Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0808160 | AES97503.1 | 92.517 | 147 | 11 | 0 | 1 | 147 | 86 | 232 | 1.53e-94 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0808160 | G7JZF8 | 92.517 | 147 | 11 | 0 | 1 | 147 | 86 | 232 | 7.30e-95 | 283 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0808160 | Msa0808170 | 0.952395 | 2.655418e-110 | -8.615850e-47 |
| Msa0808160 | Msa0817860 | 0.803876 | 2.827333e-49 | -8.615850e-47 |
| Msa0808160 | Msa1050500 | 0.804768 | 1.843792e-49 | -8.615850e-47 |
| Msa0808160 | Msa1135660 | 0.800479 | 1.413682e-48 | -8.615850e-47 |
| Msa0808160 | Msa1238130 | 0.814078 | 1.850005e-51 | -8.615850e-47 |
| Msa0808160 | Msa1279860 | 0.802377 | 5.775761e-49 | -8.615850e-47 |
| Msa0808160 | Msa1287700 | 0.821929 | 3.109203e-53 | -8.615850e-47 |
| Msa0481320 | Msa0808160 | 0.807257 | 5.520821e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0808160 | MtrunA17_Chr5g0423041 | 73.514 | 185 | 11 | 1 | 1 | 147 | 86 | 270 | 1.01e-90 | 264 |
| Msa0808160 | MtrunA17_Chr5g0423061 | 47.059 | 170 | 58 | 8 | 1 | 144 | 83 | 246 | 3.20e-32 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGAATTGGCTCTAATTC+AGG | 0.216122 | 5_3:-31979994 | None:intergenic |
| GCTCTAATTCAGGGAAATTT+AGG | 0.217699 | 5_3:-31979984 | None:intergenic |
| AAATGACGATCATTTCTCTT+AGG | 0.261854 | 5_3:-31980464 | None:intergenic |
| AACTGAAATTTAAAGTTTCC+TGG | 0.308434 | 5_3:-31980299 | None:intergenic |
| AATAATTCAGTTATCATATA+TGG | 0.311793 | 5_3:+31979961 | Msa0808160:CDS |
| TTGAAAGTTTCAAATGGAAC+TGG | 0.324695 | 5_3:-31980186 | None:intergenic |
| TGTGGAGGATGTTATCTTGC+AGG | 0.342062 | 5_3:+31980258 | Msa0808160:CDS |
| GGGAACATGGGAGGTTTATC+AGG | 0.359493 | 5_3:-31980141 | None:intergenic |
| CATTTGAAGGATGCCGTAAT+AGG | 0.369051 | 5_3:+31980482 | Msa0808160:CDS |
| TATATTTGAACTTGTTATCA+TGG | 0.377054 | 5_3:-31980080 | None:intergenic |
| AAGTTTGGCGTATTCTGAAT+TGG | 0.406948 | 5_3:-31980006 | None:intergenic |
| ATAGTGTTAATGCTGAAGTT+TGG | 0.408935 | 5_3:-31980021 | None:intergenic |
| AACTTCTATGTATGCAATCA+TGG | 0.414576 | 5_3:+31980045 | Msa0808160:CDS |
| TTAAATTTCAGTTGTGGCCC+AGG | 0.434450 | 5_3:+31980309 | Msa0808160:CDS |
| CGATCATTTCTCTTAGGAAT+CGG | 0.452060 | 5_3:-31980458 | None:intergenic |
| TCTGAATTGGCTCTAATTCA+GGG | 0.452523 | 5_3:-31979993 | None:intergenic |
| ACACAATTGAAAGTTTCAAA+TGG | 0.468211 | 5_3:-31980192 | None:intergenic |
| TTACAATAGAGTGCCTATTA+CGG | 0.496542 | 5_3:-31980495 | None:intergenic |
| GAGAAATGATCGTCATTTGA+AGG | 0.502367 | 5_3:+31980469 | Msa0808160:CDS |
| AAAGTTGATTTAGAGTCTTG+TGG | 0.561215 | 5_3:+31980240 | Msa0808160:CDS |
| GAAACTTTAAATTTCAGTTG+TGG | 0.573815 | 5_3:+31980303 | Msa0808160:CDS |
| TGAAAGTTTCAAATGGAACT+GGG | 0.581443 | 5_3:-31980185 | None:intergenic |
| AAAGTCATGTCTACTAAACC+AGG | 0.587810 | 5_3:+31980281 | Msa0808160:CDS |
| TCATTTCTCTTAGGAATCGG+AGG | 0.602178 | 5_3:-31980455 | None:intergenic |
| GGAACTGGGAAACATGCCAA+TGG | 0.619846 | 5_3:-31980171 | None:intergenic |
| GTTGATTTAGAGTCTTGTGG+AGG | 0.623581 | 5_3:+31980243 | Msa0808160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTCAGTTATCATATA+TGG | + | chr5_3:31979961-31979980 | Msa0808160:CDS | 15.0% |
| !!! | TATATTTGAACTTGTTATCA+TGG | - | chr5_3:31980083-31980102 | None:intergenic | 20.0% |
| ! | ACACAATTGAAAGTTTCAAA+TGG | - | chr5_3:31980195-31980214 | None:intergenic | 25.0% |
| !! | AACTGAAATTTAAAGTTTCC+TGG | - | chr5_3:31980302-31980321 | None:intergenic | 25.0% |
| ! | GAAACTTTAAATTTCAGTTG+TGG | + | chr5_3:31980303-31980322 | Msa0808160:CDS | 25.0% |
| ! | TTAGACTAATGTATTTACCT+GGG | - | chr5_3:31980329-31980348 | None:intergenic | 25.0% |
| ! | ATTAGACTAATGTATTTACC+TGG | - | chr5_3:31980330-31980349 | None:intergenic | 25.0% |
| ATAGTGTTAATGCTGAAGTT+TGG | - | chr5_3:31980024-31980043 | None:intergenic | 30.0% | |
| AACTTCTATGTATGCAATCA+TGG | + | chr5_3:31980045-31980064 | Msa0808160:CDS | 30.0% | |
| AATGGAAAAATAGGGAACAT+GGG | - | chr5_3:31980156-31980175 | None:intergenic | 30.0% | |
| AACATGCCAATGGAAAAATA+GGG | - | chr5_3:31980164-31980183 | None:intergenic | 30.0% | |
| AAACATGCCAATGGAAAAAT+AGG | - | chr5_3:31980165-31980184 | None:intergenic | 30.0% | |
| TGAAAGTTTCAAATGGAACT+GGG | - | chr5_3:31980188-31980207 | None:intergenic | 30.0% | |
| TTGAAAGTTTCAAATGGAAC+TGG | - | chr5_3:31980189-31980208 | None:intergenic | 30.0% | |
| ! | AAAGTTGATTTAGAGTCTTG+TGG | + | chr5_3:31980240-31980259 | Msa0808160:CDS | 30.0% |
| AAATGACGATCATTTCTCTT+AGG | - | chr5_3:31980467-31980486 | None:intergenic | 30.0% | |
| ! | TTACAATAGAGTGCCTATTA+CGG | - | chr5_3:31980498-31980517 | None:intergenic | 30.0% |
| GCTCTAATTCAGGGAAATTT+AGG | - | chr5_3:31979987-31980006 | None:intergenic | 35.0% | |
| !! | TCTGAATTGGCTCTAATTCA+GGG | - | chr5_3:31979996-31980015 | None:intergenic | 35.0% |
| !! | TTCTGAATTGGCTCTAATTC+AGG | - | chr5_3:31979997-31980016 | None:intergenic | 35.0% |
| AAGTTTGGCGTATTCTGAAT+TGG | - | chr5_3:31980009-31980028 | None:intergenic | 35.0% | |
| CAATGGAAAAATAGGGAACA+TGG | - | chr5_3:31980157-31980176 | None:intergenic | 35.0% | |
| !! | CATGTTCCCTATTTTTCCAT+TGG | + | chr5_3:31980155-31980174 | Msa0808160:CDS | 35.0% |
| AAAGTCATGTCTACTAAACC+AGG | + | chr5_3:31980281-31980300 | Msa0808160:CDS | 35.0% | |
| ATGCTCTAACACTCATTCTT+TGG | + | chr5_3:31980348-31980367 | Msa0808160:intron | 35.0% | |
| CGATCATTTCTCTTAGGAAT+CGG | - | chr5_3:31980461-31980480 | None:intergenic | 35.0% | |
| GAGAAATGATCGTCATTTGA+AGG | + | chr5_3:31980469-31980488 | Msa0808160:CDS | 35.0% | |
| ! | GTTGATTTAGAGTCTTGTGG+AGG | + | chr5_3:31980243-31980262 | Msa0808160:CDS | 40.0% |
| TTAAATTTCAGTTGTGGCCC+AGG | + | chr5_3:31980309-31980328 | Msa0808160:CDS | 40.0% | |
| TCATTTCTCTTAGGAATCGG+AGG | - | chr5_3:31980458-31980477 | None:intergenic | 40.0% | |
| CATTTGAAGGATGCCGTAAT+AGG | + | chr5_3:31980482-31980501 | Msa0808160:CDS | 40.0% | |
| GGAAAAATAGGGAACATGGG+AGG | - | chr5_3:31980153-31980172 | None:intergenic | 45.0% | |
| TGTGGAGGATGTTATCTTGC+AGG | + | chr5_3:31980258-31980277 | Msa0808160:CDS | 45.0% | |
| GGGAACATGGGAGGTTTATC+AGG | - | chr5_3:31980144-31980163 | None:intergenic | 50.0% | |
| GGAACTGGGAAACATGCCAA+TGG | - | chr5_3:31980174-31980193 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 31979958 | 31980517 | 31979958 | ID=Msa0808160;Name=Msa0808160 |
| chr5_3 | mRNA | 31979958 | 31980517 | 31979958 | ID=Msa0808160-mRNA-1;Parent=Msa0808160;Name=Msa0808160-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|147 |
| chr5_3 | exon | 31979958 | 31980330 | 31979958 | ID=Msa0808160-mRNA-1:exon:10549;Parent=Msa0808160-mRNA-1 |
| chr5_3 | exon | 31980447 | 31980517 | 31980447 | ID=Msa0808160-mRNA-1:exon:10550;Parent=Msa0808160-mRNA-1 |
| chr5_3 | CDS | 31979958 | 31980330 | 31979958 | ID=Msa0808160-mRNA-1:cds;Parent=Msa0808160-mRNA-1 |
| chr5_3 | CDS | 31980447 | 31980517 | 31980447 | ID=Msa0808160-mRNA-1:cds;Parent=Msa0808160-mRNA-1 |
| Gene Sequence |
| Protein sequence |
