AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0771550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771550 MtrunA17_Chr5g0425071 96.933 489 15 0 1 489 1 489 0.0 984
Msa0771550 MtrunA17_Chr1g0194391 51.738 489 225 8 5 488 54 536 1.69e-170 491
Msa0771550 MtrunA17_Chr5g0443341 49.895 475 219 8 15 480 64 528 6.00e-158 459
Msa0771550 MtrunA17_Chr3g0096481 49.167 480 226 9 15 485 59 529 2.56e-154 449
Msa0771550 MtrunA17_Chr4g0005991 42.505 487 265 8 10 487 15 495 1.67e-121 364
Msa0771550 MtrunA17_Chr8g0348781 42.593 486 257 8 8 479 18 495 1.48e-120 362
Msa0771550 MtrunA17_Chr1g0170421 40.404 495 275 10 8 487 45 534 7.13e-119 361
Msa0771550 MtrunA17_Chr7g0241831 39.877 489 288 4 2 486 10 496 3.17e-116 351
Msa0771550 MtrunA17_Chr5g0435581 40.042 477 269 8 17 482 3 473 9.12e-116 350
Msa0771550 MtrunA17_Chr3g0096591 55.678 273 114 3 210 479 1 269 6.72e-109 324
Msa0771550 MtrunA17_Chr3g0096581 40.972 144 83 2 15 157 63 205 8.70e-30 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0771550 AT4G01950.1 51.653 484 221 10 11 484 40 520 1.87e-169 488
Msa0771550 AT4G01950.2 51.653 484 221 10 11 484 68 548 5.08e-169 488
Msa0771550 AT1G02390.1 50.000 490 222 8 8 480 43 526 2.92e-164 475
Msa0771550 AT1G06520.1 47.942 486 224 11 17 480 105 583 5.63e-150 441
Msa0771550 AT2G38110.1 42.437 476 259 9 14 479 22 492 6.59e-119 358
Msa0771550 AT3G11430.1 39.959 483 267 9 14 482 9 482 2.02e-118 357
Msa0771550 AT5G06090.1 40.329 486 271 12 9 482 2 480 5.49e-118 356
Msa0771550 AT1G01610.1 42.171 479 264 8 17 485 23 498 4.97e-115 348
Msa0771550 AT4G00400.1 41.525 472 263 7 17 479 23 490 2.96e-109 333
Msa0771550 AT3G11325.2 32.104 461 213 9 26 482 9 373 2.18e-71 232
Msa0771550 AT3G11325.1 32.104 461 213 9 26 482 6 370 2.40e-71 232

Find 93 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCTTGAAAGACTATTT+AGG 0.195935 5_2:-44412144 Msa0771550:CDS
TGTAATGAGGGAAAAGTTTC+TGG 0.203098 5_2:+44411968 None:intergenic
GTATAAGAAAAGACACTTTA+AGG 0.207106 5_2:-44412287 Msa0771550:CDS
GAGAATAGTATTGGTATTAT+TGG 0.218296 5_2:-44412012 Msa0771550:CDS
TCAACTAGGTTTGAAGATTA+TGG 0.218722 5_2:-44412328 Msa0771550:CDS
ATTAAAACTAAGATAGAAAT+AGG 0.231887 5_2:+44410321 None:intergenic
GAAGGACAAATATATGATTT+TGG 0.246526 5_2:-44410444 Msa0771550:CDS
AAACCTTCAACCATTATTCT+TGG 0.248292 5_2:+44412162 None:intergenic
AATTGTAGTTATACTTGTTT+TGG 0.249787 5_2:+44412585 None:intergenic
TTAACAAAGTAAACAGGATT+TGG 0.272628 5_2:+44410593 None:intergenic
AAATGAAATTGGAAAGAGTT+TGG 0.276403 5_2:-44410492 Msa0771550:CDS
GTCCCTGAAAATGCTAATAT+TGG 0.280666 5_2:+44411049 None:intergenic
GTGAAGTACACAATATTATA+AGG 0.281883 5_2:-44410097 Msa0771550:three_prime_UTR
GGCTTAGGAAACAACTTTCT+TGG 0.284978 5_2:+44411214 None:intergenic
TCTCTACATGTTGTTCCTTC+TGG 0.291482 5_2:+44410761 None:intergenic
ATTGGAGCTGATGCATTAAA+AGG 0.293102 5_2:+44411067 None:intergenic
TCTGGTCAATATACTCAATA+TGG 0.319173 5_2:+44411986 None:intergenic
AATGAAATTGGAAAGAGTTT+GGG 0.331774 5_2:-44410491 Msa0771550:CDS
TTTGTTTCTTTCATACCCTT+TGG 0.336221 5_2:-44412370 Msa0771550:CDS
ACATCACAAACATGTCAAAA+AGG 0.340009 5_2:-44410551 Msa0771550:CDS
TTTGTCCTTCCTAGTCAAAT+TGG 0.343154 5_2:+44410457 None:intergenic
GTTCTGATGAGAGGAGAAAT+TGG 0.353548 5_2:-44411246 Msa0771550:CDS
GCGGATCAAGCAAAGTTCTA+TGG 0.355996 5_2:+44410951 None:intergenic
GTTTCTTGAAAGACTATTTA+GGG 0.373176 5_2:-44412143 Msa0771550:CDS
TACCCTTTGGTATGGTTAGT+TGG 0.374543 5_2:-44412357 Msa0771550:CDS
TCCTGTTGCTGTTGATGTTA+AGG 0.382374 5_2:-44410684 Msa0771550:CDS
TGACACACAAATTAACTTGT+AGG 0.389095 5_2:+44410361 None:intergenic
GCTTGTTGCTTTAACATCAT+AGG 0.399072 5_2:+44412038 None:intergenic
ATTGGAAAGAGTTTGGGATT+TGG 0.401189 5_2:-44410485 Msa0771550:CDS
TATAAGAAAAGACACTTTAA+GGG 0.412950 5_2:-44412286 Msa0771550:CDS
TTCTTTCATACCCTTTGGTA+TGG 0.423298 5_2:-44412365 Msa0771550:CDS
CGCGCGACCGAGCAAGAGAT+AGG 0.423610 5_2:-44410829 Msa0771550:CDS
TAGTAAGTTACCAAGAATAA+TGG 0.425255 5_2:-44412172 Msa0771550:CDS
ACCTTAACATCAACAGCAAC+AGG 0.427108 5_2:+44410683 None:intergenic
GGACTAAACCTTAGCAAGTA+AGG 0.432275 5_2:+44410737 None:intergenic
CATCTTGCTGCAGGTAAGGT+TGG 0.435384 5_2:+44412542 None:intergenic
GCAAAATTAGCAACTTCAAC+AGG 0.437926 5_2:+44410518 None:intergenic
ATGCTAGTTTCCTTTGAAGC+TGG 0.441903 5_2:-44412414 Msa0771550:CDS
CTACTTAGAAGATGTTGGTT+GGG 0.444813 5_2:-44412238 Msa0771550:CDS
AGCTAGTGGATATAAATGTT+TGG 0.447892 5_2:-44410639 Msa0771550:CDS
TTACCAAGAATAATGGTTGA+AGG 0.459458 5_2:-44412165 Msa0771550:CDS
TCATGGAAAATCAATGGCTT+AGG 0.460797 5_2:+44411199 None:intergenic
AAGGAAACTAGCATGAAGTA+AGG 0.462736 5_2:+44412423 None:intergenic
TTAGAAGATGTTGGTTGGGA+AGG 0.468636 5_2:-44412234 Msa0771550:CDS
TGAAGTACACAATATTATAA+GGG 0.477556 5_2:-44410096 Msa0771550:three_prime_UTR
ACATCTTCTAAGTAGAACTT+TGG 0.478280 5_2:+44412246 None:intergenic
AGCAAGAGATAGGGATTCAA+TGG 0.481585 5_2:-44410819 Msa0771550:CDS
CTAGTTTCCTTTGAAGCTGG+TGG 0.484232 5_2:-44412411 Msa0771550:CDS
CTTCCATCATGGAAAATCAA+TGG 0.487517 5_2:+44411193 None:intergenic
TGCTTTAACATCATAGGAAG+TGG 0.493418 5_2:+44412044 None:intergenic
TAGTATTCCACCAGCTTCAA+AGG 0.493486 5_2:+44412404 None:intergenic
ACAAGCAAAGAGAATAGTAT+TGG 0.508669 5_2:-44412021 Msa0771550:CDS
GAAGTAAGGGAAGAGTGAAA+TGG 0.512705 5_2:+44412437 None:intergenic
TCTACTTAGAAGATGTTGGT+TGG 0.515294 5_2:-44412239 Msa0771550:CDS
CACCAACTAACCATACCAAA+GGG 0.528093 5_2:+44412355 None:intergenic
AGACACTTTAAGGGTAGGAA+TGG 0.534443 5_2:-44412277 Msa0771550:CDS
TTGAATCCCTATCTCTTGCT+CGG 0.535854 5_2:+44410822 None:intergenic
AGAAACCAAACCTTGTAATG+AGG 0.540345 5_2:+44411955 None:intergenic
GTAGAGAGCCTTACTTGCTA+AGG 0.541715 5_2:-44410745 Msa0771550:CDS
TGAAAGCCAATCTTCCATCA+TGG 0.549956 5_2:+44411182 None:intergenic
GAAACCAAACCTTGTAATGA+GGG 0.554140 5_2:+44411956 None:intergenic
AAGTTCTACTTAGAAGATGT+TGG 0.556652 5_2:-44412243 Msa0771550:CDS
GCGCGACCGAGCAAGAGATA+GGG 0.556887 5_2:-44410828 Msa0771550:CDS
ATGGAGAAAATGCTTTGTGA+AGG 0.565042 5_2:-44410800 Msa0771550:CDS
TTGCACCAATTTGACTAGGA+AGG 0.565389 5_2:-44410462 Msa0771550:CDS
TTATGTTAAGTTCTGATGAG+AGG 0.571837 5_2:-44411255 Msa0771550:CDS
AACCTTGTGGTATGTCCAGA+AGG 0.576105 5_2:-44410776 Msa0771550:CDS
AGTTATGAATATTACCTGGT+GGG 0.579341 5_2:-44410295 Msa0771550:three_prime_UTR
AGAAAAGACACTTTAAGGGT+AGG 0.579822 5_2:-44412282 Msa0771550:CDS
AGGTTGGTTCTTTAAAACAA+AGG 0.587296 5_2:+44412558 None:intergenic
ATGTTCATGTGGTTACCACT+TGG 0.589495 5_2:-44411133 Msa0771550:CDS
AGGAAACTAGCATGAAGTAA+GGG 0.591307 5_2:+44412424 None:intergenic
TTAAAGTTATGAATATTACC+TGG 0.596125 5_2:-44410299 Msa0771550:three_prime_UTR
TCACCAACTAACCATACCAA+AGG 0.596402 5_2:+44412354 None:intergenic
ATAGCTAAAAGTAAAATCAA+AGG 0.598704 5_2:-44410129 Msa0771550:three_prime_UTR
ATTGAAAGAAATAGTCCAAG+TGG 0.598959 5_2:+44411118 None:intergenic
AACTTGCAGTTATTATGACA+AGG 0.600625 5_2:+44410385 None:intergenic
TTAGTTGGTGAGTATCAACT+AGG 0.603020 5_2:-44412342 Msa0771550:CDS
TTGGTTGCACCAATTTGACT+AGG 0.608715 5_2:-44410466 Msa0771550:CDS
ACTAGTGAAGATGTTACAGT+TGG 0.610755 5_2:+44411157 None:intergenic
CTTCACTAGTCATGTTCATG+TGG 0.612621 5_2:-44411144 Msa0771550:CDS
AAGTTATGAATATTACCTGG+TGG 0.618592 5_2:-44410296 Msa0771550:three_prime_UTR
ACTTTGTTAAAATACTTGAG+AGG 0.622036 5_2:-44410580 Msa0771550:CDS
ATAGTGTGTGATATATAAAG+CGG 0.628520 5_2:+44410932 None:intergenic
TTCCTTCTGGACATACCACA+AGG 0.637353 5_2:+44410774 None:intergenic
TTTCTTGAAAGACTATTTAG+GGG 0.640361 5_2:-44412142 Msa0771550:CDS
CACCACACTACTGACCCACC+AGG 0.646644 5_2:+44410281 None:intergenic
TCAGTTAGCTCAGCAAACAA+AGG 0.654655 5_2:+44410716 None:intergenic
AGAGACATAAAGTCATACAA+TGG 0.657033 5_2:-44412102 Msa0771550:CDS
CTATAAGTGACTGCTGAGAG+TGG 0.658106 5_2:+44410902 None:intergenic
TACCTGGTGGGTCAGTAGTG+TGG 0.678698 5_2:-44410283 Msa0771550:three_prime_UTR
GGTGGGTCAGTAGTGTGGTG+AGG 0.711279 5_2:-44410278 Msa0771550:three_prime_UTR
GCTTTGTGAAGGAAACCTTG+TGG 0.758293 5_2:-44410789 Msa0771550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAAAAATTGTAATAATTT+TGG - chr5_2:44412489-44412508 Msa0771550:CDS 10.0%
!! ATTAAAACTAAGATAGAAAT+AGG + chr5_2:44412385-44412404 None:intergenic 15.0%
!!! ATAATTTTGGATGTTTATAT+AGG - chr5_2:44412502-44412521 Msa0771550:CDS 15.0%
!!! AATTGTAGTTATACTTGTTT+TGG + chr5_2:44410121-44410140 None:intergenic 20.0%
!! TATAAGAAAAGACACTTTAA+GGG - chr5_2:44410417-44410436 Msa0771550:CDS 20.0%
!! TGTTTATACTTTCAAAATCA+TGG - chr5_2:44410789-44410808 Msa0771550:CDS 20.0%
!!! TTTTAGACAACAAAAAAAGT+TGG + chr5_2:44411379-44411398 None:intergenic 20.0%
!!! TCAATTTTCAGAATAACATT+TGG - chr5_2:44411603-44411622 Msa0771550:intron 20.0%
!!! CTTTTGATTTTTTTCGTTTT+CGG + chr5_2:44411712-44411731 None:intergenic 20.0%
!!! AGTATTTTAACAAAGTAAAC+AGG + chr5_2:44412119-44412138 None:intergenic 20.0%
!! TTAAAGTTATGAATATTACC+TGG - chr5_2:44412404-44412423 Msa0771550:CDS 20.0%
!! ATAGCTAAAAGTAAAATCAA+AGG - chr5_2:44412574-44412593 Msa0771550:five_prime_UTR 20.0%
!!! TGAAAGTTTAGAAGTTTTGT+TGG + chr5_2:44410203-44410222 None:intergenic 25.0%
!!! ACACTAGTTTTTGATTTTGA+AGG - chr5_2:44410229-44410248 Msa0771550:three_prime_UTR 25.0%
!! ATGGTTTTCTTAACCTTTTT+TGG - chr5_2:44410394-44410413 Msa0771550:exon 25.0%
!! GTCTTTTCTTATACCAAAAA+AGG + chr5_2:44410410-44410429 None:intergenic 25.0%
! GTATAAGAAAAGACACTTTA+AGG - chr5_2:44410416-44410435 Msa0771550:CDS 25.0%
! TAGTAAGTTACCAAGAATAA+TGG - chr5_2:44410531-44410550 Msa0771550:CDS 25.0%
! GTTTCTTGAAAGACTATTTA+GGG - chr5_2:44410560-44410579 Msa0771550:CDS 25.0%
! TTTCTTGAAAGACTATTTAG+GGG - chr5_2:44410561-44410580 Msa0771550:CDS 25.0%
!!! TCATACAATGGTTACTTTTT+GGG - chr5_2:44410613-44410632 Msa0771550:CDS 25.0%
!!! GAGAATAGTATTGGTATTAT+TGG - chr5_2:44410691-44410710 Msa0771550:CDS 25.0%
!! AATGTTTTACAAAGAAAACG+AGG + chr5_2:44411170-44411189 None:intergenic 25.0%
! ATAGTGTGTGATATATAAAG+CGG + chr5_2:44411774-44411793 None:intergenic 25.0%
!! GTTAAGGTTAGTATGTTTTA+TGG - chr5_2:44412035-44412054 Msa0771550:CDS 25.0%
! TTAACAAAGTAAACAGGATT+TGG + chr5_2:44412113-44412132 None:intergenic 25.0%
! ACTTTGTTAAAATACTTGAG+AGG - chr5_2:44412123-44412142 Msa0771550:CDS 25.0%
!! AATTTTGCGCAAAATGAAAT+TGG - chr5_2:44412200-44412219 Msa0771550:CDS 25.0%
! AAATGAAATTGGAAAGAGTT+TGG - chr5_2:44412211-44412230 Msa0771550:CDS 25.0%
! AATGAAATTGGAAAGAGTTT+GGG - chr5_2:44412212-44412231 Msa0771550:CDS 25.0%
!! GAAGGACAAATATATGATTT+TGG - chr5_2:44412259-44412278 Msa0771550:CDS 25.0%
! GTGAAGTACACAATATTATA+AGG - chr5_2:44412606-44412625 Msa0771550:five_prime_UTR 25.0%
!! AGGTTGGTTCTTTAAAACAA+AGG + chr5_2:44410148-44410167 None:intergenic 30.0%
! TTTGTTTCTTTCATACCCTT+TGG - chr5_2:44410333-44410352 Msa0771550:three_prime_UTR 30.0%
TCAACTAGGTTTGAAGATTA+TGG - chr5_2:44410375-44410394 Msa0771550:three_prime_UTR 30.0%
ACATCTTCTAAGTAGAACTT+TGG + chr5_2:44410460-44410479 None:intergenic 30.0%
AAGTTCTACTTAGAAGATGT+TGG - chr5_2:44410460-44410479 Msa0771550:CDS 30.0%
TTACCAAGAATAATGGTTGA+AGG - chr5_2:44410538-44410557 Msa0771550:CDS 30.0%
AAACCTTCAACCATTATTCT+TGG + chr5_2:44410544-44410563 None:intergenic 30.0%
GGTTTCTTGAAAGACTATTT+AGG - chr5_2:44410559-44410578 Msa0771550:CDS 30.0%
AGAGACATAAAGTCATACAA+TGG - chr5_2:44410601-44410620 Msa0771550:CDS 30.0%
!!! GTCATACAATGGTTACTTTT+TGG - chr5_2:44410612-44410631 Msa0771550:CDS 30.0%
ACAAGCAAAGAGAATAGTAT+TGG - chr5_2:44410682-44410701 Msa0771550:CDS 30.0%
TCTGGTCAATATACTCAATA+TGG + chr5_2:44410720-44410739 None:intergenic 30.0%
!!! ACAAGGTTTGGTTTCTTTTT+TGG - chr5_2:44410755-44410774 Msa0771550:CDS 30.0%
TGTTTATAGTCGCTAATCAA+CGG + chr5_2:44410917-44410936 None:intergenic 30.0%
TGACACAAAAACCAAAACAA+CGG + chr5_2:44411144-44411163 None:intergenic 30.0%
! TTATGTTAAGTTCTGATGAG+AGG - chr5_2:44411448-44411467 Msa0771550:intron 30.0%
ATTGAAAGAAATAGTCCAAG+TGG + chr5_2:44411588-44411607 None:intergenic 30.0%
! CTCCAATATTAGCATTTTCA+GGG - chr5_2:44411652-44411671 Msa0771550:intron 30.0%
!!! TTAATCGCGTTGTTTTAATG+GGG + chr5_2:44411854-44411873 None:intergenic 30.0%
!!! GTTAATCGCGTTGTTTTAAT+GGG + chr5_2:44411855-44411874 None:intergenic 30.0%
! AGCTAGTGGATATAAATGTT+TGG - chr5_2:44412064-44412083 Msa0771550:CDS 30.0%
GGATTCAAGTAATGAAAAAC+AGG + chr5_2:44412092-44412111 None:intergenic 30.0%
! TTTTGACATGTTTGTGATGT+TGG + chr5_2:44412152-44412171 None:intergenic 30.0%
ACATCACAAACATGTCAAAA+AGG - chr5_2:44412152-44412171 Msa0771550:CDS 30.0%
!! GACAAATATATGATTTTGGC+AGG - chr5_2:44412263-44412282 Msa0771550:CDS 30.0%
AACTTGCAGTTATTATGACA+AGG + chr5_2:44412321-44412340 None:intergenic 30.0%
!! TGACACACAAATTAACTTGT+AGG + chr5_2:44412345-44412364 None:intergenic 30.0%
AAGTTATGAATATTACCTGG+TGG - chr5_2:44412407-44412426 Msa0771550:CDS 30.0%
AGTTATGAATATTACCTGGT+GGG - chr5_2:44412408-44412427 Msa0771550:CDS 30.0%
!!! AAGTTTTGTTGGCAAGTGAA+AGG + chr5_2:44410192-44410211 None:intergenic 35.0%
AGGAAACTAGCATGAAGTAA+GGG + chr5_2:44410282-44410301 None:intergenic 35.0%
AAGGAAACTAGCATGAAGTA+AGG + chr5_2:44410283-44410302 None:intergenic 35.0%
TTCTTTCATACCCTTTGGTA+TGG - chr5_2:44410338-44410357 Msa0771550:three_prime_UTR 35.0%
!! TTAGTTGGTGAGTATCAACT+AGG - chr5_2:44410361-44410380 Msa0771550:three_prime_UTR 35.0%
! AGAAAAGACACTTTAAGGGT+AGG - chr5_2:44410421-44410440 Msa0771550:CDS 35.0%
TCTACTTAGAAGATGTTGGT+TGG - chr5_2:44410464-44410483 Msa0771550:CDS 35.0%
! CTACTTAGAAGATGTTGGTT+GGG - chr5_2:44410465-44410484 Msa0771550:CDS 35.0%
TGCTTTAACATCATAGGAAG+TGG + chr5_2:44410662-44410681 None:intergenic 35.0%
!! GCTTGTTGCTTTAACATCAT+AGG + chr5_2:44410668-44410687 None:intergenic 35.0%
TGTAATGAGGGAAAAGTTTC+TGG + chr5_2:44410738-44410757 None:intergenic 35.0%
! GAAACTTTTCCCTCATTACA+AGG - chr5_2:44410738-44410757 Msa0771550:CDS 35.0%
! TTTTCCCTCATTACAAGGTT+TGG - chr5_2:44410743-44410762 Msa0771550:CDS 35.0%
GAAACCAAACCTTGTAATGA+GGG + chr5_2:44410750-44410769 None:intergenic 35.0%
AGAAACCAAACCTTGTAATG+AGG + chr5_2:44410751-44410770 None:intergenic 35.0%
TGCATTATTGTCTGATTTCG+CGG - chr5_2:44410994-44411013 Msa0771550:CDS 35.0%
GATTTCGCGGTTTAATACTA+CGG - chr5_2:44411007-44411026 Msa0771550:CDS 35.0%
! TTTTTCTCTCTCCGTTGTTT+TGG - chr5_2:44411130-44411149 Msa0771550:CDS 35.0%
ATACTGTGATTCCTGATTGT+AGG - chr5_2:44411286-44411305 Msa0771550:intron 35.0%
TACTGTGATTCCTGATTGTA+GGG - chr5_2:44411287-44411306 Msa0771550:intron 35.0%
TCATGGAAAATCAATGGCTT+AGG + chr5_2:44411507-44411526 None:intergenic 35.0%
!!! AAGCCATTGATTTTCCATGA+TGG - chr5_2:44411507-44411526 Msa0771550:intron 35.0%
CTTCCATCATGGAAAATCAA+TGG + chr5_2:44411513-44411532 None:intergenic 35.0%
!! GATTTTCCATGATGGAAGAT+TGG - chr5_2:44411515-44411534 Msa0771550:intron 35.0%
ACTAGTGAAGATGTTACAGT+TGG + chr5_2:44411549-44411568 None:intergenic 35.0%
ATTGGAGCTGATGCATTAAA+AGG + chr5_2:44411639-44411658 None:intergenic 35.0%
! GCTCCAATATTAGCATTTTC+AGG - chr5_2:44411651-44411670 Msa0771550:intron 35.0%
GTCCCTGAAAATGCTAATAT+TGG + chr5_2:44411657-44411676 None:intergenic 35.0%
!!! ATTTTTTTCGTTTTCGGTCG+AGG + chr5_2:44411706-44411725 None:intergenic 35.0%
!!! TAATCGCGTTGTTTTAATGG+GGG + chr5_2:44411853-44411872 None:intergenic 35.0%
!!! CGTTAATCGCGTTGTTTTAA+TGG + chr5_2:44411856-44411875 None:intergenic 35.0%
ATGGAGAAAATGCTTTGTGA+AGG - chr5_2:44411903-44411922 Msa0771550:intron 35.0%
! TTTTATGGAACAACAGCTAG+TGG - chr5_2:44412050-44412069 Msa0771550:CDS 35.0%
TCACAAACATGTCAAAAAGG+TGG - chr5_2:44412155-44412174 Msa0771550:CDS 35.0%
GCAAAATTAGCAACTTCAAC+AGG + chr5_2:44412188-44412207 None:intergenic 35.0%
! ATTGGAAAGAGTTTGGGATT+TGG - chr5_2:44412218-44412237 Msa0771550:CDS 35.0%
TTTGTCCTTCCTAGTCAAAT+TGG + chr5_2:44412249-44412268 None:intergenic 35.0%
!! ATGATTTTGGCAGGTAATGA+AGG - chr5_2:44412272-44412291 Msa0771550:CDS 35.0%
! TTTTCATCTTGCTGCAGGTA+AGG + chr5_2:44410168-44410187 None:intergenic 40.0%
! AAGGTTTTTCATCTTGCTGC+AGG + chr5_2:44410173-44410192 None:intergenic 40.0%
GAAGTAAGGGAAGAGTGAAA+TGG + chr5_2:44410269-44410288 None:intergenic 40.0%
!! ATGCTAGTTTCCTTTGAAGC+TGG - chr5_2:44410289-44410308 Msa0771550:three_prime_UTR 40.0%
TAGTATTCCACCAGCTTCAA+AGG + chr5_2:44410302-44410321 None:intergenic 40.0%
TACCCTTTGGTATGGTTAGT+TGG - chr5_2:44410346-44410365 Msa0771550:three_prime_UTR 40.0%
CACCAACTAACCATACCAAA+GGG + chr5_2:44410351-44410370 None:intergenic 40.0%
TCACCAACTAACCATACCAA+AGG + chr5_2:44410352-44410371 None:intergenic 40.0%
! AGACACTTTAAGGGTAGGAA+TGG - chr5_2:44410426-44410445 Msa0771550:CDS 40.0%
! TTAGAAGATGTTGGTTGGGA+AGG - chr5_2:44410469-44410488 Msa0771550:CDS 40.0%
TCAGAGCTTCAGAAAAACGA+GGG + chr5_2:44410858-44410877 None:intergenic 40.0%
TTCAGAGCTTCAGAAAAACG+AGG + chr5_2:44410859-44410878 None:intergenic 40.0%
TATAGTCGCTAATCAACGGA+AGG + chr5_2:44410913-44410932 None:intergenic 40.0%
GTTCTGATGAGAGGAGAAAT+TGG - chr5_2:44411457-44411476 Msa0771550:intron 40.0%
GGCTTAGGAAACAACTTTCT+TGG + chr5_2:44411492-44411511 None:intergenic 40.0%
TGAAAGCCAATCTTCCATCA+TGG + chr5_2:44411524-44411543 None:intergenic 40.0%
CTTCACTAGTCATGTTCATG+TGG - chr5_2:44411559-44411578 Msa0771550:intron 40.0%
ATGTTCATGTGGTTACCACT+TGG - chr5_2:44411570-44411589 Msa0771550:intron 40.0%
TTGAATCCCTATCTCTTGCT+CGG + chr5_2:44411884-44411903 None:intergenic 40.0%
AGCAAGAGATAGGGATTCAA+TGG - chr5_2:44411884-44411903 Msa0771550:intron 40.0%
TCTCTACATGTTGTTCCTTC+TGG + chr5_2:44411945-44411964 None:intergenic 40.0%
GGACTAAACCTTAGCAAGTA+AGG + chr5_2:44411969-44411988 None:intergenic 40.0%
TCAGTTAGCTCAGCAAACAA+AGG + chr5_2:44411990-44412009 None:intergenic 40.0%
! TCCTGTTGCTGTTGATGTTA+AGG - chr5_2:44412019-44412038 Msa0771550:CDS 40.0%
ACCTTAACATCAACAGCAAC+AGG + chr5_2:44412023-44412042 None:intergenic 40.0%
TTGGTTGCACCAATTTGACT+AGG - chr5_2:44412237-44412256 Msa0771550:CDS 40.0%
TTGCACCAATTTGACTAGGA+AGG - chr5_2:44412241-44412260 Msa0771550:CDS 40.0%
!! CTAGTTTCCTTTGAAGCTGG+TGG - chr5_2:44410292-44410311 Msa0771550:three_prime_UTR 45.0%
GACATGCATTCCCTACAATC+AGG + chr5_2:44411300-44411319 None:intergenic 45.0%
TGCATGTCCCAAAATCACGA+TGG - chr5_2:44411312-44411331 Msa0771550:intron 45.0%
! TAGCGTGCCATCGTGATTTT+GGG + chr5_2:44411322-44411341 None:intergenic 45.0%
! ATAGCGTGCCATCGTGATTT+TGG + chr5_2:44411323-44411342 None:intergenic 45.0%
! TCGTTTTCGGTCGAGGTTTT+AGG + chr5_2:44411699-44411718 None:intergenic 45.0%
GCGGATCAAGCAAAGTTCTA+TGG + chr5_2:44411755-44411774 None:intergenic 45.0%
CTATAAGTGACTGCTGAGAG+TGG + chr5_2:44411804-44411823 None:intergenic 45.0%
GCTTTGTGAAGGAAACCTTG+TGG - chr5_2:44411914-44411933 Msa0771550:intron 45.0%
AACCTTGTGGTATGTCCAGA+AGG - chr5_2:44411927-44411946 Msa0771550:intron 45.0%
TTCCTTCTGGACATACCACA+AGG + chr5_2:44411932-44411951 None:intergenic 45.0%
! GTAGAGAGCCTTACTTGCTA+AGG - chr5_2:44411958-44411977 Msa0771550:intron 45.0%
CATCTTGCTGCAGGTAAGGT+TGG + chr5_2:44410164-44410183 None:intergenic 50.0%
! TACCTGGTGGGTCAGTAGTG+TGG - chr5_2:44412420-44412439 Msa0771550:CDS 55.0%
GCGCGACCGAGCAAGAGATA+GGG - chr5_2:44411875-44411894 Msa0771550:intron 60.0%
CACCACACTACTGACCCACC+AGG + chr5_2:44412425-44412444 None:intergenic 60.0%
GGTGGGTCAGTAGTGTGGTG+AGG - chr5_2:44412425-44412444 Msa0771550:CDS 60.0%
CGCGCGACCGAGCAAGAGAT+AGG - chr5_2:44411874-44411893 Msa0771550:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 44410096 44412629 44410096 ID=Msa0771550;Name=Msa0771550
chr5_2 mRNA 44410096 44412629 44410096 ID=Msa0771550-mRNA-1;Parent=Msa0771550;Name=Msa0771550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=85|1|1|1|1|1|2|301|489
chr5_2 exon 44410096 44411287 44410096 ID=Msa0771550-mRNA-1:exon:13527;Parent=Msa0771550-mRNA-1
chr5_2 exon 44411966 44412629 44411966 ID=Msa0771550-mRNA-1:exon:13526;Parent=Msa0771550-mRNA-1
chr5_2 five_prime_UTR 44412545 44412629 44412545 ID=Msa0771550-mRNA-1:five_prime_utr;Parent=Msa0771550-mRNA-1
chr5_2 CDS 44411966 44412544 44411966 ID=Msa0771550-mRNA-1:cds;Parent=Msa0771550-mRNA-1
chr5_2 CDS 44410397 44411287 44410397 ID=Msa0771550-mRNA-1:cds;Parent=Msa0771550-mRNA-1
chr5_2 three_prime_UTR 44410096 44410396 44410096 ID=Msa0771550-mRNA-1:three_prime_utr;Parent=Msa0771550-mRNA-1
Gene Sequence

>Msa0771550

ATGAAAAACCTTTCACTTGCCAACAAAACTTCTAAACTTTCAAATAAAACACTAGTTTTTGATTTTGAAGGCACTCTTCTAAGATCCATTTCACTCTTCCCTTACTTCATGCTAGTTTCCTTTGAAGCTGGTGGAATACTAAGATCTATCATTTTGTTTCTTTCATACCCTTTGGTATGGTTAGTTGGTGAGTATCAACTAGGTTTGAAGATTATGGTTTTCTTAACCTTTTTTGGTATAAGAAAAGACACTTTAAGGGTAGGAATGGCTGTTCTTCCAAAGTTCTACTTAGAAGATGTTGGTTGGGAAGGATTTGAAGCTGTGATGTGTTGTGAGAGAAAAGTTGCATCTAGTAAGTTACCAAGAATAATGGTTGAAGGTTTCTTGAAAGACTATTTAGGGGTTGAAGCTATTGTAGCAAGAGACATAAAGTCATACAATGGTTACTTTTTGGGTTTGTTTGAGAAACATAAAACAACCACTTCCTATGATGTTAAAGCAACAAGCAAAGAGAATAGTATTGGTATTATTGGTAGCCATATTGAGTATATTGACCAGAAACTTTTCCCTCATTACAAGAAGATTTGCTTTATGTTAAGTTCTGATGAGAGGAGAAATTGGCGTGCATTACCAAGAAAGTTGTTTCCTAAGCCATTGATTTTCCATGATGGAAGATTGGCTTTCATGCCAACTGTAACATCTTCACTAGTCATGTTCATGTGGTTACCACTTGGACTATTTCTTTCAATTTTCAGAATAACATTTGGAGTTTCATTGCCTTTTAATGCATCAGCTCCAATATTAGCATTTTCAGGGACAAGAAGCACAATTTCAAGACCTAAAACCTCGACCGAAAACGAAAAAAATCAAAAGAATATGCTTTATGTGTGTAACCATAGAACTTTGCTTGATCCGCTTTATATATCACACACTATAAACAAACCACTCTCAGCAGTCACTTATAGCATGAGTAGAGTCAATGAGATTATTTCCCCCATTAAAACAACGCGATTAACGCGCGACCGAGCAAGAGATAGGGATTCAATGGAGAAAATGCTTTGTGAAGGAAACCTTGTGGTATGTCCAGAAGGAACAACATGTAGAGAGCCTTACTTGCTAAGGTTTAGTCCTTTGTTTGCTGAGCTAACTGATGATATTGTTCCTGTTGCTGTTGATGTTAAGGTTAGTATGTTTTATGGAACAACAGCTAGTGGATATAAATGTTTGGATCCTGTTTTTCATTACTTGAATCCAAATCCTGTTTACTTTGTTAAAATACTTGAGAGGATACCAACATCACAAACATGTCAAAAAGGTGGAAAATCTCCTGTTGAAGTTGCTAATTTTGCGCAAAATGAAATTGGAAAGAGTTTGGGATTTGGTTGCACCAATTTGACTAGGAAGGACAAATATATGATTTTGGCAGGTAATGAAGGTGTGAGTAGCAATAACAAACAATCCTTGTCATAA
Protein sequence

>Msa0771550

MKNLSLANKTSKLSNKTLVFDFEGTLLRSISLFPYFMLVSFEAGGILRSIILFLSYPLVWLVGEYQLGLKIMVFLTFFGIRKDTLRVGMAVLPKFYLEDVGWEGFEAVMCCERKVASSKLPRIMVEGFLKDYLGVEAIVARDIKSYNGYFLGLFEKHKTTTSYDVKATSKENSIGIIGSHIEYIDQKLFPHYKKICFMLSSDERRNWRALPRKLFPKPLIFHDGRLAFMPTVTSSLVMFMWLPLGLFLSIFRITFGVSLPFNASAPILAFSGTRSTISRPKTSTENEKNQKNMLYVCNHRTLLDPLYISHTINKPLSAVTYSMSRVNEIISPIKTTRLTRDRARDRDSMEKMLCEGNLVVCPEGTTCREPYLLRFSPLFAELTDDIVPVAVDVKVSMFYGTTASGYKCLDPVFHYLNPNPVYFVKILERIPTSQTCQKGGKSPVEVANFAQNEIGKSLGFGCTNLTRKDKYMILAGNEGVSSNNKQSLS*