AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0731150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731150 MtrunA17_Chr5g0425071 96.933 489 15 0 1 489 1 489 0.0 982
Msa0731150 MtrunA17_Chr1g0194391 51.738 489 225 8 5 488 54 536 3.48e-171 493
Msa0731150 MtrunA17_Chr5g0443341 49.474 475 221 8 15 480 64 528 2.43e-158 460
Msa0731150 MtrunA17_Chr3g0096481 49.167 480 226 9 15 485 59 529 2.24e-155 452
Msa0731150 MtrunA17_Chr8g0348781 42.798 486 256 8 8 479 18 495 3.11e-121 364
Msa0731150 MtrunA17_Chr4g0005991 42.300 487 266 8 10 487 15 495 8.46e-121 362
Msa0731150 MtrunA17_Chr1g0170421 41.010 495 272 11 8 487 45 534 4.78e-120 364
Msa0731150 MtrunA17_Chr5g0435581 40.125 481 263 9 17 482 3 473 2.36e-116 351
Msa0731150 MtrunA17_Chr7g0241831 39.468 489 290 4 2 486 10 496 1.01e-115 350
Msa0731150 MtrunA17_Chr3g0096591 54.945 273 116 3 210 479 1 269 1.39e-108 323
Msa0731150 MtrunA17_Chr3g0096581 40.972 144 83 2 15 157 63 205 4.54e-30 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731150 AT4G01950.1 51.446 484 222 10 11 484 40 520 3.37e-169 487
Msa0731150 AT4G01950.2 51.446 484 222 10 11 484 68 548 8.76e-169 487
Msa0731150 AT1G02390.1 50.000 490 222 8 8 480 43 526 6.80e-165 477
Msa0731150 AT1G06520.1 46.998 483 233 10 17 480 105 583 4.01e-149 439
Msa0731150 AT2G38110.1 42.227 476 260 9 14 479 22 492 5.98e-119 358
Msa0731150 AT3G11430.1 39.959 483 267 9 14 482 9 482 9.92e-119 358
Msa0731150 AT5G06090.1 40.329 486 271 12 9 482 2 480 4.98e-118 356
Msa0731150 AT1G01610.1 42.380 479 263 8 17 485 23 498 3.31e-116 351
Msa0731150 AT4G00400.1 41.949 472 261 7 17 479 23 490 1.08e-110 337
Msa0731150 AT3G11325.1 32.104 461 213 9 26 482 6 370 7.15e-72 233
Msa0731150 AT3G11325.2 32.104 461 213 9 26 482 9 373 8.83e-72 233

Find 89 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAATGAGGGAAAAGTTTC+TGG 0.203098 5_1:+41454585 None:intergenic
GTATAAGAAAAGACACTTTA+AGG 0.207106 5_1:-41454904 Msa0731150:CDS
GAGAATAGTATTGGTATTAT+TGG 0.218296 5_1:-41454629 Msa0731150:CDS
TCAACTAGGTTTGAAGATTA+TGG 0.218722 5_1:-41454945 Msa0731150:CDS
GAAAGACAAATATATGATTT+TGG 0.225097 5_1:-41453057 Msa0731150:CDS
ATTAAAACTAAGATAGAAAT+AGG 0.231887 5_1:+41452934 None:intergenic
AAATGAAATTGGAAAGAGTT+TGG 0.246873 5_1:-41453105 Msa0731150:CDS
AAACCTTCAACCATTATTCT+TGG 0.248292 5_1:+41454779 None:intergenic
AATTGTAGTTATTCTTGTTT+TGG 0.271963 5_1:+41455202 None:intergenic
GTGATGTACACAATATTATA+AGG 0.272620 5_1:-41452710 Msa0731150:three_prime_UTR
TTAACAAAGTAAACAGGATT+TGG 0.272628 5_1:+41453206 None:intergenic
GGTTTCTTGAAAGACTATTT+AGG 0.280227 5_1:-41454761 Msa0731150:CDS
GTCCCTGAAAATGCTAATAT+TGG 0.280666 5_1:+41453662 None:intergenic
ATTGGAGCTGATGCATTAAA+AGG 0.310915 5_1:+41453680 None:intergenic
TCTGGTCAATATACTCAATA+TGG 0.319173 5_1:+41454603 None:intergenic
TTTGTCTTTCCTAGTCAAAT+TGG 0.322666 5_1:+41453070 None:intergenic
AATGAAATTGGAAAGAGTTT+GGG 0.331774 5_1:-41453104 Msa0731150:CDS
TTTGTTTCTTTCATACCCTT+TGG 0.336221 5_1:-41454987 Msa0731150:CDS
ACATCACAAACATGTCAAAA+AGG 0.340009 5_1:-41453164 Msa0731150:CDS
TCTCTACAAGTTGTTCCTTC+TGG 0.351163 5_1:+41453374 None:intergenic
GTTCTGATGAGAGGAGAAAT+TGG 0.353548 5_1:-41453859 Msa0731150:CDS
GTGGATCAAGCAAAGTTCTA+TGG 0.367532 5_1:+41453564 None:intergenic
TACCCTTTGGTATGGTTAGT+TGG 0.374543 5_1:-41454974 Msa0731150:CDS
TCCTGTTGCTGTTGATGTTA+AGG 0.382374 5_1:-41453297 Msa0731150:CDS
TGACACACAAATTAACTTGT+AGG 0.389095 5_1:+41452974 None:intergenic
GCTTGTTGCTTTAACATCAT+AGG 0.399072 5_1:+41454655 None:intergenic
ATTGGAAAGAGTTTGGGATT+TGG 0.401189 5_1:-41453098 Msa0731150:CDS
TATAAGAAAAGACACTTTAA+GGG 0.412950 5_1:-41454903 Msa0731150:CDS
ACAAAATTAGCAACTTCAAC+AGG 0.421254 5_1:+41453131 None:intergenic
TTCTTTCATACCCTTTGGTA+TGG 0.423298 5_1:-41454982 Msa0731150:CDS
TAGTAAGTTACCAAGAATAA+TGG 0.425255 5_1:-41454789 Msa0731150:CDS
ACCTTAACATCAACAGCAAC+AGG 0.427108 5_1:+41453296 None:intergenic
GGACTAAACCTTAGCAAGTA+AGG 0.432275 5_1:+41453350 None:intergenic
CATCTTGCTGCAGGTAAGGT+TGG 0.435384 5_1:+41455159 None:intergenic
AGCTGGTGGATATAAATGTT+TGG 0.437289 5_1:-41453252 Msa0731150:CDS
ATGCTAGTTTCCTTTGAAGC+TGG 0.441903 5_1:-41455031 Msa0731150:CDS
CTACTTAGAAGATGTTGGTT+GGG 0.444813 5_1:-41454855 Msa0731150:CDS
TTACCAAGAATAATGGTTGA+AGG 0.459458 5_1:-41454782 Msa0731150:CDS
TCATGGAAAATCAATGGCTT+AGG 0.460797 5_1:+41453812 None:intergenic
AAGGAAACTAGCATGAAGTA+AGG 0.462736 5_1:+41455040 None:intergenic
TTAGAAGATGTTGGTTGGGA+AGG 0.468636 5_1:-41454851 Msa0731150:CDS
CGCGCGACCGAGCAAGAGAC+AGG 0.475152 5_1:-41453442 Msa0731150:CDS
TGATGTACACAATATTATAA+GGG 0.476420 5_1:-41452709 Msa0731150:three_prime_UTR
ACATCTTCTAAGTAGAACTT+TGG 0.478280 5_1:+41454863 None:intergenic
AGCAAGAGACAGGGATTCAA+TGG 0.480606 5_1:-41453432 Msa0731150:CDS
CTAGTTTCCTTTGAAGCTGG+TGG 0.484232 5_1:-41455028 Msa0731150:CDS
CTTCCATCATGGAAAATCAA+TGG 0.487517 5_1:+41453806 None:intergenic
ATAGTGTGTGATATATAAAG+TGG 0.489764 5_1:+41453545 None:intergenic
TTGAATCCCTGTCTCTTGCT+CGG 0.491801 5_1:+41453435 None:intergenic
TGCTTTAACATCATAGGAAG+TGG 0.493418 5_1:+41454661 None:intergenic
TAGTATTCCACCAGCTTCAA+AGG 0.493486 5_1:+41455021 None:intergenic
TTCCTTCTGGACACACTACA+AGG 0.493940 5_1:+41453387 None:intergenic
ACAAGCAAAGAGAATAGTAT+TGG 0.508669 5_1:-41454638 Msa0731150:CDS
TCTACTTAGAAGATGTTGGT+TGG 0.515294 5_1:-41454856 Msa0731150:CDS
GTGGGTCAGTAGTGTGGTGA+GGG 0.516042 5_1:-41452890 Msa0731150:three_prime_UTR
CACCAACTAACCATACCAAA+GGG 0.528093 5_1:+41454972 None:intergenic
AGACACTTTAAGGGTAGGAA+TGG 0.534443 5_1:-41454894 Msa0731150:CDS
AGAAACCAAACCTTGTAATG+AGG 0.540345 5_1:+41454572 None:intergenic
GTAGAGAGCCTTACTTGCTA+AGG 0.541715 5_1:-41453358 Msa0731150:CDS
TGAAAGCCAATCTTCCATCA+TGG 0.549956 5_1:+41453795 None:intergenic
GAAACCAAACCTTGTAATGA+GGG 0.554140 5_1:+41454573 None:intergenic
AAGTTCTACTTAGAAGATGT+TGG 0.556652 5_1:-41454860 Msa0731150:CDS
ATGGAGAAAATGCTTTGTGA+AGG 0.565042 5_1:-41453413 Msa0731150:CDS
TTGGTTGCACCAATTTGACT+AGG 0.569693 5_1:-41453079 Msa0731150:CDS
TTATGTTAAGTTCTGATGAG+AGG 0.571837 5_1:-41453868 Msa0731150:CDS
GGTGGGTCAGTAGTGTGGTG+AGG 0.575100 5_1:-41452891 Msa0731150:three_prime_UTR
AGAAAAGACACTTTAAGGGT+AGG 0.579822 5_1:-41454899 Msa0731150:CDS
AGGTTGGTTCTTTAAAACAA+AGG 0.587296 5_1:+41455175 None:intergenic
ATGTTCATGTGGTTACCACT+TGG 0.589495 5_1:-41453746 Msa0731150:CDS
AACCTTGTAGTGTGTCCAGA+AGG 0.589664 5_1:-41453389 Msa0731150:CDS
AGGAAACTAGCATGAAGTAA+GGG 0.591307 5_1:+41455041 None:intergenic
AGTAATGAATATTACCTGGT+GGG 0.595121 5_1:-41452908 Msa0731150:three_prime_UTR
TCACCAACTAACCATACCAA+AGG 0.596402 5_1:+41454971 None:intergenic
ATTGAAAGAAATAGTCCAAG+TGG 0.598959 5_1:+41453731 None:intergenic
AACTTGCAGTTATTATGACA+AGG 0.600625 5_1:+41452998 None:intergenic
TTAAAGTAATGAATATTACC+TGG 0.601181 5_1:-41452912 Msa0731150:three_prime_UTR
TTAGTTGGTGAGTATCAACT+AGG 0.603020 5_1:-41454959 Msa0731150:CDS
ACTAGTGAAGATGTTACAGT+TGG 0.610755 5_1:+41453770 None:intergenic
CTTCACTAGTCATGTTCATG+TGG 0.612621 5_1:-41453757 Msa0731150:CDS
ATAGCTATAAGTAAAATCAA+AGG 0.615844 5_1:-41452742 Msa0731150:three_prime_UTR
ACTTTGTTAAAATACTTGAG+AGG 0.622036 5_1:-41453193 Msa0731150:CDS
AAGTAATGAATATTACCTGG+TGG 0.625285 5_1:-41452909 Msa0731150:three_prime_UTR
CACCACACTACTGACCCACC+AGG 0.632024 5_1:+41452894 None:intergenic
GAAGTAAGGGAAGAGTGAAG+TGG 0.637079 5_1:+41455054 None:intergenic
CTATATGTGACTGCTGAGAG+TGG 0.637208 5_1:+41453515 None:intergenic
GCGCGACCGAGCAAGAGACA+GGG 0.649802 5_1:-41453441 Msa0731150:CDS
AGAGACATAAAGTCATACAA+TGG 0.657033 5_1:-41454719 Msa0731150:CDS
TCAGTTAGTTCAGCAAACAA+AGG 0.659246 5_1:+41453329 None:intergenic
TACCTGGTGGGTCAGTAGTG+TGG 0.678698 5_1:-41452896 Msa0731150:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTAGATGTTTATAT+AGG - chr5_1:41455118-41455137 Msa0731150:CDS 10.0%
!!! TGTTTATACTTTAAAAATCA+TGG - chr5_1:41453401-41453420 Msa0731150:CDS 15.0%
!!! AGCTTTTGTTAAAATTATTA+TGG - chr5_1:41453499-41453518 Msa0731150:CDS 15.0%
!!! TTTTAGACAAAAAAAAAAGT+TGG + chr5_1:41453995-41454014 None:intergenic 15.0%
!! ATTAAAACTAAGATAGAAAT+AGG + chr5_1:41455001-41455020 None:intergenic 15.0%
!!! AATTGTAGTTATTCTTGTTT+TGG + chr5_1:41452733-41452752 None:intergenic 20.0%
!! TATAAGAAAAGACACTTTAA+GGG - chr5_1:41453029-41453048 Msa0731150:CDS 20.0%
!!! TCAATTTTCAGAATAACATT+TGG - chr5_1:41454219-41454238 Msa0731150:intron 20.0%
!!! CTTTTGATTTTTTTCGTTTT+CGG + chr5_1:41454328-41454347 None:intergenic 20.0%
!!! AGTATTTTAACAAAGTAAAC+AGG + chr5_1:41454735-41454754 None:intergenic 20.0%
!!! AATTTTGTGCAAAATGAAAT+TGG - chr5_1:41454816-41454835 Msa0731150:CDS 20.0%
!!! GAAAGACAAATATATGATTT+TGG - chr5_1:41454875-41454894 Msa0731150:CDS 20.0%
!! TTAAAGTAATGAATATTACC+TGG - chr5_1:41455020-41455039 Msa0731150:CDS 20.0%
!! ATAGCTATAAGTAAAATCAA+AGG - chr5_1:41455190-41455209 Msa0731150:five_prime_UTR 20.0%
!! TGATGTACACAATATTATAA+GGG - chr5_1:41455223-41455242 Msa0731150:five_prime_UTR 20.0%
!!! TGAAAGTTTAGAAGTTTTGT+TGG + chr5_1:41452815-41452834 None:intergenic 25.0%
!!! ACACTAGTTTTTGATTTTGA+AGG - chr5_1:41452841-41452860 Msa0731150:three_prime_UTR 25.0%
!! ATGGTTTTCTTAACCTTTTT+TGG - chr5_1:41453006-41453025 Msa0731150:exon 25.0%
!! GTCTTTTCTTATACCAAAAA+AGG + chr5_1:41453022-41453041 None:intergenic 25.0%
! GTATAAGAAAAGACACTTTA+AGG - chr5_1:41453028-41453047 Msa0731150:CDS 25.0%
! TAGTAAGTTACCAAGAATAA+TGG - chr5_1:41453143-41453162 Msa0731150:CDS 25.0%
!!! TCATACAATGGTTACTTTTT+GGG - chr5_1:41453225-41453244 Msa0731150:CDS 25.0%
!!! GAGAATAGTATTGGTATTAT+TGG - chr5_1:41453303-41453322 Msa0731150:CDS 25.0%
! AGCTTCAAAAAAAAAAACGA+GGG + chr5_1:41453470-41453489 None:intergenic 25.0%
! TTTCAAAGAAAACGAAGTAT+TGG + chr5_1:41453781-41453800 None:intergenic 25.0%
! ATAGTGTGTGATATATAAAG+TGG + chr5_1:41454390-41454409 None:intergenic 25.0%
!!! TTAATCGCGTTGTTTTAATA+GGG + chr5_1:41454470-41454489 None:intergenic 25.0%
!! GTTAAGGTTAGTATGTTTTA+TGG - chr5_1:41454651-41454670 Msa0731150:CDS 25.0%
! TTAACAAAGTAAACAGGATT+TGG + chr5_1:41454729-41454748 None:intergenic 25.0%
! ACTTTGTTAAAATACTTGAG+AGG - chr5_1:41454739-41454758 Msa0731150:CDS 25.0%
! AAATGAAATTGGAAAGAGTT+TGG - chr5_1:41454827-41454846 Msa0731150:CDS 25.0%
! AATGAAATTGGAAAGAGTTT+GGG - chr5_1:41454828-41454847 Msa0731150:CDS 25.0%
! GTGATGTACACAATATTATA+AGG - chr5_1:41455222-41455241 Msa0731150:five_prime_UTR 25.0%
!! AGGTTGGTTCTTTAAAACAA+AGG + chr5_1:41452760-41452779 None:intergenic 30.0%
! TTTGTTTCTTTCATACCCTT+TGG - chr5_1:41452945-41452964 Msa0731150:three_prime_UTR 30.0%
TCAACTAGGTTTGAAGATTA+TGG - chr5_1:41452987-41453006 Msa0731150:three_prime_UTR 30.0%
ACATCTTCTAAGTAGAACTT+TGG + chr5_1:41453072-41453091 None:intergenic 30.0%
AAGTTCTACTTAGAAGATGT+TGG - chr5_1:41453072-41453091 Msa0731150:CDS 30.0%
TTACCAAGAATAATGGTTGA+AGG - chr5_1:41453150-41453169 Msa0731150:CDS 30.0%
AAACCTTCAACCATTATTCT+TGG + chr5_1:41453156-41453175 None:intergenic 30.0%
GGTTTCTTGAAAGACTATTT+AGG - chr5_1:41453171-41453190 Msa0731150:CDS 30.0%
AGAGACATAAAGTCATACAA+TGG - chr5_1:41453213-41453232 Msa0731150:CDS 30.0%
!!! GTCATACAATGGTTACTTTT+TGG - chr5_1:41453224-41453243 Msa0731150:CDS 30.0%
ACAAGCAAAGAGAATAGTAT+TGG - chr5_1:41453294-41453313 Msa0731150:CDS 30.0%
TCTGGTCAATATACTCAATA+TGG + chr5_1:41453332-41453351 None:intergenic 30.0%
!!! ACAAGGTTTGGTTTCTTTTT+TGG - chr5_1:41453367-41453386 Msa0731150:CDS 30.0%
GAGCTTCAAAAAAAAAAACG+AGG + chr5_1:41453471-41453490 None:intergenic 30.0%
TGTTCATAGTCGCTAATTAA+CGG + chr5_1:41453533-41453552 None:intergenic 30.0%
TGCATTATTGTCTAATTTCG+CGG - chr5_1:41453610-41453629 Msa0731150:CDS 30.0%
AATTTCGCGGTTTAATACTA+TGG - chr5_1:41453623-41453642 Msa0731150:CDS 30.0%
! TTATGTTAAGTTCTGATGAG+AGG - chr5_1:41454064-41454083 Msa0731150:intron 30.0%
ATTGAAAGAAATAGTCCAAG+TGG + chr5_1:41454204-41454223 None:intergenic 30.0%
! CTCCAATATTAGCATTTTCA+GGG - chr5_1:41454268-41454287 Msa0731150:intron 30.0%
!!! TAATCGCGTTGTTTTAATAG+GGG + chr5_1:41454469-41454488 None:intergenic 30.0%
!!! GTTAATCGCGTTGTTTTAAT+AGG + chr5_1:41454471-41454490 None:intergenic 30.0%
GGATTCAAGTAATGAAAAAC+AGG + chr5_1:41454708-41454727 None:intergenic 30.0%
! TTTTGACATGTTTGTGATGT+TGG + chr5_1:41454768-41454787 None:intergenic 30.0%
ACATCACAAACATGTCAAAA+AGG - chr5_1:41454768-41454787 Msa0731150:CDS 30.0%
ACAAAATTAGCAACTTCAAC+AGG + chr5_1:41454804-41454823 None:intergenic 30.0%
TTTGTCTTTCCTAGTCAAAT+TGG + chr5_1:41454865-41454884 None:intergenic 30.0%
!! GACAAATATATGATTTTGGC+AGG - chr5_1:41454879-41454898 Msa0731150:CDS 30.0%
AACTTGCAGTTATTATGACA+AGG + chr5_1:41454937-41454956 None:intergenic 30.0%
!! TGACACACAAATTAACTTGT+AGG + chr5_1:41454961-41454980 None:intergenic 30.0%
AAGTAATGAATATTACCTGG+TGG - chr5_1:41455023-41455042 Msa0731150:CDS 30.0%
AGTAATGAATATTACCTGGT+GGG - chr5_1:41455024-41455043 Msa0731150:CDS 30.0%
!!! AAGTTTTGTTGGCAAGTGAA+AGG + chr5_1:41452804-41452823 None:intergenic 35.0%
AGGAAACTAGCATGAAGTAA+GGG + chr5_1:41452894-41452913 None:intergenic 35.0%
AAGGAAACTAGCATGAAGTA+AGG + chr5_1:41452895-41452914 None:intergenic 35.0%
TTCTTTCATACCCTTTGGTA+TGG - chr5_1:41452950-41452969 Msa0731150:three_prime_UTR 35.0%
!! TTAGTTGGTGAGTATCAACT+AGG - chr5_1:41452973-41452992 Msa0731150:three_prime_UTR 35.0%
! AGAAAAGACACTTTAAGGGT+AGG - chr5_1:41453033-41453052 Msa0731150:CDS 35.0%
TCTACTTAGAAGATGTTGGT+TGG - chr5_1:41453076-41453095 Msa0731150:CDS 35.0%
! CTACTTAGAAGATGTTGGTT+GGG - chr5_1:41453077-41453096 Msa0731150:CDS 35.0%
TGCTTTAACATCATAGGAAG+TGG + chr5_1:41453274-41453293 None:intergenic 35.0%
!! GCTTGTTGCTTTAACATCAT+AGG + chr5_1:41453280-41453299 None:intergenic 35.0%
TGTAATGAGGGAAAAGTTTC+TGG + chr5_1:41453350-41453369 None:intergenic 35.0%
! GAAACTTTTCCCTCATTACA+AGG - chr5_1:41453350-41453369 Msa0731150:CDS 35.0%
! TTTTCCCTCATTACAAGGTT+TGG - chr5_1:41453355-41453374 Msa0731150:CDS 35.0%
GAAACCAAACCTTGTAATGA+GGG + chr5_1:41453362-41453381 None:intergenic 35.0%
AGAAACCAAACCTTGTAATG+AGG + chr5_1:41453363-41453382 None:intergenic 35.0%
GAATGATTGTCCCAAAATCA+TGG - chr5_1:41453925-41453944 Msa0731150:intron 35.0%
!!! GGCTTAGGAAACAACTTTTT+TGG + chr5_1:41454108-41454127 None:intergenic 35.0%
TCATGGAAAATCAATGGCTT+AGG + chr5_1:41454123-41454142 None:intergenic 35.0%
!!! AAGCCATTGATTTTCCATGA+TGG - chr5_1:41454123-41454142 Msa0731150:intron 35.0%
CTTCCATCATGGAAAATCAA+TGG + chr5_1:41454129-41454148 None:intergenic 35.0%
!! GATTTTCCATGATGGAAGAT+TGG - chr5_1:41454131-41454150 Msa0731150:intron 35.0%
ACTAGTGAAGATGTTACAGT+TGG + chr5_1:41454165-41454184 None:intergenic 35.0%
ATTGGAGCTGATGCATTAAA+AGG + chr5_1:41454255-41454274 None:intergenic 35.0%
! GCTCCAATATTAGCATTTTC+AGG - chr5_1:41454267-41454286 Msa0731150:intron 35.0%
GTCCCTGAAAATGCTAATAT+TGG + chr5_1:41454273-41454292 None:intergenic 35.0%
!!! ATTTTTTTCGTTTTCGGTCG+AGG + chr5_1:41454322-41454341 None:intergenic 35.0%
ATGGAGAAAATGCTTTGTGA+AGG - chr5_1:41454519-41454538 Msa0731150:intron 35.0%
TCAGTTAGTTCAGCAAACAA+AGG + chr5_1:41454606-41454625 None:intergenic 35.0%
! ATGTTTTATGGAACAACAGC+TGG - chr5_1:41454663-41454682 Msa0731150:CDS 35.0%
! AGCTGGTGGATATAAATGTT+TGG - chr5_1:41454680-41454699 Msa0731150:CDS 35.0%
TCACAAACATGTCAAAAAGG+TGG - chr5_1:41454771-41454790 Msa0731150:CDS 35.0%
! ATTGGAAAGAGTTTGGGATT+TGG - chr5_1:41454834-41454853 Msa0731150:CDS 35.0%
!! ATGATTTTGGCAGGTAATGA+AGG - chr5_1:41454888-41454907 Msa0731150:CDS 35.0%
! TTTTCATCTTGCTGCAGGTA+AGG + chr5_1:41452780-41452799 None:intergenic 40.0%
! AAGGTTTTTCATCTTGCTGC+AGG + chr5_1:41452785-41452804 None:intergenic 40.0%
!! ATGCTAGTTTCCTTTGAAGC+TGG - chr5_1:41452901-41452920 Msa0731150:three_prime_UTR 40.0%
TAGTATTCCACCAGCTTCAA+AGG + chr5_1:41452914-41452933 None:intergenic 40.0%
TACCCTTTGGTATGGTTAGT+TGG - chr5_1:41452958-41452977 Msa0731150:three_prime_UTR 40.0%
CACCAACTAACCATACCAAA+GGG + chr5_1:41452963-41452982 None:intergenic 40.0%
TCACCAACTAACCATACCAA+AGG + chr5_1:41452964-41452983 None:intergenic 40.0%
! AGACACTTTAAGGGTAGGAA+TGG - chr5_1:41453038-41453057 Msa0731150:CDS 40.0%
! TTAGAAGATGTTGGTTGGGA+AGG - chr5_1:41453081-41453100 Msa0731150:CDS 40.0%
CATAGTCGCTAATTAACGGA+AGG + chr5_1:41453529-41453548 None:intergenic 40.0%
ATACTGCGATTCCTGATTGT+AGG - chr5_1:41453902-41453921 Msa0731150:intron 40.0%
TACTGCGATTCCTGATTGTA+GGG - chr5_1:41453903-41453922 Msa0731150:intron 40.0%
GACAATCATTCCCTACAATC+AGG + chr5_1:41453916-41453935 None:intergenic 40.0%
TGATTGTCCCAAAATCATGG+TGG - chr5_1:41453928-41453947 Msa0731150:intron 40.0%
GTTCTGATGAGAGGAGAAAT+TGG - chr5_1:41454073-41454092 Msa0731150:intron 40.0%
TGAAAGCCAATCTTCCATCA+TGG + chr5_1:41454140-41454159 None:intergenic 40.0%
CTTCACTAGTCATGTTCATG+TGG - chr5_1:41454175-41454194 Msa0731150:intron 40.0%
ATGTTCATGTGGTTACCACT+TGG - chr5_1:41454186-41454205 Msa0731150:intron 40.0%
GTGGATCAAGCAAAGTTCTA+TGG + chr5_1:41454371-41454390 None:intergenic 40.0%
TCTCTACAAGTTGTTCCTTC+TGG + chr5_1:41454561-41454580 None:intergenic 40.0%
GGACTAAACCTTAGCAAGTA+AGG + chr5_1:41454585-41454604 None:intergenic 40.0%
! TCCTGTTGCTGTTGATGTTA+AGG - chr5_1:41454635-41454654 Msa0731150:CDS 40.0%
ACCTTAACATCAACAGCAAC+AGG + chr5_1:41454639-41454658 None:intergenic 40.0%
! TTTTATGGAACAACAGCTGG+TGG - chr5_1:41454666-41454685 Msa0731150:CDS 40.0%
TTGGTTGCACCAATTTGACT+AGG - chr5_1:41454853-41454872 Msa0731150:CDS 40.0%
GAAGTAAGGGAAGAGTGAAG+TGG + chr5_1:41452881-41452900 None:intergenic 45.0%
!! CTAGTTTCCTTTGAAGCTGG+TGG - chr5_1:41452904-41452923 Msa0731150:three_prime_UTR 45.0%
! CGTTTTCGGTCGAGGTTTTA+GGG + chr5_1:41454314-41454333 None:intergenic 45.0%
! TCGTTTTCGGTCGAGGTTTT+AGG + chr5_1:41454315-41454334 None:intergenic 45.0%
CTATATGTGACTGCTGAGAG+TGG + chr5_1:41454420-41454439 None:intergenic 45.0%
TTGAATCCCTGTCTCTTGCT+CGG + chr5_1:41454500-41454519 None:intergenic 45.0%
AGCAAGAGACAGGGATTCAA+TGG - chr5_1:41454500-41454519 Msa0731150:intron 45.0%
AACCTTGTAGTGTGTCCAGA+AGG - chr5_1:41454543-41454562 Msa0731150:intron 45.0%
TTCCTTCTGGACACACTACA+AGG + chr5_1:41454548-41454567 None:intergenic 45.0%
! GTAGAGAGCCTTACTTGCTA+AGG - chr5_1:41454574-41454593 Msa0731150:intron 45.0%
CATCTTGCTGCAGGTAAGGT+TGG + chr5_1:41452776-41452795 None:intergenic 50.0%
! CAGCGTGCCACCATGATTTT+GGG + chr5_1:41453938-41453957 None:intergenic 50.0%
! ACAGCGTGCCACCATGATTT+TGG + chr5_1:41453939-41453958 None:intergenic 50.0%
! TACCTGGTGGGTCAGTAGTG+TGG - chr5_1:41455036-41455055 Msa0731150:CDS 55.0%
GTGGGTCAGTAGTGTGGTGA+GGG - chr5_1:41455042-41455061 Msa0731150:CDS 55.0%
CACCACACTACTGACCCACC+AGG + chr5_1:41455041-41455060 None:intergenic 60.0%
GGTGGGTCAGTAGTGTGGTG+AGG - chr5_1:41455041-41455060 Msa0731150:CDS 60.0%
GCGCGACCGAGCAAGAGACA+GGG - chr5_1:41454491-41454510 Msa0731150:intron 65.0%
CGCGCGACCGAGCAAGAGAC+AGG - chr5_1:41454490-41454509 Msa0731150:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 41452708 41455246 41452708 ID=Msa0731150;Name=Msa0731150
chr5_1 mRNA 41452708 41455246 41452708 ID=Msa0731150-mRNA-1;Parent=Msa0731150;Name=Msa0731150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=85|1|1|1|1|1|2|302|489
chr5_1 exon 41452708 41453900 41452708 ID=Msa0731150-mRNA-1:exon:12047;Parent=Msa0731150-mRNA-1
chr5_1 exon 41454583 41455246 41454583 ID=Msa0731150-mRNA-1:exon:12046;Parent=Msa0731150-mRNA-1
chr5_1 five_prime_UTR 41455162 41455246 41455162 ID=Msa0731150-mRNA-1:five_prime_utr;Parent=Msa0731150-mRNA-1
chr5_1 CDS 41454583 41455161 41454583 ID=Msa0731150-mRNA-1:cds;Parent=Msa0731150-mRNA-1
chr5_1 CDS 41453010 41453900 41453010 ID=Msa0731150-mRNA-1:cds;Parent=Msa0731150-mRNA-1
chr5_1 three_prime_UTR 41452708 41453009 41452708 ID=Msa0731150-mRNA-1:three_prime_utr;Parent=Msa0731150-mRNA-1
Gene Sequence

>Msa0731150

ATGAAAAACCTTTCACTTGCCAACAAAACTTCTAAACTTTCAAATAAAACACTAGTTTTTGATTTTGAAGGCACTCTTCTAAGATCCACTTCACTCTTCCCTTACTTCATGCTAGTTTCCTTTGAAGCTGGTGGAATACTAAGATCTATCATTTTGTTTCTTTCATACCCTTTGGTATGGTTAGTTGGTGAGTATCAACTAGGTTTGAAGATTATGGTTTTCTTAACCTTTTTTGGTATAAGAAAAGACACTTTAAGGGTAGGAATGGCTGTTCTTCCAAAGTTCTACTTAGAAGATGTTGGTTGGGAAGGATTTGAAGCTGTGATGTGTTGTGAGAGAAAAGTTGCATCTAGTAAGTTACCAAGAATAATGGTTGAAGGTTTCTTGAAAGACTATTTAGGTGTTGAAGCTATTGTAGCAAGAGACATAAAGTCATACAATGGTTACTTTTTGGGCTTGTTTGAGAAACATAAAACAACCACTTCCTATGATGTTAAAGCAACAAGCAAAGAGAATAGTATTGGTATTATTGGTAGCCATATTGAGTATATTGACCAGAAACTTTTCCCTCATTACAAGAAGATTTGCTTTATGTTAAGTTCTGATGAGAGGAGAAATTGGCGTGTATTACCAAAAAAGTTGTTTCCTAAGCCATTGATTTTCCATGATGGAAGATTGGCTTTCATGCCAACTGTAACATCTTCACTAGTCATGTTCATGTGGTTACCACTTGGACTATTTCTTTCAATTTTCAGAATAACATTTGGAGTTTCATTACCTTTTAATGCATCAGCTCCAATATTAGCATTTTCAGGGACAAGAAGCACAATTTCAAGCCCTAAAACCTCGACCGAAAACGAAAAAAATCAAAAGAATATGCTTTATGTGTGTAACCATAGAACTTTGCTTGATCCACTTTATATATCACACACTATAAACAAACCACTCTCAGCAGTCACATATAGCATGAGCAGAGTCAATGAGATTATTTCCCCTATTAAAACAACGCGATTAACGCGCGACCGAGCAAGAGACAGGGATTCAATGGAGAAAATGCTTTGTGAAGGAAACCTTGTAGTGTGTCCAGAAGGAACAACTTGTAGAGAGCCTTACTTGCTAAGGTTTAGTCCTTTGTTTGCTGAACTAACTGATGATATTGTTCCTGTTGCTGTTGATGTTAAGGTTAGTATGTTTTATGGAACAACAGCTGGTGGATATAAATGTTTGGATCCTGTTTTTCATTACTTGAATCCAAATCCTGTTTACTTTGTTAAAATACTTGAGAGGATACCAACATCACAAACATGTCAAAAAGGTGGAAAATCTCCTGTTGAAGTTGCTAATTTTGTGCAAAATGAAATTGGAAAGAGTTTGGGATTTGGTTGCACCAATTTGACTAGGAAAGACAAATATATGATTTTGGCAGGTAATGAAGGTGTGAGTAGCAATAACAAACAATCCTTGTCATAA
Protein sequence

>Msa0731150

MKNLSLANKTSKLSNKTLVFDFEGTLLRSTSLFPYFMLVSFEAGGILRSIILFLSYPLVWLVGEYQLGLKIMVFLTFFGIRKDTLRVGMAVLPKFYLEDVGWEGFEAVMCCERKVASSKLPRIMVEGFLKDYLGVEAIVARDIKSYNGYFLGLFEKHKTTTSYDVKATSKENSIGIIGSHIEYIDQKLFPHYKKICFMLSSDERRNWRVLPKKLFPKPLIFHDGRLAFMPTVTSSLVMFMWLPLGLFLSIFRITFGVSLPFNASAPILAFSGTRSTISSPKTSTENEKNQKNMLYVCNHRTLLDPLYISHTINKPLSAVTYSMSRVNEIISPIKTTRLTRDRARDRDSMEKMLCEGNLVVCPEGTTCREPYLLRFSPLFAELTDDIVPVAVDVKVSMFYGTTAGGYKCLDPVFHYLNPNPVYFVKILERIPTSQTCQKGGKSPVEVANFVQNEIGKSLGFGCTNLTRKDKYMILAGNEGVSSNNKQSLS*