AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0682360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682360 MtrunA17_Chr4g0028501 40.104 192 106 6 19 205 20 207 1.09e-41 140
Msa0682360 MtrunA17_Chr3g0089081 35.385 195 113 8 20 201 70 264 1.18e-31 115
Msa0682360 MtrunA17_Chr3g0089091 34.872 195 114 8 20 201 79 273 1.26e-31 115
Msa0682360 MtrunA17_Chr6g0479301 36.715 207 105 11 18 200 76 280 9.82e-29 108
Msa0682360 MtrunA17_Chr2g0287541 30.890 191 123 6 16 200 3 190 5.72e-28 104
Msa0682360 MtrunA17_Chr1g0170111 36.181 199 101 10 18 194 102 296 1.31e-27 106
Msa0682360 MtrunA17_Chr2g0287471 36.207 174 100 7 26 190 163 334 5.92e-27 105
Msa0682360 MtrunA17_Chr2g0287571 31.694 183 116 6 15 191 8 187 4.94e-25 97.1
Msa0682360 MtrunA17_Chr5g0417341 33.498 203 114 11 19 201 27 228 5.22e-25 97.4
Msa0682360 MtrunA17_Chr8g0350611 33.168 202 109 8 20 201 104 299 4.32e-24 96.7
Msa0682360 MtrunA17_Chr6g0479341 31.863 204 115 10 18 200 69 269 1.13e-23 95.1
Msa0682360 MtrunA17_Chr5g0417371 30.045 223 135 12 1 203 6 227 1.44e-22 91.3
Msa0682360 MtrunA17_Chr5g0417321 31.707 205 113 12 19 201 27 226 4.65e-22 89.7
Msa0682360 MtrunA17_Chr5g0439761 31.959 194 119 9 21 201 21 214 8.64e-22 89.0
Msa0682360 MtrunA17_Chr6g0483091 31.553 206 121 10 2 188 7 211 2.53e-21 87.8
Msa0682360 MtrunA17_Chr8g0339941 33.663 202 107 12 19 201 415 608 3.63e-20 87.8
Msa0682360 MtrunA17_Chr2g0287561 37.719 114 64 5 85 194 27 137 3.17e-18 77.8
Msa0682360 MtrunA17_Chr8g0347611 32.979 188 90 8 19 178 42 221 1.14e-17 79.0
Msa0682360 MtrunA17_Chr5g0417381 27.064 218 139 12 3 202 12 227 1.26e-15 72.8
Msa0682360 MtrunA17_Chr2g0333421 32.836 134 70 8 77 203 8 128 3.30e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682360 AT2G02990.1 31.507 219 131 11 1 201 11 228 1.08e-20 86.7
Msa0682360 AT1G26820.1 29.091 220 136 12 3 203 4 222 3.27e-19 82.4
Msa0682360 AT2G39780.1 27.957 186 108 7 16 178 28 210 1.41e-18 81.6
Msa0682360 AT1G14220.1 30.151 199 121 10 7 188 15 212 3.11e-17 77.4
Msa0682360 AT1G14210.1 26.457 223 143 11 1 203 7 228 4.47e-14 68.9

Find 57 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTTTGATGTCATTT+AGG 0.156708 4_4:-43821968 None:intergenic
GTCTCATTTGTGCAACGATA+TGG 0.213688 4_4:-43821688 None:intergenic
AAGATCTTTGATGTCATTTA+GGG 0.233880 4_4:-43821967 None:intergenic
GTAATAGTCAAAGTTTATTT+TGG 0.236072 4_4:-43821551 None:intergenic
GTTATTGTTCTCTTTGTTCC+TGG 0.282679 4_4:+43821510 Msa0682360:CDS
CATTTAGGGCATACAAATCT+AGG 0.293125 4_4:-43821953 None:intergenic
CGGGAAACAACTCTGACTTT+TGG 0.314940 4_4:+43821865 Msa0682360:CDS
TGCCCGGGATTGATAAGATT+TGG 0.330378 4_4:+43821832 Msa0682360:CDS
TAACTTTATTTAATAACTTC+TGG 0.331114 4_4:-43822200 None:intergenic
GTAATTTGTTGGAAGAAGTT+AGG 0.359195 4_4:+43822102 Msa0682360:CDS
GTTAATAAAATTATATATGA+TGG 0.387686 4_4:+43822219 Msa0682360:three_prime_UTR
TATATAAGTTGTCCTAAAAC+TGG 0.405140 4_4:+43822149 Msa0682360:CDS
TACCAGCTTGATCCATTGCC+CGG 0.424603 4_4:+43821816 Msa0682360:intron
TCTCATTTGTGCAACGATAT+GGG 0.445684 4_4:-43821687 None:intergenic
GAGTTGTTTCCCGTTAGATT+CGG 0.449794 4_4:-43821855 None:intergenic
GTAATCGTATGGTGACATAT+TGG 0.450909 4_4:-43821919 None:intergenic
TTTGTGCAACGATATGGGTT+AGG 0.459135 4_4:-43821682 None:intergenic
AAGAGAACAATAACGACATA+AGG 0.465778 4_4:-43821501 None:intergenic
AACTTTAACACCTGGTGGAT+CGG 0.470033 4_4:+43821998 Msa0682360:CDS
CCTAAAACTGGTACAAATTG+TGG 0.472383 4_4:+43822161 Msa0682360:CDS
AAGATTTGGCCGAATCTAAC+GGG 0.475239 4_4:+43821846 Msa0682360:CDS
ATTACTATTGTGTGGGTGCA+TGG 0.475775 4_4:+43822248 Msa0682360:three_prime_UTR
ATCGAGAGTGCTATAAAGTT+AGG 0.477479 4_4:+43822035 Msa0682360:CDS
CTTAATAAAACTTTAACACC+TGG 0.479447 4_4:+43821990 Msa0682360:CDS
CGGCCAAATCTTATCAATCC+CGG 0.483167 4_4:-43821835 None:intergenic
GTTGGTTATTACTATTGTGT+GGG 0.494348 4_4:+43822241 Msa0682360:three_prime_UTR
CTAAATAAATATGGAGGAGA+CGG 0.501939 4_4:+43822312 Msa0682360:three_prime_UTR
ATAAAATTATATATGATGGT+TGG 0.504489 4_4:+43822223 Msa0682360:three_prime_UTR
GGTTGGTTATTACTATTGTG+TGG 0.512296 4_4:+43822240 Msa0682360:three_prime_UTR
ATCTTATCAATCCCGGGCAA+TGG 0.515207 4_4:-43821828 None:intergenic
ATTTCAGCATCCACGGATTG+TGG 0.518420 4_4:+43821641 Msa0682360:CDS
TCTGCTTCAATACCACCAAC+AGG 0.519484 4_4:+43822126 Msa0682360:CDS
TGTTCTGCGGCCACAATCCG+TGG 0.527412 4_4:-43821651 None:intergenic
TAAGATTTGGCCGAATCTAA+CGG 0.533520 4_4:+43821845 Msa0682360:CDS
CCTCAAAATTTCAGCATCCA+CGG 0.533572 4_4:+43821634 Msa0682360:CDS
CGTTGCACAAATGAGACACT+GGG 0.545417 4_4:+43821694 Msa0682360:CDS
GGCCAAATCTTATCAATCCC+GGG 0.549058 4_4:-43821834 None:intergenic
AATAAAACTTTAACACCTGG+TGG 0.549310 4_4:+43821993 Msa0682360:CDS
CAGTAGATGAGTGCAAGTCC+AGG 0.554250 4_4:-43821528 None:intergenic
AGGACAACTTATATACCTGT+TGG 0.562314 4_4:-43822141 None:intergenic
ATAAATATGGAGGAGACGGA+GGG 0.564155 4_4:+43822316 Msa0682360:three_prime_UTR
TCGTTGCACAAATGAGACAC+TGG 0.565148 4_4:+43821693 Msa0682360:CDS
GCTATAAAGTTAGGCACTAA+CGG 0.567596 4_4:+43822044 Msa0682360:CDS
AATAAATATGGAGGAGACGG+AGG 0.573129 4_4:+43822315 Msa0682360:three_prime_UTR
ACCAGCTTGATCCATTGCCC+GGG 0.575199 4_4:+43821817 Msa0682360:intron
ATTTAGGGCATACAAATCTA+GGG 0.575925 4_4:-43821952 None:intergenic
GATTGTGGCCGCAGAACAAG+AGG 0.588738 4_4:+43821656 Msa0682360:CDS
GCTTTCGTCGCCGATCCACC+AGG 0.597466 4_4:-43822008 None:intergenic
TGAGACACTGGGGCCGCATG+TGG 0.606262 4_4:+43821705 Msa0682360:CDS
ACAACTTATATACCTGTTGG+TGG 0.612648 4_4:-43822138 None:intergenic
GCAAGCTCGAAGTAATCGTA+TGG 0.614270 4_4:-43821930 None:intergenic
TTAGGCTTCCTCTTGTTCTG+CGG 0.625435 4_4:-43821664 None:intergenic
TCGAGATAGCATACCACATG+CGG 0.641657 4_4:-43821718 None:intergenic
GCTTACGAGTGGAAGATACA+TGG 0.644977 4_4:+43821888 Msa0682360:CDS
ATTACATGCTAGCAGAAACA+TGG 0.657545 4_4:+43821569 Msa0682360:CDS
TAAATATGGAGGAGACGGAG+GGG 0.657667 4_4:+43822317 Msa0682360:three_prime_UTR
GTTGCACAAATGAGACACTG+GGG 0.801643 4_4:+43821695 Msa0682360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAAAAAATAACAAA+AGG - chr4_4:43821468-43821487 None:intergenic 10.0%
!!! TTTGTTATTTTTTATTTCTT+TGG + chr4_4:43821468-43821487 Msa0682360:five_prime_UTR 10.0%
!!! ATTTTACTATTTTTATCTTA+TGG + chr4_4:43821754-43821773 Msa0682360:intron 10.0%
!! GTTAATAAAATTATATATGA+TGG + chr4_4:43822219-43822238 Msa0682360:three_prime_UTR 10.0%
!!! ATATGTTTTCTAAATAAATA+TGG + chr4_4:43822303-43822322 Msa0682360:three_prime_UTR 10.0%
!! AAGAAATAAAAAATAACAAA+AGG - chr4_4:43821468-43821487 None:intergenic 10.0%
!!! TTTGTTATTTTTTATTTCTT+TGG + chr4_4:43821468-43821487 Msa0682360:five_prime_UTR 10.0%
!!! ATTTTACTATTTTTATCTTA+TGG + chr4_4:43821754-43821773 Msa0682360:intron 10.0%
!! GTTAATAAAATTATATATGA+TGG + chr4_4:43822219-43822238 Msa0682360:three_prime_UTR 10.0%
!!! ATATGTTTTCTAAATAAATA+TGG + chr4_4:43822303-43822322 Msa0682360:three_prime_UTR 10.0%
!! ATAGTAAAATCAAAGATAAT+TGG - chr4_4:43821744-43821763 None:intergenic 15.0%
!!! TAAAAACTATAGTAATTTGT+TGG + chr4_4:43822091-43822110 Msa0682360:CDS 15.0%
!!! TAACTTTATTTAATAACTTC+TGG - chr4_4:43822203-43822222 None:intergenic 15.0%
!! ATAAAATTATATATGATGGT+TGG + chr4_4:43822223-43822242 Msa0682360:three_prime_UTR 15.0%
!! AAAAAATAACAAAAGGAAAT+TGG - chr4_4:43821461-43821480 None:intergenic 15.0%
!! ATAGTAAAATCAAAGATAAT+TGG - chr4_4:43821744-43821763 None:intergenic 15.0%
!!! TAAAAACTATAGTAATTTGT+TGG + chr4_4:43822091-43822110 Msa0682360:CDS 15.0%
!!! TAACTTTATTTAATAACTTC+TGG - chr4_4:43822203-43822222 None:intergenic 15.0%
!! ATAAAATTATATATGATGGT+TGG + chr4_4:43822223-43822242 Msa0682360:three_prime_UTR 15.0%
!!! GTAATAGTCAAAGTTTATTT+TGG - chr4_4:43821554-43821573 None:intergenic 20.0%
!!! TGAAATTTTGAGGTATATTA+GGG - chr4_4:43821627-43821646 None:intergenic 20.0%
!!! TGTTTTCTAAATAAATATGG+AGG + chr4_4:43822306-43822325 Msa0682360:three_prime_UTR 20.0%
!!! GTAATAGTCAAAGTTTATTT+TGG - chr4_4:43821554-43821573 None:intergenic 20.0%
!!! TGAAATTTTGAGGTATATTA+GGG - chr4_4:43821627-43821646 None:intergenic 20.0%
!!! TGTTTTCTAAATAAATATGG+AGG + chr4_4:43822306-43822325 Msa0682360:three_prime_UTR 20.0%
!! GATTTGTTTAGACAAAAAGT+TGG - chr4_4:43821598-43821617 None:intergenic 25.0%
!!! CTGAAATTTTGAGGTATATT+AGG - chr4_4:43821628-43821647 None:intergenic 25.0%
!!! CTATTTTTATCTTATGGTGT+TGG + chr4_4:43821760-43821779 Msa0682360:intron 25.0%
!! AAGATCTTTGATGTCATTTA+GGG - chr4_4:43821970-43821989 None:intergenic 25.0%
! CTTAATAAAACTTTAACACC+TGG + chr4_4:43821990-43822009 Msa0682360:CDS 25.0%
! TATATAAGTTGTCCTAAAAC+TGG + chr4_4:43822149-43822168 Msa0682360:CDS 25.0%
!! GATTTGTTTAGACAAAAAGT+TGG - chr4_4:43821598-43821617 None:intergenic 25.0%
!!! CTGAAATTTTGAGGTATATT+AGG - chr4_4:43821628-43821647 None:intergenic 25.0%
!!! CTATTTTTATCTTATGGTGT+TGG + chr4_4:43821760-43821779 Msa0682360:intron 25.0%
!! AAGATCTTTGATGTCATTTA+GGG - chr4_4:43821970-43821989 None:intergenic 25.0%
! CTTAATAAAACTTTAACACC+TGG + chr4_4:43821990-43822009 Msa0682360:CDS 25.0%
! TATATAAGTTGTCCTAAAAC+TGG + chr4_4:43822149-43822168 Msa0682360:CDS 25.0%
AAGAGAACAATAACGACATA+AGG - chr4_4:43821504-43821523 None:intergenic 30.0%
TTGTTTAGACAAAAAGTTGG+TGG - chr4_4:43821595-43821614 None:intergenic 30.0%
! ATTTAGGGCATACAAATCTA+GGG - chr4_4:43821955-43821974 None:intergenic 30.0%
! GAAGATCTTTGATGTCATTT+AGG - chr4_4:43821971-43821990 None:intergenic 30.0%
AATAAAACTTTAACACCTGG+TGG + chr4_4:43821993-43822012 Msa0682360:CDS 30.0%
!! GTAATTTGTTGGAAGAAGTT+AGG + chr4_4:43822102-43822121 Msa0682360:CDS 30.0%
GTTGGTTATTACTATTGTGT+GGG + chr4_4:43822241-43822260 Msa0682360:three_prime_UTR 30.0%
CTAAATAAATATGGAGGAGA+CGG + chr4_4:43822312-43822331 Msa0682360:three_prime_UTR 30.0%
AAGAGAACAATAACGACATA+AGG - chr4_4:43821504-43821523 None:intergenic 30.0%
TTGTTTAGACAAAAAGTTGG+TGG - chr4_4:43821595-43821614 None:intergenic 30.0%
! ATTTAGGGCATACAAATCTA+GGG - chr4_4:43821955-43821974 None:intergenic 30.0%
! GAAGATCTTTGATGTCATTT+AGG - chr4_4:43821971-43821990 None:intergenic 30.0%
AATAAAACTTTAACACCTGG+TGG + chr4_4:43821993-43822012 Msa0682360:CDS 30.0%
!! GTAATTTGTTGGAAGAAGTT+AGG + chr4_4:43822102-43822121 Msa0682360:CDS 30.0%
GTTGGTTATTACTATTGTGT+GGG + chr4_4:43822241-43822260 Msa0682360:three_prime_UTR 30.0%
CTAAATAAATATGGAGGAGA+CGG + chr4_4:43822312-43822331 Msa0682360:three_prime_UTR 30.0%
GTTATTGTTCTCTTTGTTCC+TGG + chr4_4:43821510-43821529 Msa0682360:CDS 35.0%
ATTACATGCTAGCAGAAACA+TGG + chr4_4:43821569-43821588 Msa0682360:CDS 35.0%
TCTCATTTGTGCAACGATAT+GGG - chr4_4:43821690-43821709 None:intergenic 35.0%
TAAGATTTGGCCGAATCTAA+CGG + chr4_4:43821845-43821864 Msa0682360:CDS 35.0%
GTAATCGTATGGTGACATAT+TGG - chr4_4:43821922-43821941 None:intergenic 35.0%
! CATTTAGGGCATACAAATCT+AGG - chr4_4:43821956-43821975 None:intergenic 35.0%
!! ATCGAGAGTGCTATAAAGTT+AGG + chr4_4:43822035-43822054 Msa0682360:CDS 35.0%
GCTATAAAGTTAGGCACTAA+CGG + chr4_4:43822044-43822063 Msa0682360:CDS 35.0%
! TTTTTATGACAACGAAGCTG+AGG - chr4_4:43822077-43822096 None:intergenic 35.0%
ACAACTTATATACCTGTTGG+TGG - chr4_4:43822141-43822160 None:intergenic 35.0%
AGGACAACTTATATACCTGT+TGG - chr4_4:43822144-43822163 None:intergenic 35.0%
! CCACAATTTGTACCAGTTTT+AGG - chr4_4:43822164-43822183 None:intergenic 35.0%
CCTAAAACTGGTACAAATTG+TGG + chr4_4:43822161-43822180 Msa0682360:CDS 35.0%
! GGTTGGTTATTACTATTGTG+TGG + chr4_4:43822240-43822259 Msa0682360:three_prime_UTR 35.0%
GTTATTGTTCTCTTTGTTCC+TGG + chr4_4:43821510-43821529 Msa0682360:CDS 35.0%
ATTACATGCTAGCAGAAACA+TGG + chr4_4:43821569-43821588 Msa0682360:CDS 35.0%
TCTCATTTGTGCAACGATAT+GGG - chr4_4:43821690-43821709 None:intergenic 35.0%
TAAGATTTGGCCGAATCTAA+CGG + chr4_4:43821845-43821864 Msa0682360:CDS 35.0%
GTAATCGTATGGTGACATAT+TGG - chr4_4:43821922-43821941 None:intergenic 35.0%
! CATTTAGGGCATACAAATCT+AGG - chr4_4:43821956-43821975 None:intergenic 35.0%
!! ATCGAGAGTGCTATAAAGTT+AGG + chr4_4:43822035-43822054 Msa0682360:CDS 35.0%
GCTATAAAGTTAGGCACTAA+CGG + chr4_4:43822044-43822063 Msa0682360:CDS 35.0%
! TTTTTATGACAACGAAGCTG+AGG - chr4_4:43822077-43822096 None:intergenic 35.0%
ACAACTTATATACCTGTTGG+TGG - chr4_4:43822141-43822160 None:intergenic 35.0%
AGGACAACTTATATACCTGT+TGG - chr4_4:43822144-43822163 None:intergenic 35.0%
! CCACAATTTGTACCAGTTTT+AGG - chr4_4:43822164-43822183 None:intergenic 35.0%
CCTAAAACTGGTACAAATTG+TGG + chr4_4:43822161-43822180 Msa0682360:CDS 35.0%
! GGTTGGTTATTACTATTGTG+TGG + chr4_4:43822240-43822259 Msa0682360:three_prime_UTR 35.0%
CCTCAAAATTTCAGCATCCA+CGG + chr4_4:43821634-43821653 Msa0682360:CDS 40.0%
TTTGTGCAACGATATGGGTT+AGG - chr4_4:43821685-43821704 None:intergenic 40.0%
GTCTCATTTGTGCAACGATA+TGG - chr4_4:43821691-43821710 None:intergenic 40.0%
AAGATTTGGCCGAATCTAAC+GGG + chr4_4:43821846-43821865 Msa0682360:CDS 40.0%
! GAGTTGTTTCCCGTTAGATT+CGG - chr4_4:43821858-43821877 None:intergenic 40.0%
! GGGAAACAACTCTGACTTTT+GGG + chr4_4:43821866-43821885 Msa0682360:CDS 40.0%
! AACTTTAACACCTGGTGGAT+CGG + chr4_4:43821998-43822017 Msa0682360:CDS 40.0%
ATTACTATTGTGTGGGTGCA+TGG + chr4_4:43822248-43822267 Msa0682360:three_prime_UTR 40.0%
AATAAATATGGAGGAGACGG+AGG + chr4_4:43822315-43822334 Msa0682360:three_prime_UTR 40.0%
ATAAATATGGAGGAGACGGA+GGG + chr4_4:43822316-43822335 Msa0682360:three_prime_UTR 40.0%
CCTCAAAATTTCAGCATCCA+CGG + chr4_4:43821634-43821653 Msa0682360:CDS 40.0%
TTTGTGCAACGATATGGGTT+AGG - chr4_4:43821685-43821704 None:intergenic 40.0%
GTCTCATTTGTGCAACGATA+TGG - chr4_4:43821691-43821710 None:intergenic 40.0%
AAGATTTGGCCGAATCTAAC+GGG + chr4_4:43821846-43821865 Msa0682360:CDS 40.0%
! GAGTTGTTTCCCGTTAGATT+CGG - chr4_4:43821858-43821877 None:intergenic 40.0%
! GGGAAACAACTCTGACTTTT+GGG + chr4_4:43821866-43821885 Msa0682360:CDS 40.0%
! AACTTTAACACCTGGTGGAT+CGG + chr4_4:43821998-43822017 Msa0682360:CDS 40.0%
ATTACTATTGTGTGGGTGCA+TGG + chr4_4:43822248-43822267 Msa0682360:three_prime_UTR 40.0%
AATAAATATGGAGGAGACGG+AGG + chr4_4:43822315-43822334 Msa0682360:three_prime_UTR 40.0%
ATAAATATGGAGGAGACGGA+GGG + chr4_4:43822316-43822335 Msa0682360:three_prime_UTR 40.0%
! CCGTGGATGCTGAAATTTTG+AGG - chr4_4:43821637-43821656 None:intergenic 45.0%
! ATTTCAGCATCCACGGATTG+TGG + chr4_4:43821641-43821660 Msa0682360:CDS 45.0%
TTAGGCTTCCTCTTGTTCTG+CGG - chr4_4:43821667-43821686 None:intergenic 45.0%
TCGTTGCACAAATGAGACAC+TGG + chr4_4:43821693-43821712 Msa0682360:CDS 45.0%
CGTTGCACAAATGAGACACT+GGG + chr4_4:43821694-43821713 Msa0682360:CDS 45.0%
GTTGCACAAATGAGACACTG+GGG + chr4_4:43821695-43821714 Msa0682360:CDS 45.0%
TCGAGATAGCATACCACATG+CGG - chr4_4:43821721-43821740 None:intergenic 45.0%
ATCTTATCAATCCCGGGCAA+TGG - chr4_4:43821831-43821850 None:intergenic 45.0%
TGCCCGGGATTGATAAGATT+TGG + chr4_4:43821832-43821851 Msa0682360:CDS 45.0%
GGCCAAATCTTATCAATCCC+GGG - chr4_4:43821837-43821856 None:intergenic 45.0%
CGGCCAAATCTTATCAATCC+CGG - chr4_4:43821838-43821857 None:intergenic 45.0%
! CGGGAAACAACTCTGACTTT+TGG + chr4_4:43821865-43821884 Msa0682360:CDS 45.0%
GCTTACGAGTGGAAGATACA+TGG + chr4_4:43821888-43821907 Msa0682360:CDS 45.0%
GCAAGCTCGAAGTAATCGTA+TGG - chr4_4:43821933-43821952 None:intergenic 45.0%
TCTGCTTCAATACCACCAAC+AGG + chr4_4:43822126-43822145 Msa0682360:CDS 45.0%
TAAATATGGAGGAGACGGAG+GGG + chr4_4:43822317-43822336 Msa0682360:three_prime_UTR 45.0%
! CCGTGGATGCTGAAATTTTG+AGG - chr4_4:43821637-43821656 None:intergenic 45.0%
! ATTTCAGCATCCACGGATTG+TGG + chr4_4:43821641-43821660 Msa0682360:CDS 45.0%
TTAGGCTTCCTCTTGTTCTG+CGG - chr4_4:43821667-43821686 None:intergenic 45.0%
TCGTTGCACAAATGAGACAC+TGG + chr4_4:43821693-43821712 Msa0682360:CDS 45.0%
CGTTGCACAAATGAGACACT+GGG + chr4_4:43821694-43821713 Msa0682360:CDS 45.0%
GTTGCACAAATGAGACACTG+GGG + chr4_4:43821695-43821714 Msa0682360:CDS 45.0%
TCGAGATAGCATACCACATG+CGG - chr4_4:43821721-43821740 None:intergenic 45.0%
ATCTTATCAATCCCGGGCAA+TGG - chr4_4:43821831-43821850 None:intergenic 45.0%
TGCCCGGGATTGATAAGATT+TGG + chr4_4:43821832-43821851 Msa0682360:CDS 45.0%
GGCCAAATCTTATCAATCCC+GGG - chr4_4:43821837-43821856 None:intergenic 45.0%
CGGCCAAATCTTATCAATCC+CGG - chr4_4:43821838-43821857 None:intergenic 45.0%
! CGGGAAACAACTCTGACTTT+TGG + chr4_4:43821865-43821884 Msa0682360:CDS 45.0%
GCTTACGAGTGGAAGATACA+TGG + chr4_4:43821888-43821907 Msa0682360:CDS 45.0%
GCAAGCTCGAAGTAATCGTA+TGG - chr4_4:43821933-43821952 None:intergenic 45.0%
TCTGCTTCAATACCACCAAC+AGG + chr4_4:43822126-43822145 Msa0682360:CDS 45.0%
TAAATATGGAGGAGACGGAG+GGG + chr4_4:43822317-43822336 Msa0682360:three_prime_UTR 45.0%
CAGTAGATGAGTGCAAGTCC+AGG - chr4_4:43821531-43821550 None:intergenic 50.0%
TACCAGCTTGATCCATTGCC+CGG + chr4_4:43821816-43821835 Msa0682360:intron 50.0%
! CTGACTTTTGGGCTTACGAG+TGG + chr4_4:43821877-43821896 Msa0682360:CDS 50.0%
CAGTAGATGAGTGCAAGTCC+AGG - chr4_4:43821531-43821550 None:intergenic 50.0%
TACCAGCTTGATCCATTGCC+CGG + chr4_4:43821816-43821835 Msa0682360:intron 50.0%
! CTGACTTTTGGGCTTACGAG+TGG + chr4_4:43821877-43821896 Msa0682360:CDS 50.0%
GATTGTGGCCGCAGAACAAG+AGG + chr4_4:43821656-43821675 Msa0682360:CDS 55.0%
ACCAGCTTGATCCATTGCCC+GGG + chr4_4:43821817-43821836 Msa0682360:intron 55.0%
GATTGTGGCCGCAGAACAAG+AGG + chr4_4:43821656-43821675 Msa0682360:CDS 55.0%
ACCAGCTTGATCCATTGCCC+GGG + chr4_4:43821817-43821836 Msa0682360:intron 55.0%
TGTTCTGCGGCCACAATCCG+TGG - chr4_4:43821654-43821673 None:intergenic 60.0%
TCCCGGGCAATGGATCAAGC+TGG - chr4_4:43821821-43821840 None:intergenic 60.0%
TGTTCTGCGGCCACAATCCG+TGG - chr4_4:43821654-43821673 None:intergenic 60.0%
TCCCGGGCAATGGATCAAGC+TGG - chr4_4:43821821-43821840 None:intergenic 60.0%
TGAGACACTGGGGCCGCATG+TGG + chr4_4:43821705-43821724 Msa0682360:CDS 65.0%
GCTTTCGTCGCCGATCCACC+AGG - chr4_4:43822011-43822030 None:intergenic 65.0%
TGAGACACTGGGGCCGCATG+TGG + chr4_4:43821705-43821724 Msa0682360:CDS 65.0%
GCTTTCGTCGCCGATCCACC+AGG - chr4_4:43822011-43822030 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 43821460 43822360 43821460 ID=Msa0682360;Name=Msa0682360
chr4_4 mRNA 43821460 43822360 43821460 ID=Msa0682360-mRNA-1;Parent=Msa0682360;Name=Msa0682360-mRNA-1;_AED=0.23;_eAED=0.23;_QI=45|1|1|1|1|1|2|143|205
chr4_4 exon 43821460 43821726 43821460 ID=Msa0682360-mRNA-1:exon:10627;Parent=Msa0682360-mRNA-1
chr4_4 exon 43821822 43822360 43821822 ID=Msa0682360-mRNA-1:exon:10628;Parent=Msa0682360-mRNA-1
chr4_4 five_prime_UTR 43821460 43821504 43821460 ID=Msa0682360-mRNA-1:five_prime_utr;Parent=Msa0682360-mRNA-1
chr4_4 CDS 43821505 43821726 43821505 ID=Msa0682360-mRNA-1:cds;Parent=Msa0682360-mRNA-1
chr4_4 CDS 43821822 43822217 43821822 ID=Msa0682360-mRNA-1:cds;Parent=Msa0682360-mRNA-1
chr4_4 three_prime_UTR 43822218 43822360 43822218 ID=Msa0682360-mRNA-1:three_prime_utr;Parent=Msa0682360-mRNA-1
Gene Sequence

>Msa0682360

ATGTCGTTATTGTTCTCTTTGTTCCTGGACTTGCACTCATCTACTGCCAAAATAAACTTTGACTATTACATGCTAGCAGAAACATGGCCACCAACTTTTTGTCTAAACAAATCATGTGTCCCTAATATACCTCAAAATTTCAGCATCCACGGATTGTGGCCGCAGAACAAGAGGAAGCCTAACCCATATCGTTGCACAAATGAGACACTGGGGCCGCATGTGCTTGATCCATTGCCCGGGATTGATAAGATTTGGCCGAATCTAACGGGAAACAACTCTGACTTTTGGGCTTACGAGTGGAAGATACATGGTACTTGCTCCAATATGTCACCATACGATTACTTCGAGCTTGCCCTAGATTTGTATGCCCTAAATGACATCAAAGATCTTCTTAATAAAACTTTAACACCTGGTGGATCGGCGACGAAAGCTGCTATCGAGAGTGCTATAAAGTTAGGCACTAACGGATCTAAACCTCAGCTTCGTTGTCATAAAAACTATAGTAATTTGTTGGAAGAAGTTAGGCTCTGCTTCAATACCACCAACAGGTATATAAGTTGTCCTAAAACTGGTACAAATTGTGGAGCTACAATACATTTACCAGAAGTTATTAAATAA
Protein sequence

>Msa0682360

MSLLFSLFLDLHSSTAKINFDYYMLAETWPPTFCLNKSCVPNIPQNFSIHGLWPQNKRKPNPYRCTNETLGPHVLDPLPGIDKIWPNLTGNNSDFWAYEWKIHGTCSNMSPYDYFELALDLYALNDIKDLLNKTLTPGGSATKAAIESAIKLGTNGSKPQLRCHKNYSNLLEEVRLCFNTTNRYISCPKTGTNCGATIHLPEVIK*