Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0070170 | Msa0788950 | 0.807726 | 4.389965e-50 | -8.615850e-47 |
| Msa0788950 | Msa0970320 | 0.810048 | 1.398860e-50 | -8.615850e-47 |
| Msa0788950 | Msa1346750 | 0.823137 | 1.628741e-53 | -8.615850e-47 |
| Msa0160670 | Msa0788950 | 0.829558 | 4.812528e-55 | -8.615850e-47 |
| Msa0473330 | Msa0788950 | 0.801436 | 9.014494e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACTTTCAAAGTTATTA+TGG | 0.201569 | 5_2:-76620982 | Msa0788950:exon |
| GGTTGTGAGTTTGCTTTCTA+TGG | 0.257670 | 5_2:-76620812 | Msa0788950:exon |
| CATATAGTTTATCAAGATCA+TGG | 0.411365 | 5_2:-76620833 | Msa0788950:CDS |
| AGAGGAAGACAAGGAGCTTA+TGG | 0.412214 | 5_2:-76620937 | Msa0788950:CDS |
| TCTTACAGCAGTATATCATA+AGG | 0.449795 | 5_2:-76623550 | Msa0788950:intron |
| GCTGAAGAAATTATCTTCAA+AGG | 0.469330 | 5_2:-76623351 | Msa0788950:intron |
| TAACAAAGAAGCAAGATTCA+AGG | 0.485380 | 5_2:+76623600 | None:intergenic |
| AACTTGGAAGATTTAACACT+TGG | 0.489193 | 5_2:-76623381 | Msa0788950:five_prime_UTR |
| TTCAAAGTTATTATGGAGCA+AGG | 0.504540 | 5_2:-76620975 | Msa0788950:exon |
| GTGGCAGTAGCATGCAAGAA+AGG | 0.532651 | 5_2:-76620908 | Msa0788950:CDS |
| GACAAGGAGCTTATGGAGCA+AGG | 0.578268 | 5_2:-76620930 | Msa0788950:CDS |
| GAGAGAAGAAGAGAGAGCGC+CGG | 0.599081 | 5_2:+76623664 | None:intergenic |
| AGGACTTGCAGAGGAAGACA+AGG | 0.601051 | 5_2:-76620946 | Msa0788950:CDS |
| AGTTCAAAACAAACATACAA+AGG | 0.615786 | 5_2:+76620786 | None:intergenic |
| ATATACTGCTGTAAGACGAA+AGG | 0.626568 | 5_2:+76623557 | None:intergenic |
| ATTATGGAGCAAGGAAGACA+AGG | 0.630410 | 5_2:-76620966 | Msa0788950:CDS |
| AGGCAAGGACTTATTGAGCA+AGG | 0.642985 | 5_2:-76620888 | Msa0788950:CDS |
| AGGAAGACAAGGACTTGCAG+AGG | 0.645505 | 5_2:-76620955 | Msa0788950:CDS |
| AGTAGCATGCAAGAAAGGCA+AGG | 0.663657 | 5_2:-76620903 | Msa0788950:CDS |
| AAGGAGCTTATGGAGCAAGG+TGG | 0.732569 | 5_2:-76620927 | Msa0788950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGAAAAAAAATAAAAATG+AGG | - | chr5_2:76621872-76621891 | Msa0788950:intron | 10.0% |
| !! | TTAAATGATAAAAAAAAGAT+TGG | + | chr5_2:76622046-76622065 | None:intergenic | 10.0% |
| !!! | ATGAAATTTTATGAATTTTT+AGG | - | chr5_2:76622411-76622430 | Msa0788950:intron | 10.0% |
| !!! | AAAAAAAAAAACCTTTTTAA+AGG | + | chr5_2:76622972-76622991 | None:intergenic | 10.0% |
| !! | TATTTAAATTAGATGAAAAA+AGG | - | chr5_2:76623118-76623137 | Msa0788950:intron | 10.0% |
| !! | TAAAAATTGAACAAAAAACA+AGG | + | chr5_2:76621660-76621679 | None:intergenic | 15.0% |
| !! | TTATATATCTTGAAAACAAA+TGG | + | chr5_2:76621744-76621763 | None:intergenic | 15.0% |
| !! | AAAGCATAGTAAAAAAAAAA+TGG | - | chr5_2:76621972-76621991 | Msa0788950:intron | 15.0% |
| !! | TTTAGTGAAAAAAGAAAAAA+AGG | - | chr5_2:76622449-76622468 | Msa0788950:intron | 15.0% |
| !! | TTAGTGAAAAAAGAAAAAAA+GGG | - | chr5_2:76622450-76622469 | Msa0788950:intron | 15.0% |
| !!! | TTAATTTTTTTTTACATGGT+TGG | - | chr5_2:76622739-76622758 | Msa0788950:intron | 15.0% |
| !! | AATTAACCTCAAATTAAATT+TGG | - | chr5_2:76623267-76623286 | Msa0788950:intron | 15.0% |
| !! | ATAATAAGAACAACATTCAA+AGG | + | chr5_2:76621004-76621023 | None:intergenic | 20.0% |
| !! | TTTGTATTTACCATAAACAT+AGG | + | chr5_2:76621467-76621486 | None:intergenic | 20.0% |
| !! | TTTAAAAAGACTTGAAAGTA+TGG | + | chr5_2:76621701-76621720 | None:intergenic | 20.0% |
| !!! | TTTCAGTTAGTTTTTTAACA+AGG | + | chr5_2:76621946-76621965 | None:intergenic | 20.0% |
| !!! | GATAAAAAAAAGATTGGTTT+TGG | + | chr5_2:76622040-76622059 | None:intergenic | 20.0% |
| !!! | ATCATTTAAAACATGTAATG+AGG | - | chr5_2:76622057-76622076 | Msa0788950:intron | 20.0% |
| !! | TAGTGAAAAAAGAAAAAAAG+GGG | - | chr5_2:76622451-76622470 | Msa0788950:intron | 20.0% |
| !! | AAAAAAAGGGGAAAATAATA+AGG | - | chr5_2:76622463-76622482 | Msa0788950:intron | 20.0% |
| !!! | TTTAAAACTGTTATGTTCAT+GGG | - | chr5_2:76622662-76622681 | Msa0788950:intron | 20.0% |
| !!! | CTGATTAAAGAGTATTTTAA+AGG | + | chr5_2:76622868-76622887 | None:intergenic | 20.0% |
| !!! | CTTTAAAATACTCTTTAATC+AGG | - | chr5_2:76622866-76622885 | Msa0788950:intron | 20.0% |
| !!! | TTGTTTTCTATTTGATATTG+TGG | - | chr5_2:76622910-76622929 | Msa0788950:intron | 20.0% |
| !! | GCATTACAAATCATTAATTT+AGG | + | chr5_2:76623186-76623205 | None:intergenic | 20.0% |
| !!! | CTTTTACATGTTTTAAACTT+TGG | - | chr5_2:76623448-76623467 | Msa0788950:intron | 20.0% |
| !! | AAGAACTTTCAAAGTTATTA+TGG | - | chr5_2:76623484-76623503 | Msa0788950:intron | 20.0% |
| !!! | TAATTTTTACGGTTTTCAAG+TGG | + | chr5_2:76620775-76620794 | None:intergenic | 25.0% |
| ! | TTTGAAAGAGTAAAAAACAC+CGG | + | chr5_2:76621034-76621053 | None:intergenic | 25.0% |
| !! | TTTTCTTTTCCAGATTAACT+TGG | - | chr5_2:76621069-76621088 | Msa0788950:intron | 25.0% |
| ! | TTAAATCTTCCAAGTTAATC+TGG | + | chr5_2:76621081-76621100 | None:intergenic | 25.0% |
| !! | AATATATTGATGTTTTCTCC+AGG | - | chr5_2:76621271-76621290 | Msa0788950:intron | 25.0% |
| ! | TAGTTTCAGAATTGAAAACA+AGG | + | chr5_2:76621387-76621406 | None:intergenic | 25.0% |
| !!! | ATTTTGAAAGCCTATGTTTA+TGG | - | chr5_2:76621454-76621473 | Msa0788950:intron | 25.0% |
| !!! | TTTAACTTGCCAAAATAAAG+GGG | + | chr5_2:76621802-76621821 | None:intergenic | 25.0% |
| !!! | GTTTAACTTGCCAAAATAAA+GGG | + | chr5_2:76621803-76621822 | None:intergenic | 25.0% |
| !!! | TGTTTAACTTGCCAAAATAA+AGG | + | chr5_2:76621804-76621823 | None:intergenic | 25.0% |
| !! | TTTTGTATACTACTATAACG+AGG | + | chr5_2:76621913-76621932 | None:intergenic | 25.0% |
| !!! | AAAAAAAGATTGGTTTTGGA+TGG | + | chr5_2:76622036-76622055 | None:intergenic | 25.0% |
| ! | TGAAGAAGAGAAAATGAATA+AGG | - | chr5_2:76622379-76622398 | Msa0788950:intron | 25.0% |
| ! | GAAGAAGAGAAAATGAATAA+GGG | - | chr5_2:76622380-76622399 | Msa0788950:intron | 25.0% |
| ! | AAAATGAAACTGAAATGTGA+TGG | + | chr5_2:76622596-76622615 | None:intergenic | 25.0% |
| !!! | GTGATTAAGGTAGTTTTTTT+TGG | - | chr5_2:76622638-76622657 | Msa0788950:intron | 25.0% |
| !!! | CTTTAAAACTGTTATGTTCA+TGG | - | chr5_2:76622661-76622680 | Msa0788950:intron | 25.0% |
| !!! | TTTTTTTTACATGGTTGGTT+TGG | - | chr5_2:76622744-76622763 | Msa0788950:intron | 25.0% |
| ! | AAGCAGAAAAAAAACAATAG+AGG | + | chr5_2:76622815-76622834 | None:intergenic | 25.0% |
| !! | GATCTATCTATCCTTTAAAA+AGG | - | chr5_2:76622958-76622977 | Msa0788950:intron | 25.0% |
| !! | TGTTCTCTTTTTATCTAAAG+TGG | - | chr5_2:76623038-76623057 | Msa0788950:intron | 25.0% |
| !!! | ACTTTTAAGTTGAGGTTAAA+AGG | + | chr5_2:76623333-76623352 | None:intergenic | 25.0% |
| ! | GTTAACAAGTACTAATAGTT+TGG | - | chr5_2:76623425-76623444 | Msa0788950:intron | 25.0% |
| ! | CATATAGTTTATCAAGATCA+TGG | - | chr5_2:76623633-76623652 | Msa0788950:five_prime_UTR | 25.0% |
| ! | AGTTCAAAACAAACATACAA+AGG | + | chr5_2:76623683-76623702 | None:intergenic | 25.0% |
| TAACAAAGAAGCAAGATTCA+AGG | + | chr5_2:76620869-76620888 | None:intergenic | 30.0% | |
| TCTTACAGCAGTATATCATA+AGG | - | chr5_2:76620916-76620935 | Msa0788950:CDS | 30.0% | |
| !! | TGTTCTTATTATTTCTGCAC+CGG | - | chr5_2:76621012-76621031 | Msa0788950:intron | 30.0% |
| AACTTGGAAGATTTAACACT+TGG | - | chr5_2:76621085-76621104 | Msa0788950:intron | 30.0% | |
| GCTGAAGAAATTATCTTCAA+AGG | - | chr5_2:76621115-76621134 | Msa0788950:intron | 30.0% | |
| TGAGTTAAAACAGAAAATCC+AGG | + | chr5_2:76621213-76621232 | None:intergenic | 30.0% | |
| TAATATCAAAAACAGAAGCC+TGG | + | chr5_2:76621292-76621311 | None:intergenic | 30.0% | |
| !!! | TGACTCATAGCTATCTTTTT+TGG | - | chr5_2:76621578-76621597 | Msa0788950:intron | 30.0% |
| !!! | CAGTTAGTTTTTTAACAAGG+AGG | + | chr5_2:76621943-76621962 | None:intergenic | 30.0% |
| ! | TGAGGAAGATTAATTTTTCG+TGG | - | chr5_2:76622075-76622094 | Msa0788950:intron | 30.0% |
| AATTTCGCAATAGAGAATTG+AGG | - | chr5_2:76622101-76622120 | Msa0788950:intron | 30.0% | |
| !! | AATCTTTAACTTGTGGCATA+TGG | + | chr5_2:76622129-76622148 | None:intergenic | 30.0% |
| TATGCCACAAGTTAAAGATT+TGG | - | chr5_2:76622129-76622148 | Msa0788950:intron | 30.0% | |
| !! | TCTACCAAATCTTTAACTTG+TGG | + | chr5_2:76622136-76622155 | None:intergenic | 30.0% |
| CGATAGATTGACAAACTATA+GGG | + | chr5_2:76622161-76622180 | None:intergenic | 30.0% | |
| ! | CCTATAGTTTGTCAATCTAT+CGG | - | chr5_2:76622159-76622178 | Msa0788950:intron | 30.0% |
| TCAATGATTAAAGAGTTGTC+AGG | - | chr5_2:76622253-76622272 | Msa0788950:intron | 30.0% | |
| CAATGATTAAAGAGTTGTCA+GGG | - | chr5_2:76622254-76622273 | Msa0788950:intron | 30.0% | |
| AACTACCTTAATCACAGATA+TGG | + | chr5_2:76622633-76622652 | None:intergenic | 30.0% | |
| !!! | TGGTTTTGTTTGACACTAAA+AGG | - | chr5_2:76622778-76622797 | Msa0788950:intron | 30.0% |
| CTATTTGATATTGTGGAACT+TGG | - | chr5_2:76622917-76622936 | Msa0788950:intron | 30.0% | |
| GAATCTGTTACTGAATATAG+TGG | + | chr5_2:76623084-76623103 | None:intergenic | 30.0% | |
| ATATGAACTAAGCTTAGTGT+TGG | - | chr5_2:76623160-76623179 | Msa0788950:intron | 30.0% | |
| !! | TCGCAACCAAATTTAATTTG+AGG | + | chr5_2:76623276-76623295 | None:intergenic | 30.0% |
| !!! | GCATCATTACTTTTAAGTTG+AGG | + | chr5_2:76623341-76623360 | None:intergenic | 30.0% |
| TTCAAAGTTATTATGGAGCA+AGG | - | chr5_2:76623491-76623510 | Msa0788950:intron | 30.0% | |
| ATATACTGCTGTAAGACGAA+AGG | + | chr5_2:76620912-76620931 | None:intergenic | 35.0% | |
| TGTGATTCTTAGTTGAATCC+TGG | - | chr5_2:76621192-76621211 | Msa0788950:intron | 35.0% | |
| !! | AAGTCCAATCCCCTTTATTT+TGG | - | chr5_2:76621790-76621809 | Msa0788950:intron | 35.0% |
| TGTATACTACTATAACGAGG+AGG | + | chr5_2:76621910-76621929 | None:intergenic | 35.0% | |
| TGTAGTGCATTCCTTGAAAA+AGG | - | chr5_2:76621995-76622014 | Msa0788950:intron | 35.0% | |
| GTAGTGCATTCCTTGAAAAA+GGG | - | chr5_2:76621996-76622015 | Msa0788950:intron | 35.0% | |
| !! | TGTAGTTGTACCCTTTTTCA+AGG | + | chr5_2:76622009-76622028 | None:intergenic | 35.0% |
| CCGATAGATTGACAAACTAT+AGG | + | chr5_2:76622162-76622181 | None:intergenic | 35.0% | |
| GAGTTTCTCGTATATAGAGA+TGG | + | chr5_2:76622196-76622215 | None:intergenic | 35.0% | |
| CGAATTGATGAGACATTGAT+TGG | + | chr5_2:76622329-76622348 | None:intergenic | 35.0% | |
| ATGAAACTGAAATGTGATGG+TGG | + | chr5_2:76622593-76622612 | None:intergenic | 35.0% | |
| CATCTCCATATCTGTGATTA+AGG | - | chr5_2:76622625-76622644 | Msa0788950:intron | 35.0% | |
| TGATATTGTGGAACTTGGAT+CGG | - | chr5_2:76622922-76622941 | Msa0788950:intron | 35.0% | |
| CACGTTTCATTGTATGAAGA+TGG | + | chr5_2:76623376-76623395 | None:intergenic | 35.0% | |
| ! | CCGGCTGAGAGTAATTTTTA+CGG | + | chr5_2:76620786-76620805 | None:intergenic | 40.0% |
| CCGTAAAAATTACTCTCAGC+CGG | - | chr5_2:76620783-76620802 | Msa0788950:three_prime_UTR | 40.0% | |
| CTTGCCAAAATAAAGGGGAT+TGG | + | chr5_2:76621797-76621816 | None:intergenic | 40.0% | |
| TGGCAAGTTAAACACCATCA+AGG | - | chr5_2:76621810-76621829 | Msa0788950:intron | 40.0% | |
| GGCAAGTTAAACACCATCAA+GGG | - | chr5_2:76621811-76621830 | Msa0788950:intron | 40.0% | |
| GGGATTAAAGAATCCCTTGA+TGG | + | chr5_2:76621827-76621846 | None:intergenic | 40.0% | |
| CCACTATGTCGTTGTTCTTA+GGG | + | chr5_2:76621847-76621866 | None:intergenic | 40.0% | |
| ACCACTATGTCGTTGTTCTT+AGG | + | chr5_2:76621848-76621867 | None:intergenic | 40.0% | |
| ! | CTTTCGTGTAGCATGGATTT+TGG | + | chr5_2:76622218-76622237 | None:intergenic | 40.0% |
| TGTTGATCTTTCGTGTAGCA+TGG | + | chr5_2:76622225-76622244 | None:intergenic | 40.0% | |
| GATTGGAGCGTGAATCTTTA+TGG | + | chr5_2:76622312-76622331 | None:intergenic | 40.0% | |
| !! | TACATGGTTGGTTTGGTTCT+TGG | - | chr5_2:76622751-76622770 | Msa0788950:intron | 40.0% |
| !! | TTGGTTTGGTTCTTGGATCT+TGG | - | chr5_2:76622758-76622777 | Msa0788950:intron | 40.0% |
| TTGGATCGGCTGTCAAAAAA+AGG | - | chr5_2:76622936-76622955 | Msa0788950:intron | 40.0% | |
| ACTCAAGAGCTAGTTACTTC+TGG | - | chr5_2:76623302-76623321 | Msa0788950:intron | 40.0% | |
| ATTATGGAGCAAGGAAGACA+AGG | - | chr5_2:76623500-76623519 | Msa0788950:intron | 40.0% | |
| ! | GGTTGTGAGTTTGCTTTCTA+TGG | - | chr5_2:76623654-76623673 | Msa0788950:five_prime_UTR | 40.0% |
| GGGGATTGGACTTGACAAAT+CGG | + | chr5_2:76621783-76621802 | None:intergenic | 45.0% | |
| CCCTAAGAACAACGACATAG+TGG | - | chr5_2:76621844-76621863 | Msa0788950:intron | 45.0% | |
| AGAGGAAGACAAGGAGCTTA+TGG | - | chr5_2:76623529-76623548 | Msa0788950:intron | 45.0% | |
| AGTAGCATGCAAGAAAGGCA+AGG | - | chr5_2:76623563-76623582 | Msa0788950:five_prime_UTR | 45.0% | |
| AGGCAAGGACTTATTGAGCA+AGG | - | chr5_2:76623578-76623597 | Msa0788950:five_prime_UTR | 45.0% | |
| !!! | TACTTTAATTTTTTTTTTTT+AGG | - | chr5_2:76620944-76620963 | Msa0788950:CDS | 5.0% |
| !!! | ACTTTAATTTTTTTTTTTTA+GGG | - | chr5_2:76620945-76620964 | Msa0788950:CDS | 5.0% |
| !!! | ATTTTTAATTTTTTTTTACA+TGG | - | chr5_2:76622735-76622754 | Msa0788950:intron | 5.0% |
| !! | ATGTAATTTAAAAAATATAA+GGG | + | chr5_2:76623208-76623227 | None:intergenic | 5.0% |
| !! | AATGTAATTTAAAAAATATA+AGG | + | chr5_2:76623209-76623228 | None:intergenic | 5.0% |
| !!! | AGTTTTTTTTTTTTTTTTTA+AGG | - | chr5_2:76623231-76623250 | Msa0788950:intron | 5.0% |
| AGGAAGACAAGGACTTGCAG+AGG | - | chr5_2:76623511-76623530 | Msa0788950:intron | 50.0% | |
| AGGACTTGCAGAGGAAGACA+AGG | - | chr5_2:76623520-76623539 | Msa0788950:intron | 50.0% | |
| GACAAGGAGCTTATGGAGCA+AGG | - | chr5_2:76623536-76623555 | Msa0788950:intron | 50.0% | |
| AAGGAGCTTATGGAGCAAGG+TGG | - | chr5_2:76623539-76623558 | Msa0788950:intron | 50.0% | |
| GTGGCAGTAGCATGCAAGAA+AGG | - | chr5_2:76623558-76623577 | Msa0788950:five_prime_UTR | 50.0% | |
| ! | GAGAGAAGAAGAGAGAGCGC+CGG | + | chr5_2:76620805-76620824 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 76620754 | 76623734 | 76620754 | ID=Msa0788950;Name=Msa0788950 |
| chr5_2 | mRNA | 76620754 | 76623734 | 76620754 | ID=Msa0788950-mRNA-1;Parent=Msa0788950;Name=Msa0788950-mRNA-1;_AED=0.59;_eAED=0.59;_QI=255|0.5|0.66|1|0|0.33|3|73|52 |
| chr5_2 | exon | 76620754 | 76621001 | 76620754 | ID=Msa0788950-mRNA-1:exon:23334;Parent=Msa0788950-mRNA-1 |
| chr5_2 | exon | 76623352 | 76623406 | 76623352 | ID=Msa0788950-mRNA-1:exon:23333;Parent=Msa0788950-mRNA-1 |
| chr5_2 | exon | 76623551 | 76623734 | 76623551 | ID=Msa0788950-mRNA-1:exon:23332;Parent=Msa0788950-mRNA-1 |
| chr5_2 | five_prime_UTR | 76623551 | 76623734 | 76623551 | ID=Msa0788950-mRNA-1:five_prime_utr;Parent=Msa0788950-mRNA-1 |
| chr5_2 | five_prime_UTR | 76623352 | 76623406 | 76623352 | ID=Msa0788950-mRNA-1:five_prime_utr;Parent=Msa0788950-mRNA-1 |
| chr5_2 | five_prime_UTR | 76620986 | 76621001 | 76620986 | ID=Msa0788950-mRNA-1:five_prime_utr;Parent=Msa0788950-mRNA-1 |
| chr5_2 | CDS | 76620827 | 76620985 | 76620827 | ID=Msa0788950-mRNA-1:cds;Parent=Msa0788950-mRNA-1 |
| chr5_2 | three_prime_UTR | 76620754 | 76620826 | 76620754 | ID=Msa0788950-mRNA-1:three_prime_utr;Parent=Msa0788950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
