AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0785090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0785090 MtrunA17_Chr5g0436371 93.333 150 9 1 2 150 19 168 4.26e-91 266
Msa0785090 MtrunA17_Chr7g0258381 91.406 128 11 0 2 129 19 146 2.92e-77 226
Msa0785090 MtrunA17_Chr3g0113091 59.155 142 58 0 10 151 30 171 6.21e-58 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0785090 AT2G39920.4 44.118 136 75 1 2 136 21 156 3.90e-38 131
Msa0785090 AT2G39920.3 44.118 136 75 1 2 136 21 156 3.90e-38 131
Msa0785090 AT2G39920.1 44.118 136 75 1 2 136 21 156 3.90e-38 131
Msa0785090 AT2G39920.2 44.118 136 75 1 2 136 21 156 3.98e-38 131

Find 77 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGCTAAGCTCAATAATT+TGG 0.240869 5_2:+69898113 Msa0785090:CDS
TTTAACATTATCTAGAATTA+AGG 0.247976 5_2:-69898716 None:intergenic
TGTTTGTATATAAACTTAAA+TGG 0.251921 5_2:+69900013 Msa0785090:three_prime_UTR
TGCCGCAGTTCTAAGGGTTT+TGG 0.255038 5_2:+69899872 Msa0785090:three_prime_UTR
AAGGAATGCGATACAGAAAA+AGG 0.274169 5_2:+69899535 Msa0785090:three_prime_UTR
GTTGATTGATGGTGACATTT+TGG 0.278987 5_2:-69899081 None:intergenic
ATAGGTGCTGGATATATATT+AGG 0.288215 5_2:+69899685 Msa0785090:three_prime_UTR
AATGAGAATTCCATTGAAAA+AGG 0.302478 5_2:-69898302 None:intergenic
AAATTACCTTAGTAACAAAT+TGG 0.311682 5_2:-69898328 None:intergenic
GGTGTTCTGCCGCAGTTCTA+AGG 0.316307 5_2:+69899865 Msa0785090:three_prime_UTR
TAAGTTTATATACAAACATT+AGG 0.318115 5_2:-69900008 None:intergenic
CTTGTTGATGCTGGATTTAG+AGG 0.329191 5_2:+69899103 Msa0785090:three_prime_UTR
ATTGCTGGATTTGCAGCTCT+TGG 0.339380 5_2:+69897964 Msa0785090:CDS
GTAATCCTCAATCACAGATT+TGG 0.344974 5_2:-69898220 None:intergenic
AAATTTACAAATTATGTGTT+TGG 0.346031 5_2:-69899903 None:intergenic
CTTAGAAAATCACCGTAAAA+AGG 0.359497 5_2:-69899814 None:intergenic
AGTCACTTTGTTGGTTTCAA+TGG 0.364410 5_2:+69897996 Msa0785090:CDS
ATTCTTCCAATTTGTTACTA+AGG 0.365366 5_2:+69898322 Msa0785090:CDS
TCAGCAACAATCTTCATTGC+TGG 0.372517 5_2:+69897949 Msa0785090:CDS
ATTCTTGACTGCAAGTAATT+CGG 0.392444 5_2:+69898524 Msa0785090:three_prime_UTR
TGCATATGTGAATATTGAAA+TGG 0.408166 5_2:+69900053 Msa0785090:three_prime_UTR
GGCTCAAACATGTCACATAT+AGG 0.408550 5_2:-69899650 None:intergenic
ACAGATAAATGCTAAGATCT+AGG 0.412568 5_2:+69898740 Msa0785090:three_prime_UTR
TCTAGAATTAAGGTACATAA+TGG 0.416854 5_2:-69898706 None:intergenic
ATCAATCAACTTGTTGATGC+TGG 0.420104 5_2:+69899094 Msa0785090:three_prime_UTR
AGCATCAACAAGTTGATTGA+TGG 0.423312 5_2:-69899092 None:intergenic
TAGACCAACCACTAGCTTGA+AGG 0.432274 5_2:-69899027 None:intergenic
TAATGTTCCTAACATTTGTA+AGG 0.446915 5_2:+69898143 Msa0785090:CDS
AAAGGTGTGTTTGCGTATGT+TGG 0.447162 5_2:+69899755 Msa0785090:three_prime_UTR
GTGTAGCTTCCACGTCAATC+AGG 0.447983 5_2:+69899844 Msa0785090:three_prime_UTR
TCATGTCAAAATAGCAATGC+TGG 0.451480 5_2:+69898033 Msa0785090:CDS
TTGATGCTGGATTTAGAGGC+TGG 0.460574 5_2:+69899107 Msa0785090:three_prime_UTR
TAAATATGAATCACAACTGC+GGG 0.462864 5_2:+69900162 Msa0785090:three_prime_UTR
GCTCAAACATGTCACATATA+GGG 0.465466 5_2:-69899649 None:intergenic
ACTGCAAGTAATTCGGATCC+TGG 0.474398 5_2:+69898531 Msa0785090:three_prime_UTR
TTCAACCAAATCTGTGATTG+AGG 0.476332 5_2:+69898215 Msa0785090:CDS
GCCAGGTCCTTACAAATGTT+AGG 0.483970 5_2:-69898150 None:intergenic
GGATTTGGATTTCATTTAGT+CGG 0.491559 5_2:+69898395 Msa0785090:three_prime_UTR
AACATGTCACATATAGGGTC+TGG 0.494617 5_2:-69899644 None:intergenic
GGTGTTAAACCATCAGAAGA+TGG 0.502777 5_2:+69898249 Msa0785090:CDS
GTGTTCTGCCGCAGTTCTAA+GGG 0.505357 5_2:+69899866 Msa0785090:three_prime_UTR
TCCTAACATTTGTAAGGACC+TGG 0.516716 5_2:+69898149 Msa0785090:CDS
TATGTGACATGTTTGAGCCT+TGG 0.521587 5_2:+69899654 Msa0785090:three_prime_UTR
ATTAGTAAACTAATGCAAGT+TGG 0.523545 5_2:-69898799 None:intergenic
ACTGCGGGGTCAATTTCGTC+AGG 0.527374 5_2:+69900177 Msa0785090:three_prime_UTR
ACTGTTACAGATGCAGATAC+CGG 0.529547 5_2:+69899602 Msa0785090:three_prime_UTR
AAGGTGTGTTTGCGTATGTT+GGG 0.529898 5_2:+69899756 Msa0785090:three_prime_UTR
CTTTGATATATTGTAAAGCC+AGG 0.532125 5_2:-69898167 None:intergenic
TTAAGGTACATAATGGATAG+AGG 0.533805 5_2:-69898699 None:intergenic
CTGATGGTTTAACACCATTG+AGG 0.534727 5_2:-69898242 None:intergenic
GTGACATGTTTGAGCCTTGG+AGG 0.550861 5_2:+69899657 Msa0785090:three_prime_UTR
ATGTCACTGTCCCATGGCGG+TGG 0.553409 5_2:-69899790 None:intergenic
TTGGAGGAGAGCATAGGTGC+TGG 0.554025 5_2:+69899673 Msa0785090:three_prime_UTR
TTTACTAATATTGCAAAACC+AGG 0.576428 5_2:-69898549 None:intergenic
TGGTCAATATGCAAGAGACT+TGG 0.581585 5_2:+69898191 Msa0785090:CDS
TACAAGAACCTTCAAGCTAG+TGG 0.590030 5_2:+69899019 Msa0785090:three_prime_UTR
TGAGCCTTGGAGGAGAGCAT+AGG 0.590869 5_2:+69899667 Msa0785090:three_prime_UTR
GTGATTGAGGATTACCTCAA+TGG 0.590987 5_2:+69898228 Msa0785090:CDS
AGCACCTATGCTCTCCTCCA+AGG 0.591345 5_2:-69899671 None:intergenic
GCTGGTCTTCATTGCTGATG+AGG 0.591699 5_2:+69899125 Msa0785090:three_prime_UTR
ATGCTAAGATCTAGGACAGG+TGG 0.596220 5_2:+69898748 Msa0785090:three_prime_UTR
GCATCAAAACCATCTTCTGA+TGG 0.598401 5_2:-69898258 None:intergenic
GTACAATAATTACATTTGAG+CGG 0.599482 5_2:-69898839 None:intergenic
ATGAAATTACATCTTCAAAG+TGG 0.602191 5_2:-69898614 None:intergenic
TTGTTAGCAGTAACTTGACA+AGG 0.606385 5_2:+69898905 Msa0785090:three_prime_UTR
CTGGCTTTACAATATATCAA+AGG 0.614149 5_2:+69898168 Msa0785090:CDS
AAAGGTATGTCACTGTCCCA+TGG 0.614415 5_2:-69899796 None:intergenic
TAAATGCTAAGATCTAGGAC+AGG 0.619653 5_2:+69898745 Msa0785090:three_prime_UTR
CTAAATATGAATCACAACTG+CGG 0.621433 5_2:+69900161 Msa0785090:three_prime_UTR
AGAACCTTCAAGCTAGTGGT+TGG 0.622868 5_2:+69899023 Msa0785090:three_prime_UTR
CTTGCAGTCAAGAATCTACA+CGG 0.626118 5_2:-69898516 None:intergenic
GCTTTACAATATATCAAAGG+TGG 0.628623 5_2:+69898171 Msa0785090:CDS
CGGAAAACTTAAAGTCGAGA+CGG 0.644006 5_2:+69898415 Msa0785090:three_prime_UTR
GTCCAAAACCCTTAGAACTG+CGG 0.710356 5_2:-69899874 None:intergenic
GGCAGAACACCTGATTGACG+TGG 0.718981 5_2:-69899853 None:intergenic
AAATATGAATCACAACTGCG+GGG 0.744274 5_2:+69900163 Msa0785090:three_prime_UTR
GGTATGTCACTGTCCCATGG+CGG 0.745152 5_2:-69899793 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATCATTTAAGATT+TGG + chr5_2:69898345-69898364 Msa0785090:exon 10.0%
!!! ATTTTTATTAGTTAAGTTTT+TGG + chr5_2:69898373-69898392 Msa0785090:three_prime_UTR 10.0%
!!! TTTTTATTAGTTAAGTTTTT+GGG + chr5_2:69898374-69898393 Msa0785090:three_prime_UTR 10.0%
!!! TATTTTTTGTATATTAAGAA+AGG + chr5_2:69899737-69899756 Msa0785090:three_prime_UTR 10.0%
!! ATATAACAAACAACATTATT+TGG + chr5_2:69897227-69897246 Msa0785090:intron 15.0%
!! TATAACAAACAACATTATTT+GGG + chr5_2:69897228-69897247 Msa0785090:intron 15.0%
!!! CTAATTTTGATTAGTATTTT+TGG + chr5_2:69897853-69897872 Msa0785090:intron 15.0%
!!! TTTAACATTATCTAGAATTA+AGG - chr5_2:69898719-69898738 None:intergenic 15.0%
!! AAATTTACAAATTATGTGTT+TGG - chr5_2:69899906-69899925 None:intergenic 15.0%
!! TAAGTTTATATACAAACATT+AGG - chr5_2:69900011-69900030 None:intergenic 15.0%
!! TGTTTGTATATAAACTTAAA+TGG + chr5_2:69900013-69900032 Msa0785090:three_prime_UTR 15.0%
!! TAATTGCACTAAACAAATTT+AGG - chr5_2:69897278-69897297 None:intergenic 20.0%
!!! TATTTTTTCAATGAAACTGA+AGG + chr5_2:69897337-69897356 Msa0785090:intron 20.0%
!! AACAATTATTATCACCTATA+TGG + chr5_2:69897544-69897563 Msa0785090:intron 20.0%
!!! TTTTAGTCTTTGAAATTGTA+GGG + chr5_2:69897594-69897613 Msa0785090:intron 20.0%
!!! TTTTTTTTAGATCAGTTAGT+CGG - chr5_2:69897698-69897717 None:intergenic 20.0%
!!! TTCATATTCAAAAGTCATTT+TGG + chr5_2:69897738-69897757 Msa0785090:intron 20.0%
!! ATTTAGTCCTTAAAACTATA+GGG - chr5_2:69897771-69897790 None:intergenic 20.0%
!! AATTTAGTCCTTAAAACTAT+AGG - chr5_2:69897772-69897791 None:intergenic 20.0%
!! AAATTACCTTAGTAACAAAT+TGG - chr5_2:69898331-69898350 None:intergenic 20.0%
!! TATCTATAAAACAACATTTG+TGG - chr5_2:69899195-69899214 None:intergenic 20.0%
!!! TGTTGTTTTATAGATATCTA+AGG + chr5_2:69899199-69899218 Msa0785090:intron 20.0%
!! AGAAAAATATACATTTGCTT+TGG - chr5_2:69899510-69899529 None:intergenic 20.0%
!!! ATGTATATTTTTCTAGACAA+AGG + chr5_2:69899516-69899535 Msa0785090:three_prime_UTR 20.0%
! GAATTATGGATGAAAGATTA+TGG - chr5_2:69897060-69897079 None:intergenic 25.0%
! ATAATAATAATGTCTGTCCT+AGG + chr5_2:69897107-69897126 Msa0785090:intron 25.0%
! ATGTTGTGTTAGAATCTTAT+TGG + chr5_2:69897199-69897218 Msa0785090:intron 25.0%
!!! GTTTTAGGTGTTATTTGAAT+TGG + chr5_2:69897421-69897440 Msa0785090:intron 25.0%
!!! GTTTTAGTCTTTGAAATTGT+AGG + chr5_2:69897593-69897612 Msa0785090:intron 25.0%
!!! TTTAGTCTTTGAAATTGTAG+GGG + chr5_2:69897595-69897614 Msa0785090:intron 25.0%
!!! AATTTCATTGATTTAGACGA+TGG - chr5_2:69897631-69897650 None:intergenic 25.0%
!!! ACTTTTATTAGTCACTTTGT+TGG + chr5_2:69897987-69898006 Msa0785090:CDS 25.0%
! TAATGTTCCTAACATTTGTA+AGG + chr5_2:69898143-69898162 Msa0785090:CDS 25.0%
! AATGAGAATTCCATTGAAAA+AGG - chr5_2:69898305-69898324 None:intergenic 25.0%
!!! ATTCTTCCAATTTGTTACTA+AGG + chr5_2:69898322-69898341 Msa0785090:CDS 25.0%
!!! TTAGTTAAGTTTTTGGGATT+TGG + chr5_2:69898380-69898399 Msa0785090:three_prime_UTR 25.0%
! TTTACTAATATTGCAAAACC+AGG - chr5_2:69898552-69898571 None:intergenic 25.0%
! ATGAAATTACATCTTCAAAG+TGG - chr5_2:69898617-69898636 None:intergenic 25.0%
!! TCTAGAATTAAGGTACATAA+TGG - chr5_2:69898709-69898728 None:intergenic 25.0%
! ATTAGTAAACTAATGCAAGT+TGG - chr5_2:69898802-69898821 None:intergenic 25.0%
! GTACAATAATTACATTTGAG+CGG - chr5_2:69898842-69898861 None:intergenic 25.0%
!! AACCATTATCTAGGATATAT+GGG - chr5_2:69899163-69899182 None:intergenic 25.0%
! TCTGCAAAAATAGAACATAT+TGG - chr5_2:69899468-69899487 None:intergenic 25.0%
! TGCATATGTGAATATTGAAA+TGG + chr5_2:69900053-69900072 Msa0785090:three_prime_UTR 25.0%
GACATAGACAAAGTGAATTA+TGG - chr5_2:69897074-69897093 None:intergenic 30.0%
TTGCACTAAACAAATTTAGG+TGG - chr5_2:69897275-69897294 None:intergenic 30.0%
TAGTGCAATTAATGTTGTCT+AGG + chr5_2:69897287-69897306 Msa0785090:intron 30.0%
!! CCTGAAATTTTAGAGAAACT+TGG - chr5_2:69897376-69897395 None:intergenic 30.0%
!! CCAAGTTTCTCTAAAATTTC+AGG + chr5_2:69897373-69897392 Msa0785090:intron 30.0%
! GATTTCTTGAAGCAAGTTTT+AGG + chr5_2:69897406-69897425 Msa0785090:intron 30.0%
CCAAAGCTTAATCTATCTTT+AGG - chr5_2:69897449-69897468 None:intergenic 30.0%
CCTAAAGATAGATTAAGCTT+TGG + chr5_2:69897446-69897465 Msa0785090:intron 30.0%
! AGTGTTATTATGTCTGCTTA+TGG + chr5_2:69897469-69897488 Msa0785090:intron 30.0%
TTGTAGAATACATATGCTCA+TGG - chr5_2:69897495-69897514 None:intergenic 30.0%
ATGAGCATATGTATTCTACA+AGG + chr5_2:69897494-69897513 Msa0785090:intron 30.0%
! TTCGATCATACTAGTCATTT+TGG + chr5_2:69897658-69897677 Msa0785090:intron 30.0%
TCTGTGAACAAGAAAAAAAC+AGG - chr5_2:69897880-69897899 None:intergenic 30.0%
!!! TTTTTTCTTGTTCACAGAGA+TGG + chr5_2:69897882-69897901 Msa0785090:intron 30.0%
! TTTTTCTTGTTCACAGAGAT+GGG + chr5_2:69897883-69897902 Msa0785090:intron 30.0%
CTTTGATATATTGTAAAGCC+AGG - chr5_2:69898170-69898189 None:intergenic 30.0%
!! CTGGCTTTACAATATATCAA+AGG + chr5_2:69898168-69898187 Msa0785090:CDS 30.0%
GCTTTACAATATATCAAAGG+TGG + chr5_2:69898171-69898190 Msa0785090:CDS 30.0%
!!! ACATAGATAGCCTTTTTCAA+TGG + chr5_2:69898292-69898311 Msa0785090:CDS 30.0%
GGATTTGGATTTCATTTAGT+CGG + chr5_2:69898395-69898414 Msa0785090:three_prime_UTR 30.0%
ATTCTTGACTGCAAGTAATT+CGG + chr5_2:69898524-69898543 Msa0785090:three_prime_UTR 30.0%
!! TTAAGGTACATAATGGATAG+AGG - chr5_2:69898702-69898721 None:intergenic 30.0%
ACAGATAAATGCTAAGATCT+AGG + chr5_2:69898740-69898759 Msa0785090:three_prime_UTR 30.0%
! AACTTGACAAGGTTTTCTTT+TGG + chr5_2:69898916-69898935 Msa0785090:three_prime_UTR 30.0%
!! ATTTTGTTATCAAGAGAGTC+AGG + chr5_2:69899052-69899071 Msa0785090:three_prime_UTR 30.0%
! CAACCATTATCTAGGATATA+TGG - chr5_2:69899164-69899183 None:intergenic 30.0%
! CATATATCCTAGATAATGGT+TGG + chr5_2:69899162-69899181 Msa0785090:intron 30.0%
! TTTTTCTCCAACCATTATCT+AGG - chr5_2:69899172-69899191 None:intergenic 30.0%
TCGATTACAAAGTACATGTT+TGG + chr5_2:69899291-69899310 Msa0785090:intron 30.0%
AAAGTACATGTTTGGATTGA+CGG + chr5_2:69899299-69899318 Msa0785090:intron 30.0%
AACATGTCACATATACTTTG+TGG - chr5_2:69899380-69899399 None:intergenic 30.0%
TCACATACTTTGTGTATGTT+TGG - chr5_2:69899428-69899447 None:intergenic 30.0%
! ACATATTGGTGCTTAAAATC+TGG - chr5_2:69899454-69899473 None:intergenic 30.0%
!! ATAGGTGCTGGATATATATT+AGG + chr5_2:69899685-69899704 Msa0785090:three_prime_UTR 30.0%
!! AGGTTTTATCCTAGAGTTTT+TGG + chr5_2:69899705-69899724 Msa0785090:three_prime_UTR 30.0%
TTTATCGAACCAAAAACTCT+AGG - chr5_2:69899717-69899736 None:intergenic 30.0%
CTTAGAAAATCACCGTAAAA+AGG - chr5_2:69899817-69899836 None:intergenic 30.0%
AACACATGCGTGAAAAAATA+TGG + chr5_2:69900111-69900130 Msa0785090:three_prime_UTR 30.0%
CTAAATATGAATCACAACTG+CGG + chr5_2:69900161-69900180 Msa0785090:three_prime_UTR 30.0%
TAAATATGAATCACAACTGC+GGG + chr5_2:69900162-69900181 Msa0785090:three_prime_UTR 30.0%
TTTAGGTGGAAGAAAAAGAG+TGG - chr5_2:69897261-69897280 None:intergenic 35.0%
TAAAATTTCAGGACACACCT+TGG + chr5_2:69897384-69897403 Msa0785090:intron 35.0%
! AACTTGCTTCAAGAAATCCA+AGG - chr5_2:69897404-69897423 None:intergenic 35.0%
ATGAGCAGAATCTACCATAT+AGG - chr5_2:69897561-69897580 None:intergenic 35.0%
! TTTTCTTGTTCACAGAGATG+GGG + chr5_2:69897884-69897903 Msa0785090:intron 35.0%
!!! AGTAGTTTTGTGCTTGAATC+AGG + chr5_2:69897907-69897926 Msa0785090:CDS 35.0%
!! AGTCACTTTGTTGGTTTCAA+TGG + chr5_2:69897996-69898015 Msa0785090:CDS 35.0%
TCATGTCAAAATAGCAATGC+TGG + chr5_2:69898033-69898052 Msa0785090:CDS 35.0%
GCATGCTAAGCTCAATAATT+TGG + chr5_2:69898113-69898132 Msa0785090:CDS 35.0%
TTCAACCAAATCTGTGATTG+AGG + chr5_2:69898215-69898234 Msa0785090:CDS 35.0%
GTAATCCTCAATCACAGATT+TGG - chr5_2:69898223-69898242 None:intergenic 35.0%
!!! ATCAGAAGATGGTTTTGATG+CGG + chr5_2:69898260-69898279 Msa0785090:CDS 35.0%
!! AACGGTCTGAATTTTGAGTA+TGG - chr5_2:69898483-69898502 None:intergenic 35.0%
! TAAATGCTAAGATCTAGGAC+AGG + chr5_2:69898745-69898764 Msa0785090:three_prime_UTR 35.0%
TTGTTAGCAGTAACTTGACA+AGG + chr5_2:69898905-69898924 Msa0785090:three_prime_UTR 35.0%
!! ACAAGGTTTTCTTTTGGTGT+TGG + chr5_2:69898922-69898941 Msa0785090:three_prime_UTR 35.0%
!!! GTTGATTGATGGTGACATTT+TGG - chr5_2:69899084-69899103 None:intergenic 35.0%
!! AGCATCAACAAGTTGATTGA+TGG - chr5_2:69899095-69899114 None:intergenic 35.0%
!! ATCAATCAACTTGTTGATGC+TGG + chr5_2:69899094-69899113 Msa0785090:three_prime_UTR 35.0%
! CATTATCTAGGATATATGGG+TGG - chr5_2:69899160-69899179 None:intergenic 35.0%
CACCCATATATCCTAGATAA+TGG + chr5_2:69899158-69899177 Msa0785090:intron 35.0%
CAATGAGTTTGGCAAAATCA+CGG - chr5_2:69899352-69899371 None:intergenic 35.0%
TTGTGGATGAACAATGAGTT+TGG - chr5_2:69899363-69899382 None:intergenic 35.0%
! TTTTGCAGAACAGAAGATGA+AGG + chr5_2:69899479-69899498 Msa0785090:intron 35.0%
AAGGAATGCGATACAGAAAA+AGG + chr5_2:69899535-69899554 Msa0785090:three_prime_UTR 35.0%
AGGAATGCGATACAGAAAAA+GGG + chr5_2:69899536-69899555 Msa0785090:three_prime_UTR 35.0%
TAGCAAAAACTTTCGCATTC+CGG - chr5_2:69899624-69899643 None:intergenic 35.0%
GCTCAAACATGTCACATATA+GGG - chr5_2:69899652-69899671 None:intergenic 35.0%
! GACAGTGACATACCTTTTTA+CGG + chr5_2:69899802-69899821 Msa0785090:three_prime_UTR 35.0%
!!! TTTTTTCACGCATGTGTTCT+TGG - chr5_2:69900109-69900128 None:intergenic 35.0%
AAATATGAATCACAACTGCG+GGG + chr5_2:69900163-69900182 Msa0785090:three_prime_UTR 35.0%
!! AGACCTGCCCTATAGTTTTA+AGG + chr5_2:69897761-69897780 Msa0785090:intron 40.0%
AGTCCTTAAAACTATAGGGC+AGG - chr5_2:69897767-69897786 None:intergenic 40.0%
TCAGCAACAATCTTCATTGC+TGG + chr5_2:69897949-69897968 Msa0785090:CDS 40.0%
TCCTAACATTTGTAAGGACC+TGG + chr5_2:69898149-69898168 Msa0785090:CDS 40.0%
TGGTCAATATGCAAGAGACT+TGG + chr5_2:69898191-69898210 Msa0785090:CDS 40.0%
GTGATTGAGGATTACCTCAA+TGG + chr5_2:69898228-69898247 Msa0785090:CDS 40.0%
CTGATGGTTTAACACCATTG+AGG - chr5_2:69898245-69898264 None:intergenic 40.0%
GGTGTTAAACCATCAGAAGA+TGG + chr5_2:69898249-69898268 Msa0785090:CDS 40.0%
GCATCAAAACCATCTTCTGA+TGG - chr5_2:69898261-69898280 None:intergenic 40.0%
CGGAAAACTTAAAGTCGAGA+CGG + chr5_2:69898415-69898434 Msa0785090:three_prime_UTR 40.0%
CACGGTTAGAAAAAGACGAA+CGG - chr5_2:69898501-69898520 None:intergenic 40.0%
CTTGCAGTCAAGAATCTACA+CGG - chr5_2:69898519-69898538 None:intergenic 40.0%
TACAAGAACCTTCAAGCTAG+TGG + chr5_2:69899019-69899038 Msa0785090:three_prime_UTR 40.0%
CTTGTTGATGCTGGATTTAG+AGG + chr5_2:69899103-69899122 Msa0785090:three_prime_UTR 40.0%
CGATACAGAAAAAGGGTTTC+AGG + chr5_2:69899543-69899562 Msa0785090:three_prime_UTR 40.0%
ACTGTTACAGATGCAGATAC+CGG + chr5_2:69899602-69899621 Msa0785090:three_prime_UTR 40.0%
AACATGTCACATATAGGGTC+TGG - chr5_2:69899647-69899666 None:intergenic 40.0%
GGCTCAAACATGTCACATAT+AGG - chr5_2:69899653-69899672 None:intergenic 40.0%
TATGTGACATGTTTGAGCCT+TGG + chr5_2:69899654-69899673 Msa0785090:three_prime_UTR 40.0%
! AAAGGTGTGTTTGCGTATGT+TGG + chr5_2:69899755-69899774 Msa0785090:three_prime_UTR 40.0%
AAGGTGTGTTTGCGTATGTT+GGG + chr5_2:69899756-69899775 Msa0785090:three_prime_UTR 40.0%
TGCAGTTAAGCCGGTGATTT+GGG - chr5_2:69897150-69897169 None:intergenic 45.0%
GAACATGTGTGCAGTTAAGC+CGG - chr5_2:69897159-69897178 None:intergenic 45.0%
CTGCACACATGTTCACAACA+TGG + chr5_2:69897165-69897184 Msa0785090:intron 45.0%
!! CTTTGAAATTGTAGGGGACG+AGG + chr5_2:69897601-69897620 Msa0785090:intron 45.0%
ATTGCTGGATTTGCAGCTCT+TGG + chr5_2:69897964-69897983 Msa0785090:CDS 45.0%
GCCAGGTCCTTACAAATGTT+AGG - chr5_2:69898153-69898172 None:intergenic 45.0%
ACTGCAAGTAATTCGGATCC+TGG + chr5_2:69898531-69898550 Msa0785090:three_prime_UTR 45.0%
! ATGCTAAGATCTAGGACAGG+TGG + chr5_2:69898748-69898767 Msa0785090:three_prime_UTR 45.0%
AGAACCTTCAAGCTAGTGGT+TGG + chr5_2:69899023-69899042 Msa0785090:three_prime_UTR 45.0%
! TAGACCAACCACTAGCTTGA+AGG - chr5_2:69899030-69899049 None:intergenic 45.0%
TTGATGCTGGATTTAGAGGC+TGG + chr5_2:69899107-69899126 Msa0785090:three_prime_UTR 45.0%
TGAGTTTGGCAAAATCACGG+TGG - chr5_2:69899349-69899368 None:intergenic 45.0%
! AAAGGTATGTCACTGTCCCA+TGG - chr5_2:69899799-69899818 None:intergenic 45.0%
GTCCAAAACCCTTAGAACTG+CGG - chr5_2:69899877-69899896 None:intergenic 45.0%
!!! AGTTCTAAGGGTTTTGGACG+CGG + chr5_2:69899878-69899897 Msa0785090:three_prime_UTR 45.0%
!! AAGGTAGGGAAAGAGCACCT+AGG - chr5_2:69897127-69897146 None:intergenic 50.0%
TCCCTACCTTCCCAAATCAC+CGG + chr5_2:69897137-69897156 Msa0785090:intron 50.0%
! GTTAAGCCGGTGATTTGGGA+AGG - chr5_2:69897146-69897165 None:intergenic 50.0%
GTGCAGTTAAGCCGGTGATT+TGG - chr5_2:69897151-69897170 None:intergenic 50.0%
TCTACAAGGAGTCTCTCAGC+TGG + chr5_2:69897508-69897527 Msa0785090:intron 50.0%
!! GCTGGTCTTCATTGCTGATG+AGG + chr5_2:69899125-69899144 Msa0785090:three_prime_UTR 50.0%
GTGACATGTTTGAGCCTTGG+AGG + chr5_2:69899657-69899676 Msa0785090:three_prime_UTR 50.0%
GTGTAGCTTCCACGTCAATC+AGG + chr5_2:69899844-69899863 Msa0785090:three_prime_UTR 50.0%
! GTGTTCTGCCGCAGTTCTAA+GGG + chr5_2:69899866-69899885 Msa0785090:three_prime_UTR 50.0%
!!! TGCCGCAGTTCTAAGGGTTT+TGG + chr5_2:69899872-69899891 Msa0785090:three_prime_UTR 50.0%
! GCCGGTGATTTGGGAAGGTA+GGG - chr5_2:69897141-69897160 None:intergenic 55.0%
! AGCCGGTGATTTGGGAAGGT+AGG - chr5_2:69897142-69897161 None:intergenic 55.0%
! AGGAGTCTCTCAGCTGGTTC+TGG + chr5_2:69897514-69897533 Msa0785090:intron 55.0%
! TGAGCCTTGGAGGAGAGCAT+AGG + chr5_2:69899667-69899686 Msa0785090:three_prime_UTR 55.0%
AGCACCTATGCTCTCCTCCA+AGG - chr5_2:69899674-69899693 None:intergenic 55.0%
! TTGGAGGAGAGCATAGGTGC+TGG + chr5_2:69899673-69899692 Msa0785090:three_prime_UTR 55.0%
GGTATGTCACTGTCCCATGG+CGG - chr5_2:69899796-69899815 None:intergenic 55.0%
GGCAGAACACCTGATTGACG+TGG - chr5_2:69899856-69899875 None:intergenic 55.0%
GGTGTTCTGCCGCAGTTCTA+AGG + chr5_2:69899865-69899884 Msa0785090:three_prime_UTR 55.0%
ACTGCGGGGTCAATTTCGTC+AGG + chr5_2:69900177-69900196 Msa0785090:three_prime_UTR 55.0%
! TGAGCGTTTTGCCACCGCCA+TGG + chr5_2:69899779-69899798 Msa0785090:three_prime_UTR 60.0%
! GAGCGTTTTGCCACCGCCAT+GGG + chr5_2:69899780-69899799 Msa0785090:three_prime_UTR 60.0%
ATGTCACTGTCCCATGGCGG+TGG - chr5_2:69899793-69899812 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 69897010 69900206 69897010 ID=Msa0785090;Name=Msa0785090
chr5_2 mRNA 69897010 69900206 69897010 ID=Msa0785090-mRNA-1;Parent=Msa0785090;Name=Msa0785090-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0.5|0.66|0.66|1|0.66|3|1519|151
chr5_2 exon 69897010 69897016 69897010 ID=Msa0785090-mRNA-1:exon:21082;Parent=Msa0785090-mRNA-1
chr5_2 exon 69897899 69899146 69897899 ID=Msa0785090-mRNA-1:exon:21083;Parent=Msa0785090-mRNA-1
chr5_2 exon 69899487 69900206 69899487 ID=Msa0785090-mRNA-1:exon:21084;Parent=Msa0785090-mRNA-1
chr5_2 CDS 69897010 69897016 69897010 ID=Msa0785090-mRNA-1:cds;Parent=Msa0785090-mRNA-1
chr5_2 CDS 69897899 69898347 69897899 ID=Msa0785090-mRNA-1:cds;Parent=Msa0785090-mRNA-1
chr5_2 three_prime_UTR 69898348 69899146 69898348 ID=Msa0785090-mRNA-1:three_prime_utr;Parent=Msa0785090-mRNA-1
chr5_2 three_prime_UTR 69899487 69900206 69899487 ID=Msa0785090-mRNA-1:three_prime_utr;Parent=Msa0785090-mRNA-1
Gene Sequence

>Msa0785090

ATGGCACAGATGGGGAGTAGTTTTGTGCTTGAATCAGGTTTCTACATCACATCATTTTCAGCAACAATCTTCATTGCTGGATTTGCAGCTCTTGGACTTTTATTAGTCACTTTGTTGGTTTCAATGGCAATGATGCTACAATCATGTCAAAATAGCAATGCTGGAATTATTGAGCTTAAGAATATAAATGATGACTATAGTTATTGCAAAACTCATTCTCTGCATGCTAAGCTCAATAATTTGGAAGAACATAATGTTCCTAACATTTGTAAGGACCTGGCTTTACAATATATCAAAGGTGGTCAATATGCAAGAGACTTGGATTCAACCAAATCTGTGATTGAGGATTACCTCAATGGTGTTAAACCATCAGAAGATGGTTTTGATGCGGTGTTGATAGACATAGATAGCCTTTTTCAATGGAATTCTCATTCTTCCAATTTGTTACTAAGGTAA
Protein sequence

>Msa0785090

MAQMGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNAGIIELKNINDDYSYCKTHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYLNGVKPSEDGFDAVLIDIDSLFQWNSHSSNLLLR*