AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0777590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777590 MtrunA17_Chr5g0432861 98.100 579 11 0 1 579 1 579 0.0 1173
Msa0777590 MtrunA17_Chr3g0108331 72.241 580 141 7 5 579 4 568 0.0 846
Msa0777590 MtrunA17_Chr5g0401451 39.196 597 295 7 9 551 24 606 7.45e-146 435
Msa0777590 MtrunA17_Chr4g0053841 37.561 615 316 11 13 579 1 595 3.05e-136 409
Msa0777590 MtrunA17_Chr4g0010091 36.348 586 314 8 9 551 16 585 2.97e-126 384
Msa0777590 MtrunA17_Chr1g0195911 34.066 637 320 15 8 579 20 621 1.14e-110 344
Msa0777590 MtrunA17_Chr7g0245201 34.171 597 343 12 20 579 57 640 3.30e-107 335
Msa0777590 MtrunA17_Chr2g0303541 34.753 587 327 10 12 546 12 594 6.01e-107 335
Msa0777590 MtrunA17_Chr7g0268781 33.608 607 306 11 8 549 20 594 7.46e-105 329
Msa0777590 MtrunA17_Chr2g0279481 31.770 661 350 13 13 579 32 685 3.36e-102 323
Msa0777590 MtrunA17_Chr2g0285511 32.833 600 335 11 12 559 12 595 1.15e-100 317
Msa0777590 MtrunA17_Chr4g0050021 34.375 512 280 9 14 471 33 542 1.58e-97 311
Msa0777590 MtrunA17_Chr8g0374011 33.888 661 314 18 17 574 11 651 3.64e-96 307
Msa0777590 MtrunA17_Chr1g0181181 35.084 476 275 7 19 473 20 482 2.51e-94 300
Msa0777590 MtrunA17_Chr2g0320691 34.281 563 314 11 6 516 4 562 3.89e-94 301
Msa0777590 MtrunA17_Chr8g0382231 32.630 521 312 8 25 517 52 561 3.40e-92 296
Msa0777590 MtrunA17_Chr1g0199631 32.545 550 308 14 43 546 8 540 7.04e-83 270
Msa0777590 MtrunA17_Chr4g0076521 31.906 467 288 4 21 470 22 475 3.33e-79 261
Msa0777590 MtrunA17_Chr1g0207461 31.612 484 276 7 22 470 25 488 1.29e-77 257
Msa0777590 MtrunA17_Chr5g0412591 34.362 486 275 9 21 475 22 494 1.11e-74 249
Msa0777590 MtrunA17_Chr1g0191711 28.517 526 329 10 12 517 44 542 3.72e-74 246
Msa0777590 MtrunA17_Chr1g0150191 30.909 495 286 9 12 469 12 487 2.18e-73 246
Msa0777590 MtrunA17_Chr5g0401341 31.667 480 273 9 24 470 26 483 2.38e-72 243
Msa0777590 MtrunA17_Chr8g0367891 33.265 487 260 10 28 475 29 489 7.70e-72 241
Msa0777590 MtrunA17_Chr2g0290741 30.538 465 281 6 24 470 25 465 2.14e-69 234
Msa0777590 MtrunA17_Chr4g0003891 31.250 304 186 4 172 471 30 314 3.41e-44 163
Msa0777590 MtrunA17_Chr2g0332281 31.597 288 180 2 184 471 24 294 6.94e-43 159
Msa0777590 MtrunA17_Chr4g0037661 26.652 469 259 10 69 487 51 484 2.99e-40 154
Msa0777590 MtrunA17_Chr1g0206621 46.622 148 73 3 33 178 76 219 1.94e-38 141
Msa0777590 MtrunA17_Chr1g0163311 24.816 544 301 15 35 516 14 511 2.13e-34 137
Msa0777590 MtrunA17_Chr4g0023801 24.681 470 284 11 35 473 15 445 5.98e-31 127
Msa0777590 MtrunA17_Chr2g0280541 25.564 399 221 12 92 456 2 358 2.93e-24 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777590 AT2G30520.1 67.172 594 173 6 2 579 6 593 0.0 811
Msa0777590 AT2G30520.2 68.058 551 155 5 44 579 1 545 0.0 756
Msa0777590 AT2G30520.3 68.058 551 155 5 44 579 1 545 0.0 756
Msa0777590 AT5G67385.1 38.746 622 304 12 9 579 9 604 2.04e-137 412
Msa0777590 AT5G48800.1 36.273 601 286 11 8 551 22 582 2.66e-120 369
Msa0777590 AT5G67385.4 37.265 585 290 12 46 579 1 559 4.80e-120 366
Msa0777590 AT5G67385.5 37.265 585 290 12 46 579 1 559 4.80e-120 366
Msa0777590 AT5G67385.2 37.265 585 290 12 46 579 1 559 4.80e-120 366
Msa0777590 AT5G67385.3 37.265 585 290 12 46 579 1 559 4.80e-120 366
Msa0777590 AT1G30440.1 33.642 648 338 14 12 575 12 651 3.87e-108 339
Msa0777590 AT3G08570.1 34.423 581 312 14 20 554 30 587 1.15e-105 331
Msa0777590 AT1G03010.1 33.768 613 304 14 8 549 20 601 1.57e-104 328
Msa0777590 AT1G03010.2 34.003 597 293 13 24 549 8 574 4.88e-103 324
Msa0777590 AT2G47860.1 32.886 596 324 9 8 549 21 594 1.04e-102 324
Msa0777590 AT1G03010.3 34.003 597 293 13 24 549 34 600 1.08e-102 324
Msa0777590 AT3G49970.1 34.813 563 302 14 33 579 2 515 2.79e-98 308
Msa0777590 AT3G49970.2 34.813 563 302 14 33 579 2 515 2.79e-98 308
Msa0777590 AT2G47860.3 31.664 619 324 10 8 549 21 617 1.87e-97 311
Msa0777590 AT5G64330.2 32.099 567 273 7 15 471 42 606 2.67e-94 303
Msa0777590 AT3G08660.1 33.274 565 307 16 20 551 30 557 1.22e-93 298
Msa0777590 AT5G64330.1 32.099 567 273 7 15 471 42 606 1.44e-93 303
Msa0777590 AT5G13600.1 32.394 568 350 8 19 557 20 582 2.76e-93 298
Msa0777590 AT5G03250.1 32.681 511 288 8 10 476 10 508 5.70e-90 289
Msa0777590 AT3G08660.3 32.645 533 290 15 52 551 1 497 2.75e-85 275
Msa0777590 AT5G64330.3 31.667 540 257 7 42 471 10 547 3.51e-85 277
Msa0777590 AT3G44820.1 31.059 557 324 10 19 516 17 572 2.20e-84 276
Msa0777590 AT5G10250.2 32.418 546 321 11 6 517 27 558 1.43e-82 271
Msa0777590 AT5G66560.1 32.528 538 266 10 17 472 10 532 2.32e-81 269
Msa0777590 AT3G50840.1 33.708 534 302 11 11 516 1 510 3.06e-81 266
Msa0777590 AT5G10250.1 33.086 538 313 12 6 517 27 543 3.26e-81 267
Msa0777590 AT2G47860.4 34.305 446 238 8 8 420 21 444 5.33e-80 259
Msa0777590 AT5G66560.3 32.689 517 252 9 37 472 2 503 3.68e-77 257
Msa0777590 AT5G66560.4 34.989 443 236 7 67 472 66 493 8.15e-77 256
Msa0777590 AT2G47860.6 32.623 469 238 9 8 420 21 467 7.23e-75 246
Msa0777590 AT3G50840.2 33.735 498 279 10 46 516 1 474 2.07e-72 241
Msa0777590 AT3G50840.3 33.735 498 279 10 46 516 1 474 2.07e-72 241
Msa0777590 AT5G47800.3 29.814 483 306 5 20 483 21 489 8.21e-72 241
Msa0777590 AT5G47800.1 29.814 483 306 5 20 483 21 489 8.21e-72 241
Msa0777590 AT5G47800.4 29.814 483 306 5 20 483 21 489 8.21e-72 241
Msa0777590 AT1G67900.5 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT1G67900.1 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT1G67900.3 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT1G67900.6 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT1G67900.2 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT1G67900.4 33.269 517 287 7 22 491 23 528 1.18e-71 242
Msa0777590 AT4G31820.1 30.189 477 269 7 24 470 26 468 6.01e-71 238
Msa0777590 AT5G47800.2 29.814 483 306 5 20 483 134 602 1.37e-70 240
Msa0777590 AT2G47860.5 30.162 494 273 7 110 549 1 476 1.12e-68 231
Msa0777590 AT2G47860.2 30.162 494 273 7 110 549 1 476 1.12e-68 231
Msa0777590 AT3G08660.2 32.143 420 239 11 20 423 30 419 2.92e-68 228
Msa0777590 AT2G14820.2 30.103 485 279 9 24 470 26 488 2.66e-67 230
Msa0777590 AT2G14820.1 30.103 485 279 9 24 470 26 488 2.66e-67 230
Msa0777590 AT5G67440.3 30.498 482 287 9 22 477 24 483 2.43e-66 226
Msa0777590 AT5G67440.1 30.498 482 287 9 22 477 24 483 2.43e-66 226
Msa0777590 AT2G23050.1 29.741 464 254 9 38 476 40 456 8.84e-63 214
Msa0777590 AT5G66560.2 31.070 486 238 10 17 420 10 480 1.22e-62 214
Msa0777590 AT5G03250.2 30.392 408 231 6 110 476 1 396 3.93e-62 213
Msa0777590 AT4G31820.2 29.176 449 254 7 52 470 1 415 2.42e-61 211
Msa0777590 AT5G67440.2 30.388 464 275 9 40 477 5 446 3.38e-61 212
Msa0777590 AT4G37590.1 29.135 532 294 11 22 509 24 516 1.16e-60 211
Msa0777590 AT3G26490.1 27.955 533 293 10 22 494 23 524 2.74e-59 207
Msa0777590 AT3G26490.3 27.955 533 293 10 22 494 23 524 2.74e-59 207
Msa0777590 AT3G26490.4 27.955 533 293 10 22 494 23 524 2.74e-59 207
Msa0777590 AT3G26490.2 27.706 462 250 9 22 423 23 460 6.64e-46 169
Msa0777590 AT5G48130.1 27.423 485 269 14 19 469 33 468 1.34e-45 171
Msa0777590 AT3G19850.1 25.100 502 293 8 35 488 14 480 5.78e-43 162
Msa0777590 AT1G52770.1 31.597 288 186 3 184 471 28 304 4.59e-42 157
Msa0777590 AT3G15570.1 33.000 300 170 6 184 471 29 309 2.65e-41 155
Msa0777590 AT2G23050.2 27.649 387 213 8 110 476 1 340 1.64e-40 151
Msa0777590 AT1G50280.1 25.887 479 274 10 35 464 14 460 1.21e-37 146
Msa0777590 AT1G50280.2 26.471 442 249 9 67 464 18 427 3.30e-37 144
Msa0777590 AT3G03510.1 25.316 474 298 11 79 546 8 431 1.13e-34 137
Msa0777590 AT5G17580.2 24.792 480 297 9 27 478 22 465 5.59e-33 133
Msa0777590 AT5G17580.1 24.895 474 292 9 27 472 7 444 7.25e-33 133
Msa0777590 AT3G22104.1 26.237 465 257 13 27 455 6 420 2.20e-31 128
Msa0777590 AT3G22104.4 24.936 393 212 10 96 455 3 345 3.48e-22 99.8
Msa0777590 AT3G22104.2 24.936 393 212 10 96 455 3 345 3.48e-22 99.8
Msa0777590 AT3G22104.3 24.936 393 212 10 96 455 3 345 3.48e-22 99.8
Msa0777590 AT3G49900.2 29.464 224 130 9 10 222 49 255 5.15e-13 72.0
Msa0777590 AT3G49900.1 28.632 234 139 9 10 232 49 265 5.21e-13 72.0

Find 165 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTCCGTGTCACGTTCC+AGG 0.240654 5_2:+56672593 None:intergenic
ATGGAGAACCTTCTCCTTCT+AGG 0.245811 5_2:+56672310 None:intergenic
CTGCTTTCTCAAAAGCTTCT+TGG 0.250098 5_2:+56674007 None:intergenic
CTATAAAATGCAATTTGTTT+GGG 0.268314 5_2:-56672171 Msa0777590:three_prime_UTR
TCGTCCGTGTCAAAGAAATT+AGG 0.274325 5_2:-56672406 Msa0777590:CDS
ACTATAAAATGCAATTTGTT+TGG 0.275295 5_2:-56672172 Msa0777590:three_prime_UTR
AACGGTAGTACTAGTTTATA+TGG 0.278745 5_2:+56674723 None:intergenic
CTACGACGGTGAGAGGCTTC+TGG 0.311434 5_2:-56673260 Msa0777590:CDS
AGTTGAGAACGGAGTTGATT+AGG 0.340136 5_2:-56672525 Msa0777590:CDS
TGCTGTCGATATTTATCTTA+AGG 0.341842 5_2:-56672996 Msa0777590:intron
AGTACTAGTTTATATGGTTA+TGG 0.352191 5_2:+56674729 None:intergenic
TTTCGCGCCACGTTGTTTCA+TGG 0.353851 5_2:+56673627 None:intergenic
CTACATCAGAAAGCTGATAA+TGG 0.355121 5_2:-56674082 Msa0777590:CDS
TCAACGCTCCGGCGAGAGTT+AGG 0.356655 5_2:+56673602 None:intergenic
TTATGGTGGATTGTTGTAAT+AGG 0.365816 5_2:+56674746 None:intergenic
AAGAAAACCTTCAGTTCTTC+CGG 0.369455 5_2:+56673873 None:intergenic
GTTTAGTATGGCGGGTTTGT+TGG 0.376307 5_2:-56672131 Msa0777590:three_prime_UTR
TTCTGGATCTTGACAGCATT+CGG 0.378888 5_2:-56673243 Msa0777590:CDS
GTATGGCGGGTTTGTTGGTT+AGG 0.379064 5_2:-56672126 Msa0777590:three_prime_UTR
GTCGCCTTAACAACTCTTTC+CGG 0.380845 5_2:-56673844 Msa0777590:CDS
TAGGTAAGCGTCCACAGTTT+TGG 0.383838 5_2:+56673120 None:intergenic
TTGTAGAATCTCACGTTGTT+CGG 0.393991 5_2:+56673462 None:intergenic
CTCAAAAGCTTCTTGGCCGC+CGG 0.395497 5_2:+56674014 None:intergenic
AAACAAAATAATCTATGAAA+TGG 0.401564 5_2:+56672291 None:intergenic
ACGGGTGGCGGAAGTGGCGG+TGG 0.402393 5_2:-56673535 Msa0777590:CDS
AAAACAATAACGTAAATTCA+TGG 0.405207 5_2:-56674665 Msa0777590:exon
CTCTTCCGTGTCACGTTCCA+GGG 0.406974 5_2:+56672594 None:intergenic
GTCTCAGAACAAACGATTAC+CGG 0.410250 5_2:-56672674 Msa0777590:CDS
TGCACTTAAACTTTCATATG+AGG 0.412052 5_2:-56672710 Msa0777590:CDS
AACATCATCATAGTTTAGTA+TGG 0.413333 5_2:-56672143 Msa0777590:three_prime_UTR
TCTCTTGCCATGGAGAGAAC+AGG 0.413490 5_2:-56674619 Msa0777590:CDS
AGAGAGAGTCTGCTAGGATT+AGG 0.417337 5_2:+56674637 None:intergenic
AACATATCAACTTTGTGTCT+TGG 0.420123 5_2:-56674852 Msa0777590:five_prime_UTR
TGGATTTGACAAAGCCTAGA+AGG 0.433974 5_2:-56672324 Msa0777590:CDS
GGTGGCGGAAGTGGCGGTGG+AGG 0.435544 5_2:-56673532 Msa0777590:CDS
CCATTGCCCTGTTCTCTCCA+TGG 0.437876 5_2:+56674612 None:intergenic
CTAGCCGATCACCGCCGAAT+TGG 0.439522 5_2:-56673705 Msa0777590:CDS
GTGGCGGTGGAGGAAAATTT+CGG 0.443249 5_2:-56673522 Msa0777590:CDS
GATCAAACGGGTGGCGGAAG+TGG 0.444216 5_2:-56673541 Msa0777590:CDS
AGCTCCTCCGTCCACCAATT+CGG 0.447590 5_2:+56673691 None:intergenic
TTCAACGCCGGTAGCACGTT+CGG 0.451318 5_2:-56673175 Msa0777590:CDS
TCAAAAGCTTCTTGGCCGCC+GGG 0.451799 5_2:+56674015 None:intergenic
TTCTTGGCCGCCGGGGATGT+CGG 0.452645 5_2:+56674023 None:intergenic
GTTCTTGACGTTGAATCCTT+CGG 0.454031 5_2:-56673664 Msa0777590:CDS
CTTCTCTAACTCATCAAGAT+TGG 0.455111 5_2:+56672756 None:intergenic
ATGTTTAGCTCGTCGGCGAC+GGG 0.456225 5_2:+56673784 None:intergenic
GCCGATCACCGCCGAATTGG+TGG 0.462783 5_2:-56673702 Msa0777590:CDS
GATAAGAAGCGGGGCAACCC+TGG 0.463981 5_2:-56672611 Msa0777590:CDS
ATCAATTAAAGATAAGAAGC+GGG 0.464447 5_2:-56672621 Msa0777590:CDS
ACGGCAATTATTAAGTGAAA+AGG 0.465717 5_2:-56674876 Msa0777590:five_prime_UTR
TCCACCAATTCGGCGGTGAT+CGG 0.469096 5_2:+56673701 None:intergenic
GAGGTCTAATCTGGTGAGTT+CGG 0.469709 5_2:+56674047 None:intergenic
TCATTCTTATTTCCATGACT+TGG 0.472818 5_2:+56672009 None:intergenic
GGCGGAACAACTTCATCGTC+TGG 0.474475 5_2:-56672463 Msa0777590:CDS
CATTTCTGCATTCGTCGAAA+AGG 0.476321 5_2:-56673215 Msa0777590:CDS
TACCTTGTGGAGAGAATAAG+TGG 0.481583 5_2:+56674428 None:intergenic
ACAATGCATGCAGTACAATC+TGG 0.482856 5_2:+56672650 None:intergenic
GATGTCGGAGAGGTCTAATC+TGG 0.483299 5_2:+56674038 None:intergenic
GTTACAAACAGACGTAACTT+TGG 0.492774 5_2:-56672563 Msa0777590:CDS
TTTGGTCTGAAATTGTTATG+TGG 0.497692 5_2:+56674691 None:intergenic
TAACAATGAAGTTAATCCAC+TGG 0.499013 5_2:-56674781 Msa0777590:five_prime_UTR
TCATCATAGTTTAGTATGGC+GGG 0.499235 5_2:-56672139 Msa0777590:three_prime_UTR
ACTAGTTTATATGGTTATGG+TGG 0.500487 5_2:+56674732 None:intergenic
GGAGAAAGTGTGCAGTGTGA+TGG 0.507183 5_2:-56672734 Msa0777590:CDS
GTCTTTGTATCAAAGAAGAA+AGG 0.507825 5_2:-56672055 Msa0777590:three_prime_UTR
ACAACTTCATCGTCTGGAGT+TGG 0.509583 5_2:-56672457 Msa0777590:CDS
AGCGCCGGAAAGAGTTGTTA+AGG 0.510204 5_2:+56673840 None:intergenic
TAAAGATACGTCTCATATTG+AGG 0.512243 5_2:-56672356 Msa0777590:CDS
GGAGCTTGTACGTGATCAAA+CGG 0.517849 5_2:-56673554 Msa0777590:CDS
CAAACGGGTGGCGGAAGTGG+CGG 0.520456 5_2:-56673538 Msa0777590:CDS
CAACGACGCCACGTTATTGA+CGG 0.520841 5_2:+56673951 None:intergenic
GATGTTTAGCTCGTCGGCGA+CGG 0.522709 5_2:+56673783 None:intergenic
AAATGCAATTTGTTTGGGGT+AGG 0.531408 5_2:-56672166 Msa0777590:three_prime_UTR
AAAGCAGCTAAATTCTGTTA+CGG 0.532489 5_2:-56673991 Msa0777590:CDS
TATATACGTTTGCCAAGTCA+TGG 0.534360 5_2:-56672021 Msa0777590:three_prime_UTR
CTTTACTACAAGCAAGATAA+CGG 0.536104 5_2:-56674895 Msa0777590:five_prime_UTR
ATTGCCATTCTCATTCCAAA+AGG 0.536892 5_2:-56673049 Msa0777590:CDS
AATTCGGCGGTGATCGGCTA+GGG 0.539024 5_2:+56673707 None:intergenic
AATACTCGTCGGTCATCTGA+AGG 0.539863 5_2:+56673911 None:intergenic
CTCTGCCGCCATGCAACGCG+TGG 0.540203 5_2:-56673143 Msa0777590:CDS
AACAATGAAGTTAATCCACT+GGG 0.542253 5_2:-56674780 Msa0777590:five_prime_UTR
GTTTCAGATTTGCAAAAGAA+CGG 0.543872 5_2:-56672490 Msa0777590:CDS
AATACCGTTGAACTTCGAAA+TGG 0.548610 5_2:+56673069 None:intergenic
CAATTCGGCGGTGATCGGCT+AGG 0.550046 5_2:+56673706 None:intergenic
AAGCTGAATCCGTTCAAGAA+TGG 0.550799 5_2:-56672382 Msa0777590:CDS
TGTTGACGCCGTCAGCTCAA+AGG 0.551051 5_2:-56673749 Msa0777590:CDS
GAAGGATTCAACGTCAAGAA+CGG 0.559469 5_2:+56673666 None:intergenic
ATTGCAGACCTTTGAGCTGA+CGG 0.559999 5_2:+56673741 None:intergenic
TTTGATCACGTACAAGCTCC+CGG 0.560599 5_2:+56673557 None:intergenic
CGTAGGAAGCAATCTCAGCT+AGG 0.562734 5_2:+56673101 None:intergenic
TCTTTAGAACCATTCTTGAA+CGG 0.565852 5_2:+56672373 None:intergenic
GGCAACCCTGGAACGTGACA+CGG 0.566473 5_2:-56672599 Msa0777590:CDS
ATTGTAACTATAACATCACT+TGG 0.566826 5_2:+56674459 None:intergenic
GAGCTTGTACGTGATCAAAC+GGG 0.569814 5_2:-56673553 Msa0777590:CDS
AGACAGCAGAGGAAGTTGAT+CGG 0.571060 5_2:+56673400 None:intergenic
TTTGACGATGTTTAGCTCGT+CGG 0.573492 5_2:+56673777 None:intergenic
CTCTTGCCATGGAGAGAACA+GGG 0.575153 5_2:-56674618 Msa0777590:CDS
ATACACAGAACGCACACTCC+GGG 0.575932 5_2:-56673575 Msa0777590:CDS
TGTGTATGCAATCAACGCTC+CGG 0.576129 5_2:+56673591 None:intergenic
AACCTCGCCGGAAGAACTGA+AGG 0.576780 5_2:-56673880 Msa0777590:CDS
TAGCAGACTCTCTCTTGCCA+TGG 0.578808 5_2:-56674629 Msa0777590:CDS
TGACAAAGCCTAGAAGGAGA+AGG 0.583766 5_2:-56672318 Msa0777590:CDS
CTTACCTAATTTCTTTGACA+CGG 0.586200 5_2:+56672402 None:intergenic
GCATGCAGTACAATCTGGAC+CGG 0.587503 5_2:+56672655 None:intergenic
ATGGCAAGAGAGAGTCTGCT+AGG 0.588044 5_2:+56674631 None:intergenic
TATAAAATGCAATTTGTTTG+GGG 0.589000 5_2:-56672170 Msa0777590:three_prime_UTR
CTAGCTGAGATTGCTTCCTA+CGG 0.589130 5_2:-56673100 Msa0777590:CDS
ACGTCTCATATTGAGGATGT+TGG 0.590809 5_2:-56672349 Msa0777590:CDS
GCGAAATACCTAACTCTCGC+CGG 0.591461 5_2:-56673610 Msa0777590:CDS
TTAGACCTCTCCGACATCCC+CGG 0.592064 5_2:-56674033 Msa0777590:CDS
CGAAATGGTGAGTTCTCCGT+AGG 0.593883 5_2:+56673084 None:intergenic
GATCAATTAAAGATAAGAAG+CGG 0.594149 5_2:-56672622 Msa0777590:CDS
TTCACGCTTGCACGAGCTAG+AGG 0.595491 5_2:+56673351 None:intergenic
CATACACAGAACGCACACTC+CGG 0.595804 5_2:-56673576 Msa0777590:CDS
GTACGTGATCAAACGGGTGG+CGG 0.599151 5_2:-56673547 Msa0777590:CDS
CATCGTCTCTCTCTTTCCGT+CGG 0.601369 5_2:-56673437 Msa0777590:CDS
GATTTGCAAAAGAACGGTCA+CGG 0.603295 5_2:-56672484 Msa0777590:CDS
CTCACCATTTCGAAGTTCAA+CGG 0.607753 5_2:-56673073 Msa0777590:CDS
TTGTAACTATAACATCACTT+GGG 0.608092 5_2:+56674460 None:intergenic
ATCATCATAGTTTAGTATGG+CGG 0.608805 5_2:-56672140 Msa0777590:three_prime_UTR
CGGCGACGGGGAGAAGCTGA+CGG 0.610463 5_2:+56673797 None:intergenic
AGAGAACGAGGAGTTGAGAA+CGG 0.616083 5_2:-56672536 Msa0777590:CDS
TCCTCCGTCCACCAATTCGG+CGG 0.617815 5_2:+56673694 None:intergenic
CGAGATAACCGTCAATAACG+TGG 0.619386 5_2:-56673959 Msa0777590:CDS
AACCTTCAGTTCTTCCGGCG+AGG 0.620572 5_2:+56673878 None:intergenic
CAACATGTAACAGTTCATGC+TGG 0.620723 5_2:-56674118 Msa0777590:intron
TATTGCGACGGAAACCTCGC+CGG 0.621897 5_2:-56673892 Msa0777590:CDS
GCAACGCGTGGCCAAAACTG+TGG 0.622071 5_2:-56673131 Msa0777590:CDS
CAATCTTGATGAGTTAGAGA+AGG 0.623085 5_2:-56672755 Msa0777590:CDS
TGGAACGTGACACGGAAGAG+AGG 0.623494 5_2:-56672591 Msa0777590:CDS
GGCCGCCGGGGATGTCGGAG+AGG 0.623886 5_2:+56674028 None:intergenic
CATTCACCTACGACGGTGAG+AGG 0.625301 5_2:-56673267 Msa0777590:CDS
CCATGGAGAGAACAGGGCAA+TGG 0.627320 5_2:-56674612 Msa0777590:intron
GGAAAAGCAGCTTTCTCCGA+CGG 0.630312 5_2:+56673421 None:intergenic
CGCGCCACGTTGTTTCATGG+CGG 0.633037 5_2:+56673630 None:intergenic
CGACGGAAAGAGAGAGACGA+TGG 0.633783 5_2:+56673438 None:intergenic
ATCACCGCCGAATTGGTGGA+CGG 0.634104 5_2:-56673698 Msa0777590:CDS
CAGAAGCCTCTCACCGTCGT+AGG 0.635598 5_2:+56673261 None:intergenic
CAAAAGCTTCTTGGCCGCCG+GGG 0.637469 5_2:+56674016 None:intergenic
TTTGGCCACGCGTTGCATGG+CGG 0.640820 5_2:+56673138 None:intergenic
TCATATTGAGGATGTTGGTG+TGG 0.644673 5_2:-56672344 Msa0777590:CDS
CATGTAACAGTTCATGCTGG+TGG 0.652427 5_2:-56674115 Msa0777590:intron
TAAGATAAATATCGACAGCA+CGG 0.663459 5_2:+56672999 None:intergenic
GTTTCCGTCGCAATACTCGT+CGG 0.664196 5_2:+56673900 None:intergenic
GTCGGTCATCTGAAGGAACA+CGG 0.668856 5_2:+56673918 None:intergenic
AACTTTGGTGAGAGAGAACG+AGG 0.672959 5_2:-56672548 Msa0777590:CDS
AGCCACTTATTCTCTCCACA+AGG 0.673761 5_2:-56674430 Msa0777590:intron
GTTCTGTCATTCACCTACGA+CGG 0.675092 5_2:-56673274 Msa0777590:CDS
CACGTAGGTAAATAGCGCAA+CGG 0.677467 5_2:+56673374 None:intergenic
CGCAACGGAGAAGACAGCAG+AGG 0.680863 5_2:+56673389 None:intergenic
TGACACGGACGAAAAGAATG+TGG 0.683709 5_2:+56672417 None:intergenic
AGCACCACAGTGTTCAACGC+CGG 0.685789 5_2:-56673187 Msa0777590:CDS
TCAATTAAAGATAAGAAGCG+GGG 0.686432 5_2:-56672620 Msa0777590:CDS
ACCGCCGAATTGGTGGACGG+AGG 0.689811 5_2:-56673695 Msa0777590:CDS
CTTGTACGTGATCAAACGGG+TGG 0.696840 5_2:-56673550 Msa0777590:CDS
ATGACCGACGAGTATTGCGA+CGG 0.697651 5_2:-56673904 Msa0777590:CDS
TTGCAAAAGAACGGTCACGG+CGG 0.698644 5_2:-56672481 Msa0777590:CDS
TAGGTAATGTAGAACCCCAG+TGG 0.711562 5_2:+56674765 None:intergenic
GACCTCTCCGACATCCCCGG+CGG 0.736635 5_2:-56674030 Msa0777590:CDS
GACATGTAAGTTTGTCACTG+TGG 0.753540 5_2:+56674828 None:intergenic
TGCACGAGCTAGAGGCACGT+AGG 0.757887 5_2:+56673359 None:intergenic
GCTACCGGCGTTGAACACTG+TGG 0.775866 5_2:+56673183 None:intergenic
ACAATGAAGTTAATCCACTG+GGG 0.781409 5_2:-56674779 Msa0777590:five_prime_UTR
ATCACCGCCATGAAACAACG+TGG 0.794760 5_2:-56673634 Msa0777590:CDS
GGTCATCTGAAGGAACACGG+CGG 0.837339 5_2:+56673921 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATATAAGATAAAGT+TGG + chr5_2:56673994-56674013 None:intergenic 10.0%
!!! ATAAATTTTAACATAACATG+TGG - chr5_2:56674040-56674059 Msa0777590:CDS 15.0%
!! AAACAAAATAATCTATGAAA+TGG + chr5_2:56674555-56674574 None:intergenic 15.0%
!!! TTACGTTATTGTTTTTCTTT+TGG + chr5_2:56672173-56672192 None:intergenic 20.0%
!! AAAACAATAACGTAAATTCA+TGG - chr5_2:56672178-56672197 Msa0777590:three_prime_UTR 20.0%
!! AAATGATTAATCATATTACG+AGG - chr5_2:56674017-56674036 Msa0777590:CDS 20.0%
!!! ACTATAAAATGCAATTTGTT+TGG - chr5_2:56674671-56674690 Msa0777590:five_prime_UTR 20.0%
!!! CTATAAAATGCAATTTGTTT+GGG - chr5_2:56674672-56674691 Msa0777590:five_prime_UTR 20.0%
!!! TATAAAATGCAATTTGTTTG+GGG - chr5_2:56674673-56674692 Msa0777590:five_prime_UTR 20.0%
!! AGTACTAGTTTATATGGTTA+TGG + chr5_2:56672117-56672136 None:intergenic 25.0%
! CTTGAAACTAAAACAACTAT+GGG + chr5_2:56672302-56672321 None:intergenic 25.0%
! ACTTGAAACTAAAACAACTA+TGG + chr5_2:56672303-56672322 None:intergenic 25.0%
! TTGTAACTATAACATCACTT+GGG + chr5_2:56672386-56672405 None:intergenic 25.0%
! ATTGTAACTATAACATCACT+TGG + chr5_2:56672387-56672406 None:intergenic 25.0%
!! TAGTAGTTTTTAATACCTTG+TGG + chr5_2:56672431-56672450 None:intergenic 25.0%
! GATCAATTAAAGATAAGAAG+CGG - chr5_2:56674221-56674240 Msa0777590:intron 25.0%
! ATCAATTAAAGATAAGAAGC+GGG - chr5_2:56674222-56674241 Msa0777590:intron 25.0%
! AACATCATCATAGTTTAGTA+TGG - chr5_2:56674700-56674719 Msa0777590:five_prime_UTR 25.0%
CTTTACTACAAGCAAGATAA+CGG - chr5_2:56671948-56671967 Msa0777590:three_prime_UTR 30.0%
ACGGCAATTATTAAGTGAAA+AGG - chr5_2:56671967-56671986 Msa0777590:three_prime_UTR 30.0%
AACATATCAACTTTGTGTCT+TGG - chr5_2:56671991-56672010 Msa0777590:three_prime_UTR 30.0%
TAACAATGAAGTTAATCCAC+TGG - chr5_2:56672062-56672081 Msa0777590:three_prime_UTR 30.0%
AACAATGAAGTTAATCCACT+GGG - chr5_2:56672063-56672082 Msa0777590:three_prime_UTR 30.0%
!! TTATGGTGGATTGTTGTAAT+AGG + chr5_2:56672100-56672119 None:intergenic 30.0%
! ACTAGTTTATATGGTTATGG+TGG + chr5_2:56672114-56672133 None:intergenic 30.0%
! AACGGTAGTACTAGTTTATA+TGG + chr5_2:56672123-56672142 None:intergenic 30.0%
GTTATGTGGTTTGAGAAAAA+CGG + chr5_2:56672141-56672160 None:intergenic 30.0%
! TTTGGTCTGAAATTGTTATG+TGG + chr5_2:56672155-56672174 None:intergenic 30.0%
CATCTGATTAAAGATGTGTT+CGG + chr5_2:56672623-56672642 None:intergenic 30.0%
AAAGCAGCTAAATTCTGTTA+CGG - chr5_2:56672852-56672871 Msa0777590:intron 30.0%
!!! TTCGGATTTTGTTTCTGAAT+CGG + chr5_2:56673366-56673385 None:intergenic 30.0%
TAAGATAAATATCGACAGCA+CGG + chr5_2:56673847-56673866 None:intergenic 30.0%
TGCTGTCGATATTTATCTTA+AGG - chr5_2:56673847-56673866 Msa0777590:CDS 30.0%
GAGTTGAAGGAAACATTTAT+TGG + chr5_2:56673875-56673894 None:intergenic 30.0%
TGCACTTAAACTTTCATATG+AGG - chr5_2:56674133-56674152 Msa0777590:intron 30.0%
TCAATTAAAGATAAGAAGCG+GGG - chr5_2:56674223-56674242 Msa0777590:intron 30.0%
GTTTCAGATTTGCAAAAGAA+CGG - chr5_2:56674353-56674372 Msa0777590:intron 30.0%
CTTACCTAATTTCTTTGACA+CGG + chr5_2:56674444-56674463 None:intergenic 30.0%
!! TCTTTAGAACCATTCTTGAA+CGG + chr5_2:56674473-56674492 None:intergenic 30.0%
TAAAGATACGTCTCATATTG+AGG - chr5_2:56674487-56674506 Msa0777590:intron 30.0%
ATCATCATAGTTTAGTATGG+CGG - chr5_2:56674703-56674722 Msa0777590:five_prime_UTR 30.0%
GTCTTTGTATCAAAGAAGAA+AGG - chr5_2:56674788-56674807 Msa0777590:five_prime_UTR 30.0%
! TCATTCTTATTTCCATGACT+TGG + chr5_2:56674837-56674856 None:intergenic 30.0%
ACAATGAAGTTAATCCACTG+GGG - chr5_2:56672064-56672083 Msa0777590:three_prime_UTR 35.0%
TGAAAGTGAAAGAGAAGAAG+AGG + chr5_2:56672277-56672296 None:intergenic 35.0%
! TTTGTTGCTTGCGTTATTTC+AGG - chr5_2:56672343-56672362 Msa0777590:CDS 35.0%
ATGATTAGTGTTAGAGACAC+CGG - chr5_2:56672590-56672609 Msa0777590:CDS 35.0%
AAGCAAGCACAAAACTATGA+AGG + chr5_2:56672664-56672683 None:intergenic 35.0%
CTACATCAGAAAGCTGATAA+TGG - chr5_2:56672761-56672780 Msa0777590:CDS 35.0%
AAGAAAACCTTCAGTTCTTC+CGG + chr5_2:56672973-56672992 None:intergenic 35.0%
! AACTGAAGGTTTTCTTTCTC+AGG - chr5_2:56672977-56672996 Msa0777590:intron 35.0%
TTGTAGAATCTCACGTTGTT+CGG + chr5_2:56673384-56673403 None:intergenic 35.0%
AATACCGTTGAACTTCGAAA+TGG + chr5_2:56673777-56673796 None:intergenic 35.0%
ATTGCCATTCTCATTCCAAA+AGG - chr5_2:56673794-56673813 Msa0777590:CDS 35.0%
ATGTTTCCTTCAACTCACTT+CGG - chr5_2:56673879-56673898 Msa0777590:CDS 35.0%
CTCACTTCGGTCTTAAATAT+AGG - chr5_2:56673892-56673911 Msa0777590:CDS 35.0%
CTTCTCTAACTCATCAAGAT+TGG + chr5_2:56674090-56674109 None:intergenic 35.0%
CAATCTTGATGAGTTAGAGA+AGG - chr5_2:56674088-56674107 Msa0777590:CDS 35.0%
GTTACAAACAGACGTAACTT+TGG - chr5_2:56674280-56674299 Msa0777590:intron 35.0%
! AAATGCAATTTGTTTGGGGT+AGG - chr5_2:56674677-56674696 Msa0777590:five_prime_UTR 35.0%
TCATCATAGTTTAGTATGGC+GGG - chr5_2:56674704-56674723 Msa0777590:five_prime_UTR 35.0%
TATATACGTTTGCCAAGTCA+TGG - chr5_2:56674822-56674841 Msa0777590:five_prime_UTR 35.0%
GACATGTAAGTTTGTCACTG+TGG + chr5_2:56672018-56672037 None:intergenic 40.0%
TACCTTGTGGAGAGAATAAG+TGG + chr5_2:56672418-56672437 None:intergenic 40.0%
CAACATGTAACAGTTCATGC+TGG - chr5_2:56672725-56672744 Msa0777590:CDS 40.0%
CTGCTTTCTCAAAAGCTTCT+TGG + chr5_2:56672839-56672858 None:intergenic 40.0%
!!! ACGGCATGATTTTAGAACGA+CGG + chr5_2:56673030-56673049 None:intergenic 40.0%
! TTTGACGATGTTTAGCTCGT+CGG + chr5_2:56673069-56673088 None:intergenic 40.0%
GAAGGATTCAACGTCAAGAA+CGG + chr5_2:56673180-56673199 None:intergenic 40.0%
GTTCTTGACGTTGAATCCTT+CGG - chr5_2:56673179-56673198 Msa0777590:CDS 40.0%
TTCTGGATCTTGACAGCATT+CGG - chr5_2:56673600-56673619 Msa0777590:CDS 40.0%
CATTTCTGCATTCGTCGAAA+AGG - chr5_2:56673628-56673647 Msa0777590:CDS 40.0%
CTCACCATTTCGAAGTTCAA+CGG - chr5_2:56673770-56673789 Msa0777590:CDS 40.0%
! TTAAGACCGAAGTGAGTTGA+AGG + chr5_2:56673888-56673907 None:intergenic 40.0%
GTCTCAGAACAAACGATTAC+CGG - chr5_2:56674169-56674188 Msa0777590:intron 40.0%
ACAATGCATGCAGTACAATC+TGG + chr5_2:56674196-56674215 None:intergenic 40.0%
! AGTTGAGAACGGAGTTGATT+AGG - chr5_2:56674318-56674337 Msa0777590:intron 40.0%
GATTTGCAAAAGAACGGTCA+CGG - chr5_2:56674359-56674378 Msa0777590:intron 40.0%
TCGTCCGTGTCAAAGAAATT+AGG - chr5_2:56674437-56674456 Msa0777590:CDS 40.0%
AAGCTGAATCCGTTCAAGAA+TGG - chr5_2:56674461-56674480 Msa0777590:CDS 40.0%
ACGTCTCATATTGAGGATGT+TGG - chr5_2:56674494-56674513 Msa0777590:intron 40.0%
TCATATTGAGGATGTTGGTG+TGG - chr5_2:56674499-56674518 Msa0777590:intron 40.0%
TGGATTTGACAAAGCCTAGA+AGG - chr5_2:56674519-56674538 Msa0777590:intron 40.0%
TAGGTAATGTAGAACCCCAG+TGG + chr5_2:56672081-56672100 None:intergenic 45.0%
AGAGAGAGTCTGCTAGGATT+AGG + chr5_2:56672209-56672228 None:intergenic 45.0%
AGCCACTTATTCTCTCCACA+AGG - chr5_2:56672413-56672432 Msa0777590:CDS 45.0%
CATGTAACAGTTCATGCTGG+TGG - chr5_2:56672728-56672747 Msa0777590:CDS 45.0%
GAGGTCTAATCTGGTGAGTT+CGG + chr5_2:56672799-56672818 None:intergenic 45.0%
CGAGATAACCGTCAATAACG+TGG - chr5_2:56672884-56672903 Msa0777590:intron 45.0%
AATACTCGTCGGTCATCTGA+AGG + chr5_2:56672935-56672954 None:intergenic 45.0%
GTCGCCTTAACAACTCTTTC+CGG - chr5_2:56672999-56673018 Msa0777590:CDS 45.0%
ATTGCAGACCTTTGAGCTGA+CGG + chr5_2:56673105-56673124 None:intergenic 45.0%
TGTGTATGCAATCAACGCTC+CGG + chr5_2:56673255-56673274 None:intergenic 45.0%
TTTGATCACGTACAAGCTCC+CGG + chr5_2:56673289-56673308 None:intergenic 45.0%
GGAGCTTGTACGTGATCAAA+CGG - chr5_2:56673289-56673308 Msa0777590:CDS 45.0%
GAGCTTGTACGTGATCAAAC+GGG - chr5_2:56673290-56673309 Msa0777590:CDS 45.0%
! AGACAGCAGAGGAAGTTGAT+CGG + chr5_2:56673446-56673465 None:intergenic 45.0%
CACGTAGGTAAATAGCGCAA+CGG + chr5_2:56673472-56673491 None:intergenic 45.0%
GTTCTGTCATTCACCTACGA+CGG - chr5_2:56673569-56673588 Msa0777590:CDS 45.0%
! ATTTTCTCCGAACGTGCTAC+CGG + chr5_2:56673678-56673697 None:intergenic 45.0%
! TAGGTAAGCGTCCACAGTTT+TGG + chr5_2:56673726-56673745 None:intergenic 45.0%
CTAGCTGAGATTGCTTCCTA+CGG - chr5_2:56673743-56673762 Msa0777590:CDS 45.0%
!!! TGCGCCTTTTGGAATGAGAA+TGG + chr5_2:56673801-56673820 None:intergenic 45.0%
AACTTTGGTGAGAGAGAACG+AGG - chr5_2:56674295-56674314 Msa0777590:intron 45.0%
AGAGAACGAGGAGTTGAGAA+CGG - chr5_2:56674307-56674326 Msa0777590:intron 45.0%
! ACAACTTCATCGTCTGGAGT+TGG - chr5_2:56674386-56674405 Msa0777590:intron 45.0%
TGACACGGACGAAAAGAATG+TGG + chr5_2:56674429-56674448 None:intergenic 45.0%
TGACAAAGCCTAGAAGGAGA+AGG - chr5_2:56674525-56674544 Msa0777590:intron 45.0%
ATGGAGAACCTTCTCCTTCT+AGG + chr5_2:56674536-56674555 None:intergenic 45.0%
!! GTTTAGTATGGCGGGTTTGT+TGG - chr5_2:56674712-56674731 Msa0777590:five_prime_UTR 45.0%
ATGGCAAGAGAGAGTCTGCT+AGG + chr5_2:56672215-56672234 None:intergenic 50.0%
TAGCAGACTCTCTCTTGCCA+TGG - chr5_2:56672214-56672233 Msa0777590:three_prime_UTR 50.0%
TCTCTTGCCATGGAGAGAAC+AGG - chr5_2:56672224-56672243 Msa0777590:three_prime_UTR 50.0%
CTCTTGCCATGGAGAGAACA+GGG - chr5_2:56672225-56672244 Msa0777590:three_prime_UTR 50.0%
!! AGATGTGTTCGGTGTTCGAC+CGG + chr5_2:56672612-56672631 None:intergenic 50.0%
GATGTCGGAGAGGTCTAATC+TGG + chr5_2:56672808-56672827 None:intergenic 50.0%
CAACGACGCCACGTTATTGA+CGG + chr5_2:56672895-56672914 None:intergenic 50.0%
GTCGGTCATCTGAAGGAACA+CGG + chr5_2:56672928-56672947 None:intergenic 50.0%
! ATGACCGACGAGTATTGCGA+CGG - chr5_2:56672939-56672958 Msa0777590:intron 50.0%
GTTTCCGTCGCAATACTCGT+CGG + chr5_2:56672946-56672965 None:intergenic 50.0%
! AGCGCCGGAAAGAGTTGTTA+AGG + chr5_2:56673006-56673025 None:intergenic 50.0%
ATCACCGCCATGAAACAACG+TGG - chr5_2:56673209-56673228 Msa0777590:CDS 50.0%
! TTTCGCGCCACGTTGTTTCA+TGG + chr5_2:56673219-56673238 None:intergenic 50.0%
GCGAAATACCTAACTCTCGC+CGG - chr5_2:56673233-56673252 Msa0777590:CDS 50.0%
CATACACAGAACGCACACTC+CGG - chr5_2:56673267-56673286 Msa0777590:CDS 50.0%
ATACACAGAACGCACACTCC+GGG - chr5_2:56673268-56673287 Msa0777590:CDS 50.0%
CTTGTACGTGATCAAACGGG+TGG - chr5_2:56673293-56673312 Msa0777590:CDS 50.0%
GTGGCGGTGGAGGAAAATTT+CGG - chr5_2:56673321-56673340 Msa0777590:CDS 50.0%
CATCGTCTCTCTCTTTCCGT+CGG - chr5_2:56673406-56673425 Msa0777590:CDS 50.0%
GGAAAAGCAGCTTTCTCCGA+CGG + chr5_2:56673425-56673444 None:intergenic 50.0%
CGTAGGAAGCAATCTCAGCT+AGG + chr5_2:56673745-56673764 None:intergenic 50.0%
CGAAATGGTGAGTTCTCCGT+AGG + chr5_2:56673762-56673781 None:intergenic 50.0%
! TCGAGTTTTCGTGCGCCTTT+TGG + chr5_2:56673812-56673831 None:intergenic 50.0%
TGTGGCATGTGTGATGTTGC+AGG - chr5_2:56674058-56674077 Msa0777590:CDS 50.0%
! GGAGAAAGTGTGCAGTGTGA+TGG - chr5_2:56674109-56674128 Msa0777590:intron 50.0%
GCATGCAGTACAATCTGGAC+CGG + chr5_2:56674191-56674210 None:intergenic 50.0%
TTGCAAAAGAACGGTCACGG+CGG - chr5_2:56674362-56674381 Msa0777590:intron 50.0%
!! GTATGGCGGGTTTGTTGGTT+AGG - chr5_2:56674717-56674736 Msa0777590:five_prime_UTR 50.0%
CCATTGCCCTGTTCTCTCCA+TGG + chr5_2:56672234-56672253 None:intergenic 55.0%
CCATGGAGAGAACAGGGCAA+TGG - chr5_2:56672231-56672250 Msa0777590:three_prime_UTR 55.0%
TTAGACCTCTCCGACATCCC+CGG - chr5_2:56672810-56672829 Msa0777590:intron 55.0%
TCAAAAGCTTCTTGGCCGCC+GGG + chr5_2:56672831-56672850 None:intergenic 55.0%
CTCAAAAGCTTCTTGGCCGC+CGG + chr5_2:56672832-56672851 None:intergenic 55.0%
GGTCATCTGAAGGAACACGG+CGG + chr5_2:56672925-56672944 None:intergenic 55.0%
TATTGCGACGGAAACCTCGC+CGG - chr5_2:56672951-56672970 Msa0777590:intron 55.0%
AACCTCGCCGGAAGAACTGA+AGG - chr5_2:56672963-56672982 Msa0777590:intron 55.0%
AACCTTCAGTTCTTCCGGCG+AGG + chr5_2:56672968-56672987 None:intergenic 55.0%
!!! TTTTAGAACGACGGCAGCGC+CGG + chr5_2:56673021-56673040 None:intergenic 55.0%
! ATGTTTAGCTCGTCGGCGAC+GGG + chr5_2:56673062-56673081 None:intergenic 55.0%
! GATGTTTAGCTCGTCGGCGA+CGG + chr5_2:56673063-56673082 None:intergenic 55.0%
TGTTGACGCCGTCAGCTCAA+AGG - chr5_2:56673094-56673113 Msa0777590:CDS 55.0%
AATTCGGCGGTGATCGGCTA+GGG + chr5_2:56673139-56673158 None:intergenic 55.0%
TCCACCAATTCGGCGGTGAT+CGG + chr5_2:56673145-56673164 None:intergenic 55.0%
ATCACCGCCGAATTGGTGGA+CGG - chr5_2:56673145-56673164 Msa0777590:CDS 55.0%
AGCTCCTCCGTCCACCAATT+CGG + chr5_2:56673155-56673174 None:intergenic 55.0%
!! GGCGGTGATCACTTTTCCGA+AGG + chr5_2:56673198-56673217 None:intergenic 55.0%
GTACGTGATCAAACGGGTGG+CGG - chr5_2:56673296-56673315 Msa0777590:CDS 55.0%
CGACGGAAAGAGAGAGACGA+TGG + chr5_2:56673408-56673427 None:intergenic 55.0%
TTCACGCTTGCACGAGCTAG+AGG + chr5_2:56673495-56673514 None:intergenic 55.0%
CATTCACCTACGACGGTGAG+AGG - chr5_2:56673576-56673595 Msa0777590:CDS 55.0%
AGCACCACAGTGTTCAACGC+CGG - chr5_2:56673656-56673675 Msa0777590:CDS 55.0%
TTCAACGCCGGTAGCACGTT+CGG - chr5_2:56673668-56673687 Msa0777590:CDS 55.0%
!! AGTTTTGGCCACGCGTTGCA+TGG + chr5_2:56673711-56673730 None:intergenic 55.0%
CTCTTCCGTGTCACGTTCCA+GGG + chr5_2:56674252-56674271 None:intergenic 55.0%
TCTCTTCCGTGTCACGTTCC+AGG + chr5_2:56674253-56674272 None:intergenic 55.0%
TGGAACGTGACACGGAAGAG+AGG - chr5_2:56674252-56674271 Msa0777590:intron 55.0%
GGCGGAACAACTTCATCGTC+TGG - chr5_2:56674380-56674399 Msa0777590:intron 55.0%
CAAAAGCTTCTTGGCCGCCG+GGG + chr5_2:56672830-56672849 None:intergenic 60.0%
! TGTTTAGCTCGTCGGCGACG+GGG + chr5_2:56673061-56673080 None:intergenic 60.0%
CAATTCGGCGGTGATCGGCT+AGG + chr5_2:56673140-56673159 None:intergenic 60.0%
CTAGCCGATCACCGCCGAAT+TGG - chr5_2:56673138-56673157 Msa0777590:CDS 60.0%
TCCTCCGTCCACCAATTCGG+CGG + chr5_2:56673152-56673171 None:intergenic 60.0%
! CGCGCCACGTTGTTTCATGG+CGG + chr5_2:56673216-56673235 None:intergenic 60.0%
TCAACGCTCCGGCGAGAGTT+AGG + chr5_2:56673244-56673263 None:intergenic 60.0%
!! GATCAAACGGGTGGCGGAAG+TGG - chr5_2:56673302-56673321 Msa0777590:CDS 60.0%
CGCAACGGAGAAGACAGCAG+AGG + chr5_2:56673457-56673476 None:intergenic 60.0%
! TGCACGAGCTAGAGGCACGT+AGG + chr5_2:56673487-56673506 None:intergenic 60.0%
CAGAAGCCTCTCACCGTCGT+AGG + chr5_2:56673585-56673604 None:intergenic 60.0%
CTACGACGGTGAGAGGCTTC+TGG - chr5_2:56673583-56673602 Msa0777590:CDS 60.0%
! GCTACCGGCGTTGAACACTG+TGG + chr5_2:56673663-56673682 None:intergenic 60.0%
TTTGGCCACGCGTTGCATGG+CGG + chr5_2:56673708-56673727 None:intergenic 60.0%
GCAACGCGTGGCCAAAACTG+TGG - chr5_2:56673712-56673731 Msa0777590:CDS 60.0%
GATAAGAAGCGGGGCAACCC+TGG - chr5_2:56674232-56674251 Msa0777590:intron 60.0%
GGCAACCCTGGAACGTGACA+CGG - chr5_2:56674244-56674263 Msa0777590:intron 60.0%
TTCTTGGCCGCCGGGGATGT+CGG + chr5_2:56672823-56672842 None:intergenic 65.0%
GCCGATCACCGCCGAATTGG+TGG - chr5_2:56673141-56673160 Msa0777590:CDS 65.0%
! ACCGCCGAATTGGTGGACGG+AGG - chr5_2:56673148-56673167 Msa0777590:CDS 65.0%
!! CAAACGGGTGGCGGAAGTGG+CGG - chr5_2:56673305-56673324 Msa0777590:CDS 65.0%
GACCTCTCCGACATCCCCGG+CGG - chr5_2:56672813-56672832 Msa0777590:intron 70.0%
CGGCGACGGGGAGAAGCTGA+CGG + chr5_2:56673049-56673068 None:intergenic 70.0%
CTCTGCCGCCATGCAACGCG+TGG - chr5_2:56673700-56673719 Msa0777590:CDS 70.0%
!! ACGGGTGGCGGAAGTGGCGG+TGG - chr5_2:56673308-56673327 Msa0777590:CDS 75.0%
!! GGTGGCGGAAGTGGCGGTGG+AGG - chr5_2:56673311-56673330 Msa0777590:CDS 75.0%
! GGCCGCCGGGGATGTCGGAG+AGG + chr5_2:56672818-56672837 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 56671929 56674936 56671929 ID=Msa0777590;Name=Msa0777590
chr5_2 mRNA 56671929 56674936 56671929 ID=Msa0777590-mRNA-1;Parent=Msa0777590;Name=Msa0777590-mRNA-1;_AED=0.01;_eAED=0.01;_QI=268|1|1|1|1|1|4|374|579
chr5_2 exon 56671929 56672785 56671929 ID=Msa0777590-mRNA-1:exon:16754;Parent=Msa0777590-mRNA-1
chr5_2 exon 56672997 56674127 56672997 ID=Msa0777590-mRNA-1:exon:16753;Parent=Msa0777590-mRNA-1
chr5_2 exon 56674431 56674500 56674431 ID=Msa0777590-mRNA-1:exon:16752;Parent=Msa0777590-mRNA-1
chr5_2 exon 56674613 56674936 56674613 ID=Msa0777590-mRNA-1:exon:16751;Parent=Msa0777590-mRNA-1
chr5_2 five_prime_UTR 56674669 56674936 56674669 ID=Msa0777590-mRNA-1:five_prime_utr;Parent=Msa0777590-mRNA-1
chr5_2 CDS 56674613 56674668 56674613 ID=Msa0777590-mRNA-1:cds;Parent=Msa0777590-mRNA-1
chr5_2 CDS 56674431 56674500 56674431 ID=Msa0777590-mRNA-1:cds;Parent=Msa0777590-mRNA-1
chr5_2 CDS 56672997 56674127 56672997 ID=Msa0777590-mRNA-1:cds;Parent=Msa0777590-mRNA-1
chr5_2 CDS 56672303 56672785 56672303 ID=Msa0777590-mRNA-1:cds;Parent=Msa0777590-mRNA-1
chr5_2 three_prime_UTR 56671929 56672302 56671929 ID=Msa0777590-mRNA-1:three_prime_utr;Parent=Msa0777590-mRNA-1
Gene Sequence

>Msa0777590

ATGGAGAATCCTAATCCTAGCAGACTCTCTCTTGCCATGGAGAGAACAGGGCAATGGATTTTCTCTCAAGAAATCCCAAGTGATGTTATAGTTACAATTAGAGAAGCCACTTATTCTCTCCACAAGTTCATGCTGGTGGCAAAGAGCAACTACATCAGAAAGCTGATAATGGAATCAAACGAAACCGAACTCACCAGATTAGACCTCTCCGACATCCCCGGCGGCCAAGAAGCTTTTGAGAAAGCAGCTAAATTCTGTTACGGCGTCAACTTCGAGATAACCGTCAATAACGTGGCGTCGTTGCACTGCGCCGCCGTGTTCCTTCAGATGACCGACGAGTATTGCGACGGAAACCTCGCCGGAAGAACTGAAGGTTTTCTTTCTCAGGTCGCCTTAACAACTCTTTCCGGCGCTGCCGTCGTTCTAAAATCATGCCGTCAGCTTCTCCCCGTCGCCGACGAGCTAAACATCGTCAAACGCTGTGTTGACGCCGTCAGCTCAAAGGTCTGCAATGAAGCAAACTTCCCTAGCCGATCACCGCCGAATTGGTGGACGGAGGAGCTCGCCGTTCTTGACGTTGAATCCTTCGGAAAAGTGATCACCGCCATGAAACAACGTGGCGCGAAATACCTAACTCTCGCCGGAGCGTTGATTGCATACACAGAACGCACACTCCGGGAGCTTGTACGTGATCAAACGGGTGGCGGAAGTGGCGGTGGAGGAAAATTTCGGTCATCTGATTCAACAGATTCCGATTCAGAAACAAAATCCGAACAACGTGAGATTCTACAATCCATCGTCTCTCTCTTTCCGTCGGAGAAAGCTGCTTTTCCGATCAACTTCCTCTGCTGTCTTCTCCGTTGCGCTATTTACCTACGTGCCTCTAGCTCGTGCAAGCGTGAACTCGAGAAACGAATTGCTGAAATTCTCGAACACGTATCAGTGAACGATCTCTTAGTTCTGTCATTCACCTACGACGGTGAGAGGCTTCTGGATCTTGACAGCATTCGGAGAATCATTTCTGCATTCGTCGAAAAGGAGAAAAGCACCACAGTGTTCAACGCCGGTAGCACGTTCGGAGAAAATTGCTCTGCCGCCATGCAACGCGTGGCCAAAACTGTGGACGCTTACCTAGCTGAGATTGCTTCCTACGGAGAACTCACCATTTCGAAGTTCAACGGTATTGCCATTCTCATTCCAAAAGGCGCACGAAAACTCGACGATGATCTTTACCGTGCTGTCGATATTTATCTTAAGGTACACACCAATCTTGATGAGTTAGAGAAGGAGAAAGTGTGCAGTGTGATGGATGCACTTAAACTTTCATATGAGGCGCGTGTTCACGCGTCTCAGAACAAACGATTACCGGTCCAGATTGTACTGCATGCATTGTATTATGATCAATTAAAGATAAGAAGCGGGGCAACCCTGGAACGTGACACGGAAGAGAGGAATCAGTTACAAACAGACGTAACTTTGGTGAGAGAGAACGAGGAGTTGAGAACGGAGTTGATTAGGATGAAAATGTATGTTTCAGATTTGCAAAAGAACGGTCACGGCGGAACAACTTCATCGTCTGGAGTTGGAAAAGAAGTCAAAAAGTCCACATTCTTTTCGTCCGTGTCAAAGAAATTAGGTAAGCTGAATCCGTTCAAGAATGGTTCTAAAGATACGTCTCATATTGAGGATGTTGGTGTGGATTTGACAAAGCCTAGAAGGAGAAGGTTCTCCATTTCATAG
Protein sequence

>Msa0777590

MENPNPSRLSLAMERTGQWIFSQEIPSDVIVTIREATYSLHKFMLVAKSNYIRKLIMESNETELTRLDLSDIPGGQEAFEKAAKFCYGVNFEITVNNVASLHCAAVFLQMTDEYCDGNLAGRTEGFLSQVALTTLSGAAVVLKSCRQLLPVADELNIVKRCVDAVSSKVCNEANFPSRSPPNWWTEELAVLDVESFGKVITAMKQRGAKYLTLAGALIAYTERTLRELVRDQTGGGSGGGGKFRSSDSTDSDSETKSEQREILQSIVSLFPSEKAAFPINFLCCLLRCAIYLRASSSCKRELEKRIAEILEHVSVNDLLVLSFTYDGERLLDLDSIRRIISAFVEKEKSTTVFNAGSTFGENCSAAMQRVAKTVDAYLAEIASYGELTISKFNGIAILIPKGARKLDDDLYRAVDIYLKVHTNLDELEKEKVCSVMDALKLSYEARVHASQNKRLPVQIVLHALYYDQLKIRSGATLERDTEERNQLQTDVTLVRENEELRTELIRMKMYVSDLQKNGHGGTTSSSGVGKEVKKSTFFSSVSKKLGKLNPFKNGSKDTSHIEDVGVDLTKPRRRRFSIS*