AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0787700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787700 MtrunA17_Chr1g0199571 92.308 78 6 0 9 86 127 204 3.11e-42 147
Msa0787700 MtrunA17_Chr4g0049851 72.165 97 20 1 84 173 38 134 1.36e-40 141
Msa0787700 MtrunA17_Chr4g0049861 58.763 97 33 1 84 173 36 132 3.91e-32 119
Msa0787700 MtrunA17_Chr1g0156811 48.515 101 51 1 3 103 121 220 1.37e-26 104
Msa0787700 MtrunA17_Chr4g0037111 53.571 84 39 0 3 86 121 204 1.74e-25 100
Msa0787700 MtrunA17_Chr7g0246311 32.990 97 63 2 8 103 124 219 7.85e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787700 AT5G04220.3 54.737 95 42 1 9 103 65 158 4.86e-28 108
Msa0787700 AT5G04220.2 54.737 95 42 1 9 103 127 220 5.89e-28 108
Msa0787700 AT2G20990.3 54.762 84 38 0 3 86 121 204 1.86e-25 102
Msa0787700 AT2G20990.1 54.762 84 38 0 3 86 121 204 1.99e-25 102
Msa0787700 AT2G20990.2 54.762 84 38 0 3 86 121 204 2.01e-25 102
Msa0787700 AT1G20080.2 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa0787700 AT1G20080.3 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa0787700 AT1G20080.4 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa0787700 AT1G20080.1 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa0787700 AT1G20080.5 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa0787700 AT5G64370.1 55.556 90 33 1 84 166 32 121 4.42e-23 94.7
Msa0787700 AT3G61050.2 32.990 97 63 2 8 103 124 219 4.76e-11 60.8
Msa0787700 AT3G61050.1 32.990 97 63 2 8 103 124 219 4.76e-11 60.8

Find 117 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATCATGCTGACAGTTTC+TGG 0.155569 5_2:+73661203 Msa0787700:three_prime_UTR
TATTATTGTTATGGATTATT+AGG 0.158712 5_2:-73661146 None:intergenic
TATTAGGACTGCTTAATTTA+AGG 0.161510 5_2:-73661130 None:intergenic
TAGTGTTGAGATTCCTATTA+AGG 0.189939 5_2:+73659523 Msa0787700:five_prime_UTR
ATGCTGATAAAGAAGTATTA+AGG 0.197682 5_2:+73660636 Msa0787700:CDS
TAGCTTATGAAATATGATTT+TGG 0.224472 5_2:+73658907 Msa0787700:five_prime_UTR
TCAAAGTTGAGAGGCTAAAT+TGG 0.241187 5_2:-73659147 None:intergenic
GATATTCCTTCATTTGATTT+AGG 0.246878 5_2:+73661534 Msa0787700:three_prime_UTR
TTATATGGATAATTAAATTA+TGG 0.259071 5_2:-73661662 None:intergenic
TTCTGCAGGTATGAAAGTTT+TGG 0.273201 5_2:+73659803 Msa0787700:intron
AACAATGCCAGTTTAACTTT+TGG 0.280661 5_2:-73659561 None:intergenic
TTTGGAGCAACACCCTTAAT+AGG 0.284160 5_2:-73659536 None:intergenic
ACTCTCAATTGGAAGTTATA+TGG 0.289029 5_2:-73661677 None:intergenic
TGCAAGGTCAATACTATGTT+TGG 0.291324 5_2:-73659879 None:intergenic
TCCATAACAATAATAGTAAA+AGG 0.311165 5_2:+73661154 Msa0787700:three_prime_UTR
TCTAACTTTCATCACATTCA+TGG 0.315827 5_2:-73659199 None:intergenic
TGTTGTTTGGTTCTCTGTTT+AGG 0.321059 5_2:+73662054 Msa0787700:three_prime_UTR
GCTAAGATCAGCAAAATTTC+AGG 0.326164 5_2:+73659614 Msa0787700:CDS
ATTATTGAAGGATAGTTAAC+TGG 0.331648 5_2:+73660875 Msa0787700:three_prime_UTR
GAGAGAGCTGTGGCGGATTC+CGG 0.340791 5_2:-73660350 None:intergenic
GGAAACAAATGAAAAGGAAT+TGG 0.343840 5_2:+73659824 Msa0787700:CDS
TATTCTGCAATGGTTGAATC+AGG 0.347023 5_2:-73661593 None:intergenic
CCAAAATCCAAAAGTTAAAC+TGG 0.349476 5_2:+73659554 Msa0787700:five_prime_UTR
GCAGATAAGTTTAAGACCTT+TGG 0.357838 5_2:+73660043 Msa0787700:CDS
ATATAATTTACCATGTTGTT+TGG 0.362994 5_2:+73658833 Msa0787700:five_prime_UTR
ATGGTTGATTTGGTGTTGTT+TGG 0.367363 5_2:+73662041 Msa0787700:three_prime_UTR
ATGTCTTCCAGCCTGTATAA+AGG 0.372001 5_2:+73660791 Msa0787700:three_prime_UTR
TCGATAAAGTGCGCAATTGT+TGG 0.373558 5_2:-73660707 None:intergenic
GTTTATGGGAGCATGAATTA+TGG 0.389437 5_2:+73661771 Msa0787700:three_prime_UTR
AAAAGTTAAACTGGCATTGT+TGG 0.398909 5_2:+73659563 Msa0787700:five_prime_UTR
CGATCTATATGCTGCGATCT+TGG 0.403507 5_2:+73661828 Msa0787700:three_prime_UTR
AGTGTTGAGATTCCTATTAA+GGG 0.409795 5_2:+73659524 Msa0787700:five_prime_UTR
AAAGATTGTGGTGTCTTTGA+TGG 0.416050 5_2:+73660088 Msa0787700:CDS
CTTCATAACCACATTATTGA+AGG 0.418430 5_2:+73660863 Msa0787700:three_prime_UTR
GTGTAGCGGAGGCAGGGAGG+TGG 0.422043 5_2:+73659035 Msa0787700:five_prime_UTR
AGACATTGGGATGATGGGTT+TGG 0.424062 5_2:+73659124 Msa0787700:five_prime_UTR
TGCTGATAAAGAAGTATTAA+GGG 0.432567 5_2:+73660637 Msa0787700:CDS
TGTCTTCCAGCCTGTATAAA+GGG 0.435209 5_2:+73660792 Msa0787700:three_prime_UTR
TATTGCATTCCTTGCAATGA+TGG 0.437005 5_2:-73659101 None:intergenic
GCAATAAGACATTGGGATGA+TGG 0.439230 5_2:+73659118 Msa0787700:five_prime_UTR
TTGCTAACTATAGAAAGAAA+TGG 0.440469 5_2:+73659241 Msa0787700:five_prime_UTR
AAACTTAGGTCTGGATTTGA+AGG 0.442436 5_2:-73661913 None:intergenic
GAGAGTGATGCTTTCTAAGA+TGG 0.442565 5_2:+73661270 Msa0787700:three_prime_UTR
GCAAGGTCAATACTATGTTT+GGG 0.443699 5_2:-73659878 None:intergenic
CGATAAAGTGCGCAATTGTT+GGG 0.445360 5_2:-73660706 None:intergenic
ATGTCTGGTGTAGCGGAGGC+AGG 0.450530 5_2:+73659028 Msa0787700:five_prime_UTR
TAATGAAACAATTGCTCTAC+CGG 0.451479 5_2:+73660331 Msa0787700:CDS
CACTAACCACGCTGCAATCA+TGG 0.461908 5_2:-73661883 None:intergenic
TCATGAAACAAGTCATCAAA+TGG 0.463122 5_2:+73659847 Msa0787700:CDS
ATGTATCATAAGCATATTGT+TGG 0.476359 5_2:+73661237 Msa0787700:three_prime_UTR
ATCCATTGGTCTTCTTGCAT+AGG 0.483976 5_2:-73661491 None:intergenic
TACAGAATTTAGAGTCGTAA+AGG 0.487741 5_2:-73661355 None:intergenic
CAAGGAATGCAATAAGACAT+TGG 0.491395 5_2:+73659110 Msa0787700:five_prime_UTR
ACGAAAGAAGTTGCATCCAT+TGG 0.491832 5_2:-73661505 None:intergenic
AAGGAATGCAATAAGACATT+GGG 0.493762 5_2:+73659111 Msa0787700:five_prime_UTR
TATGAGATTGAATTTATGTC+TGG 0.494249 5_2:+73659013 Msa0787700:five_prime_UTR
TTAACAACAGAGTTCTGAAT+GGG 0.497423 5_2:-73660761 None:intergenic
TGTAGCGGAGGCAGGGAGGT+GGG 0.498715 5_2:+73659036 Msa0787700:five_prime_UTR
CAATAAGACATTGGGATGAT+GGG 0.504343 5_2:+73659119 Msa0787700:five_prime_UTR
TGATGCTAGACCCTTTATAC+AGG 0.508168 5_2:-73660802 None:intergenic
GCTACAGACGGAGGAAACTT+AGG 0.512242 5_2:-73661927 None:intergenic
GCTAGACCCTTTATACAGGC+TGG 0.518050 5_2:-73660798 None:intergenic
AGACGGAGGAAACTTAGGTC+TGG 0.518668 5_2:-73661922 None:intergenic
AGAGTGATGCTTTCTAAGAT+GGG 0.525015 5_2:+73661271 Msa0787700:three_prime_UTR
ACCACAATCTTTGCAAAACA+TGG 0.526489 5_2:-73660077 None:intergenic
TGACTACCTAAATCAAATGA+AGG 0.528716 5_2:-73661540 None:intergenic
GTCTATGAAGATCAAAGTGC+AGG 0.534460 5_2:+73659908 Msa0787700:CDS
AGCACATTGTTATTCTGCAA+TGG 0.534464 5_2:-73661603 None:intergenic
GTATAAAGGGTCTAGCATCA+TGG 0.539096 5_2:+73660805 Msa0787700:three_prime_UTR
ATCCGCCACAGCTCTCTCCA+TGG 0.543560 5_2:+73660355 Msa0787700:CDS
AACAGCTATAAGTAGATCCT+CGG 0.546715 5_2:-73661451 None:intergenic
TATAAAGGGTCTAGCATCAT+GGG 0.551527 5_2:+73660806 Msa0787700:three_prime_UTR
AAGGGAACCTCGAGTTGTGA+GGG 0.552694 5_2:+73660655 Msa0787700:CDS
TTAACTATCCTTCAATAATG+TGG 0.555394 5_2:-73660871 None:intergenic
TCAATTAATTCAAAGTTGAG+AGG 0.557443 5_2:-73659156 None:intergenic
AATTATGGTCCTGGTGTCAA+TGG 0.558417 5_2:+73661786 Msa0787700:three_prime_UTR
TGAATTTATGTCTGGTGTAG+CGG 0.559041 5_2:+73659021 Msa0787700:five_prime_UTR
GAACCTCGAGTTGTGAGGGT+TGG 0.560787 5_2:+73660659 Msa0787700:CDS
GTTAACAACAGAGTTCTGAA+TGG 0.561492 5_2:-73660762 None:intergenic
TGTGGTGTCTTTGATGGAGA+AGG 0.563558 5_2:+73660094 Msa0787700:CDS
TGGAGTGGCTAATATATGAC+AGG 0.565325 5_2:-73661716 None:intergenic
AAACAAGTCATCAAATGGAC+TGG 0.565498 5_2:+73659852 Msa0787700:CDS
GCTCTGCAGATTTATTTACC+AGG 0.565646 5_2:+73658798 Msa0787700:five_prime_UTR
TTTAGGTCTTGACAGTGGAG+TGG 0.576655 5_2:+73662071 Msa0787700:three_prime_UTR
GAGGTCAAAACCATGTTCCA+TGG 0.583571 5_2:-73660372 None:intergenic
TGTCTGGTGTAGCGGAGGCA+GGG 0.584181 5_2:+73659029 Msa0787700:five_prime_UTR
AGCCTATGCAAGAAGACCAA+TGG 0.586752 5_2:+73661489 Msa0787700:three_prime_UTR
CTCTGTTTAGGTCTTGACAG+TGG 0.588161 5_2:+73662066 Msa0787700:three_prime_UTR
TTGCAGCGTGGTTAGTGTGT+TGG 0.592356 5_2:+73661889 Msa0787700:three_prime_UTR
TTCCATGGAGAGAGCTGTGG+CGG 0.594000 5_2:-73660357 None:intergenic
GGGAGCATGAATTATGGTCC+TGG 0.596278 5_2:+73661777 Msa0787700:three_prime_UTR
TCTTTCTATAGTTAGCAAAG+TGG 0.597790 5_2:-73659236 None:intergenic
ACAGCTCTCTCCATGGAACA+TGG 0.598176 5_2:+73660362 Msa0787700:CDS
TAAGGGAACCTCGAGTTGTG+AGG 0.598332 5_2:+73660654 Msa0787700:CDS
GGATGCAACTTCTTTCGTCA+AGG 0.598987 5_2:+73661510 Msa0787700:three_prime_UTR
TTTGCATGATTTGGCCTCCG+AGG 0.600110 5_2:+73661434 Msa0787700:three_prime_UTR
CTGGTGTAGCGGAGGCAGGG+AGG 0.603912 5_2:+73659032 Msa0787700:five_prime_UTR
AAAGTGCGCAATTGTTGGGA+GGG 0.605427 5_2:-73660702 None:intergenic
AACTTAGGTCTGGATTTGAA+GGG 0.615955 5_2:-73661912 None:intergenic
AAACCAACCCTCACAACTCG+AGG 0.620062 5_2:-73660662 None:intergenic
CTGGTGTCAATGGTAGAAGT+TGG 0.621412 5_2:+73661796 Msa0787700:three_prime_UTR
TAAAGTGCGCAATTGTTGGG+AGG 0.630263 5_2:-73660703 None:intergenic
ATTTATGTCTGGTGTAGCGG+AGG 0.641352 5_2:+73659024 Msa0787700:five_prime_UTR
AAAGGTCTTAAACTTATCTG+CGG 0.650638 5_2:-73660041 None:intergenic
CAACTTCTACCATTGACACC+AGG 0.652303 5_2:-73661795 None:intergenic
CTTCATAGACAACACATGCA+AGG 0.656238 5_2:-73659895 None:intergenic
TCTACTGCACCATCATTGCA+AGG 0.665556 5_2:+73659092 Msa0787700:five_prime_UTR
ATGTTCCATGGAGAGAGCTG+TGG 0.669038 5_2:-73660360 None:intergenic
AATTAAATTATGGCAAAGAG+CGG 0.674865 5_2:-73661652 None:intergenic
CATGGAAGTGTAGACACCAA+AGG 0.678495 5_2:-73660059 None:intergenic
GTGAACATAAGCGAAATGCA+AGG 0.684278 5_2:+73659590 Msa0787700:exon
TTCTTCGATACCAAACAACA+TGG 0.687542 5_2:-73658843 None:intergenic
CTACCACCACTAAAACAGCA+TGG 0.690939 5_2:-73661976 None:intergenic
AGCTATAAGTAGATCCTCGG+AGG 0.708824 5_2:-73661448 None:intergenic
AAAGGGTCTAGCATCATGGG+AGG 0.713742 5_2:+73660809 Msa0787700:three_prime_UTR
ACACGCCGAAGAAAACTGTG+AGG 0.784742 5_2:-73659069 None:intergenic
TTGTTGCCATGATTGCAGCG+TGG 0.786597 5_2:+73661877 Msa0787700:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATGTTTTGTTTTT+AGG + chr5_2:73659399-73659418 Msa0787700:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG + chr5_2:73659400-73659419 Msa0787700:intron 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG + chr5_2:73659399-73659418 Msa0787700:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG + chr5_2:73659400-73659419 Msa0787700:intron 10.0%
!! AATTATTTATCAAATAAGAA+GGG - chr5_2:73660192-73660211 None:intergenic 10.0%
!! TAATTATTTATCAAATAAGA+AGG - chr5_2:73660193-73660212 None:intergenic 10.0%
!!! ATTTTAAGTTTATTTATTTC+AGG + chr5_2:73660601-73660620 Msa0787700:intron 10.0%
!! TTATATGGATAATTAAATTA+TGG - chr5_2:73661665-73661684 None:intergenic 10.0%
!! GTTAAATAAAAAAAAGTTTA+TGG + chr5_2:73661756-73661775 Msa0787700:three_prime_UTR 10.0%
!! AACATAATAATAAGAATGAT+TGG - chr5_2:73659474-73659493 None:intergenic 15.0%
!!! TATTATTGTTATGGATTATT+AGG - chr5_2:73661149-73661168 None:intergenic 15.0%
!! ATATAATTTACCATGTTGTT+TGG + chr5_2:73658833-73658852 Msa0787700:five_prime_UTR 20.0%
!!! TAGCTTATGAAATATGATTT+TGG + chr5_2:73658907-73658926 Msa0787700:five_prime_UTR 20.0%
!!! ACAAAATGATTTTGTGATAA+TGG + chr5_2:73659291-73659310 Msa0787700:intron 20.0%
!! TTTATCTTGTGATATCATTA+GGG - chr5_2:73660423-73660442 None:intergenic 20.0%
!!! TTTTATCTTGTGATATCATT+AGG - chr5_2:73660424-73660443 None:intergenic 20.0%
!! TAATGATATCACAAGATAAA+AGG + chr5_2:73660423-73660442 Msa0787700:intron 20.0%
!!! GTTATTTAAGCTATAACTTT+TGG + chr5_2:73660567-73660586 Msa0787700:intron 20.0%
!! TGTTTACTTAAAGAAAAAAC+GGG - chr5_2:73660918-73660937 None:intergenic 20.0%
!! GTGTTTACTTAAAGAAAAAA+CGG - chr5_2:73660919-73660938 None:intergenic 20.0%
!!! TTTTTCTTTAAGTAAACACA+TGG + chr5_2:73660920-73660939 Msa0787700:three_prime_UTR 20.0%
!! AACCAAAGAAAAAAAAAAGA+AGG + chr5_2:73661102-73661121 Msa0787700:three_prime_UTR 20.0%
!!! TACCTTCTTTTTTTTTTCTT+TGG - chr5_2:73661107-73661126 None:intergenic 20.0%
!! TCCATAACAATAATAGTAAA+AGG + chr5_2:73661154-73661173 Msa0787700:three_prime_UTR 20.0%
!!! ACCTTTTACTATTATTGTTA+TGG - chr5_2:73661158-73661177 None:intergenic 20.0%
!!! TGTGTTTTATTTCTGTTTTA+TGG + chr5_2:73662022-73662041 Msa0787700:three_prime_UTR 20.0%
!!! TTATATTTTGAACTCTAACC+TGG - chr5_2:73658819-73658838 None:intergenic 25.0%
!! AGTTATTGTGTTTTGTCATT+TGG + chr5_2:73658930-73658949 Msa0787700:five_prime_UTR 25.0%
!! TTTCTTCAAGATTTTGTGTT+TGG + chr5_2:73658971-73658990 Msa0787700:five_prime_UTR 25.0%
! TATGAGATTGAATTTATGTC+TGG + chr5_2:73659013-73659032 Msa0787700:five_prime_UTR 25.0%
! TCAATTAATTCAAAGTTGAG+AGG - chr5_2:73659159-73659178 None:intergenic 25.0%
! TTGCTAACTATAGAAAGAAA+TGG + chr5_2:73659241-73659260 Msa0787700:five_prime_UTR 25.0%
!!! TAAGTGTCATTTAGTGATAT+TGG + chr5_2:73659327-73659346 Msa0787700:intron 25.0%
!!! AAGTGTCATTTAGTGATATT+GGG + chr5_2:73659328-73659347 Msa0787700:intron 25.0%
! TGTAAAGCTTTCAAAATACA+AGG + chr5_2:73659655-73659674 Msa0787700:intron 25.0%
! TAATCCTCTCTTTCATTTAT+AGG + chr5_2:73659742-73659761 Msa0787700:intron 25.0%
! AATCCTCTCTTTCATTTATA+GGG + chr5_2:73659743-73659762 Msa0787700:intron 25.0%
!!! AGTTTTGGAAACAAATGAAA+AGG + chr5_2:73659818-73659837 Msa0787700:CDS 25.0%
!! TATTTTCTAATTTTCGGTGA+GGG - chr5_2:73659947-73659966 None:intergenic 25.0%
!! TTATTTTCTAATTTTCGGTG+AGG - chr5_2:73659948-73659967 None:intergenic 25.0%
!!! TGATTTTGATTTTTCTCAGT+TGG + chr5_2:73659995-73660014 Msa0787700:intron 25.0%
! TTTCTTCTAATTTCACTTCT+TGG + chr5_2:73660133-73660152 Msa0787700:intron 25.0%
! TTCTTCTAATTTCACTTCTT+GGG + chr5_2:73660134-73660153 Msa0787700:intron 25.0%
! CTACAATATAATCATAATCG+CGG - chr5_2:73660282-73660301 None:intergenic 25.0%
!!! ATGCTGATAAAGAAGTATTA+AGG + chr5_2:73660636-73660655 Msa0787700:CDS 25.0%
!!! TGCTGATAAAGAAGTATTAA+GGG + chr5_2:73660637-73660656 Msa0787700:CDS 25.0%
! TTAACTATCCTTCAATAATG+TGG - chr5_2:73660874-73660893 None:intergenic 25.0%
! ATTATTGAAGGATAGTTAAC+TGG + chr5_2:73660875-73660894 Msa0787700:three_prime_UTR 25.0%
! TATTAGGACTGCTTAATTTA+AGG - chr5_2:73661133-73661152 None:intergenic 25.0%
! ATGTATCATAAGCATATTGT+TGG + chr5_2:73661237-73661256 Msa0787700:three_prime_UTR 25.0%
!! GATATTCCTTCATTTGATTT+AGG + chr5_2:73661534-73661553 Msa0787700:three_prime_UTR 25.0%
! AATTAAATTATGGCAAAGAG+CGG - chr5_2:73661655-73661674 None:intergenic 25.0%
! AGCTTAAAAAAACTCTCAAT+TGG - chr5_2:73661691-73661710 None:intergenic 25.0%
!! TTTCTGTTTTATGGTTGATT+TGG + chr5_2:73662031-73662050 Msa0787700:three_prime_UTR 25.0%
! AAGATTTTGTGTTTGGTCAT+TGG + chr5_2:73658978-73658997 Msa0787700:five_prime_UTR 30.0%
AAGGAATGCAATAAGACATT+GGG + chr5_2:73659111-73659130 Msa0787700:five_prime_UTR 30.0%
TCTAACTTTCATCACATTCA+TGG - chr5_2:73659202-73659221 None:intergenic 30.0%
TCTTTCTATAGTTAGCAAAG+TGG - chr5_2:73659239-73659258 None:intergenic 30.0%
AATGGTACGATAGATAAGTA+AGG + chr5_2:73659259-73659278 Msa0787700:intron 30.0%
ATGGTACGATAGATAAGTAA+GGG + chr5_2:73659260-73659279 Msa0787700:intron 30.0%
!! AGTGTCATTTAGTGATATTG+GGG + chr5_2:73659329-73659348 Msa0787700:intron 30.0%
AAGAATGAAAGTGATGATCA+TGG - chr5_2:73659380-73659399 None:intergenic 30.0%
TAGTGTTGTGATTCCTATTA+AGG + chr5_2:73659440-73659459 Msa0787700:intron 30.0%
AGTGTTGTGATTCCTATTAA+GGG + chr5_2:73659441-73659460 Msa0787700:intron 30.0%
! TAGTGTTGAGATTCCTATTA+AGG + chr5_2:73659523-73659542 Msa0787700:five_prime_UTR 30.0%
! AGTGTTGAGATTCCTATTAA+GGG + chr5_2:73659524-73659543 Msa0787700:five_prime_UTR 30.0%
!! CCAGTTTAACTTTTGGATTT+TGG - chr5_2:73659557-73659576 None:intergenic 30.0%
CCAAAATCCAAAAGTTAAAC+TGG + chr5_2:73659554-73659573 Msa0787700:five_prime_UTR 30.0%
!! AACAATGCCAGTTTAACTTT+TGG - chr5_2:73659564-73659583 None:intergenic 30.0%
AAAAGTTAAACTGGCATTGT+TGG + chr5_2:73659563-73659582 Msa0787700:five_prime_UTR 30.0%
! CTTTGTTTCTTTGATGTGTT+TGG + chr5_2:73659695-73659714 Msa0787700:intron 30.0%
ATCCTCTCTTTCATTTATAG+GGG + chr5_2:73659744-73659763 Msa0787700:intron 30.0%
GGAAACAAATGAAAAGGAAT+TGG + chr5_2:73659824-73659843 Msa0787700:CDS 30.0%
TCATGAAACAAGTCATCAAA+TGG + chr5_2:73659847-73659866 Msa0787700:CDS 30.0%
AAAGGTCTTAAACTTATCTG+CGG - chr5_2:73660044-73660063 None:intergenic 30.0%
TAATGAAACAATTGCTCTAC+CGG + chr5_2:73660331-73660350 Msa0787700:CDS 30.0%
!! TTAACAACAGAGTTCTGAAT+GGG - chr5_2:73660764-73660783 None:intergenic 30.0%
CTTCATAACCACATTATTGA+AGG + chr5_2:73660863-73660882 Msa0787700:three_prime_UTR 30.0%
!! ATCTTGTGTCTACTTTTCTT+TGG - chr5_2:73661061-73661080 None:intergenic 30.0%
! TACAGAATTTAGAGTCGTAA+AGG - chr5_2:73661358-73661377 None:intergenic 30.0%
! TTTTGTCTCTTTGCATGATT+TGG + chr5_2:73661425-73661444 Msa0787700:three_prime_UTR 30.0%
TGACTACCTAAATCAAATGA+AGG - chr5_2:73661543-73661562 None:intergenic 30.0%
ACTCTCAATTGGAAGTTATA+TGG - chr5_2:73661680-73661699 None:intergenic 30.0%
AACGAAAAAAATGTCTTCTG+TGG - chr5_2:73661739-73661758 None:intergenic 30.0%
!!! CGATCTTGGTTATTTTTACA+AGG + chr5_2:73661842-73661861 Msa0787700:three_prime_UTR 30.0%
TTCTTCGATACCAAACAACA+TGG - chr5_2:73658846-73658865 None:intergenic 35.0%
TGAATTTATGTCTGGTGTAG+CGG + chr5_2:73659021-73659040 Msa0787700:five_prime_UTR 35.0%
TATTGCATTCCTTGCAATGA+TGG - chr5_2:73659104-73659123 None:intergenic 35.0%
CAAGGAATGCAATAAGACAT+TGG + chr5_2:73659110-73659129 Msa0787700:five_prime_UTR 35.0%
CAATAAGACATTGGGATGAT+GGG + chr5_2:73659119-73659138 Msa0787700:five_prime_UTR 35.0%
TCAAAGTTGAGAGGCTAAAT+TGG - chr5_2:73659150-73659169 None:intergenic 35.0%
GCTAAGATCAGCAAAATTTC+AGG + chr5_2:73659614-73659633 Msa0787700:CDS 35.0%
ACAAAGCAACAAAATCACAG+AGG - chr5_2:73659681-73659700 None:intergenic 35.0%
TACCCCTATAAATGAAAGAG+AGG - chr5_2:73659749-73659768 None:intergenic 35.0%
ATTGTTTGTAGCAATTCTGC+AGG + chr5_2:73659789-73659808 Msa0787700:intron 35.0%
! TTCTGCAGGTATGAAAGTTT+TGG + chr5_2:73659803-73659822 Msa0787700:intron 35.0%
AAACAAGTCATCAAATGGAC+TGG + chr5_2:73659852-73659871 Msa0787700:CDS 35.0%
GCAAGGTCAATACTATGTTT+GGG - chr5_2:73659881-73659900 None:intergenic 35.0%
TGCAAGGTCAATACTATGTT+TGG - chr5_2:73659882-73659901 None:intergenic 35.0%
! GCAGATAAGTTTAAGACCTT+TGG + chr5_2:73660043-73660062 Msa0787700:CDS 35.0%
! TCCATGTTTTGCAAAGATTG+TGG + chr5_2:73660076-73660095 Msa0787700:CDS 35.0%
ACCACAATCTTTGCAAAACA+TGG - chr5_2:73660080-73660099 None:intergenic 35.0%
AAAGATTGTGGTGTCTTTGA+TGG + chr5_2:73660088-73660107 Msa0787700:CDS 35.0%
TGAAGTGTATTCATACCTTG+AGG - chr5_2:73660394-73660413 None:intergenic 35.0%
! TCACAAGATAAAAGGTATGC+TGG + chr5_2:73660431-73660450 Msa0787700:intron 35.0%
ACAGATAAGTTTCAATGCTG+AGG - chr5_2:73660549-73660568 None:intergenic 35.0%
GCACTTTATCGAGCAAAAAA+AGG + chr5_2:73660718-73660737 Msa0787700:CDS 35.0%
!! GTTAACAACAGAGTTCTGAA+TGG - chr5_2:73660765-73660784 None:intergenic 35.0%
TATAAAGGGTCTAGCATCAT+GGG + chr5_2:73660806-73660825 Msa0787700:three_prime_UTR 35.0%
! TAACTGGATTTTGCCAAGTT+AGG + chr5_2:73660891-73660910 Msa0787700:three_prime_UTR 35.0%
! AACTGGATTTTGCCAAGTTA+GGG + chr5_2:73660892-73660911 Msa0787700:three_prime_UTR 35.0%
ATAATCATGCTGACAGTTTC+TGG + chr5_2:73661203-73661222 Msa0787700:three_prime_UTR 35.0%
!! AGAGTGATGCTTTCTAAGAT+GGG + chr5_2:73661271-73661290 Msa0787700:three_prime_UTR 35.0%
AACAGCTATAAGTAGATCCT+CGG - chr5_2:73661454-73661473 None:intergenic 35.0%
TATTCTGCAATGGTTGAATC+AGG - chr5_2:73661596-73661615 None:intergenic 35.0%
! AGCACATTGTTATTCTGCAA+TGG - chr5_2:73661606-73661625 None:intergenic 35.0%
AAAAAATGTCTTCTGTGGAG+TGG - chr5_2:73661734-73661753 None:intergenic 35.0%
! GTTTATGGGAGCATGAATTA+TGG + chr5_2:73661771-73661790 Msa0787700:three_prime_UTR 35.0%
AACTTAGGTCTGGATTTGAA+GGG - chr5_2:73661915-73661934 None:intergenic 35.0%
AAACTTAGGTCTGGATTTGA+AGG - chr5_2:73661916-73661935 None:intergenic 35.0%
!! ATGGTTGATTTGGTGTTGTT+TGG + chr5_2:73662041-73662060 Msa0787700:three_prime_UTR 35.0%
!! TGTTGTTTGGTTCTCTGTTT+AGG + chr5_2:73662054-73662073 Msa0787700:three_prime_UTR 35.0%
GCTCTGCAGATTTATTTACC+AGG + chr5_2:73658798-73658817 Msa0787700:five_prime_UTR 40.0%
! GCAATCCTCACAGTTTTCTT+CGG + chr5_2:73659064-73659083 Msa0787700:five_prime_UTR 40.0%
GCAATAAGACATTGGGATGA+TGG + chr5_2:73659118-73659137 Msa0787700:five_prime_UTR 40.0%
GTCATTTAGTGATATTGGGG+TGG + chr5_2:73659332-73659351 Msa0787700:intron 40.0%
ATTGGAGCAACACCCTTAAT+AGG - chr5_2:73659456-73659475 None:intergenic 40.0%
! TTTGGAGCAACACCCTTAAT+AGG - chr5_2:73659539-73659558 None:intergenic 40.0%
GTGAACATAAGCGAAATGCA+AGG + chr5_2:73659590-73659609 Msa0787700:exon 40.0%
CTTCATAGACAACACATGCA+AGG - chr5_2:73659898-73659917 None:intergenic 40.0%
GTCTATGAAGATCAAAGTGC+AGG + chr5_2:73659908-73659927 Msa0787700:CDS 40.0%
! TTCTAATTTTCGGTGAGGGT+AGG - chr5_2:73659943-73659962 None:intergenic 40.0%
TGTCTTTGATGGAGAAGGTA+AGG + chr5_2:73660099-73660118 Msa0787700:intron 40.0%
GTCTTTGATGGAGAAGGTAA+GGG + chr5_2:73660100-73660119 Msa0787700:intron 40.0%
GTTCAATTTACAGCCTCATG+TGG + chr5_2:73660214-73660233 Msa0787700:intron 40.0%
! TTACAGCCTCATGTGGATTT+TGG + chr5_2:73660221-73660240 Msa0787700:intron 40.0%
! TACAGCCTCATGTGGATTTT+GGG + chr5_2:73660222-73660241 Msa0787700:intron 40.0%
GCATACACTCCATCAGTTTA+AGG - chr5_2:73660491-73660510 None:intergenic 40.0%
CGATAAAGTGCGCAATTGTT+GGG - chr5_2:73660709-73660728 None:intergenic 40.0%
TCGATAAAGTGCGCAATTGT+TGG - chr5_2:73660710-73660729 None:intergenic 40.0%
ATGTCTTCCAGCCTGTATAA+AGG + chr5_2:73660791-73660810 Msa0787700:three_prime_UTR 40.0%
TGTCTTCCAGCCTGTATAAA+GGG + chr5_2:73660792-73660811 Msa0787700:three_prime_UTR 40.0%
TGATGCTAGACCCTTTATAC+AGG - chr5_2:73660805-73660824 None:intergenic 40.0%
GTATAAAGGGTCTAGCATCA+TGG + chr5_2:73660805-73660824 Msa0787700:three_prime_UTR 40.0%
! GAGAGTGATGCTTTCTAAGA+TGG + chr5_2:73661270-73661289 Msa0787700:three_prime_UTR 40.0%
!! ATCCATTGGTCTTCTTGCAT+AGG - chr5_2:73661494-73661513 None:intergenic 40.0%
ACGAAAGAAGTTGCATCCAT+TGG - chr5_2:73661508-73661527 None:intergenic 40.0%
TGGAGTGGCTAATATATGAC+AGG - chr5_2:73661719-73661738 None:intergenic 40.0%
! AATTATGGTCCTGGTGTCAA+TGG + chr5_2:73661786-73661805 Msa0787700:three_prime_UTR 40.0%
CCACAAAAAGTTGCTACAGA+CGG - chr5_2:73661942-73661961 None:intergenic 40.0%
! TGTAGCAACTTTTTGTGGTC+AGG + chr5_2:73661944-73661963 Msa0787700:three_prime_UTR 40.0%
ATTTATGTCTGGTGTAGCGG+AGG + chr5_2:73659024-73659043 Msa0787700:five_prime_UTR 45.0%
TCTACTGCACCATCATTGCA+AGG + chr5_2:73659092-73659111 Msa0787700:five_prime_UTR 45.0%
!! AGACATTGGGATGATGGGTT+TGG + chr5_2:73659124-73659143 Msa0787700:five_prime_UTR 45.0%
! CATGGAAGTGTAGACACCAA+AGG - chr5_2:73660062-73660081 None:intergenic 45.0%
TGTGGTGTCTTTGATGGAGA+AGG + chr5_2:73660094-73660113 Msa0787700:CDS 45.0%
!!! GATTTTGGGTTGGCGATATC+TGG + chr5_2:73660236-73660255 Msa0787700:intron 45.0%
GAGGTCAAAACCATGTTCCA+TGG - chr5_2:73660375-73660394 None:intergenic 45.0%
!!! GGAACATGGTTTTGACCTCA+AGG + chr5_2:73660376-73660395 Msa0787700:CDS 45.0%
!! GCTGTGTTGCTTCCTTAAAC+TGG + chr5_2:73660463-73660482 Msa0787700:intron 45.0%
! CAGTTTAAGGCGCCAGTTTA+AGG - chr5_2:73660478-73660497 None:intergenic 45.0%
AAACTGGCGCCTTAAACTGA+TGG + chr5_2:73660479-73660498 Msa0787700:intron 45.0%
TGCTCGATTTCTCGACAACA+CGG + chr5_2:73660508-73660527 Msa0787700:intron 45.0%
AAAGTGCGCAATTGTTGGGA+GGG - chr5_2:73660705-73660724 None:intergenic 45.0%
TAAAGTGCGCAATTGTTGGG+AGG - chr5_2:73660706-73660725 None:intergenic 45.0%
AGAAAAAACGGGCCCTAACT+TGG - chr5_2:73660907-73660926 None:intergenic 45.0%
AGCTATAAGTAGATCCTCGG+AGG - chr5_2:73661451-73661470 None:intergenic 45.0%
AGCCTATGCAAGAAGACCAA+TGG + chr5_2:73661489-73661508 Msa0787700:three_prime_UTR 45.0%
GGATGCAACTTCTTTCGTCA+AGG + chr5_2:73661510-73661529 Msa0787700:three_prime_UTR 45.0%
CAACTTCTACCATTGACACC+AGG - chr5_2:73661798-73661817 None:intergenic 45.0%
! CTGGTGTCAATGGTAGAAGT+TGG + chr5_2:73661796-73661815 Msa0787700:three_prime_UTR 45.0%
CGATCTATATGCTGCGATCT+TGG + chr5_2:73661828-73661847 Msa0787700:three_prime_UTR 45.0%
CAAAAAGTTGCTACAGACGG+AGG - chr5_2:73661939-73661958 None:intergenic 45.0%
! CCGTCTGTAGCAACTTTTTG+TGG + chr5_2:73661939-73661958 Msa0787700:three_prime_UTR 45.0%
CTACCACCACTAAAACAGCA+TGG - chr5_2:73661979-73661998 None:intergenic 45.0%
CTCTGTTTAGGTCTTGACAG+TGG + chr5_2:73662066-73662085 Msa0787700:three_prime_UTR 45.0%
TTTAGGTCTTGACAGTGGAG+TGG + chr5_2:73662071-73662090 Msa0787700:three_prime_UTR 45.0%
!!! AATTATTATTTTCTAATTTT+CGG - chr5_2:73659953-73659972 None:intergenic 5.0%
!! TTAAATAAAAAAAAGTTTAT+GGG + chr5_2:73661757-73661776 Msa0787700:three_prime_UTR 5.0%
ACACGCCGAAGAAAACTGTG+AGG - chr5_2:73659072-73659091 None:intergenic 50.0%
!! GCCTCATGTGGATTTTGGGT+TGG + chr5_2:73660226-73660245 Msa0787700:intron 50.0%
GCCAACCCAAAATCCACATG+AGG - chr5_2:73660230-73660249 None:intergenic 50.0%
!! TTTGGGTTGGCGATATCTGG+AGG + chr5_2:73660239-73660258 Msa0787700:intron 50.0%
!! TTGGGTTGGCGATATCTGGA+GGG + chr5_2:73660240-73660259 Msa0787700:intron 50.0%
ATGTTCCATGGAGAGAGCTG+TGG - chr5_2:73660363-73660382 None:intergenic 50.0%
ACAGCTCTCTCCATGGAACA+TGG + chr5_2:73660362-73660381 Msa0787700:CDS 50.0%
! TAAGGGAACCTCGAGTTGTG+AGG + chr5_2:73660654-73660673 Msa0787700:CDS 50.0%
! AAGGGAACCTCGAGTTGTGA+GGG + chr5_2:73660655-73660674 Msa0787700:CDS 50.0%
AAACCAACCCTCACAACTCG+AGG - chr5_2:73660665-73660684 None:intergenic 50.0%
GCTAGACCCTTTATACAGGC+TGG - chr5_2:73660801-73660820 None:intergenic 50.0%
AAAGGGTCTAGCATCATGGG+AGG + chr5_2:73660809-73660828 Msa0787700:three_prime_UTR 50.0%
! TTTGCATGATTTGGCCTCCG+AGG + chr5_2:73661434-73661453 Msa0787700:three_prime_UTR 50.0%
! GGGAGCATGAATTATGGTCC+TGG + chr5_2:73661777-73661796 Msa0787700:three_prime_UTR 50.0%
TTGTTGCCATGATTGCAGCG+TGG + chr5_2:73661877-73661896 Msa0787700:three_prime_UTR 50.0%
CACTAACCACGCTGCAATCA+TGG - chr5_2:73661886-73661905 None:intergenic 50.0%
TTGCAGCGTGGTTAGTGTGT+TGG + chr5_2:73661889-73661908 Msa0787700:three_prime_UTR 50.0%
AGACGGAGGAAACTTAGGTC+TGG - chr5_2:73661925-73661944 None:intergenic 50.0%
GCTACAGACGGAGGAAACTT+AGG - chr5_2:73661930-73661949 None:intergenic 50.0%
! TTTTTGTGGTCAGGTGCAGC+AGG + chr5_2:73661953-73661972 Msa0787700:three_prime_UTR 50.0%
!!! AGCAGGCCATGCTGTTTTAG+TGG + chr5_2:73661970-73661989 Msa0787700:three_prime_UTR 50.0%
!!! AGGCCATGCTGTTTTAGTGG+TGG + chr5_2:73661973-73661992 Msa0787700:three_prime_UTR 50.0%
!! TGGGTTGGCGATATCTGGAG+GGG + chr5_2:73660241-73660260 Msa0787700:intron 55.0%
TTCCATGGAGAGAGCTGTGG+CGG - chr5_2:73660360-73660379 None:intergenic 55.0%
! GAACCTCGAGTTGTGAGGGT+TGG + chr5_2:73660659-73660678 Msa0787700:CDS 55.0%
! ATGTCTGGTGTAGCGGAGGC+AGG + chr5_2:73659028-73659047 Msa0787700:five_prime_UTR 60.0%
! TGTCTGGTGTAGCGGAGGCA+GGG + chr5_2:73659029-73659048 Msa0787700:five_prime_UTR 60.0%
!! GAGAGAGCTGTGGCGGATTC+CGG - chr5_2:73660353-73660372 None:intergenic 60.0%
ATCCGCCACAGCTCTCTCCA+TGG + chr5_2:73660355-73660374 Msa0787700:CDS 60.0%
! TGTAGCGGAGGCAGGGAGGT+GGG + chr5_2:73659036-73659055 Msa0787700:five_prime_UTR 65.0%
! CTGGTGTAGCGGAGGCAGGG+AGG + chr5_2:73659032-73659051 Msa0787700:five_prime_UTR 70.0%
! GTGTAGCGGAGGCAGGGAGG+TGG + chr5_2:73659035-73659054 Msa0787700:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 73658776 73662096 73658776 ID=Msa0787700;Name=Msa0787700
chr5_2 mRNA 73658776 73662096 73658776 ID=Msa0787700-mRNA-1;Parent=Msa0787700;Name=Msa0787700-mRNA-1;_AED=0.02;_eAED=0.02;_QI=563|0.85|0.75|1|0.42|0.5|8|1306|173
chr5_2 exon 73658776 73658854 73658776 ID=Msa0787700-mRNA-1:exon:22437;Parent=Msa0787700-mRNA-1
chr5_2 exon 73658880 73659262 73658880 ID=Msa0787700-mRNA-1:exon:22438;Parent=Msa0787700-mRNA-1
chr5_2 exon 73659504 73659635 73659504 ID=Msa0787700-mRNA-1:exon:22439;Parent=Msa0787700-mRNA-1
chr5_2 exon 73659811 73659929 73659811 ID=Msa0787700-mRNA-1:exon:22440;Parent=Msa0787700-mRNA-1
chr5_2 exon 73660014 73660115 73660014 ID=Msa0787700-mRNA-1:exon:22441;Parent=Msa0787700-mRNA-1
chr5_2 exon 73660227 73660232 73660227 ID=Msa0787700-mRNA-1:exon:22442;Parent=Msa0787700-mRNA-1
chr5_2 exon 73660302 73660397 73660302 ID=Msa0787700-mRNA-1:exon:22443;Parent=Msa0787700-mRNA-1
chr5_2 exon 73660623 73662096 73660623 ID=Msa0787700-mRNA-1:exon:22444;Parent=Msa0787700-mRNA-1
chr5_2 five_prime_UTR 73658776 73658854 73658776 ID=Msa0787700-mRNA-1:five_prime_utr;Parent=Msa0787700-mRNA-1
chr5_2 five_prime_UTR 73658880 73659262 73658880 ID=Msa0787700-mRNA-1:five_prime_utr;Parent=Msa0787700-mRNA-1
chr5_2 five_prime_UTR 73659504 73659604 73659504 ID=Msa0787700-mRNA-1:five_prime_utr;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73659605 73659635 73659605 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73659811 73659929 73659811 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73660014 73660115 73660014 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73660227 73660232 73660227 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73660302 73660397 73660302 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 CDS 73660623 73660790 73660623 ID=Msa0787700-mRNA-1:cds;Parent=Msa0787700-mRNA-1
chr5_2 three_prime_UTR 73660791 73662096 73660791 ID=Msa0787700-mRNA-1:three_prime_utr;Parent=Msa0787700-mRNA-1
Gene Sequence

>Msa0787700

ATGCAAGGTGCTAAGATCAGCAAAATTTCAGGTATGAAAGTTTTGGAAACAAATGAAAAGGAATTGGTCATGAAACAAGTCATCAAATGGACTGGCAACCCAAACATAGTATTGACCTTGCATGTGTTGTCTATGAAGATCAAAGTGCAGTTGGTAGACTTACAAGTTTTTGCGACGCCGCAGATAAGTTTAAGACCTTTGGTGTCTACACTTCCATGTTTTGCAAAGATTGTGGTGTCTTTGATGGAGAAGCCTCATAAATGCAAATCAATAATAAAAATGTTGCTTAATGAAACAATTGCTCTACCGGAATCCGCCACAGCTCTCTCCATGGAACATGGTTTTGACCTCAAGGATTTCTGCTTTCATGCTGATAAAGAAGTATTAAGGGAACCTCGAGTTGTGAGGGTTGGTTTGATTCAGAACTCTATTGCCCTCCCAACAATTGCGCACTTTATCGAGCAAAAAAAGGCTATCTTTGAGAAAGTAAAGCCCATTCAGAACTCTGTTGTTAACTCATGA
Protein sequence

>Msa0787700

MQGAKISKISGMKVLETNEKELVMKQVIKWTGNPNIVLTLHVLSMKIKVQLVDLQVFATPQISLRPLVSTLPCFAKIVVSLMEKPHKCKSIIKMLLNETIALPESATALSMEHGFDLKDFCFHADKEVLREPRVVRVGLIQNSIALPTIAHFIEQKKAIFEKVKPIQNSVVNS*