AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0773480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773480 MtrunA17_Chr5g0427361 93.725 255 14 2 1 255 1 253 2.80e-154 429
Msa0773480 MtrunA17_Chr7g0255961 35.204 196 96 3 26 220 64 229 2.70e-32 118
Msa0773480 MtrunA17_Chr5g0412371 34.914 232 110 7 2 224 1 200 1.59e-30 112
Msa0773480 MtrunA17_Chr1g0198801 35.616 219 104 4 31 245 152 337 4.28e-28 109
Msa0773480 MtrunA17_Chr2g0325521 35.417 192 98 2 30 220 52 218 8.83e-25 99.0
Msa0773480 MtrunA17_Chr3g0113721 30.542 203 100 3 31 223 33 204 2.44e-23 94.7
Msa0773480 MtrunA17_Chr4g0041051 39.634 164 73 2 28 191 60 197 2.05e-21 90.1
Msa0773480 MtrunA17_Chr4g0045901 32.836 201 91 4 30 220 68 234 8.76e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0773480 AT3G25710.1 37.864 206 84 4 33 220 128 307 3.13e-31 118
Msa0773480 AT3G56770.1 36.458 192 89 3 31 220 39 199 6.98e-30 112
Msa0773480 AT1G68810.1 40.761 184 77 3 9 191 152 304 2.99e-29 113
Msa0773480 AT2G41130.1 35.233 193 97 2 28 220 58 222 1.66e-25 101
Msa0773480 AT4G38070.1 35.465 172 78 4 29 199 83 222 1.99e-22 93.2
Msa0773480 AT2G40200.1 31.847 157 79 2 33 186 59 190 7.78e-19 83.6

Find 84 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGAGACATGGCCGTTTC+CGG 0.216545 5_2:-48451724 Msa0773480:CDS
CAACCTCACGAGCCCGTTTC+CGG 0.259442 5_2:+48451370 None:intergenic
AACCCGTAGAATCTTCTTAA+AGG 0.270050 5_2:+48452253 None:intergenic
CGCTAACAACGACGCCTTAT+CGG 0.275455 5_2:+48451869 None:intergenic
GGAGAAGGAGGTTGAAGCTT+TGG 0.288893 5_2:-48451463 Msa0773480:CDS
CGTTTCCGGCCCAACAAAAT+CGG 0.326862 5_2:+48451384 None:intergenic
AAGAAGCATGCAAGAGTCAT+AGG 0.355128 5_2:-48452159 Msa0773480:CDS
ACAAGCAATCCGTTCGGTTC+TGG 0.360599 5_2:-48451586 Msa0773480:CDS
GAAAGCTGTTATAGAGAATA+GGG 0.375468 5_2:-48451430 Msa0773480:CDS
ACTGAGACATGGCCGTTTCC+GGG 0.384662 5_2:-48451723 Msa0773480:CDS
TGAAAGCTGTTATAGAGAAT+AGG 0.402504 5_2:-48451431 Msa0773480:CDS
TCATCACCACCTTCACCTTT+AGG 0.406150 5_2:+48451495 None:intergenic
ATATGTAGTTGTTTAGTAGT+TGG 0.407115 5_2:-48451258 Msa0773480:three_prime_UTR
CCGTTCGGTTCTGGCGAAAC+CGG 0.414266 5_2:-48451577 Msa0773480:CDS
GGAGGTTGAAGCTTTGGAAA+GGG 0.425401 5_2:-48451457 Msa0773480:CDS
GGCGTCGTTGTTAGCGGAAG+TGG 0.431203 5_2:-48451862 Msa0773480:CDS
TGAATGACCTTTGTGGTGTT+AGG 0.434067 5_2:+48452073 None:intergenic
GATTCTACGGGTTTGAGAGC+TGG 0.437215 5_2:-48452243 Msa0773480:CDS
TATTGCGGTGGTAGAGAGGA+AGG 0.446965 5_2:-48451675 Msa0773480:CDS
GACAAAGAGTGTTGTGGTGG+TGG 0.449486 5_2:-48451523 Msa0773480:CDS
GTCATCATCTCAGCTCGAAC+CGG 0.451864 5_2:+48451558 None:intergenic
CCTCCTTTAAGAAGATTCTA+CGG 0.460188 5_2:-48452256 Msa0773480:CDS
TCAAATCAAAGAAAATATCA+TGG 0.461801 5_2:-48451231 Msa0773480:three_prime_UTR
GGATTGCTTGTGTTAGATCA+TGG 0.468042 5_2:+48451598 None:intergenic
GAAGAAGGAAGCTGATGAAA+TGG 0.473728 5_2:-48451820 Msa0773480:CDS
CTCCTTTAAGAAGATTCTAC+GGG 0.475979 5_2:-48452255 Msa0773480:CDS
TGATGATGGAAGAGAGGAGA+AGG 0.477004 5_2:-48451478 Msa0773480:CDS
CTTGTTGTTGAGGAAGAATG+AGG 0.491130 5_2:-48451340 Msa0773480:CDS
ACACGTGAAACGGTCGAAGA+AGG 0.492375 5_2:-48451835 Msa0773480:CDS
GAGGTTGAAGCTTTGGAAAG+GGG 0.493508 5_2:-48451456 Msa0773480:CDS
TGATGGAAGAGAGGAGAAGG+AGG 0.494593 5_2:-48451475 Msa0773480:CDS
TCTTCATGCAGCGGAGAACC+TGG 0.503192 5_2:-48451768 Msa0773480:CDS
CGATAAGGCGTCGTTGTTAG+CGG 0.504065 5_2:-48451868 Msa0773480:CDS
GTTTCCGGCCCAACAAAATC+GGG 0.508277 5_2:+48451385 None:intergenic
AGGAGGTTGAAGCTTTGGAA+AGG 0.510777 5_2:-48451458 Msa0773480:CDS
AAAGGTGAAGGTGGTGATGA+TGG 0.514370 5_2:-48451492 Msa0773480:CDS
TCGTCTGATTCTCCCGGAAA+CGG 0.515198 5_2:+48451711 None:intergenic
ATGGCGAATCGTCGCAATGA+CGG 0.517551 5_2:-48451801 Msa0773480:CDS
AAGTGGTGCAACACGTGAAA+CGG 0.520362 5_2:-48451845 Msa0773480:CDS
AGAAGCATGCAAGAGTCATA+GGG 0.522990 5_2:-48452158 Msa0773480:CDS
GGGCCGGAAACGGGCTCGTG+AGG 0.526347 5_2:-48451373 Msa0773480:CDS
TTGACTGTTCCTTGTTGTTG+AGG 0.530398 5_2:-48451350 Msa0773480:CDS
ACCTGGTTCAGTTAATTCTA+CGG 0.531153 5_2:-48451751 Msa0773480:CDS
GATGGATAATAAAGACTATG+AGG 0.533000 5_2:-48452221 Msa0773480:CDS
GCTGAGATGATGACGGTTGG+AGG 0.537478 5_2:-48451549 Msa0773480:CDS
AGTGTGTTGTGAGGATCGAC+CGG 0.538414 5_2:-48451628 Msa0773480:CDS
GAGTTATTGCGGTGGTAGAG+AGG 0.542449 5_2:-48451679 Msa0773480:CDS
GTCGCCTCGTCTGATTCTCC+CGG 0.551846 5_2:+48451705 None:intergenic
AGAAACAAAGACTACTATGA+AGG 0.553119 5_2:-48451205 Msa0773480:three_prime_UTR
GTGGAGTGGCCTAAAGGTGA+AGG 0.554899 5_2:-48451504 Msa0773480:CDS
ATTGCGGTGGTAGAGAGGAA+GGG 0.561452 5_2:-48451674 Msa0773480:CDS
TTGCGGTGGTAGAGAGGAAG+GGG 0.563178 5_2:-48451673 Msa0773480:CDS
CGAGCTGAGATGATGACGGT+TGG 0.565864 5_2:-48451552 Msa0773480:CDS
AGATGATGACGGTTGGAGGT+AGG 0.573370 5_2:-48451545 Msa0773480:CDS
AGGTTCTCCGCTGCATGAAG+AGG 0.577024 5_2:+48451770 None:intergenic
CTACGGGTTTGAGAGCTGGA+TGG 0.582407 5_2:-48452239 Msa0773480:CDS
GTGGTGGTGGAGTGGCCTAA+AGG 0.584300 5_2:-48451510 Msa0773480:CDS
AGGTGGTGATGATGGAAGAG+AGG 0.588110 5_2:-48451484 Msa0773480:CDS
CTCATTCTTCCTCAACAACA+AGG 0.601988 5_2:+48451341 None:intergenic
CCGTAGAATCTTCTTAAAGG+AGG 0.605275 5_2:+48452256 None:intergenic
TCTAACACAAGCAATCCGTT+CGG 0.605327 5_2:-48451592 Msa0773480:CDS
TGACCTTTGTGGTGTTAGGA+AGG 0.606748 5_2:+48452077 None:intergenic
TCCGTAGAATTAACTGAACC+AGG 0.607098 5_2:+48451750 None:intergenic
AATGGAATGAATGACCTTTG+TGG 0.614221 5_2:+48452066 None:intergenic
GTTAGATCATGGTTCAGACC+CGG 0.622519 5_2:+48451609 None:intergenic
GTGTGTTGTGAGGATCGACC+GGG 0.625955 5_2:-48451627 Msa0773480:CDS
GAGTCTTCCTCTTCATGCAG+CGG 0.627449 5_2:-48451777 Msa0773480:CDS
AGAGTCATAGGGAAGCTGAA+AGG 0.630828 5_2:-48452147 Msa0773480:CDS
CCGGTTTCGCCAGAACCGAA+CGG 0.637182 5_2:+48451577 None:intergenic
GTAGAGAGGAAGGGGAACCA+CGG 0.640152 5_2:-48451665 Msa0773480:CDS
CTACGGAGGTAACTGAGACA+TGG 0.643428 5_2:-48451734 Msa0773480:CDS
GAGTGGCCTAAAGGTGAAGG+TGG 0.645085 5_2:-48451501 Msa0773480:CDS
AGAGTGTTGTGGTGGTGGAG+TGG 0.646685 5_2:-48451518 Msa0773480:CDS
ACTGTCACTTTCATCCTCCG+TGG 0.656210 5_2:+48451648 None:intergenic
TAGGACAAAGAGTGTTGTGG+TGG 0.658865 5_2:-48451526 Msa0773480:CDS
GAGGCGACAGTGAGTTATTG+CGG 0.669787 5_2:-48451690 Msa0773480:CDS
GAGAGGAAGGGGAACCACGG+AGG 0.671020 5_2:-48451662 Msa0773480:CDS
AGGTAGGACAAAGAGTGTTG+TGG 0.684138 5_2:-48451529 Msa0773480:CDS
TGGTTCAGTTAATTCTACGG+AGG 0.696147 5_2:-48451748 Msa0773480:CDS
GGTTCGAGCTGAGATGATGA+CGG 0.696578 5_2:-48451556 Msa0773480:CDS
TCTCCTTCCTAACACCACAA+AGG 0.703951 5_2:-48452080 Msa0773480:intron
GAAAGTGACAGTGTGTTGTG+AGG 0.711096 5_2:-48451637 Msa0773480:CDS
GCGACAGTGAGTTATTGCGG+TGG 0.734204 5_2:-48451687 Msa0773480:CDS
GTTTCCGGGAGAATCAGACG+AGG 0.775354 5_2:-48451709 Msa0773480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTGTTTCTTATAAAAA+TGG + chr5_2:48451524-48451543 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_2:48451570-48451589 Msa0773480:CDS 15.0%
!!! TCATTTGTTTCTTATAAAAA+TGG + chr5_2:48451524-48451543 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_2:48451570-48451589 Msa0773480:CDS 15.0%
!! TCAAATCAAAGAAAATATCA+TGG - chr5_2:48452285-48452304 Msa0773480:five_prime_UTR 20.0%
!! TCAAATCAAAGAAAATATCA+TGG - chr5_2:48452285-48452304 Msa0773480:five_prime_UTR 20.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_2:48451476-48451495 None:intergenic 25.0%
! TCTTATAAAAATGGACATGA+GGG + chr5_2:48451515-48451534 None:intergenic 25.0%
! TTCTTATAAAAATGGACATG+AGG + chr5_2:48451516-48451535 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr5_2:48451545-48451564 Msa0773480:CDS 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_2:48451573-48451592 Msa0773480:CDS 25.0%
! ATATGTAGTTGTTTAGTAGT+TGG - chr5_2:48452258-48452277 Msa0773480:CDS 25.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_2:48451476-48451495 None:intergenic 25.0%
! TCTTATAAAAATGGACATGA+GGG + chr5_2:48451515-48451534 None:intergenic 25.0%
! TTCTTATAAAAATGGACATG+AGG + chr5_2:48451516-48451535 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr5_2:48451545-48451564 Msa0773480:CDS 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_2:48451573-48451592 Msa0773480:CDS 25.0%
! ATATGTAGTTGTTTAGTAGT+TGG - chr5_2:48452258-48452277 Msa0773480:CDS 25.0%
!!! TCTTTTTCTTCTTGGTTCTA+TGG + chr5_2:48451207-48451226 None:intergenic 30.0%
! CTTCTTCTTCTTTTTCTTCT+TGG + chr5_2:48451215-48451234 None:intergenic 30.0%
GATGGATAATAAAGACTATG+AGG - chr5_2:48451295-48451314 Msa0773480:three_prime_UTR 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_2:48451471-48451490 None:intergenic 30.0%
TGAAAGCTGTTATAGAGAAT+AGG - chr5_2:48452085-48452104 Msa0773480:CDS 30.0%
GAAAGCTGTTATAGAGAATA+GGG - chr5_2:48452086-48452105 Msa0773480:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_2:48452199-48452218 Msa0773480:CDS 30.0%
AGAAACAAAGACTACTATGA+AGG - chr5_2:48452311-48452330 Msa0773480:five_prime_UTR 30.0%
!!! TCTTTTTCTTCTTGGTTCTA+TGG + chr5_2:48451207-48451226 None:intergenic 30.0%
! CTTCTTCTTCTTTTTCTTCT+TGG + chr5_2:48451215-48451234 None:intergenic 30.0%
GATGGATAATAAAGACTATG+AGG - chr5_2:48451295-48451314 Msa0773480:three_prime_UTR 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_2:48451471-48451490 None:intergenic 30.0%
TGAAAGCTGTTATAGAGAAT+AGG - chr5_2:48452085-48452104 Msa0773480:CDS 30.0%
GAAAGCTGTTATAGAGAATA+GGG - chr5_2:48452086-48452105 Msa0773480:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_2:48452199-48452218 Msa0773480:CDS 30.0%
AGAAACAAAGACTACTATGA+AGG - chr5_2:48452311-48452330 Msa0773480:five_prime_UTR 30.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_2:48451260-48451279 Msa0773480:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_2:48451261-48451280 Msa0773480:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_2:48451266-48451285 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_2:48451453-48451472 None:intergenic 35.0%
! ATTTTATGTGCAGTCCGATA+AGG - chr5_2:48451633-48451652 Msa0773480:CDS 35.0%
ACCTGGTTCAGTTAATTCTA+CGG - chr5_2:48451765-48451784 Msa0773480:CDS 35.0%
! TATAGAGAATAGGGCTTTTG+TGG - chr5_2:48452095-48452114 Msa0773480:CDS 35.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_2:48451260-48451279 Msa0773480:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_2:48451261-48451280 Msa0773480:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_2:48451266-48451285 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_2:48451453-48451472 None:intergenic 35.0%
! ATTTTATGTGCAGTCCGATA+AGG - chr5_2:48451633-48451652 Msa0773480:CDS 35.0%
ACCTGGTTCAGTTAATTCTA+CGG - chr5_2:48451765-48451784 Msa0773480:CDS 35.0%
! TATAGAGAATAGGGCTTTTG+TGG - chr5_2:48452095-48452114 Msa0773480:CDS 35.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_2:48451263-48451282 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_2:48451357-48451376 Msa0773480:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_2:48451358-48451377 Msa0773480:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_2:48451446-48451465 None:intergenic 40.0%
GAAGAAGGAAGCTGATGAAA+TGG - chr5_2:48451696-48451715 Msa0773480:CDS 40.0%
TCCGTAGAATTAACTGAACC+AGG + chr5_2:48451769-48451788 None:intergenic 40.0%
TGGTTCAGTTAATTCTACGG+AGG - chr5_2:48451768-48451787 Msa0773480:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_2:48451921-48451940 None:intergenic 40.0%
TCTAACACAAGCAATCCGTT+CGG - chr5_2:48451924-48451943 Msa0773480:intron 40.0%
! AATAGGGCTTTTGTGGATTC+TGG - chr5_2:48452102-48452121 Msa0773480:CDS 40.0%
! ATAGGGCTTTTGTGGATTCT+GGG - chr5_2:48452103-48452122 Msa0773480:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_2:48452166-48452185 Msa0773480:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_2:48452178-48452197 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_2:48452176-48452195 Msa0773480:CDS 40.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_2:48451263-48451282 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_2:48451357-48451376 Msa0773480:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_2:48451358-48451377 Msa0773480:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_2:48451446-48451465 None:intergenic 40.0%
GAAGAAGGAAGCTGATGAAA+TGG - chr5_2:48451696-48451715 Msa0773480:CDS 40.0%
TCCGTAGAATTAACTGAACC+AGG + chr5_2:48451769-48451788 None:intergenic 40.0%
TGGTTCAGTTAATTCTACGG+AGG - chr5_2:48451768-48451787 Msa0773480:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_2:48451921-48451940 None:intergenic 40.0%
TCTAACACAAGCAATCCGTT+CGG - chr5_2:48451924-48451943 Msa0773480:intron 40.0%
! AATAGGGCTTTTGTGGATTC+TGG - chr5_2:48452102-48452121 Msa0773480:CDS 40.0%
! ATAGGGCTTTTGTGGATTCT+GGG - chr5_2:48452103-48452122 Msa0773480:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_2:48452166-48452185 Msa0773480:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_2:48452178-48452197 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_2:48452176-48452195 Msa0773480:CDS 40.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_2:48451369-48451388 Msa0773480:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_2:48451436-48451455 Msa0773480:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_2:48451442-48451461 None:intergenic 45.0%
!! TTTAGTGGTGGCTCTGAACA+CGG - chr5_2:48451585-48451604 Msa0773480:CDS 45.0%
AAGTGGTGCAACACGTGAAA+CGG - chr5_2:48451671-48451690 Msa0773480:CDS 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_2:48451879-48451898 Msa0773480:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_2:48451910-48451929 None:intergenic 45.0%
!! AGGTAGGACAAAGAGTGTTG+TGG - chr5_2:48451987-48452006 Msa0773480:intron 45.0%
!! TAGGACAAAGAGTGTTGTGG+TGG - chr5_2:48451990-48452009 Msa0773480:intron 45.0%
TCATCACCACCTTCACCTTT+AGG + chr5_2:48452024-48452043 None:intergenic 45.0%
! AAAGGTGAAGGTGGTGATGA+TGG - chr5_2:48452024-48452043 Msa0773480:intron 45.0%
!! TGATGATGGAAGAGAGGAGA+AGG - chr5_2:48452038-48452057 Msa0773480:intron 45.0%
!! AGGAGGTTGAAGCTTTGGAA+AGG - chr5_2:48452058-48452077 Msa0773480:intron 45.0%
!! GGAGGTTGAAGCTTTGGAAA+GGG - chr5_2:48452059-48452078 Msa0773480:intron 45.0%
!! GAGGTTGAAGCTTTGGAAAG+GGG - chr5_2:48452060-48452079 Msa0773480:intron 45.0%
! GCTTTTGTGGATTCTGGGTT+GGG - chr5_2:48452108-48452127 Msa0773480:CDS 45.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_2:48451369-48451388 Msa0773480:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_2:48451436-48451455 Msa0773480:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_2:48451442-48451461 None:intergenic 45.0%
!! TTTAGTGGTGGCTCTGAACA+CGG - chr5_2:48451585-48451604 Msa0773480:CDS 45.0%
AAGTGGTGCAACACGTGAAA+CGG - chr5_2:48451671-48451690 Msa0773480:CDS 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_2:48451879-48451898 Msa0773480:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_2:48451910-48451929 None:intergenic 45.0%
!! AGGTAGGACAAAGAGTGTTG+TGG - chr5_2:48451987-48452006 Msa0773480:intron 45.0%
!! TAGGACAAAGAGTGTTGTGG+TGG - chr5_2:48451990-48452009 Msa0773480:intron 45.0%
TCATCACCACCTTCACCTTT+AGG + chr5_2:48452024-48452043 None:intergenic 45.0%
! AAAGGTGAAGGTGGTGATGA+TGG - chr5_2:48452024-48452043 Msa0773480:intron 45.0%
!! TGATGATGGAAGAGAGGAGA+AGG - chr5_2:48452038-48452057 Msa0773480:intron 45.0%
!! AGGAGGTTGAAGCTTTGGAA+AGG - chr5_2:48452058-48452077 Msa0773480:intron 45.0%
!! GGAGGTTGAAGCTTTGGAAA+GGG - chr5_2:48452059-48452078 Msa0773480:intron 45.0%
!! GAGGTTGAAGCTTTGGAAAG+GGG - chr5_2:48452060-48452079 Msa0773480:intron 45.0%
! GCTTTTGTGGATTCTGGGTT+GGG - chr5_2:48452108-48452127 Msa0773480:CDS 45.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_2:48451273-48451292 Msa0773480:three_prime_UTR 50.0%
CGCTAACAACGACGCCTTAT+CGG + chr5_2:48451650-48451669 None:intergenic 50.0%
!! CGATAAGGCGTCGTTGTTAG+CGG - chr5_2:48451648-48451667 Msa0773480:CDS 50.0%
ACACGTGAAACGGTCGAAGA+AGG - chr5_2:48451681-48451700 Msa0773480:CDS 50.0%
ATGGCGAATCGTCGCAATGA+CGG - chr5_2:48451715-48451734 Msa0773480:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_2:48451739-48451758 Msa0773480:CDS 50.0%
CTACGGAGGTAACTGAGACA+TGG - chr5_2:48451782-48451801 Msa0773480:CDS 50.0%
AACTGAGACATGGCCGTTTC+CGG - chr5_2:48451792-48451811 Msa0773480:CDS 50.0%
TCGTCTGATTCTCCCGGAAA+CGG + chr5_2:48451808-48451827 None:intergenic 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_2:48451826-48451845 Msa0773480:CDS 50.0%
GAGTTATTGCGGTGGTAGAG+AGG - chr5_2:48451837-48451856 Msa0773480:CDS 50.0%
TATTGCGGTGGTAGAGAGGA+AGG - chr5_2:48451841-48451860 Msa0773480:CDS 50.0%
ATTGCGGTGGTAGAGAGGAA+GGG - chr5_2:48451842-48451861 Msa0773480:CDS 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_2:48451871-48451890 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_2:48451888-48451907 Msa0773480:intron 50.0%
ACAAGCAATCCGTTCGGTTC+TGG - chr5_2:48451930-48451949 Msa0773480:intron 50.0%
GTCATCATCTCAGCTCGAAC+CGG + chr5_2:48451961-48451980 None:intergenic 50.0%
GGTTCGAGCTGAGATGATGA+CGG - chr5_2:48451960-48451979 Msa0773480:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_2:48451971-48451990 Msa0773480:intron 50.0%
!! GACAAAGAGTGTTGTGGTGG+TGG - chr5_2:48451993-48452012 Msa0773480:intron 50.0%
!! AGGTGGTGATGATGGAAGAG+AGG - chr5_2:48452032-48452051 Msa0773480:intron 50.0%
TGATGGAAGAGAGGAGAAGG+AGG - chr5_2:48452041-48452060 Msa0773480:intron 50.0%
GGAGAAGGAGGTTGAAGCTT+TGG - chr5_2:48452053-48452072 Msa0773480:intron 50.0%
! GGCTTTTGTGGATTCTGGGT+TGG - chr5_2:48452107-48452126 Msa0773480:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_2:48452134-48452153 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_2:48452135-48452154 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_2:48452133-48452152 Msa0773480:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_2:48452134-48452153 Msa0773480:CDS 50.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_2:48451273-48451292 Msa0773480:three_prime_UTR 50.0%
CGCTAACAACGACGCCTTAT+CGG + chr5_2:48451650-48451669 None:intergenic 50.0%
!! CGATAAGGCGTCGTTGTTAG+CGG - chr5_2:48451648-48451667 Msa0773480:CDS 50.0%
ACACGTGAAACGGTCGAAGA+AGG - chr5_2:48451681-48451700 Msa0773480:CDS 50.0%
ATGGCGAATCGTCGCAATGA+CGG - chr5_2:48451715-48451734 Msa0773480:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_2:48451739-48451758 Msa0773480:CDS 50.0%
CTACGGAGGTAACTGAGACA+TGG - chr5_2:48451782-48451801 Msa0773480:CDS 50.0%
AACTGAGACATGGCCGTTTC+CGG - chr5_2:48451792-48451811 Msa0773480:CDS 50.0%
TCGTCTGATTCTCCCGGAAA+CGG + chr5_2:48451808-48451827 None:intergenic 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_2:48451826-48451845 Msa0773480:CDS 50.0%
GAGTTATTGCGGTGGTAGAG+AGG - chr5_2:48451837-48451856 Msa0773480:CDS 50.0%
TATTGCGGTGGTAGAGAGGA+AGG - chr5_2:48451841-48451860 Msa0773480:CDS 50.0%
ATTGCGGTGGTAGAGAGGAA+GGG - chr5_2:48451842-48451861 Msa0773480:CDS 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_2:48451871-48451890 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_2:48451888-48451907 Msa0773480:intron 50.0%
ACAAGCAATCCGTTCGGTTC+TGG - chr5_2:48451930-48451949 Msa0773480:intron 50.0%
GTCATCATCTCAGCTCGAAC+CGG + chr5_2:48451961-48451980 None:intergenic 50.0%
GGTTCGAGCTGAGATGATGA+CGG - chr5_2:48451960-48451979 Msa0773480:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_2:48451971-48451990 Msa0773480:intron 50.0%
!! GACAAAGAGTGTTGTGGTGG+TGG - chr5_2:48451993-48452012 Msa0773480:intron 50.0%
!! AGGTGGTGATGATGGAAGAG+AGG - chr5_2:48452032-48452051 Msa0773480:intron 50.0%
TGATGGAAGAGAGGAGAAGG+AGG - chr5_2:48452041-48452060 Msa0773480:intron 50.0%
GGAGAAGGAGGTTGAAGCTT+TGG - chr5_2:48452053-48452072 Msa0773480:intron 50.0%
! GGCTTTTGTGGATTCTGGGT+TGG - chr5_2:48452107-48452126 Msa0773480:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_2:48452134-48452153 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_2:48452135-48452154 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_2:48452133-48452152 Msa0773480:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_2:48452134-48452153 Msa0773480:CDS 50.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_2:48451277-48451296 Msa0773480:three_prime_UTR 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_2:48451749-48451768 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_2:48451748-48451767 Msa0773480:CDS 55.0%
ACTGAGACATGGCCGTTTCC+GGG - chr5_2:48451793-48451812 Msa0773480:CDS 55.0%
GTTTCCGGGAGAATCAGACG+AGG - chr5_2:48451807-48451826 Msa0773480:CDS 55.0%
GCGACAGTGAGTTATTGCGG+TGG - chr5_2:48451829-48451848 Msa0773480:CDS 55.0%
TTGCGGTGGTAGAGAGGAAG+GGG - chr5_2:48451843-48451862 Msa0773480:CDS 55.0%
GTAGAGAGGAAGGGGAACCA+CGG - chr5_2:48451851-48451870 Msa0773480:CDS 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_2:48451889-48451908 Msa0773480:intron 55.0%
!! CGAGCTGAGATGATGACGGT+TGG - chr5_2:48451964-48451983 Msa0773480:intron 55.0%
!! GCTGAGATGATGACGGTTGG+AGG - chr5_2:48451967-48451986 Msa0773480:intron 55.0%
!! AGAGTGTTGTGGTGGTGGAG+TGG - chr5_2:48451998-48452017 Msa0773480:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_2:48452012-48452031 Msa0773480:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_2:48452015-48452034 Msa0773480:intron 55.0%
!! TGGGTTGGGCCCGATTTTGT+TGG - chr5_2:48452122-48452141 Msa0773480:CDS 55.0%
! GGGTTGGGCCCGATTTTGTT+GGG - chr5_2:48452123-48452142 Msa0773480:CDS 55.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_2:48451277-48451296 Msa0773480:three_prime_UTR 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_2:48451749-48451768 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_2:48451748-48451767 Msa0773480:CDS 55.0%
ACTGAGACATGGCCGTTTCC+GGG - chr5_2:48451793-48451812 Msa0773480:CDS 55.0%
GTTTCCGGGAGAATCAGACG+AGG - chr5_2:48451807-48451826 Msa0773480:CDS 55.0%
GCGACAGTGAGTTATTGCGG+TGG - chr5_2:48451829-48451848 Msa0773480:CDS 55.0%
TTGCGGTGGTAGAGAGGAAG+GGG - chr5_2:48451843-48451862 Msa0773480:CDS 55.0%
GTAGAGAGGAAGGGGAACCA+CGG - chr5_2:48451851-48451870 Msa0773480:CDS 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_2:48451889-48451908 Msa0773480:intron 55.0%
!! CGAGCTGAGATGATGACGGT+TGG - chr5_2:48451964-48451983 Msa0773480:intron 55.0%
!! GCTGAGATGATGACGGTTGG+AGG - chr5_2:48451967-48451986 Msa0773480:intron 55.0%
!! AGAGTGTTGTGGTGGTGGAG+TGG - chr5_2:48451998-48452017 Msa0773480:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_2:48452012-48452031 Msa0773480:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_2:48452015-48452034 Msa0773480:intron 55.0%
!! TGGGTTGGGCCCGATTTTGT+TGG - chr5_2:48452122-48452141 Msa0773480:CDS 55.0%
! GGGTTGGGCCCGATTTTGTT+GGG - chr5_2:48452123-48452142 Msa0773480:CDS 55.0%
GGCGTCGTTGTTAGCGGAAG+TGG - chr5_2:48451654-48451673 Msa0773480:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+CGG + chr5_2:48451814-48451833 None:intergenic 60.0%
CCGGTTTCGCCAGAACCGAA+CGG + chr5_2:48451942-48451961 None:intergenic 60.0%
! CCGTTCGGTTCTGGCGAAAC+CGG - chr5_2:48451939-48451958 Msa0773480:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_2:48452006-48452025 Msa0773480:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_2:48452127-48452146 Msa0773480:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_2:48452149-48452168 None:intergenic 60.0%
GGCGTCGTTGTTAGCGGAAG+TGG - chr5_2:48451654-48451673 Msa0773480:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+CGG + chr5_2:48451814-48451833 None:intergenic 60.0%
CCGGTTTCGCCAGAACCGAA+CGG + chr5_2:48451942-48451961 None:intergenic 60.0%
! CCGTTCGGTTCTGGCGAAAC+CGG - chr5_2:48451939-48451958 Msa0773480:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_2:48452006-48452025 Msa0773480:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_2:48452127-48452146 Msa0773480:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_2:48452149-48452168 None:intergenic 60.0%
GAGAGGAAGGGGAACCACGG+AGG - chr5_2:48451854-48451873 Msa0773480:CDS 65.0%
GAGAGGAAGGGGAACCACGG+AGG - chr5_2:48451854-48451873 Msa0773480:CDS 65.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_2:48452143-48452162 Msa0773480:CDS 75.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_2:48452143-48452162 Msa0773480:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 48451158 48452380 48451158 ID=Msa0773480;Name=Msa0773480
chr5_2 mRNA 48451158 48452380 48451158 ID=Msa0773480-mRNA-1;Parent=Msa0773480;Name=Msa0773480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|171|255
chr5_2 exon 48451158 48451892 48451158 ID=Msa0773480-mRNA-1:exon:14482;Parent=Msa0773480-mRNA-1
chr5_2 exon 48452081 48452380 48452081 ID=Msa0773480-mRNA-1:exon:14481;Parent=Msa0773480-mRNA-1
chr5_2 five_prime_UTR 48452285 48452380 48452285 ID=Msa0773480-mRNA-1:five_prime_utr;Parent=Msa0773480-mRNA-1
chr5_2 CDS 48452081 48452284 48452081 ID=Msa0773480-mRNA-1:cds;Parent=Msa0773480-mRNA-1
chr5_2 CDS 48451329 48451892 48451329 ID=Msa0773480-mRNA-1:cds;Parent=Msa0773480-mRNA-1
chr5_2 three_prime_UTR 48451158 48451328 48451158 ID=Msa0773480-mRNA-1:three_prime_utr;Parent=Msa0773480-mRNA-1
Gene Sequence

>Msa0773480

ATGCTTCCTCCTTTAAGAAGATTCTACGGGTTTGAGAGCTGGATGGATAATAAAGACTATGAGGAAATCAACTGTGACAATTCAAAATCTGAGAGAAAATCAAAAGAAGCATGCAAGAGTCATAGGGAAGCTGAAAGGAGACGCAGACAGAGAATCAACGCTCATCTCAACACTCTTCGCTCTCTCCTTCCTAACACCACAAAGTCCGATAAGGCGTCGTTGTTAGCGGAAGTGGTGCAACACGTGAAACGGTCGAAGAAGGAAGCTGATGAAATGGCGAATCGTCGCAATGACGGAGAGTCTTCCTCTTCATGCAGCGGAGAACCTGGTTCAGTTAATTCTACGGAGGTAACTGAGACATGGCCGTTTCCGGGAGAATCAGACGAGGCGACAGTGAGTTATTGCGGTGGTAGAGAGGAAGGGGAACCACGGAGGATGAAAGTGACAGTGTGTTGTGAGGATCGACCGGGTCTGAACCATGATCTAACACAAGCAATCCGTTCGGTTCTGGCGAAACCGGTTCGAGCTGAGATGATGACGGTTGGAGGTAGGACAAAGAGTGTTGTGGTGGTGGAGTGGCCTAAAGGTGAAGGTGGTGATGATGGAAGAGAGGAGAAGGAGGTTGAAGCTTTGGAAAGGGGTTTGAAAGCTGTTATAGAGAATAGGGCTTTTGTGGATTCTGGGTTGGGCCCGATTTTGTTGGGCCGGAAACGGGCTCGTGAGGTTGACTGTTCCTTGTTGTTGAGGAAGAATGAGGACTTTTGCTAA
Protein sequence

>Msa0773480

MLPPLRRFYGFESWMDNKDYEEINCDNSKSERKSKEACKSHREAERRRRQRINAHLNTLRSLLPNTTKSDKASLLAEVVQHVKRSKKEADEMANRRNDGESSSSCSGEPGSVNSTEVTETWPFPGESDEATVSYCGGREEGEPRRMKVTVCCEDRPGLNHDLTQAIRSVLAKPVRAEMMTVGGRTKSVVVVEWPKGEGGDDGREEKEVEALERGLKAVIENRAFVDSGLGPILLGRKRAREVDCSLLLRKNEDFC*