AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0803040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0803040 MtrunA17_Chr2g0282761 98.182 55 1 0 1 55 1 55 3.14e-33 115
Msa0803040 MtrunA17_Chr4g0040501 83.636 55 9 0 1 55 1 55 5.20e-27 99.8
Msa0803040 MtrunA17_Chr2g0282741 81.818 55 10 0 1 55 1 55 2.63e-26 97.8
Msa0803040 MtrunA17_Chr4g0006391 74.545 55 14 0 1 55 1 55 1.42e-25 95.9
Msa0803040 MtrunA17_Chr3g0138471 77.778 54 12 0 1 54 1 54 3.91e-25 94.4
Msa0803040 MtrunA17_Chr2g0324921 74.545 55 14 0 1 55 1 55 2.97e-24 92.4
Msa0803040 MtrunA17_Chr7g0263971 55.769 52 23 0 4 55 369 420 1.02e-15 69.3
Msa0803040 MtrunA17_Chr7g0269841 60.784 51 19 1 5 55 27 76 3.46e-15 67.8
Msa0803040 MtrunA17_Chr5g0415731 50.847 59 22 1 4 55 5 63 1.86e-12 58.2
Msa0803040 MtrunA17_Chr3g0123861 57.692 52 20 1 4 55 82 131 5.61e-12 58.9
Msa0803040 MtrunA17_Chr5g0427221 51.923 52 24 1 4 55 48 98 7.10e-12 56.6
Msa0803040 MtrunA17_Chr7g0271891 45.283 53 26 1 5 54 17 69 8.58e-12 56.6
Msa0803040 MtrunA17_Chr5g0426951 45.000 60 32 1 4 63 83 141 1.56e-11 57.4
Msa0803040 MtrunA17_Chr3g0093111 48.148 54 27 1 3 55 1 54 2.01e-11 57.0
Msa0803040 MtrunA17_Chr4g0021141 49.020 51 25 1 4 54 22 71 2.78e-11 54.7
Msa0803040 MtrunA17_Chr2g0307061 46.032 63 30 2 4 64 110 170 2.79e-11 56.6
Msa0803040 MtrunA17_Chr3g0091361 47.170 53 28 0 3 55 4 56 5.17e-11 55.1
Msa0803040 MtrunA17_Chr3g0087741 50.000 52 25 1 4 55 89 139 5.48e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0803040 AT5G01390.3 74.545 55 14 0 1 55 1 55 1.65e-25 94.0
Msa0803040 AT5G01390.4 74.545 55 14 0 1 55 1 55 2.40e-25 93.6
Msa0803040 AT5G01390.2 74.545 55 14 0 1 55 1 55 3.88e-25 94.0
Msa0803040 AT4G28480.2 74.545 55 14 0 1 55 1 55 7.30e-25 93.6
Msa0803040 AT3G08910.1 76.364 55 13 0 1 55 1 55 9.69e-25 93.6
Msa0803040 AT2G20560.1 74.545 55 14 0 1 55 1 55 9.78e-25 94.0
Msa0803040 AT4G28480.1 74.545 55 14 0 1 55 1 55 1.42e-24 93.6
Msa0803040 AT5G25530.1 74.545 55 14 0 1 55 1 55 1.68e-24 93.6
Msa0803040 AT5G01390.1 74.545 55 14 0 1 55 1 55 3.22e-24 92.4
Msa0803040 AT5G25530.2 74.545 55 14 0 1 55 1 55 5.73e-24 92.0
Msa0803040 AT1G10350.1 57.143 63 27 0 1 63 1 63 3.99e-21 84.3
Msa0803040 AT1G59725.1 64.286 56 19 1 1 55 1 56 1.48e-19 80.1
Msa0803040 AT3G47940.1 66.071 56 18 1 1 55 1 56 2.02e-19 79.7
Msa0803040 AT5G03160.1 51.923 52 25 0 4 55 370 421 1.19e-13 63.9
Msa0803040 AT2G41000.1 45.614 57 27 1 3 55 2 58 3.45e-13 60.8
Msa0803040 AT2G41000.2 45.614 57 27 1 3 55 2 58 3.99e-13 61.2
Msa0803040 AT2G22360.1 59.615 52 19 1 4 55 86 135 1.33e-12 60.8
Msa0803040 AT4G39960.1 57.692 52 20 1 4 55 85 134 3.06e-12 60.1
Msa0803040 AT4G39960.2 57.692 52 20 1 4 55 85 134 3.06e-12 60.1
Msa0803040 AT3G62600.1 51.852 54 25 1 2 55 24 76 3.30e-12 59.7
Msa0803040 AT5G48030.1 55.769 52 22 1 4 55 94 144 3.95e-12 59.7
Msa0803040 AT1G56300.2 48.148 54 25 1 5 55 14 67 8.59e-12 56.2
Msa0803040 AT1G56300.1 48.148 54 25 1 5 55 14 67 1.06e-11 56.6
Msa0803040 AT5G16650.1 54.902 51 22 1 4 54 11 60 2.91e-11 55.1
Msa0803040 AT5G16650.2 54.902 51 22 1 4 54 11 60 4.03e-11 55.5
Msa0803040 AT3G17830.1 55.357 56 19 2 2 55 61 112 4.54e-11 56.6
Msa0803040 AT1G59980.2 50.000 50 24 1 6 55 25 73 5.29e-11 56.2
Msa0803040 AT1G68370.1 52.941 51 23 1 4 54 17 66 9.42e-11 55.8

Find 32 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGATTACTATGAGATTT+TGG 0.205034 5_3:-18005103 Msa0803040:CDS
GTAGATTTGTTGTGCGTAAT+TGG 0.247560 5_3:-18005918 Msa0803040:five_prime_UTR
TTATTGTCAGGATTCTTATC+AGG 0.253732 5_3:+18005012 None:intergenic
ACAGATTAAACCTTTATTCT+TGG 0.256184 5_3:+18005945 None:intergenic
CTTATCAGGGTGCCATTTCA+TGG 0.338061 5_3:+18005026 None:intergenic
ATCAACAAATGAAGAGGAAA+TGG 0.344798 5_3:-18002831 Msa0803040:CDS
GATTCCATGACCAAGAATAA+AGG 0.364161 5_3:-18005955 Msa0803040:five_prime_UTR
ATAGAAAACTTGCCATGAAA+TGG 0.412382 5_3:-18005038 Msa0803040:CDS
TGGTTCTAGCCTGGTGGATA+AGG 0.413461 5_3:-18005788 Msa0803040:five_prime_UTR
TAAACCTTTATTCTTGGTCA+TGG 0.433411 5_3:+18005951 None:intergenic
TCAGCATCATTCTTATTGTC+AGG 0.435149 5_3:+18005000 None:intergenic
GACTAAATTCAAACTCATAT+CGG 0.437957 5_3:-18004977 Msa0803040:CDS
TCAACAAATGAAGAGGAAAT+GGG 0.460550 5_3:-18002830 Msa0803040:CDS
GAAAGCAAAGTAGTGGAAGA+TGG 0.462215 5_3:-18005127 Msa0803040:exon
TATTGTCAGGATTCTTATCA+GGG 0.462954 5_3:+18005013 None:intergenic
AAAGTAGTGGAAGATGGGTT+TGG 0.472595 5_3:-18005121 Msa0803040:exon
ACTCATATCGGAAGCATATG+AGG 0.475266 5_3:-18004965 Msa0803040:intron
GAAAAGTGAAAGCAAAGTAG+TGG 0.495138 5_3:-18005134 Msa0803040:five_prime_UTR
AAATGAAGAGGAAATGGGGC+AGG 0.501521 5_3:-18002825 Msa0803040:CDS
AAAGCAAAGTAGTGGAAGAT+GGG 0.505371 5_3:-18005126 Msa0803040:exon
GGTGGATAAGGCAAACATAC+AGG 0.505654 5_3:-18005776 Msa0803040:intron
GATTTGTTGTGCGTAATTGG+AGG 0.553928 5_3:-18005915 Msa0803040:five_prime_UTR
GTATGTTTGCCTTATCCACC+AGG 0.559232 5_3:+18005779 None:intergenic
AGAGACATCAACAAATGAAG+AGG 0.574174 5_3:-18002837 Msa0803040:CDS
AAAGAAAGTCCTATCTTGAG+TGG 0.579591 5_3:-18005161 Msa0803040:five_prime_UTR
TATCCACCAGGCTAGAACCA+TGG 0.582402 5_3:+18005791 None:intergenic
AATGTGTGGACTTTCTCTGA+TGG 0.591588 5_3:-18005983 Msa0803040:five_prime_UTR
TTTCCATGGTTCTAGCCTGG+TGG 0.591889 5_3:-18005794 Msa0803040:five_prime_UTR
AGAGTGTCTACAAACAGAGC+AGG 0.623091 5_3:+18005876 None:intergenic
CAACAAATGAAGAGGAAATG+GGG 0.649949 5_3:-18002829 Msa0803040:CDS
GACTGAAATGTATTAATGTG+TGG 0.658305 5_3:-18005997 Msa0803040:five_prime_UTR
AATGAAGAGGAAATGGGGCA+GGG 0.662272 5_3:-18002824 Msa0803040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTGATTACTATTTATA+TGG - chr5_3:18003253-18003272 Msa0803040:intron 15.0%
!!! AATTTTTTTCCTTCATTATT+AGG - chr5_3:18004216-18004235 Msa0803040:intron 15.0%
!!! ATTTTTTTCCTTCATTATTA+GGG - chr5_3:18004217-18004236 Msa0803040:intron 15.0%
!! TTATATATGAAAAGCTTAAT+AGG + chr5_3:18004414-18004433 None:intergenic 15.0%
!! AACTTCAAAAATATCAATAA+TGG + chr5_3:18004445-18004464 None:intergenic 15.0%
!!! CATAAAATAGACTTTTAAAT+GGG - chr5_3:18004638-18004657 Msa0803040:intron 15.0%
!!! TTGATGTATTTTAATTTAGT+GGG + chr5_3:18005463-18005482 None:intergenic 15.0%
!!! ATTGATGTATTTTAATTTAG+TGG + chr5_3:18005464-18005483 None:intergenic 15.0%
!! AAAAATAACCATCATGTAAT+GGG + chr5_3:18003126-18003145 Msa0803030:three_prime_UTR 20.0%
!! TAAAAATAACCATCATGTAA+TGG + chr5_3:18003127-18003146 Msa0803030:three_prime_UTR 20.0%
!! TGCAAAATTAACATTTAAGT+TGG - chr5_3:18003221-18003240 Msa0803040:intron 20.0%
!!! TCTATTATTGTGTTGTTTAA+CGG - chr5_3:18003297-18003316 Msa0803040:intron 20.0%
!! TTTATCTGTTACAACAAAAT+TGG - chr5_3:18003360-18003379 Msa0803040:intron 20.0%
!! TGTAATGATTATTATTGAAC+AGG + chr5_3:18003636-18003655 None:intergenic 20.0%
!!! AAATTGAAAATTGAGGATTT+TGG + chr5_3:18004201-18004220 None:intergenic 20.0%
!! AGGAAAAAAATTGAAAATTG+AGG + chr5_3:18004208-18004227 None:intergenic 20.0%
!!! TTTTTTTCCTTCATTATTAG+GGG - chr5_3:18004218-18004237 Msa0803040:intron 20.0%
!!! TAAAAGTCTATTTTATGCTA+AGG + chr5_3:18004635-18004654 None:intergenic 20.0%
!!! GCATAAAATAGACTTTTAAA+TGG - chr5_3:18004637-18004656 Msa0803040:intron 20.0%
!!! TTTTTATTTTGGTAAAACCA+AGG + chr5_3:18005046-18005065 None:intergenic 20.0%
!!! ATTGAAATTTTACTGAATGA+TGG + chr5_3:18005153-18005172 None:intergenic 20.0%
!!! TGATGTATTTTAATTTAGTG+GGG + chr5_3:18005462-18005481 None:intergenic 20.0%
! ACAGATTAAACCTTTATTCT+TGG + chr5_3:18002906-18002925 Msa0803030:intron 25.0%
! AATGTGGTATACTTAAAGTT+TGG + chr5_3:18003191-18003210 Msa0803030:three_prime_UTR 25.0%
! ATTCTCACTATACTTTAACA+AGG - chr5_3:18003323-18003342 Msa0803040:intron 25.0%
! ATTATTATTGAACAGGACTA+TGG + chr5_3:18003629-18003648 None:intergenic 25.0%
!! TTTGGATTACTATGAGATTT+TGG - chr5_3:18003745-18003764 Msa0803040:intron 25.0%
! GACTAAATTCAAACTCATAT+CGG - chr5_3:18003871-18003890 Msa0803040:intron 25.0%
! GTTTAATTGCTACAAAACTA+TGG - chr5_3:18003936-18003955 Msa0803040:intron 25.0%
!!! ATTATCTTGTTTCTCTGTTT+TGG - chr5_3:18004006-18004025 Msa0803040:intron 25.0%
!!! AAAATTGAGGATTTTGGTAA+GGG + chr5_3:18004195-18004214 None:intergenic 25.0%
! TAAGGCCTATTTAATTAAAC+AGG - chr5_3:18004343-18004362 Msa0803040:intron 25.0%
! ATATGAAAAGCTTAATAGGT+CGG + chr5_3:18004410-18004429 None:intergenic 25.0%
! CTAAATAACCAATCATATGA+AGG + chr5_3:18004487-18004506 None:intergenic 25.0%
! AAATAGGCCAAAACTTATTA+AGG + chr5_3:18004585-18004604 None:intergenic 25.0%
!!! TAATAAGTTTTGGCCTATTT+AGG - chr5_3:18004585-18004604 Msa0803040:intron 25.0%
! TTCAGATTGGATAATGAATA+GGG + chr5_3:18004813-18004832 None:intergenic 25.0%
! ATTCAGATTGGATAATGAAT+AGG + chr5_3:18004814-18004833 None:intergenic 25.0%
!! ATATGTTTTTCCATATACCA+CGG + chr5_3:18004948-18004967 None:intergenic 25.0%
! GGAAAAACATATCTTATGAT+AGG - chr5_3:18004956-18004975 Msa0803040:intron 25.0%
! GTAACTAAGATCAATTTAAG+AGG + chr5_3:18004986-18005005 None:intergenic 25.0%
! ATTGATCTTAGTTACTTCAA+AGG - chr5_3:18004991-18005010 Msa0803040:CDS 25.0%
!!! GCACACAATTCTTTTTATTT+TGG + chr5_3:18005057-18005076 None:intergenic 25.0%
!! ACTTGTTGAGTAATGTTATA+AGG - chr5_3:18005100-18005119 Msa0803040:CDS 25.0%
! CTTGTTGAGTAATGTTATAA+GGG - chr5_3:18005101-18005120 Msa0803040:CDS 25.0%
! TATCCTGTCAAAAAATAAAC+AGG - chr5_3:18005385-18005404 Msa0803040:intron 25.0%
!!! TAACCTGTTTATTTTTTGAC+AGG + chr5_3:18005391-18005410 None:intergenic 25.0%
! ATGATACTGCGTAAATTAAA+AGG - chr5_3:18005533-18005552 Msa0803040:intron 25.0%
! GTTTAATTATCCAGTGATTA+TGG + chr5_3:18005709-18005728 None:intergenic 25.0%
!!! CAATTCTTTGAAATTTCTGT+GGG - chr5_3:18005790-18005809 Msa0803040:five_prime_UTR 25.0%
! AAGCAGAATAGTAGTATAAT+AGG + chr5_3:18005986-18006005 None:intergenic 25.0%
GACTGAAATGTATTAATGTG+TGG - chr5_3:18002851-18002870 Msa0803040:intron 30.0%
TAAACCTTTATTCTTGGTCA+TGG + chr5_3:18002900-18002919 Msa0803030:intron 30.0%
TTACAGTTTCAAAAAAGGTC+GGG + chr5_3:18003007-18003026 Msa0803030:intron 30.0%
GTTACAGTTTCAAAAAAGGT+CGG + chr5_3:18003008-18003027 Msa0803030:intron 30.0%
CATCGTTACAGTTTCAAAAA+AGG + chr5_3:18003012-18003031 Msa0803030:intron 30.0%
! TGTTGCAAAAAGATTTTCCA+TGG - chr5_3:18003040-18003059 Msa0803040:intron 30.0%
TTAAAGTTTGGCCTAATTAC+TGG + chr5_3:18003179-18003198 Msa0803030:three_prime_UTR 30.0%
TTTGCACTATGAAAAGAATG+TGG + chr5_3:18003207-18003226 Msa0803030:three_prime_UTR 30.0%
TTCAAAATTCCTGCTAGATA+GGG - chr5_3:18003388-18003407 Msa0803040:intron 30.0%
! TGATGCTGAAATTTTCTGTT+GGG - chr5_3:18003489-18003508 Msa0803040:intron 30.0%
ATAGAAAACTTGCCATGAAA+TGG - chr5_3:18003810-18003829 Msa0803040:intron 30.0%
TATTGTCAGGATTCTTATCA+GGG + chr5_3:18003838-18003857 None:intergenic 30.0%
TTATTGTCAGGATTCTTATC+AGG + chr5_3:18003839-18003858 None:intergenic 30.0%
!! TGTTTACTGTCTTTTGTTTG+AGG - chr5_3:18004052-18004071 Msa0803040:intron 30.0%
TACAATCAGTGATATACAGT+CGG - chr5_3:18004110-18004129 Msa0803040:intron 30.0%
AAATTCTTTGACCCTTCTTA+GGG + chr5_3:18004152-18004171 None:intergenic 30.0%
AAAATTCTTTGACCCTTCTT+AGG + chr5_3:18004153-18004172 None:intergenic 30.0%
!! GAAAATTGAGGATTTTGGTA+AGG + chr5_3:18004196-18004215 None:intergenic 30.0%
CAAACATATTTGTGTGCTTT+AGG - chr5_3:18004559-18004578 Msa0803040:intron 30.0%
! CTTTAGGCCTTAATAAGTTT+TGG - chr5_3:18004575-18004594 Msa0803040:intron 30.0%
!!! AGGCCTATAATTTTTGAAGT+AGG - chr5_3:18004740-18004759 Msa0803040:intron 30.0%
TGGCCTACTTCAAAAATTAT+AGG + chr5_3:18004746-18004765 None:intergenic 30.0%
!!! TATAATTTTTGAAGTAGGCC+AGG - chr5_3:18004745-18004764 Msa0803040:intron 30.0%
TCAGATTGGATAATGAATAG+GGG + chr5_3:18004812-18004831 None:intergenic 30.0%
TTCATTATCCAATCTGAATG+TGG - chr5_3:18004815-18004834 Msa0803040:intron 30.0%
! TTTGACTTTCATCCTAAGTA+AGG - chr5_3:18004881-18004900 Msa0803040:intron 30.0%
GATGGAAAATAAGTACAACA+AGG + chr5_3:18005135-18005154 None:intergenic 30.0%
AACATAGATAGACAAAGAGT+TGG + chr5_3:18005202-18005221 None:intergenic 30.0%
TCTTTGTCTATCTATGTTGT+GGG - chr5_3:18005204-18005223 Msa0803040:intron 30.0%
!! TCTGACTTGGTATATTGATT+TGG - chr5_3:18005263-18005282 Msa0803040:intron 30.0%
AGAGCAATTTATGATCAGTA+TGG - chr5_3:18005317-18005336 Msa0803040:intron 30.0%
ATACTCGGATATTTATAGAC+AGG - chr5_3:18005357-18005376 Msa0803040:intron 30.0%
!! TATCATTGCTTCAGTTTTGA+GGG + chr5_3:18005519-18005538 None:intergenic 30.0%
TTCTGCAGATAAGAGTTATT+AGG + chr5_3:18005681-18005700 None:intergenic 30.0%
ATCACTGGATAATTAAACAG+AGG - chr5_3:18005711-18005730 Msa0803040:intron 30.0%
AGAGTTCTATTCCAACTATT+TGG - chr5_3:18005753-18005772 Msa0803040:intron 30.0%
GAGTTCTATTCCAACTATTT+GGG - chr5_3:18005754-18005773 Msa0803040:intron 30.0%
CCACAGAAATTTCAAAGAAT+TGG + chr5_3:18005792-18005811 None:intergenic 30.0%
!! CCAATTCTTTGAAATTTCTG+TGG - chr5_3:18005789-18005808 Msa0803040:five_prime_UTR 30.0%
!!! AGTATTGCTCTTTTGAAGTA+CGG + chr5_3:18005957-18005976 None:intergenic 30.0%
ATCAACAAATGAAGAGGAAA+TGG - chr5_3:18006017-18006036 Msa0803040:five_prime_UTR 30.0%
TCAACAAATGAAGAGGAAAT+GGG - chr5_3:18006018-18006037 Msa0803040:five_prime_UTR 30.0%
GATTCCATGACCAAGAATAA+AGG - chr5_3:18002893-18002912 Msa0803040:intron 35.0%
!! GTAGATTTGTTGTGCGTAAT+TGG - chr5_3:18002930-18002949 Msa0803040:intron 35.0%
! AATGGGTTTGATCAAAACAG+AGG + chr5_3:18003109-18003128 Msa0803030:three_prime_UTR 35.0%
TGATCAAACCCATTACATGA+TGG - chr5_3:18003115-18003134 Msa0803040:intron 35.0%
CTGTTACAACAAAATTGGAG+AGG - chr5_3:18003365-18003384 Msa0803040:intron 35.0%
GTTCAAAATTCCTGCTAGAT+AGG - chr5_3:18003387-18003406 Msa0803040:intron 35.0%
ACATATTTACCCTATCTAGC+AGG + chr5_3:18003400-18003419 Msa0803030:intron 35.0%
! CTGATGCTGAAATTTTCTGT+TGG - chr5_3:18003488-18003507 Msa0803040:intron 35.0%
! GATGCTGAAATTTTCTGTTG+GGG - chr5_3:18003490-18003509 Msa0803040:intron 35.0%
GTTGATCAGAAACTTCACTT+TGG + chr5_3:18003592-18003611 None:intergenic 35.0%
!!! CAGGTTTTTTGTCTTGTTTC+TGG + chr5_3:18003663-18003682 None:intergenic 35.0%
! GATAGGACTTTCTTTTCTTC+AGG + chr5_3:18003682-18003701 None:intergenic 35.0%
AAAGAAAGTCCTATCTTGAG+TGG - chr5_3:18003687-18003706 Msa0803040:intron 35.0%
! TTTTCTCTTCCACTCAAGAT+AGG + chr5_3:18003699-18003718 None:intergenic 35.0%
GAAAAGTGAAAGCAAAGTAG+TGG - chr5_3:18003714-18003733 Msa0803040:intron 35.0%
! AAAGCAAAGTAGTGGAAGAT+GGG - chr5_3:18003722-18003741 Msa0803040:intron 35.0%
! GGATTACTATGAGATTTTGG+AGG - chr5_3:18003748-18003767 Msa0803040:intron 35.0%
TCAGCATCATTCTTATTGTC+AGG + chr5_3:18003851-18003870 None:intergenic 35.0%
! ACTGATCATTCATGTTTCTG+AGG - chr5_3:18003962-18003981 Msa0803040:intron 35.0%
! GTGTTTGAGATCCAATTTGA+TGG - chr5_3:18004086-18004105 Msa0803040:intron 35.0%
ACTGATTGTAGCCATCAAAT+TGG + chr5_3:18004100-18004119 None:intergenic 35.0%
AATTCTTTGACCCTTCTTAG+GGG + chr5_3:18004151-18004170 None:intergenic 35.0%
! TTTTCCTTCATTATTAGGGG+TGG - chr5_3:18004221-18004240 Msa0803040:intron 35.0%
TTTCCTTCATTATTAGGGGT+GGG - chr5_3:18004222-18004241 Msa0803040:intron 35.0%
CCTGGCCTGTTTAATTAAAT+AGG + chr5_3:18004351-18004370 None:intergenic 35.0%
CCTATTTAATTAAACAGGCC+AGG - chr5_3:18004348-18004367 Msa0803040:intron 35.0%
TAATTAAACAGGCCAGGTTA+AGG - chr5_3:18004354-18004373 Msa0803040:intron 35.0%
AACACAAGCCTTCATATGAT+TGG - chr5_3:18004476-18004495 Msa0803040:intron 35.0%
ATGTAAGCCTACTCCTAAAT+AGG + chr5_3:18004601-18004620 None:intergenic 35.0%
! GTCTATTTTATGCTAAGGCT+TGG + chr5_3:18004630-18004649 None:intergenic 35.0%
!! AGACTTTTAAATGGGCTTTC+AGG - chr5_3:18004646-18004665 Msa0803040:intron 35.0%
AAAAATTATAGGCCTGAGCT+TGG + chr5_3:18004735-18004754 None:intergenic 35.0%
TAATGAATAGGGGTGGAAAT+GGG + chr5_3:18004802-18004821 None:intergenic 35.0%
ATAATGAATAGGGGTGGAAA+TGG + chr5_3:18004803-18004822 None:intergenic 35.0%
TTACATGGAACTCCTTACTT+AGG + chr5_3:18004896-18004915 None:intergenic 35.0%
CTCTTTGTCTATCTATGTTG+TGG - chr5_3:18005203-18005222 Msa0803040:intron 35.0%
CTTTGTCTATCTATGTTGTG+GGG - chr5_3:18005205-18005224 Msa0803040:intron 35.0%
CAGATTCTGTGAACATTTCA+TGG + chr5_3:18005247-18005266 None:intergenic 35.0%
AATGTTCACAGAATCTGACT+TGG - chr5_3:18005250-18005269 Msa0803040:intron 35.0%
!! ATATTGATTTGGCTTCATCG+AGG - chr5_3:18005274-18005293 Msa0803040:intron 35.0%
TCATAAATTGCTCTCTCTTG+CGG + chr5_3:18005311-18005330 None:intergenic 35.0%
AGAGCAATTCATACGATACT+CGG - chr5_3:18005342-18005361 Msa0803040:intron 35.0%
ACTCACAGTCTAACTTATCT+TGG - chr5_3:18005426-18005445 Msa0803040:intron 35.0%
! GTATCATTGCTTCAGTTTTG+AGG + chr5_3:18005520-18005539 None:intergenic 35.0%
TTTCGCGTGATTTATAGTTG+TGG + chr5_3:18005634-18005653 None:intergenic 35.0%
!! TGCATTGATGGAAAGCATTT+CGG + chr5_3:18005657-18005676 None:intergenic 35.0%
TGGATAATTAAACAGAGGCA+GGG - chr5_3:18005716-18005735 Msa0803040:intron 35.0%
TTCTATTCCAACTATTTGGG+TGG - chr5_3:18005757-18005776 Msa0803040:intron 35.0%
! TTTTACTCCACCCAAATAGT+TGG + chr5_3:18005767-18005786 None:intergenic 35.0%
TCTTCTAAATATGTTGCTGC+AGG + chr5_3:18005832-18005851 None:intergenic 35.0%
ACAAAGTGTAAGTCTCAATC+AGG - chr5_3:18005853-18005872 Msa0803040:five_prime_UTR 35.0%
TTCCTTGGTCAATGTAAAGA+AGG - chr5_3:18005877-18005896 Msa0803040:five_prime_UTR 35.0%
TCCTTGGTCAATGTAAAGAA+GGG - chr5_3:18005878-18005897 Msa0803040:five_prime_UTR 35.0%
! ACTCGTCTATCATCTAAAAC+AGG - chr5_3:18005905-18005924 Msa0803040:five_prime_UTR 35.0%
! GTACGGTTATCTTGTTTACT+CGG + chr5_3:18005940-18005959 None:intergenic 35.0%
AGAGACATCAACAAATGAAG+AGG - chr5_3:18006011-18006030 Msa0803040:five_prime_UTR 35.0%
CAACAAATGAAGAGGAAATG+GGG - chr5_3:18006019-18006038 Msa0803040:five_prime_UTR 35.0%
AATGTGTGGACTTTCTCTGA+TGG - chr5_3:18002865-18002884 Msa0803040:intron 40.0%
!! GATTTGTTGTGCGTAATTGG+AGG - chr5_3:18002933-18002952 Msa0803040:intron 40.0%
CCTAATTACTGGAACTGACA+AGG + chr5_3:18003168-18003187 Msa0803030:three_prime_UTR 40.0%
CCTTGTCAGTTCCAGTAATT+AGG - chr5_3:18003165-18003184 Msa0803040:intron 40.0%
! TTTTGACACACCATGTCTGT+CGG - chr5_3:18003445-18003464 Msa0803040:intron 40.0%
! GAAAGCAAAGTAGTGGAAGA+TGG - chr5_3:18003721-18003740 Msa0803040:intron 40.0%
! AAAGTAGTGGAAGATGGGTT+TGG - chr5_3:18003727-18003746 Msa0803040:intron 40.0%
! ACTCATATCGGAAGCATATG+AGG - chr5_3:18003883-18003902 Msa0803040:intron 40.0%
ATACAGTCGGATGAAAACTG+AGG - chr5_3:18004123-18004142 Msa0803040:intron 40.0%
!! AGCTAGGCTTTAAATAGGCT+AGG + chr5_3:18004280-18004299 None:intergenic 40.0%
! AGCCTATTTAAAGCCTAGCT+CGG - chr5_3:18004280-18004299 Msa0803040:intron 40.0%
!! GCGTTGATAATAAACGAGCT+GGG + chr5_3:18004306-18004325 None:intergenic 40.0%
CCTTATAAAAAGCTTGAGCC+TGG + chr5_3:18004328-18004347 None:intergenic 40.0%
! CCAGGCTCAAGCTTTTTATA+AGG - chr5_3:18004325-18004344 Msa0803040:intron 40.0%
!!! CTTTTTTGCAAGCCTTAACC+TGG + chr5_3:18004369-18004388 None:intergenic 40.0%
AAAAGCCTATTAAGCCCATC+TGG - chr5_3:18004384-18004403 Msa0803040:intron 40.0%
!! GTTTTGGCCTATTTAGGAGT+AGG - chr5_3:18004591-18004610 Msa0803040:intron 40.0%
! GCTTCATCAGGCCTTTAAAA+AGG - chr5_3:18004668-18004687 Msa0803040:intron 40.0%
GATTGGATAATGAATAGGGG+TGG + chr5_3:18004809-18004828 None:intergenic 40.0%
AAATAGCGCCACATTCAGAT+TGG + chr5_3:18004826-18004845 None:intergenic 40.0%
GAGTTCCATGTAAGCATCAT+TGG - chr5_3:18004903-18004922 Msa0803040:intron 40.0%
ATGTAAGCATCATTGGTCCA+GGG - chr5_3:18004910-18004929 Msa0803040:intron 40.0%
TCTATCTATGTTGTGGGGAA+TGG - chr5_3:18005210-18005229 Msa0803040:intron 40.0%
CTATCTATGTTGTGGGGAAT+GGG - chr5_3:18005211-18005230 Msa0803040:intron 40.0%
TATCTATGTTGTGGGGAATG+GGG - chr5_3:18005212-18005231 Msa0803040:intron 40.0%
!!! CTTCAGTTTTGAGGGATCAA+TGG + chr5_3:18005511-18005530 None:intergenic 40.0%
! CCTTTCAATGCATTTTCCCT+AGG + chr5_3:18005580-18005599 None:intergenic 40.0%
CCTAGGGAAAATGCATTGAA+AGG - chr5_3:18005577-18005596 Msa0803040:intron 40.0%
!! ATGATGAGTTGATGATCGCA+AGG + chr5_3:18005605-18005624 None:intergenic 40.0%
GAGTTATTAGGCTGCATTGA+TGG + chr5_3:18005669-18005688 None:intergenic 40.0%
CAGAACACAACCATAATCAC+TGG - chr5_3:18005696-18005715 Msa0803040:intron 40.0%
CTGGATAATTAAACAGAGGC+AGG - chr5_3:18005715-18005734 Msa0803040:intron 40.0%
TTGAAATTTCTGTGGGCCTA+TGG - chr5_3:18005797-18005816 Msa0803040:five_prime_UTR 40.0%
TCTAAATATGTTGCTGCAGG+TGG + chr5_3:18005829-18005848 None:intergenic 40.0%
AAGTCTCAATCAGGATTCCT+TGG - chr5_3:18005862-18005881 Msa0803040:five_prime_UTR 40.0%
TCCCTTCTTTACATTGACCA+AGG + chr5_3:18005882-18005901 None:intergenic 40.0%
! AGAGTGTCTACAAACAGAGC+AGG + chr5_3:18002975-18002994 Msa0803030:intron 45.0%
!!! GATTTTCCATGGTTCTAGCC+TGG - chr5_3:18003051-18003070 Msa0803040:intron 45.0%
GTATGTTTGCCTTATCCACC+AGG + chr5_3:18003072-18003091 Msa0803030:CDS 45.0%
GGTGGATAAGGCAAACATAC+AGG - chr5_3:18003072-18003091 Msa0803040:intron 45.0%
!! CTTATCAGGGTGCCATTTCA+TGG + chr5_3:18003825-18003844 None:intergenic 45.0%
AAACTGAGGATCCCCTAAGA+AGG - chr5_3:18004137-18004156 Msa0803040:intron 45.0%
AACTGAGGATCCCCTAAGAA+GGG - chr5_3:18004138-18004157 Msa0803040:intron 45.0%
GTTCCCACCCCTAATAATGA+AGG + chr5_3:18004228-18004247 None:intergenic 45.0%
TCATTATTAGGGGTGGGAAC+AGG - chr5_3:18004228-18004247 Msa0803040:intron 45.0%
CTTTAAATAGGCTAGGCCGT+AGG + chr5_3:18004273-18004292 None:intergenic 45.0%
!! GGCGTTGATAATAAACGAGC+TGG + chr5_3:18004307-18004326 None:intergenic 45.0%
GCTCGTTTATTATCAACGCC+AGG - chr5_3:18004307-18004326 Msa0803040:intron 45.0%
! ATCAGGCCTTTAAAAAGGCC+AGG - chr5_3:18004673-18004692 Msa0803040:intron 45.0%
!! ATAGGCTCATTTTCGAGGTC+TGG + chr5_3:18004699-18004718 None:intergenic 45.0%
CCTCGAAAATGAGCCTATGA+CGG - chr5_3:18004701-18004720 Msa0803040:intron 45.0%
CTCGAAAATGAGCCTATGAC+GGG - chr5_3:18004702-18004721 Msa0803040:intron 45.0%
!! ATGAGCCTATGACGGGTAAT+AGG - chr5_3:18004709-18004728 Msa0803040:intron 45.0%
!!! TTTTGAAGTAGGCCAGGCTT+AGG - chr5_3:18004751-18004770 Msa0803040:intron 45.0%
CTGGACCAATGATGCTTACA+TGG + chr5_3:18004911-18004930 None:intergenic 45.0%
CATGTAAGCATCATTGGTCC+AGG - chr5_3:18004909-18004928 Msa0803040:intron 45.0%
! GTTTTTCCATATACCACGGC+CGG + chr5_3:18004944-18004963 None:intergenic 45.0%
GTTGCACATAGATGATGCCT+TGG - chr5_3:18005026-18005045 Msa0803040:CDS 45.0%
! TTTGGCTTCATCGAGGTTCT+AGG - chr5_3:18005281-18005300 Msa0803040:intron 45.0%
AACTTCTCTACACAGGCCTA+GGG - chr5_3:18005561-18005580 Msa0803040:intron 45.0%
AAATTTCTGTGGGCCTATGG+AGG - chr5_3:18005800-18005819 Msa0803040:five_prime_UTR 45.0%
AAATGAAGAGGAAATGGGGC+AGG - chr5_3:18006023-18006042 Msa0803040:five_prime_UTR 45.0%
AATGAAGAGGAAATGGGGCA+GGG - chr5_3:18006024-18006043 Msa0803040:five_prime_UTR 45.0%
!! TTTCCATGGTTCTAGCCTGG+TGG - chr5_3:18003054-18003073 Msa0803040:intron 50.0%
TATCCACCAGGCTAGAACCA+TGG + chr5_3:18003060-18003079 Msa0803030:CDS 50.0%
!! TGGTTCTAGCCTGGTGGATA+AGG - chr5_3:18003060-18003079 Msa0803040:intron 50.0%
! ACCATGTCTGTCGGTGTTTG+TGG - chr5_3:18003454-18003473 Msa0803040:intron 50.0%
TCCACAAACACCGACAGACA+TGG + chr5_3:18003458-18003477 Msa0803030:intron 50.0%
!! GGCCGAGCTAGGCTTTAAAT+AGG + chr5_3:18004285-18004304 None:intergenic 50.0%
GCTTAATAGGTCGGCCAGAT+GGG + chr5_3:18004401-18004420 None:intergenic 50.0%
AGCTTAATAGGTCGGCCAGA+TGG + chr5_3:18004402-18004421 None:intergenic 50.0%
ATGGGCTTTCAGGCTTCATC+AGG - chr5_3:18004656-18004675 Msa0803040:intron 50.0%
!! GTCTGGCCTGGCCTTTTTAA+AGG + chr5_3:18004682-18004701 None:intergenic 50.0%
! CCGTCATAGGCTCATTTTCG+AGG + chr5_3:18004704-18004723 None:intergenic 50.0%
CTTGGCCTATTACCCGTCAT+AGG + chr5_3:18004717-18004736 None:intergenic 50.0%
! AATAGGGGTGGAAATGGGCT+AGG + chr5_3:18004797-18004816 None:intergenic 50.0%
TTGGTCCAGGGTTTGATCTC+CGG - chr5_3:18004922-18004941 Msa0803040:intron 50.0%
GGACACCAACTTCTCTACAC+AGG - chr5_3:18005554-18005573 Msa0803040:intron 50.0%
CTAGGCCTGTGTAGAGAAGT+TGG + chr5_3:18005562-18005581 None:intergenic 50.0%
CAACTTCTCTACACAGGCCT+AGG - chr5_3:18005560-18005579 Msa0803040:intron 50.0%
! GTCGGCCAGATGGGCTTAAT+AGG + chr5_3:18004392-18004411 None:intergenic 55.0%
!! CTCATTTTCGAGGTCTGGCC+TGG + chr5_3:18004694-18004713 None:intergenic 55.0%
ACGGGTAATAGGCCAAGCTC+AGG - chr5_3:18004720-18004739 Msa0803040:intron 55.0%
! GGCTTTGTAAGGCCTAAGCC+TGG + chr5_3:18004766-18004785 None:intergenic 55.0%
GGCTTAGGCCTTACAAAGCC+CGG - chr5_3:18004766-18004785 Msa0803040:intron 55.0%
TGATCTCCGGCCGTGGTATA+TGG - chr5_3:18004935-18004954 Msa0803040:intron 55.0%
! AACAGAGGCAGGGTGCTTCA+AGG - chr5_3:18005726-18005745 Msa0803040:intron 55.0%
ATTAGGGGTGGGAACAGGCC+AGG - chr5_3:18004233-18004252 Msa0803040:intron 60.0%
TAAACGAGCTGGGCCGAGCT+AGG + chr5_3:18004296-18004315 None:intergenic 60.0%
CTGCAGGTGGTCACCTCCAT+AGG + chr5_3:18005816-18005835 None:intergenic 60.0%
AACAGGCCAGGCCGTTCGAC+AGG - chr5_3:18004245-18004264 Msa0803040:intron 65.0%
ACAGGCCAGGCCGTTCGACA+GGG - chr5_3:18004246-18004265 Msa0803040:intron 65.0%
AGGCCTTACAAAGCCCGGCG+TGG - chr5_3:18004771-18004790 Msa0803040:intron 65.0%
! AGGCCACGCCGGGCTTTGTA+AGG + chr5_3:18004777-18004796 None:intergenic 65.0%
!! GAAATGGGCTAGGCCACGCC+GGG + chr5_3:18004787-18004806 None:intergenic 65.0%
!! GGAAATGGGCTAGGCCACGC+CGG + chr5_3:18004788-18004807 None:intergenic 65.0%
CACGGCCGGAGATCAAACCC+TGG + chr5_3:18004930-18004949 None:intergenic 65.0%
CAGGGTTTGATCTCCGGCCG+TGG - chr5_3:18004928-18004947 Msa0803040:intron 65.0%
CAGGCCAGGCCGTTCGACAG+GGG - chr5_3:18004247-18004266 Msa0803040:intron 70.0%
TAGGCCCCTGTCGAACGGCC+TGG + chr5_3:18004254-18004273 None:intergenic 70.0%
GGCCGTTCGACAGGGGCCTA+CGG - chr5_3:18004254-18004273 Msa0803040:intron 70.0%
GGCCGTAGGCCCCTGTCGAA+CGG + chr5_3:18004259-18004278 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 18002816 18006054 18002816 ID=Msa0803040;Name=Msa0803040
chr5_3 mRNA 18002816 18006054 18002816 ID=Msa0803040-mRNA-1;Parent=Msa0803040;Name=Msa0803040-mRNA-1;_AED=0.34;_eAED=0.34;_QI=354|0.5|0.33|0.66|0|0|3|0|68
chr5_3 exon 18002816 18002857 18002816 ID=Msa0803040-mRNA-1:exon:7429;Parent=Msa0803040-mRNA-1
chr5_3 exon 18004966 18005206 18004966 ID=Msa0803040-mRNA-1:exon:7428;Parent=Msa0803040-mRNA-1
chr5_3 exon 18005777 18006054 18005777 ID=Msa0803040-mRNA-1:exon:7427;Parent=Msa0803040-mRNA-1
chr5_3 five_prime_UTR 18005777 18006054 18005777 ID=Msa0803040-mRNA-1:five_prime_utr;Parent=Msa0803040-mRNA-1
chr5_3 five_prime_UTR 18005131 18005206 18005131 ID=Msa0803040-mRNA-1:five_prime_utr;Parent=Msa0803040-mRNA-1
chr5_3 CDS 18004966 18005130 18004966 ID=Msa0803040-mRNA-1:cds;Parent=Msa0803040-mRNA-1
chr5_3 CDS 18002816 18002857 18002816 ID=Msa0803040-mRNA-1:cds;Parent=Msa0803040-mRNA-1
Gene Sequence

>Msa0803040

ATGGGTTTGGATTACTATGAGATTTTGGAGGTTGACAAGAATGCTACAGATGATGAGTTGCAGAAAGCATATAGAAAACTTGCCATGAAATGGCACCCTGATAAGAATCCTGACAATAAGAATGATGCTGAGACTAAATTCAAACTCATATCGGAAGCATATGAGAGACATCAACAAATGAAGAGGAAATGGGGCAGGGCAAGTTGA
Protein sequence

>Msa0803040

MGLDYYEILEVDKNATDDELQKAYRKLAMKWHPDKNPDNKNDAETKFKLISEAYERHQQMKRKWGRAS*