AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0806840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806840 MtrunA17_Chr5g0421411 98.252 286 5 0 53 338 1 286 0.0 587
Msa0806840 MtrunA17_Chr7g0244461 46.732 306 151 4 32 337 19 312 6.86e-98 292
Msa0806840 MtrunA17_Chr5g0404721 48.000 300 152 3 38 337 35 330 1.36e-97 292
Msa0806840 MtrunA17_Chr3g0106921 46.084 332 155 7 11 337 8 320 1.26e-96 289
Msa0806840 MtrunA17_Chr5g0432441 47.904 334 149 9 10 337 12 326 9.85e-96 287
Msa0806840 MtrunA17_Chr2g0320051 44.073 329 166 5 9 337 8 318 5.17e-94 282
Msa0806840 MtrunA17_Chr5g0400331 47.267 311 153 4 33 337 26 331 5.65e-94 283
Msa0806840 MtrunA17_Chr8g0372601 46.535 303 154 5 38 338 35 331 1.20e-93 282
Msa0806840 MtrunA17_Chr2g0292091 45.397 315 163 4 26 337 17 325 2.62e-93 281
Msa0806840 MtrunA17_Chr2g0320071 42.643 333 172 5 5 337 4 317 6.57e-93 279
Msa0806840 MtrunA17_Chr3g0104101 92.199 141 11 0 198 338 10 150 1.26e-92 272
Msa0806840 MtrunA17_Chr3g0114451 45.955 309 155 4 29 337 22 318 1.35e-92 278
Msa0806840 MtrunA17_Chr7g0244451 43.865 326 166 5 12 337 8 316 1.91e-92 278
Msa0806840 MtrunA17_Chr5g0438021 43.558 326 172 4 13 337 11 325 2.69e-92 278
Msa0806840 MtrunA17_Chr7g0224371 43.844 333 161 8 9 337 12 322 3.42e-92 278
Msa0806840 MtrunA17_Chr2g0320101 43.871 310 162 4 28 337 20 317 7.58e-92 276
Msa0806840 MtrunA17_Chr2g0320061 44.516 310 161 5 28 337 20 318 2.42e-91 275
Msa0806840 MtrunA17_Chr3g0129051 43.373 332 171 6 7 337 7 322 3.94e-91 275
Msa0806840 MtrunA17_Chr4g0013171 48.387 310 140 8 33 337 27 321 6.08e-91 275
Msa0806840 MtrunA17_Chr4g0068621 47.573 309 148 7 33 337 38 336 6.41e-91 275
Msa0806840 MtrunA17_Chr1g0155611 45.902 305 162 3 33 337 46 347 6.83e-91 275
Msa0806840 MtrunA17_Chr2g0292251 44.728 313 168 5 27 338 22 330 2.65e-90 274
Msa0806840 MtrunA17_Chr2g0292181 45.367 313 166 5 27 338 32 340 3.17e-89 271
Msa0806840 MtrunA17_Chr4g0070951 43.505 331 174 5 9 338 12 330 6.86e-89 270
Msa0806840 MtrunA17_Chr2g0292151 45.231 325 169 6 14 337 14 330 2.63e-88 269
Msa0806840 MtrunA17_Chr4g0013191 46.128 297 148 4 42 337 55 340 5.80e-88 268
Msa0806840 MtrunA17_Chr2g0292211 44.681 329 167 7 14 338 25 342 6.57e-88 268
Msa0806840 MtrunA17_Chr2g0320091 44.051 311 161 5 28 337 20 318 1.60e-87 266
Msa0806840 MtrunA17_Chr5g0402571 45.603 307 160 5 32 337 26 326 2.67e-87 265
Msa0806840 MtrunA17_Chr2g0299691 45.062 324 172 4 17 338 40 359 2.92e-87 266
Msa0806840 MtrunA17_Chr4g0038161 44.345 336 164 7 5 337 5 320 3.60e-87 265
Msa0806840 MtrunA17_Chr2g0292111 44.000 325 173 6 14 337 14 330 3.66e-87 266
Msa0806840 MtrunA17_Chr5g0402581 45.928 307 159 5 32 337 26 326 6.09e-87 265
Msa0806840 MtrunA17_Chr5g0432351 44.611 334 167 9 7 337 3 321 5.29e-86 262
Msa0806840 MtrunA17_Chr4g0013201 45.098 306 156 5 32 337 45 338 7.49e-86 262
Msa0806840 MtrunA17_Chr2g0292241 43.865 326 174 6 14 338 14 331 7.76e-86 263
Msa0806840 MtrunA17_Chr4g0038151 44.268 314 155 6 30 337 22 321 1.21e-85 261
Msa0806840 MtrunA17_Chr5g0432341 45.210 334 165 9 7 337 3 321 2.47e-85 260
Msa0806840 MtrunA17_Chr4g0043391 43.226 310 165 4 30 337 24 324 2.52e-85 260
Msa0806840 MtrunA17_Chr3g0106901 44.479 326 167 5 13 337 11 323 3.24e-85 260
Msa0806840 MtrunA17_Chr4g0013181 46.053 304 150 6 36 337 62 353 7.33e-85 260
Msa0806840 MtrunA17_Chr7g0258811 41.593 339 183 8 3 337 44 371 1.19e-84 260
Msa0806840 MtrunA17_Chr2g0320031 43.137 306 163 3 32 337 25 319 1.39e-84 258
Msa0806840 MtrunA17_Chr2g0292291 42.331 326 179 5 14 338 16 333 1.56e-84 259
Msa0806840 MtrunA17_Chr5g0432331 46.278 309 152 8 32 337 55 352 3.79e-84 258
Msa0806840 MtrunA17_Chr3g0129041 42.470 332 174 6 7 337 7 322 5.03e-84 257
Msa0806840 MtrunA17_Chr4g0038171 42.903 310 166 4 30 337 21 321 5.21e-84 257
Msa0806840 MtrunA17_Chr3g0129061 41.867 332 176 6 7 337 7 322 1.10e-83 256
Msa0806840 MtrunA17_Chr2g0291751 42.769 325 177 6 14 337 14 330 1.37e-83 257
Msa0806840 MtrunA17_Chr4g0013721 44.695 311 162 5 28 337 95 396 1.61e-83 259
Msa0806840 MtrunA17_Chr4g0013821 44.040 302 159 5 37 337 32 324 2.91e-83 256
Msa0806840 MtrunA17_Chr2g0292101 43.030 330 177 6 12 337 4 326 5.08e-83 255
Msa0806840 MtrunA17_Chr2g0292261 41.056 341 186 7 1 337 1 330 1.28e-82 254
Msa0806840 MtrunA17_Chr2g0292271 42.388 335 180 6 6 337 6 330 2.23e-82 254
Msa0806840 MtrunA17_Chr1g0175561 42.357 314 175 6 26 337 17 326 2.44e-82 253
Msa0806840 MtrunA17_Chr1g0213061 41.033 329 176 8 11 337 9 321 5.60e-82 252
Msa0806840 MtrunA17_Chr3g0107881 45.687 313 155 8 29 337 23 324 5.62e-81 249
Msa0806840 MtrunA17_Chr6g0477071 42.623 305 159 7 33 337 54 342 5.63e-79 244
Msa0806840 MtrunA17_Chr8g0371381 42.997 307 160 8 33 337 26 319 4.58e-77 239
Msa0806840 MtrunA17_Chr1g0188431 42.295 305 160 7 33 337 39 327 6.94e-77 239
Msa0806840 MtrunA17_Chr5g0405991 39.818 329 179 8 10 337 6 316 5.37e-76 236
Msa0806840 MtrunA17_Chr1g0204011 39.583 336 187 8 3 337 2 322 2.86e-75 234
Msa0806840 MtrunA17_Chr4g0024121 39.264 326 184 8 14 337 13 326 3.02e-74 232
Msa0806840 MtrunA17_Chr1g0157051 40.789 304 170 5 33 336 24 317 5.39e-72 226
Msa0806840 MtrunA17_Chr1g0194151 41.325 317 171 7 28 337 25 333 6.04e-69 218
Msa0806840 MtrunA17_Chr3g0128061 38.554 332 194 5 8 338 5 327 9.47e-69 218
Msa0806840 MtrunA17_Chr2g0322861 38.019 313 183 9 28 337 82 386 3.06e-68 218
Msa0806840 MtrunA17_Chr2g0279041 41.311 305 163 9 38 337 32 325 4.00e-68 216
Msa0806840 MtrunA17_Chr4g0063671 40.190 316 172 9 28 337 16 320 6.81e-68 215
Msa0806840 MtrunA17_Chr4g0076211 38.689 305 173 6 34 337 42 333 2.10e-67 214
Msa0806840 MtrunA17_Chr4g0046131 37.908 306 182 5 32 333 41 342 9.72e-65 208
Msa0806840 MtrunA17_Chr1g0197411 37.662 308 184 6 29 333 24 326 1.31e-64 207
Msa0806840 MtrunA17_Chr1g0194271 39.077 325 174 8 28 337 25 340 3.22e-64 207
Msa0806840 MtrunA17_Chr5g0432401 37.462 331 148 8 9 336 11 285 5.59e-64 204
Msa0806840 MtrunA17_Chr1g0164891 40.461 304 172 7 38 337 94 392 5.75e-64 207
Msa0806840 MtrunA17_Chr2g0296621 39.216 306 171 7 33 337 50 341 2.96e-63 204
Msa0806840 MtrunA17_Chr4g0075241 32.432 333 211 4 5 337 9 327 4.05e-62 201
Msa0806840 MtrunA17_Chr2g0329641 36.593 317 178 10 30 337 33 335 1.45e-60 197
Msa0806840 MtrunA17_Chr7g0254231 35.065 308 181 8 33 338 22 312 1.75e-60 196
Msa0806840 MtrunA17_Chr6g0451571 36.336 333 192 8 9 337 18 334 2.63e-60 196
Msa0806840 MtrunA17_Chr7g0268501 36.119 335 191 11 8 337 4 320 1.81e-59 194
Msa0806840 MtrunA17_Chr7g0268521 36.119 335 191 11 8 337 4 320 1.81e-59 194
Msa0806840 MtrunA17_Chr6g0467161 35.328 351 190 11 1 338 11 337 1.94e-59 194
Msa0806840 MtrunA17_Chr8g0364291 35.577 312 192 7 29 337 19 324 4.56e-59 193
Msa0806840 MtrunA17_Chr2g0316791 36.336 333 174 9 33 333 76 402 2.94e-58 193
Msa0806840 MtrunA17_Chr6g0467121 35.821 335 192 9 9 337 46 363 9.02e-58 191
Msa0806840 MtrunA17_Chr1g0148021 36.624 314 180 9 32 337 26 328 1.23e-57 189
Msa0806840 MtrunA17_Chr2g0316481 36.036 333 175 9 33 333 76 402 2.65e-57 191
Msa0806840 MtrunA17_Chr5g0401431 33.634 333 206 9 9 336 6 328 5.10e-57 187
Msa0806840 MtrunA17_Chr7g0225221 35.435 333 195 9 8 337 5 320 3.89e-56 185
Msa0806840 MtrunA17_Chr2g0278671 38.037 326 165 11 28 337 19 323 4.90e-56 185
Msa0806840 MtrunA17_Chr2g0316831 36.036 333 175 9 33 333 112 438 6.29e-56 188
Msa0806840 MtrunA17_Chr6g0467251 36.254 331 194 7 7 333 7 324 6.83e-56 185
Msa0806840 MtrunA17_Chr2g0316191 35.928 334 175 10 33 333 76 403 7.87e-56 187
Msa0806840 MtrunA17_Chr6g0460711 35.780 327 189 8 14 337 6 314 8.35e-56 184
Msa0806840 MtrunA17_Chr7g0254201 34.295 312 179 9 33 337 20 312 9.18e-56 184
Msa0806840 MtrunA17_Chr2g0279061 41.353 266 140 9 77 337 12 266 1.08e-54 179
Msa0806840 MtrunA17_Chr2g0311251 34.226 336 182 10 33 336 11 339 2.53e-51 173
Msa0806840 MtrunA17_Chr4g0072091 35.294 306 185 8 32 333 23 319 3.32e-51 172
Msa0806840 MtrunA17_Chr1g0181431 32.039 309 198 5 32 338 26 324 1.12e-50 171
Msa0806840 MtrunA17_Chr4g0053871 33.762 311 191 7 27 333 21 320 1.67e-50 171
Msa0806840 MtrunA17_Chr4g0074851 31.429 315 198 6 28 337 27 328 3.20e-49 167
Msa0806840 MtrunA17_Chr1g0181481 31.715 309 199 5 32 338 26 324 3.97e-49 167
Msa0806840 MtrunA17_Chr5g0413051 35.275 309 182 10 38 337 78 377 4.46e-49 168
Msa0806840 MtrunA17_Chr1g0181511 32.581 310 195 7 32 338 26 324 6.21e-49 166
Msa0806840 MtrunA17_Chr3g0136751 33.631 336 199 8 7 337 6 322 1.16e-48 166
Msa0806840 MtrunA17_Chr2g0311241 31.746 378 170 13 33 333 60 426 2.90e-48 167
Msa0806840 MtrunA17_Chr3g0114461 42.211 199 104 5 139 337 5 192 1.57e-43 148
Msa0806840 MtrunA17_Chr2g0316811 37.603 242 129 6 33 256 50 287 4.04e-42 149
Msa0806840 MtrunA17_Chr2g0292281 39.352 216 114 7 125 338 17 217 6.15e-40 140
Msa0806840 MtrunA17_Chr4g0004591 29.595 321 161 7 33 333 77 352 7.18e-36 133
Msa0806840 MtrunA17_Chr3g0114491 51.754 114 55 0 30 143 15 128 1.47e-35 126
Msa0806840 MtrunA17_Chr2g0316141 33.333 276 162 10 79 338 11 280 1.73e-34 127
Msa0806840 MtrunA17_Chr2g0316181 33.333 276 162 10 79 338 11 280 1.73e-34 127
Msa0806840 MtrunA17_Chr5g0432321 46.032 126 57 5 154 278 17 132 5.79e-25 98.2
Msa0806840 MtrunA17_Chr6g0468731 56.944 72 27 2 72 143 22 89 3.90e-20 84.0
Msa0806840 MtrunA17_Chr1g0181491 36.207 116 68 3 30 143 9 120 3.14e-19 83.2
Msa0806840 MtrunA17_Chr7g0254181 38.095 105 61 2 95 199 22 122 3.43e-17 77.8
Msa0806840 MtrunA17_Chr1g0181501 29.126 206 118 7 143 338 3 190 4.85e-16 75.5
Msa0806840 MtrunA17_Chr4g0013811 43.421 76 43 0 140 215 2 77 2.03e-15 70.5
Msa0806840 MtrunA17_Chr1g0196191 51.429 70 33 1 71 140 23 91 1.20e-14 70.5
Msa0806840 MtrunA17_Chr5g0432361 41.304 92 51 3 245 336 20 108 5.08e-14 67.4
Msa0806840 MtrunA17_Chr7g0254171 33.654 104 65 2 78 178 3 105 9.63e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806840 AT1G68850.1 71.661 307 86 1 33 338 30 336 5.39e-171 479
Msa0806840 AT4G36430.1 48.344 302 152 3 37 338 34 331 3.50e-102 304
Msa0806840 AT2G18150.1 48.000 300 152 3 38 337 41 336 3.88e-100 299
Msa0806840 AT2G18140.1 47.682 302 154 3 36 337 38 335 1.26e-96 290
Msa0806840 AT4G16270.1 49.502 301 144 5 38 337 55 348 1.20e-94 285
Msa0806840 AT1G44970.1 47.667 300 154 3 38 337 50 346 1.45e-94 285
Msa0806840 AT5G19890.1 47.284 313 148 8 30 337 27 327 1.82e-94 284
Msa0806840 AT3G50990.1 44.839 310 167 3 28 337 36 341 1.06e-93 283
Msa0806840 AT5G05340.1 45.110 317 161 6 22 337 20 324 7.60e-93 280
Msa0806840 AT2G38380.1 45.338 311 164 6 29 337 27 333 1.47e-87 267
Msa0806840 AT5G06720.1 41.212 330 184 6 9 337 13 333 1.53e-87 266
Msa0806840 AT2G22420.1 45.333 300 156 3 38 337 28 319 4.87e-87 265
Msa0806840 AT2G35380.1 43.402 341 173 6 7 337 5 335 4.93e-87 265
Msa0806840 AT5G58400.1 43.544 333 168 7 7 337 11 325 5.45e-87 265
Msa0806840 AT3G32980.1 45.192 312 166 5 29 338 27 335 5.59e-87 266
Msa0806840 AT5G66390.1 41.720 314 179 3 24 337 24 333 5.93e-87 265
Msa0806840 AT5G06730.1 40.303 330 187 6 9 337 14 334 8.63e-87 265
Msa0806840 AT2G38390.1 43.408 311 170 6 29 337 27 333 4.27e-86 263
Msa0806840 AT5G58390.1 43.373 332 168 7 8 337 3 316 1.32e-85 261
Msa0806840 AT3G49120.1 43.590 312 171 5 29 338 28 336 1.18e-84 260
Msa0806840 AT3G49110.1 42.949 312 173 5 29 338 29 337 2.50e-82 254
Msa0806840 AT4G08770.1 42.308 312 175 5 29 338 20 328 2.68e-82 253
Msa0806840 AT1G14540.1 41.987 312 165 4 29 337 17 315 4.86e-82 252
Msa0806840 AT4G08780.1 41.667 312 177 5 29 338 20 328 1.69e-81 251
Msa0806840 AT1G14550.1 42.949 312 161 7 30 337 23 321 1.48e-80 248
Msa0806840 AT1G49570.1 42.581 310 164 5 32 335 47 348 5.57e-80 248
Msa0806840 AT5G19880.1 43.109 341 171 9 6 337 3 329 6.75e-77 239
Msa0806840 AT2G41480.1 40.118 339 183 9 5 337 4 328 1.31e-74 233
Msa0806840 AT4G11290.1 39.423 312 180 7 29 337 21 326 8.14e-73 229
Msa0806840 AT5G51890.1 41.479 311 170 8 29 337 22 322 4.34e-72 226
Msa0806840 AT4G33420.1 38.739 333 178 9 6 337 18 325 5.86e-71 224
Msa0806840 AT1G05260.1 37.742 310 186 6 30 338 23 326 9.30e-71 223
Msa0806840 AT4G33420.2 38.739 333 178 9 6 337 65 372 3.06e-70 223
Msa0806840 AT5G64120.1 41.528 301 163 8 38 337 40 328 7.85e-69 218
Msa0806840 AT1G71695.1 38.387 310 174 7 33 338 44 340 3.98e-68 218
Msa0806840 AT5G42180.1 39.145 304 175 5 33 336 23 316 9.63e-67 213
Msa0806840 AT3G03670.1 39.355 310 174 7 32 337 22 321 1.80e-65 209
Msa0806840 AT3G21770.1 36.452 310 188 7 26 333 22 324 7.46e-65 208
Msa0806840 AT5G40150.1 37.267 322 172 9 27 337 26 328 7.66e-65 208
Msa0806840 AT2G34060.1 37.419 310 180 5 32 334 41 343 2.27e-64 207
Msa0806840 AT3G28200.1 37.621 311 181 5 29 337 17 316 5.14e-64 206
Msa0806840 AT5G39580.1 40.728 302 165 9 38 337 30 319 8.90e-64 205
Msa0806840 AT2G35380.2 44.177 249 128 3 95 337 4 247 3.19e-63 201
Msa0806840 AT1G05240.1 37.898 314 177 9 31 337 23 325 7.16e-63 203
Msa0806840 AT1G05250.1 37.898 314 177 9 31 337 23 325 7.16e-63 203
Msa0806840 AT4G17690.1 36.909 317 178 6 28 337 21 322 2.09e-62 202
Msa0806840 AT5G17820.1 38.111 307 173 9 32 337 23 313 5.97e-62 200
Msa0806840 AT5G47000.1 37.987 308 178 7 33 337 33 330 2.80e-61 199
Msa0806840 AT5G15180.1 36.686 338 192 11 6 337 8 329 3.43e-61 199
Msa0806840 AT5G17820.2 38.111 307 173 9 32 337 76 366 1.10e-60 199
Msa0806840 AT4G33870.1 33.243 367 192 10 3 333 48 397 7.46e-60 197
Msa0806840 AT5G64110.1 38.264 311 176 8 31 337 32 330 1.02e-59 195
Msa0806840 AT3G01190.1 37.273 330 183 11 12 337 12 321 2.26e-59 194
Msa0806840 AT5G14130.1 34.146 328 202 8 14 337 13 330 3.30e-58 191
Msa0806840 AT5G64100.1 38.816 304 168 9 38 337 42 331 6.31e-58 190
Msa0806840 AT5G14130.2 34.146 328 202 8 14 337 54 371 8.49e-58 191
Msa0806840 AT1G30870.1 38.710 310 172 9 33 338 51 346 3.85e-57 189
Msa0806840 AT4G37530.1 36.102 313 182 6 29 333 23 325 1.63e-56 187
Msa0806840 AT1G24110.1 36.039 308 185 6 30 333 19 318 2.19e-56 186
Msa0806840 AT4G25980.1 35.897 312 182 9 33 337 26 326 5.49e-56 185
Msa0806840 AT1G77100.1 35.629 334 191 12 10 337 4 319 1.18e-55 184
Msa0806840 AT4G37520.1 34.839 310 184 6 32 333 26 325 2.12e-55 184
Msa0806840 AT4G21960.1 33.439 314 191 6 29 337 27 327 4.23e-55 183
Msa0806840 AT2G24800.1 36.218 312 181 6 32 337 30 329 8.07e-55 182
Msa0806840 AT4G31760.1 34.242 330 201 7 10 337 11 326 2.04e-54 181
Msa0806840 AT4G26010.1 34.251 327 188 9 14 337 7 309 4.88e-54 180
Msa0806840 AT2G39040.1 36.593 317 183 7 30 337 43 350 5.19e-54 181
Msa0806840 AT4G26010.2 34.251 327 188 9 14 337 35 337 1.38e-53 179
Msa0806840 AT2G43480.1 34.870 347 196 11 9 337 1 335 2.30e-53 179
Msa0806840 AT4G37520.2 34.194 310 183 7 32 333 26 322 8.72e-53 177
Msa0806840 AT2G18980.1 35.202 321 172 9 32 337 24 323 8.97e-52 174
Msa0806840 AT4G37530.2 38.023 263 149 4 29 283 23 279 2.55e-51 172
Msa0806840 AT3G49960.1 34.049 326 203 7 14 333 6 325 3.49e-51 173
Msa0806840 AT4G30170.1 33.861 316 183 6 32 337 26 325 4.34e-51 172
Msa0806840 AT5G22410.1 35.831 307 182 9 32 334 27 322 8.71e-51 172
Msa0806840 AT2G37130.1 32.581 310 194 7 32 337 29 327 1.87e-49 168
Msa0806840 AT3G17070.1 31.642 335 210 7 11 337 16 339 2.42e-49 168
Msa0806840 AT2G37130.2 32.573 307 192 7 35 337 1 296 6.12e-49 166
Msa0806840 AT5G24070.1 34.194 310 183 10 37 337 38 335 8.16e-49 167
Msa0806840 AT1G34510.1 35.065 308 179 8 32 337 21 309 1.04e-48 166
Msa0806840 AT5G67400.1 32.353 306 197 7 32 333 26 325 1.66e-48 166
Msa0806840 AT5G39580.2 46.341 164 83 3 38 201 30 188 4.25e-41 144
Msa0806840 AT4G25980.2 39.535 172 100 2 29 200 67 234 1.53e-39 141
Msa0806840 AT2G24800.2 41.667 168 94 1 32 199 30 193 5.72e-36 130
Msa0806840 AT3G17070.2 31.365 271 166 8 75 337 18 276 2.55e-34 127
Msa0806840 AT3G42570.1 36.842 95 59 1 105 198 53 147 2.46e-13 67.4

Find 66 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGATTCAATGGTCAAAA+TGG 0.150346 5_3:-27327399 Msa0806840:CDS
TAGGCCTTGATAAAGAAATT+TGG 0.197512 5_3:+27328512 None:intergenic
GATGATACATACACACTTAA+AGG 0.209734 5_3:-27328887 Msa0806840:CDS
TTCTATCAGTTACTATTAAA+AGG 0.244929 5_3:-27327533 Msa0806840:CDS
CTTCCATGACTGCTTTATTC+AGG 0.245006 5_3:-27329382 Msa0806840:intron
TTGGTGGACAAATAGATTTC+AGG 0.248040 5_3:+27327621 None:intergenic
ACCAACATCCACTCTTTAAA+AGG 0.265822 5_3:-27328848 Msa0806840:CDS
GCTTCATCACTGGAGAAATT+AGG 0.312437 5_3:-27327355 Msa0806840:CDS
AAATCTATTTGTCCACCAAT+TGG 0.335126 5_3:-27327617 Msa0806840:CDS
TTCATCAGGTGTTGGAAGAT+TGG 0.344913 5_3:+27328554 None:intergenic
CCATTGAATCAGAGAATTGC+TGG 0.348685 5_3:+27327408 None:intergenic
AGGCCTTGATAAAGAAATTT+GGG 0.352606 5_3:+27328513 None:intergenic
GGCTAGAGATGCTGTAATTC+TGG 0.365262 5_3:-27328741 Msa0806840:intron
AATCTATTTGTCCACCAATT+GGG 0.367894 5_3:-27327616 Msa0806840:CDS
TGTAGGTTGGTGGACCATAT+TGG 0.381231 5_3:-27328632 Msa0806840:intron
TTGAACTGATTGTCTGAGAT+TGG 0.384305 5_3:+27327647 None:intergenic
ATTCCCAAATTTCTTTATCA+AGG 0.393262 5_3:-27328516 Msa0806840:CDS
CTACTGTGTTTGACATTGTT+AGG 0.395693 5_3:-27329467 Msa0806840:CDS
GTAGGTTGGTGGACCATATT+GGG 0.400494 5_3:-27328631 Msa0806840:intron
CCAGCAATTCTCTGATTCAA+TGG 0.417000 5_3:-27327408 Msa0806840:CDS
AGGCCTATCAGTCACGGATA+TGG 0.421834 5_3:-27328496 Msa0806840:CDS
CAACCATATCCGTGACTGAT+AGG 0.423843 5_3:+27328493 None:intergenic
ACGGATATGGTTGCCCTTGT+CGG 0.453404 5_3:-27328483 Msa0806840:intron
AAAGAATTGTCGAAGAGATT+AGG 0.456399 5_3:+27327554 None:intergenic
GTAACGCAGCCTCGGTTGTT+CGG 0.456843 5_3:-27329410 Msa0806840:CDS
CCCACAGGAACATCCCAATA+TGG 0.467663 5_3:+27328618 None:intergenic
TCTGATTCAATGGTCAAAAT+GGG 0.468708 5_3:-27327398 Msa0806840:CDS
TGACATTGTTAGGAAGCAAA+TGG 0.473769 5_3:-27329457 Msa0806840:CDS
ATCTATTTGTCCACCAATTG+GGG 0.476877 5_3:-27327615 Msa0806840:CDS
GAAATGTACTCGAGTGTGTT+CGG 0.477719 5_3:-27327485 Msa0806840:CDS
TAGGAAGCAAATGGAATGTT+CGG 0.486422 5_3:-27329448 Msa0806840:CDS
ATAGAAACAAGGCTTTCATC+AGG 0.515660 5_3:+27328540 None:intergenic
ACAAGGCTTTCATCAGGTGT+TGG 0.523953 5_3:+27328546 None:intergenic
AATTCAGAAAGCTTCATCAC+TGG 0.525241 5_3:-27327365 Msa0806840:CDS
ATCAGGTGTTGGAAGATTGG+TGG 0.526275 5_3:+27328557 None:intergenic
CTCTAATAATACACTAACAA+TGG 0.528798 5_3:-27329613 Msa0806840:exon
TCAGCACAAGAAACAACTCC+AGG 0.529028 5_3:+27328779 None:intergenic
TGAACCTCGTAACGCAGCCT+CGG 0.534467 5_3:-27329418 Msa0806840:CDS
AGACCTGAATAAAGCAGTCA+TGG 0.539942 5_3:+27329379 None:intergenic
TTTGTTGAATCAGAGTGCCC+TGG 0.544131 5_3:-27328797 Msa0806840:CDS
TGTTCAGGGTTGCGATGGAT+CGG 0.545298 5_3:-27328918 Msa0806840:intron
TGCTGATATTCTCACAATTG+CGG 0.553704 5_3:-27328762 Msa0806840:CDS
TTGACACCTTGCCATACCAA+TGG 0.555332 5_3:+27327721 None:intergenic
TTATGTGTTCAGGGTTGCGA+TGG 0.568814 5_3:-27328923 Msa0806840:intron
CATGCAGGATCCCACACCAT+TGG 0.575994 5_3:-27327737 Msa0806840:intron
AGGATCCCACACCATTGGTA+TGG 0.577138 5_3:-27327732 Msa0806840:CDS
ATTGGGATGTTCCTGTGGGA+AGG 0.579787 5_3:-27328614 Msa0806840:CDS
AGACAATAACATAACAGCAA+TGG 0.581171 5_3:-27327588 Msa0806840:CDS
ACCATATTGGGATGTTCCTG+TGG 0.584136 5_3:-27328619 Msa0806840:CDS
TTATCAAGGCCTATCAGTCA+CGG 0.585184 5_3:-27328502 Msa0806840:CDS
AATAAGAAACATACCGACAA+GGG 0.588494 5_3:+27328470 None:intergenic
TCAGTTACTATTAAAAGGCG+AGG 0.588718 5_3:-27327528 Msa0806840:CDS
ACAGAATCTTTCCTTCCCAC+AGG 0.589625 5_3:+27328603 None:intergenic
CCCACACCATTGGTATGGCA+AGG 0.602364 5_3:-27327727 Msa0806840:CDS
CCTTGCCATACCAATGGTGT+GGG 0.603799 5_3:+27327727 None:intergenic
CTAACAATGTCAAACACAGT+AGG 0.608651 5_3:+27329468 None:intergenic
CAGTTACTATTAAAAGGCGA+GGG 0.613439 5_3:-27327527 Msa0806840:CDS
ACCTTGCCATACCAATGGTG+TGG 0.635221 5_3:+27327726 None:intergenic
ATTTGGGAATGATAGAAACA+AGG 0.637153 5_3:+27328529 None:intergenic
ACAACCGAGGCTGCGTTACG+AGG 0.637956 5_3:+27329414 None:intergenic
TAGTGATCAAGTGTTAGTTG+AGG 0.665046 5_3:+27329504 None:intergenic
GAAGTGAAGCCGAACAACCG+AGG 0.665422 5_3:+27329401 None:intergenic
TCTATTTGTCCACCAATTGG+GGG 0.671500 5_3:-27327614 Msa0806840:CDS
CCATATTGGGATGTTCCTGT+GGG 0.672124 5_3:-27328618 Msa0806840:CDS
TAATAAGAAACATACCGACA+AGG 0.676698 5_3:+27328469 None:intergenic
CAGCACAAGAAACAACTCCA+GGG 0.694829 5_3:+27328780 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTAATTTACAACTT+AGG - chr5_3:27328509-27328528 Msa0806840:CDS 10.0%
!!! TATTAGTTTGAAAAAAAAAA+TGG - chr5_3:27328698-27328717 Msa0806840:intron 10.0%
!! TTATTATTAATTTACAACTT+AGG - chr5_3:27328509-27328528 Msa0806840:CDS 10.0%
!!! TATTAGTTTGAAAAAAAAAA+TGG - chr5_3:27328698-27328717 Msa0806840:intron 10.0%
!!! AAAACAAATTTTGACAGAAA+TGG + chr5_3:27327379-27327398 None:intergenic 20.0%
!!! TTACATATTGTTCTTTTGTT+TGG - chr5_3:27327400-27327419 Msa0806840:CDS 20.0%
!!! AATATGTCTAACTTTGAAAT+AGG + chr5_3:27327676-27327695 None:intergenic 20.0%
!! CAAATTTATCTCTCAATTAA+AGG + chr5_3:27327791-27327810 None:intergenic 20.0%
!! CTTTAATTGAGAGATAAATT+TGG - chr5_3:27327789-27327808 Msa0806840:intron 20.0%
!! AAAAATTTCAGAAGCTAATA+TGG - chr5_3:27327821-27327840 Msa0806840:intron 20.0%
!!! TAATGTAGCTTTACTATATT+TGG + chr5_3:27327947-27327966 None:intergenic 20.0%
!! CTTTGTCTTATATAAGTTAT+TGG - chr5_3:27328275-27328294 Msa0806840:intron 20.0%
!! ATCATAGGAAAATTATATTG+TGG - chr5_3:27328545-27328564 Msa0806840:CDS 20.0%
!! ATTATTTATGCAATGCAATT+TGG + chr5_3:27329060-27329079 None:intergenic 20.0%
!!! AATTTTCGATCAAGAATATA+TGG - chr5_3:27329263-27329282 Msa0806840:intron 20.0%
!! TTCTATCAGTTACTATTAAA+AGG - chr5_3:27329428-27329447 Msa0806840:CDS 20.0%
!!! AAAACAAATTTTGACAGAAA+TGG + chr5_3:27327379-27327398 None:intergenic 20.0%
!!! TTACATATTGTTCTTTTGTT+TGG - chr5_3:27327400-27327419 Msa0806840:CDS 20.0%
!!! AATATGTCTAACTTTGAAAT+AGG + chr5_3:27327676-27327695 None:intergenic 20.0%
!! CAAATTTATCTCTCAATTAA+AGG + chr5_3:27327791-27327810 None:intergenic 20.0%
!! CTTTAATTGAGAGATAAATT+TGG - chr5_3:27327789-27327808 Msa0806840:intron 20.0%
!! AAAAATTTCAGAAGCTAATA+TGG - chr5_3:27327821-27327840 Msa0806840:intron 20.0%
!!! TAATGTAGCTTTACTATATT+TGG + chr5_3:27327947-27327966 None:intergenic 20.0%
!! CTTTGTCTTATATAAGTTAT+TGG - chr5_3:27328275-27328294 Msa0806840:intron 20.0%
!! ATCATAGGAAAATTATATTG+TGG - chr5_3:27328545-27328564 Msa0806840:CDS 20.0%
!! ATTATTTATGCAATGCAATT+TGG + chr5_3:27329060-27329079 None:intergenic 20.0%
!!! AATTTTCGATCAAGAATATA+TGG - chr5_3:27329263-27329282 Msa0806840:intron 20.0%
!! TTCTATCAGTTACTATTAAA+AGG - chr5_3:27329428-27329447 Msa0806840:CDS 20.0%
! CTCTAATAATACACTAACAA+TGG - chr5_3:27327348-27327367 Msa0806840:CDS 25.0%
! GCAATAATTACAAGGAAAAA+AGG + chr5_3:27327618-27327637 None:intergenic 25.0%
! AAACAGAAGCAATAATTACA+AGG + chr5_3:27327626-27327645 None:intergenic 25.0%
!!! GGTTGTGATTTAATTTTTGA+AGG - chr5_3:27327842-27327861 Msa0806840:intron 25.0%
! ATCAAAATGTTGAATGAAAC+AGG - chr5_3:27327877-27327896 Msa0806840:intron 25.0%
!! CTATATTTGGAGTAAAACTT+AGG + chr5_3:27327934-27327953 None:intergenic 25.0%
!! ATTTTCTTTATCTCCTCAAT+AGG + chr5_3:27327997-27328016 None:intergenic 25.0%
!!! TTTGTCCTTTGATTTTTTGT+AGG - chr5_3:27328312-27328331 Msa0806840:intron 25.0%
! ATTCCCAAATTTCTTTATCA+AGG - chr5_3:27328445-27328464 Msa0806840:intron 25.0%
! TGTTTGTTCTACAAAAACAA+AGG + chr5_3:27328765-27328784 None:intergenic 25.0%
! GAATGATCATAAATCAAAGA+AGG + chr5_3:27328915-27328934 None:intergenic 25.0%
!!! AATTACTTTTACAACTACCT+AGG + chr5_3:27329158-27329177 None:intergenic 25.0%
! CTCTAATAATACACTAACAA+TGG - chr5_3:27327348-27327367 Msa0806840:CDS 25.0%
! GCAATAATTACAAGGAAAAA+AGG + chr5_3:27327618-27327637 None:intergenic 25.0%
! AAACAGAAGCAATAATTACA+AGG + chr5_3:27327626-27327645 None:intergenic 25.0%
!!! GGTTGTGATTTAATTTTTGA+AGG - chr5_3:27327842-27327861 Msa0806840:intron 25.0%
! ATCAAAATGTTGAATGAAAC+AGG - chr5_3:27327877-27327896 Msa0806840:intron 25.0%
!! CTATATTTGGAGTAAAACTT+AGG + chr5_3:27327934-27327953 None:intergenic 25.0%
!! ATTTTCTTTATCTCCTCAAT+AGG + chr5_3:27327997-27328016 None:intergenic 25.0%
!!! TTTGTCCTTTGATTTTTTGT+AGG - chr5_3:27328312-27328331 Msa0806840:intron 25.0%
! ATTCCCAAATTTCTTTATCA+AGG - chr5_3:27328445-27328464 Msa0806840:intron 25.0%
! TGTTTGTTCTACAAAAACAA+AGG + chr5_3:27328765-27328784 None:intergenic 25.0%
! GAATGATCATAAATCAAAGA+AGG + chr5_3:27328915-27328934 None:intergenic 25.0%
!!! AATTACTTTTACAACTACCT+AGG + chr5_3:27329158-27329177 None:intergenic 25.0%
!! ACATTTTAGTAGAATCGGTA+CGG + chr5_3:27327707-27327726 None:intergenic 30.0%
!! TACCGATTCTACTAAAATGT+CGG - chr5_3:27327707-27327726 Msa0806840:CDS 30.0%
TGTATATCTAAGAACCAATG+TGG - chr5_3:27327754-27327773 Msa0806840:intron 30.0%
!! AGGTATTTTTATCACCACAT+TGG + chr5_3:27327771-27327790 None:intergenic 30.0%
GGTTCATCTAATACTTTGAA+TGG + chr5_3:27327976-27327995 None:intergenic 30.0%
AGTATTAGATGAACCTATTG+AGG - chr5_3:27327981-27328000 Msa0806840:intron 30.0%
AAACTTGATGTTATGTGTTC+AGG - chr5_3:27328028-27328047 Msa0806840:intron 30.0%
AACTTGATGTTATGTGTTCA+GGG - chr5_3:27328029-27328048 Msa0806840:intron 30.0%
GATGATACATACACACTTAA+AGG - chr5_3:27328074-27328093 Msa0806840:intron 30.0%
!! TTTCAAAGCCTTTTAAAGAG+TGG + chr5_3:27328124-27328143 None:intergenic 30.0%
!!! TCCTTTGATTTTTTGTAGGT+TGG - chr5_3:27328316-27328335 Msa0806840:intron 30.0%
ACCAACCTACAAAAAATCAA+AGG + chr5_3:27328320-27328339 None:intergenic 30.0%
!!! TTTGATTTTTTGTAGGTTGG+TGG - chr5_3:27328319-27328338 Msa0806840:intron 30.0%
ATTTGGGAATGATAGAAACA+AGG + chr5_3:27328435-27328454 None:intergenic 30.0%
AGGCCTTGATAAAGAAATTT+GGG + chr5_3:27328451-27328470 None:intergenic 30.0%
TAGGCCTTGATAAAGAAATT+TGG + chr5_3:27328452-27328471 None:intergenic 30.0%
AATAAGAAACATACCGACAA+GGG + chr5_3:27328494-27328513 None:intergenic 30.0%
TAATAAGAAACATACCGACA+AGG + chr5_3:27328495-27328514 None:intergenic 30.0%
! ATATTGTGGACTGTTTAGAT+AGG - chr5_3:27328559-27328578 Msa0806840:CDS 30.0%
!!! TTCGTGTGATTTTCAAGTAT+TGG + chr5_3:27328599-27328618 None:intergenic 30.0%
! TTTTCAAACTAATAGCGATC+AGG + chr5_3:27328692-27328711 None:intergenic 30.0%
! TCACTGAGTTTCTATTTTCA+AGG - chr5_3:27328825-27328844 Msa0806840:CDS 30.0%
! CACTGAGTTTCTATTTTCAA+GGG - chr5_3:27328826-27328845 Msa0806840:CDS 30.0%
!! ACTGAGTTTCTATTTTCAAG+GGG - chr5_3:27328827-27328846 Msa0806840:CDS 30.0%
AATTACACAAAACACACCAA+TGG + chr5_3:27328865-27328884 None:intergenic 30.0%
TGATCATAAATCAAAGAAGG+CGG + chr5_3:27328912-27328931 None:intergenic 30.0%
TGTACAAAACAATCTGTGTA+AGG + chr5_3:27328967-27328986 None:intergenic 30.0%
!! AGTTATTGACTTTCATTGCA+AGG + chr5_3:27329118-27329137 None:intergenic 30.0%
TTGCAATGAAAGTCAATAAC+TGG - chr5_3:27329117-27329136 Msa0806840:intron 30.0%
AATTTATATCGCACGCAATA+AGG + chr5_3:27329190-27329209 None:intergenic 30.0%
AAATCTATTTGTCCACCAAT+TGG - chr5_3:27329344-27329363 Msa0806840:intron 30.0%
AATCTATTTGTCCACCAATT+GGG - chr5_3:27329345-27329364 Msa0806840:intron 30.0%
AGACAATAACATAACAGCAA+TGG - chr5_3:27329373-27329392 Msa0806840:intron 30.0%
AAAGAATTGTCGAAGAGATT+AGG + chr5_3:27329410-27329429 None:intergenic 30.0%
TCTGATTCAATGGTCAAAAT+GGG - chr5_3:27329563-27329582 Msa0806840:CDS 30.0%
!! ACATTTTAGTAGAATCGGTA+CGG + chr5_3:27327707-27327726 None:intergenic 30.0%
!! TACCGATTCTACTAAAATGT+CGG - chr5_3:27327707-27327726 Msa0806840:CDS 30.0%
TGTATATCTAAGAACCAATG+TGG - chr5_3:27327754-27327773 Msa0806840:intron 30.0%
!! AGGTATTTTTATCACCACAT+TGG + chr5_3:27327771-27327790 None:intergenic 30.0%
GGTTCATCTAATACTTTGAA+TGG + chr5_3:27327976-27327995 None:intergenic 30.0%
AGTATTAGATGAACCTATTG+AGG - chr5_3:27327981-27328000 Msa0806840:intron 30.0%
AAACTTGATGTTATGTGTTC+AGG - chr5_3:27328028-27328047 Msa0806840:intron 30.0%
AACTTGATGTTATGTGTTCA+GGG - chr5_3:27328029-27328048 Msa0806840:intron 30.0%
GATGATACATACACACTTAA+AGG - chr5_3:27328074-27328093 Msa0806840:intron 30.0%
!! TTTCAAAGCCTTTTAAAGAG+TGG + chr5_3:27328124-27328143 None:intergenic 30.0%
!!! TCCTTTGATTTTTTGTAGGT+TGG - chr5_3:27328316-27328335 Msa0806840:intron 30.0%
ACCAACCTACAAAAAATCAA+AGG + chr5_3:27328320-27328339 None:intergenic 30.0%
!!! TTTGATTTTTTGTAGGTTGG+TGG - chr5_3:27328319-27328338 Msa0806840:intron 30.0%
ATTTGGGAATGATAGAAACA+AGG + chr5_3:27328435-27328454 None:intergenic 30.0%
AGGCCTTGATAAAGAAATTT+GGG + chr5_3:27328451-27328470 None:intergenic 30.0%
TAGGCCTTGATAAAGAAATT+TGG + chr5_3:27328452-27328471 None:intergenic 30.0%
AATAAGAAACATACCGACAA+GGG + chr5_3:27328494-27328513 None:intergenic 30.0%
TAATAAGAAACATACCGACA+AGG + chr5_3:27328495-27328514 None:intergenic 30.0%
! ATATTGTGGACTGTTTAGAT+AGG - chr5_3:27328559-27328578 Msa0806840:CDS 30.0%
!!! TTCGTGTGATTTTCAAGTAT+TGG + chr5_3:27328599-27328618 None:intergenic 30.0%
! TTTTCAAACTAATAGCGATC+AGG + chr5_3:27328692-27328711 None:intergenic 30.0%
! TCACTGAGTTTCTATTTTCA+AGG - chr5_3:27328825-27328844 Msa0806840:CDS 30.0%
! CACTGAGTTTCTATTTTCAA+GGG - chr5_3:27328826-27328845 Msa0806840:CDS 30.0%
!! ACTGAGTTTCTATTTTCAAG+GGG - chr5_3:27328827-27328846 Msa0806840:CDS 30.0%
AATTACACAAAACACACCAA+TGG + chr5_3:27328865-27328884 None:intergenic 30.0%
TGATCATAAATCAAAGAAGG+CGG + chr5_3:27328912-27328931 None:intergenic 30.0%
TGTACAAAACAATCTGTGTA+AGG + chr5_3:27328967-27328986 None:intergenic 30.0%
!! AGTTATTGACTTTCATTGCA+AGG + chr5_3:27329118-27329137 None:intergenic 30.0%
TTGCAATGAAAGTCAATAAC+TGG - chr5_3:27329117-27329136 Msa0806840:intron 30.0%
AATTTATATCGCACGCAATA+AGG + chr5_3:27329190-27329209 None:intergenic 30.0%
AAATCTATTTGTCCACCAAT+TGG - chr5_3:27329344-27329363 Msa0806840:intron 30.0%
AATCTATTTGTCCACCAATT+GGG - chr5_3:27329345-27329364 Msa0806840:intron 30.0%
AGACAATAACATAACAGCAA+TGG - chr5_3:27329373-27329392 Msa0806840:intron 30.0%
AAAGAATTGTCGAAGAGATT+AGG + chr5_3:27329410-27329429 None:intergenic 30.0%
TCTGATTCAATGGTCAAAAT+GGG - chr5_3:27329563-27329582 Msa0806840:CDS 30.0%
!!! GTTCTTTTGTTTGGCTTTCT+TGG - chr5_3:27327409-27327428 Msa0806840:CDS 35.0%
TAGTGATCAAGTGTTAGTTG+AGG + chr5_3:27327460-27327479 None:intergenic 35.0%
CTAACAATGTCAAACACAGT+AGG + chr5_3:27327496-27327515 None:intergenic 35.0%
CTACTGTGTTTGACATTGTT+AGG - chr5_3:27327494-27327513 Msa0806840:CDS 35.0%
TGACATTGTTAGGAAGCAAA+TGG - chr5_3:27327504-27327523 Msa0806840:CDS 35.0%
TAGGAAGCAAATGGAATGTT+CGG - chr5_3:27327513-27327532 Msa0806840:CDS 35.0%
!! GACCGACATTTTAGTAGAAT+CGG + chr5_3:27327712-27327731 None:intergenic 35.0%
AAAATGTCGGTCATCATCTT+CGG - chr5_3:27327720-27327739 Msa0806840:CDS 35.0%
ACCAACATCCACTCTTTAAA+AGG - chr5_3:27328113-27328132 Msa0806840:intron 35.0%
TGCTGATATTCTCACAATTG+CGG - chr5_3:27328199-27328218 Msa0806840:intron 35.0%
ATAGAAACAAGGCTTTCATC+AGG + chr5_3:27328424-27328443 None:intergenic 35.0%
! GGAGAAGTGTATCTTATCAT+AGG - chr5_3:27328530-27328549 Msa0806840:CDS 35.0%
AATCACACGAAACTACAACA+AGG - chr5_3:27328608-27328627 Msa0806840:CDS 35.0%
TTGTAGAACAAACATCCATC+AGG - chr5_3:27328771-27328790 Msa0806840:CDS 35.0%
GTTCTCATTCATATGTGAGA+CGG + chr5_3:27328800-27328819 None:intergenic 35.0%
CTCACATATGAATGAGAACA+AGG - chr5_3:27328801-27328820 Msa0806840:CDS 35.0%
GATCATAAATCAAAGAAGGC+GGG + chr5_3:27328911-27328930 None:intergenic 35.0%
TACCACAATAATCCTCATCT+CGG - chr5_3:27329007-27329026 Msa0806840:intron 35.0%
AACTACCTAGGAATCTTATG+AGG + chr5_3:27329146-27329165 None:intergenic 35.0%
! TTGAACTGATTGTCTGAGAT+TGG + chr5_3:27329317-27329336 None:intergenic 35.0%
! TTGGTGGACAAATAGATTTC+AGG + chr5_3:27329343-27329362 None:intergenic 35.0%
ATCTATTTGTCCACCAATTG+GGG - chr5_3:27329346-27329365 Msa0806840:intron 35.0%
TCAGTTACTATTAAAAGGCG+AGG - chr5_3:27329433-27329452 Msa0806840:CDS 35.0%
CAGTTACTATTAAAAGGCGA+GGG - chr5_3:27329434-27329453 Msa0806840:CDS 35.0%
CTCTGATTCAATGGTCAAAA+TGG - chr5_3:27329562-27329581 Msa0806840:CDS 35.0%
AATTCAGAAAGCTTCATCAC+TGG - chr5_3:27329596-27329615 Msa0806840:CDS 35.0%
!!! GTTCTTTTGTTTGGCTTTCT+TGG - chr5_3:27327409-27327428 Msa0806840:CDS 35.0%
TAGTGATCAAGTGTTAGTTG+AGG + chr5_3:27327460-27327479 None:intergenic 35.0%
CTAACAATGTCAAACACAGT+AGG + chr5_3:27327496-27327515 None:intergenic 35.0%
CTACTGTGTTTGACATTGTT+AGG - chr5_3:27327494-27327513 Msa0806840:CDS 35.0%
TGACATTGTTAGGAAGCAAA+TGG - chr5_3:27327504-27327523 Msa0806840:CDS 35.0%
TAGGAAGCAAATGGAATGTT+CGG - chr5_3:27327513-27327532 Msa0806840:CDS 35.0%
!! GACCGACATTTTAGTAGAAT+CGG + chr5_3:27327712-27327731 None:intergenic 35.0%
AAAATGTCGGTCATCATCTT+CGG - chr5_3:27327720-27327739 Msa0806840:CDS 35.0%
ACCAACATCCACTCTTTAAA+AGG - chr5_3:27328113-27328132 Msa0806840:intron 35.0%
TGCTGATATTCTCACAATTG+CGG - chr5_3:27328199-27328218 Msa0806840:intron 35.0%
ATAGAAACAAGGCTTTCATC+AGG + chr5_3:27328424-27328443 None:intergenic 35.0%
! GGAGAAGTGTATCTTATCAT+AGG - chr5_3:27328530-27328549 Msa0806840:CDS 35.0%
AATCACACGAAACTACAACA+AGG - chr5_3:27328608-27328627 Msa0806840:CDS 35.0%
TTGTAGAACAAACATCCATC+AGG - chr5_3:27328771-27328790 Msa0806840:CDS 35.0%
GTTCTCATTCATATGTGAGA+CGG + chr5_3:27328800-27328819 None:intergenic 35.0%
CTCACATATGAATGAGAACA+AGG - chr5_3:27328801-27328820 Msa0806840:CDS 35.0%
GATCATAAATCAAAGAAGGC+GGG + chr5_3:27328911-27328930 None:intergenic 35.0%
TACCACAATAATCCTCATCT+CGG - chr5_3:27329007-27329026 Msa0806840:intron 35.0%
AACTACCTAGGAATCTTATG+AGG + chr5_3:27329146-27329165 None:intergenic 35.0%
! TTGAACTGATTGTCTGAGAT+TGG + chr5_3:27329317-27329336 None:intergenic 35.0%
! TTGGTGGACAAATAGATTTC+AGG + chr5_3:27329343-27329362 None:intergenic 35.0%
ATCTATTTGTCCACCAATTG+GGG - chr5_3:27329346-27329365 Msa0806840:intron 35.0%
TCAGTTACTATTAAAAGGCG+AGG - chr5_3:27329433-27329452 Msa0806840:CDS 35.0%
CAGTTACTATTAAAAGGCGA+GGG - chr5_3:27329434-27329453 Msa0806840:CDS 35.0%
CTCTGATTCAATGGTCAAAA+TGG - chr5_3:27329562-27329581 Msa0806840:CDS 35.0%
AATTCAGAAAGCTTCATCAC+TGG - chr5_3:27329596-27329615 Msa0806840:CDS 35.0%
! CTTCCATGACTGCTTTATTC+AGG - chr5_3:27327579-27327598 Msa0806840:CDS 40.0%
AGACCTGAATAAAGCAGTCA+TGG + chr5_3:27327585-27327604 None:intergenic 40.0%
AGTAGAATCGGTACGGTAAA+TGG + chr5_3:27327700-27327719 None:intergenic 40.0%
!! GCCTTTTAAAGAGTGGATGT+TGG + chr5_3:27328117-27328136 None:intergenic 40.0%
!! TTCATCAGGTGTTGGAAGAT+TGG + chr5_3:27328410-27328429 None:intergenic 40.0%
TTATCAAGGCCTATCAGTCA+CGG - chr5_3:27328459-27328478 Msa0806840:intron 40.0%
! TTGTGGACTGTTTAGATAGG+TGG - chr5_3:27328562-27328581 Msa0806840:CDS 40.0%
! CAGAGGACTATGAGCTTTTT+CGG + chr5_3:27328648-27328667 None:intergenic 40.0%
TGTCTCACTCATTAAGACAG+AGG + chr5_3:27328665-27328684 None:intergenic 40.0%
!! GAGTTTCTATTTTCAAGGGG+CGG - chr5_3:27328830-27328849 Msa0806840:CDS 40.0%
ATTTCATTTGAGCCGAGATG+AGG + chr5_3:27329022-27329041 None:intergenic 40.0%
TGCAAGGCAATGTGAAGAAA+TGG + chr5_3:27329102-27329121 None:intergenic 40.0%
GGTTTCCTCATAAGATTCCT+AGG - chr5_3:27329138-27329157 Msa0806840:intron 40.0%
TTTCTGATCACAACACATGC+AGG - chr5_3:27329209-27329228 Msa0806840:intron 40.0%
TCTATTTGTCCACCAATTGG+GGG - chr5_3:27329347-27329366 Msa0806840:intron 40.0%
GAAATGTACTCGAGTGTGTT+CGG - chr5_3:27329476-27329495 Msa0806840:CDS 40.0%
CCATTGAATCAGAGAATTGC+TGG + chr5_3:27329556-27329575 None:intergenic 40.0%
CCAGCAATTCTCTGATTCAA+TGG - chr5_3:27329553-27329572 Msa0806840:CDS 40.0%
GCTTCATCACTGGAGAAATT+AGG - chr5_3:27329606-27329625 Msa0806840:exon 40.0%
! CTTCCATGACTGCTTTATTC+AGG - chr5_3:27327579-27327598 Msa0806840:CDS 40.0%
AGACCTGAATAAAGCAGTCA+TGG + chr5_3:27327585-27327604 None:intergenic 40.0%
AGTAGAATCGGTACGGTAAA+TGG + chr5_3:27327700-27327719 None:intergenic 40.0%
!! GCCTTTTAAAGAGTGGATGT+TGG + chr5_3:27328117-27328136 None:intergenic 40.0%
!! TTCATCAGGTGTTGGAAGAT+TGG + chr5_3:27328410-27328429 None:intergenic 40.0%
TTATCAAGGCCTATCAGTCA+CGG - chr5_3:27328459-27328478 Msa0806840:intron 40.0%
! TTGTGGACTGTTTAGATAGG+TGG - chr5_3:27328562-27328581 Msa0806840:CDS 40.0%
! CAGAGGACTATGAGCTTTTT+CGG + chr5_3:27328648-27328667 None:intergenic 40.0%
TGTCTCACTCATTAAGACAG+AGG + chr5_3:27328665-27328684 None:intergenic 40.0%
!! GAGTTTCTATTTTCAAGGGG+CGG - chr5_3:27328830-27328849 Msa0806840:CDS 40.0%
ATTTCATTTGAGCCGAGATG+AGG + chr5_3:27329022-27329041 None:intergenic 40.0%
TGCAAGGCAATGTGAAGAAA+TGG + chr5_3:27329102-27329121 None:intergenic 40.0%
GGTTTCCTCATAAGATTCCT+AGG - chr5_3:27329138-27329157 Msa0806840:intron 40.0%
TTTCTGATCACAACACATGC+AGG - chr5_3:27329209-27329228 Msa0806840:intron 40.0%
TCTATTTGTCCACCAATTGG+GGG - chr5_3:27329347-27329366 Msa0806840:intron 40.0%
GAAATGTACTCGAGTGTGTT+CGG - chr5_3:27329476-27329495 Msa0806840:CDS 40.0%
CCATTGAATCAGAGAATTGC+TGG + chr5_3:27329556-27329575 None:intergenic 40.0%
CCAGCAATTCTCTGATTCAA+TGG - chr5_3:27329553-27329572 Msa0806840:CDS 40.0%
GCTTCATCACTGGAGAAATT+AGG - chr5_3:27329606-27329625 Msa0806840:exon 40.0%
TTATGTGTTCAGGGTTGCGA+TGG - chr5_3:27328038-27328057 Msa0806840:intron 45.0%
! TTTGTTGAATCAGAGTGCCC+TGG - chr5_3:27328164-27328183 Msa0806840:intron 45.0%
CAGCACAAGAAACAACTCCA+GGG + chr5_3:27328184-27328203 None:intergenic 45.0%
TCAGCACAAGAAACAACTCC+AGG + chr5_3:27328185-27328204 None:intergenic 45.0%
GGCTAGAGATGCTGTAATTC+TGG - chr5_3:27328220-27328239 Msa0806840:intron 45.0%
!! TGTAGGTTGGTGGACCATAT+TGG - chr5_3:27328329-27328348 Msa0806840:intron 45.0%
!! GTAGGTTGGTGGACCATATT+GGG - chr5_3:27328330-27328349 Msa0806840:intron 45.0%
ACCATATTGGGATGTTCCTG+TGG - chr5_3:27328342-27328361 Msa0806840:intron 45.0%
CCATATTGGGATGTTCCTGT+GGG - chr5_3:27328343-27328362 Msa0806840:intron 45.0%
ACAGAATCTTTCCTTCCCAC+AGG + chr5_3:27328361-27328380 None:intergenic 45.0%
!! ATCAGGTGTTGGAAGATTGG+TGG + chr5_3:27328407-27328426 None:intergenic 45.0%
ACAAGGCTTTCATCAGGTGT+TGG + chr5_3:27328418-27328437 None:intergenic 45.0%
CAACCATATCCGTGACTGAT+AGG + chr5_3:27328471-27328490 None:intergenic 45.0%
AGCCGAGATGAGGATTATTG+TGG + chr5_3:27329012-27329031 None:intergenic 45.0%
TTGACACCTTGCCATACCAA+TGG + chr5_3:27329243-27329262 None:intergenic 45.0%
TTATTGTCTCCTCCCCCAAT+TGG + chr5_3:27329362-27329381 None:intergenic 45.0%
TTATGTGTTCAGGGTTGCGA+TGG - chr5_3:27328038-27328057 Msa0806840:intron 45.0%
! TTTGTTGAATCAGAGTGCCC+TGG - chr5_3:27328164-27328183 Msa0806840:intron 45.0%
CAGCACAAGAAACAACTCCA+GGG + chr5_3:27328184-27328203 None:intergenic 45.0%
TCAGCACAAGAAACAACTCC+AGG + chr5_3:27328185-27328204 None:intergenic 45.0%
GGCTAGAGATGCTGTAATTC+TGG - chr5_3:27328220-27328239 Msa0806840:intron 45.0%
!! TGTAGGTTGGTGGACCATAT+TGG - chr5_3:27328329-27328348 Msa0806840:intron 45.0%
!! GTAGGTTGGTGGACCATATT+GGG - chr5_3:27328330-27328349 Msa0806840:intron 45.0%
ACCATATTGGGATGTTCCTG+TGG - chr5_3:27328342-27328361 Msa0806840:intron 45.0%
CCATATTGGGATGTTCCTGT+GGG - chr5_3:27328343-27328362 Msa0806840:intron 45.0%
ACAGAATCTTTCCTTCCCAC+AGG + chr5_3:27328361-27328380 None:intergenic 45.0%
!! ATCAGGTGTTGGAAGATTGG+TGG + chr5_3:27328407-27328426 None:intergenic 45.0%
ACAAGGCTTTCATCAGGTGT+TGG + chr5_3:27328418-27328437 None:intergenic 45.0%
CAACCATATCCGTGACTGAT+AGG + chr5_3:27328471-27328490 None:intergenic 45.0%
AGCCGAGATGAGGATTATTG+TGG + chr5_3:27329012-27329031 None:intergenic 45.0%
TTGACACCTTGCCATACCAA+TGG + chr5_3:27329243-27329262 None:intergenic 45.0%
TTATTGTCTCCTCCCCCAAT+TGG + chr5_3:27329362-27329381 None:intergenic 45.0%
TGTTCAGGGTTGCGATGGAT+CGG - chr5_3:27328043-27328062 Msa0806840:intron 50.0%
CCCACAGGAACATCCCAATA+TGG + chr5_3:27328346-27328365 None:intergenic 50.0%
ATTGGGATGTTCCTGTGGGA+AGG - chr5_3:27328347-27328366 Msa0806840:intron 50.0%
AGGCCTATCAGTCACGGATA+TGG - chr5_3:27328465-27328484 Msa0806840:intron 50.0%
ACGGATATGGTTGCCCTTGT+CGG - chr5_3:27328478-27328497 Msa0806840:intron 50.0%
TATGTGAGACGGTGACCTGA+TGG + chr5_3:27328789-27328808 None:intergenic 50.0%
AGGATCCCACACCATTGGTA+TGG - chr5_3:27329229-27329248 Msa0806840:intron 50.0%
CCTTGCCATACCAATGGTGT+GGG + chr5_3:27329237-27329256 None:intergenic 50.0%
ACCTTGCCATACCAATGGTG+TGG + chr5_3:27329238-27329257 None:intergenic 50.0%
ATTTGTCCACCAATTGGGGG+AGG - chr5_3:27329350-27329369 Msa0806840:intron 50.0%
TGTTCAGGGTTGCGATGGAT+CGG - chr5_3:27328043-27328062 Msa0806840:intron 50.0%
CCCACAGGAACATCCCAATA+TGG + chr5_3:27328346-27328365 None:intergenic 50.0%
ATTGGGATGTTCCTGTGGGA+AGG - chr5_3:27328347-27328366 Msa0806840:intron 50.0%
AGGCCTATCAGTCACGGATA+TGG - chr5_3:27328465-27328484 Msa0806840:intron 50.0%
ACGGATATGGTTGCCCTTGT+CGG - chr5_3:27328478-27328497 Msa0806840:intron 50.0%
TATGTGAGACGGTGACCTGA+TGG + chr5_3:27328789-27328808 None:intergenic 50.0%
AGGATCCCACACCATTGGTA+TGG - chr5_3:27329229-27329248 Msa0806840:intron 50.0%
CCTTGCCATACCAATGGTGT+GGG + chr5_3:27329237-27329256 None:intergenic 50.0%
ACCTTGCCATACCAATGGTG+TGG + chr5_3:27329238-27329257 None:intergenic 50.0%
ATTTGTCCACCAATTGGGGG+AGG - chr5_3:27329350-27329369 Msa0806840:intron 50.0%
TGAACCTCGTAACGCAGCCT+CGG - chr5_3:27327543-27327562 Msa0806840:CDS 55.0%
GTAACGCAGCCTCGGTTGTT+CGG - chr5_3:27327551-27327570 Msa0806840:CDS 55.0%
GAAGTGAAGCCGAACAACCG+AGG + chr5_3:27327563-27327582 None:intergenic 55.0%
GGGGCGGCATTATTCACCAT+TGG - chr5_3:27328846-27328865 Msa0806840:CDS 55.0%
CATGCAGGATCCCACACCAT+TGG - chr5_3:27329224-27329243 Msa0806840:intron 55.0%
CCCACACCATTGGTATGGCA+AGG - chr5_3:27329234-27329253 Msa0806840:intron 55.0%
TTGTCTCCTCCCCCAATTGG+TGG + chr5_3:27329359-27329378 None:intergenic 55.0%
TGAACCTCGTAACGCAGCCT+CGG - chr5_3:27327543-27327562 Msa0806840:CDS 55.0%
GTAACGCAGCCTCGGTTGTT+CGG - chr5_3:27327551-27327570 Msa0806840:CDS 55.0%
GAAGTGAAGCCGAACAACCG+AGG + chr5_3:27327563-27327582 None:intergenic 55.0%
GGGGCGGCATTATTCACCAT+TGG - chr5_3:27328846-27328865 Msa0806840:CDS 55.0%
CATGCAGGATCCCACACCAT+TGG - chr5_3:27329224-27329243 Msa0806840:intron 55.0%
CCCACACCATTGGTATGGCA+AGG - chr5_3:27329234-27329253 Msa0806840:intron 55.0%
TTGTCTCCTCCCCCAATTGG+TGG + chr5_3:27329359-27329378 None:intergenic 55.0%
ACAACCGAGGCTGCGTTACG+AGG + chr5_3:27327550-27327569 None:intergenic 60.0%
ACAACCGAGGCTGCGTTACG+AGG + chr5_3:27327550-27327569 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 27327328 27329655 27327328 ID=Msa0806840;Name=Msa0806840
chr5_3 mRNA 27327328 27329655 27327328 ID=Msa0806840-mRNA-1;Parent=Msa0806840;Name=Msa0806840-mRNA-1;_AED=0.09;_eAED=0.09;_QI=39|1|0.75|1|1|1|4|0|338
chr5_3 exon 27327328 27327752 27327328 ID=Msa0806840-mRNA-1:exon:9711;Parent=Msa0806840-mRNA-1
chr5_3 exon 27328484 27328649 27328484 ID=Msa0806840-mRNA-1:exon:9710;Parent=Msa0806840-mRNA-1
chr5_3 exon 27328742 27328933 27328742 ID=Msa0806840-mRNA-1:exon:9709;Parent=Msa0806840-mRNA-1
chr5_3 exon 27329383 27329655 27329383 ID=Msa0806840-mRNA-1:exon:9708;Parent=Msa0806840-mRNA-1
chr5_3 five_prime_UTR 27329617 27329655 27329617 ID=Msa0806840-mRNA-1:five_prime_utr;Parent=Msa0806840-mRNA-1
chr5_3 CDS 27329383 27329616 27329383 ID=Msa0806840-mRNA-1:cds;Parent=Msa0806840-mRNA-1
chr5_3 CDS 27328742 27328933 27328742 ID=Msa0806840-mRNA-1:cds;Parent=Msa0806840-mRNA-1
chr5_3 CDS 27328484 27328649 27328484 ID=Msa0806840-mRNA-1:cds;Parent=Msa0806840-mRNA-1
chr5_3 CDS 27327328 27327752 27327328 ID=Msa0806840-mRNA-1:cds;Parent=Msa0806840-mRNA-1
Gene Sequence

>Msa0806840

ATGGATACTCCATTTCTGTCAAAATTTGTTTTTTTACATATTGTTCTTTTGTTTGGCTTTCTTGGTGACAATAGATTGTATGCAAGTGAGCCTCAACTAACACTTGATCACTATGCATCAACTTGTCCTACTGTGTTTGACATTGTTAGGAAGCAAATGGAATGTTCGGTGCTTTCTGAACCTCGTAACGCAGCCTCGGTTGTTCGGCTTCACTTCCATGACTGCTTTATTCAGGGTTGCGATGGATCGGTCTTGCTCGATGATACATACACACTTAAAGGTGAAAAGAAAGCAGCTACCAACATCCACTCTTTAAAAGGCTTTGAAATTATCGATCAAATCAAGAACTTTGTTGAATCAGAGTGCCCTGGAGTTGTTTCTTGTGCTGATATTCTCACAATTGCGGCTAGAGATGCTGTAATTCTGGTTGGTGGACCATATTGGGATGTTCCTGTGGGAAGGAAAGATTCTGTGACTGCAAGTTTTGAACTTGCTACCACCAATCTTCCAACACCTGATGAAAGCCTTGTTTCTATCATTCCCAAATTTCTTTATCAAGGCCTATCAGTCACGGATATGGTTGCCCTTGTCGGATCCCACACCATTGGTATGGCAAGGTGTCAAAATTTTCGATCAAGAATATATGGAGACTATGAATCAACTTCAGTGAAAAATCCAATCTCAGACAATCAGTTCAACAACCTGAAATCTATTTGTCCACCAATTGGGGGAGGAGACAATAACATAACAGCAATGGACTATGTGACGCCTAATCTCTTCGACAATTCTTTCTATCAGTTACTATTAAAAGGCGAGGGTGTTCTTAACTCAGATCAAGAAATGTACTCGAGTGTGTTCGGAATCGAAACAAGAGAGCTTGTCAAGAAGTATGCAGTAGACTCTCTTGCTTTCTTCCAGCAATTCTCTGATTCAATGGTCAAAATGGGAAATATCACAAATTCAGAAAGCTTCATCACTGGAGAAATTAGGAAGAACTGCAGATTTGTTAACACGTAA
Protein sequence

>Msa0806840

MDTPFLSKFVFLHIVLLFGFLGDNRLYASEPQLTLDHYASTCPTVFDIVRKQMECSVLSEPRNAASVVRLHFHDCFIQGCDGSVLLDDTYTLKGEKKAATNIHSLKGFEIIDQIKNFVESECPGVVSCADILTIAARDAVILVGGPYWDVPVGRKDSVTASFELATTNLPTPDESLVSIIPKFLYQGLSVTDMVALVGSHTIGMARCQNFRSRIYGDYESTSVKNPISDNQFNNLKSICPPIGGGDNNITAMDYVTPNLFDNSFYQLLLKGEGVLNSDQEMYSSVFGIETRELVKKYAVDSLAFFQQFSDSMVKMGNITNSESFITGEIRKNCRFVNT*