AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0851270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0851270 MtrunA17_Chr5g0427361 94.488 254 13 1 1 254 1 253 7.62e-157 436
Msa0851270 MtrunA17_Chr5g0412371 33.766 231 113 5 2 223 1 200 2.03e-33 120
Msa0851270 MtrunA17_Chr7g0255961 39.766 171 76 3 26 194 64 209 8.42e-32 117
Msa0851270 MtrunA17_Chr1g0198801 37.900 219 98 5 31 244 152 337 2.73e-30 115
Msa0851270 MtrunA17_Chr2g0325521 41.358 162 69 2 30 190 52 188 1.98e-25 100
Msa0851270 MtrunA17_Chr3g0113721 31.527 203 97 3 31 222 33 204 5.38e-25 99.0
Msa0851270 MtrunA17_Chr4g0041051 40.244 164 71 3 28 190 60 197 6.35e-21 88.6
Msa0851270 MtrunA17_Chr4g0045901 34.500 200 88 4 30 219 68 234 2.16e-17 79.3
Msa0851270 MtrunA17_Chr7g0231461 40.741 81 45 2 19 97 284 363 2.03e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0851270 AT3G56770.1 38.144 194 82 5 31 219 39 199 3.08e-31 115
Msa0851270 AT3G25710.1 38.537 205 83 4 33 219 128 307 5.18e-31 117
Msa0851270 AT1G68810.1 42.391 184 73 4 9 190 152 304 2.61e-30 116
Msa0851270 AT2G41130.1 35.233 193 96 3 28 219 58 222 3.43e-25 100
Msa0851270 AT4G38070.1 36.842 171 76 4 29 198 83 222 1.42e-23 96.3
Msa0851270 AT2G40200.1 29.508 183 102 3 33 215 59 214 2.96e-20 87.4

Find 81 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCTCACGAGCCCGTTTC+CGG 0.259442 5_4:+41378607 None:intergenic
AACCCGTAGAATCTTCTTAA+AGG 0.270050 5_4:+41379487 None:intergenic
ACCTGGTTCGGTTAATTCTA+TGG 0.293857 5_4:-41378985 Msa0851270:CDS
GGAGAAGGAGGTAGAAGCTT+TGG 0.321763 5_4:-41378700 Msa0851270:CDS
CGTTTCCGGCCCAACAAAAT+CGG 0.326862 5_4:+41378621 None:intergenic
AAGAAGCATGCAAGAGTCAT+AGG 0.355128 5_4:-41379393 Msa0851270:CDS
GCTGAGACATGGCCGTTTCC+AGG 0.358022 5_4:-41378957 Msa0851270:CDS
ACAAGCAATCCGTTCGGTTC+TGG 0.360599 5_4:-41378823 Msa0851270:CDS
AAAGGTGAAGGTGGTGATAA+TGG 0.377501 5_4:-41378729 Msa0851270:CDS
TTATCACCACCTTCACCTTT+AGG 0.394483 5_4:+41378732 None:intergenic
AGCTTTGGAAAGGGGTTTGA+AGG 0.397072 5_4:-41378685 Msa0851270:CDS
CCGTTCGGTTCTGGCGAAAC+CGG 0.414266 5_4:-41378814 Msa0851270:CDS
GGAGGTAGAAGCTTTGGAAA+GGG 0.416970 5_4:-41378694 Msa0851270:CDS
TGAAGGCTGTTATAGAGAAT+CGG 0.425996 5_4:-41378668 Msa0851270:CDS
TGAATGACCTTTGTGGTGTT+AGG 0.434067 5_4:+41379307 None:intergenic
GATTCTACGGGTTTGAGAGC+TGG 0.437215 5_4:-41379477 Msa0851270:CDS
CAACTGTGACAATTCAAAAT+CGG 0.443233 5_4:-41379428 Msa0851270:CDS
GAAGGCTGTTATAGAGAATC+GGG 0.450367 5_4:-41378667 Msa0851270:CDS
GTCATCATCTCCGCTCGAAC+CGG 0.455212 5_4:+41378795 None:intergenic
TCAAATCAAAGAAAAGATCA+TGG 0.457608 5_4:-41378468 Msa0851270:three_prime_UTR
CCTCCTTTAAGAAGATTCTA+CGG 0.460188 5_4:-41379490 Msa0851270:CDS
GAAGAAGGAAGCTGATGAAA+TGG 0.465712 5_4:-41379054 Msa0851270:CDS
GAGGTAGAAGCTTTGGAAAG+GGG 0.467743 5_4:-41378693 Msa0851270:CDS
GGATTGCTTGTGTTAGATCA+TGG 0.468042 5_4:+41378835 None:intergenic
GTCGCCTCGTCTGATTCTCC+TGG 0.474032 5_4:+41378939 None:intergenic
CTCCTTTAAGAAGATTCTAC+GGG 0.475979 5_4:-41379489 Msa0851270:CDS
TAATGGAAGAGAGGAGAAGG+AGG 0.476276 5_4:-41378712 Msa0851270:CDS
CTTGTTGTTGAGGAAGAATG+AGG 0.491130 5_4:-41378577 Msa0851270:CDS
TCTTCATGCAGCGGAGAACC+TGG 0.503192 5_4:-41379002 Msa0851270:CDS
GTTTCCGGCCCAACAAAATC+GGG 0.508277 5_4:+41378622 None:intergenic
AAGTGGTGCAGCACGTGAAA+CGG 0.509534 5_4:-41379079 Msa0851270:CDS
AGGAGGTAGAAGCTTTGGAA+AGG 0.513380 5_4:-41378695 Msa0851270:CDS
AGTTATTGCGGCAGAGAGGA+AGG 0.514601 5_4:-41378912 Msa0851270:CDS
TGATAATGGAAGAGAGGAGA+AGG 0.516421 5_4:-41378715 Msa0851270:CDS
ATGGCGAATCGTCACAATGA+TGG 0.517077 5_4:-41379035 Msa0851270:CDS
AGAAGCATGCAAGAGTCATA+GGG 0.522990 5_4:-41379392 Msa0851270:CDS
GGCGTCGTTGTTAGCAGAAG+TGG 0.524260 5_4:-41379096 Msa0851270:CDS
GACAAAGAATGTTGTGGTGG+TGG 0.525798 5_4:-41378760 Msa0851270:CDS
GGGCCGGAAACGGGCTCGTG+AGG 0.526347 5_4:-41378610 Msa0851270:CDS
TTGACTGTTCCTTGTTGTTG+AGG 0.530398 5_4:-41378587 Msa0851270:CDS
GATGGATAATAAAGACTATG+AGG 0.533000 5_4:-41379455 Msa0851270:CDS
AGTGTGTTGTGAGGATCGAC+CGG 0.538414 5_4:-41378865 Msa0851270:CDS
ATGCAGCGGAGAACCTGGTT+CGG 0.543278 5_4:-41378997 Msa0851270:CDS
AGGTGGTGATAATGGAAGAG+AGG 0.544625 5_4:-41378721 Msa0851270:CDS
GCGGAGATGATGACGGTTGG+AGG 0.548019 5_4:-41378786 Msa0851270:CDS
AGAAACAAAGACTACTATGA+TGG 0.548632 5_4:-41378442 Msa0851270:three_prime_UTR
GTGGAGTGGCCTAAAGGTGA+AGG 0.554899 5_4:-41378741 Msa0851270:CDS
TCGTCTGATTCTCCTGGAAA+CGG 0.558339 5_4:+41378945 None:intergenic
GCACGTGAAACGGTTGAAGA+AGG 0.561695 5_4:-41379069 Msa0851270:CDS
AGATGATGACGGTTGGAGGT+AGG 0.573370 5_4:-41378782 Msa0851270:CDS
AGGTTCTCCGCTGCATGAAG+AGG 0.577024 5_4:+41379004 None:intergenic
CTACGGGTTTGAGAGCTGGA+TGG 0.582407 5_4:-41379473 Msa0851270:CDS
GTGGTGGTGGAGTGGCCTAA+AGG 0.584300 5_4:-41378747 Msa0851270:CDS
TCCATAGAATTAACCGAACC+AGG 0.589609 5_4:+41378984 None:intergenic
CGAGCGGAGATGATGACGGT+TGG 0.595891 5_4:-41378789 Msa0851270:CDS
CTCATTCTTCCTCAACAACA+AGG 0.601988 5_4:+41378578 None:intergenic
CCGTAGAATCTTCTTAAAGG+AGG 0.605275 5_4:+41379490 None:intergenic
TCTAACACAAGCAATCCGTT+CGG 0.605327 5_4:-41378829 Msa0851270:CDS
TGACCTTTGTGGTGTTAGGA+AGG 0.606748 5_4:+41379311 None:intergenic
CTATGGAGGTAGCTGAGACA+TGG 0.609137 5_4:-41378968 Msa0851270:CDS
AATGGAATGAATGACCTTTG+TGG 0.614221 5_4:+41379300 None:intergenic
TCTGGCGAAACCGGTTCGAG+CGG 0.618429 5_4:-41378805 Msa0851270:CDS
GTTAGATCATGGTTCAGACC+CGG 0.622519 5_4:+41378846 None:intergenic
GTGTGTTGTGAGGATCGACC+GGG 0.625955 5_4:-41378864 Msa0851270:CDS
GAGTCTTCCTCTTCATGCAG+CGG 0.627449 5_4:-41379011 Msa0851270:CDS
AGAGTCATAGGGAAGCTGAA+AGG 0.630828 5_4:-41379381 Msa0851270:CDS
CCGGTTTCGCCAGAACCGAA+CGG 0.637182 5_4:+41378814 None:intergenic
GAGTGGCCTAAAGGTGAAGG+TGG 0.645085 5_4:-41378738 Msa0851270:CDS
GCAGAGAGGAAGGAGAACCA+CGG 0.645622 5_4:-41378902 Msa0851270:CDS
ACTGTCACTTTCATCCTCCG+TGG 0.656210 5_4:+41378885 None:intergenic
GGTTCGAGCGGAGATGATGA+CGG 0.656508 5_4:-41378793 Msa0851270:CDS
GAGGCGACAGTGAGTTATTG+CGG 0.657187 5_4:-41378924 Msa0851270:CDS
TGGTTCGGTTAATTCTATGG+AGG 0.666132 5_4:-41378982 Msa0851270:CDS
AGAATGTTGTGGTGGTGGAG+TGG 0.667583 5_4:-41378755 Msa0851270:CDS
AGTGAGTTATTGCGGCAGAG+AGG 0.669057 5_4:-41378916 Msa0851270:CDS
TAGGACAAAGAATGTTGTGG+TGG 0.689587 5_4:-41378763 Msa0851270:CDS
TCTCCTTCCTAACACCACAA+AGG 0.703951 5_4:-41379314 Msa0851270:intron
AGGTAGGACAAAGAATGTTG+TGG 0.710488 5_4:-41378766 Msa0851270:CDS
GAAAGTGACAGTGTGTTGTG+AGG 0.711096 5_4:-41378874 Msa0851270:CDS
GAGAGGAAGGAGAACCACGG+AGG 0.734471 5_4:-41378899 Msa0851270:CDS
GTTTCCAGGAGAATCAGACG+AGG 0.774251 5_4:-41378943 Msa0851270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTGTTTCTTATAAAAA+AGG + chr5_4:41378787-41378806 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_4:41378833-41378852 Msa0851270:CDS 15.0%
!!! TCATTTGTTTCTTATAAAAA+AGG + chr5_4:41378787-41378806 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_4:41378833-41378852 Msa0851270:CDS 15.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_4:41378739-41378758 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr5_4:41378808-41378827 Msa0851270:CDS 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_4:41378836-41378855 Msa0851270:CDS 25.0%
! TCAAATCAAAGAAAAGATCA+TGG - chr5_4:41379545-41379564 Msa0851270:five_prime_UTR 25.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_4:41378739-41378758 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr5_4:41378808-41378827 Msa0851270:CDS 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_4:41378836-41378855 Msa0851270:CDS 25.0%
! TCAAATCAAAGAAAAGATCA+TGG - chr5_4:41379545-41379564 Msa0851270:five_prime_UTR 25.0%
!!! TTCTTTTCTTCTTGGTTCTA+TGG + chr5_4:41378477-41378496 None:intergenic 30.0%
! TCTTCTTCTTCTTTTCTTCT+TGG + chr5_4:41378485-41378504 None:intergenic 30.0%
GATGGATAATAAAGACTATG+AGG - chr5_4:41378558-41378577 Msa0851270:exon 30.0%
CAACTGTGACAATTCAAAAT+CGG - chr5_4:41378585-41378604 Msa0851270:CDS 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_4:41378734-41378753 None:intergenic 30.0%
TCTTATAAAAAAGGACCTGA+GGG + chr5_4:41378778-41378797 None:intergenic 30.0%
TTCTTATAAAAAAGGACCTG+AGG + chr5_4:41378779-41378798 None:intergenic 30.0%
! ATTTTATGTGCAGTCAGATA+AGG - chr5_4:41378896-41378915 Msa0851270:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_4:41379459-41379478 Msa0851270:CDS 30.0%
AGAAACAAAGACTACTATGA+TGG - chr5_4:41379571-41379590 Msa0851270:five_prime_UTR 30.0%
!!! TTCTTTTCTTCTTGGTTCTA+TGG + chr5_4:41378477-41378496 None:intergenic 30.0%
! TCTTCTTCTTCTTTTCTTCT+TGG + chr5_4:41378485-41378504 None:intergenic 30.0%
GATGGATAATAAAGACTATG+AGG - chr5_4:41378558-41378577 Msa0851270:exon 30.0%
CAACTGTGACAATTCAAAAT+CGG - chr5_4:41378585-41378604 Msa0851270:CDS 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_4:41378734-41378753 None:intergenic 30.0%
TCTTATAAAAAAGGACCTGA+GGG + chr5_4:41378778-41378797 None:intergenic 30.0%
TTCTTATAAAAAAGGACCTG+AGG + chr5_4:41378779-41378798 None:intergenic 30.0%
! ATTTTATGTGCAGTCAGATA+AGG - chr5_4:41378896-41378915 Msa0851270:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_4:41379459-41379478 Msa0851270:CDS 30.0%
AGAAACAAAGACTACTATGA+TGG - chr5_4:41379571-41379590 Msa0851270:five_prime_UTR 30.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_4:41378523-41378542 Msa0851270:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_4:41378524-41378543 Msa0851270:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_4:41378529-41378548 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_4:41378716-41378735 None:intergenic 35.0%
TGAAGGCTGTTATAGAGAAT+CGG - chr5_4:41379345-41379364 Msa0851270:CDS 35.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_4:41378523-41378542 Msa0851270:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_4:41378524-41378543 Msa0851270:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_4:41378529-41378548 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_4:41378716-41378735 None:intergenic 35.0%
TGAAGGCTGTTATAGAGAAT+CGG - chr5_4:41379345-41379364 Msa0851270:CDS 35.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_4:41378526-41378545 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_4:41378620-41378639 Msa0851270:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_4:41378621-41378640 Msa0851270:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_4:41378709-41378728 None:intergenic 40.0%
TGCAAAAATACTAGTCCCTC+AGG - chr5_4:41378760-41378779 Msa0851270:CDS 40.0%
TTTAGTGGTGGCTCTTAACA+CGG - chr5_4:41378848-41378867 Msa0851270:CDS 40.0%
GAAGAAGGAAGCTGATGAAA+TGG - chr5_4:41378959-41378978 Msa0851270:CDS 40.0%
!! ACCTGGTTCGGTTAATTCTA+TGG - chr5_4:41379028-41379047 Msa0851270:CDS 40.0%
TCCATAGAATTAACCGAACC+AGG + chr5_4:41379032-41379051 None:intergenic 40.0%
!! TGGTTCGGTTAATTCTATGG+AGG - chr5_4:41379031-41379050 Msa0851270:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_4:41379181-41379200 None:intergenic 40.0%
TCTAACACAAGCAATCCGTT+CGG - chr5_4:41379184-41379203 Msa0851270:intron 40.0%
AGGTAGGACAAAGAATGTTG+TGG - chr5_4:41379247-41379266 Msa0851270:intron 40.0%
TAGGACAAAGAATGTTGTGG+TGG - chr5_4:41379250-41379269 Msa0851270:intron 40.0%
TTATCACCACCTTCACCTTT+AGG + chr5_4:41379284-41379303 None:intergenic 40.0%
AAAGGTGAAGGTGGTGATAA+TGG - chr5_4:41379284-41379303 Msa0851270:intron 40.0%
!! TGATAATGGAAGAGAGGAGA+AGG - chr5_4:41379298-41379317 Msa0851270:intron 40.0%
GAAGGCTGTTATAGAGAATC+GGG - chr5_4:41379346-41379365 Msa0851270:CDS 40.0%
! TATAGAGAATCGGGCTTTTG+TGG - chr5_4:41379355-41379374 Msa0851270:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_4:41379426-41379445 Msa0851270:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_4:41379438-41379457 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_4:41379436-41379455 Msa0851270:CDS 40.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_4:41378526-41378545 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_4:41378620-41378639 Msa0851270:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_4:41378621-41378640 Msa0851270:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_4:41378709-41378728 None:intergenic 40.0%
TGCAAAAATACTAGTCCCTC+AGG - chr5_4:41378760-41378779 Msa0851270:CDS 40.0%
TTTAGTGGTGGCTCTTAACA+CGG - chr5_4:41378848-41378867 Msa0851270:CDS 40.0%
GAAGAAGGAAGCTGATGAAA+TGG - chr5_4:41378959-41378978 Msa0851270:CDS 40.0%
!! ACCTGGTTCGGTTAATTCTA+TGG - chr5_4:41379028-41379047 Msa0851270:CDS 40.0%
TCCATAGAATTAACCGAACC+AGG + chr5_4:41379032-41379051 None:intergenic 40.0%
!! TGGTTCGGTTAATTCTATGG+AGG - chr5_4:41379031-41379050 Msa0851270:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_4:41379181-41379200 None:intergenic 40.0%
TCTAACACAAGCAATCCGTT+CGG - chr5_4:41379184-41379203 Msa0851270:intron 40.0%
AGGTAGGACAAAGAATGTTG+TGG - chr5_4:41379247-41379266 Msa0851270:intron 40.0%
TAGGACAAAGAATGTTGTGG+TGG - chr5_4:41379250-41379269 Msa0851270:intron 40.0%
TTATCACCACCTTCACCTTT+AGG + chr5_4:41379284-41379303 None:intergenic 40.0%
AAAGGTGAAGGTGGTGATAA+TGG - chr5_4:41379284-41379303 Msa0851270:intron 40.0%
!! TGATAATGGAAGAGAGGAGA+AGG - chr5_4:41379298-41379317 Msa0851270:intron 40.0%
GAAGGCTGTTATAGAGAATC+GGG - chr5_4:41379346-41379365 Msa0851270:CDS 40.0%
! TATAGAGAATCGGGCTTTTG+TGG - chr5_4:41379355-41379374 Msa0851270:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_4:41379426-41379445 Msa0851270:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_4:41379438-41379457 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_4:41379436-41379455 Msa0851270:CDS 40.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_4:41378632-41378651 Msa0851270:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_4:41378699-41378718 Msa0851270:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_4:41378705-41378724 None:intergenic 45.0%
ATGGCGAATCGTCACAATGA+TGG - chr5_4:41378978-41378997 Msa0851270:CDS 45.0%
TCGTCTGATTCTCCTGGAAA+CGG + chr5_4:41379071-41379090 None:intergenic 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_4:41379139-41379158 Msa0851270:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_4:41379170-41379189 None:intergenic 45.0%
GACAAAGAATGTTGTGGTGG+TGG - chr5_4:41379253-41379272 Msa0851270:intron 45.0%
!! AGGTGGTGATAATGGAAGAG+AGG - chr5_4:41379292-41379311 Msa0851270:intron 45.0%
TAATGGAAGAGAGGAGAAGG+AGG - chr5_4:41379301-41379320 Msa0851270:intron 45.0%
!! AGGAGGTAGAAGCTTTGGAA+AGG - chr5_4:41379318-41379337 Msa0851270:CDS 45.0%
!! GGAGGTAGAAGCTTTGGAAA+GGG - chr5_4:41379319-41379338 Msa0851270:CDS 45.0%
!! GAGGTAGAAGCTTTGGAAAG+GGG - chr5_4:41379320-41379339 Msa0851270:CDS 45.0%
!! AGCTTTGGAAAGGGGTTTGA+AGG - chr5_4:41379328-41379347 Msa0851270:CDS 45.0%
! AATCGGGCTTTTGTGGATTC+TGG - chr5_4:41379362-41379381 Msa0851270:CDS 45.0%
! ATCGGGCTTTTGTGGATTCT+GGG - chr5_4:41379363-41379382 Msa0851270:CDS 45.0%
!!! GCTTTTGTGGATTCTGGGAT+GGG - chr5_4:41379368-41379387 Msa0851270:CDS 45.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_4:41378632-41378651 Msa0851270:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_4:41378699-41378718 Msa0851270:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_4:41378705-41378724 None:intergenic 45.0%
ATGGCGAATCGTCACAATGA+TGG - chr5_4:41378978-41378997 Msa0851270:CDS 45.0%
TCGTCTGATTCTCCTGGAAA+CGG + chr5_4:41379071-41379090 None:intergenic 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_4:41379139-41379158 Msa0851270:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_4:41379170-41379189 None:intergenic 45.0%
GACAAAGAATGTTGTGGTGG+TGG - chr5_4:41379253-41379272 Msa0851270:intron 45.0%
!! AGGTGGTGATAATGGAAGAG+AGG - chr5_4:41379292-41379311 Msa0851270:intron 45.0%
TAATGGAAGAGAGGAGAAGG+AGG - chr5_4:41379301-41379320 Msa0851270:intron 45.0%
!! AGGAGGTAGAAGCTTTGGAA+AGG - chr5_4:41379318-41379337 Msa0851270:CDS 45.0%
!! GGAGGTAGAAGCTTTGGAAA+GGG - chr5_4:41379319-41379338 Msa0851270:CDS 45.0%
!! GAGGTAGAAGCTTTGGAAAG+GGG - chr5_4:41379320-41379339 Msa0851270:CDS 45.0%
!! AGCTTTGGAAAGGGGTTTGA+AGG - chr5_4:41379328-41379347 Msa0851270:CDS 45.0%
! AATCGGGCTTTTGTGGATTC+TGG - chr5_4:41379362-41379381 Msa0851270:CDS 45.0%
! ATCGGGCTTTTGTGGATTCT+GGG - chr5_4:41379363-41379382 Msa0851270:CDS 45.0%
!!! GCTTTTGTGGATTCTGGGAT+GGG - chr5_4:41379368-41379387 Msa0851270:CDS 45.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_4:41378536-41378555 Msa0851270:three_prime_UTR 50.0%
AAGTGGTGCAGCACGTGAAA+CGG - chr5_4:41378934-41378953 Msa0851270:CDS 50.0%
GCACGTGAAACGGTTGAAGA+AGG - chr5_4:41378944-41378963 Msa0851270:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_4:41379002-41379021 Msa0851270:CDS 50.0%
CTATGGAGGTAGCTGAGACA+TGG - chr5_4:41379045-41379064 Msa0851270:CDS 50.0%
GTTTCCAGGAGAATCAGACG+AGG - chr5_4:41379070-41379089 Msa0851270:CDS 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_4:41379089-41379108 Msa0851270:CDS 50.0%
AGTGAGTTATTGCGGCAGAG+AGG - chr5_4:41379097-41379116 Msa0851270:CDS 50.0%
AGTTATTGCGGCAGAGAGGA+AGG - chr5_4:41379101-41379120 Msa0851270:CDS 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_4:41379131-41379150 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_4:41379148-41379167 Msa0851270:intron 50.0%
ACAAGCAATCCGTTCGGTTC+TGG - chr5_4:41379190-41379209 Msa0851270:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_4:41379231-41379250 Msa0851270:intron 50.0%
!! AGAATGTTGTGGTGGTGGAG+TGG - chr5_4:41379258-41379277 Msa0851270:intron 50.0%
GGAGAAGGAGGTAGAAGCTT+TGG - chr5_4:41379313-41379332 Msa0851270:intron 50.0%
!! GGCTTTTGTGGATTCTGGGA+TGG - chr5_4:41379367-41379386 Msa0851270:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_4:41379394-41379413 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_4:41379395-41379414 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_4:41379393-41379412 Msa0851270:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_4:41379394-41379413 Msa0851270:CDS 50.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_4:41378536-41378555 Msa0851270:three_prime_UTR 50.0%
AAGTGGTGCAGCACGTGAAA+CGG - chr5_4:41378934-41378953 Msa0851270:CDS 50.0%
GCACGTGAAACGGTTGAAGA+AGG - chr5_4:41378944-41378963 Msa0851270:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_4:41379002-41379021 Msa0851270:CDS 50.0%
CTATGGAGGTAGCTGAGACA+TGG - chr5_4:41379045-41379064 Msa0851270:CDS 50.0%
GTTTCCAGGAGAATCAGACG+AGG - chr5_4:41379070-41379089 Msa0851270:CDS 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_4:41379089-41379108 Msa0851270:CDS 50.0%
AGTGAGTTATTGCGGCAGAG+AGG - chr5_4:41379097-41379116 Msa0851270:CDS 50.0%
AGTTATTGCGGCAGAGAGGA+AGG - chr5_4:41379101-41379120 Msa0851270:CDS 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_4:41379131-41379150 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_4:41379148-41379167 Msa0851270:intron 50.0%
ACAAGCAATCCGTTCGGTTC+TGG - chr5_4:41379190-41379209 Msa0851270:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_4:41379231-41379250 Msa0851270:intron 50.0%
!! AGAATGTTGTGGTGGTGGAG+TGG - chr5_4:41379258-41379277 Msa0851270:intron 50.0%
GGAGAAGGAGGTAGAAGCTT+TGG - chr5_4:41379313-41379332 Msa0851270:intron 50.0%
!! GGCTTTTGTGGATTCTGGGA+TGG - chr5_4:41379367-41379386 Msa0851270:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_4:41379394-41379413 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_4:41379395-41379414 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_4:41379393-41379412 Msa0851270:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_4:41379394-41379413 Msa0851270:CDS 50.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_4:41378540-41378559 Msa0851270:three_prime_UTR 55.0%
GGCGTCGTTGTTAGCAGAAG+TGG - chr5_4:41378917-41378936 Msa0851270:CDS 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_4:41379012-41379031 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_4:41379011-41379030 Msa0851270:CDS 55.0%
ATGCAGCGGAGAACCTGGTT+CGG - chr5_4:41379016-41379035 Msa0851270:CDS 55.0%
GCAGAGAGGAAGGAGAACCA+CGG - chr5_4:41379111-41379130 Msa0851270:intron 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_4:41379149-41379168 Msa0851270:intron 55.0%
GTCATCATCTCCGCTCGAAC+CGG + chr5_4:41379221-41379240 None:intergenic 55.0%
GGTTCGAGCGGAGATGATGA+CGG - chr5_4:41379220-41379239 Msa0851270:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_4:41379272-41379291 Msa0851270:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_4:41379275-41379294 Msa0851270:intron 55.0%
! TGGGATGGGCCCGATTTTGT+TGG - chr5_4:41379382-41379401 Msa0851270:CDS 55.0%
! GGGATGGGCCCGATTTTGTT+GGG - chr5_4:41379383-41379402 Msa0851270:CDS 55.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_4:41378540-41378559 Msa0851270:three_prime_UTR 55.0%
GGCGTCGTTGTTAGCAGAAG+TGG - chr5_4:41378917-41378936 Msa0851270:CDS 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_4:41379012-41379031 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_4:41379011-41379030 Msa0851270:CDS 55.0%
ATGCAGCGGAGAACCTGGTT+CGG - chr5_4:41379016-41379035 Msa0851270:CDS 55.0%
GCAGAGAGGAAGGAGAACCA+CGG - chr5_4:41379111-41379130 Msa0851270:intron 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_4:41379149-41379168 Msa0851270:intron 55.0%
GTCATCATCTCCGCTCGAAC+CGG + chr5_4:41379221-41379240 None:intergenic 55.0%
GGTTCGAGCGGAGATGATGA+CGG - chr5_4:41379220-41379239 Msa0851270:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_4:41379272-41379291 Msa0851270:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_4:41379275-41379294 Msa0851270:intron 55.0%
! TGGGATGGGCCCGATTTTGT+TGG - chr5_4:41379382-41379401 Msa0851270:CDS 55.0%
! GGGATGGGCCCGATTTTGTT+GGG - chr5_4:41379383-41379402 Msa0851270:CDS 55.0%
GCTGAGACATGGCCGTTTCC+AGG - chr5_4:41379056-41379075 Msa0851270:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+TGG + chr5_4:41379077-41379096 None:intergenic 60.0%
GAGAGGAAGGAGAACCACGG+AGG - chr5_4:41379114-41379133 Msa0851270:intron 60.0%
CCGGTTTCGCCAGAACCGAA+CGG + chr5_4:41379202-41379221 None:intergenic 60.0%
! CCGTTCGGTTCTGGCGAAAC+CGG - chr5_4:41379199-41379218 Msa0851270:intron 60.0%
!! TCTGGCGAAACCGGTTCGAG+CGG - chr5_4:41379208-41379227 Msa0851270:intron 60.0%
!! CGAGCGGAGATGATGACGGT+TGG - chr5_4:41379224-41379243 Msa0851270:intron 60.0%
!! GCGGAGATGATGACGGTTGG+AGG - chr5_4:41379227-41379246 Msa0851270:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_4:41379266-41379285 Msa0851270:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_4:41379387-41379406 Msa0851270:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_4:41379409-41379428 None:intergenic 60.0%
GCTGAGACATGGCCGTTTCC+AGG - chr5_4:41379056-41379075 Msa0851270:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+TGG + chr5_4:41379077-41379096 None:intergenic 60.0%
GAGAGGAAGGAGAACCACGG+AGG - chr5_4:41379114-41379133 Msa0851270:intron 60.0%
CCGGTTTCGCCAGAACCGAA+CGG + chr5_4:41379202-41379221 None:intergenic 60.0%
! CCGTTCGGTTCTGGCGAAAC+CGG - chr5_4:41379199-41379218 Msa0851270:intron 60.0%
!! TCTGGCGAAACCGGTTCGAG+CGG - chr5_4:41379208-41379227 Msa0851270:intron 60.0%
!! CGAGCGGAGATGATGACGGT+TGG - chr5_4:41379224-41379243 Msa0851270:intron 60.0%
!! GCGGAGATGATGACGGTTGG+AGG - chr5_4:41379227-41379246 Msa0851270:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_4:41379266-41379285 Msa0851270:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_4:41379387-41379406 Msa0851270:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_4:41379409-41379428 None:intergenic 60.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_4:41379403-41379422 Msa0851270:CDS 75.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_4:41379403-41379422 Msa0851270:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 41378418 41379617 41378418 ID=Msa0851270;Name=Msa0851270
chr5_4 mRNA 41378418 41379617 41378418 ID=Msa0851270-mRNA-1;Parent=Msa0851270;Name=Msa0851270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=99|1|1|1|1|1|2|148|254
chr5_4 exon 41378418 41379126 41378418 ID=Msa0851270-mRNA-1:exon:13063;Parent=Msa0851270-mRNA-1
chr5_4 exon 41379315 41379617 41379315 ID=Msa0851270-mRNA-1:exon:13062;Parent=Msa0851270-mRNA-1
chr5_4 five_prime_UTR 41379519 41379617 41379519 ID=Msa0851270-mRNA-1:five_prime_utr;Parent=Msa0851270-mRNA-1
chr5_4 CDS 41379315 41379518 41379315 ID=Msa0851270-mRNA-1:cds;Parent=Msa0851270-mRNA-1
chr5_4 CDS 41378566 41379126 41378566 ID=Msa0851270-mRNA-1:cds;Parent=Msa0851270-mRNA-1
chr5_4 three_prime_UTR 41378418 41378565 41378418 ID=Msa0851270-mRNA-1:three_prime_utr;Parent=Msa0851270-mRNA-1
Gene Sequence

>Msa0851270

ATGCTTCCTCCTTTAAGAAGATTCTACGGGTTTGAGAGCTGGATGGATAATAAAGACTATGAGGAAATCAACTGTGACAATTCAAAATCGGAGAGAAAATCAAAAGAAGCATGCAAGAGTCATAGGGAAGCTGAAAGGAGACGCAGACAGAGAATCAACGCTCATCTCAACACTCTTCGCTCTCTCCTTCCTAACACCACAAAGTCAGATAAGGCGTCGTTGTTAGCAGAAGTGGTGCAGCACGTGAAACGGTTGAAGAAGGAAGCTGATGAAATGGCGAATCGTCACAATGATGGAGAGTCTTCCTCTTCATGCAGCGGAGAACCTGGTTCGGTTAATTCTATGGAGGTAGCTGAGACATGGCCGTTTCCAGGAGAATCAGACGAGGCGACAGTGAGTTATTGCGGCAGAGAGGAAGGAGAACCACGGAGGATGAAAGTGACAGTGTGTTGTGAGGATCGACCGGGTCTGAACCATGATCTAACACAAGCAATCCGTTCGGTTCTGGCGAAACCGGTTCGAGCGGAGATGATGACGGTTGGAGGTAGGACAAAGAATGTTGTGGTGGTGGAGTGGCCTAAAGGTGAAGGTGGTGATAATGGAAGAGAGGAGAAGGAGGTAGAAGCTTTGGAAAGGGGTTTGAAGGCTGTTATAGAGAATCGGGCTTTTGTGGATTCTGGGATGGGCCCGATTTTGTTGGGCCGGAAACGGGCTCGTGAGGTTGACTGTTCCTTGTTGTTGAGGAAGAATGAGGACTTTTGCTAA
Protein sequence

>Msa0851270

MLPPLRRFYGFESWMDNKDYEEINCDNSKSERKSKEACKSHREAERRRRQRINAHLNTLRSLLPNTTKSDKASLLAEVVQHVKRLKKEADEMANRHNDGESSSSCSGEPGSVNSMEVAETWPFPGESDEATVSYCGREEGEPRRMKVTVCCEDRPGLNHDLTQAIRSVLAKPVRAEMMTVGGRTKNVVVVEWPKGEGGDNGREEKEVEALERGLKAVIENRAFVDSGMGPILLGRKRAREVDCSLLLRKNEDFC*