AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0809460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809460 MtrunA17_Chr5g0425071 96.933 489 15 0 1 489 1 489 0.0 981
Msa0809460 MtrunA17_Chr1g0194391 51.943 489 224 8 5 488 54 536 2.92e-171 493
Msa0809460 MtrunA17_Chr5g0443341 49.474 475 221 8 15 480 64 528 3.13e-158 460
Msa0809460 MtrunA17_Chr3g0096481 49.167 480 226 9 15 485 59 529 5.23e-155 451
Msa0809460 MtrunA17_Chr8g0348781 42.593 486 257 8 8 479 18 495 1.01e-121 365
Msa0809460 MtrunA17_Chr4g0005991 42.300 487 266 8 10 487 15 495 4.52e-121 363
Msa0809460 MtrunA17_Chr1g0170421 41.010 495 272 11 8 487 45 534 1.70e-120 365
Msa0809460 MtrunA17_Chr5g0435581 40.125 481 263 9 17 482 3 473 1.26e-116 352
Msa0809460 MtrunA17_Chr7g0241831 39.200 500 276 7 2 486 10 496 1.59e-116 352
Msa0809460 MtrunA17_Chr3g0096591 55.311 273 115 3 210 479 1 269 2.79e-109 325
Msa0809460 MtrunA17_Chr3g0096581 40.278 144 84 2 15 157 63 205 2.72e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0809460 AT4G01950.1 51.653 484 221 10 11 484 40 520 2.46e-169 488
Msa0809460 AT4G01950.2 51.653 484 221 10 11 484 68 548 9.77e-169 487
Msa0809460 AT1G02390.1 50.204 490 221 8 8 480 43 526 4.49e-165 477
Msa0809460 AT1G06520.1 47.205 483 232 10 17 480 105 583 1.02e-149 440
Msa0809460 AT2G38110.1 42.227 476 260 9 14 479 22 492 2.16e-119 359
Msa0809460 AT3G11430.1 39.959 483 267 9 14 482 9 482 2.96e-119 359
Msa0809460 AT5G06090.1 40.329 486 271 12 9 482 2 480 2.01e-118 357
Msa0809460 AT1G01610.1 42.380 479 263 8 17 485 23 498 8.86e-117 353
Msa0809460 AT4G00400.1 41.949 472 261 7 17 479 23 490 5.65e-111 338
Msa0809460 AT3G11325.2 32.321 461 212 10 26 482 9 373 3.32e-72 234
Msa0809460 AT3G11325.1 32.321 461 212 10 26 482 6 370 3.62e-72 234

Find 93 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCTTGAAAGACTATTT+AGG 0.195935 5_3:-36137903 Msa0809460:CDS
TGTAATGAGGGAAAAGTTTC+TGG 0.203098 5_3:+36137727 None:intergenic
GTATAAGAAAAGACACTTTA+AGG 0.207106 5_3:-36138046 Msa0809460:CDS
GAGAATAGTATTGGTATTAT+TGG 0.218296 5_3:-36137771 Msa0809460:CDS
TCAACTAGGTTTGAAGATTA+TGG 0.218722 5_3:-36138087 Msa0809460:CDS
GAAAGACAAATATATGATTT+TGG 0.225097 5_3:-36136199 Msa0809460:CDS
ATTAAAACTAAGATAGAAAT+AGG 0.231887 5_3:+36136076 None:intergenic
GTCATACAATGGTTACCTTT+TGG 0.239819 5_3:-36137850 Msa0809460:CDS
AAATGAAATTGGAAAGAGTT+TGG 0.246873 5_3:-36136247 Msa0809460:CDS
AAACCTTCAACCATTATTCT+TGG 0.248292 5_3:+36137921 None:intergenic
AATTGTAGTTATACTTGTTT+TGG 0.249787 5_3:+36138344 None:intergenic
GTGATGTACACAATATTATA+AGG 0.272620 5_3:-36135854 Msa0809460:three_prime_UTR
TTAACAAAGTAAACAGGATT+TGG 0.272628 5_3:+36136348 None:intergenic
GTCCCTGAAAATGCTAATAT+TGG 0.280666 5_3:+36136804 None:intergenic
TCTCTACATGTTGTTCCTTC+TGG 0.291482 5_3:+36136516 None:intergenic
ATTGGAGCTGATGCATTAAA+AGG 0.293102 5_3:+36136822 None:intergenic
TCTGGTCAATATACTCAATA+TGG 0.319173 5_3:+36137745 None:intergenic
TTTGTCTTTCCTAGTCAAAT+TGG 0.322666 5_3:+36136212 None:intergenic
AATGAAATTGGAAAGAGTTT+GGG 0.331774 5_3:-36136246 Msa0809460:CDS
TTTGTTTCTTTCATACCCTT+TGG 0.336221 5_3:-36138129 Msa0809460:CDS
ACATCACAAACATGTCAAAA+AGG 0.340009 5_3:-36136306 Msa0809460:CDS
GTTCTGATGAGAGGAGAAAT+TGG 0.353548 5_3:-36137001 Msa0809460:CDS
GTGGATCAAGCAAAGTTCTA+TGG 0.367532 5_3:+36136706 None:intergenic
GTTTCTTGAAAGACTATTTA+GGG 0.373176 5_3:-36137902 Msa0809460:CDS
TACCCTTTGGTATGGTTAGT+TGG 0.374543 5_3:-36138116 Msa0809460:CDS
TCCTGTTGCTGTTGATGTTA+AGG 0.382374 5_3:-36136439 Msa0809460:CDS
TGACACACAAATTAACTTGT+AGG 0.389095 5_3:+36136116 None:intergenic
GCTTGTTGCTTTAACATCAT+AGG 0.399072 5_3:+36137797 None:intergenic
ATTGGAAAGAGTTTGGGATT+TGG 0.401189 5_3:-36136240 Msa0809460:CDS
TATAAGAAAAGACACTTTAA+GGG 0.412950 5_3:-36138045 Msa0809460:CDS
ACAAAATTAGCAACTTCAAC+AGG 0.421254 5_3:+36136273 None:intergenic
TTCTTTCATACCCTTTGGTA+TGG 0.423298 5_3:-36138124 Msa0809460:CDS
TAGTAAGTTACCAAGAATAA+TGG 0.425255 5_3:-36137931 Msa0809460:CDS
ACCTTAACATCAACAGCAAC+AGG 0.427108 5_3:+36136438 None:intergenic
GGACTAAACCTTAGCAAGTA+AGG 0.432275 5_3:+36136492 None:intergenic
CATCTTGCTGCAGGTAAGGT+TGG 0.435384 5_3:+36138301 None:intergenic
ATGCTAGTTTCCTTTGAAGC+TGG 0.441903 5_3:-36138173 Msa0809460:CDS
CTACTTAGAAGATGTTGGTT+GGG 0.444813 5_3:-36137997 Msa0809460:CDS
AGCTAGTGGATATAAATGTT+TGG 0.447892 5_3:-36136394 Msa0809460:CDS
CGCGCGATCGAGCAAGAGAC+AGG 0.452896 5_3:-36136584 Msa0809460:CDS
TTACCAAGAATAATGGTTGA+AGG 0.459458 5_3:-36137924 Msa0809460:CDS
TCATGGAAAATCAATGGCTT+AGG 0.460797 5_3:+36136954 None:intergenic
AAGGAAACTAGCATGAAGTA+AGG 0.462736 5_3:+36138182 None:intergenic
TGATGTACACAATATTATAA+GGG 0.467952 5_3:-36135853 Msa0809460:three_prime_UTR
TTAGAAGATGTTGGTTGGGA+AGG 0.468636 5_3:-36137993 Msa0809460:CDS
ACATCTTCTAAGTAGAACTT+TGG 0.478280 5_3:+36138005 None:intergenic
AGCAAGAGACAGGGATTCAA+TGG 0.479144 5_3:-36136574 Msa0809460:CDS
CTAGTTTCCTTTGAAGCTGG+TGG 0.484232 5_3:-36138170 Msa0809460:CDS
CTTCCATCATGGAAAATCAA+TGG 0.487517 5_3:+36136948 None:intergenic
ATAGTGTGTGATATATAAAG+TGG 0.489764 5_3:+36136687 None:intergenic
TGCTTTAACATCATAGGAAG+TGG 0.493418 5_3:+36137803 None:intergenic
TAGTATTCCACCAGCTTCAA+AGG 0.493486 5_3:+36138163 None:intergenic
GTTTCTCAAACAAACCCAAA+AGG 0.507148 5_3:+36137835 None:intergenic
ACAAGCAAAGAGAATAGTAT+TGG 0.508669 5_3:-36137780 Msa0809460:CDS
TCTACTTAGAAGATGTTGGT+TGG 0.515294 5_3:-36137998 Msa0809460:CDS
GTGGGTCAGTAGTGTGGTGA+GGG 0.516042 5_3:-36136032 Msa0809460:three_prime_UTR
CACCAACTAACCATACCAAA+GGG 0.528093 5_3:+36138114 None:intergenic
AGACACTTTAAGGGTAGGAA+TGG 0.534443 5_3:-36138036 Msa0809460:CDS
AGAAACCAAACCTTGTAATG+AGG 0.540345 5_3:+36137714 None:intergenic
GTAGAGAGCCTTACTTGCTA+AGG 0.541715 5_3:-36136500 Msa0809460:CDS
TGAAAGCCAATCTTCCATCA+TGG 0.549956 5_3:+36136937 None:intergenic
GAAACCAAACCTTGTAATGA+GGG 0.554140 5_3:+36137715 None:intergenic
AAGTTCTACTTAGAAGATGT+TGG 0.556652 5_3:-36138002 Msa0809460:CDS
ATGGAGAAAATGCTTTGTGA+AGG 0.565042 5_3:-36136555 Msa0809460:CDS
TTGGTTGCACCAATTTGACT+AGG 0.569693 5_3:-36136221 Msa0809460:CDS
TTATGTTAAGTTCTGATGAG+AGG 0.571837 5_3:-36137010 Msa0809460:CDS
GGTGGGTCAGTAGTGTGGTG+AGG 0.575100 5_3:-36136033 Msa0809460:three_prime_UTR
AACCTTGTGGTATGTCCAGA+AGG 0.576105 5_3:-36136531 Msa0809460:CDS
AGAAAAGACACTTTAAGGGT+AGG 0.579822 5_3:-36138041 Msa0809460:CDS
AGGTTGGTTCTTTAAAACAA+AGG 0.587296 5_3:+36138317 None:intergenic
ATGTTCATGTGGTTACCACT+TGG 0.589495 5_3:-36136888 Msa0809460:CDS
AGGAAACTAGCATGAAGTAA+GGG 0.591307 5_3:+36138183 None:intergenic
AGTAATGAATATTACCTGGT+GGG 0.595121 5_3:-36136050 Msa0809460:three_prime_UTR
TCACCAACTAACCATACCAA+AGG 0.596402 5_3:+36138113 None:intergenic
ATTGAAAGAAATAGTCCAAG+TGG 0.598959 5_3:+36136873 None:intergenic
AACTTGCAGTTATTATGACA+AGG 0.600625 5_3:+36136140 None:intergenic
TTAAAGTAATGAATATTACC+TGG 0.601181 5_3:-36136054 Msa0809460:three_prime_UTR
TTAGTTGGTGAGTATCAACT+AGG 0.603020 5_3:-36138101 Msa0809460:CDS
ACTAGTGAAGATGTTACAGT+TGG 0.610755 5_3:+36136912 None:intergenic
CTTCACTAGTCATGTTCATG+TGG 0.612621 5_3:-36136899 Msa0809460:CDS
ATAGCTATAAGTAAAATCAA+AGG 0.615844 5_3:-36135886 Msa0809460:three_prime_UTR
ACTTTGTTAAAATACTTGAG+AGG 0.622036 5_3:-36136335 Msa0809460:CDS
AAGTAATGAATATTACCTGG+TGG 0.625285 5_3:-36136051 Msa0809460:three_prime_UTR
CACCACACTACTGACCCACC+AGG 0.632024 5_3:+36136036 None:intergenic
GAAGTAAGGGAAGAGTGAAG+TGG 0.637079 5_3:+36138196 None:intergenic
CTATATGTGACTGCTGAGAG+TGG 0.637208 5_3:+36136657 None:intergenic
TTCCTTCTGGACATACCACA+AGG 0.637353 5_3:+36136529 None:intergenic
GCGCGATCGAGCAAGAGACA+GGG 0.637383 5_3:-36136583 Msa0809460:CDS
TTTCTTGAAAGACTATTTAG+GGG 0.640361 5_3:-36137901 Msa0809460:CDS
TCAGTTAGCTCAGCAAACAA+AGG 0.654655 5_3:+36136471 None:intergenic
AGAGACATAAAGTCATACAA+TGG 0.657033 5_3:-36137861 Msa0809460:CDS
TACCTGGTGGGTCAGTAGTG+TGG 0.678698 5_3:-36136038 Msa0809460:three_prime_UTR
GCTTTGTGAAGGAAACCTTG+TGG 0.758293 5_3:-36136544 Msa0809460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAAAAATTGTAATAATTT+TGG - chr5_3:36138248-36138267 Msa0809460:CDS 10.0%
!!! AGCTTTTGTTAAAATTATTA+TGG - chr5_3:36136643-36136662 Msa0809460:CDS 15.0%
!!! TTTTTAGACAAAAAAAAAGT+TGG + chr5_3:36137141-36137160 None:intergenic 15.0%
!! ATTAAAACTAAGATAGAAAT+AGG + chr5_3:36138146-36138165 None:intergenic 15.0%
!!! ATAATTTTGGATGTTTATAT+AGG - chr5_3:36138261-36138280 Msa0809460:CDS 15.0%
!!! AATTGTAGTTATACTTGTTT+TGG + chr5_3:36135878-36135897 None:intergenic 20.0%
!! TATAAGAAAAGACACTTTAA+GGG - chr5_3:36136174-36136193 Msa0809460:CDS 20.0%
!!! TCAATTTTCAGAATAACATT+TGG - chr5_3:36137364-36137383 Msa0809460:intron 20.0%
!!! CTTTTGATTTTTTTCGTTTT+CGG + chr5_3:36137473-36137492 None:intergenic 20.0%
!!! AGTATTTTAACAAAGTAAAC+AGG + chr5_3:36137880-36137899 None:intergenic 20.0%
!!! AATTTTGTGCAAAATGAAAT+TGG - chr5_3:36137961-36137980 Msa0809460:CDS 20.0%
!!! GAAAGACAAATATATGATTT+TGG - chr5_3:36138020-36138039 Msa0809460:CDS 20.0%
!! TTAAAGTAATGAATATTACC+TGG - chr5_3:36138165-36138184 Msa0809460:CDS 20.0%
!! ATAGCTATAAGTAAAATCAA+AGG - chr5_3:36138333-36138352 Msa0809460:five_prime_UTR 20.0%
!!! TGAAAGTTTAGAAGTTTTGT+TGG + chr5_3:36135960-36135979 None:intergenic 25.0%
!!! ACACTAGTTTTTGATTTTGA+AGG - chr5_3:36135986-36136005 Msa0809460:three_prime_UTR 25.0%
!! ATGGTTTTCTTAACCTTTTT+TGG - chr5_3:36136151-36136170 Msa0809460:exon 25.0%
!! GTCTTTTCTTATACCAAAAA+AGG + chr5_3:36136167-36136186 None:intergenic 25.0%
! GTATAAGAAAAGACACTTTA+AGG - chr5_3:36136173-36136192 Msa0809460:CDS 25.0%
! TAGTAAGTTACCAAGAATAA+TGG - chr5_3:36136288-36136307 Msa0809460:CDS 25.0%
! GTTTCTTGAAAGACTATTTA+GGG - chr5_3:36136317-36136336 Msa0809460:CDS 25.0%
! TTTCTTGAAAGACTATTTAG+GGG - chr5_3:36136318-36136337 Msa0809460:CDS 25.0%
!!! GAGAATAGTATTGGTATTAT+TGG - chr5_3:36136448-36136467 Msa0809460:CDS 25.0%
! AGCTTAAAAAAAGAAAACGA+CGG + chr5_3:36136614-36136633 None:intergenic 25.0%
! ATAGTGTGTGATATATAAAG+TGG + chr5_3:36137535-36137554 None:intergenic 25.0%
!! GTTAAGGTTAGTATGTTTTA+TGG - chr5_3:36137796-36137815 Msa0809460:CDS 25.0%
! TTAACAAAGTAAACAGGATT+TGG + chr5_3:36137874-36137893 None:intergenic 25.0%
! ACTTTGTTAAAATACTTGAG+AGG - chr5_3:36137884-36137903 Msa0809460:CDS 25.0%
! AAATGAAATTGGAAAGAGTT+TGG - chr5_3:36137972-36137991 Msa0809460:CDS 25.0%
! AATGAAATTGGAAAGAGTTT+GGG - chr5_3:36137973-36137992 Msa0809460:CDS 25.0%
! GTGATGTACACAATATTATA+AGG - chr5_3:36138365-36138384 Msa0809460:five_prime_UTR 25.0%
!! AGGTTGGTTCTTTAAAACAA+AGG + chr5_3:36135905-36135924 None:intergenic 30.0%
! TTTGTTTCTTTCATACCCTT+TGG - chr5_3:36136090-36136109 Msa0809460:three_prime_UTR 30.0%
TCAACTAGGTTTGAAGATTA+TGG - chr5_3:36136132-36136151 Msa0809460:three_prime_UTR 30.0%
ACATCTTCTAAGTAGAACTT+TGG + chr5_3:36136217-36136236 None:intergenic 30.0%
AAGTTCTACTTAGAAGATGT+TGG - chr5_3:36136217-36136236 Msa0809460:CDS 30.0%
TTACCAAGAATAATGGTTGA+AGG - chr5_3:36136295-36136314 Msa0809460:CDS 30.0%
AAACCTTCAACCATTATTCT+TGG + chr5_3:36136301-36136320 None:intergenic 30.0%
GGTTTCTTGAAAGACTATTT+AGG - chr5_3:36136316-36136335 Msa0809460:CDS 30.0%
AGAGACATAAAGTCATACAA+TGG - chr5_3:36136358-36136377 Msa0809460:CDS 30.0%
! TCATACAATGGTTACCTTTT+GGG - chr5_3:36136370-36136389 Msa0809460:CDS 30.0%
ACAAGCAAAGAGAATAGTAT+TGG - chr5_3:36136439-36136458 Msa0809460:CDS 30.0%
TCTGGTCAATATACTCAATA+TGG + chr5_3:36136477-36136496 None:intergenic 30.0%
!!! ACAAGGTTTGGTTTCTTTTT+TGG - chr5_3:36136512-36136531 Msa0809460:CDS 30.0%
TGTTCATAGTCGCTAATTAA+CGG + chr5_3:36136677-36136696 None:intergenic 30.0%
TGCATTATTGTCTAATTTCG+CGG - chr5_3:36136754-36136773 Msa0809460:CDS 30.0%
AATTTCGCGGTTTAATACTA+TGG - chr5_3:36136767-36136786 Msa0809460:CDS 30.0%
! TTATGTTAAGTTCTGATGAG+AGG - chr5_3:36137209-36137228 Msa0809460:intron 30.0%
ATTGAAAGAAATAGTCCAAG+TGG + chr5_3:36137349-36137368 None:intergenic 30.0%
! CTCCAATATTAGCATTTTCA+GGG - chr5_3:36137413-36137432 Msa0809460:intron 30.0%
!!! TTAATCGCGTTGTTTTAATG+GGG + chr5_3:36137615-36137634 None:intergenic 30.0%
!!! GTTAATCGCGTTGTTTTAAT+GGG + chr5_3:36137616-36137635 None:intergenic 30.0%
! AGCTAGTGGATATAAATGTT+TGG - chr5_3:36137825-36137844 Msa0809460:CDS 30.0%
GGATTCAAGTAATGAAAAAC+AGG + chr5_3:36137853-36137872 None:intergenic 30.0%
! TTTTGACATGTTTGTGATGT+TGG + chr5_3:36137913-36137932 None:intergenic 30.0%
ACATCACAAACATGTCAAAA+AGG - chr5_3:36137913-36137932 Msa0809460:CDS 30.0%
ACAAAATTAGCAACTTCAAC+AGG + chr5_3:36137949-36137968 None:intergenic 30.0%
TTTGTCTTTCCTAGTCAAAT+TGG + chr5_3:36138010-36138029 None:intergenic 30.0%
!! GACAAATATATGATTTTGGC+AGG - chr5_3:36138024-36138043 Msa0809460:CDS 30.0%
AACTTGCAGTTATTATGACA+AGG + chr5_3:36138082-36138101 None:intergenic 30.0%
!! TGACACACAAATTAACTTGT+AGG + chr5_3:36138106-36138125 None:intergenic 30.0%
AAGTAATGAATATTACCTGG+TGG - chr5_3:36138168-36138187 Msa0809460:CDS 30.0%
AGTAATGAATATTACCTGGT+GGG - chr5_3:36138169-36138188 Msa0809460:CDS 30.0%
!!! AAGTTTTGTTGGCAAGTGAA+AGG + chr5_3:36135949-36135968 None:intergenic 35.0%
AGGAAACTAGCATGAAGTAA+GGG + chr5_3:36136039-36136058 None:intergenic 35.0%
AAGGAAACTAGCATGAAGTA+AGG + chr5_3:36136040-36136059 None:intergenic 35.0%
TTCTTTCATACCCTTTGGTA+TGG - chr5_3:36136095-36136114 Msa0809460:three_prime_UTR 35.0%
!! TTAGTTGGTGAGTATCAACT+AGG - chr5_3:36136118-36136137 Msa0809460:three_prime_UTR 35.0%
! AGAAAAGACACTTTAAGGGT+AGG - chr5_3:36136178-36136197 Msa0809460:CDS 35.0%
TCTACTTAGAAGATGTTGGT+TGG - chr5_3:36136221-36136240 Msa0809460:CDS 35.0%
! CTACTTAGAAGATGTTGGTT+GGG - chr5_3:36136222-36136241 Msa0809460:CDS 35.0%
! GTCATACAATGGTTACCTTT+TGG - chr5_3:36136369-36136388 Msa0809460:CDS 35.0%
GTTTCTCAAACAAACCCAAA+AGG + chr5_3:36136387-36136406 None:intergenic 35.0%
TGCTTTAACATCATAGGAAG+TGG + chr5_3:36136419-36136438 None:intergenic 35.0%
!! GCTTGTTGCTTTAACATCAT+AGG + chr5_3:36136425-36136444 None:intergenic 35.0%
TGTAATGAGGGAAAAGTTTC+TGG + chr5_3:36136495-36136514 None:intergenic 35.0%
! GAAACTTTTCCCTCATTACA+AGG - chr5_3:36136495-36136514 Msa0809460:CDS 35.0%
! TTTTCCCTCATTACAAGGTT+TGG - chr5_3:36136500-36136519 Msa0809460:CDS 35.0%
GAAACCAAACCTTGTAATGA+GGG + chr5_3:36136507-36136526 None:intergenic 35.0%
AGAAACCAAACCTTGTAATG+AGG + chr5_3:36136508-36136527 None:intergenic 35.0%
GAATGATTGTCCCAAAATCA+TGG - chr5_3:36137073-36137092 Msa0809460:intron 35.0%
!!! GGCTTAGGAAACAACTTTTT+TGG + chr5_3:36137253-36137272 None:intergenic 35.0%
TCATGGAAAATCAATGGCTT+AGG + chr5_3:36137268-36137287 None:intergenic 35.0%
!!! AAGCCATTGATTTTCCATGA+TGG - chr5_3:36137268-36137287 Msa0809460:intron 35.0%
CTTCCATCATGGAAAATCAA+TGG + chr5_3:36137274-36137293 None:intergenic 35.0%
!! GATTTTCCATGATGGAAGAT+TGG - chr5_3:36137276-36137295 Msa0809460:intron 35.0%
ACTAGTGAAGATGTTACAGT+TGG + chr5_3:36137310-36137329 None:intergenic 35.0%
ATTGGAGCTGATGCATTAAA+AGG + chr5_3:36137400-36137419 None:intergenic 35.0%
! GCTCCAATATTAGCATTTTC+AGG - chr5_3:36137412-36137431 Msa0809460:intron 35.0%
GTCCCTGAAAATGCTAATAT+TGG + chr5_3:36137418-36137437 None:intergenic 35.0%
!!! ATTTTTTTCGTTTTCGGTCG+AGG + chr5_3:36137467-36137486 None:intergenic 35.0%
!!! TAATCGCGTTGTTTTAATGG+GGG + chr5_3:36137614-36137633 None:intergenic 35.0%
!!! CGTTAATCGCGTTGTTTTAA+TGG + chr5_3:36137617-36137636 None:intergenic 35.0%
ATGGAGAAAATGCTTTGTGA+AGG - chr5_3:36137664-36137683 Msa0809460:intron 35.0%
! TTTTATGGAACAACAGCTAG+TGG - chr5_3:36137811-36137830 Msa0809460:CDS 35.0%
TCACAAACATGTCAAAAAGG+TGG - chr5_3:36137916-36137935 Msa0809460:CDS 35.0%
! ATTGGAAAGAGTTTGGGATT+TGG - chr5_3:36137979-36137998 Msa0809460:CDS 35.0%
!! ATGATTTTGGCAGGTAATGA+AGG - chr5_3:36138033-36138052 Msa0809460:CDS 35.0%
! TTTTCATCTTGCTGCAGGTA+AGG + chr5_3:36135925-36135944 None:intergenic 40.0%
! AAGGTTTTTCATCTTGCTGC+AGG + chr5_3:36135930-36135949 None:intergenic 40.0%
!! ATGCTAGTTTCCTTTGAAGC+TGG - chr5_3:36136046-36136065 Msa0809460:three_prime_UTR 40.0%
TAGTATTCCACCAGCTTCAA+AGG + chr5_3:36136059-36136078 None:intergenic 40.0%
TACCCTTTGGTATGGTTAGT+TGG - chr5_3:36136103-36136122 Msa0809460:three_prime_UTR 40.0%
CACCAACTAACCATACCAAA+GGG + chr5_3:36136108-36136127 None:intergenic 40.0%
TCACCAACTAACCATACCAA+AGG + chr5_3:36136109-36136128 None:intergenic 40.0%
! AGACACTTTAAGGGTAGGAA+TGG - chr5_3:36136183-36136202 Msa0809460:CDS 40.0%
! TTAGAAGATGTTGGTTGGGA+AGG - chr5_3:36136226-36136245 Msa0809460:CDS 40.0%
CATAGTCGCTAATTAACGGA+AGG + chr5_3:36136673-36136692 None:intergenic 40.0%
ATACTGCGATTCCTGATTGT+AGG - chr5_3:36137050-36137069 Msa0809460:intron 40.0%
TACTGCGATTCCTGATTGTA+GGG - chr5_3:36137051-36137070 Msa0809460:intron 40.0%
GACAATCATTCCCTACAATC+AGG + chr5_3:36137064-36137083 None:intergenic 40.0%
TGATTGTCCCAAAATCATGG+TGG - chr5_3:36137076-36137095 Msa0809460:intron 40.0%
GTTCTGATGAGAGGAGAAAT+TGG - chr5_3:36137218-36137237 Msa0809460:intron 40.0%
TGAAAGCCAATCTTCCATCA+TGG + chr5_3:36137285-36137304 None:intergenic 40.0%
CTTCACTAGTCATGTTCATG+TGG - chr5_3:36137320-36137339 Msa0809460:intron 40.0%
ATGTTCATGTGGTTACCACT+TGG - chr5_3:36137331-36137350 Msa0809460:intron 40.0%
GTGGATCAAGCAAAGTTCTA+TGG + chr5_3:36137516-36137535 None:intergenic 40.0%
TCTCTACATGTTGTTCCTTC+TGG + chr5_3:36137706-36137725 None:intergenic 40.0%
GGACTAAACCTTAGCAAGTA+AGG + chr5_3:36137730-36137749 None:intergenic 40.0%
TCAGTTAGCTCAGCAAACAA+AGG + chr5_3:36137751-36137770 None:intergenic 40.0%
! TCCTGTTGCTGTTGATGTTA+AGG - chr5_3:36137780-36137799 Msa0809460:CDS 40.0%
ACCTTAACATCAACAGCAAC+AGG + chr5_3:36137784-36137803 None:intergenic 40.0%
TTGGTTGCACCAATTTGACT+AGG - chr5_3:36137998-36138017 Msa0809460:CDS 40.0%
GAAGTAAGGGAAGAGTGAAG+TGG + chr5_3:36136026-36136045 None:intergenic 45.0%
!! CTAGTTTCCTTTGAAGCTGG+TGG - chr5_3:36136049-36136068 Msa0809460:three_prime_UTR 45.0%
! CGTTTTCGGTCGAGGTTTTA+GGG + chr5_3:36137459-36137478 None:intergenic 45.0%
! TCGTTTTCGGTCGAGGTTTT+AGG + chr5_3:36137460-36137479 None:intergenic 45.0%
CTATATGTGACTGCTGAGAG+TGG + chr5_3:36137565-36137584 None:intergenic 45.0%
AGCAAGAGACAGGGATTCAA+TGG - chr5_3:36137645-36137664 Msa0809460:intron 45.0%
GCTTTGTGAAGGAAACCTTG+TGG - chr5_3:36137675-36137694 Msa0809460:intron 45.0%
AACCTTGTGGTATGTCCAGA+AGG - chr5_3:36137688-36137707 Msa0809460:intron 45.0%
TTCCTTCTGGACATACCACA+AGG + chr5_3:36137693-36137712 None:intergenic 45.0%
! GTAGAGAGCCTTACTTGCTA+AGG - chr5_3:36137719-36137738 Msa0809460:intron 45.0%
CATCTTGCTGCAGGTAAGGT+TGG + chr5_3:36135921-36135940 None:intergenic 50.0%
! CAGCGTGCCACCATGATTTT+GGG + chr5_3:36137086-36137105 None:intergenic 50.0%
! ACAGCGTGCCACCATGATTT+TGG + chr5_3:36137087-36137106 None:intergenic 50.0%
! TACCTGGTGGGTCAGTAGTG+TGG - chr5_3:36138181-36138200 Msa0809460:CDS 55.0%
GTGGGTCAGTAGTGTGGTGA+GGG - chr5_3:36138187-36138206 Msa0809460:CDS 55.0%
GCGCGATCGAGCAAGAGACA+GGG - chr5_3:36137636-36137655 Msa0809460:intron 60.0%
CACCACACTACTGACCCACC+AGG + chr5_3:36138186-36138205 None:intergenic 60.0%
GGTGGGTCAGTAGTGTGGTG+AGG - chr5_3:36138186-36138205 Msa0809460:CDS 60.0%
CGCGCGATCGAGCAAGAGAC+AGG - chr5_3:36137635-36137654 Msa0809460:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 36135853 36138388 36135853 ID=Msa0809460;Name=Msa0809460
chr5_3 mRNA 36135853 36138388 36135853 ID=Msa0809460-mRNA-1;Parent=Msa0809460;Name=Msa0809460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=85|1|1|1|1|1|2|299|489
chr5_3 exon 36135853 36137042 36135853 ID=Msa0809460-mRNA-1:exon:11294;Parent=Msa0809460-mRNA-1
chr5_3 exon 36137725 36138388 36137725 ID=Msa0809460-mRNA-1:exon:11293;Parent=Msa0809460-mRNA-1
chr5_3 five_prime_UTR 36138304 36138388 36138304 ID=Msa0809460-mRNA-1:five_prime_utr;Parent=Msa0809460-mRNA-1
chr5_3 CDS 36137725 36138303 36137725 ID=Msa0809460-mRNA-1:cds;Parent=Msa0809460-mRNA-1
chr5_3 CDS 36136152 36137042 36136152 ID=Msa0809460-mRNA-1:cds;Parent=Msa0809460-mRNA-1
chr5_3 three_prime_UTR 36135853 36136151 36135853 ID=Msa0809460-mRNA-1:three_prime_utr;Parent=Msa0809460-mRNA-1
Gene Sequence

>Msa0809460

ATGAAAAACCTTTCACTTGCCAACAAAACTTCTAAACTTTCAAATAAAACACTAGTTTTTGATTTTGAAGGCACTCTTCTAAGATCCACTTCACTCTTCCCTTACTTCATGCTAGTTTCCTTTGAAGCTGGTGGAATACTAAGATCTATCATTTTGTTTCTTTCATACCCTTTGGTATGGTTAGTTGGTGAGTATCAACTAGGTTTGAAGATTATGGTTTTCTTAACCTTTTTTGGTATAAGAAAAGACACTTTAAGGGTAGGAATGGCTGTTCTTCCAAAGTTCTACTTAGAAGATGTTGGTTGGGAAGGATTTGAAGCTGTGATGTGTTGTGAGAGAAAAGTTGCATCTAGTAAGTTACCAAGAATAATGGTTGAAGGTTTCTTGAAAGACTATTTAGGGGTTGAAGCTATTGTAGCAAGAGACATAAAGTCATACAATGGTTACCTTTTGGGTTTGTTTGAGAAACATAAAACAACCACTTCCTATGATGTTAAAGCAACAAGCAAAGAGAATAGTATTGGTATTATTGGTAGCCATATTGAGTATATTGACCAGAAACTTTTCCCTCATTACAAGAAGATTTGCTTTATGTTAAGTTCTGATGAGAGGAGAAATTGGCGTGTATTACCAAAAAAGTTGTTTCCTAAGCCATTGATTTTCCATGATGGAAGATTGGCTTTCATGCCAACTGTAACATCTTCACTAGTCATGTTCATGTGGTTACCACTTGGACTATTTCTTTCAATTTTCAGAATAACATTTGGAGTTTCATTGCCTTTTAATGCATCAGCTCCAATATTAGCATTTTCAGGGACAAGAAGCACAATTTCAAGCCCTAAAACCTCGACCGAAAACGAAAAAAATCAAAAGAATATGCTTTATGTGTGTAACCATAGAACTTTGCTTGATCCACTTTATATATCACACACTATAAACAAACCACTCTCAGCAGTCACATATAGCATGAGCAGAGTCAATGAGATTATTTCCCCCATTAAAACAACGCGATTAACGCGCGATCGAGCAAGAGACAGGGATTCAATGGAGAAAATGCTTTGTGAAGGAAACCTTGTGGTATGTCCAGAAGGAACAACATGTAGAGAGCCTTACTTGCTAAGGTTTAGTCCTTTGTTTGCTGAGCTAACTGATGATATTGTTCCTGTTGCTGTTGATGTTAAGGTTAGTATGTTTTATGGAACAACAGCTAGTGGATATAAATGTTTGGATCCTGTTTTTCATTACTTGAATCCAAATCCTGTTTACTTTGTTAAAATACTTGAGAGGATACCAACATCACAAACATGTCAAAAAGGTGGAAAATCTCCTGTTGAAGTTGCTAATTTTGTGCAAAATGAAATTGGAAAGAGTTTGGGATTTGGTTGCACCAATTTGACTAGGAAAGACAAATATATGATTTTGGCAGGTAATGAAGGTGTGAGTAGCAATAACAAACAATCCTTGTCATAA
Protein sequence

>Msa0809460

MKNLSLANKTSKLSNKTLVFDFEGTLLRSTSLFPYFMLVSFEAGGILRSIILFLSYPLVWLVGEYQLGLKIMVFLTFFGIRKDTLRVGMAVLPKFYLEDVGWEGFEAVMCCERKVASSKLPRIMVEGFLKDYLGVEAIVARDIKSYNGYLLGLFEKHKTTTSYDVKATSKENSIGIIGSHIEYIDQKLFPHYKKICFMLSSDERRNWRVLPKKLFPKPLIFHDGRLAFMPTVTSSLVMFMWLPLGLFLSIFRITFGVSLPFNASAPILAFSGTRSTISSPKTSTENEKNQKNMLYVCNHRTLLDPLYISHTINKPLSAVTYSMSRVNEIISPIKTTRLTRDRARDRDSMEKMLCEGNLVVCPEGTTCREPYLLRFSPLFAELTDDIVPVAVDVKVSMFYGTTASGYKCLDPVFHYLNPNPVYFVKILERIPTSQTCQKGGKSPVEVANFVQNEIGKSLGFGCTNLTRKDKYMILAGNEGVSSNNKQSLS*