AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0819790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819790 MtrunA17_Chr2g0310831 30.204 245 126 10 259 499 225 428 1.12e-13 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819790 AT5G37540.1 27.536 276 157 15 182 439 137 387 5.95e-14 74.3

Find 86 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGATAATAAGAGAATT+TGG 0.156886 5_3:-56585539 None:intergenic
GAATGACCTTGTTCTGTTAA+AGG 0.180983 5_3:-56585652 None:intergenic
GACCGAATATACTTGGAATT+AGG 0.194702 5_3:+56586124 Msa0819790:CDS
TGATAGTACTAAAGTTAATT+TGG 0.228281 5_3:+56586491 Msa0819790:three_prime_UTR
AAAGTATTATTATTGATATA+AGG 0.241257 5_3:-56586469 None:intergenic
ACTAAATCAAAAGGAATTTA+TGG 0.245370 5_3:+56585337 Msa0819790:CDS
TTTCTAGAACCTGGACATTC+TGG 0.270492 5_3:-56585271 None:intergenic
CCGCAAGAGAAACTCTTCTT+AGG 0.279122 5_3:-56585208 None:intergenic
CATCAAAGGCTGCGTAACTA+TGG 0.292316 5_3:+56586081 Msa0819790:CDS
TAGGGCACAGATGGATTTCT+TGG 0.297891 5_3:+56586143 Msa0819790:CDS
ATGCCATGCCATTTGGTATA+AGG 0.301822 5_3:+56586271 Msa0819790:three_prime_UTR
AAGGTAATGATTATTGATTC+AGG 0.310716 5_3:+56585775 Msa0819790:CDS
TTTATGTAGAAATGTTCATT+AGG 0.312982 5_3:+56586593 Msa0819790:three_prime_UTR
TCCTTCAAATCATCACATTC+TGG 0.314277 5_3:-56585235 None:intergenic
TTTATGGTCCAGCTAATCTT+TGG 0.343745 5_3:+56585353 Msa0819790:CDS
GGAGTTGTTGGACTTGGATC+TGG 0.346598 5_3:+56585478 Msa0819790:CDS
TATTGGTTCCTTCCACAATC+TGG 0.350819 5_3:-56582089 None:intergenic
AAGACACAAAGCAATTATAT+TGG 0.354925 5_3:-56582106 None:intergenic
ACCGAATATACTTGGAATTA+GGG 0.357393 5_3:+56586125 Msa0819790:CDS
CTTGGAAGTGCAACGGGGAC+TGG 0.360881 5_3:+56582059 Msa0819790:CDS
GGAAGATAATAAGAGAATTT+GGG 0.362567 5_3:-56585538 None:intergenic
AAGTATTATTATTGATATAA+GGG 0.372276 5_3:-56586468 None:intergenic
GCTATAAGCAAGTTCACCTT+AGG 0.379444 5_3:+56585595 Msa0819790:CDS
AATCTCCTTGGAAGTGCAAC+GGG 0.380874 5_3:+56582053 Msa0819790:CDS
TGTAATTATACTTTATGATA+AGG 0.383859 5_3:-56586791 None:intergenic
CAAAATTGTTTCGAGAAAGA+TGG 0.384032 5_3:+56585898 Msa0819790:CDS
AGGACCGATAATTCTTCAAT+TGG 0.390609 5_3:-56586222 None:intergenic
TTATGTAGAAATGTTCATTA+GGG 0.391161 5_3:+56586594 Msa0819790:three_prime_UTR
TAAGCCAATTGAAGAATTAT+CGG 0.394355 5_3:+56586218 Msa0819790:CDS
GCCCTAATTCCAAGTATATT+CGG 0.401253 5_3:-56586126 None:intergenic
AACCAAGGAGTTGTTGGACT+TGG 0.403721 5_3:+56585472 Msa0819790:CDS
GAGAGAGTTGGGAGTGTGCA+TGG 0.419319 5_3:+56586436 Msa0819790:intron
TCCAGAATGTGATGATTTGA+AGG 0.431291 5_3:+56585234 Msa0819790:CDS
AATTTGAAACTGCAGCAGTT+TGG 0.436282 5_3:-56582304 None:intergenic
TGTGATGATTTGAAGGATAT+CGG 0.438665 5_3:+56585241 Msa0819790:CDS
GCAAAGTGTGCTTGAGCAAA+GGG 0.446414 5_3:+56581978 Msa0819790:CDS
TTATTAACTCCAAATTAACT+TGG 0.454252 5_3:-56586540 None:intergenic
GGCACAGATGGATTTCTTGG+TGG 0.455595 5_3:+56586146 Msa0819790:CDS
TGACACTCGTGATGCTCATA+AGG 0.475939 5_3:+56585756 Msa0819790:CDS
TGTATATATGCCATGCCATT+TGG 0.479150 5_3:+56586264 Msa0819790:three_prime_UTR
GTTGTAAACCAAAGATTAGC+TGG 0.481387 5_3:-56585361 None:intergenic
TCACGAGTGTCATCATTTAT+CGG 0.482679 5_3:-56585745 None:intergenic
GTAGACTGTACTAAATCAAA+AGG 0.484682 5_3:+56585328 Msa0819790:CDS
TCGTCTTTGCGACGAGATTG+TGG 0.487130 5_3:-56585559 None:intergenic
ATCCAAGTCCAACAACTCCT+TGG 0.491502 5_3:-56585474 None:intergenic
AAAATTGTTTCGAGAAAGAT+GGG 0.497335 5_3:+56585899 Msa0819790:CDS
GTTGTTTCTAGAACCGCGTC+AGG 0.497720 5_3:+56582342 Msa0819790:CDS
GAGAAAAGACCGAATATACT+TGG 0.500599 5_3:+56586117 Msa0819790:CDS
ACTTGGAATTAGGGCACAGA+TGG 0.506032 5_3:+56586134 Msa0819790:CDS
ACATTCTAGTGAAATCTCCT+TGG 0.509243 5_3:+56582041 Msa0819790:CDS
TGCAAAGTGTGCTTGAGCAA+AGG 0.522732 5_3:+56581977 Msa0819790:CDS
TATAGCAGCCTTATACCAAA+TGG 0.522877 5_3:-56586279 None:intergenic
TTTGGAGTTAATAATACCAT+GGG 0.526759 5_3:+56586549 Msa0819790:three_prime_UTR
GAGTTGTTGGACTTGGATCT+GGG 0.528801 5_3:+56585479 Msa0819790:CDS
ATCAAAGGCTGCGTAACTAT+GGG 0.545015 5_3:+56586082 Msa0819790:CDS
CAGTCCCCGTTGCACTTCCA+AGG 0.547760 5_3:-56582058 None:intergenic
GATAAACATAATCTGAACGC+AGG 0.551270 5_3:-56582156 None:intergenic
ATTTGGAGTTAATAATACCA+TGG 0.556947 5_3:+56586548 Msa0819790:three_prime_UTR
TATATATGCTTAACAGTGCA+AGG 0.561224 5_3:+56586042 Msa0819790:CDS
AAATTCCACACCGTGCCCTA+AGG 0.561293 5_3:-56585611 None:intergenic
ACACATATTCTTATTCTGAA+AGG 0.569596 5_3:-56586242 None:intergenic
TTGATTGTGTTCTTGAGCAT+AGG 0.571289 5_3:+56581946 Msa0819790:CDS
GGCATCAAACCAGAATGTCC+AGG 0.585165 5_3:+56585262 Msa0819790:CDS
AAATCTCCTTGGAAGTGCAA+CGG 0.586573 5_3:+56582052 Msa0819790:CDS
CATCTTAAAAGTATTTCTGT+AGG 0.587267 5_3:+56585700 Msa0819790:CDS
ACTGGACTACTTCCAGATTG+TGG 0.590531 5_3:+56582077 Msa0819790:CDS
CTATAAGCAAGTTCACCTTA+GGG 0.591792 5_3:+56585596 Msa0819790:CDS
CAGCCTTATACCAAATGGCA+TGG 0.599563 5_3:-56586274 None:intergenic
AGCACTCCTTTAACAGAACA+AGG 0.603395 5_3:+56585646 Msa0819790:CDS
TTCTTGACACACATGACTAG+AGG 0.604107 5_3:-56586305 None:intergenic
GACTACTTCCAGATTGTGGA+AGG 0.605667 5_3:+56582081 Msa0819790:CDS
AAAATGAAAAGTATCATCAA+AGG 0.605993 5_3:+56586067 Msa0819790:CDS
AGCAAGTTCACCTTAGGGCA+CGG 0.611602 5_3:+56585601 Msa0819790:CDS
ATAAACATAATCTGAACGCA+GGG 0.615500 5_3:-56582155 None:intergenic
TTGGAACGACAAAATTGTGA+AGG 0.619934 5_3:-56582285 None:intergenic
CTCCTAAAAGTTGAACACAC+TGG 0.634684 5_3:-56582209 None:intergenic
TCAGTTGTTAGAATGACCCA+TGG 0.639074 5_3:-56586565 None:intergenic
TTATAAAGAACCCAACGAGG+AGG 0.660088 5_3:+56586011 Msa0819790:CDS
CCTAAGAAGAGTTTCTCTTG+CGG 0.660827 5_3:+56585208 Msa0819790:CDS
GCGTAACTATGGGAAGTCAG+AGG 0.678639 5_3:+56586092 Msa0819790:CDS
TAACTATGGGAAGTCAGAGG+AGG 0.678721 5_3:+56586095 Msa0819790:CDS
TGTCAAGAATAATAAATGTG+TGG 0.688701 5_3:+56586319 Msa0819790:three_prime_UTR
GTTCACCTTAGGGCACGGTG+TGG 0.689834 5_3:+56585606 Msa0819790:CDS
TTCTTATAAAGAACCCAACG+AGG 0.696416 5_3:+56586008 Msa0819790:CDS
ATCTCCTTGGAAGTGCAACG+GGG 0.700856 5_3:+56582054 Msa0819790:CDS
TAAACATAATCTGAACGCAG+GGG 0.725156 5_3:-56582154 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATAAAATAAATAA+AGG - chr5_3:56584812-56584831 None:intergenic 0.0%
!! AATAAAAAAAAATGAGAAAA+AGG + chr5_3:56583717-56583736 Msa0819790:intron 10.0%
!! AATTCTTAAATAAAAACATT+TGG + chr5_3:56584342-56584361 Msa0819790:intron 10.0%
!!! TTTTTTTTTTATATTAAAGG+TGG + chr5_3:56585118-56585137 Msa0819790:intron 10.0%
!! AAGTATTATTATTGATATAA+GGG - chr5_3:56586471-56586490 None:intergenic 10.0%
!!! AAAGTATTATTATTGATATA+AGG - chr5_3:56586472-56586491 None:intergenic 10.0%
!! TAACATTAATTCATGATATT+TGG + chr5_3:56583543-56583562 Msa0819790:intron 15.0%
!! AATCAAAATGTAATATACAT+TGG - chr5_3:56583984-56584003 None:intergenic 15.0%
!!! AATGTATATTACATTTTGAT+TGG + chr5_3:56583983-56584002 Msa0819790:intron 15.0%
!!! CTTTTATAGAAAAATACTAA+CGG + chr5_3:56584431-56584450 Msa0819790:intron 15.0%
!!! TGACACTTTTTTTTTTTTTT+TGG - chr5_3:56584658-56584677 None:intergenic 15.0%
!!! AAAAAAAAAAAAGTGTCATT+AGG + chr5_3:56584660-56584679 Msa0819790:intron 15.0%
!!! AAAAAAAAAAAGTGTCATTA+GGG + chr5_3:56584661-56584680 Msa0819790:intron 15.0%
!! AAAAAGTAGAAAAAGAATAT+GGG + chr5_3:56585845-56585864 Msa0819790:CDS 15.0%
!!! AAAGACAATTTAAAGTTTTA+AGG + chr5_3:56586690-56586709 Msa0819790:three_prime_UTR 15.0%
!!! AAGACAATTTAAAGTTTTAA+GGG + chr5_3:56586691-56586710 Msa0819790:three_prime_UTR 15.0%
!!! CTTTTTGATTTGATTTTTCT+TGG - chr5_3:56582613-56582632 None:intergenic 20.0%
!! ACAAAATTGAACAATCAAAA+TGG + chr5_3:56582752-56582771 Msa0819790:intron 20.0%
!! CAAAATTGAACAATCAAAAT+GGG + chr5_3:56582753-56582772 Msa0819790:intron 20.0%
!! CTGAATATCCTTAAATTTAT+CGG - chr5_3:56582802-56582821 None:intergenic 20.0%
!! TGAACACCTAAATATAATTA+AGG + chr5_3:56582889-56582908 Msa0819790:intron 20.0%
!! ATCTTGCCTTAATTATATTT+AGG - chr5_3:56582898-56582917 None:intergenic 20.0%
!! TAACATTAATTCACGATATT+TGG - chr5_3:56583201-56583220 None:intergenic 20.0%
!!! TCTTTATTTTTTTTCCGTTT+CGG - chr5_3:56583775-56583794 None:intergenic 20.0%
!! CGGAAAAAAAATAAAGATAA+AGG + chr5_3:56583778-56583797 Msa0819790:intron 20.0%
!! CTTATTTATTTGTAACACTT+TGG - chr5_3:56583805-56583824 None:intergenic 20.0%
!! TATAGACAAGATTACATTAT+AGG - chr5_3:56583836-56583855 None:intergenic 20.0%
!!! TATTTTCAAAAATATCTACG+TGG + chr5_3:56584089-56584108 Msa0819790:intron 20.0%
!! TGCAAGTAATATAAATTAGA+TGG + chr5_3:56584198-56584217 Msa0819790:intron 20.0%
!! AATATAAATTAGATGGTACA+AGG + chr5_3:56584205-56584224 Msa0819790:intron 20.0%
!! GATTGAAATGTTCATTTATT+TGG + chr5_3:56584401-56584420 Msa0819790:intron 20.0%
!! CCAAAATTGTCTTATAAAAA+GGG - chr5_3:56584754-56584773 None:intergenic 20.0%
!! ACCAAAATTGTCTTATAAAA+AGG - chr5_3:56584755-56584774 None:intergenic 20.0%
!!! TAAAATAAATAAAGGTATGC+TGG - chr5_3:56584804-56584823 None:intergenic 20.0%
!! ACTAAATCAAAAGGAATTTA+TGG + chr5_3:56585337-56585356 Msa0819790:CDS 20.0%
!!! AATGTTCAATCTTTCTTTTT+TGG - chr5_3:56585401-56585420 None:intergenic 20.0%
!! AAAAAGAAAGATTGAACATT+TGG + chr5_3:56585401-56585420 Msa0819790:CDS 20.0%
!! GAAAAAGTAGAAAAAGAATA+TGG + chr5_3:56585844-56585863 Msa0819790:CDS 20.0%
!!! ATATAAAAATTGAGTTGAAG+AGG + chr5_3:56585965-56585984 Msa0819790:CDS 20.0%
!!! TATAAAAATTGAGTTGAAGA+GGG + chr5_3:56585966-56585985 Msa0819790:CDS 20.0%
!!! AAAATGAAAAGTATCATCAA+AGG + chr5_3:56586067-56586086 Msa0819790:CDS 20.0%
!!! TATTAATAAAGTGTCTTTTG+AGG + chr5_3:56586188-56586207 Msa0819790:CDS 20.0%
!! TGATAGTACTAAAGTTAATT+TGG + chr5_3:56586491-56586510 Msa0819790:three_prime_UTR 20.0%
!!! TATTTTACTCCAAGTTAATT+TGG + chr5_3:56586531-56586550 Msa0819790:three_prime_UTR 20.0%
!! TTATTAACTCCAAATTAACT+TGG - chr5_3:56586543-56586562 None:intergenic 20.0%
!! TTTATGTAGAAATGTTCATT+AGG + chr5_3:56586593-56586612 Msa0819790:three_prime_UTR 20.0%
!! TTATGTAGAAATGTTCATTA+GGG + chr5_3:56586594-56586613 Msa0819790:three_prime_UTR 20.0%
!!! AGACAATTTAAAGTTTTAAG+GGG + chr5_3:56586692-56586711 Msa0819790:three_prime_UTR 20.0%
! AAGACACAAAGCAATTATAT+TGG - chr5_3:56582109-56582128 None:intergenic 25.0%
! TAGCAATCCTTAAAGTATAA+CGG + chr5_3:56582550-56582569 Msa0819790:intron 25.0%
! GAATGTAACCGATAAATTTA+AGG + chr5_3:56582791-56582810 Msa0819790:intron 25.0%
! ATTAATTTACACTACAAGAC+TGG - chr5_3:56582863-56582882 None:intergenic 25.0%
! AATTAAGGCAAGATTACATT+AGG + chr5_3:56582904-56582923 Msa0819790:intron 25.0%
! ATTAAGGCAAGATTACATTA+GGG + chr5_3:56582905-56582924 Msa0819790:intron 25.0%
!!! ATCTTTATTTTTTCCCGTTT+AGG + chr5_3:56582967-56582986 Msa0819790:intron 25.0%
!!! TGTGATTTTAAGAGAGATAA+AGG - chr5_3:56582994-56583013 None:intergenic 25.0%
! AACATCTTCATCATATTTCT+AGG - chr5_3:56583640-56583659 None:intergenic 25.0%
! TGTGTTTAAAAGAAGATTTG+AGG - chr5_3:56583514-56583533 None:intergenic 25.0%
! TTGCAACATTTGTGAAATTA+AGG - chr5_3:56583263-56583282 None:intergenic 25.0%
! TTAATTTCACAAATGTTGCA+AGG + chr5_3:56583262-56583281 Msa0819790:intron 25.0%
! TACAAAAGTTAAGATTGTTC+TGG + chr5_3:56583326-56583345 Msa0819790:intron 25.0%
!! ATACTAAATCTCTTTTCTAC+AGG + chr5_3:56583480-56583499 Msa0819790:intron 25.0%
!! TACTAAATCTCTTTTCTACA+GGG + chr5_3:56583481-56583500 Msa0819790:intron 25.0%
! TGTGTTTAAAAGAAGATTTG+AGG + chr5_3:56583514-56583533 Msa0819790:intron 25.0%
!!! CTTTATTTTTTTTCCGTTTC+GGG - chr5_3:56583774-56583793 None:intergenic 25.0%
!!! TAGAAAAAACCCACTTTTAT+AGG + chr5_3:56584053-56584072 Msa0819790:intron 25.0%
! AGAAACTAACCTATAAAAGT+GGG - chr5_3:56584065-56584084 None:intergenic 25.0%
! TAGAAACTAACCTATAAAAG+TGG - chr5_3:56584066-56584085 None:intergenic 25.0%
! CAAATTGATGTTATAAGTCA+AGG - chr5_3:56584250-56584269 None:intergenic 25.0%
! CTTGACTTATAACATCAATT+TGG + chr5_3:56584248-56584267 Msa0819790:intron 25.0%
! AATGCAAACCAATGTTAAAT+GGG - chr5_3:56584576-56584595 None:intergenic 25.0%
! AAATGCAAACCAATGTTAAA+TGG - chr5_3:56584577-56584596 None:intergenic 25.0%
!! CCCTTTTTATAAGACAATTT+TGG + chr5_3:56584751-56584770 Msa0819790:intron 25.0%
! ATAAACTAAGTCTCTCTTAA+AGG + chr5_3:56584866-56584885 Msa0819790:intron 25.0%
! ACCAAAAGAACCATATATTT+AGG + chr5_3:56584963-56584982 Msa0819790:intron 25.0%
!! TCCTAAATATATGGTTCTTT+TGG - chr5_3:56584967-56584986 None:intergenic 25.0%
! AAGAACCATATATTTAGGAA+AGG + chr5_3:56584968-56584987 Msa0819790:intron 25.0%
! TACATCCTTTCCTAAATATA+TGG - chr5_3:56584976-56584995 None:intergenic 25.0%
!! CAAGATACAATTTTCTCTTA+TGG + chr5_3:56585057-56585076 Msa0819790:intron 25.0%
! GGAAGATAATAAGAGAATTT+GGG - chr5_3:56585541-56585560 None:intergenic 25.0%
! TGGAAGATAATAAGAGAATT+TGG - chr5_3:56585542-56585561 None:intergenic 25.0%
!!! CTACAGAAATACTTTTAAGA+TGG - chr5_3:56585702-56585721 None:intergenic 25.0%
!!! CATCTTAAAAGTATTTCTGT+AGG + chr5_3:56585700-56585719 Msa0819790:CDS 25.0%
!!! AAGGTAATGATTATTGATTC+AGG + chr5_3:56585775-56585794 Msa0819790:CDS 25.0%
! AGAAAAAGAATATGGGAAAA+AGG + chr5_3:56585852-56585871 Msa0819790:CDS 25.0%
! GAAAAAGAATATGGGAAAAA+GGG + chr5_3:56585853-56585872 Msa0819790:CDS 25.0%
! AAAATTGTTTCGAGAAAGAT+GGG + chr5_3:56585899-56585918 Msa0819790:CDS 25.0%
! TAAGCCAATTGAAGAATTAT+CGG + chr5_3:56586218-56586237 Msa0819790:CDS 25.0%
! ACACATATTCTTATTCTGAA+AGG - chr5_3:56586245-56586264 None:intergenic 25.0%
! TGTCAAGAATAATAAATGTG+TGG + chr5_3:56586319-56586338 Msa0819790:three_prime_UTR 25.0%
!! GTATGTAGTTTTCAATAAGT+TGG + chr5_3:56586348-56586367 Msa0819790:three_prime_UTR 25.0%
!! ATTTGGAGTTAATAATACCA+TGG + chr5_3:56586548-56586567 Msa0819790:three_prime_UTR 25.0%
!! TTTGGAGTTAATAATACCAT+GGG + chr5_3:56586549-56586568 Msa0819790:three_prime_UTR 25.0%
! TTCAAACAAGAAGTCAAAAA+TGG + chr5_3:56586641-56586660 Msa0819790:three_prime_UTR 25.0%
ATAAACATAATCTGAACGCA+GGG - chr5_3:56582158-56582177 None:intergenic 30.0%
! GTTAGCAGATTTTATTAGGT+TGG - chr5_3:56582394-56582413 None:intergenic 30.0%
!! GTTCTTTTGTTTGGACTTAA+AGG - chr5_3:56582448-56582467 None:intergenic 30.0%
AAGTCATCCGTTATACTTTA+AGG - chr5_3:56582560-56582579 None:intergenic 30.0%
!! TTTGATTTTTCTTGGTTGTG+AGG - chr5_3:56582605-56582624 None:intergenic 30.0%
TTAAGGCAAGATTACATTAG+GGG + chr5_3:56582906-56582925 Msa0819790:intron 30.0%
AGAAGATGATGAAGATTCAA+AGG - chr5_3:56583146-56583165 None:intergenic 30.0%
!!! TTTTGGGGTTATTTTTATGC+TGG + chr5_3:56583383-56583402 Msa0819790:intron 30.0%
!!! TTTGGGGTTATTTTTATGCT+GGG + chr5_3:56583384-56583403 Msa0819790:intron 30.0%
TTCATCATCTTCATTGAATC+AGG - chr5_3:56583592-56583611 None:intergenic 30.0%
GAAGATTCAAAGAAAGAAGA+TGG + chr5_3:56583609-56583628 Msa0819790:intron 30.0%
GAACATCTTCATCATATTTC+TGG + chr5_3:56583639-56583658 Msa0819790:intron 30.0%
! TTTGATTGGTTGATATCATG+AGG + chr5_3:56583997-56584016 Msa0819790:intron 30.0%
! TTGATTGGTTGATATCATGA+GGG + chr5_3:56583998-56584017 Msa0819790:intron 30.0%
TCTACGTGGAATGATTTAAT+CGG + chr5_3:56584103-56584122 Msa0819790:intron 30.0%
! ATAAGTCAAGGTCTTATAAC+TGG - chr5_3:56584238-56584257 None:intergenic 30.0%
AAAATACTAACGGTTGAAGT+TGG + chr5_3:56584441-56584460 Msa0819790:intron 30.0%
! CATACATTGCTCAAATTTTC+AGG + chr5_3:56584483-56584502 Msa0819790:intron 30.0%
AAAACTAACTCAAAGGATGA+CGG - chr5_3:56584537-56584556 None:intergenic 30.0%
!!! TCATCCTTTGAGTTAGTTTT+TGG + chr5_3:56584537-56584556 Msa0819790:intron 30.0%
CATACCAAAAACTAACTCAA+AGG - chr5_3:56584544-56584563 None:intergenic 30.0%
! GACACTTGTTAGTGTTAAAT+GGG - chr5_3:56584622-56584641 None:intergenic 30.0%
! TGACACTTGTTAGTGTTAAA+TGG - chr5_3:56584623-56584642 None:intergenic 30.0%
! TAACACTAACAAGTGTCATT+AGG + chr5_3:56584625-56584644 Msa0819790:intron 30.0%
! AACACTAACAAGTGTCATTA+GGG + chr5_3:56584626-56584645 Msa0819790:intron 30.0%
TGTGATGATTTGAAGGATAT+CGG + chr5_3:56585241-56585260 Msa0819790:CDS 30.0%
!!! TCTTTATCTTTTCTAGAACC+TGG - chr5_3:56585283-56585302 None:intergenic 30.0%
GTAGACTGTACTAAATCAAA+AGG + chr5_3:56585328-56585347 Msa0819790:CDS 30.0%
!!! ATCATCTTTCTTTTTTGTGC+TGG - chr5_3:56585440-56585459 None:intergenic 30.0%
CAAAATTGTTTCGAGAAAGA+TGG + chr5_3:56585898-56585917 Msa0819790:CDS 30.0%
TATATATGCTTAACAGTGCA+AGG + chr5_3:56586042-56586061 Msa0819790:CDS 30.0%
ACCGAATATACTTGGAATTA+GGG + chr5_3:56586125-56586144 Msa0819790:CDS 30.0%
GTGGCTTTCAATCTTAAAAA+AGG + chr5_3:56586165-56586184 Msa0819790:CDS 30.0%
TTGATTGTGTTCTTGAGCAT+AGG + chr5_3:56581946-56581965 Msa0819790:CDS 35.0%
ACATTCTAGTGAAATCTCCT+TGG + chr5_3:56582041-56582060 Msa0819790:CDS 35.0%
TAAACATAATCTGAACGCAG+GGG - chr5_3:56582157-56582176 None:intergenic 35.0%
GATAAACATAATCTGAACGC+AGG - chr5_3:56582159-56582178 None:intergenic 35.0%
AGGTGAGAAAAACACAAGAA+GGG - chr5_3:56582268-56582287 None:intergenic 35.0%
AAGGTGAGAAAAACACAAGA+AGG - chr5_3:56582269-56582288 None:intergenic 35.0%
TTGGAACGACAAAATTGTGA+AGG - chr5_3:56582288-56582307 None:intergenic 35.0%
! AATTTGAAACTGCAGCAGTT+TGG - chr5_3:56582307-56582326 None:intergenic 35.0%
! GCGTGTTAGCAGATTTTATT+AGG - chr5_3:56582398-56582417 None:intergenic 35.0%
TTGTTTGGACTTAAAGGCTT+TGG - chr5_3:56582442-56582461 None:intergenic 35.0%
! GAGGCTTTTGTTCTTTTGTT+TGG - chr5_3:56582457-56582476 None:intergenic 35.0%
!!! TTTTTTGGTTGCTGCTGAAA+TGG - chr5_3:56582489-56582508 None:intergenic 35.0%
!!! GGAGTTTGCTAGCATTTTTT+TGG - chr5_3:56582504-56582523 None:intergenic 35.0%
AAACTCCCAAAACATCACAA+TGG + chr5_3:56582517-56582536 Msa0819790:intron 35.0%
! TGTTGCCATTGTGATGTTTT+GGG - chr5_3:56582525-56582544 None:intergenic 35.0%
! ATACTTTAAGGATTGCTAGC+AGG - chr5_3:56582548-56582567 None:intergenic 35.0%
AGAGAGATAAAGGACCTAAA+CGG - chr5_3:56582984-56583003 None:intergenic 35.0%
ATTTCACAAATGTTGCAAGG+CGG + chr5_3:56583265-56583284 Msa0819790:intron 35.0%
! TGATTGGTTGATATCATGAG+GGG + chr5_3:56583999-56584018 Msa0819790:intron 35.0%
AAATACCCTCTAAACAAGAG+AGG + chr5_3:56584024-56584043 Msa0819790:intron 35.0%
AATACCCTCTAAACAAGAGA+GGG + chr5_3:56584025-56584044 Msa0819790:intron 35.0%
! ATTACCCTCTCTTGTTTAGA+GGG - chr5_3:56584032-56584051 None:intergenic 35.0%
ACCAACTTTCGTAATTCATG+TGG + chr5_3:56584936-56584955 Msa0819790:intron 35.0%
ACCACATGAATTACGAAAGT+TGG - chr5_3:56584940-56584959 None:intergenic 35.0%
TGTGTTAATTGTAAGTGCAC+AGG - chr5_3:56585018-56585037 None:intergenic 35.0%
TCCAGAATGTGATGATTTGA+AGG + chr5_3:56585234-56585253 Msa0819790:CDS 35.0%
TCCTTCAAATCATCACATTC+TGG - chr5_3:56585238-56585257 None:intergenic 35.0%
TTTATGGTCCAGCTAATCTT+TGG + chr5_3:56585353-56585372 Msa0819790:CDS 35.0%
GTTGTAAACCAAAGATTAGC+TGG - chr5_3:56585364-56585383 None:intergenic 35.0%
!!! TCTTTCTTTTTTGTGCTGGT+TGG - chr5_3:56585436-56585455 None:intergenic 35.0%
GATGAAGAACCAAAAAACCA+AGG + chr5_3:56585457-56585476 Msa0819790:CDS 35.0%
CTATAAGCAAGTTCACCTTA+GGG + chr5_3:56585596-56585615 Msa0819790:CDS 35.0%
GAATGACCTTGTTCTGTTAA+AGG - chr5_3:56585655-56585674 None:intergenic 35.0%
TCACGAGTGTCATCATTTAT+CGG - chr5_3:56585748-56585767 None:intergenic 35.0%
TTCTTATAAAGAACCCAACG+AGG + chr5_3:56586008-56586027 Msa0819790:CDS 35.0%
! TAATGTTTTTCCTCCTCGTT+GGG - chr5_3:56586024-56586043 None:intergenic 35.0%
! ATAATGTTTTTCCTCCTCGT+TGG - chr5_3:56586025-56586044 None:intergenic 35.0%
GAGAAAAGACCGAATATACT+TGG + chr5_3:56586117-56586136 Msa0819790:CDS 35.0%
GACCGAATATACTTGGAATT+AGG + chr5_3:56586124-56586143 Msa0819790:CDS 35.0%
GCCCTAATTCCAAGTATATT+CGG - chr5_3:56586129-56586148 None:intergenic 35.0%
AGGACCGATAATTCTTCAAT+TGG - chr5_3:56586225-56586244 None:intergenic 35.0%
TGTATATATGCCATGCCATT+TGG + chr5_3:56586264-56586283 Msa0819790:three_prime_UTR 35.0%
! TATAGCAGCCTTATACCAAA+TGG - chr5_3:56586282-56586301 None:intergenic 35.0%
! AAATCTCCTTGGAAGTGCAA+CGG + chr5_3:56582052-56582071 Msa0819790:CDS 40.0%
!! TATTGGTTCCTTCCACAATC+TGG - chr5_3:56582092-56582111 None:intergenic 40.0%
! GACCAGTGTGTTCAACTTTT+AGG + chr5_3:56582207-56582226 Msa0819790:CDS 40.0%
CTCCTAAAAGTTGAACACAC+TGG - chr5_3:56582212-56582231 None:intergenic 40.0%
TGAGAAAAACACAAGAAGGG+GGG - chr5_3:56582265-56582284 None:intergenic 40.0%
GTGAGAAAAACACAAGAAGG+GGG - chr5_3:56582266-56582285 None:intergenic 40.0%
GGTGAGAAAAACACAAGAAG+GGG - chr5_3:56582267-56582286 None:intergenic 40.0%
AAAAATCACGATACCTGACG+CGG - chr5_3:56582358-56582377 None:intergenic 40.0%
GCTGAAATGGTTATGTCATG+AGG - chr5_3:56582476-56582495 None:intergenic 40.0%
! GTGTTGCCATTGTGATGTTT+TGG - chr5_3:56582526-56582545 None:intergenic 40.0%
!! TGACGTGGTTCTGGAAATAA+CGG + chr5_3:56582653-56582672 Msa0819790:intron 40.0%
ACGGTGATTCTCACGATATT+TGG + chr5_3:56582672-56582691 Msa0819790:intron 40.0%
GAGAGATAAAGGACCTAAAC+GGG - chr5_3:56582983-56583002 None:intergenic 40.0%
! TGCAAAGCTGCTCTCAATTT+TGG + chr5_3:56583366-56583385 Msa0819790:intron 40.0%
! GCAAAGCTGCTCTCAATTTT+GGG + chr5_3:56583367-56583386 Msa0819790:intron 40.0%
! CAAAGCTGCTCTCAATTTTG+GGG + chr5_3:56583368-56583387 Msa0819790:intron 40.0%
GAGAGATAAAAGACCCGAAA+CGG + chr5_3:56583758-56583777 Msa0819790:intron 40.0%
! GATTGGTTGATATCATGAGG+GGG + chr5_3:56584000-56584019 Msa0819790:intron 40.0%
! GATTACCCTCTCTTGTTTAG+AGG - chr5_3:56584033-56584052 None:intergenic 40.0%
GATCGAGACCCATTTAACAT+TGG + chr5_3:56584565-56584584 Msa0819790:intron 40.0%
CCTAAGAAGAGTTTCTCTTG+CGG + chr5_3:56585208-56585227 Msa0819790:CDS 40.0%
!! TTTCTAGAACCTGGACATTC+TGG - chr5_3:56585274-56585293 None:intergenic 40.0%
!!! CCAACAACTCCTTGGTTTTT+TGG - chr5_3:56585469-56585488 None:intergenic 40.0%
CCAAAAAACCAAGGAGTTGT+TGG + chr5_3:56585466-56585485 Msa0819790:CDS 40.0%
GCTATAAGCAAGTTCACCTT+AGG + chr5_3:56585595-56585614 Msa0819790:CDS 40.0%
AGCACTCCTTTAACAGAACA+AGG + chr5_3:56585646-56585665 Msa0819790:CDS 40.0%
GGGAAAAAGGGAGAAAAGAA+AGG + chr5_3:56585865-56585884 Msa0819790:CDS 40.0%
TTATAAAGAACCCAACGAGG+AGG + chr5_3:56586011-56586030 Msa0819790:CDS 40.0%
ATCAAAGGCTGCGTAACTAT+GGG + chr5_3:56586082-56586101 Msa0819790:CDS 40.0%
! ATGCCATGCCATTTGGTATA+AGG + chr5_3:56586271-56586290 Msa0819790:three_prime_UTR 40.0%
TTCTTGACACACATGACTAG+AGG - chr5_3:56586308-56586327 None:intergenic 40.0%
TCAGTTGTTAGAATGACCCA+TGG - chr5_3:56586568-56586587 None:intergenic 40.0%
TGCAAAGTGTGCTTGAGCAA+AGG + chr5_3:56581977-56581996 Msa0819790:CDS 45.0%
GCAAAGTGTGCTTGAGCAAA+GGG + chr5_3:56581978-56581997 Msa0819790:CDS 45.0%
! AATCTCCTTGGAAGTGCAAC+GGG + chr5_3:56582053-56582072 Msa0819790:CDS 45.0%
ACTGGACTACTTCCAGATTG+TGG + chr5_3:56582077-56582096 Msa0819790:CDS 45.0%
GACTACTTCCAGATTGTGGA+AGG + chr5_3:56582081-56582100 Msa0819790:CDS 45.0%
! AAAGGCTTTGGCTGATAGTG+AGG - chr5_3:56582430-56582449 None:intergenic 45.0%
! GTTATTTCCAGAACCACGTC+AGG - chr5_3:56582654-56582673 None:intergenic 45.0%
CCGCAAGAGAAACTCTTCTT+AGG - chr5_3:56585211-56585230 None:intergenic 45.0%
AACCAAGGAGTTGTTGGACT+TGG + chr5_3:56585472-56585491 Msa0819790:CDS 45.0%
ATCCAAGTCCAACAACTCCT+TGG - chr5_3:56585477-56585496 None:intergenic 45.0%
GAGTTGTTGGACTTGGATCT+GGG + chr5_3:56585479-56585498 Msa0819790:CDS 45.0%
TGACACTCGTGATGCTCATA+AGG + chr5_3:56585756-56585775 Msa0819790:CDS 45.0%
CATCAAAGGCTGCGTAACTA+TGG + chr5_3:56586081-56586100 Msa0819790:CDS 45.0%
TAACTATGGGAAGTCAGAGG+AGG + chr5_3:56586095-56586114 Msa0819790:CDS 45.0%
ACTTGGAATTAGGGCACAGA+TGG + chr5_3:56586134-56586153 Msa0819790:CDS 45.0%
! TAGGGCACAGATGGATTTCT+TGG + chr5_3:56586143-56586162 Msa0819790:CDS 45.0%
! CAGCCTTATACCAAATGGCA+TGG - chr5_3:56586277-56586296 None:intergenic 45.0%
TGTGAGAGAGAGAGAGAGTT+GGG + chr5_3:56586425-56586444 Msa0819790:intron 45.0%
!! TTAAAAAAAAAATAAGATAA+AGG - chr5_3:56582933-56582952 None:intergenic 5.0%
!!! TTATTTTTTTTTTAACAATT+TGG + chr5_3:56582938-56582957 Msa0819790:intron 5.0%
!! TAGAAAATAATAATAATATA+CGG + chr5_3:56583421-56583440 Msa0819790:intron 5.0%
!!! ATAAAAAAAAAATCTATTTT+AGG - chr5_3:56583924-56583943 None:intergenic 5.0%
!! ATTGAAAATTATTATAAATA+AGG - chr5_3:56584325-56584344 None:intergenic 5.0%
!!! GATTTTTTTTTTTATATTAA+AGG + chr5_3:56585115-56585134 Msa0819790:intron 5.0%
!!! TTTAAAATAAAATTTCTTTT+TGG + chr5_3:56586729-56586748 Msa0819790:three_prime_UTR 5.0%
! ATCTCCTTGGAAGTGCAACG+GGG + chr5_3:56582054-56582073 Msa0819790:CDS 50.0%
!! GTTGTTTCTAGAACCGCGTC+AGG + chr5_3:56582342-56582361 Msa0819790:CDS 50.0%
TCAACTCACCATACCTGACG+TGG + chr5_3:56582638-56582657 Msa0819790:intron 50.0%
TTCCAGAACCACGTCAGGTA+TGG - chr5_3:56582649-56582668 None:intergenic 50.0%
CACAAATGTTGCAAGGCGGA+TGG + chr5_3:56583269-56583288 Msa0819790:intron 50.0%
AAATGTTGCAAGGCGGATGG+TGG + chr5_3:56583272-56583291 Msa0819790:intron 50.0%
AGGCTTAGTCACTAGCGGAA+GGG + chr5_3:56583295-56583314 Msa0819790:intron 50.0%
TAGTCACTAGCGGAAGGGTT+AGG + chr5_3:56583300-56583319 Msa0819790:intron 50.0%
GGCATCAAACCAGAATGTCC+AGG + chr5_3:56585262-56585281 Msa0819790:CDS 50.0%
GGAGTTGTTGGACTTGGATC+TGG + chr5_3:56585478-56585497 Msa0819790:CDS 50.0%
TCGTCTTTGCGACGAGATTG+TGG - chr5_3:56585562-56585581 None:intergenic 50.0%
AGCAAGTTCACCTTAGGGCA+CGG + chr5_3:56585601-56585620 Msa0819790:CDS 50.0%
AAATTCCACACCGTGCCCTA+AGG - chr5_3:56585614-56585633 None:intergenic 50.0%
! AGGGCACGGTGTGGAATTTT+TGG + chr5_3:56585615-56585634 Msa0819790:CDS 50.0%
GCGTAACTATGGGAAGTCAG+AGG + chr5_3:56586092-56586111 Msa0819790:CDS 50.0%
! GGCACAGATGGATTTCTTGG+TGG + chr5_3:56586146-56586165 Msa0819790:CDS 50.0%
GTGTGAGAGAGAGAGAGAGT+TGG + chr5_3:56586424-56586443 Msa0819790:intron 50.0%
! GCTTTGGCTGATAGTGAGGC+AGG - chr5_3:56582426-56582445 None:intergenic 55.0%
CACCATACCTGACGTGGTTC+TGG + chr5_3:56582644-56582663 Msa0819790:intron 55.0%
GGTGGAGGCTTAGTCACTAG+CGG + chr5_3:56583290-56583309 Msa0819790:intron 55.0%
GAGGCTTAGTCACTAGCGGA+AGG + chr5_3:56583294-56583313 Msa0819790:intron 55.0%
! GAGAGAGTTGGGAGTGTGCA+TGG + chr5_3:56586436-56586455 Msa0819790:intron 55.0%
CAGTCCCCGTTGCACTTCCA+AGG - chr5_3:56582061-56582080 None:intergenic 60.0%
! CTTGGAAGTGCAACGGGGAC+TGG + chr5_3:56582059-56582078 Msa0819790:CDS 60.0%
! TGTTGCAAGGCGGATGGTGG+AGG + chr5_3:56583275-56583294 Msa0819790:intron 60.0%
GTTCACCTTAGGGCACGGTG+TGG + chr5_3:56585606-56585625 Msa0819790:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 56581910 56586808 56581910 ID=Msa0819790;Name=Msa0819790
chr5_3 mRNA 56581910 56586808 56581910 ID=Msa0819790-mRNA-1;Parent=Msa0819790;Name=Msa0819790-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0.5|0.66|0.66|0.5|0.33|3|528|523
chr5_3 exon 56581910 56582363 56581910 ID=Msa0819790-mRNA-1:exon:17076;Parent=Msa0819790-mRNA-1
chr5_3 exon 56585137 56586423 56585137 ID=Msa0819790-mRNA-1:exon:17077;Parent=Msa0819790-mRNA-1
chr5_3 exon 56586450 56586808 56586450 ID=Msa0819790-mRNA-1:exon:17078;Parent=Msa0819790-mRNA-1
chr5_3 CDS 56581910 56582363 56581910 ID=Msa0819790-mRNA-1:cds;Parent=Msa0819790-mRNA-1
chr5_3 CDS 56585137 56586254 56585137 ID=Msa0819790-mRNA-1:cds;Parent=Msa0819790-mRNA-1
chr5_3 three_prime_UTR 56586255 56586423 56586255 ID=Msa0819790-mRNA-1:three_prime_utr;Parent=Msa0819790-mRNA-1
chr5_3 three_prime_UTR 56586450 56586808 56586450 ID=Msa0819790-mRNA-1:three_prime_utr;Parent=Msa0819790-mRNA-1
Gene Sequence

>Msa0819790

ATGTCTCTTGCAGTTGTGCTTGAGCATAGAAGTCTCTTGATTGTGTTCTTGAGCATAGGAAGACTCTTGCAAAGTGTGCTTGAGCAAAGGGAGTCTCTTGCTGTGTGCTTGAGCAATTGTAATCAGTTATTACATTCTAGTGAAATCTCCTTGGAAGTGCAACGGGGACTGGACTACTTCCAGATTGTGGAAGGAACCAATATAATTGCTTTGTGTCTTTCTTCTTCTCTTTCTCTGTTTTATTCCCCTGCGTTCAGATTATGTTTATCACTTCAGAAGCTGTATCACTTAGCTTCTGACCAGTGTGTTCAACTTTTAGGAGAAACGAAGAATTTTTCAAAACACAATTCAACCCCCCTTCTTGTGTTTTTCTCACCTTCACAATTTTGTCGTTCCAAACTGCTGCAGTTTCAAATTATAGTGAGAATTGTCGTTGTTTCTAGAACCGCGTCAGGTGGAGAGAGCGAAGATAGCAAATGTTATTGTGCAAAATATGAGAAAAGTAAAGAAGCATGTATCGAGAAGCCTAAGAAGAGTTTCTCTTGCGGAAATCCAGAATGTGATGATTTGAAGGATATCGGCATCAAACCAGAATGTCCAGGTTCTAGAAAAGATAAAGATTTATGCGAGTTTAGTATGAGTTATGTAGACTGTACTAAATCAAAAGGAATTTATGGTCCAGCTAATCTTTGGTTTACAACGATCAAAAGTGAAACCAAAAAAGAAAGATTGAACATTTGGTTTTCATTTCCAACCAGCACAAAAAAGAAAGATGATGAAGAACCAAAAAACCAAGGAGTTGTTGGACTTGGATCTGGGAAGTTATCTTTGATAAAACAGTATCAACACATCACTCCCAAATTCTCTTATTATCTTCCACAATCTCGTCGCAAAGACGAAGAAGATATTAAAGCTATAAGCAAGTTCACCTTAGGGCACGGTGTGGAATTTTTGGAGAAGTTGAGCACTCCTTTAACAGAACAAGGTCATTCTTTGTGTGAAACTCGTTACTGCGTCCATCTTAAAAGTATTTCTGTAGGTGAAAGTGAAAATAACAAAATACCGATAAATGATGACACTCGTGATGCTCATAAGGTAATGATTATTGATTCAGGTCACACATTTACACATCTTAGTAAAGATATATTCGACAAATTTTTAGAAAAAGTAGAAAAAGAATATGGGAAAAAGGGAGAAAAGAAAGGCTGTGATTATCAAAATTGTTTCGAGAAAGATGGGAAAGTTGAGAAATTATTGAAGAAAATATCATTTGAGTTTCAAGATATAAAAATTGAGTTGAAGAGGGAGAACTTTTTAGATGATTTTTCTTATAAAGAACCCAACGAGGAGGAAAAACATTATATATGCTTAACAGTGCAAGGAGAAAATGAAAAGTATCATCAAAGGCTGCGTAACTATGGGAAGTCAGAGGAGGAGAAAAGACCGAATATACTTGGAATTAGGGCACAGATGGATTTCTTGGTGGCTTTCAATCTTAAAAAAGGTATTAATAAAGTGTCTTTTGAGGATAAGAGTAAGCCAATTGAAGAATTATCGGTCCTTTCAGAATAA
Protein sequence

>Msa0819790

MSLAVVLEHRSLLIVFLSIGRLLQSVLEQRESLAVCLSNCNQLLHSSEISLEVQRGLDYFQIVEGTNIIALCLSSSLSLFYSPAFRLCLSLQKLYHLASDQCVQLLGETKNFSKHNSTPLLVFFSPSQFCRSKLLQFQIIVRIVVVSRTASGGESEDSKCYCAKYEKSKEACIEKPKKSFSCGNPECDDLKDIGIKPECPGSRKDKDLCEFSMSYVDCTKSKGIYGPANLWFTTIKSETKKERLNIWFSFPTSTKKKDDEEPKNQGVVGLGSGKLSLIKQYQHITPKFSYYLPQSRRKDEEDIKAISKFTLGHGVEFLEKLSTPLTEQGHSLCETRYCVHLKSISVGESENNKIPINDDTRDAHKVMIIDSGHTFTHLSKDIFDKFLEKVEKEYGKKGEKKGCDYQNCFEKDGKVEKLLKKISFEFQDIKIELKRENFLDDFSYKEPNEEEKHYICLTVQGENEKYHQRLRNYGKSEEEKRPNILGIRAQMDFLVAFNLKKGINKVSFEDKSKPIEELSVLSE*