AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0814650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0814650 MtrunA17_Chr5g0432861 98.446 579 9 0 1 579 1 579 0.0 1177
Msa0814650 MtrunA17_Chr3g0108331 72.586 580 139 8 5 579 4 568 0.0 847
Msa0814650 MtrunA17_Chr5g0401451 39.430 596 295 7 9 551 24 606 1.23e-147 439
Msa0814650 MtrunA17_Chr4g0053841 37.561 615 316 11 13 579 1 595 1.73e-137 412
Msa0814650 MtrunA17_Chr4g0010091 36.519 586 313 8 9 551 16 585 7.43e-127 385
Msa0814650 MtrunA17_Chr1g0195911 34.066 637 320 15 8 579 20 621 2.01e-111 346
Msa0814650 MtrunA17_Chr7g0245201 34.003 597 344 12 20 579 57 640 9.88e-108 337
Msa0814650 MtrunA17_Chr2g0303541 34.242 587 330 8 12 546 12 594 2.91e-106 333
Msa0814650 MtrunA17_Chr7g0268781 33.775 604 309 11 8 549 20 594 1.06e-105 331
Msa0814650 MtrunA17_Chr2g0279481 32.073 661 348 13 13 579 32 685 1.88e-103 327
Msa0814650 MtrunA17_Chr2g0285511 35.119 504 279 7 12 477 12 505 7.92e-101 318
Msa0814650 MtrunA17_Chr4g0050021 34.570 512 279 9 14 471 33 542 5.30e-98 312
Msa0814650 MtrunA17_Chr8g0374011 34.039 661 313 18 17 574 11 651 3.14e-96 307
Msa0814650 MtrunA17_Chr1g0181181 35.000 480 278 7 19 477 20 486 6.38e-95 301
Msa0814650 MtrunA17_Chr2g0320691 34.574 564 311 11 6 516 4 562 1.85e-94 302
Msa0814650 MtrunA17_Chr8g0382231 32.630 521 312 8 25 517 52 561 5.12e-93 298
Msa0814650 MtrunA17_Chr1g0199631 32.364 550 309 13 43 546 8 540 3.13e-83 271
Msa0814650 MtrunA17_Chr4g0076521 32.334 467 286 4 21 470 22 475 8.17e-80 263
Msa0814650 MtrunA17_Chr1g0207461 31.161 491 281 8 22 477 25 493 4.98e-77 256
Msa0814650 MtrunA17_Chr5g0412591 34.568 486 274 9 21 475 22 494 4.34e-75 250
Msa0814650 MtrunA17_Chr1g0191711 27.757 526 333 9 12 517 44 542 2.07e-73 244
Msa0814650 MtrunA17_Chr1g0150191 30.303 495 289 9 12 469 12 487 9.44e-73 244
Msa0814650 MtrunA17_Chr5g0401341 31.875 480 272 9 24 470 26 483 1.45e-72 244
Msa0814650 MtrunA17_Chr8g0367891 33.265 487 260 10 28 475 29 489 3.25e-72 242
Msa0814650 MtrunA17_Chr2g0290741 30.172 464 284 5 24 470 25 465 3.94e-69 233
Msa0814650 MtrunA17_Chr4g0003891 31.250 304 186 4 172 471 30 314 5.36e-44 162
Msa0814650 MtrunA17_Chr2g0332281 31.250 288 181 2 184 471 24 294 1.68e-42 157
Msa0814650 MtrunA17_Chr4g0037661 26.866 469 258 10 69 487 51 484 4.26e-41 156
Msa0814650 MtrunA17_Chr1g0206621 46.622 148 73 3 33 178 76 219 1.31e-38 142
Msa0814650 MtrunA17_Chr1g0163311 25.184 544 299 15 35 516 14 511 8.12e-36 140
Msa0814650 MtrunA17_Chr4g0023801 24.119 539 313 14 35 516 15 514 2.30e-32 131
Msa0814650 MtrunA17_Chr2g0280541 25.815 399 220 12 92 456 2 358 1.26e-24 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0814650 AT2G30520.1 67.003 594 174 6 2 579 6 593 0.0 810
Msa0814650 AT2G30520.2 67.877 551 156 5 44 579 1 545 0.0 756
Msa0814650 AT2G30520.3 67.877 551 156 5 44 579 1 545 0.0 756
Msa0814650 AT5G67385.1 38.746 622 304 12 9 579 9 604 4.64e-138 414
Msa0814650 AT5G48800.1 36.439 601 285 11 8 551 22 582 1.63e-121 372
Msa0814650 AT5G67385.4 37.265 585 290 12 46 579 1 559 8.14e-121 368
Msa0814650 AT5G67385.5 37.265 585 290 12 46 579 1 559 8.14e-121 368
Msa0814650 AT5G67385.2 37.265 585 290 12 46 579 1 559 8.14e-121 368
Msa0814650 AT5G67385.3 37.265 585 290 12 46 579 1 559 8.14e-121 368
Msa0814650 AT1G30440.1 33.333 648 340 12 12 575 12 651 2.15e-107 337
Msa0814650 AT3G08570.1 34.247 584 309 14 20 554 30 587 1.50e-105 331
Msa0814650 AT1G03010.1 33.605 613 305 14 8 549 20 601 6.54e-105 330
Msa0814650 AT1G03010.2 33.836 597 294 13 24 549 8 574 1.59e-103 325
Msa0814650 AT1G03010.3 33.836 597 294 13 24 549 34 600 4.56e-103 325
Msa0814650 AT2G47860.1 32.718 596 325 9 8 549 21 594 7.92e-103 324
Msa0814650 AT3G49970.1 34.991 563 301 14 33 579 2 515 1.14e-99 312
Msa0814650 AT3G49970.2 34.991 563 301 14 33 579 2 515 1.14e-99 312
Msa0814650 AT2G47860.3 31.502 619 325 10 8 549 21 617 1.01e-97 311
Msa0814650 AT5G64330.2 32.419 583 281 8 15 487 42 621 2.39e-95 305
Msa0814650 AT5G64330.1 32.419 583 281 8 15 487 42 621 1.21e-94 306
Msa0814650 AT5G13600.1 32.218 568 351 8 19 557 20 582 9.34e-94 299
Msa0814650 AT3G08660.1 32.743 565 310 16 20 551 30 557 8.77e-93 296
Msa0814650 AT5G03250.1 32.617 512 289 8 10 477 10 509 7.74e-90 289
Msa0814650 AT5G64330.3 31.835 556 266 8 42 487 10 562 3.45e-86 280
Msa0814650 AT3G08660.3 32.270 533 292 15 52 551 1 497 5.96e-85 274
Msa0814650 AT3G44820.1 30.893 560 321 10 19 516 17 572 1.05e-84 277
Msa0814650 AT3G50840.1 33.895 534 301 11 11 516 1 510 1.67e-82 269
Msa0814650 AT5G66560.1 32.402 537 268 9 17 472 10 532 1.91e-82 271
Msa0814650 AT5G10250.2 32.541 547 319 12 6 517 27 558 2.13e-82 270
Msa0814650 AT5G10250.1 33.210 539 311 13 6 517 27 543 8.05e-81 266
Msa0814650 AT2G47860.4 34.305 446 238 8 8 420 21 444 2.88e-80 259
Msa0814650 AT5G66560.3 32.558 516 254 8 37 472 2 503 3.13e-78 259
Msa0814650 AT5G66560.4 34.842 442 238 6 67 472 66 493 1.25e-77 258
Msa0814650 AT2G47860.6 32.623 469 238 9 8 420 21 467 4.85e-75 246
Msa0814650 AT3G50840.2 33.936 498 278 10 46 516 1 474 1.33e-73 244
Msa0814650 AT3G50840.3 33.936 498 278 10 46 516 1 474 1.33e-73 244
Msa0814650 AT5G47800.3 30.228 483 304 5 20 483 21 489 6.76e-73 243
Msa0814650 AT5G47800.1 30.228 483 304 5 20 483 21 489 6.76e-73 243
Msa0814650 AT5G47800.4 30.228 483 304 5 20 483 21 489 6.76e-73 243
Msa0814650 AT1G67900.5 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT1G67900.1 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT1G67900.3 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT1G67900.6 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT1G67900.2 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT1G67900.4 33.269 517 287 7 22 491 23 528 1.56e-72 244
Msa0814650 AT5G47800.2 30.228 483 304 5 20 483 134 602 1.11e-71 243
Msa0814650 AT4G31820.1 28.967 542 311 9 24 535 26 523 1.25e-71 240
Msa0814650 AT2G47860.5 29.960 494 274 7 110 549 1 476 3.52e-69 233
Msa0814650 AT2G47860.2 29.960 494 274 7 110 549 1 476 3.52e-69 233
Msa0814650 AT3G08660.2 31.667 420 241 11 20 423 30 419 1.76e-67 225
Msa0814650 AT2G14820.2 29.878 492 283 9 24 477 26 493 5.09e-67 229
Msa0814650 AT2G14820.1 29.878 492 283 9 24 477 26 493 5.09e-67 229
Msa0814650 AT5G67440.3 30.867 473 279 9 24 470 26 476 1.18e-65 224
Msa0814650 AT5G67440.1 30.867 473 279 9 24 470 26 476 1.18e-65 224
Msa0814650 AT5G66560.2 30.928 485 240 9 17 420 10 480 1.17e-63 217
Msa0814650 AT2G23050.1 30.538 465 251 10 38 477 40 457 1.77e-63 216
Msa0814650 AT4G31820.2 28.016 514 296 9 52 535 1 470 4.63e-62 213
Msa0814650 AT5G03250.2 30.318 409 232 6 110 477 1 397 4.84e-62 213
Msa0814650 AT5G67440.2 30.635 457 269 9 40 470 5 439 6.74e-61 211
Msa0814650 AT4G37590.1 29.135 532 294 11 22 509 24 516 9.96e-61 211
Msa0814650 AT3G26490.1 27.767 533 294 10 22 494 23 524 1.70e-58 206
Msa0814650 AT3G26490.3 27.767 533 294 10 22 494 23 524 1.70e-58 206
Msa0814650 AT3G26490.4 27.767 533 294 10 22 494 23 524 1.70e-58 206
Msa0814650 AT5G48130.1 27.423 485 269 14 19 469 33 468 2.53e-45 170
Msa0814650 AT3G26490.2 27.489 462 251 9 22 423 23 460 3.63e-45 167
Msa0814650 AT3G19850.1 25.299 502 292 8 35 488 14 480 1.15e-43 164
Msa0814650 AT1G52770.1 31.250 288 187 3 184 471 28 304 4.92e-42 157
Msa0814650 AT2G23050.2 27.835 388 213 8 110 477 1 341 4.07e-41 153
Msa0814650 AT3G15570.1 32.667 300 171 6 184 471 29 309 7.45e-41 154
Msa0814650 AT1G50280.1 26.096 479 273 10 35 464 14 460 3.72e-38 148
Msa0814650 AT1G50280.2 26.697 442 248 9 67 464 18 427 1.04e-37 146
Msa0814650 AT3G03510.1 25.105 474 299 11 79 546 8 431 8.23e-35 137
Msa0814650 AT5G17580.2 24.792 480 297 9 27 478 22 465 5.18e-33 133
Msa0814650 AT5G17580.1 24.792 480 297 9 27 478 7 450 7.25e-33 133
Msa0814650 AT3G22104.1 26.667 465 255 13 27 455 6 420 3.32e-32 130
Msa0814650 AT3G22104.4 25.191 393 211 10 96 455 3 345 1.22e-22 101
Msa0814650 AT3G22104.2 25.191 393 211 10 96 455 3 345 1.22e-22 101
Msa0814650 AT3G22104.3 25.191 393 211 10 96 455 3 345 1.22e-22 101
Msa0814650 AT3G49900.2 29.464 224 130 9 10 222 49 255 6.82e-13 71.6
Msa0814650 AT3G49900.1 28.632 234 139 9 10 232 49 265 6.96e-13 71.6

Find 163 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGAACCTTCTCCTTCT+AGG 0.245811 5_3:+46899918 None:intergenic
CTGCTTTCTCAAAAGCTTCT+TGG 0.250098 5_3:+46901606 None:intergenic
CTATAAAATGCAATTTGTTT+GGG 0.268314 5_3:-46899779 Msa0814650:three_prime_UTR
AACAGTAGTACTAGTTTATA+TGG 0.271753 5_3:+46902332 None:intergenic
TCGTCCGTGTCAAAGAAATT+AGG 0.274325 5_3:-46900014 Msa0814650:CDS
ACTATAAAATGCAATTTGTT+TGG 0.275295 5_3:-46899780 Msa0814650:three_prime_UTR
GTATGGCGGGTTTGTTGATT+AGG 0.296396 5_3:-46899734 Msa0814650:three_prime_UTR
CTACGACGGCGAGAGGCTTC+TGG 0.304375 5_3:-46900859 Msa0814650:CDS
AGTTGAGAACGGAGTTGATT+AGG 0.340136 5_3:-46900133 Msa0814650:CDS
CTACATCAGAAAACTGATAA+TGG 0.340371 5_3:-46901681 Msa0814650:CDS
TGCTGTCGATATTTATCTTA+AGG 0.341842 5_3:-46900595 Msa0814650:intron
AGTACTAGTTTATATGGTTA+TGG 0.354211 5_3:+46902338 None:intergenic
TCAACGCTCCGGCGAGAGTT+AGG 0.356655 5_3:+46901201 None:intergenic
TTATGGTGGATTGTTGTAAT+AGG 0.365816 5_3:+46902355 None:intergenic
GAGAAAACCTTCAGTTCTTC+CGG 0.376275 5_3:+46901472 None:intergenic
TTCTGGATCTTGACAGCATT+CGG 0.378888 5_3:-46900842 Msa0814650:CDS
GTGGCCTTAACAACTCTTTC+CGG 0.381358 5_3:-46901443 Msa0814650:CDS
TAGGTAAGCGTCCACAGTTT+TGG 0.383838 5_3:+46900719 None:intergenic
TTGTAGAATCTCACGTTGTT+CGG 0.393991 5_3:+46901061 None:intergenic
CTCAAAAGCTTCTTGGCCGC+CGG 0.395497 5_3:+46901613 None:intergenic
ATCTCAGAACAAACGATTAC+CGG 0.398634 5_3:-46900282 Msa0814650:CDS
AAACAAAATAATCTATGAAA+TGG 0.401564 5_3:+46899899 None:intergenic
AACATCATCATAGTTTAGTA+TGG 0.413333 5_3:-46899751 Msa0814650:three_prime_UTR
TCTCTTGCCATGGAGAGAAC+AGG 0.413490 5_3:-46902228 Msa0814650:CDS
AGAGAGAGTCTGCTAGGATT+AGG 0.417337 5_3:+46902246 None:intergenic
AACATATCAACTTTGTGTCT+TGG 0.420123 5_3:-46902461 Msa0814650:five_prime_UTR
ACCGGTGGCGGAAGTGGTGG+TGG 0.420130 5_3:-46901134 Msa0814650:CDS
TGGATTTGACAAAGCCTAGA+AGG 0.433974 5_3:-46899932 Msa0814650:CDS
TCCACCACCACTTCCGCCAC+CGG 0.436475 5_3:+46901133 None:intergenic
AAAACAATAACATAAATTCA+TGG 0.436908 5_3:-46902274 Msa0814650:exon
CCATTGCCCTGTTCTCTCCA+TGG 0.437876 5_3:+46902221 None:intergenic
CTAGCCGATCACCGCCGAAT+TGG 0.439522 5_3:-46901304 Msa0814650:CDS
AGCTCCTCCGTCCACCAATT+CGG 0.447590 5_3:+46901290 None:intergenic
TCAAAAGCTTCTTGGCCGCC+GGG 0.451799 5_3:+46901614 None:intergenic
TTCTTGGCCGCCGGGGATGT+CGG 0.452645 5_3:+46901622 None:intergenic
GTTCTTGACGTTGAATCCTT+CGG 0.454031 5_3:-46901263 Msa0814650:CDS
GATCAAACCGGTGGCGGAAG+TGG 0.455777 5_3:-46901140 Msa0814650:CDS
GCCGATCACCGCCGAATTGG+TGG 0.462783 5_3:-46901301 Msa0814650:CDS
ACGGCAATTATTAAGTGAAA+AGG 0.465717 5_3:-46902485 Msa0814650:five_prime_UTR
TCCACCAATTCGGCGGTGAT+CGG 0.469096 5_3:+46901300 None:intergenic
ATTACAAACAGACGTAACTT+TGG 0.470393 5_3:-46900171 Msa0814650:CDS
GAGGTCTAATCTGGTGAGTT+CGG 0.471565 5_3:+46901646 None:intergenic
TCTCCTTCTCTAACTCATCA+AGG 0.471656 5_3:+46900360 None:intergenic
TTCAACGCCGGTACCACGTT+CGG 0.474403 5_3:-46900774 Msa0814650:CDS
GGCGGAACAACTTCATCGTC+TGG 0.474475 5_3:-46900071 Msa0814650:CDS
TGCACTAAAACTTTCATATG+AGG 0.474878 5_3:-46900318 Msa0814650:CDS
CGTTGACGCCGTTAGCTCAA+AGG 0.476106 5_3:-46901348 Msa0814650:CDS
TGTGTACGTATATATACGTT+TGG 0.476284 5_3:-46899639 Msa0814650:three_prime_UTR
ATGTTTAGTTCATCGGCGAC+TGG 0.479934 5_3:+46901383 None:intergenic
CATCTCCGCATTCGTCGAAA+AGG 0.480627 5_3:-46900814 Msa0814650:CDS
TACCTTGTGGAGAGAATAAG+TGG 0.481583 5_3:+46902037 None:intergenic
GATGTCGGAGAGGTCTAATC+TGG 0.483299 5_3:+46901637 None:intergenic
GGTGGCGGAAGTGGTGGTGG+AGG 0.484410 5_3:-46901131 Msa0814650:CDS
TTTGGTCTGAAATTGTTATG+TGG 0.497692 5_3:+46902300 None:intergenic
TAACAATGAAGTTAATCCAC+TGG 0.499013 5_3:-46902390 Msa0814650:five_prime_UTR
TCATCATAGTTTAGTATGGC+GGG 0.499235 5_3:-46899747 Msa0814650:three_prime_UTR
CTCTGCCGCCATGCAACGCG+TGG 0.499587 5_3:-46900742 Msa0814650:CDS
CAACGACGCAACGTTGTTGA+CGG 0.499873 5_3:+46901550 None:intergenic
ACTAGTTTATATGGTTATGG+TGG 0.500487 5_3:+46902341 None:intergenic
GGAGAAAGTGTGCAGTGTGA+TGG 0.507183 5_3:-46900342 Msa0814650:CDS
GTCTTTGTATCAAAGAAGAA+AGG 0.507825 5_3:-46899663 Msa0814650:three_prime_UTR
ACAACTTCATCGTCTGGAGT+TGG 0.509583 5_3:-46900065 Msa0814650:CDS
CATACACAGAACGCACGCTC+CGG 0.509693 5_3:-46901175 Msa0814650:CDS
TAAAGATACGTCTCATATTG+AGG 0.512243 5_3:-46899964 Msa0814650:CDS
TATTGCGACGGAAACCTTGC+CGG 0.514015 5_3:-46901491 Msa0814650:CDS
ATTGCAAACCTTTGAGCTAA+CGG 0.514304 5_3:+46901340 None:intergenic
GTGGTGGTGGAGGAAAATTC+CGG 0.514703 5_3:-46901121 Msa0814650:CDS
CTTTAGAACGACGGCGGCGC+CGG 0.516206 5_3:+46901424 None:intergenic
GGCGCCGGAAAGAGTTGTTA+AGG 0.517467 5_3:+46901439 None:intergenic
ATCAATTAAAGATAAGAAGT+GGG 0.518668 5_3:-46900229 Msa0814650:CDS
ATACACAGAACGCACGCTCC+GGG 0.523598 5_3:-46901174 Msa0814650:CDS
AACCTTCAGTTCTTCCGGCA+AGG 0.524836 5_3:+46901477 None:intergenic
AAATGCAATTTGTTTGGGGT+AGG 0.531408 5_3:-46899774 Msa0814650:three_prime_UTR
TGTTTAGTTCATCGGCGACT+GGG 0.533764 5_3:+46901384 None:intergenic
TTTGACGATGTTTAGTTCAT+CGG 0.533777 5_3:+46901376 None:intergenic
CTTTACTACAAGCAAGATAA+CGG 0.536104 5_3:-46902504 Msa0814650:five_prime_UTR
ATTGCCATTCTCATTCCAAA+AGG 0.536892 5_3:-46900648 Msa0814650:CDS
AATTCGGCGGTGATCGGCTA+GGG 0.539024 5_3:+46901306 None:intergenic
AAAGCAGCTAAATTCTGTTA+CGG 0.541352 5_3:-46901590 Msa0814650:CDS
CTTCTCTAACTCATCAAGGT+TGG 0.541918 5_3:+46900364 None:intergenic
AACAATGAAGTTAATCCACT+GGG 0.542253 5_3:-46902389 Msa0814650:five_prime_UTR
GTTTCAGATTTGCAAAAGAA+CGG 0.543872 5_3:-46900098 Msa0814650:CDS
AATACCGTTGAACTTCGAAA+TGG 0.548610 5_3:+46900668 None:intergenic
CAATTCGGCGGTGATCGGCT+AGG 0.550046 5_3:+46901305 None:intergenic
GATCAATTAAAGATAAGAAG+TGG 0.550442 5_3:-46900230 Msa0814650:CDS
AAGCTGAATCCGTTCAAGAA+TGG 0.550799 5_3:-46899990 Msa0814650:CDS
CAAACCGGTGGCGGAAGTGG+TGG 0.552994 5_3:-46901137 Msa0814650:CDS
AACCTTGCCGGAAGAACTGA+AGG 0.554667 5_3:-46901479 Msa0814650:CDS
GAAGGATTCAACGTCAAGAA+CGG 0.559469 5_3:+46901265 None:intergenic
TTTGATCACGTACAAGCTCC+CGG 0.560599 5_3:+46901156 None:intergenic
CGTAGGAAGCAATCTCAGCT+AGG 0.562734 5_3:+46900700 None:intergenic
TCTTTAGAACCATTCTTGAA+CGG 0.565852 5_3:+46899981 None:intergenic
GAGCTTGTACGTGATCAAAC+CGG 0.567341 5_3:-46901152 Msa0814650:CDS
AGACAGCAGAGGAAGTTGAT+CGG 0.571060 5_3:+46900999 None:intergenic
CGCGCCACGTTGTTTCATTG+CGG 0.572972 5_3:+46901229 None:intergenic
CTCTTGCCATGGAGAGAACA+GGG 0.575153 5_3:-46902227 Msa0814650:CDS
TGTGTATGCAATCAACGCTC+CGG 0.576129 5_3:+46901190 None:intergenic
CATCGTCTCTCTCTTTCCAT+CGG 0.577539 5_3:-46901036 Msa0814650:CDS
AATACTCATCGGTCATCTGA+AGG 0.578324 5_3:+46901510 None:intergenic
TAGCAGACTCTCTCTTGCCA+TGG 0.578808 5_3:-46902238 Msa0814650:CDS
TGACAAAGCCTAGAAGGAGA+AGG 0.583766 5_3:-46899926 Msa0814650:CDS
GGAAAAGCAGCTTTCTCCGA+TGG 0.584047 5_3:+46901020 None:intergenic
AATCTGTTGAATCAGATGAC+CGG 0.584338 5_3:+46901102 None:intergenic
CAGAAGCCTCTCGCCGTCGT+AGG 0.585100 5_3:+46900860 None:intergenic
CTTACCTAATTTCTTTGACA+CGG 0.586200 5_3:+46900010 None:intergenic
GTTTCCGTCGCAATACTCAT+CGG 0.586697 5_3:+46901499 None:intergenic
ATGGCAAGAGAGAGTCTGCT+AGG 0.588044 5_3:+46902240 None:intergenic
TATAAAATGCAATTTGTTTG+GGG 0.589000 5_3:-46899778 Msa0814650:three_prime_UTR
CTAGCTGAGATTGCTTCCTA+CGG 0.589130 5_3:-46900699 Msa0814650:CDS
ACGTCTCATATTGAGGATGT+TGG 0.590809 5_3:-46899957 Msa0814650:CDS
GCGAAATACCTAACTCTCGC+CGG 0.591461 5_3:-46901209 Msa0814650:CDS
TTAGACCTCTCCGACATCCC+CGG 0.592064 5_3:-46901632 Msa0814650:CDS
CAGAACGTGACGCAGAAGAG+AGG 0.593103 5_3:-46900199 Msa0814650:CDS
CGAAATGGTGAGTTCTCCGT+AGG 0.593883 5_3:+46900683 None:intergenic
TTCACGCTTGCACGAGCTAG+AGG 0.595491 5_3:+46900950 None:intergenic
GTACGTATATATACGTTTGG+AGG 0.599614 5_3:-46899636 Msa0814650:three_prime_UTR
GTACGTGATCAAACCGGTGG+CGG 0.600315 5_3:-46901146 Msa0814650:CDS
CGGCGACTGGGAGAAGCTGA+CGG 0.601961 5_3:+46901396 None:intergenic
GATTTGCAAAAGAACGGTCA+CGG 0.603295 5_3:-46900092 Msa0814650:CDS
CTCACCATTTCGAAGTTCAA+CGG 0.607753 5_3:-46900672 Msa0814650:CDS
ATCATCATAGTTTAGTATGG+CGG 0.608805 5_3:-46899748 Msa0814650:three_prime_UTR
AGAGAACGAGGAGTTGAGAA+CGG 0.616083 5_3:-46900144 Msa0814650:CDS
TCCTCCGTCCACCAATTCGG+CGG 0.617815 5_3:+46901293 None:intergenic
AGCACCACGGTGTTCAACGC+CGG 0.618615 5_3:-46900786 Msa0814650:CDS
CGATGGAAAGAGAGAGACGA+TGG 0.618749 5_3:+46901037 None:intergenic
CAACATGTAACAGTTCATGC+TGG 0.620723 5_3:-46901717 Msa0814650:intron
GCAACGCGTGGCCAAAACTG+TGG 0.622071 5_3:-46900730 Msa0814650:CDS
GGCCGCCGGGGATGTCGGAG+AGG 0.623886 5_3:+46901627 None:intergenic
ATTGTAACTAGAACATCACT+TGG 0.626722 5_3:+46902068 None:intergenic
CCATGGAGAGAACAGGGCAA+TGG 0.627320 5_3:-46902221 Msa0814650:intron
CAACCTTGATGAGTTAGAGA+AGG 0.629964 5_3:-46900363 Msa0814650:CDS
ATCACCGCCGAATTGGTGGA+CGG 0.634104 5_3:-46901297 Msa0814650:CDS
CAAAAGCTTCTTGGCCGCCG+GGG 0.637469 5_3:+46901615 None:intergenic
TTTGGCCACGCGTTGCATGG+CGG 0.640820 5_3:+46900737 None:intergenic
TCATATTGAGGATGTTGGTG+TGG 0.644673 5_3:-46899952 Msa0814650:CDS
CATGTAACAGTTCATGCTGG+TGG 0.652427 5_3:-46901714 Msa0814650:intron
TCAATTAAAGATAAGAAGTG+GGG 0.656602 5_3:-46900228 Msa0814650:CDS
TTGTAACTAGAACATCACTT+GGG 0.656800 5_3:+46902069 None:intergenic
ATCGGTCATCTGAAGGAACA+CGG 0.660840 5_3:+46901517 None:intergenic
TAAGATAAATATCGACAGCA+CGG 0.663459 5_3:+46900598 None:intergenic
CATTCACCTACGACGGCGAG+AGG 0.670090 5_3:-46900866 Msa0814650:CDS
AACTTTGGTGAGAGAGAACG+AGG 0.672959 5_3:-46900156 Msa0814650:CDS
ATGACCGATGAGTATTGCGA+CGG 0.673009 5_3:-46901503 Msa0814650:CDS
AGCCACTTATTCTCTCCACA+AGG 0.673761 5_3:-46902039 Msa0814650:intron
CACGTAGGTAAATAGCGCAA+CGG 0.677467 5_3:+46900973 None:intergenic
GCGTGTAGCACAATCTGCAC+CGG 0.678026 5_3:+46900263 None:intergenic
CGCAACGGAGAAGACAGCAG+AGG 0.680863 5_3:+46900988 None:intergenic
TGACACGGACGAAAAGAATG+TGG 0.683709 5_3:+46900025 None:intergenic
GTTCTGTCATTCACCTACGA+CGG 0.685821 5_3:-46900873 Msa0814650:CDS
ACCGCCGAATTGGTGGACGG+AGG 0.689811 5_3:-46901294 Msa0814650:CDS
TTGCAAAAGAACGGTCACGG+CGG 0.698644 5_3:-46900089 Msa0814650:CDS
CTTGTACGTGATCAAACCGG+TGG 0.702310 5_3:-46901149 Msa0814650:CDS
TAGGTAATGTAGAACCCCAG+TGG 0.711562 5_3:+46902374 None:intergenic
ACGGCATGACTTTAGAACGA+CGG 0.714547 5_3:+46901415 None:intergenic
GCATGACTTTAGAACGACGG+CGG 0.721632 5_3:+46901418 None:intergenic
GACCTCTCCGACATCCCCGG+CGG 0.736635 5_3:-46901629 Msa0814650:CDS
GACATGTAAGTTTGTCACTG+TGG 0.753540 5_3:+46902437 None:intergenic
GGTACCGGCGTTGAACACCG+TGG 0.755346 5_3:+46900782 None:intergenic
TGCACGAGCTAGAGGCACGT+AGG 0.757887 5_3:+46900958 None:intergenic
CGAAAAGGAGAAAAGCACCA+CGG 0.762081 5_3:-46900799 Msa0814650:CDS
ACAATGAAGTTAATCCACTG+GGG 0.781409 5_3:-46902388 Msa0814650:five_prime_UTR
ATCACCGCAATGAAACAACG+TGG 0.797430 5_3:-46901233 Msa0814650:CDS
GGTCATCTGAAGGAACACGG+CGG 0.839358 5_3:+46901520 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTTATTGTTTTTCTTT+TGG + chr5_3:46899777-46899796 None:intergenic 15.0%
!! AAAACAATAACATAAATTCA+TGG - chr5_3:46899782-46899801 Msa0814650:three_prime_UTR 15.0%
!!! TTTTATGTATAAGATAAAGT+TGG + chr5_3:46901606-46901625 None:intergenic 15.0%
!! AAACAAAATAATCTATGAAA+TGG + chr5_3:46902160-46902179 None:intergenic 15.0%
!! TAAAAACTATTGTCATACTA+TGG + chr5_3:46901642-46901661 None:intergenic 20.0%
!! ATCAATTAAAGATAAGAAGT+GGG - chr5_3:46901827-46901846 Msa0814650:intron 20.0%
!!! ACTATAAAATGCAATTTGTT+TGG - chr5_3:46902276-46902295 Msa0814650:exon 20.0%
!!! CTATAAAATGCAATTTGTTT+GGG - chr5_3:46902277-46902296 Msa0814650:exon 20.0%
!!! TATAAAATGCAATTTGTTTG+GGG - chr5_3:46902278-46902297 Msa0814650:five_prime_UTR 20.0%
!! AGTACTAGTTTATATGGTTA+TGG + chr5_3:46899721-46899740 None:intergenic 25.0%
!! AACAGTAGTACTAGTTTATA+TGG + chr5_3:46899727-46899746 None:intergenic 25.0%
! CTTGAAACTAAAACAACTAT+GGG + chr5_3:46899906-46899925 None:intergenic 25.0%
! ACTTGAAACTAAAACAACTA+TGG + chr5_3:46899907-46899926 None:intergenic 25.0%
!! TAGTAGTTTTTAATACCTTG+TGG + chr5_3:46900035-46900054 None:intergenic 25.0%
! TATTGTCATACTATGGTTAA+TGG + chr5_3:46901635-46901654 None:intergenic 25.0%
!! TTTTAACATAACATGTGTGA+TGG - chr5_3:46901657-46901676 Msa0814650:CDS 25.0%
! GATCAATTAAAGATAAGAAG+TGG - chr5_3:46901826-46901845 Msa0814650:intron 25.0%
! TCAATTAAAGATAAGAAGTG+GGG - chr5_3:46901828-46901847 Msa0814650:intron 25.0%
! AACATCATCATAGTTTAGTA+TGG - chr5_3:46902305-46902324 Msa0814650:five_prime_UTR 25.0%
CTTTACTACAAGCAAGATAA+CGG - chr5_3:46899552-46899571 Msa0814650:three_prime_UTR 30.0%
ACGGCAATTATTAAGTGAAA+AGG - chr5_3:46899571-46899590 Msa0814650:three_prime_UTR 30.0%
AACATATCAACTTTGTGTCT+TGG - chr5_3:46899595-46899614 Msa0814650:three_prime_UTR 30.0%
TAACAATGAAGTTAATCCAC+TGG - chr5_3:46899666-46899685 Msa0814650:three_prime_UTR 30.0%
AACAATGAAGTTAATCCACT+GGG - chr5_3:46899667-46899686 Msa0814650:three_prime_UTR 30.0%
!! TTATGGTGGATTGTTGTAAT+AGG + chr5_3:46899704-46899723 None:intergenic 30.0%
! ACTAGTTTATATGGTTATGG+TGG + chr5_3:46899718-46899737 None:intergenic 30.0%
! TTTGGTCTGAAATTGTTATG+TGG + chr5_3:46899759-46899778 None:intergenic 30.0%
! TTTGTTGCTTGCATTATTTC+AGG - chr5_3:46899947-46899966 Msa0814650:CDS 30.0%
TTGTAACTAGAACATCACTT+GGG + chr5_3:46899990-46900009 None:intergenic 30.0%
ATTGTAACTAGAACATCACT+TGG + chr5_3:46899991-46900010 None:intergenic 30.0%
CATCTGATTAAAGATGTGTT+CGG + chr5_3:46900227-46900246 None:intergenic 30.0%
CTACATCAGAAAACTGATAA+TGG - chr5_3:46900375-46900394 Msa0814650:intron 30.0%
AAAGCAGCTAAATTCTGTTA+CGG - chr5_3:46900466-46900485 Msa0814650:intron 30.0%
! TTTGACGATGTTTAGTTCAT+CGG + chr5_3:46900683-46900702 None:intergenic 30.0%
!!! TTCGGATTTTGTTTCTGAAT+CGG + chr5_3:46900980-46900999 None:intergenic 30.0%
TAAGATAAATATCGACAGCA+CGG + chr5_3:46901461-46901480 None:intergenic 30.0%
TGCTGTCGATATTTATCTTA+AGG - chr5_3:46901461-46901480 Msa0814650:CDS 30.0%
TTAAGACTGAATTGAGTTCA+AGG + chr5_3:46901503-46901522 None:intergenic 30.0%
TGCACTAAAACTTTCATATG+AGG - chr5_3:46901738-46901757 Msa0814650:intron 30.0%
ATTACAAACAGACGTAACTT+TGG - chr5_3:46901885-46901904 Msa0814650:intron 30.0%
GTTTCAGATTTGCAAAAGAA+CGG - chr5_3:46901958-46901977 Msa0814650:intron 30.0%
CTTACCTAATTTCTTTGACA+CGG + chr5_3:46902049-46902068 None:intergenic 30.0%
!! TCTTTAGAACCATTCTTGAA+CGG + chr5_3:46902078-46902097 None:intergenic 30.0%
TAAAGATACGTCTCATATTG+AGG - chr5_3:46902092-46902111 Msa0814650:intron 30.0%
ATCATCATAGTTTAGTATGG+CGG - chr5_3:46902308-46902327 Msa0814650:five_prime_UTR 30.0%
GTCTTTGTATCAAAGAAGAA+AGG - chr5_3:46902393-46902412 Msa0814650:five_prime_UTR 30.0%
TGTGTACGTATATATACGTT+TGG - chr5_3:46902417-46902436 Msa0814650:five_prime_UTR 30.0%
ACAATGAAGTTAATCCACTG+GGG - chr5_3:46899668-46899687 Msa0814650:three_prime_UTR 35.0%
TGAAAGTGAAAGAGATGAAG+AGG + chr5_3:46899881-46899900 None:intergenic 35.0%
ATGATTAGTGTTAGAGACAC+TGG - chr5_3:46900194-46900213 Msa0814650:CDS 35.0%
AAGCAAGCACAAAACTATGA+AGG + chr5_3:46900278-46900297 None:intergenic 35.0%
ATTGCAAACCTTTGAGCTAA+CGG + chr5_3:46900719-46900738 None:intergenic 35.0%
AATCTGTTGAATCAGATGAC+CGG + chr5_3:46900957-46900976 None:intergenic 35.0%
TTGTAGAATCTCACGTTGTT+CGG + chr5_3:46900998-46901017 None:intergenic 35.0%
AATACCGTTGAACTTCGAAA+TGG + chr5_3:46901391-46901410 None:intergenic 35.0%
ATTGCCATTCTCATTCCAAA+AGG - chr5_3:46901408-46901427 Msa0814650:CDS 35.0%
ATCTCAGAACAAACGATTAC+CGG - chr5_3:46901774-46901793 Msa0814650:intron 35.0%
! AAATGCAATTTGTTTGGGGT+AGG - chr5_3:46902282-46902301 Msa0814650:five_prime_UTR 35.0%
TCATCATAGTTTAGTATGGC+GGG - chr5_3:46902309-46902328 Msa0814650:five_prime_UTR 35.0%
GTACGTATATATACGTTTGG+AGG - chr5_3:46902420-46902439 Msa0814650:five_prime_UTR 35.0%
GACATGTAAGTTTGTCACTG+TGG + chr5_3:46899622-46899641 None:intergenic 40.0%
TACCTTGTGGAGAGAATAAG+TGG + chr5_3:46900022-46900041 None:intergenic 40.0%
CAACATGTAACAGTTCATGC+TGG - chr5_3:46900339-46900358 Msa0814650:CDS 40.0%
CTGCTTTCTCAAAAGCTTCT+TGG + chr5_3:46900453-46900472 None:intergenic 40.0%
AATACTCATCGGTCATCTGA+AGG + chr5_3:46900549-46900568 None:intergenic 40.0%
GAGAAAACCTTCAGTTCTTC+CGG + chr5_3:46900587-46900606 None:intergenic 40.0%
! AACTGAAGGTTTTCTCTCTC+AGG - chr5_3:46900591-46900610 Msa0814650:intron 40.0%
GAAGGATTCAACGTCAAGAA+CGG + chr5_3:46900794-46900813 None:intergenic 40.0%
GTTCTTGACGTTGAATCCTT+CGG - chr5_3:46900793-46900812 Msa0814650:CDS 40.0%
TTCTGGATCTTGACAGCATT+CGG - chr5_3:46901214-46901233 Msa0814650:CDS 40.0%
! TTTCTCCTTTTCGACGAATG+CGG + chr5_3:46901250-46901269 None:intergenic 40.0%
CTCACCATTTCGAAGTTCAA+CGG - chr5_3:46901384-46901403 Msa0814650:CDS 40.0%
CTTCTCTAACTCATCAAGGT+TGG + chr5_3:46901695-46901714 None:intergenic 40.0%
CAACCTTGATGAGTTAGAGA+AGG - chr5_3:46901693-46901712 Msa0814650:CDS 40.0%
TCTCCTTCTCTAACTCATCA+AGG + chr5_3:46901699-46901718 None:intergenic 40.0%
! AGTTGAGAACGGAGTTGATT+AGG - chr5_3:46901923-46901942 Msa0814650:intron 40.0%
GATTTGCAAAAGAACGGTCA+CGG - chr5_3:46901964-46901983 Msa0814650:intron 40.0%
TCGTCCGTGTCAAAGAAATT+AGG - chr5_3:46902042-46902061 Msa0814650:CDS 40.0%
AAGCTGAATCCGTTCAAGAA+TGG - chr5_3:46902066-46902085 Msa0814650:CDS 40.0%
ACGTCTCATATTGAGGATGT+TGG - chr5_3:46902099-46902118 Msa0814650:intron 40.0%
TCATATTGAGGATGTTGGTG+TGG - chr5_3:46902104-46902123 Msa0814650:intron 40.0%
TGGATTTGACAAAGCCTAGA+AGG - chr5_3:46902124-46902143 Msa0814650:intron 40.0%
TAGGTAATGTAGAACCCCAG+TGG + chr5_3:46899685-46899704 None:intergenic 45.0%
AGAGAGAGTCTGCTAGGATT+AGG + chr5_3:46899813-46899832 None:intergenic 45.0%
AGCCACTTATTCTCTCCACA+AGG - chr5_3:46900017-46900036 Msa0814650:CDS 45.0%
CATGTAACAGTTCATGCTGG+TGG - chr5_3:46900342-46900361 Msa0814650:CDS 45.0%
GAGGTCTAATCTGGTGAGTT+CGG + chr5_3:46900413-46900432 None:intergenic 45.0%
ATCGGTCATCTGAAGGAACA+CGG + chr5_3:46900542-46900561 None:intergenic 45.0%
! ATGACCGATGAGTATTGCGA+CGG - chr5_3:46900553-46900572 Msa0814650:intron 45.0%
GTTTCCGTCGCAATACTCAT+CGG + chr5_3:46900560-46900579 None:intergenic 45.0%
! TGAAGGTTTTCTCTCTCAGG+TGG - chr5_3:46900594-46900613 Msa0814650:intron 45.0%
! GTGGCCTTAACAACTCTTTC+CGG - chr5_3:46900613-46900632 Msa0814650:CDS 45.0%
!! ACGGCATGACTTTAGAACGA+CGG + chr5_3:46900644-46900663 None:intergenic 45.0%
! TGTTTAGTTCATCGGCGACT+GGG + chr5_3:46900675-46900694 None:intergenic 45.0%
! ATGTTTAGTTCATCGGCGAC+TGG + chr5_3:46900676-46900695 None:intergenic 45.0%
ATCACCGCAATGAAACAACG+TGG - chr5_3:46900823-46900842 Msa0814650:CDS 45.0%
TGTGTATGCAATCAACGCTC+CGG + chr5_3:46900869-46900888 None:intergenic 45.0%
TTTGATCACGTACAAGCTCC+CGG + chr5_3:46900903-46900922 None:intergenic 45.0%
GAGCTTGTACGTGATCAAAC+CGG - chr5_3:46900904-46900923 Msa0814650:CDS 45.0%
CATCGTCTCTCTCTTTCCAT+CGG - chr5_3:46901020-46901039 Msa0814650:CDS 45.0%
! AGACAGCAGAGGAAGTTGAT+CGG + chr5_3:46901060-46901079 None:intergenic 45.0%
CACGTAGGTAAATAGCGCAA+CGG + chr5_3:46901086-46901105 None:intergenic 45.0%
GTTCTGTCATTCACCTACGA+CGG - chr5_3:46901183-46901202 Msa0814650:CDS 45.0%
!! CGAAAAGGAGAAAAGCACCA+CGG - chr5_3:46901257-46901276 Msa0814650:CDS 45.0%
! TAGGTAAGCGTCCACAGTTT+TGG + chr5_3:46901340-46901359 None:intergenic 45.0%
CTAGCTGAGATTGCTTCCTA+CGG - chr5_3:46901357-46901376 Msa0814650:CDS 45.0%
!!! TGCGCCTTTTGGAATGAGAA+TGG + chr5_3:46901415-46901434 None:intergenic 45.0%
! TCAAGTTTTCGTGCGCCTTT+TGG + chr5_3:46901426-46901445 None:intergenic 45.0%
! CATAACATGTGTGATGGTGC+AGG - chr5_3:46901663-46901682 Msa0814650:CDS 45.0%
AACTTTGGTGAGAGAGAACG+AGG - chr5_3:46901900-46901919 Msa0814650:intron 45.0%
AGAGAACGAGGAGTTGAGAA+CGG - chr5_3:46901912-46901931 Msa0814650:intron 45.0%
! ACAACTTCATCGTCTGGAGT+TGG - chr5_3:46901991-46902010 Msa0814650:intron 45.0%
TGACACGGACGAAAAGAATG+TGG + chr5_3:46902034-46902053 None:intergenic 45.0%
TGACAAAGCCTAGAAGGAGA+AGG - chr5_3:46902130-46902149 Msa0814650:intron 45.0%
ATGGAGAACCTTCTCCTTCT+AGG + chr5_3:46902141-46902160 None:intergenic 45.0%
!! GTATGGCGGGTTTGTTGATT+AGG - chr5_3:46902322-46902341 Msa0814650:five_prime_UTR 45.0%
ATGGCAAGAGAGAGTCTGCT+AGG + chr5_3:46899819-46899838 None:intergenic 50.0%
TAGCAGACTCTCTCTTGCCA+TGG - chr5_3:46899818-46899837 Msa0814650:three_prime_UTR 50.0%
TCTCTTGCCATGGAGAGAAC+AGG - chr5_3:46899828-46899847 Msa0814650:three_prime_UTR 50.0%
CTCTTGCCATGGAGAGAACA+GGG - chr5_3:46899829-46899848 Msa0814650:three_prime_UTR 50.0%
GATGTCGGAGAGGTCTAATC+TGG + chr5_3:46900422-46900441 None:intergenic 50.0%
CAACGACGCAACGTTGTTGA+CGG + chr5_3:46900509-46900528 None:intergenic 50.0%
TATTGCGACGGAAACCTTGC+CGG - chr5_3:46900565-46900584 Msa0814650:intron 50.0%
AACCTTGCCGGAAGAACTGA+AGG - chr5_3:46900577-46900596 Msa0814650:intron 50.0%
AACCTTCAGTTCTTCCGGCA+AGG + chr5_3:46900582-46900601 None:intergenic 50.0%
!! GCATGACTTTAGAACGACGG+CGG + chr5_3:46900641-46900660 None:intergenic 50.0%
!! TGCGGTGATCACTTTTCCGA+AGG + chr5_3:46900812-46900831 None:intergenic 50.0%
GCGAAATACCTAACTCTCGC+CGG - chr5_3:46900847-46900866 Msa0814650:CDS 50.0%
CTTGTACGTGATCAAACCGG+TGG - chr5_3:46900907-46900926 Msa0814650:CDS 50.0%
!! GTGGTGGTGGAGGAAAATTC+CGG - chr5_3:46900935-46900954 Msa0814650:CDS 50.0%
CGATGGAAAGAGAGAGACGA+TGG + chr5_3:46901022-46901041 None:intergenic 50.0%
GGAAAAGCAGCTTTCTCCGA+TGG + chr5_3:46901039-46901058 None:intergenic 50.0%
CATCTCCGCATTCGTCGAAA+AGG - chr5_3:46901242-46901261 Msa0814650:CDS 50.0%
! GTTTTCTCCGAACGTGGTAC+CGG + chr5_3:46901292-46901311 None:intergenic 50.0%
! AGAGCAGTTTTCTCCGAACG+TGG + chr5_3:46901298-46901317 None:intergenic 50.0%
CGTAGGAAGCAATCTCAGCT+AGG + chr5_3:46901359-46901378 None:intergenic 50.0%
CGAAATGGTGAGTTCTCCGT+AGG + chr5_3:46901376-46901395 None:intergenic 50.0%
! GGAGAAAGTGTGCAGTGTGA+TGG - chr5_3:46901714-46901733 Msa0814650:intron 50.0%
TTGCAAAAGAACGGTCACGG+CGG - chr5_3:46901967-46901986 Msa0814650:intron 50.0%
CCATTGCCCTGTTCTCTCCA+TGG + chr5_3:46899838-46899857 None:intergenic 55.0%
CCATGGAGAGAACAGGGCAA+TGG - chr5_3:46899835-46899854 Msa0814650:three_prime_UTR 55.0%
TTAGACCTCTCCGACATCCC+CGG - chr5_3:46900424-46900443 Msa0814650:intron 55.0%
TCAAAAGCTTCTTGGCCGCC+GGG + chr5_3:46900445-46900464 None:intergenic 55.0%
CTCAAAAGCTTCTTGGCCGC+CGG + chr5_3:46900446-46900465 None:intergenic 55.0%
GGTCATCTGAAGGAACACGG+CGG + chr5_3:46900539-46900558 None:intergenic 55.0%
! GGCGCCGGAAAGAGTTGTTA+AGG + chr5_3:46900620-46900639 None:intergenic 55.0%
CGTTGACGCCGTTAGCTCAA+AGG - chr5_3:46900708-46900727 Msa0814650:CDS 55.0%
AATTCGGCGGTGATCGGCTA+GGG + chr5_3:46900753-46900772 None:intergenic 55.0%
TCCACCAATTCGGCGGTGAT+CGG + chr5_3:46900759-46900778 None:intergenic 55.0%
ATCACCGCCGAATTGGTGGA+CGG - chr5_3:46900759-46900778 Msa0814650:CDS 55.0%
AGCTCCTCCGTCCACCAATT+CGG + chr5_3:46900769-46900788 None:intergenic 55.0%
! CGCGCCACGTTGTTTCATTG+CGG + chr5_3:46900830-46900849 None:intergenic 55.0%
CATACACAGAACGCACGCTC+CGG - chr5_3:46900881-46900900 Msa0814650:CDS 55.0%
ATACACAGAACGCACGCTCC+GGG - chr5_3:46900882-46900901 Msa0814650:CDS 55.0%
GTACGTGATCAAACCGGTGG+CGG - chr5_3:46900910-46900929 Msa0814650:CDS 55.0%
TTCACGCTTGCACGAGCTAG+AGG + chr5_3:46901109-46901128 None:intergenic 55.0%
TTCAACGCCGGTACCACGTT+CGG - chr5_3:46901282-46901301 Msa0814650:CDS 55.0%
!! AGTTTTGGCCACGCGTTGCA+TGG + chr5_3:46901325-46901344 None:intergenic 55.0%
GCGTGTAGCACAATCTGCAC+CGG + chr5_3:46901796-46901815 None:intergenic 55.0%
CAGAACGTGACGCAGAAGAG+AGG - chr5_3:46901857-46901876 Msa0814650:intron 55.0%
GGCGGAACAACTTCATCGTC+TGG - chr5_3:46901985-46902004 Msa0814650:intron 55.0%
CAAAAGCTTCTTGGCCGCCG+GGG + chr5_3:46900444-46900463 None:intergenic 60.0%
CAATTCGGCGGTGATCGGCT+AGG + chr5_3:46900754-46900773 None:intergenic 60.0%
CTAGCCGATCACCGCCGAAT+TGG - chr5_3:46900752-46900771 Msa0814650:CDS 60.0%
TCCTCCGTCCACCAATTCGG+CGG + chr5_3:46900766-46900785 None:intergenic 60.0%
TCAACGCTCCGGCGAGAGTT+AGG + chr5_3:46900858-46900877 None:intergenic 60.0%
!! GATCAAACCGGTGGCGGAAG+TGG - chr5_3:46900916-46900935 Msa0814650:CDS 60.0%
CGCAACGGAGAAGACAGCAG+AGG + chr5_3:46901071-46901090 None:intergenic 60.0%
! TGCACGAGCTAGAGGCACGT+AGG + chr5_3:46901101-46901120 None:intergenic 60.0%
CATTCACCTACGACGGCGAG+AGG - chr5_3:46901190-46901209 Msa0814650:CDS 60.0%
AGCACCACGGTGTTCAACGC+CGG - chr5_3:46901270-46901289 Msa0814650:CDS 60.0%
TTTGGCCACGCGTTGCATGG+CGG + chr5_3:46901322-46901341 None:intergenic 60.0%
GCAACGCGTGGCCAAAACTG+TGG - chr5_3:46901326-46901345 Msa0814650:CDS 60.0%
TTCTTGGCCGCCGGGGATGT+CGG + chr5_3:46900437-46900456 None:intergenic 65.0%
!! CTTTAGAACGACGGCGGCGC+CGG + chr5_3:46900635-46900654 None:intergenic 65.0%
CGGCGACTGGGAGAAGCTGA+CGG + chr5_3:46900663-46900682 None:intergenic 65.0%
GCCGATCACCGCCGAATTGG+TGG - chr5_3:46900755-46900774 Msa0814650:CDS 65.0%
! ACCGCCGAATTGGTGGACGG+AGG - chr5_3:46900762-46900781 Msa0814650:CDS 65.0%
!! CAAACCGGTGGCGGAAGTGG+TGG - chr5_3:46900919-46900938 Msa0814650:CDS 65.0%
TCCACCACCACTTCCGCCAC+CGG + chr5_3:46900926-46900945 None:intergenic 65.0%
CAGAAGCCTCTCGCCGTCGT+AGG + chr5_3:46901199-46901218 None:intergenic 65.0%
CTACGACGGCGAGAGGCTTC+TGG - chr5_3:46901197-46901216 Msa0814650:CDS 65.0%
!! GGTACCGGCGTTGAACACCG+TGG + chr5_3:46901277-46901296 None:intergenic 65.0%
GACCTCTCCGACATCCCCGG+CGG - chr5_3:46900427-46900446 Msa0814650:intron 70.0%
!! ACCGGTGGCGGAAGTGGTGG+TGG - chr5_3:46900922-46900941 Msa0814650:CDS 70.0%
!! GGTGGCGGAAGTGGTGGTGG+AGG - chr5_3:46900925-46900944 Msa0814650:CDS 70.0%
CTCTGCCGCCATGCAACGCG+TGG - chr5_3:46901314-46901333 Msa0814650:CDS 70.0%
! GGCCGCCGGGGATGTCGGAG+AGG + chr5_3:46900432-46900451 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 46899533 46902545 46899533 ID=Msa0814650;Name=Msa0814650
chr5_3 mRNA 46899533 46902545 46899533 ID=Msa0814650-mRNA-1;Parent=Msa0814650;Name=Msa0814650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=268|1|1|1|1|1|4|378|579
chr5_3 exon 46899533 46900393 46899533 ID=Msa0814650-mRNA-1:exon:14223;Parent=Msa0814650-mRNA-1
chr5_3 exon 46900596 46901726 46900596 ID=Msa0814650-mRNA-1:exon:14222;Parent=Msa0814650-mRNA-1
chr5_3 exon 46902040 46902109 46902040 ID=Msa0814650-mRNA-1:exon:14221;Parent=Msa0814650-mRNA-1
chr5_3 exon 46902222 46902545 46902222 ID=Msa0814650-mRNA-1:exon:14220;Parent=Msa0814650-mRNA-1
chr5_3 five_prime_UTR 46902278 46902545 46902278 ID=Msa0814650-mRNA-1:five_prime_utr;Parent=Msa0814650-mRNA-1
chr5_3 CDS 46902222 46902277 46902222 ID=Msa0814650-mRNA-1:cds;Parent=Msa0814650-mRNA-1
chr5_3 CDS 46902040 46902109 46902040 ID=Msa0814650-mRNA-1:cds;Parent=Msa0814650-mRNA-1
chr5_3 CDS 46900596 46901726 46900596 ID=Msa0814650-mRNA-1:cds;Parent=Msa0814650-mRNA-1
chr5_3 CDS 46899911 46900393 46899911 ID=Msa0814650-mRNA-1:cds;Parent=Msa0814650-mRNA-1
chr5_3 three_prime_UTR 46899533 46899910 46899533 ID=Msa0814650-mRNA-1:three_prime_utr;Parent=Msa0814650-mRNA-1
Gene Sequence

>Msa0814650

ATGGAGAATCCTAATCCTAGCAGACTCTCTCTTGCCATGGAGAGAACAGGGCAATGGATTTTCTCTCAAGAAATCCCAAGTGATGTTCTAGTTACAATTAGAGAAGCCACTTATTCTCTCCACAAGTTCATGCTGGTGGCAAAGAGCAACTACATCAGAAAACTGATAATGGAATCAAATGAATCCGAACTCACCAGATTAGACCTCTCCGACATCCCCGGCGGCCAAGAAGCTTTTGAGAAAGCAGCTAAATTCTGTTACGGTGTCAACTTCGAGATAACCGTCAACAACGTTGCGTCGTTGCACTGCGCCGCCGTGTTCCTTCAGATGACCGATGAGTATTGCGACGGAAACCTTGCCGGAAGAACTGAAGGTTTTCTCTCTCAGGTGGCCTTAACAACTCTTTCCGGCGCCGCCGTCGTTCTAAAGTCATGCCGTCAGCTTCTCCCAGTCGCCGATGAACTAAACATCGTCAAACGCTGCGTTGACGCCGTTAGCTCAAAGGTTTGCAATGAAGCAAACTTCCCTAGCCGATCACCGCCGAATTGGTGGACGGAGGAGCTCGCCGTTCTTGACGTTGAATCCTTCGGAAAAGTGATCACCGCAATGAAACAACGTGGCGCGAAATACCTAACTCTCGCCGGAGCGTTGATTGCATACACAGAACGCACGCTCCGGGAGCTTGTACGTGATCAAACCGGTGGCGGAAGTGGTGGTGGAGGAAAATTCCGGTCATCTGATTCAACAGATTCCGATTCAGAAACAAAATCCGAACAACGTGAGATTCTACAATCCATCGTCTCTCTCTTTCCATCGGAGAAAGCTGCTTTTCCGATCAACTTCCTCTGCTGTCTTCTCCGTTGCGCTATTTACCTACGTGCCTCTAGCTCGTGCAAGCGTGAACTCGAGAAACGAATTGCTGAAATTCTCGAACACGTAACAGTGAACGATCTCTTAGTTCTGTCATTCACCTACGACGGCGAGAGGCTTCTGGATCTTGACAGCATTCGGAGAATCATCTCCGCATTCGTCGAAAAGGAGAAAAGCACCACGGTGTTCAACGCCGGTACCACGTTCGGAGAAAACTGCTCTGCCGCCATGCAACGCGTGGCCAAAACTGTGGACGCTTACCTAGCTGAGATTGCTTCCTACGGAGAACTCACCATTTCGAAGTTCAACGGTATTGCCATTCTCATTCCAAAAGGCGCACGAAAACTTGACGATGATCTTTACCGTGCTGTCGATATTTATCTTAAGGTACACACCAACCTTGATGAGTTAGAGAAGGAGAAAGTGTGCAGTGTGATGGATGCACTAAAACTTTCATATGAGGCACGTGTTCATGCATCTCAGAACAAACGATTACCGGTGCAGATTGTGCTACACGCGTTATATTACGATCAATTAAAGATAAGAAGTGGGGCAACAGCAGAACGTGACGCAGAAGAGAGGAATCAATTACAAACAGACGTAACTTTGGTGAGAGAGAACGAGGAGTTGAGAACGGAGTTGATTAGGATGAAAATGTATGTTTCAGATTTGCAAAAGAACGGTCACGGCGGAACAACTTCATCGTCTGGAGTTGGAAAAGAAGTCAAAAAGTCCACATTCTTTTCGTCCGTGTCAAAGAAATTAGGTAAGCTGAATCCGTTCAAGAATGGTTCTAAAGATACGTCTCATATTGAGGATGTTGGTGTGGATTTGACAAAGCCTAGAAGGAGAAGGTTCTCCATTTCATAG
Protein sequence

>Msa0814650

MENPNPSRLSLAMERTGQWIFSQEIPSDVLVTIREATYSLHKFMLVAKSNYIRKLIMESNESELTRLDLSDIPGGQEAFEKAAKFCYGVNFEITVNNVASLHCAAVFLQMTDEYCDGNLAGRTEGFLSQVALTTLSGAAVVLKSCRQLLPVADELNIVKRCVDAVSSKVCNEANFPSRSPPNWWTEELAVLDVESFGKVITAMKQRGAKYLTLAGALIAYTERTLRELVRDQTGGGSGGGGKFRSSDSTDSDSETKSEQREILQSIVSLFPSEKAAFPINFLCCLLRCAIYLRASSSCKRELEKRIAEILEHVTVNDLLVLSFTYDGERLLDLDSIRRIISAFVEKEKSTTVFNAGTTFGENCSAAMQRVAKTVDAYLAEIASYGELTISKFNGIAILIPKGARKLDDDLYRAVDIYLKVHTNLDELEKEKVCSVMDALKLSYEARVHASQNKRLPVQIVLHALYYDQLKIRSGATAERDAEERNQLQTDVTLVRENEELRTELIRMKMYVSDLQKNGHGGTTSSSGVGKEVKKSTFFSSVSKKLGKLNPFKNGSKDTSHIEDVGVDLTKPRRRRFSIS*