AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0782120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782120 MtrunA17_Chr2g0310831 30.204 245 126 10 216 456 225 428 2.94e-14 74.7
Msa0782120 MtrunA17_Chr5g0395911 24.629 337 203 16 135 466 119 409 3.15e-12 68.2
Msa0782120 MtrunA17_Chr1g0179091 28.689 244 134 9 216 457 150 355 1.28e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782120 AT5G37540.1 27.536 276 157 15 139 396 137 387 6.48e-14 74.3

Find 83 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGATAATAAGAGAATT+TGG 0.156886 5_2:-64813127 None:intergenic
GAATGACCTTGTTCTGTTAA+AGG 0.180983 5_2:-64813240 None:intergenic
TGATAGTACTAAAGTTAATT+TGG 0.228281 5_2:+64814078 Msa0782120:three_prime_UTR
AAAGTATTATTATTGATATA+AGG 0.241257 5_2:-64814056 None:intergenic
ACTAAATCAAAAGGAATTTA+TGG 0.245370 5_2:+64812925 Msa0782120:CDS
TTTCTAGAACCTGGACATTC+TGG 0.270492 5_2:-64812859 None:intergenic
CCGCAAGAGAAACTCTTCTT+AGG 0.279122 5_2:-64812796 None:intergenic
CATCAAAGGCTGCGTAACTA+TGG 0.292316 5_2:+64813669 Msa0782120:CDS
ATGCCATGCCATTTGGTATA+AGG 0.301822 5_2:+64813858 Msa0782120:CDS
AAGGTAATGATTATTGATTC+AGG 0.310716 5_2:+64813363 Msa0782120:CDS
TTTATGTAGAAATGTTCATT+AGG 0.312982 5_2:+64814180 Msa0782120:three_prime_UTR
TCCTTCAAATCATCACATTC+TGG 0.314277 5_2:-64812823 None:intergenic
TAGGGCACAGATGGATTTCT+TGG 0.326909 5_2:+64813731 Msa0782120:CDS
TTTATGGTCCAGCTAATCTT+TGG 0.343745 5_2:+64812941 Msa0782120:CDS
GGAGTTGTTGGACTTGGATC+TGG 0.346598 5_2:+64813066 Msa0782120:CDS
GAAACACAAAGCAATTATAT+TGG 0.348878 5_2:-64809650 None:intergenic
TATTGGTTCCTTCCACAATC+TGG 0.350819 5_2:-64809633 None:intergenic
CTTGGAAGTGCAACGGGGAC+TGG 0.360881 5_2:+64809603 Msa0782120:CDS
GGAAGATAATAAGAGAATTT+GGG 0.362567 5_2:-64813126 None:intergenic
AAGTATTATTATTGATATAA+GGG 0.372276 5_2:-64814055 None:intergenic
GCTATAAGCAAGTTCACCTT+AGG 0.379444 5_2:+64813183 Msa0782120:CDS
AATCTCCTTGGAAGTGCAAC+GGG 0.380874 5_2:+64809597 Msa0782120:CDS
TGTAATTATACTTTATGATA+AGG 0.383859 5_2:-64814378 None:intergenic
CAAAATTGTTTCGAGAAAGA+TGG 0.384032 5_2:+64813486 Msa0782120:CDS
GTAAGCCATTGAAGAATTAT+CGG 0.389486 5_2:+64813805 Msa0782120:CDS
TTATGTAGAAATGTTCATTA+GGG 0.391161 5_2:+64814181 Msa0782120:three_prime_UTR
AACCAAGGAGTTGTTGGACT+TGG 0.403721 5_2:+64813060 Msa0782120:CDS
GAGAGAGTTGGGAGTGTGCA+TGG 0.419319 5_2:+64814023 Msa0782120:intron
TCATGAGTGTCATCATTTAT+CGG 0.428600 5_2:-64813333 None:intergenic
TCCAGAATGTGATGATTTGA+AGG 0.431291 5_2:+64812822 Msa0782120:CDS
GCCCTACTTCCAAGTATATT+CGG 0.435533 5_2:-64813714 None:intergenic
TGTGATGATTTGAAGGATAT+CGG 0.438665 5_2:+64812829 Msa0782120:CDS
GCAAAGTGTGCTTGAGCAAA+GGG 0.446414 5_2:+64809522 Msa0782120:CDS
TTATTAACTCCAAATTAACT+TGG 0.454252 5_2:-64814127 None:intergenic
GGCACAGATGGATTTCTTGG+TGG 0.464524 5_2:+64813734 Msa0782120:CDS
AAGGACCGATAATTCTTCAA+TGG 0.470737 5_2:-64813810 None:intergenic
TGTATATATGCCATGCCATT+TGG 0.479150 5_2:+64813851 Msa0782120:CDS
GTTGTAAACCAAAGATTAGC+TGG 0.481387 5_2:-64812949 None:intergenic
TGACACTCATGAAGCTCATA+AGG 0.484215 5_2:+64813344 Msa0782120:CDS
GTAGACTGTACTAAATCAAA+AGG 0.484682 5_2:+64812916 Msa0782120:CDS
TCGTCTTTGCGACGAGATTG+TGG 0.487130 5_2:-64813147 None:intergenic
ATCCAAGTCCAACAACTCCT+TGG 0.491502 5_2:-64813062 None:intergenic
AAAATTGTTTCGAGAAAGAT+GGG 0.497335 5_2:+64813487 Msa0782120:CDS
GTTGTTTCTAGAACCGCGTC+AGG 0.497720 5_2:+64809884 Msa0782120:CDS
GAGAAAAGACCGAATATACT+TGG 0.500599 5_2:+64813705 Msa0782120:CDS
ACATTCTAGTGAAATCTCCT+TGG 0.509243 5_2:+64809585 Msa0782120:CDS
GACCGAATATACTTGGAAGT+AGG 0.515777 5_2:+64813712 Msa0782120:CDS
TGCAAAGTGTGCTTGAGCAA+AGG 0.522732 5_2:+64809521 Msa0782120:CDS
TATAGCAGCCTTATACCAAA+TGG 0.522877 5_2:-64813866 None:intergenic
ACCGAATATACTTGGAAGTA+GGG 0.523296 5_2:+64813713 Msa0782120:CDS
ACTTGGAAGTAGGGCACAGA+TGG 0.526284 5_2:+64813722 Msa0782120:CDS
TTTGGAGTTAATAATACCAT+GGG 0.526759 5_2:+64814136 Msa0782120:three_prime_UTR
GAGTTGTTGGACTTGGATCT+GGG 0.528801 5_2:+64813067 Msa0782120:CDS
ATCAAAGGCTGCGTAACTAT+GGG 0.545015 5_2:+64813670 Msa0782120:CDS
CAGTCCCCGTTGCACTTCCA+AGG 0.547760 5_2:-64809602 None:intergenic
GATAAACATAATCTGAACGC+AGG 0.551270 5_2:-64809698 None:intergenic
ATTTGGAGTTAATAATACCA+TGG 0.556947 5_2:+64814135 Msa0782120:three_prime_UTR
TATATATGCTTAACAGTGCA+AGG 0.561224 5_2:+64813630 Msa0782120:CDS
AAATTCCACACCGTGCCCTA+AGG 0.561293 5_2:-64813199 None:intergenic
ACACATATTCTTATTCTGAA+AGG 0.569596 5_2:-64813829 None:intergenic
TTGATTGTGTTCTTGAGCAT+AGG 0.571289 5_2:+64809490 Msa0782120:CDS
GGCATCAAACCAGAATGTCC+AGG 0.585165 5_2:+64812850 Msa0782120:CDS
AAATCTCCTTGGAAGTGCAA+CGG 0.586573 5_2:+64809596 Msa0782120:CDS
CATCTTAAAAGTATTTCTGT+AGG 0.587267 5_2:+64813288 Msa0782120:CDS
ACTGGACTACTTCCAGATTG+TGG 0.590531 5_2:+64809621 Msa0782120:CDS
CTATAAGCAAGTTCACCTTA+GGG 0.591792 5_2:+64813184 Msa0782120:CDS
CAGCCTTATACCAAATGGCA+TGG 0.599563 5_2:-64813861 None:intergenic
AGCACTCCTTTAACAGAACA+AGG 0.603395 5_2:+64813234 Msa0782120:CDS
TTCTTGACACACATGACTAG+AGG 0.604107 5_2:-64813892 None:intergenic
GACTACTTCCAGATTGTGGA+AGG 0.605667 5_2:+64809625 Msa0782120:CDS
AAAATGAAAAGTATCATCAA+AGG 0.605993 5_2:+64813655 Msa0782120:CDS
AGCAAGTTCACCTTAGGGCA+CGG 0.611602 5_2:+64813189 Msa0782120:CDS
ATAAACATAATCTGAACGCA+GGG 0.615500 5_2:-64809697 None:intergenic
TCAGTTGTTAGAATGACCCA+TGG 0.639074 5_2:-64814152 None:intergenic
TTATAAAGAACCCAACGAGG+AGG 0.660088 5_2:+64813599 Msa0782120:CDS
CCTAAGAAGAGTTTCTCTTG+CGG 0.660827 5_2:+64812796 Msa0782120:CDS
GCGTAACTATGGGAAGTCAG+AGG 0.678639 5_2:+64813680 Msa0782120:CDS
TAACTATGGGAAGTCAGAGG+AGG 0.678721 5_2:+64813683 Msa0782120:CDS
TGTCAAGAATAATAAATGTG+TGG 0.688701 5_2:+64813906 Msa0782120:three_prime_UTR
GTTCACCTTAGGGCACGGTG+TGG 0.689834 5_2:+64813194 Msa0782120:CDS
TTCTTATAAAGAACCCAACG+AGG 0.696416 5_2:+64813596 Msa0782120:CDS
ATCTCCTTGGAAGTGCAACG+GGG 0.700856 5_2:+64809598 Msa0782120:CDS
TAAACATAATCTGAACGCAG+GGG 0.725156 5_2:-64809696 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATAAAATAAATAA+AGG - chr5_2:64812399-64812418 None:intergenic 0.0%
!!! ATTTTTTTTTTTTATATTAA+AGG + chr5_2:64812703-64812722 Msa0782120:intron 0.0%
!!! CTTATTTTTTTTTTAACATT+TGG + chr5_2:64810479-64810498 Msa0782120:intron 10.0%
!! AATAAAAAAAAATGAGAAAA+AGG + chr5_2:64811265-64811284 Msa0782120:intron 10.0%
!! AATTCTTAAATAAAAACATT+TGG + chr5_2:64811927-64811946 Msa0782120:intron 10.0%
!!! TTTTTTTTTTATATTAAAGG+TGG + chr5_2:64812706-64812725 Msa0782120:intron 10.0%
!! AAGTATTATTATTGATATAA+GGG - chr5_2:64814058-64814077 None:intergenic 10.0%
!!! AAAGTATTATTATTGATATA+AGG - chr5_2:64814059-64814078 None:intergenic 10.0%
!! TAACATTAATTCATGATATT+TGG + chr5_2:64811090-64811109 Msa0782120:intron 15.0%
!! AATCAAAATGTAATATACAT+TGG - chr5_2:64811532-64811551 None:intergenic 15.0%
!!! AATGTATATTACATTTTGAT+TGG + chr5_2:64811531-64811550 Msa0782120:intron 15.0%
!!! CTTTTATAGAAAAATACTAA+CGG + chr5_2:64812016-64812035 Msa0782120:intron 15.0%
!!! ACACTTGTTTTTTTTTTTTT+TGG - chr5_2:64812243-64812262 None:intergenic 15.0%
!! AAAAAGTAGAAAAAGAATAT+GGG + chr5_2:64813433-64813452 Msa0782120:CDS 15.0%
!!! AAAGACAATTTAAAGTTTTA+AGG + chr5_2:64814277-64814296 Msa0782120:three_prime_UTR 15.0%
!!! AAGACAATTTAAAGTTTTAA+GGG + chr5_2:64814278-64814297 Msa0782120:three_prime_UTR 15.0%
!!! CTTTTTGATTTGATTTTTCT+TGG - chr5_2:64810155-64810174 None:intergenic 20.0%
!! ACAAAATTGAACAATCAAAA+TGG + chr5_2:64810294-64810313 Msa0782120:intron 20.0%
!! CAAAATTGAACAATCAAAAT+GGG + chr5_2:64810295-64810314 Msa0782120:intron 20.0%
!! TGAACACCTAAATATAATTA+AGG + chr5_2:64810431-64810450 Msa0782120:intron 20.0%
!! ATCTTGCCTTAATTATATTT+AGG - chr5_2:64810440-64810459 None:intergenic 20.0%
!! TAACATTAATTCACGATATT+TGG - chr5_2:64810743-64810762 None:intergenic 20.0%
!!! TCTTTATTTTTTTTCCGTTT+CGG - chr5_2:64811323-64811342 None:intergenic 20.0%
!! CGGAAAAAAAATAAAGATAA+AGG + chr5_2:64811326-64811345 Msa0782120:intron 20.0%
!! CTTATTTATTTGTAACACTT+TGG - chr5_2:64811353-64811372 None:intergenic 20.0%
!! TATAGACAAGATTACATTAT+AGG - chr5_2:64811384-64811403 None:intergenic 20.0%
!!! TATTTTCAAAAATATCTACG+TGG + chr5_2:64811637-64811656 Msa0782120:intron 20.0%
!! TGCAAGTAATATAAATTAGA+TGG + chr5_2:64811746-64811765 Msa0782120:intron 20.0%
!! AATATAAATTAGATGGTACA+AGG + chr5_2:64811753-64811772 Msa0782120:intron 20.0%
!! GATTGAAATGTTCATTTATT+TGG + chr5_2:64811986-64812005 Msa0782120:intron 20.0%
!!! AAAAAAAAACAAGTGTCATT+AGG + chr5_2:64812247-64812266 Msa0782120:intron 20.0%
!!! AAAAAAAACAAGTGTCATTA+GGG + chr5_2:64812248-64812267 Msa0782120:intron 20.0%
!! CCAAAATTGTCTTATAAAAA+GGG - chr5_2:64812341-64812360 None:intergenic 20.0%
!! ACCAAAATTGTCTTATAAAA+AGG - chr5_2:64812342-64812361 None:intergenic 20.0%
!!! TAAAATAAATAAAGGTATGC+TGG - chr5_2:64812391-64812410 None:intergenic 20.0%
!! ACTAAATCAAAAGGAATTTA+TGG + chr5_2:64812925-64812944 Msa0782120:CDS 20.0%
!!! AATGTTCAATCTTTCTTTTT+TGG - chr5_2:64812989-64813008 None:intergenic 20.0%
!! AAAAAGAAAGATTGAACATT+TGG + chr5_2:64812989-64813008 Msa0782120:CDS 20.0%
!! GAAAAAGTAGAAAAAGAATA+TGG + chr5_2:64813432-64813451 Msa0782120:CDS 20.0%
!!! ATATAAAAATTGAGTTGAAG+AGG + chr5_2:64813553-64813572 Msa0782120:CDS 20.0%
!!! TATAAAAATTGAGTTGAAGA+GGG + chr5_2:64813554-64813573 Msa0782120:CDS 20.0%
!!! AAAATGAAAAGTATCATCAA+AGG + chr5_2:64813655-64813674 Msa0782120:CDS 20.0%
!!! TATTAATAAAGTGTCTTTTG+AGG + chr5_2:64813776-64813795 Msa0782120:CDS 20.0%
!! TGATAGTACTAAAGTTAATT+TGG + chr5_2:64814078-64814097 Msa0782120:three_prime_UTR 20.0%
!!! TATTTTACTCCAAGTTAATT+TGG + chr5_2:64814118-64814137 Msa0782120:three_prime_UTR 20.0%
!! TTATTAACTCCAAATTAACT+TGG - chr5_2:64814130-64814149 None:intergenic 20.0%
!! TTTATGTAGAAATGTTCATT+AGG + chr5_2:64814180-64814199 Msa0782120:three_prime_UTR 20.0%
!! TTATGTAGAAATGTTCATTA+GGG + chr5_2:64814181-64814200 Msa0782120:three_prime_UTR 20.0%
!!! AGACAATTTAAAGTTTTAAG+GGG + chr5_2:64814279-64814298 Msa0782120:three_prime_UTR 20.0%
! GAAACACAAAGCAATTATAT+TGG - chr5_2:64809653-64809672 None:intergenic 25.0%
! TAGCAATCCTTAAAGTATAA+CGG + chr5_2:64810092-64810111 Msa0782120:intron 25.0%
! GAATGTAACCGATAAATTTA+AGG + chr5_2:64810333-64810352 Msa0782120:intron 25.0%
! CTGAATGTCCTTAAATTTAT+CGG - chr5_2:64810344-64810363 None:intergenic 25.0%
! ATTAATTTACACTACAAGAC+TGG - chr5_2:64810405-64810424 None:intergenic 25.0%
! AATTAAGGCAAGATTACATT+AGG + chr5_2:64810446-64810465 Msa0782120:intron 25.0%
! ATTAAGGCAAGATTACATTA+GGG + chr5_2:64810447-64810466 Msa0782120:intron 25.0%
!!! ATCTTTATTTTTTCCCGTTT+AGG + chr5_2:64810508-64810527 Msa0782120:intron 25.0%
!!! TGTGATTTTAAGAGAGATAA+AGG - chr5_2:64810535-64810554 None:intergenic 25.0%
! AACATCTTCATCATATTTCT+AGG - chr5_2:64811187-64811206 None:intergenic 25.0%
! TGTGTTTAAAAGAAGATTTG+AGG - chr5_2:64811061-64811080 None:intergenic 25.0%
! TTGCAACATTTGTGAAATTA+AGG - chr5_2:64810805-64810824 None:intergenic 25.0%
! TTAATTTCACAAATGTTGCA+AGG + chr5_2:64810804-64810823 Msa0782120:intron 25.0%
! TACAAAAGTTAAGATTGTTC+TGG + chr5_2:64810868-64810887 Msa0782120:intron 25.0%
!! ATACTAAATCTCTTTTCTAC+AGG + chr5_2:64811027-64811046 Msa0782120:intron 25.0%
!! TACTAAATCTCTTTTCTACA+GGG + chr5_2:64811028-64811047 Msa0782120:intron 25.0%
! TGTGTTTAAAAGAAGATTTG+AGG + chr5_2:64811061-64811080 Msa0782120:intron 25.0%
!!! CTTTATTTTTTTTCCGTTTC+GGG - chr5_2:64811322-64811341 None:intergenic 25.0%
!!! TAGAAAAAACCCACTTTTAT+AGG + chr5_2:64811601-64811620 Msa0782120:intron 25.0%
! AGAAACTAACCTATAAAAGT+GGG - chr5_2:64811613-64811632 None:intergenic 25.0%
! TAGAAACTAACCTATAAAAG+TGG - chr5_2:64811614-64811633 None:intergenic 25.0%
! GTTGTAACCAAAAAAAAAGA+TGG + chr5_2:64811783-64811802 Msa0782120:intron 25.0%
! CAAATTGATGTTATAAGTCA+AGG - chr5_2:64811835-64811854 None:intergenic 25.0%
! CTTGACTTATAACATCAATT+TGG + chr5_2:64811833-64811852 Msa0782120:intron 25.0%
! AATGCAAACCAATGTTAAAT+GGG - chr5_2:64812161-64812180 None:intergenic 25.0%
! AAATGCAAACCAATGTTAAA+TGG - chr5_2:64812162-64812181 None:intergenic 25.0%
!! CCCTTTTTATAAGACAATTT+TGG + chr5_2:64812338-64812357 Msa0782120:intron 25.0%
! ATAAACTAAGTCTCTCTTAA+AGG + chr5_2:64812453-64812472 Msa0782120:intron 25.0%
! ACCAAAAGAACCATATATTT+AGG + chr5_2:64812550-64812569 Msa0782120:intron 25.0%
!! TCCTAAATATATGGTTCTTT+TGG - chr5_2:64812554-64812573 None:intergenic 25.0%
! AAGAACCATATATTTAGGAA+AGG + chr5_2:64812555-64812574 Msa0782120:intron 25.0%
! TACATCCTTTCCTAAATATA+TGG - chr5_2:64812563-64812582 None:intergenic 25.0%
!! CAAGATACAATTTTCTCTTA+TGG + chr5_2:64812644-64812663 Msa0782120:intron 25.0%
! GGAAGATAATAAGAGAATTT+GGG - chr5_2:64813129-64813148 None:intergenic 25.0%
! TGGAAGATAATAAGAGAATT+TGG - chr5_2:64813130-64813149 None:intergenic 25.0%
!!! CTACAGAAATACTTTTAAGA+TGG - chr5_2:64813290-64813309 None:intergenic 25.0%
!!! CATCTTAAAAGTATTTCTGT+AGG + chr5_2:64813288-64813307 Msa0782120:CDS 25.0%
!!! AAGGTAATGATTATTGATTC+AGG + chr5_2:64813363-64813382 Msa0782120:CDS 25.0%
! AGAAAAAGAATATGGGAAAA+AGG + chr5_2:64813440-64813459 Msa0782120:CDS 25.0%
! GAAAAAGAATATGGGAAAAA+GGG + chr5_2:64813441-64813460 Msa0782120:CDS 25.0%
! AAAATTGTTTCGAGAAAGAT+GGG + chr5_2:64813487-64813506 Msa0782120:CDS 25.0%
! ACACATATTCTTATTCTGAA+AGG - chr5_2:64813832-64813851 None:intergenic 25.0%
! TGTCAAGAATAATAAATGTG+TGG + chr5_2:64813906-64813925 Msa0782120:three_prime_UTR 25.0%
!! GTATGTAGTTTTCAATAAGT+TGG + chr5_2:64813935-64813954 Msa0782120:three_prime_UTR 25.0%
!! ATTTGGAGTTAATAATACCA+TGG + chr5_2:64814135-64814154 Msa0782120:three_prime_UTR 25.0%
!! TTTGGAGTTAATAATACCAT+GGG + chr5_2:64814136-64814155 Msa0782120:three_prime_UTR 25.0%
! TTCAAACAAGAAGTCAAAAA+TGG + chr5_2:64814228-64814247 Msa0782120:three_prime_UTR 25.0%
ATAAACATAATCTGAACGCA+GGG - chr5_2:64809700-64809719 None:intergenic 30.0%
! GTTAGCAGATTTTATTAGGT+TGG - chr5_2:64809936-64809955 None:intergenic 30.0%
!! GTTCTTTTGTTTGGACTTAA+AGG - chr5_2:64809990-64810009 None:intergenic 30.0%
! GAGGTTTTTGTTCTTTTGTT+TGG - chr5_2:64809999-64810018 None:intergenic 30.0%
AAGTCATCCGTTATACTTTA+AGG - chr5_2:64810102-64810121 None:intergenic 30.0%
!! TTTGATTTTTCTTGGTTGTG+AGG - chr5_2:64810147-64810166 None:intergenic 30.0%
TTAAGGCAAGATTACATTAG+GGG + chr5_2:64810448-64810467 Msa0782120:intron 30.0%
AGAAGATGATGAAGATTCAA+AGG - chr5_2:64810688-64810707 None:intergenic 30.0%
!!! TTTTGGGGTTATTTTTATGC+TGG + chr5_2:64810925-64810944 Msa0782120:intron 30.0%
!!! TTTGGGGTTATTTTTATGCT+GGG + chr5_2:64810926-64810945 Msa0782120:intron 30.0%
TTCATCATCTTCATTGAATC+AGG - chr5_2:64811139-64811158 None:intergenic 30.0%
GAAGATTCAAAGAAAGAAGA+TGG + chr5_2:64811156-64811175 Msa0782120:intron 30.0%
GAACATCTTCATCATATTTC+TGG + chr5_2:64811186-64811205 Msa0782120:intron 30.0%
! TTTGATTGGTTGATATCATG+AGG + chr5_2:64811545-64811564 Msa0782120:intron 30.0%
! TTGATTGGTTGATATCATGA+GGG + chr5_2:64811546-64811565 Msa0782120:intron 30.0%
TCTACGTGGAATGATTTAAT+CGG + chr5_2:64811651-64811670 Msa0782120:intron 30.0%
!!! CCTTGTACCATCTTTTTTTT+TGG - chr5_2:64811793-64811812 None:intergenic 30.0%
CCAAAAAAAAAGATGGTACA+AGG + chr5_2:64811790-64811809 Msa0782120:intron 30.0%
! ATAAGTCAAGGTCTTATAAC+TGG - chr5_2:64811823-64811842 None:intergenic 30.0%
AAAATACTAACGGTTGAAGT+TGG + chr5_2:64812026-64812045 Msa0782120:intron 30.0%
! CATACATTGCTCAAATTTTC+AGG + chr5_2:64812068-64812087 Msa0782120:intron 30.0%
AAAACTAACTCAAAGGATGA+CGG - chr5_2:64812122-64812141 None:intergenic 30.0%
!!! TCATCCTTTGAGTTAGTTTT+TGG + chr5_2:64812122-64812141 Msa0782120:intron 30.0%
CATACCAAAAACTAACTCAA+AGG - chr5_2:64812129-64812148 None:intergenic 30.0%
! GACACTTGTTAGTGTTAAAT+GGG - chr5_2:64812207-64812226 None:intergenic 30.0%
! TGACACTTGTTAGTGTTAAA+TGG - chr5_2:64812208-64812227 None:intergenic 30.0%
! TAACACTAACAAGTGTCATT+AGG + chr5_2:64812210-64812229 Msa0782120:intron 30.0%
! AACACTAACAAGTGTCATTA+GGG + chr5_2:64812211-64812230 Msa0782120:intron 30.0%
TGTGATGATTTGAAGGATAT+CGG + chr5_2:64812829-64812848 Msa0782120:CDS 30.0%
!!! TCTTTATCTTTTCTAGAACC+TGG - chr5_2:64812871-64812890 None:intergenic 30.0%
GTAGACTGTACTAAATCAAA+AGG + chr5_2:64812916-64812935 Msa0782120:CDS 30.0%
!!! ATCATCTTTCTTTTTTGTGC+TGG - chr5_2:64813028-64813047 None:intergenic 30.0%
TCATGAGTGTCATCATTTAT+CGG - chr5_2:64813336-64813355 None:intergenic 30.0%
CAAAATTGTTTCGAGAAAGA+TGG + chr5_2:64813486-64813505 Msa0782120:CDS 30.0%
TATATATGCTTAACAGTGCA+AGG + chr5_2:64813630-64813649 Msa0782120:CDS 30.0%
GTGGCTTTCAATCTTAAAAA+AGG + chr5_2:64813753-64813772 Msa0782120:CDS 30.0%
GTAAGCCATTGAAGAATTAT+CGG + chr5_2:64813805-64813824 Msa0782120:CDS 30.0%
TTGATTGTGTTCTTGAGCAT+AGG + chr5_2:64809490-64809509 Msa0782120:CDS 35.0%
ACATTCTAGTGAAATCTCCT+TGG + chr5_2:64809585-64809604 Msa0782120:CDS 35.0%
TAAACATAATCTGAACGCAG+GGG - chr5_2:64809699-64809718 None:intergenic 35.0%
GATAAACATAATCTGAACGC+AGG - chr5_2:64809701-64809720 None:intergenic 35.0%
AGGTGAGAAAAACACAAGAA+GGG - chr5_2:64809810-64809829 None:intergenic 35.0%
AAGGTGAGAAAAACACAAGA+AGG - chr5_2:64809811-64809830 None:intergenic 35.0%
TTGGAACGACAAAATTGTGA+AGG - chr5_2:64809830-64809849 None:intergenic 35.0%
! AATTTGAAACTGCAGCAGTT+TGG - chr5_2:64809849-64809868 None:intergenic 35.0%
! GCGTGTTAGCAGATTTTATT+AGG - chr5_2:64809940-64809959 None:intergenic 35.0%
TTGTTTGGACTTAAAGGCTT+TGG - chr5_2:64809984-64810003 None:intergenic 35.0%
!!! TTTTTTGGTTGCTGCTGAAA+TGG - chr5_2:64810031-64810050 None:intergenic 35.0%
!!! GGAGTTTGCTAGCATTTTTT+TGG - chr5_2:64810046-64810065 None:intergenic 35.0%
AAACTCCCAAAACATCACAA+TGG + chr5_2:64810059-64810078 Msa0782120:intron 35.0%
! TGTTGCCATTGTGATGTTTT+GGG - chr5_2:64810067-64810086 None:intergenic 35.0%
! ATACTTTAAGGATTGCTAGC+AGG - chr5_2:64810090-64810109 None:intergenic 35.0%
AGAGAGATAAAGGACCTAAA+CGG - chr5_2:64810525-64810544 None:intergenic 35.0%
ATTTCACAAATGTTGCAAGG+CGG + chr5_2:64810807-64810826 Msa0782120:intron 35.0%
! TGATTGGTTGATATCATGAG+GGG + chr5_2:64811547-64811566 Msa0782120:intron 35.0%
AAATACCCTCTAAACAAGAG+AGG + chr5_2:64811572-64811591 Msa0782120:intron 35.0%
AATACCCTCTAAACAAGAGA+GGG + chr5_2:64811573-64811592 Msa0782120:intron 35.0%
! ATTACCCTCTCTTGTTTAGA+GGG - chr5_2:64811580-64811599 None:intergenic 35.0%
AATTAGATGGTACAAGGTGT+TGG + chr5_2:64811759-64811778 Msa0782120:intron 35.0%
ACCAACTTTCGTAATTCATG+TGG + chr5_2:64812523-64812542 Msa0782120:intron 35.0%
ACCACATGAATTACGAAAGT+TGG - chr5_2:64812527-64812546 None:intergenic 35.0%
TGTGTTAATTGTAAGTGCAC+AGG - chr5_2:64812605-64812624 None:intergenic 35.0%
TCCAGAATGTGATGATTTGA+AGG + chr5_2:64812822-64812841 Msa0782120:CDS 35.0%
TCCTTCAAATCATCACATTC+TGG - chr5_2:64812826-64812845 None:intergenic 35.0%
TTTATGGTCCAGCTAATCTT+TGG + chr5_2:64812941-64812960 Msa0782120:CDS 35.0%
GTTGTAAACCAAAGATTAGC+TGG - chr5_2:64812952-64812971 None:intergenic 35.0%
!!! TCTTTCTTTTTTGTGCTGGT+TGG - chr5_2:64813024-64813043 None:intergenic 35.0%
GATGAAGAACCAAAAAACCA+AGG + chr5_2:64813045-64813064 Msa0782120:CDS 35.0%
CTATAAGCAAGTTCACCTTA+GGG + chr5_2:64813184-64813203 Msa0782120:CDS 35.0%
GAATGACCTTGTTCTGTTAA+AGG - chr5_2:64813243-64813262 None:intergenic 35.0%
TTCTTATAAAGAACCCAACG+AGG + chr5_2:64813596-64813615 Msa0782120:CDS 35.0%
! TAATGTTTTTCCTCCTCGTT+GGG - chr5_2:64813612-64813631 None:intergenic 35.0%
! ATAATGTTTTTCCTCCTCGT+TGG - chr5_2:64813613-64813632 None:intergenic 35.0%
GAGAAAAGACCGAATATACT+TGG + chr5_2:64813705-64813724 Msa0782120:CDS 35.0%
ACCGAATATACTTGGAAGTA+GGG + chr5_2:64813713-64813732 Msa0782120:CDS 35.0%
AAGGACCGATAATTCTTCAA+TGG - chr5_2:64813813-64813832 None:intergenic 35.0%
TGTATATATGCCATGCCATT+TGG + chr5_2:64813851-64813870 Msa0782120:CDS 35.0%
! TATAGCAGCCTTATACCAAA+TGG - chr5_2:64813869-64813888 None:intergenic 35.0%
! AAATCTCCTTGGAAGTGCAA+CGG + chr5_2:64809596-64809615 Msa0782120:CDS 40.0%
!! TATTGGTTCCTTCCACAATC+TGG - chr5_2:64809636-64809655 None:intergenic 40.0%
! GACCAGTGTGTTCAACTTTT+AGG + chr5_2:64809749-64809768 Msa0782120:intron 40.0%
CTCCTAAAAGTTGAACACAC+TGG - chr5_2:64809754-64809773 None:intergenic 40.0%
TGAGAAAAACACAAGAAGGG+GGG - chr5_2:64809807-64809826 None:intergenic 40.0%
GTGAGAAAAACACAAGAAGG+GGG - chr5_2:64809808-64809827 None:intergenic 40.0%
GGTGAGAAAAACACAAGAAG+GGG - chr5_2:64809809-64809828 None:intergenic 40.0%
AAAAATCACGATACCTGACG+CGG - chr5_2:64809900-64809919 None:intergenic 40.0%
GCTGAAATGGTTATGTCATG+AGG - chr5_2:64810018-64810037 None:intergenic 40.0%
! GTGTTGCCATTGTGATGTTT+TGG - chr5_2:64810068-64810087 None:intergenic 40.0%
!! TGACGTGGTTCTGGAAATAA+CGG + chr5_2:64810195-64810214 Msa0782120:intron 40.0%
ACGGTGATTCTCACGATATT+TGG + chr5_2:64810214-64810233 Msa0782120:intron 40.0%
GAGAGATAAAGGACCTAAAC+GGG - chr5_2:64810524-64810543 None:intergenic 40.0%
! TGCAAAGCTGCTCTCAATTT+TGG + chr5_2:64810908-64810927 Msa0782120:intron 40.0%
! GCAAAGCTGCTCTCAATTTT+GGG + chr5_2:64810909-64810928 Msa0782120:intron 40.0%
! CAAAGCTGCTCTCAATTTTG+GGG + chr5_2:64810910-64810929 Msa0782120:intron 40.0%
GAGAGATAAAAGACCCGAAA+CGG + chr5_2:64811306-64811325 Msa0782120:intron 40.0%
! GATTGGTTGATATCATGAGG+GGG + chr5_2:64811548-64811567 Msa0782120:intron 40.0%
! GATTACCCTCTCTTGTTTAG+AGG - chr5_2:64811581-64811600 None:intergenic 40.0%
GATCGAGACCCATTTAACAT+TGG + chr5_2:64812150-64812169 Msa0782120:intron 40.0%
CCTAAGAAGAGTTTCTCTTG+CGG + chr5_2:64812796-64812815 Msa0782120:CDS 40.0%
!! TTTCTAGAACCTGGACATTC+TGG - chr5_2:64812862-64812881 None:intergenic 40.0%
!!! CCAACAACTCCTTGGTTTTT+TGG - chr5_2:64813057-64813076 None:intergenic 40.0%
CCAAAAAACCAAGGAGTTGT+TGG + chr5_2:64813054-64813073 Msa0782120:CDS 40.0%
GCTATAAGCAAGTTCACCTT+AGG + chr5_2:64813183-64813202 Msa0782120:CDS 40.0%
AGCACTCCTTTAACAGAACA+AGG + chr5_2:64813234-64813253 Msa0782120:CDS 40.0%
TGACACTCATGAAGCTCATA+AGG + chr5_2:64813344-64813363 Msa0782120:CDS 40.0%
GGGAAAAAGGGAGAAAAGAA+AGG + chr5_2:64813453-64813472 Msa0782120:CDS 40.0%
TTATAAAGAACCCAACGAGG+AGG + chr5_2:64813599-64813618 Msa0782120:CDS 40.0%
ATCAAAGGCTGCGTAACTAT+GGG + chr5_2:64813670-64813689 Msa0782120:CDS 40.0%
GACCGAATATACTTGGAAGT+AGG + chr5_2:64813712-64813731 Msa0782120:CDS 40.0%
GCCCTACTTCCAAGTATATT+CGG - chr5_2:64813717-64813736 None:intergenic 40.0%
! ATGCCATGCCATTTGGTATA+AGG + chr5_2:64813858-64813877 Msa0782120:CDS 40.0%
TTCTTGACACACATGACTAG+AGG - chr5_2:64813895-64813914 None:intergenic 40.0%
TCAGTTGTTAGAATGACCCA+TGG - chr5_2:64814155-64814174 None:intergenic 40.0%
TGCAAAGTGTGCTTGAGCAA+AGG + chr5_2:64809521-64809540 Msa0782120:CDS 45.0%
GCAAAGTGTGCTTGAGCAAA+GGG + chr5_2:64809522-64809541 Msa0782120:CDS 45.0%
! AATCTCCTTGGAAGTGCAAC+GGG + chr5_2:64809597-64809616 Msa0782120:CDS 45.0%
ACTGGACTACTTCCAGATTG+TGG + chr5_2:64809621-64809640 Msa0782120:CDS 45.0%
GACTACTTCCAGATTGTGGA+AGG + chr5_2:64809625-64809644 Msa0782120:CDS 45.0%
! AAAGGCTTTGGCTGATAGTG+AGG - chr5_2:64809972-64809991 None:intergenic 45.0%
! GTTATTTCCAGAACCACGTC+AGG - chr5_2:64810196-64810215 None:intergenic 45.0%
CCGCAAGAGAAACTCTTCTT+AGG - chr5_2:64812799-64812818 None:intergenic 45.0%
AACCAAGGAGTTGTTGGACT+TGG + chr5_2:64813060-64813079 Msa0782120:CDS 45.0%
ATCCAAGTCCAACAACTCCT+TGG - chr5_2:64813065-64813084 None:intergenic 45.0%
GAGTTGTTGGACTTGGATCT+GGG + chr5_2:64813067-64813086 Msa0782120:CDS 45.0%
CATCAAAGGCTGCGTAACTA+TGG + chr5_2:64813669-64813688 Msa0782120:CDS 45.0%
TAACTATGGGAAGTCAGAGG+AGG + chr5_2:64813683-64813702 Msa0782120:CDS 45.0%
! TAGGGCACAGATGGATTTCT+TGG + chr5_2:64813731-64813750 Msa0782120:CDS 45.0%
! CAGCCTTATACCAAATGGCA+TGG - chr5_2:64813864-64813883 None:intergenic 45.0%
TGTGAGAGAGAGAGAGAGTT+GGG + chr5_2:64814012-64814031 Msa0782120:intron 45.0%
!! TTAAAAAAAAAATAAGATAA+AGG - chr5_2:64810475-64810494 None:intergenic 5.0%
!! TAGAAAATAATAATAATATA+CGG + chr5_2:64810963-64810982 Msa0782120:intron 5.0%
!!! ATAAAAAAAAAATCTATTTT+AGG - chr5_2:64811472-64811491 None:intergenic 5.0%
!! ATTGAAAATTATTATAAATA+AGG - chr5_2:64811910-64811929 None:intergenic 5.0%
!!! TTTAAAATAAAATTTCTTTT+TGG + chr5_2:64814316-64814335 Msa0782120:three_prime_UTR 5.0%
! ATCTCCTTGGAAGTGCAACG+GGG + chr5_2:64809598-64809617 Msa0782120:CDS 50.0%
!! GTTGTTTCTAGAACCGCGTC+AGG + chr5_2:64809884-64809903 Msa0782120:CDS 50.0%
TCAACTCACCATACCTGACG+TGG + chr5_2:64810180-64810199 Msa0782120:intron 50.0%
TTCCAGAACCACGTCAGGTA+TGG - chr5_2:64810191-64810210 None:intergenic 50.0%
CACAAATGTTGCAAGGCGGA+TGG + chr5_2:64810811-64810830 Msa0782120:intron 50.0%
AAATGTTGCAAGGCGGATGG+TGG + chr5_2:64810814-64810833 Msa0782120:intron 50.0%
AGGCTTAGTCACTAGCGGAA+GGG + chr5_2:64810837-64810856 Msa0782120:intron 50.0%
TAGTCACTAGCGGAAGGGTT+AGG + chr5_2:64810842-64810861 Msa0782120:intron 50.0%
GGCATCAAACCAGAATGTCC+AGG + chr5_2:64812850-64812869 Msa0782120:CDS 50.0%
GGAGTTGTTGGACTTGGATC+TGG + chr5_2:64813066-64813085 Msa0782120:CDS 50.0%
TCGTCTTTGCGACGAGATTG+TGG - chr5_2:64813150-64813169 None:intergenic 50.0%
AGCAAGTTCACCTTAGGGCA+CGG + chr5_2:64813189-64813208 Msa0782120:CDS 50.0%
AAATTCCACACCGTGCCCTA+AGG - chr5_2:64813202-64813221 None:intergenic 50.0%
! AGGGCACGGTGTGGAATTTT+TGG + chr5_2:64813203-64813222 Msa0782120:CDS 50.0%
GCGTAACTATGGGAAGTCAG+AGG + chr5_2:64813680-64813699 Msa0782120:CDS 50.0%
ACTTGGAAGTAGGGCACAGA+TGG + chr5_2:64813722-64813741 Msa0782120:CDS 50.0%
! GGCACAGATGGATTTCTTGG+TGG + chr5_2:64813734-64813753 Msa0782120:CDS 50.0%
GTGTGAGAGAGAGAGAGAGT+TGG + chr5_2:64814011-64814030 Msa0782120:intron 50.0%
! GCTTTGGCTGATAGTGAGGC+AGG - chr5_2:64809968-64809987 None:intergenic 55.0%
CACCATACCTGACGTGGTTC+TGG + chr5_2:64810186-64810205 Msa0782120:intron 55.0%
GGTGGAGGCTTAGTCACTAG+CGG + chr5_2:64810832-64810851 Msa0782120:intron 55.0%
GAGGCTTAGTCACTAGCGGA+AGG + chr5_2:64810836-64810855 Msa0782120:intron 55.0%
! GAGAGAGTTGGGAGTGTGCA+TGG + chr5_2:64814023-64814042 Msa0782120:intron 55.0%
CAGTCCCCGTTGCACTTCCA+AGG - chr5_2:64809605-64809624 None:intergenic 60.0%
! CTTGGAAGTGCAACGGGGAC+TGG + chr5_2:64809603-64809622 Msa0782120:CDS 60.0%
! TGTTGCAAGGCGGATGGTGG+AGG + chr5_2:64810817-64810836 Msa0782120:intron 60.0%
GTTCACCTTAGGGCACGGTG+TGG + chr5_2:64813194-64813213 Msa0782120:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 64809454 64814395 64809454 ID=Msa0782120;Name=Msa0782120
chr5_2 mRNA 64809454 64814395 64809454 ID=Msa0782120-mRNA-1;Parent=Msa0782120;Name=Msa0782120-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.33|0.5|0.5|0.66|0.5|4|491|492
chr5_2 exon 64809454 64809732 64809454 ID=Msa0782120-mRNA-1:exon:19468;Parent=Msa0782120-mRNA-1
chr5_2 exon 64809860 64809905 64809860 ID=Msa0782120-mRNA-1:exon:19469;Parent=Msa0782120-mRNA-1
chr5_2 exon 64812725 64814010 64812725 ID=Msa0782120-mRNA-1:exon:19470;Parent=Msa0782120-mRNA-1
chr5_2 exon 64814037 64814395 64814037 ID=Msa0782120-mRNA-1:exon:19471;Parent=Msa0782120-mRNA-1
chr5_2 CDS 64809454 64809732 64809454 ID=Msa0782120-mRNA-1:cds;Parent=Msa0782120-mRNA-1
chr5_2 CDS 64809860 64809905 64809860 ID=Msa0782120-mRNA-1:cds;Parent=Msa0782120-mRNA-1
chr5_2 CDS 64812725 64813878 64812725 ID=Msa0782120-mRNA-1:cds;Parent=Msa0782120-mRNA-1
chr5_2 three_prime_UTR 64813879 64814010 64813879 ID=Msa0782120-mRNA-1:three_prime_utr;Parent=Msa0782120-mRNA-1
chr5_2 three_prime_UTR 64814037 64814395 64814037 ID=Msa0782120-mRNA-1:three_prime_utr;Parent=Msa0782120-mRNA-1
Gene Sequence

>Msa0782120

ATGTCTCTTGCAGTTGTGCTTGAGCATAGAAGTCTCTTGATTGTGTTCTTGAGCATAGGAAGACTCTTGCAAAGTGTGCTTGAGCAAAGGGAGTCTCTTGCTGTGTGCTTGAGCAATTGTAATCAGTTATTACATTCTAGTGAAATCTCCTTGGAAGTGCAACGGGGACTGGACTACTTCCAGATTGTGGAAGGAACCAATATAATTGCTTTGTGTTTCTTCTTCTCTTTCTCTGTTTTATTCCCCTGCGTTCAGATTATGTTTATCACTTCAGAAGCTTTTCAAATTATAGTGAGAATTGTCGTTGTTTCTAGAACCGCGTCAGGTGGAGAGAGCGAAGATAGCAAATGTTATTGTGCAAAATATGAGAAAAGTAAAGAAGCATGTATCGAGAAGCCTAAGAAGAGTTTCTCTTGCGGAAATCCAGAATGTGATGATTTGAAGGATATCGGCATCAAACCAGAATGTCCAGGTTCTAGAAAAGATAAAGATTTATGCGAGTTTAGTATGAGTTATGTAGACTGTACTAAATCAAAAGGAATTTATGGTCCAGCTAATCTTTGGTTTACAACGATCAAAAGTGAAACCAAAAAAGAAAGATTGAACATTTGGTTTTCATTTCCAACCAGCACAAAAAAGAAAGATGATGAAGAACCAAAAAACCAAGGAGTTGTTGGACTTGGATCTGGGAAGTTATCTTTGATAAAACAGTATCAACACATCACTCCCAAATTCTCTTATTATCTTCCACAATCTCGTCGCAAAGACGAAGAAGATATTAAAGCTATAAGCAAGTTCACCTTAGGGCACGGTGTGGAATTTTTGGAGAAGTTGAGCACTCCTTTAACAGAACAAGGTCATTCTTTGTGTGAAACTCGTTACTGCGTCCATCTTAAAAGTATTTCTGTAGGTGAAAGTGAAAATAACAAAATACCGATAAATGATGACACTCATGAAGCTCATAAGGTAATGATTATTGATTCAGGTCACACATTTACACATCTTAGTAAAGATATATTCGACAAATTTTTAGAAAAAGTAGAAAAAGAATATGGGAAAAAGGGAGAAAAGAAAGGCTGTGATTATCAAAATTGTTTCGAGAAAGATGGGAAAGTTGAGAAATTATTGAAGAAAATATCATTTGAGTTTCAAGATATAAAAATTGAGTTGAAGAGGGAGAACTTTTTAGATGATTTTTCTTATAAAGAACCCAACGAGGAGGAAAAACATTATATATGCTTAACAGTGCAAGGAGAAAATGAAAAGTATCATCAAAGGCTGCGTAACTATGGGAAGTCAGAGGAGGAGAAAAGACCGAATATACTTGGAAGTAGGGCACAGATGGATTTCTTGGTGGCTTTCAATCTTAAAAAAGGTATTAATAAAGTGTCTTTTGAGGATAAGAGTAAGCCATTGAAGAATTATCGGTCCTTTCAGAATAAGAATATGTGTGTATATATGCCATGCCATTTGGTATAA
Protein sequence

>Msa0782120

MSLAVVLEHRSLLIVFLSIGRLLQSVLEQRESLAVCLSNCNQLLHSSEISLEVQRGLDYFQIVEGTNIIALCFFFSFSVLFPCVQIMFITSEAFQIIVRIVVVSRTASGGESEDSKCYCAKYEKSKEACIEKPKKSFSCGNPECDDLKDIGIKPECPGSRKDKDLCEFSMSYVDCTKSKGIYGPANLWFTTIKSETKKERLNIWFSFPTSTKKKDDEEPKNQGVVGLGSGKLSLIKQYQHITPKFSYYLPQSRRKDEEDIKAISKFTLGHGVEFLEKLSTPLTEQGHSLCETRYCVHLKSISVGESENNKIPINDDTHEAHKVMIIDSGHTFTHLSKDIFDKFLEKVEKEYGKKGEKKGCDYQNCFEKDGKVEKLLKKISFEFQDIKIELKRENFLDDFSYKEPNEEEKHYICLTVQGENEKYHQRLRNYGKSEEEKRPNILGSRAQMDFLVAFNLKKGINKVSFEDKSKPLKNYRSFQNKNMCVYMPCHLV*