AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1054250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1054250 MtrunA17_Chr7g0224391 99.085 437 4 0 1 437 1 437 0.0 900
Msa1054250 MtrunA17_Chr3g0132001 66.587 419 137 2 20 437 18 434 0.0 587
Msa1054250 MtrunA17_Chr8g0386141 61.682 428 159 3 12 437 4 428 0.0 540
Msa1054250 MtrunA17_Chr4g0001861 34.483 435 266 7 13 435 3 430 3.12e-85 267
Msa1054250 MtrunA17_Chr2g0334001 34.247 438 262 9 13 435 3 429 7.08e-83 261
Msa1054250 MtrunA17_Chr8g0371821 34.597 422 262 8 23 435 10 426 1.75e-79 252
Msa1054250 MtrunA17_Chr5g0433641 35.659 387 235 8 20 397 14 395 1.09e-76 246
Msa1054250 MtrunA17_Chr1g0191081 34.651 430 254 11 19 431 21 440 3.61e-76 244
Msa1054250 MtrunA17_Chr1g0165671 34.635 384 239 7 19 396 30 407 2.63e-75 243
Msa1054250 MtrunA17_Chr2g0306321 36.364 396 228 15 36 412 41 431 5.30e-74 239
Msa1054250 MtrunA17_Chr1g0165661 33.570 423 263 10 20 429 13 430 8.17e-73 236
Msa1054250 MtrunA17_Chr7g0273721 34.367 387 239 8 19 395 14 395 1.13e-72 236
Msa1054250 MtrunA17_Chr1g0165681 33.858 381 240 8 23 396 16 391 8.42e-72 233
Msa1054250 MtrunA17_Chr7g0273761 32.639 432 265 10 20 433 386 809 1.61e-69 235
Msa1054250 MtrunA17_Chr7g0273761 30.612 294 190 6 150 433 32 321 5.60e-39 149
Msa1054250 MtrunA17_Chr7g0273781 32.727 385 244 6 20 395 47 425 3.20e-68 225
Msa1054250 MtrunA17_Chr7g0273731 32.812 384 247 6 19 395 96 475 4.11e-67 223
Msa1054250 MtrunA17_Chr7g0273791 31.593 383 251 6 20 395 14 392 2.09e-65 217
Msa1054250 MtrunA17_Chr3g0141851 32.483 431 260 13 22 436 18 433 7.43e-65 215
Msa1054250 MtrunA17_Chr2g0311401 36.455 299 170 5 150 434 6 298 1.55e-64 209
Msa1054250 MtrunA17_Chr3g0087401 30.769 442 266 12 24 432 10 444 1.01e-63 212
Msa1054250 MtrunA17_Chr7g0235531 31.019 432 278 9 20 435 8 435 5.29e-61 204
Msa1054250 MtrunA17_Chr7g0252731 29.736 417 259 10 22 426 23 417 2.77e-60 202
Msa1054250 MtrunA17_Chr4g0027071 32.063 446 270 15 18 436 14 453 3.39e-60 203
Msa1054250 MtrunA17_Chr4g0072041 32.750 400 238 9 24 396 22 417 1.90e-58 198
Msa1054250 MtrunA17_Chr1g0165711 32.283 381 211 10 21 396 14 352 3.37e-56 191
Msa1054250 MtrunA17_Chr3g0113821 31.481 432 274 11 20 436 9 433 9.92e-56 190
Msa1054250 MtrunA17_Chr4g0054081 30.025 403 243 9 24 395 17 411 1.23e-49 175
Msa1054250 MtrunA17_Chr7g0253541 30.072 419 241 16 13 391 2 408 2.15e-46 166
Msa1054250 MtrunA17_Chr2g0283361 29.298 413 273 12 39 437 29 436 1.07e-44 161
Msa1054250 MtrunA17_Chr6g0470591 31.903 373 228 12 38 392 42 406 2.45e-43 158
Msa1054250 MtrunA17_Chr3g0106691 32.000 400 228 15 23 392 63 448 5.40e-43 158
Msa1054250 MtrunA17_Chr7g0253531 28.385 384 233 10 51 396 37 416 3.12e-39 147
Msa1054250 MtrunA17_Chr7g0253501 28.283 396 218 12 51 396 37 416 1.17e-38 145
Msa1054250 MtrunA17_Chr3g0106721 34.091 308 185 11 65 360 65 366 1.42e-38 145
Msa1054250 MtrunA17_Chr3g0106661 28.000 450 284 16 23 436 17 462 1.14e-37 142
Msa1054250 MtrunA17_Chr7g0270771 34.643 280 151 10 23 294 12 267 1.53e-37 137
Msa1054250 MtrunA17_Chr3g0106761 31.845 336 188 15 82 392 28 347 1.32e-35 135
Msa1054250 MtrunA17_Chr3g0123061 27.114 402 246 15 23 408 12 382 1.17e-34 133
Msa1054250 MtrunA17_Chr3g0106741 32.143 336 187 17 82 392 59 378 1.30e-33 130
Msa1054250 MtrunA17_Chr5g0442691 28.133 391 239 13 37 393 54 436 2.33e-33 131
Msa1054250 MtrunA17_Chr5g0442701 27.877 391 240 13 37 393 31 413 1.67e-32 128
Msa1054250 MtrunA17_Chr6g0471971 31.890 254 130 9 165 408 1 221 1.95e-32 122
Msa1054250 MtrunA17_Chr7g0252771 27.344 256 172 4 189 435 1 251 1.41e-30 118
Msa1054250 MtrunA17_Chr3g0134091 30.476 315 193 10 56 356 56 358 2.39e-30 122
Msa1054250 MtrunA17_Chr3g0134071 27.340 406 250 16 55 432 57 445 2.97e-28 116
Msa1054250 MtrunA17_Chr4g0053061 27.228 404 247 17 58 429 77 465 3.56e-27 113
Msa1054250 MtrunA17_Chr3g0106711 37.037 189 105 5 23 199 17 203 6.25e-27 108
Msa1054250 MtrunA17_Chr5g0442721 25.355 422 268 15 9 393 4 415 9.06e-27 112
Msa1054250 MtrunA17_Chr8g0359251 25.707 389 258 10 63 433 58 433 1.62e-26 110
Msa1054250 MtrunA17_Chr3g0134041 25.310 403 259 12 55 432 53 438 1.66e-26 110
Msa1054250 MtrunA17_Chr6g0471381 30.653 199 128 6 201 395 7 199 1.75e-26 107
Msa1054250 MtrunA17_Chr3g0134121 28.610 367 226 13 14 356 5 359 2.15e-26 111
Msa1054250 MtrunA17_Chr4g0053111 26.733 404 254 14 54 429 46 435 2.58e-26 110
Msa1054250 MtrunA17_Chr8g0374911 26.000 400 257 16 58 429 70 458 3.26e-26 110
Msa1054250 MtrunA17_Chr3g0125001 26.154 390 265 12 63 437 50 431 1.27e-25 108
Msa1054250 MtrunA17_Chr4g0010331 26.074 349 228 11 63 392 50 387 5.35e-25 106
Msa1054250 MtrunA17_Chr4g0053081 26.396 394 248 16 61 429 57 433 5.81e-25 106
Msa1054250 MtrunA17_Chr8g0374641 27.047 403 244 19 58 429 49 432 6.22e-25 106
Msa1054250 MtrunA17_Chr3g0095731 25.455 385 240 12 45 394 41 413 6.95e-25 106
Msa1054250 MtrunA17_Chr3g0097841 32.212 208 125 3 244 435 33 240 7.31e-25 102
Msa1054250 MtrunA17_Chr4g0010361 26.154 390 255 14 63 429 50 429 9.10e-25 105
Msa1054250 MtrunA17_Chr4g0010311 27.132 387 244 13 68 429 55 428 1.13e-24 105
Msa1054250 MtrunA17_Chr3g0095721 25.130 386 244 12 45 394 41 417 3.83e-24 104
Msa1054250 MtrunA17_Chr4g0004691 28.571 175 121 2 14 188 3 173 5.35e-24 98.6
Msa1054250 MtrunA17_Chr4g0053091 25.843 356 210 11 61 392 60 385 7.39e-24 103
Msa1054250 MtrunA17_Chr3g0134061 24.645 422 267 15 14 395 4 414 3.02e-23 101
Msa1054250 MtrunA17_Chr5g0408911 25.373 402 258 15 37 415 41 423 3.73e-23 101
Msa1054250 MtrunA17_Chr7g0273741 35.556 135 84 2 20 151 14 148 4.06e-23 95.1
Msa1054250 MtrunA17_Chr4g0010121 25.879 398 254 14 58 429 89 471 4.45e-23 101
Msa1054250 MtrunA17_Chr1g0199561 24.598 435 276 15 13 413 1 417 5.53e-23 100
Msa1054250 MtrunA17_Chr6g0471371 38.065 155 83 4 19 169 12 157 6.83e-23 95.1
Msa1054250 MtrunA17_Chr8g0354371 26.686 341 231 10 63 392 59 391 7.01e-23 100
Msa1054250 MtrunA17_Chr3g0098961 37.705 122 73 1 20 138 17 138 4.01e-22 93.2
Msa1054250 MtrunA17_Chr6g0467861 26.834 477 283 23 13 437 45 507 5.06e-22 98.6
Msa1054250 MtrunA17_Chr7g0274021 25.549 364 242 11 12 362 5 352 8.13e-22 97.4
Msa1054250 MtrunA17_Chr8g0376901 25.366 410 248 16 13 392 1 382 1.15e-21 96.7
Msa1054250 MtrunA17_Chr7g0224131 24.740 384 264 12 63 429 50 425 1.38e-21 96.7
Msa1054250 MtrunA17_Chr4g0010351 29.603 277 169 11 68 327 55 322 2.03e-21 95.5
Msa1054250 MtrunA17_Chr8g0361781 23.980 392 262 13 20 392 12 386 2.13e-21 96.3
Msa1054250 MtrunA17_Chr5g0436621 23.575 386 227 11 67 393 60 436 3.51e-21 95.9
Msa1054250 MtrunA17_Chr3g0113181 24.523 367 222 11 68 392 66 419 6.01e-21 95.1
Msa1054250 MtrunA17_Chr4g0010381 25.707 389 246 16 68 429 55 427 5.61e-20 91.7
Msa1054250 MtrunA17_Chr8g0361771 24.570 407 255 13 15 392 3 386 9.42e-20 91.3
Msa1054250 MtrunA17_Chr5g0438701 22.222 441 296 15 13 432 1 415 1.44e-19 90.5
Msa1054250 MtrunA17_Chr3g0134101 27.419 310 193 9 56 356 74 360 1.78e-19 90.5
Msa1054250 MtrunA17_Chr1g0165741 34.815 135 85 2 20 151 13 147 2.60e-19 84.3
Msa1054250 MtrunA17_Chr3g0134111 25.063 399 247 16 25 390 19 398 6.04e-19 89.0
Msa1054250 MtrunA17_Chr7g0219331 24.148 352 235 10 68 391 157 504 2.27e-18 87.4
Msa1054250 MtrunA17_Chr2g0323481 23.333 360 236 11 68 392 62 416 4.21e-18 86.3
Msa1054250 MtrunA17_Chr4g0004701 26.702 191 119 5 265 435 49 238 1.09e-17 82.4
Msa1054250 MtrunA17_Chr2g0323501 25.208 361 231 13 68 392 73 430 1.49e-17 84.7
Msa1054250 MtrunA17_Chr7g0219291 23.587 407 261 14 68 435 416 811 1.70e-17 85.1
Msa1054250 MtrunA17_Chr7g0219241 23.430 414 260 16 67 435 68 469 1.87e-17 84.3
Msa1054250 MtrunA17_Chr7g0219301 24.716 352 233 10 68 391 64 411 3.98e-17 84.0
Msa1054250 MtrunA17_Chr2g0323421 24.650 357 234 9 68 392 111 464 1.07e-16 82.4
Msa1054250 MtrunA17_Chr7g0252761 32.374 139 91 3 20 156 20 157 3.92e-16 75.9
Msa1054250 MtrunA17_Chr8g0378151 29.609 179 123 2 24 201 20 196 1.43e-15 78.6
Msa1054250 MtrunA17_Chr3g0097851 38.202 89 55 0 265 353 26 114 3.50e-15 71.6
Msa1054250 MtrunA17_Chr6g0455341 24.571 350 231 12 68 391 70 412 7.48e-15 76.3
Msa1054250 MtrunA17_Chr1g0165731 29.204 113 75 3 165 276 1 109 8.55e-15 70.5
Msa1054250 MtrunA17_Chr7g0219181 22.596 416 264 15 67 435 67 471 2.15e-14 75.1
Msa1054250 MtrunA17_Chr2g0323511 23.797 374 239 14 67 399 74 442 2.27e-14 75.1
Msa1054250 MtrunA17_Chr6g0454761 22.006 309 214 9 150 435 634 938 1.24e-13 73.2
Msa1054250 MtrunA17_Chr6g0454761 23.134 268 172 8 150 391 147 406 7.02e-13 70.9
Msa1054250 MtrunA17_Chr7g0273701 33.028 109 70 2 292 397 1 109 1.45e-13 68.6
Msa1054250 MtrunA17_Chr2g0311411 31.447 159 95 5 13 170 3 148 1.51e-13 68.2
Msa1054250 MtrunA17_Chr8g0353421 34.906 106 66 2 21 123 14 119 2.88e-13 66.6
Msa1054250 MtrunA17_Chr2g0323471 21.289 357 241 9 67 391 81 429 4.30e-13 70.9
Msa1054250 MtrunA17_Chr1g0148851 22.485 338 211 12 48 359 39 351 1.04e-12 69.7
Msa1054250 MtrunA17_Chr6g0471891 26.061 165 92 5 234 396 1 137 1.13e-12 67.4
Msa1054250 MtrunA17_Chr8g0339641 22.252 373 243 11 67 397 61 428 3.28e-12 68.2
Msa1054250 MtrunA17_Chr2g0302471 28.947 152 97 5 15 165 4 145 5.65e-12 63.5
Msa1054250 MtrunA17_Chr3g0132831 23.629 237 160 7 48 275 46 270 1.12e-11 66.2
Msa1054250 MtrunA17_Chr7g0219121 23.588 301 173 12 62 315 61 351 1.80e-11 65.9
Msa1054250 MtrunA17_Chr3g0097831 36.986 73 44 1 24 94 10 82 2.83e-11 59.3
Msa1054250 MtrunA17_Chr3g0106631 32.051 156 98 6 151 302 2 153 9.76e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1054250 AT5G41040.1 73.210 433 110 4 11 437 25 457 0.0 672
Msa1054250 AT5G41040.2 73.210 433 110 4 11 437 9 441 0.0 671
Msa1054250 AT5G63560.1 61.520 421 152 4 20 437 13 426 0.0 530
Msa1054250 AT3G48720.1 55.609 419 182 3 21 437 14 430 2.34e-177 503
Msa1054250 AT5G48930.1 34.174 436 268 7 12 435 2 430 1.02e-81 258
Msa1054250 AT1G03390.1 35.747 442 264 14 13 437 19 457 1.66e-77 248
Msa1054250 AT1G65450.1 35.797 433 254 12 20 436 22 446 3.17e-77 247
Msa1054250 AT1G27620.1 33.724 427 260 9 19 435 12 425 8.52e-74 238
Msa1054250 AT5G17540.1 32.637 383 247 7 20 395 13 391 2.10e-64 214
Msa1054250 AT3G03480.1 34.726 383 232 10 23 395 26 400 1.77e-62 209
Msa1054250 AT5G57840.1 32.570 393 234 8 20 390 7 390 3.36e-57 195
Msa1054250 AT2G19070.1 31.750 400 239 11 26 396 14 408 7.26e-57 194
Msa1054250 AT1G28680.1 32.028 434 271 12 20 435 13 440 8.76e-57 194
Msa1054250 AT2G40230.1 30.787 432 271 11 20 435 9 428 2.82e-54 187
Msa1054250 AT1G28680.2 32.277 347 223 8 20 360 13 353 5.42e-50 174
Msa1054250 AT4G31910.1 30.189 424 259 13 31 423 26 443 3.63e-48 171
Msa1054250 AT5G07080.1 30.227 440 269 17 19 433 17 443 2.64e-45 163
Msa1054250 AT1G65450.3 32.292 288 177 7 161 436 1 282 8.03e-45 157
Msa1054250 AT3G47170.1 29.018 448 283 13 19 433 15 460 5.77e-42 154
Msa1054250 AT3G62160.1 29.923 391 236 12 22 397 11 378 1.45e-41 152
Msa1054250 AT2G25150.1 30.769 442 265 14 23 435 20 449 2.86e-41 152
Msa1054250 AT2G23510.1 28.894 443 272 16 23 436 20 448 6.66e-38 143
Msa1054250 AT1G78990.1 27.543 403 243 14 48 414 46 435 8.23e-35 135
Msa1054250 AT1G24430.1 27.818 417 254 17 35 432 27 415 3.60e-31 124
Msa1054250 AT1G31490.1 28.133 391 247 15 37 410 29 402 3.58e-30 122
Msa1054250 AT1G32910.1 25.341 367 245 11 46 393 44 400 6.11e-30 121
Msa1054250 AT5G16410.1 27.083 384 242 14 37 400 61 426 5.42e-28 116
Msa1054250 AT5G07870.1 29.073 313 195 9 62 356 66 369 5.02e-27 113
Msa1054250 AT5G42830.1 29.191 346 217 10 29 356 22 357 7.30e-27 112
Msa1054250 AT2G39980.1 26.190 378 216 11 68 392 62 429 8.29e-26 109
Msa1054250 AT3G29670.1 24.365 394 270 12 48 419 42 429 5.03e-24 104
Msa1054250 AT5G07860.1 27.832 309 194 8 62 356 66 359 5.25e-24 104
Msa1054250 AT5G23940.1 27.696 408 255 14 51 429 43 439 6.58e-24 104
Msa1054250 AT3G50270.1 26.718 393 249 17 68 434 62 441 8.97e-24 103
Msa1054250 AT5G61160.1 25.352 355 234 12 68 399 64 410 5.67e-23 101
Msa1054250 AT3G29635.1 24.548 387 266 11 71 436 70 451 5.70e-23 101
Msa1054250 AT4G15390.1 23.853 436 285 16 23 432 15 429 5.95e-23 101
Msa1054250 AT4G15390.2 23.853 436 285 16 23 432 15 429 5.95e-23 101
Msa1054250 AT1G24420.2 24.658 365 243 10 79 432 76 419 2.96e-22 99.0
Msa1054250 AT1G24420.1 24.658 365 243 10 79 432 76 419 2.96e-22 99.0
Msa1054250 AT3G30280.1 25.455 385 243 18 62 424 56 418 4.40e-20 92.4
Msa1054250 AT5G47950.1 24.439 401 254 17 48 432 42 409 4.92e-20 92.4
Msa1054250 AT3G50280.1 22.938 388 268 14 68 434 59 436 1.27e-19 91.3
Msa1054250 AT5G38130.1 24.523 367 249 12 71 419 82 438 8.46e-19 89.0
Msa1054250 AT5G23970.1 23.472 409 259 12 37 421 24 402 2.77e-18 87.0
Msa1054250 AT3G29590.1 25.220 341 223 13 76 392 69 401 4.20e-18 86.7
Msa1054250 AT1G65450.2 45.455 110 55 4 20 125 22 130 1.06e-17 80.9
Msa1054250 AT4G15400.1 24.356 427 288 17 23 433 12 419 1.36e-17 85.1
Msa1054250 AT5G07850.1 25.656 343 215 14 20 343 27 348 5.03e-17 83.6
Msa1054250 AT5G67150.1 23.243 370 237 13 68 407 61 413 6.62e-17 83.2
Msa1054250 AT3G29680.1 22.739 387 275 8 68 435 62 443 1.39e-16 82.0
Msa1054250 AT5G67160.1 23.947 380 264 14 68 433 56 424 1.14e-15 79.0
Msa1054250 AT3G50300.1 23.797 374 251 13 68 419 59 420 1.62e-15 78.6
Msa1054250 AT5G39090.1 22.751 378 265 11 78 436 75 444 5.08e-14 74.3
Msa1054250 AT4G29250.1 22.844 429 283 17 34 433 33 442 1.71e-13 72.4
Msa1054250 AT3G50280.2 22.623 305 216 10 142 434 10 306 2.01e-13 71.2
Msa1054250 AT1G03495.1 27.431 288 169 15 68 322 66 346 2.90e-13 71.2
Msa1054250 AT3G50290.1 25.283 265 182 7 137 389 9 269 7.41e-12 66.2
Msa1054250 AT1G03940.1 24.265 408 258 17 68 436 66 461 9.05e-12 67.4
Msa1054250 AT5G39050.1 21.725 313 219 6 150 436 152 464 4.40e-11 65.1

Find 97 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTCCAAAAGGGTATTT+TGG 0.109330 7_2:-25715531 Msa1054250:CDS
GTCTGGACCGGTTTCCTTGC+CGG 0.230469 7_2:-25715265 Msa1054250:CDS
CTTCAATGTCAGCAAATTCT+TGG 0.238280 7_2:+25715802 None:intergenic
AGAAGCTTTGTTTCTTGTTC+TGG 0.240151 7_2:+25715588 None:intergenic
GAAAATTCTAATTATCAAAA+TGG 0.257291 7_2:-25717342 Msa1054250:CDS
CACCATTTCATCTTCATATA+AGG 0.260329 7_2:+25715761 None:intergenic
TGCTTCACACAAATATGATA+TGG 0.268225 7_2:+25717530 None:intergenic
CCAAGATTTAATGCAGATAT+AGG 0.271308 7_2:-25715151 Msa1054250:exon
GTTCATTACTACCCTCTTGC+TGG 0.277776 7_2:-25717117 Msa1054250:CDS
CCCTTTCATACCACCGATTT+CGG 0.292690 7_2:-25715306 Msa1054250:CDS
CACTGTATGTTTGATGGAAT+TGG 0.294988 7_2:-25715945 Msa1054250:CDS
AATTGTTCAATGGAAGAAAT+TGG 0.299341 7_2:-25717015 Msa1054250:CDS
CCTTTCTTTGTTTCTTCAGA+TGG 0.304433 7_2:+25717270 None:intergenic
TCTTGACCGAAGCATATTAA+AGG 0.311197 7_2:-25715856 Msa1054250:CDS
AACAAGAGCTAGGCCATCTT+TGG 0.319090 7_2:-25715364 Msa1054250:CDS
CAAGTGTTGGTTCACTTGTT+AGG 0.323483 7_2:+25717298 None:intergenic
AGTGCTGTTTATGAATTTAT+TGG 0.329556 7_2:-25715052 Msa1054250:three_prime_UTR
AGAAGCATTAATGTGCTTCT+AGG 0.333464 7_2:-25715201 Msa1054250:CDS
TGGTGCAAATGCTCTATCTT+CGG 0.344041 7_2:+25715822 None:intergenic
CCATTCCCAAAATACCCTTT+TGG 0.354945 7_2:+25715525 None:intergenic
TATTCTTCAGATGCCTCCTT+TGG 0.367285 7_2:-25716926 Msa1054250:CDS
ATATTTGTGTGAAGCAATTC+AGG 0.371213 7_2:-25717523 Msa1054250:intron
GGTGCAAATGCTCTATCTTC+GGG 0.375653 7_2:+25715823 None:intergenic
CGATTTCGGTTGGGGAGAAC+CGG 0.379270 7_2:-25715292 Msa1054250:CDS
GAATGCTTGTGAAGTGATTA+AGG 0.383599 7_2:-25717160 Msa1054250:CDS
CCTATATCTGCATTAAATCT+TGG 0.386363 7_2:+25715151 None:intergenic
TCATACCACCGATTTCGGTT+GGG 0.393719 7_2:-25715301 Msa1054250:CDS
AAGTGCTTTCAGCATTTGTT+TGG 0.399533 7_2:-25715641 Msa1054250:CDS
TCCTTCTCCGGCAAGGAAAC+CGG 0.400213 7_2:+25715258 None:intergenic
GATGCCTCCTTTGGTTGCTC+AGG 0.401248 7_2:-25716917 Msa1054250:intron
CGGTTCTCCCCAACCGAAAT+CGG 0.413358 7_2:+25715293 None:intergenic
TGGGGAGAACCGGTTCTGTC+TGG 0.419323 7_2:-25715282 Msa1054250:CDS
TTCATATAAGGTACTAGTGT+TGG 0.419653 7_2:+25715773 None:intergenic
TTCATACCACCGATTTCGGT+TGG 0.423100 7_2:-25715302 Msa1054250:CDS
ATGAATCACTGTATGTTTGA+TGG 0.425031 7_2:-25715951 Msa1054250:CDS
TACCTTATATGAAGATGAAA+TGG 0.425519 7_2:-25715763 Msa1054250:CDS
TGCGGACCTTTAATATGCTT+CGG 0.427426 7_2:+25715850 None:intergenic
AATATGCTTCGGTCAAGAAC+TGG 0.431411 7_2:+25715861 None:intergenic
AACAAAATCACTTCCTTCTC+CGG 0.435908 7_2:+25715246 None:intergenic
CATACCACCGATTTCGGTTG+GGG 0.437822 7_2:-25715300 Msa1054250:CDS
TCCAAGGACTCTTGGTATGT+TGG 0.438373 7_2:-25716974 Msa1054250:CDS
TTTGAACCAAAACTTCCAAA+AGG 0.445633 7_2:-25715540 Msa1054250:CDS
TTCTTTGTTTCTTCAGATGG+TGG 0.446811 7_2:+25717273 None:intergenic
AACCTTCACCGTATACGAAA+AGG 0.447710 7_2:+25715452 None:intergenic
ACCAATGCTGTTTGTAAAGC+AGG 0.450940 7_2:-25715492 Msa1054250:CDS
GCTTCACACAAATATGATAT+GGG 0.459751 7_2:+25717531 None:intergenic
AAACCTGATCCAAGGACTCT+TGG 0.464374 7_2:-25716982 Msa1054250:CDS
TTCATTACTACCCTCTTGCT+GGG 0.465589 7_2:-25717116 Msa1054250:CDS
AGCTGAAGCTAATTGTTCAA+TGG 0.473226 7_2:-25717025 Msa1054250:CDS
ATACCAAGAGTCCTTGGATC+AGG 0.478213 7_2:+25716979 None:intergenic
ACCAACATACCAAGAGTCCT+TGG 0.479611 7_2:+25716973 None:intergenic
TCAGATGGTGGAACAAGTGT+TGG 0.489688 7_2:+25717285 None:intergenic
ACCGGTTTCCTTGCCGGAGA+AGG 0.494266 7_2:-25715259 Msa1054250:CDS
CTATGGAGTTTGTTAACTCA+TGG 0.498485 7_2:-25715920 Msa1054250:CDS
ACCTGCTTTACAAACAGCAT+TGG 0.499694 7_2:+25715491 None:intergenic
ACCGAAATCGGTGGTATGAA+AGG 0.500259 7_2:+25715305 None:intergenic
TTGGTAGTAAGCAATAACTA+AGG 0.501457 7_2:-25715033 Msa1054250:three_prime_UTR
AAAACCTCCACATTTGAACT+TGG 0.504811 7_2:+25715989 None:intergenic
CTCAGAGGGTAAACTGATTG+TGG 0.510531 7_2:-25717079 Msa1054250:CDS
TGAAGAATATGCTTAGCATC+AGG 0.522838 7_2:+25716940 None:intergenic
ACCTTCACCGTATACGAAAA+GGG 0.526148 7_2:+25715453 None:intergenic
AAGATCTTCATGACAGGAGC+AGG 0.527288 7_2:+25715174 None:intergenic
TTGAACCAAAACTTCCAAAA+GGG 0.529877 7_2:-25715539 Msa1054250:CDS
CCGAAATCGGTGGTATGAAA+GGG 0.531154 7_2:+25715306 None:intergenic
TATGGAGTTTGTTAACTCAT+GGG 0.537855 7_2:-25715919 Msa1054250:CDS
TCCTCTCTTGACCATGTGAA+AGG 0.539507 7_2:+25715223 None:intergenic
GTTTGATGGAATTGGAGCTA+TGG 0.548532 7_2:-25715937 Msa1054250:CDS
TAATCAAGAAGTGTTTGAGA+TGG 0.549573 7_2:-25717364 Msa1054250:exon
GAAACCGGTCCAGACAGAAC+CGG 0.551440 7_2:+25715273 None:intergenic
AAAAGTGTACAAGCCAAAGA+TGG 0.555885 7_2:+25715351 None:intergenic
AGAACCGGTTCTGTCTGGAC+CGG 0.557075 7_2:-25715277 Msa1054250:CDS
TATTCAAGATGCAATCAAGA+TGG 0.561804 7_2:-25715430 Msa1054250:CDS
CCATCTGAAGAAACAAAGAA+AGG 0.565357 7_2:-25717270 Msa1054250:CDS
AAACTGATTGTGGATTGTAC+TGG 0.568236 7_2:-25717069 Msa1054250:CDS
AGAACTGGAGGGACGGAGAG+TGG 0.571956 7_2:+25715876 None:intergenic
TCTTGGAAGATCTTCATGAC+AGG 0.573252 7_2:+25715168 None:intergenic
TCCTTTCACATGGTCAAGAG+AGG 0.581268 7_2:-25715224 Msa1054250:CDS
AGATTAAGTATAAGCTCAGA+GGG 0.587672 7_2:-25717093 Msa1054250:CDS
CAGCTGACCAAGTTCAAATG+TGG 0.590914 7_2:-25715996 Msa1054250:CDS
ATGGAGTTTGTTAACTCATG+GGG 0.595449 7_2:-25715918 Msa1054250:CDS
GTGCAAATGCTCTATCTTCG+GGG 0.597277 7_2:+25715824 None:intergenic
AATCACTTCCTTCTCCGGCA+AGG 0.599797 7_2:+25715251 None:intergenic
CTGACCAAGTTCAAATGTGG+AGG 0.605830 7_2:-25715993 Msa1054250:CDS
ATACTTAATCTCCCAGCAAG+AGG 0.610225 7_2:+25717105 None:intergenic
TGCAAATGCTCTATCTTCGG+GGG 0.613923 7_2:+25715825 None:intergenic
ATGCTTCGGTCAAGAACTGG+AGG 0.620290 7_2:+25715864 None:intergenic
ATATTACAAAACCTGATCCA+AGG 0.621561 7_2:-25716990 Msa1054250:CDS
TCGGTCAAGAACTGGAGGGA+CGG 0.623713 7_2:+25715869 None:intergenic
CATACCTGAGCAACCAAAGG+AGG 0.626730 7_2:+25716913 None:intergenic
TACTTAATCTCCCAGCAAGA+GGG 0.629161 7_2:+25717106 None:intergenic
AAACATACCTGAGCAACCAA+AGG 0.647608 7_2:+25716910 None:intergenic
GAGATTAAGTATAAGCTCAG+AGG 0.659227 7_2:-25717094 Msa1054250:CDS
TCATGGGGAGAAATAGCAAG+AGG 0.661263 7_2:-25715903 Msa1054250:CDS
AAAGCTTTAGAAGATGAAGG+TGG 0.665451 7_2:-25715690 Msa1054250:CDS
TTCTCCCCAACCGAAATCGG+TGG 0.671335 7_2:+25715296 None:intergenic
ATTGTGGATTGTACTGGAGA+AGG 0.681300 7_2:-25717063 Msa1054250:CDS
TGCTTCGGTCAAGAACTGGA+GGG 0.700549 7_2:+25715865 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTAATTTTTTTAGTTA+AGG - chr7_2:25716010-25716029 Msa1054250:intron 10.0%
!!! TGTTTAATTTTTTTAGTTAA+GGG - chr7_2:25716011-25716030 Msa1054250:intron 10.0%
!!! TTGTGTTATTTTTTATTATA+TGG - chr7_2:25717480-25717499 Msa1054250:intron 10.0%
!! GAAAATTCTAATTATCAAAA+TGG - chr7_2:25715242-25715261 Msa1054250:CDS 15.0%
!! CCAAATTATTTAAAAGTTTA+GGG + chr7_2:25715901-25715920 None:intergenic 15.0%
!! TCCAAATTATTTAAAAGTTT+AGG + chr7_2:25715902-25715921 None:intergenic 15.0%
!!! CTTTTAAATAATTTGGAATT+TGG - chr7_2:25715905-25715924 Msa1054250:CDS 15.0%
!! AAAAATATTTAGTACATGTT+TGG + chr7_2:25716137-25716156 None:intergenic 15.0%
!!! CTATTTGAGAAATTTTAAAT+AGG + chr7_2:25716203-25716222 None:intergenic 15.0%
!! ATTTATGTATGATAAACATA+AGG - chr7_2:25716362-25716381 Msa1054250:intron 15.0%
!! AATGTTATGTTTGAAGATTA+AGG + chr7_2:25715156-25715175 None:intergenic 20.0%
!!! TTTTTGTTTGTTTGTAAAAC+AGG - chr7_2:25715192-25715211 Msa1054250:CDS 20.0%
!!! CCCTAAACTTTTAAATAATT+TGG - chr7_2:25715898-25715917 Msa1054250:CDS 20.0%
!!! TTTTAGAAATATAGTTGATG+CGG - chr7_2:25716520-25716539 Msa1054250:intron 20.0%
! TTGTTTCAAAAATCAAGAGA+AGG - chr7_2:25715394-25715413 Msa1054250:CDS 25.0%
! TGTTTCAAAAATCAAGAGAA+GGG - chr7_2:25715395-25715414 Msa1054250:CDS 25.0%
! AATTGTTCAATGGAAGAAAT+TGG - chr7_2:25715569-25715588 Msa1054250:CDS 25.0%
! ACATGATTCAATGTATTCAT+AGG - chr7_2:25715803-25715822 Msa1054250:CDS 25.0%
! TTAAGAGGTTACTTGTAATT+TGG - chr7_2:25715873-25715892 Msa1054250:CDS 25.0%
! TAAGAGGTTACTTGTAATTT+GGG - chr7_2:25715874-25715893 Msa1054250:CDS 25.0%
! ACTTCAGTGACAAAAAATAA+AGG + chr7_2:25716248-25716267 None:intergenic 25.0%
!! TACTTTTGAACTTAGACATT+TGG + chr7_2:25716277-25716296 None:intergenic 25.0%
! TACCTTATATGAAGATGAAA+TGG - chr7_2:25716821-25716840 Msa1054250:intron 25.0%
!!! TTCAATTCTTTTAGTTTCTC+GGG + chr7_2:25716870-25716889 None:intergenic 25.0%
!!! CTTCAATTCTTTTAGTTTCT+CGG + chr7_2:25716871-25716890 None:intergenic 25.0%
!!! AAAAAAGCTTTAGAAGATGA+AGG - chr7_2:25716891-25716910 Msa1054250:intron 25.0%
!!! ATCAGCTATTGATTATTTTG+AGG - chr7_2:25717196-25717215 Msa1054250:CDS 25.0%
!!! AGTGCTGTTTATGAATTTAT+TGG - chr7_2:25717532-25717551 Msa1054250:five_prime_UTR 25.0%
GCTTCACACAAATATGATAT+GGG + chr7_2:25715056-25715075 None:intergenic 30.0%
TGCTTCACACAAATATGATA+TGG + chr7_2:25715057-25715076 None:intergenic 30.0%
ATATTTGTGTGAAGCAATTC+AGG - chr7_2:25715061-25715080 Msa1054250:three_prime_UTR 30.0%
!! TAATCAAGAAGTGTTTGAGA+TGG - chr7_2:25715220-25715239 Msa1054250:CDS 30.0%
!!! TGTTTTGATCAAGGTTTGAT+AGG + chr7_2:25715349-25715368 None:intergenic 30.0%
!!! TTACAGCAATGTTTTGATCA+AGG + chr7_2:25715358-25715377 None:intergenic 30.0%
AGATTAAGTATAAGCTCAGA+GGG - chr7_2:25715491-25715510 Msa1054250:CDS 30.0%
ATATTACAAAACCTGATCCA+AGG - chr7_2:25715594-25715613 Msa1054250:CDS 30.0%
!!! GTTACTAGTCTTTTTGTAGT+TGG - chr7_2:25715698-25715717 Msa1054250:CDS 30.0%
TCTCAAATTATTATCCGTGT+CGG + chr7_2:25715778-25715797 None:intergenic 30.0%
ATGAATCACTGTATGTTTGA+TGG - chr7_2:25716633-25716652 Msa1054250:intron 30.0%
!! TATGGAGTTTGTTAACTCAT+GGG - chr7_2:25716665-25716684 Msa1054250:intron 30.0%
! TTCATATAAGGTACTAGTGT+TGG + chr7_2:25716814-25716833 None:intergenic 30.0%
CACCATTTCATCTTCATATA+AGG + chr7_2:25716826-25716845 None:intergenic 30.0%
TTTGAACCAAAACTTCCAAA+AGG - chr7_2:25717044-25717063 Msa1054250:CDS 30.0%
TTGAACCAAAACTTCCAAAA+GGG - chr7_2:25717045-25717064 Msa1054250:CDS 30.0%
!! AAATACCCTTTTGGAAGTTT+TGG + chr7_2:25717053-25717072 None:intergenic 30.0%
!!! AAACTTCCAAAAGGGTATTT+TGG - chr7_2:25717053-25717072 Msa1054250:CDS 30.0%
!!! AACTTCCAAAAGGGTATTTT+GGG - chr7_2:25717054-25717073 Msa1054250:CDS 30.0%
TATTCAAGATGCAATCAAGA+TGG - chr7_2:25717154-25717173 Msa1054250:CDS 30.0%
CCTATATCTGCATTAAATCT+TGG + chr7_2:25717436-25717455 None:intergenic 30.0%
CCAAGATTTAATGCAGATAT+AGG - chr7_2:25717433-25717452 Msa1054250:intron 30.0%
! TTGGTAGTAAGCAATAACTA+AGG - chr7_2:25717551-25717570 Msa1054250:five_prime_UTR 30.0%
GCAATGTTGAAAATGTCACA+TGG + chr7_2:25715118-25715137 None:intergenic 35.0%
! TTCTTTGTTTCTTCAGATGG+TGG + chr7_2:25715314-25715333 None:intergenic 35.0%
! CCTTTCTTTGTTTCTTCAGA+TGG + chr7_2:25715317-25715336 None:intergenic 35.0%
CCATCTGAAGAAACAAAGAA+AGG - chr7_2:25715314-25715333 Msa1054250:CDS 35.0%
GAATGCTTGTGAAGTGATTA+AGG - chr7_2:25715424-25715443 Msa1054250:CDS 35.0%
GAGATTAAGTATAAGCTCAG+AGG - chr7_2:25715490-25715509 Msa1054250:CDS 35.0%
! AAACTGATTGTGGATTGTAC+TGG - chr7_2:25715515-25715534 Msa1054250:CDS 35.0%
AGCTGAAGCTAATTGTTCAA+TGG - chr7_2:25715559-25715578 Msa1054250:CDS 35.0%
TGAAGAATATGCTTAGCATC+AGG + chr7_2:25715647-25715666 None:intergenic 35.0%
!! TAGTTACTTGCTATGAACCA+CGG - chr7_2:25715735-25715754 Msa1054250:CDS 35.0%
TACAAGTAACCTCTTAAGCA+CGG + chr7_2:25715870-25715889 None:intergenic 35.0%
TCAAGTGTATCTTTGATGAG+CGG - chr7_2:25716040-25716059 Msa1054250:intron 35.0%
! TGTCTCACAAGTATCGTAAA+AGG - chr7_2:25716398-25716417 Msa1054250:intron 35.0%
! CGTCGCAATATTTTATATCG+TGG - chr7_2:25716492-25716511 Msa1054250:intron 35.0%
TCATGTTGCAACTGTAATTG+TGG + chr7_2:25716546-25716565 None:intergenic 35.0%
AAAACCTCCACATTTGAACT+TGG + chr7_2:25716598-25716617 None:intergenic 35.0%
! AAATGTGGAGGTTTTTCTCT+TGG - chr7_2:25716603-25716622 Msa1054250:intron 35.0%
CACTGTATGTTTGATGGAAT+TGG - chr7_2:25716639-25716658 Msa1054250:intron 35.0%
!! CTATGGAGTTTGTTAACTCA+TGG - chr7_2:25716664-25716683 Msa1054250:intron 35.0%
!! ATGGAGTTTGTTAACTCATG+GGG - chr7_2:25716666-25716685 Msa1054250:intron 35.0%
TCTTGACCGAAGCATATTAA+AGG - chr7_2:25716728-25716747 Msa1054250:intron 35.0%
CTTCAATGTCAGCAAATTCT+TGG + chr7_2:25716785-25716804 None:intergenic 35.0%
!! AAAGCTTTAGAAGATGAAGG+TGG - chr7_2:25716894-25716913 Msa1054250:intron 35.0%
AAGTGCTTTCAGCATTTGTT+TGG - chr7_2:25716943-25716962 Msa1054250:CDS 35.0%
!! AGAAGCTTTGTTTCTTGTTC+TGG + chr7_2:25716999-25717018 None:intergenic 35.0%
!!! AAGCTTCTTTTTGCTGTTGA+TGG - chr7_2:25717011-25717030 Msa1054250:CDS 35.0%
!!! TTCTTTTTGCTGTTGATGGA+AGG - chr7_2:25717015-25717034 Msa1054250:CDS 35.0%
!!! TCTTTTTGCTGTTGATGGAA+GGG - chr7_2:25717016-25717035 Msa1054250:CDS 35.0%
!!! ATTTTGAGGTAACAAGAGCT+AGG - chr7_2:25717210-25717229 Msa1054250:CDS 35.0%
! AAAAGTGTACAAGCCAAAGA+TGG + chr7_2:25717236-25717255 None:intergenic 35.0%
! GTACACTTTTGATCACAACT+TGG - chr7_2:25717246-25717265 Msa1054250:CDS 35.0%
! TTTTGATCACAACTTGGTCT+AGG - chr7_2:25717252-25717271 Msa1054250:CDS 35.0%
AACAAAATCACTTCCTTCTC+CGG + chr7_2:25717341-25717360 None:intergenic 35.0%
! GTGATTTTGTTCCTTTCACA+TGG - chr7_2:25717350-25717369 Msa1054250:exon 35.0%
!! AGAAGCATTAATGTGCTTCT+AGG - chr7_2:25717383-25717402 Msa1054250:intron 35.0%
!! CAAGTGTTGGTTCACTTGTT+AGG + chr7_2:25715289-25715308 None:intergenic 40.0%
TTCATTACTACCCTCTTGCT+GGG - chr7_2:25715468-25715487 Msa1054250:CDS 40.0%
TACTTAATCTCCCAGCAAGA+GGG + chr7_2:25715481-25715500 None:intergenic 40.0%
ATACTTAATCTCCCAGCAAG+AGG + chr7_2:25715482-25715501 None:intergenic 40.0%
! ATTGTGGATTGTACTGGAGA+AGG - chr7_2:25715521-25715540 Msa1054250:CDS 40.0%
TATTCTTCAGATGCCTCCTT+TGG - chr7_2:25715658-25715677 Msa1054250:CDS 40.0%
AAACATACCTGAGCAACCAA+AGG + chr7_2:25715677-25715696 None:intergenic 40.0%
! TGTATTCATAGGTGTCGTGT+CGG - chr7_2:25715814-25715833 Msa1054250:CDS 40.0%
AGCATTTCTCAAGCACTTGT+AGG - chr7_2:25716338-25716357 Msa1054250:intron 40.0%
GTTTGATGGAATTGGAGCTA+TGG - chr7_2:25716647-25716666 Msa1054250:intron 40.0%
AATATGCTTCGGTCAAGAAC+TGG + chr7_2:25716726-25716745 None:intergenic 40.0%
TGCGGACCTTTAATATGCTT+CGG + chr7_2:25716737-25716756 None:intergenic 40.0%
TGGTGCAAATGCTCTATCTT+CGG + chr7_2:25716765-25716784 None:intergenic 40.0%
! CCATTCCCAAAATACCCTTT+TGG + chr7_2:25717062-25717081 None:intergenic 40.0%
!!! CCAAAAGGGTATTTTGGGAA+TGG - chr7_2:25717059-25717078 Msa1054250:CDS 40.0%
! ACCAATGCTGTTTGTAAAGC+AGG - chr7_2:25717092-25717111 Msa1054250:CDS 40.0%
ACCTGCTTTACAAACAGCAT+TGG + chr7_2:25717096-25717115 None:intergenic 40.0%
! GAACAAGCCCTTTTCGTATA+CGG - chr7_2:25717124-25717143 Msa1054250:CDS 40.0%
ACCTTCACCGTATACGAAAA+GGG + chr7_2:25717134-25717153 None:intergenic 40.0%
AACCTTCACCGTATACGAAA+AGG + chr7_2:25717135-25717154 None:intergenic 40.0%
TCTTGGAAGATCTTCATGAC+AGG + chr7_2:25717419-25717438 None:intergenic 40.0%
!! TCAGATGGTGGAACAAGTGT+TGG + chr7_2:25715302-25715321 None:intergenic 45.0%
GTTCATTACTACCCTCTTGC+TGG - chr7_2:25715467-25715486 Msa1054250:CDS 45.0%
CTCAGAGGGTAAACTGATTG+TGG - chr7_2:25715505-25715524 Msa1054250:CDS 45.0%
AAACCTGATCCAAGGACTCT+TGG - chr7_2:25715602-25715621 Msa1054250:CDS 45.0%
ATACCAAGAGTCCTTGGATC+AGG + chr7_2:25715608-25715627 None:intergenic 45.0%
TCCAAGGACTCTTGGTATGT+TGG - chr7_2:25715610-25715629 Msa1054250:CDS 45.0%
ACCAACATACCAAGAGTCCT+TGG + chr7_2:25715614-25715633 None:intergenic 45.0%
! TCCTCATATTTGAGGTGTCC+CGG + chr7_2:25715843-25715862 None:intergenic 45.0%
CAGCTGACCAAGTTCAAATG+TGG - chr7_2:25716588-25716607 Msa1054250:intron 45.0%
CTGACCAAGTTCAAATGTGG+AGG - chr7_2:25716591-25716610 Msa1054250:intron 45.0%
TCATGGGGAGAAATAGCAAG+AGG - chr7_2:25716681-25716700 Msa1054250:intron 45.0%
TGCAAATGCTCTATCTTCGG+GGG + chr7_2:25716762-25716781 None:intergenic 45.0%
GTGCAAATGCTCTATCTTCG+GGG + chr7_2:25716763-25716782 None:intergenic 45.0%
GGTGCAAATGCTCTATCTTC+GGG + chr7_2:25716764-25716783 None:intergenic 45.0%
AACAAGAGCTAGGCCATCTT+TGG - chr7_2:25717220-25717239 Msa1054250:CDS 45.0%
CCGAAATCGGTGGTATGAAA+GGG + chr7_2:25717281-25717300 None:intergenic 45.0%
!! CCCTTTCATACCACCGATTT+CGG - chr7_2:25717278-25717297 Msa1054250:CDS 45.0%
ACCGAAATCGGTGGTATGAA+AGG + chr7_2:25717282-25717301 None:intergenic 45.0%
!! TTCATACCACCGATTTCGGT+TGG - chr7_2:25717282-25717301 Msa1054250:CDS 45.0%
!! TCATACCACCGATTTCGGTT+GGG - chr7_2:25717283-25717302 Msa1054250:CDS 45.0%
TCCTTTCACATGGTCAAGAG+AGG - chr7_2:25717360-25717379 Msa1054250:exon 45.0%
TCCTCTCTTGACCATGTGAA+AGG + chr7_2:25717364-25717383 None:intergenic 45.0%
AAGATCTTCATGACAGGAGC+AGG + chr7_2:25717413-25717432 None:intergenic 45.0%
CATACCTGAGCAACCAAAGG+AGG + chr7_2:25715674-25715693 None:intergenic 50.0%
TTGCTATGAACCACGGACAC+CGG - chr7_2:25715742-25715761 Msa1054250:CDS 50.0%
TCCGGGACACCTCAAATATG+AGG - chr7_2:25715839-25715858 Msa1054250:CDS 50.0%
ACGGACGCTCCTCATATTTG+AGG + chr7_2:25715851-25715870 None:intergenic 50.0%
TGCTTCGGTCAAGAACTGGA+GGG + chr7_2:25716722-25716741 None:intergenic 50.0%
ATGCTTCGGTCAAGAACTGG+AGG + chr7_2:25716723-25716742 None:intergenic 50.0%
! GCCCTTTTCGTATACGGTGA+AGG - chr7_2:25717130-25717149 Msa1054250:CDS 50.0%
!! CATACCACCGATTTCGGTTG+GGG - chr7_2:25717284-25717303 Msa1054250:CDS 50.0%
AATCACTTCCTTCTCCGGCA+AGG + chr7_2:25717336-25717355 None:intergenic 50.0%
GATGCCTCCTTTGGTTGCTC+AGG - chr7_2:25715667-25715686 Msa1054250:CDS 55.0%
! ATAGGTGTCGTGTCGGTGTC+CGG - chr7_2:25715821-25715840 Msa1054250:CDS 55.0%
TCGGTCAAGAACTGGAGGGA+CGG + chr7_2:25716718-25716737 None:intergenic 55.0%
GCTCTATCTTCGGGGGATTG+CGG + chr7_2:25716755-25716774 None:intergenic 55.0%
TTCTCCCCAACCGAAATCGG+TGG + chr7_2:25717291-25717310 None:intergenic 55.0%
CGGTTCTCCCCAACCGAAAT+CGG + chr7_2:25717294-25717313 None:intergenic 55.0%
CGATTTCGGTTGGGGAGAAC+CGG - chr7_2:25717292-25717311 Msa1054250:CDS 55.0%
! AGAACCGGTTCTGTCTGGAC+CGG - chr7_2:25717307-25717326 Msa1054250:CDS 55.0%
GAAACCGGTCCAGACAGAAC+CGG + chr7_2:25717314-25717333 None:intergenic 55.0%
TCCTTCTCCGGCAAGGAAAC+CGG + chr7_2:25717329-25717348 None:intergenic 55.0%
! TAGGTGTCGTGTCGGTGTCC+GGG - chr7_2:25715822-25715841 Msa1054250:CDS 60.0%
!! GAGGAGCGTCCGTGCTTAAG+AGG - chr7_2:25715858-25715877 Msa1054250:CDS 60.0%
AGAACTGGAGGGACGGAGAG+TGG + chr7_2:25716711-25716730 None:intergenic 60.0%
! TGGGGAGAACCGGTTCTGTC+TGG - chr7_2:25717302-25717321 Msa1054250:CDS 60.0%
! GTCTGGACCGGTTTCCTTGC+CGG - chr7_2:25717319-25717338 Msa1054250:CDS 60.0%
! ACCGGTTTCCTTGCCGGAGA+AGG - chr7_2:25717325-25717344 Msa1054250:CDS 60.0%
CCTGTCGTGTCCGGTGTCCG+TGG + chr7_2:25715755-25715774 None:intergenic 70.0%
CCACGGACACCGGACACGAC+AGG - chr7_2:25715752-25715771 Msa1054250:CDS 70.0%
! CCGTGTCGGCCTGTCGTGTC+CGG + chr7_2:25715764-25715783 None:intergenic 70.0%
CCGGACACGACAGGCCGACA+CGG - chr7_2:25715761-25715780 Msa1054250:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 25715022 25717584 25715022 ID=Msa1054250;Name=Msa1054250
chr7_2 mRNA 25715022 25717584 25715022 ID=Msa1054250-mRNA-1;Parent=Msa1054250;Name=Msa1054250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=86|1|1|1|0.5|0.33|3|130|437
chr7_2 exon 25715022 25716015 25715022 ID=Msa1054250-mRNA-1:exon:5528;Parent=Msa1054250-mRNA-1
chr7_2 exon 25716918 25717392 25716918 ID=Msa1054250-mRNA-1:exon:5527;Parent=Msa1054250-mRNA-1
chr7_2 exon 25717524 25717584 25717524 ID=Msa1054250-mRNA-1:exon:5526;Parent=Msa1054250-mRNA-1
chr7_2 five_prime_UTR 25717524 25717584 25717524 ID=Msa1054250-mRNA-1:five_prime_utr;Parent=Msa1054250-mRNA-1
chr7_2 five_prime_UTR 25717368 25717392 25717368 ID=Msa1054250-mRNA-1:five_prime_utr;Parent=Msa1054250-mRNA-1
chr7_2 CDS 25716918 25717367 25716918 ID=Msa1054250-mRNA-1:cds;Parent=Msa1054250-mRNA-1
chr7_2 CDS 25715152 25716015 25715152 ID=Msa1054250-mRNA-1:cds;Parent=Msa1054250-mRNA-1
chr7_2 three_prime_UTR 25715022 25715151 25715022 ID=Msa1054250-mRNA-1:three_prime_utr;Parent=Msa1054250-mRNA-1
Gene Sequence

>Msa1054250

ATGGAAAATTCTAATTATCAAAATGGCTTCAAACAAATTAATGTAAACCTAACAAGTGAACCAACACTTGTTCCACCATCTGAAGAAACAAAGAAAGGAATGTATTTCCTATCAAACCTTGATCAAAACATTGCTGTAATTGTTAGAACTGTTTATTGTTTCAAAAATCAAGAGAAGGGAAATGAGAATGCTTGTGAAGTGATTAAGGATGCATTGAAGAATGTTCTTGTTCATTACTACCCTCTTGCTGGGAGATTAAGTATAAGCTCAGAGGGTAAACTGATTGTGGATTGTACTGGAGAAGGTGCTCTTTTTGTTGAAGCTGAAGCTAATTGTTCAATGGAAGAAATTGGTGATATTACAAAACCTGATCCAAGGACTCTTGGTATGTTGGTTTATGATATTCCTGATGCTAAGCATATTCTTCAGATGCCTCCTTTGGTTGCTCAGCTGACCAAGTTCAAATGTGGAGGTTTTTCTCTTGGACTATGTATGAATCACTGTATGTTTGATGGAATTGGAGCTATGGAGTTTGTTAACTCATGGGGAGAAATAGCAAGAGGTTTACCACTCTCCGTCCCTCCAGTTCTTGACCGAAGCATATTAAAGGTCCGCAATCCCCCGAAGATAGAGCATTTGCACCAAGAATTTGCTGACATTGAAGACAAATCCAACACTAGTACCTTATATGAAGATGAAATGGTGTATCGATCATTCTGTTTCGATCCCGAGAAACTAAAAGAATTGAAGAAAAAAGCTTTAGAAGATGAAGGTGGTGTTCTTGAATCTTGCACAACATTTGAAGTGCTTTCAGCATTTGTTTGGATAGCTAGAACAAAAGCACTGAAAATGTTACCAGAACAAGAAACAAAGCTTCTTTTTGCTGTTGATGGAAGGGCTAAATTTGAACCAAAACTTCCAAAAGGGTATTTTGGGAATGGAATTATACTAACCAATGCTGTTTGTAAAGCAGGTGAAATTACGAACAAGCCCTTTTCGTATACGGTGAAGGTTATTCAAGATGCAATCAAGATGGTTACTGATAGTTACATGAGATCAGCTATTGATTATTTTGAGGTAACAAGAGCTAGGCCATCTTTGGCTTGTACACTTTTGATCACAACTTGGTCTAGGCTTCCCTTTCATACCACCGATTTCGGTTGGGGAGAACCGGTTCTGTCTGGACCGGTTTCCTTGCCGGAGAAGGAAGTGATTTTGTTCCTTTCACATGGTCAAGAGAGGAGAAGCATTAATGTGCTTCTAGGTTTACCTGCTCCTGTCATGAAGATCTTCCAAGATTTAATGCAGATATAG
Protein sequence

>Msa1054250

MENSNYQNGFKQINVNLTSEPTLVPPSEETKKGMYFLSNLDQNIAVIVRTVYCFKNQEKGNENACEVIKDALKNVLVHYYPLAGRLSISSEGKLIVDCTGEGALFVEAEANCSMEEIGDITKPDPRTLGMLVYDIPDAKHILQMPPLVAQLTKFKCGGFSLGLCMNHCMFDGIGAMEFVNSWGEIARGLPLSVPPVLDRSILKVRNPPKIEHLHQEFADIEDKSNTSTLYEDEMVYRSFCFDPEKLKELKKKALEDEGGVLESCTTFEVLSAFVWIARTKALKMLPEQETKLLFAVDGRAKFEPKLPKGYFGNGIILTNAVCKAGEITNKPFSYTVKVIQDAIKMVTDSYMRSAIDYFEVTRARPSLACTLLITTWSRLPFHTTDFGWGEPVLSGPVSLPEKEVILFLSHGQERRSINVLLGLPAPVMKIFQDLMQI*