AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0850030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850030 MtrunA17_Chr5g0425071 96.933 489 15 0 1 489 1 489 0.0 981
Msa0850030 MtrunA17_Chr1g0194391 51.943 489 224 8 5 488 54 536 2.92e-171 493
Msa0850030 MtrunA17_Chr5g0443341 49.474 475 221 8 15 480 64 528 3.13e-158 460
Msa0850030 MtrunA17_Chr3g0096481 49.167 480 226 9 15 485 59 529 5.23e-155 451
Msa0850030 MtrunA17_Chr8g0348781 42.593 486 257 8 8 479 18 495 1.01e-121 365
Msa0850030 MtrunA17_Chr4g0005991 42.300 487 266 8 10 487 15 495 4.52e-121 363
Msa0850030 MtrunA17_Chr1g0170421 41.010 495 272 11 8 487 45 534 1.70e-120 365
Msa0850030 MtrunA17_Chr5g0435581 40.125 481 263 9 17 482 3 473 1.26e-116 352
Msa0850030 MtrunA17_Chr7g0241831 39.200 500 276 7 2 486 10 496 1.59e-116 352
Msa0850030 MtrunA17_Chr3g0096591 55.311 273 115 3 210 479 1 269 2.79e-109 325
Msa0850030 MtrunA17_Chr3g0096581 40.278 144 84 2 15 157 63 205 2.72e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0850030 AT4G01950.1 51.653 484 221 10 11 484 40 520 2.46e-169 488
Msa0850030 AT4G01950.2 51.653 484 221 10 11 484 68 548 9.77e-169 487
Msa0850030 AT1G02390.1 50.204 490 221 8 8 480 43 526 4.49e-165 477
Msa0850030 AT1G06520.1 47.205 483 232 10 17 480 105 583 1.02e-149 440
Msa0850030 AT2G38110.1 42.227 476 260 9 14 479 22 492 2.16e-119 359
Msa0850030 AT3G11430.1 39.959 483 267 9 14 482 9 482 2.96e-119 359
Msa0850030 AT5G06090.1 40.329 486 271 12 9 482 2 480 2.01e-118 357
Msa0850030 AT1G01610.1 42.380 479 263 8 17 485 23 498 8.86e-117 353
Msa0850030 AT4G00400.1 41.949 472 261 7 17 479 23 490 5.65e-111 338
Msa0850030 AT3G11325.2 32.321 461 212 10 26 482 9 373 3.32e-72 234
Msa0850030 AT3G11325.1 32.321 461 212 10 26 482 6 370 3.62e-72 234

Find 93 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCTTGAAAGACTATTT+AGG 0.195935 5_4:-38389795 Msa0850030:CDS
TGTAATGAGGGAAAAGTTTC+TGG 0.203098 5_4:+38389619 None:intergenic
GTATAAGAAAAGACACTTTA+AGG 0.207106 5_4:-38389938 Msa0850030:CDS
GAGAATAGTATTGGTATTAT+TGG 0.218296 5_4:-38389663 Msa0850030:CDS
TCAACTAGGTTTGAAGATTA+TGG 0.218722 5_4:-38389979 Msa0850030:CDS
GAAAGACAAATATATGATTT+TGG 0.225097 5_4:-38388089 Msa0850030:CDS
ATTAAAACTAAGATAGAAAT+AGG 0.231887 5_4:+38387966 None:intergenic
GTCATACAATGGTTACCTTT+TGG 0.239819 5_4:-38389742 Msa0850030:CDS
AAATGAAATTGGAAAGAGTT+TGG 0.246873 5_4:-38388137 Msa0850030:CDS
AAACCTTCAACCATTATTCT+TGG 0.248292 5_4:+38389813 None:intergenic
AATTGTAGTTATACTTGTTT+TGG 0.249787 5_4:+38390236 None:intergenic
GTGATGTACACAATATTATA+AGG 0.272620 5_4:-38387744 Msa0850030:three_prime_UTR
TTAACAAAGTAAACAGGATT+TGG 0.272628 5_4:+38388238 None:intergenic
GTCCCTGAAAATGCTAATAT+TGG 0.280666 5_4:+38388694 None:intergenic
ATTGGAGCTGATGCATTAAA+AGG 0.310915 5_4:+38388712 None:intergenic
TCTGGTCAATATACTCAATA+TGG 0.319173 5_4:+38389637 None:intergenic
TTTGTCTTTCCTAGTCAAAT+TGG 0.322666 5_4:+38388102 None:intergenic
AATGAAATTGGAAAGAGTTT+GGG 0.331774 5_4:-38388136 Msa0850030:CDS
TTTGTTTCTTTCATACCCTT+TGG 0.336221 5_4:-38390021 Msa0850030:CDS
ACATCACAAACATGTCAAAA+AGG 0.340009 5_4:-38388196 Msa0850030:CDS
TCTCTACAAGTTGTTCCTTC+TGG 0.351163 5_4:+38388406 None:intergenic
GTTCTGATGAGAGGAGAAAT+TGG 0.353548 5_4:-38388891 Msa0850030:CDS
GTGGATCAAGCAAAGTTCTA+TGG 0.367532 5_4:+38388596 None:intergenic
GTTTCTTGAAAGACTATTTA+GGG 0.373176 5_4:-38389794 Msa0850030:CDS
TACCCTTTGGTATGGTTAGT+TGG 0.374543 5_4:-38390008 Msa0850030:CDS
TCCTGTTGCTGTTGATGTTA+AGG 0.382374 5_4:-38388329 Msa0850030:CDS
TGACACACAAATTAACTTGT+AGG 0.389095 5_4:+38388006 None:intergenic
GCTTGTTGCTTTAACATCAT+AGG 0.399072 5_4:+38389689 None:intergenic
ATTGGAAAGAGTTTGGGATT+TGG 0.401189 5_4:-38388130 Msa0850030:CDS
TATAAGAAAAGACACTTTAA+GGG 0.412950 5_4:-38389937 Msa0850030:CDS
ACAAAATTAGCAACTTCAAC+AGG 0.421254 5_4:+38388163 None:intergenic
TTCTTTCATACCCTTTGGTA+TGG 0.423298 5_4:-38390016 Msa0850030:CDS
TAGTAAGTTACCAAGAATAA+TGG 0.425255 5_4:-38389823 Msa0850030:CDS
ACCTTAACATCAACAGCAAC+AGG 0.427108 5_4:+38388328 None:intergenic
GGACTAAACCTTAGCAAGTA+AGG 0.432275 5_4:+38388382 None:intergenic
CATCTTGCTGCAGGTAAGGT+TGG 0.435384 5_4:+38390193 None:intergenic
ATGCTAGTTTCCTTTGAAGC+TGG 0.441903 5_4:-38390065 Msa0850030:CDS
CTACTTAGAAGATGTTGGTT+GGG 0.444813 5_4:-38389889 Msa0850030:CDS
AGCTAGTGGATATAAATGTT+TGG 0.447892 5_4:-38388284 Msa0850030:CDS
TTACCAAGAATAATGGTTGA+AGG 0.459458 5_4:-38389816 Msa0850030:CDS
TCATGGAAAATCAATGGCTT+AGG 0.460797 5_4:+38388844 None:intergenic
AAGGAAACTAGCATGAAGTA+AGG 0.462736 5_4:+38390074 None:intergenic
TTAGAAGATGTTGGTTGGGA+AGG 0.468636 5_4:-38389885 Msa0850030:CDS
CGCGCGACCGAGCAAGAGAC+AGG 0.475152 5_4:-38388474 Msa0850030:CDS
TGATGTACACAATATTATAA+GGG 0.476420 5_4:-38387743 Msa0850030:three_prime_UTR
ACATCTTCTAAGTAGAACTT+TGG 0.478280 5_4:+38389897 None:intergenic
AGCAAGAGACAGGGATTCAA+TGG 0.480606 5_4:-38388464 Msa0850030:CDS
CTAGTTTCCTTTGAAGCTGG+TGG 0.484232 5_4:-38390062 Msa0850030:CDS
CTTCCATCATGGAAAATCAA+TGG 0.487517 5_4:+38388838 None:intergenic
ATAGTGTGTGATATATAAAG+TGG 0.489764 5_4:+38388577 None:intergenic
TTGAATCCCTGTCTCTTGCT+CGG 0.491801 5_4:+38388467 None:intergenic
TGCTTTAACATCATAGGAAG+TGG 0.493418 5_4:+38389695 None:intergenic
TAGTATTCCACCAGCTTCAA+AGG 0.493486 5_4:+38390055 None:intergenic
TTCCTTCTGGACACACTACA+AGG 0.493940 5_4:+38388419 None:intergenic
GTTTCTCAAACAAACCCAAA+AGG 0.507148 5_4:+38389727 None:intergenic
ACAAGCAAAGAGAATAGTAT+TGG 0.508669 5_4:-38389672 Msa0850030:CDS
TCTACTTAGAAGATGTTGGT+TGG 0.515294 5_4:-38389890 Msa0850030:CDS
GTGGGTCAGTAGTGTGGTGA+GGG 0.516042 5_4:-38387922 Msa0850030:three_prime_UTR
CACCAACTAACCATACCAAA+GGG 0.528093 5_4:+38390006 None:intergenic
AGACACTTTAAGGGTAGGAA+TGG 0.534443 5_4:-38389928 Msa0850030:CDS
AGAAACCAAACCTTGTAATG+AGG 0.540345 5_4:+38389606 None:intergenic
GTAGAGAGCCTTACTTGCTA+AGG 0.541715 5_4:-38388390 Msa0850030:CDS
TGAAAGCCAATCTTCCATCA+TGG 0.549956 5_4:+38388827 None:intergenic
GAAACCAAACCTTGTAATGA+GGG 0.554140 5_4:+38389607 None:intergenic
AAGTTCTACTTAGAAGATGT+TGG 0.556652 5_4:-38389894 Msa0850030:CDS
ATGGAGAAAATGCTTTGTGA+AGG 0.565042 5_4:-38388445 Msa0850030:CDS
TTGGTTGCACCAATTTGACT+AGG 0.569693 5_4:-38388111 Msa0850030:CDS
TTATGTTAAGTTCTGATGAG+AGG 0.571837 5_4:-38388900 Msa0850030:CDS
GGTGGGTCAGTAGTGTGGTG+AGG 0.575100 5_4:-38387923 Msa0850030:three_prime_UTR
AGAAAAGACACTTTAAGGGT+AGG 0.579822 5_4:-38389933 Msa0850030:CDS
AGGTTGGTTCTTTAAAACAA+AGG 0.587296 5_4:+38390209 None:intergenic
ATGTTCATGTGGTTACCACT+TGG 0.589495 5_4:-38388778 Msa0850030:CDS
AACCTTGTAGTGTGTCCAGA+AGG 0.589664 5_4:-38388421 Msa0850030:CDS
AGGAAACTAGCATGAAGTAA+GGG 0.591307 5_4:+38390075 None:intergenic
AGTAATGAATATTACCTGGT+GGG 0.595121 5_4:-38387940 Msa0850030:three_prime_UTR
TCACCAACTAACCATACCAA+AGG 0.596402 5_4:+38390005 None:intergenic
ATTGAAAGAAATAGTCCAAG+TGG 0.598959 5_4:+38388763 None:intergenic
AACTTGCAGTTATTATGACA+AGG 0.600625 5_4:+38388030 None:intergenic
TTAAAGTAATGAATATTACC+TGG 0.601181 5_4:-38387944 Msa0850030:three_prime_UTR
TTAGTTGGTGAGTATCAACT+AGG 0.603020 5_4:-38389993 Msa0850030:CDS
ACTAGTGAAGATGTTACAGT+TGG 0.610755 5_4:+38388802 None:intergenic
CTTCACTAGTCATGTTCATG+TGG 0.612621 5_4:-38388789 Msa0850030:CDS
ATAGCTATAAGTAAAATCAA+AGG 0.615844 5_4:-38387776 Msa0850030:three_prime_UTR
ACTTTGTTAAAATACTTGAG+AGG 0.622036 5_4:-38388225 Msa0850030:CDS
AAGTAATGAATATTACCTGG+TGG 0.625285 5_4:-38387941 Msa0850030:three_prime_UTR
CACCACACTACTGACCCACC+AGG 0.632024 5_4:+38387926 None:intergenic
GAAGTAAGGGAAGAGTGAAG+TGG 0.637079 5_4:+38390088 None:intergenic
CTATATGTGACTGCTGAGAG+TGG 0.637208 5_4:+38388547 None:intergenic
TTTCTTGAAAGACTATTTAG+GGG 0.640361 5_4:-38389793 Msa0850030:CDS
GCGCGACCGAGCAAGAGACA+GGG 0.649802 5_4:-38388473 Msa0850030:CDS
AGAGACATAAAGTCATACAA+TGG 0.657033 5_4:-38389753 Msa0850030:CDS
TCAGTTAGTTCAGCAAACAA+AGG 0.659246 5_4:+38388361 None:intergenic
TACCTGGTGGGTCAGTAGTG+TGG 0.678698 5_4:-38387928 Msa0850030:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAAAAATTGTAATAATTT+TGG - chr5_4:38390139-38390158 Msa0850030:CDS 10.0%
!!! AGCTTTTGTTAAAATTATTA+TGG - chr5_4:38388532-38388551 Msa0850030:CDS 15.0%
!!! TTTTAGACAAAAAAAAAAGT+TGG + chr5_4:38389031-38389050 None:intergenic 15.0%
!! ATTAAAACTAAGATAGAAAT+AGG + chr5_4:38390037-38390056 None:intergenic 15.0%
!!! ATAATTTTGGATGTTTATAT+AGG - chr5_4:38390152-38390171 Msa0850030:CDS 15.0%
!!! AATTGTAGTTATACTTGTTT+TGG + chr5_4:38387767-38387786 None:intergenic 20.0%
!! TATAAGAAAAGACACTTTAA+GGG - chr5_4:38388063-38388082 Msa0850030:CDS 20.0%
!!! TCAATTTTCAGAATAACATT+TGG - chr5_4:38389255-38389274 Msa0850030:intron 20.0%
!!! CTTTTGATTTTTTTCGTTTT+CGG + chr5_4:38389364-38389383 None:intergenic 20.0%
!!! AGTATTTTAACAAAGTAAAC+AGG + chr5_4:38389771-38389790 None:intergenic 20.0%
!!! AATTTTGTGCAAAATGAAAT+TGG - chr5_4:38389852-38389871 Msa0850030:CDS 20.0%
!!! GAAAGACAAATATATGATTT+TGG - chr5_4:38389911-38389930 Msa0850030:CDS 20.0%
!! TTAAAGTAATGAATATTACC+TGG - chr5_4:38390056-38390075 Msa0850030:CDS 20.0%
!! ATAGCTATAAGTAAAATCAA+AGG - chr5_4:38390224-38390243 Msa0850030:five_prime_UTR 20.0%
!! TGATGTACACAATATTATAA+GGG - chr5_4:38390257-38390276 Msa0850030:five_prime_UTR 20.0%
!!! TGAAAGTTTAGAAGTTTTGT+TGG + chr5_4:38387849-38387868 None:intergenic 25.0%
!!! ACACTAGTTTTTGATTTTGA+AGG - chr5_4:38387875-38387894 Msa0850030:three_prime_UTR 25.0%
!! ATGGTTTTCTTAACCTTTTT+TGG - chr5_4:38388040-38388059 Msa0850030:exon 25.0%
!! GTCTTTTCTTATACCAAAAA+AGG + chr5_4:38388056-38388075 None:intergenic 25.0%
! GTATAAGAAAAGACACTTTA+AGG - chr5_4:38388062-38388081 Msa0850030:CDS 25.0%
! TAGTAAGTTACCAAGAATAA+TGG - chr5_4:38388177-38388196 Msa0850030:CDS 25.0%
! GTTTCTTGAAAGACTATTTA+GGG - chr5_4:38388206-38388225 Msa0850030:CDS 25.0%
! TTTCTTGAAAGACTATTTAG+GGG - chr5_4:38388207-38388226 Msa0850030:CDS 25.0%
!!! GAGAATAGTATTGGTATTAT+TGG - chr5_4:38388337-38388356 Msa0850030:CDS 25.0%
!! ATGTTCACACTTTAAAAACA+TGG - chr5_4:38388434-38388453 Msa0850030:CDS 25.0%
! AGCTTAAAAAAAGAAAACGA+CGG + chr5_4:38388503-38388522 None:intergenic 25.0%
! ATAGTGTGTGATATATAAAG+TGG + chr5_4:38389426-38389445 None:intergenic 25.0%
!!! TTAATCGCGTTGTTTTAATA+GGG + chr5_4:38389506-38389525 None:intergenic 25.0%
!! GTTAAGGTTAGTATGTTTTA+TGG - chr5_4:38389687-38389706 Msa0850030:CDS 25.0%
! TTAACAAAGTAAACAGGATT+TGG + chr5_4:38389765-38389784 None:intergenic 25.0%
! ACTTTGTTAAAATACTTGAG+AGG - chr5_4:38389775-38389794 Msa0850030:CDS 25.0%
! AAATGAAATTGGAAAGAGTT+TGG - chr5_4:38389863-38389882 Msa0850030:CDS 25.0%
! AATGAAATTGGAAAGAGTTT+GGG - chr5_4:38389864-38389883 Msa0850030:CDS 25.0%
! GTGATGTACACAATATTATA+AGG - chr5_4:38390256-38390275 Msa0850030:five_prime_UTR 25.0%
!! AGGTTGGTTCTTTAAAACAA+AGG + chr5_4:38387794-38387813 None:intergenic 30.0%
! TTTGTTTCTTTCATACCCTT+TGG - chr5_4:38387979-38387998 Msa0850030:three_prime_UTR 30.0%
TCAACTAGGTTTGAAGATTA+TGG - chr5_4:38388021-38388040 Msa0850030:three_prime_UTR 30.0%
ACATCTTCTAAGTAGAACTT+TGG + chr5_4:38388106-38388125 None:intergenic 30.0%
AAGTTCTACTTAGAAGATGT+TGG - chr5_4:38388106-38388125 Msa0850030:CDS 30.0%
TTACCAAGAATAATGGTTGA+AGG - chr5_4:38388184-38388203 Msa0850030:CDS 30.0%
AAACCTTCAACCATTATTCT+TGG + chr5_4:38388190-38388209 None:intergenic 30.0%
GGTTTCTTGAAAGACTATTT+AGG - chr5_4:38388205-38388224 Msa0850030:CDS 30.0%
AGAGACATAAAGTCATACAA+TGG - chr5_4:38388247-38388266 Msa0850030:CDS 30.0%
! TCATACAATGGTTACCTTTT+GGG - chr5_4:38388259-38388278 Msa0850030:CDS 30.0%
ACAAGCAAAGAGAATAGTAT+TGG - chr5_4:38388328-38388347 Msa0850030:CDS 30.0%
TCTGGTCAATATACTCAATA+TGG + chr5_4:38388366-38388385 None:intergenic 30.0%
!!! ACAAGGTTTGGTTTCTTTTT+TGG - chr5_4:38388401-38388420 Msa0850030:CDS 30.0%
TGCATTATTGTCTAATTTCG+CGG - chr5_4:38388643-38388662 Msa0850030:CDS 30.0%
AATTTCGCGGTTTAATACTA+TGG - chr5_4:38388656-38388675 Msa0850030:CDS 30.0%
! TTATGTTAAGTTCTGATGAG+AGG - chr5_4:38389100-38389119 Msa0850030:intron 30.0%
ATTGAAAGAAATAGTCCAAG+TGG + chr5_4:38389240-38389259 None:intergenic 30.0%
! CTCCAATATTAGCATTTTCA+GGG - chr5_4:38389304-38389323 Msa0850030:intron 30.0%
!!! TAATCGCGTTGTTTTAATAG+GGG + chr5_4:38389505-38389524 None:intergenic 30.0%
!!! GTTAATCGCGTTGTTTTAAT+AGG + chr5_4:38389507-38389526 None:intergenic 30.0%
! AGCTAGTGGATATAAATGTT+TGG - chr5_4:38389716-38389735 Msa0850030:CDS 30.0%
GGATTCAAGTAATGAAAAAC+AGG + chr5_4:38389744-38389763 None:intergenic 30.0%
! TTTTGACATGTTTGTGATGT+TGG + chr5_4:38389804-38389823 None:intergenic 30.0%
ACATCACAAACATGTCAAAA+AGG - chr5_4:38389804-38389823 Msa0850030:CDS 30.0%
ACAAAATTAGCAACTTCAAC+AGG + chr5_4:38389840-38389859 None:intergenic 30.0%
TTTGTCTTTCCTAGTCAAAT+TGG + chr5_4:38389901-38389920 None:intergenic 30.0%
!! GACAAATATATGATTTTGGC+AGG - chr5_4:38389915-38389934 Msa0850030:CDS 30.0%
AACTTGCAGTTATTATGACA+AGG + chr5_4:38389973-38389992 None:intergenic 30.0%
!! TGACACACAAATTAACTTGT+AGG + chr5_4:38389997-38390016 None:intergenic 30.0%
AAGTAATGAATATTACCTGG+TGG - chr5_4:38390059-38390078 Msa0850030:CDS 30.0%
AGTAATGAATATTACCTGGT+GGG - chr5_4:38390060-38390079 Msa0850030:CDS 30.0%
!!! AAGTTTTGTTGGCAAGTGAA+AGG + chr5_4:38387838-38387857 None:intergenic 35.0%
AGGAAACTAGCATGAAGTAA+GGG + chr5_4:38387928-38387947 None:intergenic 35.0%
AAGGAAACTAGCATGAAGTA+AGG + chr5_4:38387929-38387948 None:intergenic 35.0%
TTCTTTCATACCCTTTGGTA+TGG - chr5_4:38387984-38388003 Msa0850030:three_prime_UTR 35.0%
!! TTAGTTGGTGAGTATCAACT+AGG - chr5_4:38388007-38388026 Msa0850030:three_prime_UTR 35.0%
! AGAAAAGACACTTTAAGGGT+AGG - chr5_4:38388067-38388086 Msa0850030:CDS 35.0%
TCTACTTAGAAGATGTTGGT+TGG - chr5_4:38388110-38388129 Msa0850030:CDS 35.0%
! CTACTTAGAAGATGTTGGTT+GGG - chr5_4:38388111-38388130 Msa0850030:CDS 35.0%
! GTCATACAATGGTTACCTTT+TGG - chr5_4:38388258-38388277 Msa0850030:CDS 35.0%
GTTTCTCAAACAAACCCAAA+AGG + chr5_4:38388276-38388295 None:intergenic 35.0%
TGCTTTAACATCATAGGAAG+TGG + chr5_4:38388308-38388327 None:intergenic 35.0%
!! GCTTGTTGCTTTAACATCAT+AGG + chr5_4:38388314-38388333 None:intergenic 35.0%
TGTAATGAGGGAAAAGTTTC+TGG + chr5_4:38388384-38388403 None:intergenic 35.0%
! GAAACTTTTCCCTCATTACA+AGG - chr5_4:38388384-38388403 Msa0850030:CDS 35.0%
! TTTTCCCTCATTACAAGGTT+TGG - chr5_4:38388389-38388408 Msa0850030:CDS 35.0%
GAAACCAAACCTTGTAATGA+GGG + chr5_4:38388396-38388415 None:intergenic 35.0%
AGAAACCAAACCTTGTAATG+AGG + chr5_4:38388397-38388416 None:intergenic 35.0%
CATAGTCGCTAATTAACAGA+AGG + chr5_4:38388562-38388581 None:intergenic 35.0%
GAAAGAAAAAGTTGTCACAG+AGG + chr5_4:38388746-38388765 None:intergenic 35.0%
GAATGATTGTCCCAAAATCA+TGG - chr5_4:38388961-38388980 Msa0850030:intron 35.0%
!!! GGCTTAGGAAACAACTTTTT+TGG + chr5_4:38389144-38389163 None:intergenic 35.0%
TCATGGAAAATCAATGGCTT+AGG + chr5_4:38389159-38389178 None:intergenic 35.0%
!!! AAGCCATTGATTTTCCATGA+TGG - chr5_4:38389159-38389178 Msa0850030:intron 35.0%
CTTCCATCATGGAAAATCAA+TGG + chr5_4:38389165-38389184 None:intergenic 35.0%
!! GATTTTCCATGATGGAAGAT+TGG - chr5_4:38389167-38389186 Msa0850030:intron 35.0%
ACTAGTGAAGATGTTACAGT+TGG + chr5_4:38389201-38389220 None:intergenic 35.0%
ATTGGAGCTGATGCATTAAA+AGG + chr5_4:38389291-38389310 None:intergenic 35.0%
! GCTCCAATATTAGCATTTTC+AGG - chr5_4:38389303-38389322 Msa0850030:intron 35.0%
GTCCCTGAAAATGCTAATAT+TGG + chr5_4:38389309-38389328 None:intergenic 35.0%
!!! ATTTTTTTCGTTTTCGGTCG+AGG + chr5_4:38389358-38389377 None:intergenic 35.0%
ATGGAGAAAATGCTTTGTGA+AGG - chr5_4:38389555-38389574 Msa0850030:intron 35.0%
TCAGTTAGTTCAGCAAACAA+AGG + chr5_4:38389642-38389661 None:intergenic 35.0%
! TTTTATGGAACAACAGCTAG+TGG - chr5_4:38389702-38389721 Msa0850030:CDS 35.0%
TCACAAACATGTCAAAAAGG+TGG - chr5_4:38389807-38389826 Msa0850030:CDS 35.0%
! ATTGGAAAGAGTTTGGGATT+TGG - chr5_4:38389870-38389889 Msa0850030:CDS 35.0%
!! ATGATTTTGGCAGGTAATGA+AGG - chr5_4:38389924-38389943 Msa0850030:CDS 35.0%
! TTTTCATCTTGCTGCAGGTA+AGG + chr5_4:38387814-38387833 None:intergenic 40.0%
! AAGGTTTTTCATCTTGCTGC+AGG + chr5_4:38387819-38387838 None:intergenic 40.0%
!! ATGCTAGTTTCCTTTGAAGC+TGG - chr5_4:38387935-38387954 Msa0850030:three_prime_UTR 40.0%
TAGTATTCCACCAGCTTCAA+AGG + chr5_4:38387948-38387967 None:intergenic 40.0%
TACCCTTTGGTATGGTTAGT+TGG - chr5_4:38387992-38388011 Msa0850030:three_prime_UTR 40.0%
CACCAACTAACCATACCAAA+GGG + chr5_4:38387997-38388016 None:intergenic 40.0%
TCACCAACTAACCATACCAA+AGG + chr5_4:38387998-38388017 None:intergenic 40.0%
! AGACACTTTAAGGGTAGGAA+TGG - chr5_4:38388072-38388091 Msa0850030:CDS 40.0%
! TTAGAAGATGTTGGTTGGGA+AGG - chr5_4:38388115-38388134 Msa0850030:CDS 40.0%
ATACTGCGATTCCTGATTGT+AGG - chr5_4:38388938-38388957 Msa0850030:intron 40.0%
TACTGCGATTCCTGATTGTA+GGG - chr5_4:38388939-38388958 Msa0850030:intron 40.0%
GACAATCATTCCCTACAATC+AGG + chr5_4:38388952-38388971 None:intergenic 40.0%
TGATTGTCCCAAAATCATGG+TGG - chr5_4:38388964-38388983 Msa0850030:intron 40.0%
GTTCTGATGAGAGGAGAAAT+TGG - chr5_4:38389109-38389128 Msa0850030:intron 40.0%
TGAAAGCCAATCTTCCATCA+TGG + chr5_4:38389176-38389195 None:intergenic 40.0%
CTTCACTAGTCATGTTCATG+TGG - chr5_4:38389211-38389230 Msa0850030:intron 40.0%
ATGTTCATGTGGTTACCACT+TGG - chr5_4:38389222-38389241 Msa0850030:intron 40.0%
GTGGATCAAGCAAAGTTCTA+TGG + chr5_4:38389407-38389426 None:intergenic 40.0%
TCTCTACAAGTTGTTCCTTC+TGG + chr5_4:38389597-38389616 None:intergenic 40.0%
GGACTAAACCTTAGCAAGTA+AGG + chr5_4:38389621-38389640 None:intergenic 40.0%
! TCCTGTTGCTGTTGATGTTA+AGG - chr5_4:38389671-38389690 Msa0850030:CDS 40.0%
ACCTTAACATCAACAGCAAC+AGG + chr5_4:38389675-38389694 None:intergenic 40.0%
TTGGTTGCACCAATTTGACT+AGG - chr5_4:38389889-38389908 Msa0850030:CDS 40.0%
GAAGTAAGGGAAGAGTGAAG+TGG + chr5_4:38387915-38387934 None:intergenic 45.0%
!! CTAGTTTCCTTTGAAGCTGG+TGG - chr5_4:38387938-38387957 Msa0850030:three_prime_UTR 45.0%
! CGTTTTCGGTCGAGGTTTTA+GGG + chr5_4:38389350-38389369 None:intergenic 45.0%
! TCGTTTTCGGTCGAGGTTTT+AGG + chr5_4:38389351-38389370 None:intergenic 45.0%
CTATATGTGACTGCTGAGAG+TGG + chr5_4:38389456-38389475 None:intergenic 45.0%
TTGAATCCCTGTCTCTTGCT+CGG + chr5_4:38389536-38389555 None:intergenic 45.0%
AGCAAGAGACAGGGATTCAA+TGG - chr5_4:38389536-38389555 Msa0850030:intron 45.0%
AACCTTGTAGTGTGTCCAGA+AGG - chr5_4:38389579-38389598 Msa0850030:intron 45.0%
TTCCTTCTGGACACACTACA+AGG + chr5_4:38389584-38389603 None:intergenic 45.0%
! GTAGAGAGCCTTACTTGCTA+AGG - chr5_4:38389610-38389629 Msa0850030:intron 45.0%
CATCTTGCTGCAGGTAAGGT+TGG + chr5_4:38387810-38387829 None:intergenic 50.0%
! CAGCGTGCCACCATGATTTT+GGG + chr5_4:38388974-38388993 None:intergenic 50.0%
! ACAGCGTGCCACCATGATTT+TGG + chr5_4:38388975-38388994 None:intergenic 50.0%
! TACCTGGTGGGTCAGTAGTG+TGG - chr5_4:38390072-38390091 Msa0850030:CDS 55.0%
GTGGGTCAGTAGTGTGGTGA+GGG - chr5_4:38390078-38390097 Msa0850030:CDS 55.0%
CACCACACTACTGACCCACC+AGG + chr5_4:38390077-38390096 None:intergenic 60.0%
GGTGGGTCAGTAGTGTGGTG+AGG - chr5_4:38390077-38390096 Msa0850030:CDS 60.0%
GCGCGACCGAGCAAGAGACA+GGG - chr5_4:38389527-38389546 Msa0850030:intron 65.0%
CGCGCGACCGAGCAAGAGAC+AGG - chr5_4:38389526-38389545 Msa0850030:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 38387742 38390280 38387742 ID=Msa0850030;Name=Msa0850030
chr5_4 mRNA 38387742 38390280 38387742 ID=Msa0850030-mRNA-1;Parent=Msa0850030;Name=Msa0850030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=85|1|1|1|1|1|2|300|489
chr5_4 exon 38387742 38388932 38387742 ID=Msa0850030-mRNA-1:exon:12351;Parent=Msa0850030-mRNA-1
chr5_4 exon 38389617 38390280 38389617 ID=Msa0850030-mRNA-1:exon:12350;Parent=Msa0850030-mRNA-1
chr5_4 five_prime_UTR 38390196 38390280 38390196 ID=Msa0850030-mRNA-1:five_prime_utr;Parent=Msa0850030-mRNA-1
chr5_4 CDS 38389617 38390195 38389617 ID=Msa0850030-mRNA-1:cds;Parent=Msa0850030-mRNA-1
chr5_4 CDS 38388042 38388932 38388042 ID=Msa0850030-mRNA-1:cds;Parent=Msa0850030-mRNA-1
chr5_4 three_prime_UTR 38387742 38388041 38387742 ID=Msa0850030-mRNA-1:three_prime_utr;Parent=Msa0850030-mRNA-1
Gene Sequence

>Msa0850030

ATGAAAAACCTTTCACTTGCCAACAAAACTTCTAAACTTTCAAATAAAACACTAGTTTTTGATTTTGAAGGCACTCTTCTAAGATCCACTTCACTCTTCCCTTACTTCATGCTAGTTTCCTTTGAAGCTGGTGGAATACTAAGATCTATCATTTTGTTTCTTTCATACCCTTTGGTATGGTTAGTTGGTGAGTATCAACTAGGTTTGAAGATTATGGTTTTCTTAACCTTTTTTGGTATAAGAAAAGACACTTTAAGGGTAGGAATGGCTGTTCTTCCAAAGTTCTACTTAGAAGATGTTGGTTGGGAAGGATTTGAAGCTGTGATGTGTTGTGAGAGAAAAGTTGCATCTAGTAAGTTACCAAGAATAATGGTTGAAGGTTTCTTGAAAGACTATTTAGGGGTTGAAGCTATTGTAGCAAGAGACATAAAGTCATACAATGGTTACCTTTTGGGTTTGTTTGAGAAACATAAAACAACCACTTCCTATGATGTTAAAGCAACAAGCAAAGAGAATAGTATTGGTATTATTGGTAGCCATATTGAGTATATTGACCAGAAACTTTTCCCTCATTACAAGAAGATTTGCTTTATGTTAAGTTCTGATGAGAGGAGAAATTGGCGTGTATTACCAAAAAAGTTGTTTCCTAAGCCATTGATTTTCCATGATGGAAGATTGGCTTTCATGCCAACTGTAACATCTTCACTAGTCATGTTCATGTGGTTACCACTTGGACTATTTCTTTCAATTTTCAGAATAACATTTGGAGTTTCATTACCTTTTAATGCATCAGCTCCAATATTAGCATTTTCAGGGACAAGAAGCACAATTTCAAGCCCTAAAACCTCGACCGAAAACGAAAAAAATCAAAAGAATATGCTTTATGTGTGTAACCATAGAACTTTGCTTGATCCACTTTATATATCACACACTATAAACAAACCACTCTCAGCAGTCACATATAGCATGAGCAGAGTCAATGAGATTATTTCCCCTATTAAAACAACGCGATTAACGCGCGACCGAGCAAGAGACAGGGATTCAATGGAGAAAATGCTTTGTGAAGGAAACCTTGTAGTGTGTCCAGAAGGAACAACTTGTAGAGAGCCTTACTTGCTAAGGTTTAGTCCTTTGTTTGCTGAACTAACTGATGATATTGTTCCTGTTGCTGTTGATGTTAAGGTTAGTATGTTTTATGGAACAACAGCTAGTGGATATAAATGTTTGGATCCTGTTTTTCATTACTTGAATCCAAATCCTGTTTACTTTGTTAAAATACTTGAGAGGATACCAACATCACAAACATGTCAAAAAGGTGGAAAATCTCCTGTTGAAGTTGCTAATTTTGTGCAAAATGAAATTGGAAAGAGTTTGGGATTTGGTTGCACCAATTTGACTAGGAAAGACAAATATATGATTTTGGCAGGTAATGAAGGTGTGAGTAGCAATAACAAACAATCCTTGTCATAA
Protein sequence

>Msa0850030

MKNLSLANKTSKLSNKTLVFDFEGTLLRSTSLFPYFMLVSFEAGGILRSIILFLSYPLVWLVGEYQLGLKIMVFLTFFGIRKDTLRVGMAVLPKFYLEDVGWEGFEAVMCCERKVASSKLPRIMVEGFLKDYLGVEAIVARDIKSYNGYLLGLFEKHKTTTSYDVKATSKENSIGIIGSHIEYIDQKLFPHYKKICFMLSSDERRNWRVLPKKLFPKPLIFHDGRLAFMPTVTSSLVMFMWLPLGLFLSIFRITFGVSLPFNASAPILAFSGTRSTISSPKTSTENEKNQKNMLYVCNHRTLLDPLYISHTINKPLSAVTYSMSRVNEIISPIKTTRLTRDRARDRDSMEKMLCEGNLVVCPEGTTCREPYLLRFSPLFAELTDDIVPVAVDVKVSMFYGTTASGYKCLDPVFHYLNPNPVYFVKILERIPTSQTCQKGGKSPVEVANFVQNEIGKSLGFGCTNLTRKDKYMILAGNEGVSSNNKQSLS*