CCTCAGATGAGGAATAGCAA+TGG - chr2_4:13605604-13605623 Msa0316470:CDS 45.0%
! CAGCCTTGTGTTTCTGATTC+TGG + chr2_4:13605652-13605671 None:intergenic 45.0%
CAGAATCAGAAACACAAGGC+TGG - chr2_4:13605650-13605669 Msa0316470:CDS 45.0%
GAATCAGAAACACAAGGCTG+GGG - chr2_4:13605652-13605671 Msa0316470:CDS 45.0%
!! TAAGTGATGGATGCCAATGG+AGG - chr2_4:13605895-13605914 Msa0316470:intron 45.0%
GGATGCCAATGGAGGAAATA+TGG - chr2_4:13605903-13605922 Msa0316470:intron 45.0%
CTATAGTATGCTCGAGGACA+TGG + chr2_4:13605951-13605970 None:intergenic 45.0%
GTGCATCTATAGTATGCTCG+AGG + chr2_4:13605957-13605976 None:intergenic 45.0%
TGCCGAGGACAAAACAATCT+TGG - chr2_4:13606235-13606254 Msa0316470:CDS 45.0%
GCAGCAAGCATGTTACTATC+AGG - chr2_4:13606332-13606351 Msa0316470:intron 45.0%
GGATCAATGTCAAGTGCAGA+TGG - chr2_4:13606353-13606372 Msa0316470:intron 45.0%
! GTTGCCATGCTTGTTGAACA+AGG + chr2_4:13606416-13606435 None:intergenic 45.0%
ACAGCCTTCTTTAGCTGACT+CGG - chr2_4:13606700-13606719 Msa0316470:CDS 45.0%
GCTGCTATCTCCTCCATTAT+TGG - chr2_4:13606779-13606798 Msa0316470:CDS 45.0%
GCTATCTCCTCCATTATTGG+TGG - chr2_4:13606782-13606801 Msa0316470:CDS 45.0%
ATCTCCTCCATTATTGGTGG+TGG - chr2_4:13606785-13606804 Msa0316470:CDS 45.0%
CCCCTCAATCCCAAAACATA+TGG + chr2_4:13607014-13607033 None:intergenic 45.0%
!! TTTGTTTATTATATATTATA+TGG - chr2_4:13604241-13604260 Msa0316470:three_prime_UTR 5.0%
GAGAGAGTAAAGAGAGGGAG+AGG + chr2_4:13604149-13604168 None:intergenic 50.0%
GTGTTACGGGTAAGGTTGAC+AGG + chr2_4:13604401-13604420 None:intergenic 50.0%
GGCTTTGCAGATGCAACTTG+TGG - chr2_4:13605032-13605051 Msa0316470:CDS 50.0%
ATAGGTTCAACGTTGTGCCG+AGG - chr2_4:13606220-13606239 Msa0316470:CDS 50.0%
!! TAGCTGCTGGTGGTAATGGA+TGG + chr2_4:13606286-13606305 None:intergenic 50.0%
TCCATTACCACCAGCAGCTA+TGG - chr2_4:13606286-13606305 Msa0316470:CDS 50.0%
! TAACATGCTTGCTGCTGCTG+AGG + chr2_4:13606327-13606346 None:intergenic 50.0%
!! TTGTTGGCTTGGAGCTTGTG+TGG + chr2_4:13606663-13606682 None:intergenic 50.0%
GAGTCAGCTAAAGAAGGCTG+TGG + chr2_4:13606701-13606720 None:intergenic 50.0%
CTGACCGAGTCAGCTAAAGA+AGG + chr2_4:13606707-13606726 None:intergenic 50.0%
AGCGGTGAAATTAGGATCGG+CGG + chr2_4:13606750-13606769 None:intergenic 50.0%
GCGAGAACAGCGGTGAAATT+AGG + chr2_4:13606758-13606777 None:intergenic 50.0%
ATGGCCACCACCAATAATGG+AGG + chr2_4:13606792-13606811 None:intergenic 50.0%
TGCATGGCCACCACCAATAA+TGG + chr2_4:13606795-13606814 None:intergenic 50.0%
!! GTCTTCGCCGTTGGTGTTAC+GGG + chr2_4:13604414-13604433 None:intergenic 55.0%
!! CGTCTTCGCCGTTGGTGTTA+CGG + chr2_4:13604415-13604434 None:intergenic 55.0%
CGTAACACCAACGGCGAAGA+CGG - chr2_4:13604413-13604432 Msa0316470:CDS 55.0%
GGCGGTGACCGGAAAATAAC+CGG - chr2_4:13604434-13604453 Msa0316470:CDS 55.0%
CCATTGCTCCACCAACACCT+TGG + chr2_4:13605483-13605502 None:intergenic 55.0%
!! CCAAGGTGTTGGTGGAGCAA+TGG - chr2_4:13605480-13605499 Msa0316470:intron 55.0%
GCCATAGCTGCTGGTGGTAA+TGG + chr2_4:13606290-13606309 None:intergenic 55.0%
ACCACCAGCAGCTATGGCAA+TGG - chr2_4:13606292-13606311 Msa0316470:CDS 55.0%
GATGCCATTGCCATAGCTGC+TGG + chr2_4:13606299-13606318 None:intergenic 55.0%
GATAGCAGCTGCGAGAACAG+CGG + chr2_4:13606768-13606787 None:intergenic 55.0%
!! CGCCGTTGGTGTTACGGGTA+AGG + chr2_4:13604409-13604428 None:intergenic 60.0%
AACACCAACGGCGAAGACGG+CGG - chr2_4:13604416-13604435 Msa0316470:CDS 60.0%
GCCATTGCCATAGCTGCTGG+TGG + chr2_4:13606296-13606315 None:intergenic 60.0%
AGGATCGGCGGTGATAGCAG+CGG + chr2_4:13606738-13606757 None:intergenic 60.0%
ATCGGCGGTGATAGCAGCGG+TGG + chr2_4:13606735-13606754 None:intergenic 65.0%
GTCACCGCCGTCTTCGCCGT+TGG + chr2_4:13604423-13604442 None:intergenic 70.0%
ACGGCGAAGACGGCGGTGAC+CGG - chr2_4:13604423-13604442 Msa0316470:CDS 70.0%
GGCGGTGATAGCAGCGGTGG+CGG + chr2_4:13606732-13606751 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 13604142 13607146 13604142 ID=Msa0316470;Name=Msa0316470
chr2_4 mRNA 13604142 13607146 13604142 ID=Msa0316470-mRNA-1;Parent=Msa0316470;Name=Msa0316470-mRNA-1;_AED=0.08;_eAED=0.08;_QI=118|1|1|1|1|1|6|260|563
chr2_4 exon 13604142 13605064 13604142 ID=Msa0316470-mRNA-1:exon:5145;Parent=Msa0316470-mRNA-1
chr2_4 exon 13605281 13605394 13605281 ID=Msa0316470-mRNA-1:exon:5144;Parent=Msa0316470-mRNA-1
chr2_4 exon 13605499 13605837 13605499 ID=Msa0316470-mRNA-1:exon:5143;Parent=Msa0316470-mRNA-1
chr2_4 exon 13606199 13606345 13606199 ID=Msa0316470-mRNA-1:exon:5142;Parent=Msa0316470-mRNA-1
chr2_4 exon 13606483 13606578 13606483 ID=Msa0316470-mRNA-1:exon:5141;Parent=Msa0316470-mRNA-1
chr2_4 exon 13606696 13607146 13606696 ID=Msa0316470-mRNA-1:exon:5140;Parent=Msa0316470-mRNA-1
chr2_4 five_prime_UTR 13607029 13607146 13607029 ID=Msa0316470-mRNA-1:five_prime_utr;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13606696 13607028 13606696 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13606483 13606578 13606483 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13606199 13606345 13606199 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13605499 13605837 13605499 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13605281 13605394 13605281 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 CDS 13604402 13605064 13604402 ID=Msa0316470-mRNA-1:cds;Parent=Msa0316470-mRNA-1
chr2_4 three_prime_UTR 13604142 13604401 13604142 ID=Msa0316470-mRNA-1:three_prime_utr;Parent=Msa0316470-mRNA-1
Gene Sequence

>Msa0316470

ATGGACAAAGCATGGGGTCTAGCTCTTGATGCTTCTTCATCTTCTCCAATTCCAATATCTCACTCTCACCCTTTTTTACCTTCAAAACAGCCATCATCTACTTATACCAACGATAGAATGTTTCCTATTTTAGGATTTCCTGTCAACCTTACCCGTAACACCAACGGCGAAGACGGCGGTGACCGGAAAATAACCGGTGAAGTCGATTTCTTCTCTGAAAGAAACAAACTATCACCTTCAAATGAACACAACCAACATGTTAAATCCAATATCATCAAAAAGGAGATTGTTTCAACCGATGAAAAGCCTTCCACTTCTAATATCCATGTTAATACTGGTTTACAACTTGCAAACACCGGAAGTGATCAATCTGTAGTAGATGATGGAGTTACATCTGATGCTGAAAATAAACGTGCCAAGACTACTGAGCTTGCACAATTGCAAGTGGAGCTTCAACGGATGAATTCAGAGAACAAAAAGCTGAAAGAGATGCTAAGTCATGTTACCGGTAATTACACGGCTTTGCAGATGCAACTTGTGGCATTAATGCAAAAGAATCACCACACTGAAAATGAGGTTGTTAATGCAAAAACTGAGGAGAAAAACCAAGGTGTTGGTGGAGCAATGGTACCAAGACAATTTCTTGAGATAGGTACTACTAATGGAACAGCAGAAGTTGAAGATCAAGTATCAAACTCATCATCTGATGAAAGAACAAGATCAAACACACCTCAGATGAGGAATAGCAATGGTAAAACTGGTAGGGAAGACAGTCCAGAATCAGAAACACAAGGCTGGGGACCAAACAAGTCTCAGAAAATATTGAACTCTTCCAATGTTGCAGATCAAGCAAATACAGAAGCTACAATGAGAAAAGCTCGTGTCTCGGTTCGTGCTAGATCAGAAGCTTCAATGATAAGTGATGGATGCCAATGGAGGAAATATGGACAAAAAATGGCTAAAGGAAATCCATGTCCTCGAGCATACTATAGATGCACTATGGCAGTCGGTTGTCCAGTTCGCAAACAAGTTCAACGTTGTGCCGAGGACAAAACAATCTTGGTAACAACATATGAAGGAACACATAACCATCCATTACCACCAGCAGCTATGGCAATGGCATCAACAACCTCAGCAGCAGCAAGCATGTTACTATCAGGATCAATGTCAAGTGCAGATGGAATAATGACACCAAATTTATTAGCAAGAGCTATTCTACCTTGTTCAACAAGCATGGCAACACTTTCAGCTTCAGCACCATTCCCTACTGTTACATTAGATCTCACACAAAATTCAAACCCAAATCCATTGCAATTTCAAAGACCTCAACATGCACCATTTCATCAAGTACCTAGTTTCTTTCAAGGACAGAATCAGAATTTTGCACAAGCAGCAGCATCACTTTATAATCAGTCAAAATTTTCTGGTCTTCAATTGTCTCAAGAAGTTGGTTCTTCTCATTTAACCACACAAGCTCCAAGCCAACAACAACAACAACAACAACCACAGCCTTCTTTAGCTGACTCGGTCAGCGCCGCCACCGCTGCTATCACCGCCGATCCTAATTTCACCGCTGTTCTCGCAGCTGCTATCTCCTCCATTATTGGTGGTGGCCATGCAAACAATAGCAACAACAGCAACAACAACAACAGCAATAGCAGAAATACAATTAGCAACTTTTCAGGAAACTAA
Protein sequence

>Msa0316470

MDKAWGLALDASSSSPIPISHSHPFLPSKQPSSTYTNDRMFPILGFPVNLTRNTNGEDGGDRKITGEVDFFSERNKLSPSNEHNQHVKSNIIKKEIVSTDEKPSTSNIHVNTGLQLANTGSDQSVVDDGVTSDAENKRAKTTELAQLQVELQRMNSENKKLKEMLSHVTGNYTALQMQLVALMQKNHHTENEVVNAKTEEKNQGVGGAMVPRQFLEIGTTNGTAEVEDQVSNSSSDERTRSNTPQMRNSNGKTGREDSPESETQGWGPNKSQKILNSSNVADQANTEATMRKARVSVRARSEASMISDGCQWRKYGQKMAKGNPCPRAYYRCTMAVGCPVRKQVQRCAEDKTILVTTYEGTHNHPLPPAAMAMASTTSAAASMLLSGSMSSADGIMTPNLLARAILPCSTSMATLSASAPFPTVTLDLTQNSNPNPLQFQRPQHAPFHQVPSFFQGQNQNFAQAAASLYNQSKFSGLQLSQEVGSSHLTTQAPSQQQQQQQPQPSLADSVSAATAAITADPNFTAVLAAAISSIIGGGHANNSNNSNNNNSNSRNTISNFSGN*