AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1050500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050500 MtrunA17_Chr7g0219651 87.879 99 12 0 3 101 1770 1868 4.67e-55 183
Msa1050500 MtrunA17_Chr7g0219651 80.198 101 14 1 3 97 774 874 9.91e-49 165
Msa1050500 MtrunA17_Chr7g0219571 86.869 99 13 0 3 101 785 883 1.71e-54 181
Msa1050500 MtrunA17_Chr7g0219581 66.667 108 27 1 3 101 209 316 1.91e-44 145
Msa1050500 MtrunA17_Chr4g0025961 68.519 108 25 1 3 101 778 885 6.02e-43 148
Msa1050500 MtrunA17_Chr7g0219601 66.667 108 27 1 3 101 769 876 1.25e-39 139
Msa1050500 MtrunA17_Chr7g0219611 65.657 99 29 1 3 101 765 858 2.15e-34 124
Msa1050500 MtrunA17_Chr7g0219621 95.349 43 2 0 3 45 176 218 3.00e-22 86.3
Msa1050500 MtrunA17_Chr7g0219671 49.541 109 32 5 3 90 783 889 2.80e-21 87.0
Msa1050500 MtrunA17_Chr7g0219721 45.283 106 40 3 3 90 783 888 2.30e-18 78.6
Msa1050500 MtrunA17_Chr7g0219781 48.624 109 33 5 3 90 786 892 4.68e-18 77.8
Msa1050500 MtrunA17_Chr7g0219691 44.340 106 41 3 3 90 783 888 3.79e-17 75.1
Msa1050500 MtrunA17_Chr7g0219761 62.791 43 16 0 3 45 38 80 4.36e-12 57.0
Msa1050500 MtrunA17_Chr7g0219681 65.116 43 15 0 3 45 774 816 2.91e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAACCTTTCTTTGAAAT+TGG 0.101855 7_2:-15252608 Msa1050500:CDS
ACATATAAGTGTTGAAGTTT+TGG 0.270609 7_2:+15252558 None:intergenic
AACAACCTTTCTTTGAAATT+GGG 0.351358 7_2:-15252607 Msa1050500:CDS
AAAGGTTGTTCTTGAGTACT+TGG 0.402756 7_2:+15252620 None:intergenic
AGTGAGGAAGATAGTCAGTT+TGG 0.417130 7_2:-15252740 Msa1050500:CDS
TTTAGAGTTTGCGTTGCAAT+TGG 0.422622 7_2:-15252768 Msa1050500:CDS
CTTCAATGAGTCAAGTGGTT+GGG 0.422708 7_2:-15252793 Msa1050500:CDS
TTCAATGAGTCAAGTGGTTG+GGG 0.429791 7_2:-15252792 Msa1050500:CDS
TGATTCCCAATTTCAAAGAA+AGG 0.432400 7_2:+15252602 None:intergenic
CGAGACGGTACTCTCCTTGT+AGG 0.441050 7_2:+15252649 None:intergenic
TTAAGTTGTTTGCATGATGA+TGG 0.466658 7_2:-15252827 Msa1050500:CDS
CCAACCACTTGACTCATTGA+AGG 0.486871 7_2:+15252794 None:intergenic
AGAGTTTGCGTTGCAATTGG+TGG 0.523981 7_2:-15252765 Msa1050500:CDS
CACGAGATCGCACACCTACA+AGG 0.527211 7_2:-15252663 Msa1050500:CDS
TAAGTTGTTTGCATGATGAT+GGG 0.556475 7_2:-15252826 Msa1050500:CDS
AGTACTTGGCCTCGCCGAGA+CGG 0.577496 7_2:+15252634 None:intergenic
CCTTCAATGAGTCAAGTGGT+TGG 0.599636 7_2:-15252794 Msa1050500:CDS
ACGACCTTCAATGAGTCAAG+TGG 0.623156 7_2:-15252798 Msa1050500:CDS
GTTGCAATTGGTGGTGAGTG+AGG 0.655883 7_2:-15252756 Msa1050500:CDS
CTACAAGGAGAGTACCGTCT+CGG 0.656380 7_2:-15252648 Msa1050500:CDS
TCAATGAGTCAAGTGGTTGG+GGG 0.661801 7_2:-15252791 Msa1050500:CDS
AAATTGGGAATCAAAGAACA+CGG 0.681680 7_2:-15252592 Msa1050500:CDS
AGGAGAGTACCGTCTCGGCG+AGG 0.710756 7_2:-15252643 Msa1050500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGGAATATTATTGCAAGT+TGG - chr7_2:15252563-15252582 Msa1050500:CDS 25.0%
!!! AACAACCTTTCTTTGAAATT+GGG - chr7_2:15252808-15252827 Msa1050500:CDS 25.0%
!!! ACATATAAGTGTTGAAGTTT+TGG + chr7_2:15252860-15252879 None:intergenic 25.0%
! TTAAGTTGTTTGCATGATGA+TGG - chr7_2:15252588-15252607 Msa1050500:CDS 30.0%
TAAGTTGTTTGCATGATGAT+GGG - chr7_2:15252589-15252608 Msa1050500:CDS 30.0%
!! GAACAACCTTTCTTTGAAAT+TGG - chr7_2:15252807-15252826 Msa1050500:CDS 30.0%
TGATTCCCAATTTCAAAGAA+AGG + chr7_2:15252816-15252835 None:intergenic 30.0%
AAATTGGGAATCAAAGAACA+CGG - chr7_2:15252823-15252842 Msa1050500:CDS 30.0%
! TTTAGAGTTTGCGTTGCAAT+TGG - chr7_2:15252647-15252666 Msa1050500:CDS 35.0%
! CATATATGCCCTCTCTTTTT+GGG + chr7_2:15252706-15252725 None:intergenic 35.0%
! TCATATATGCCCTCTCTTTT+TGG + chr7_2:15252707-15252726 None:intergenic 35.0%
AAAGGTTGTTCTTGAGTACT+TGG + chr7_2:15252798-15252817 None:intergenic 35.0%
CTTCAATGAGTCAAGTGGTT+GGG - chr7_2:15252622-15252641 Msa1050500:CDS 40.0%
TTCAATGAGTCAAGTGGTTG+GGG - chr7_2:15252623-15252642 Msa1050500:CDS 40.0%
AGTGAGGAAGATAGTCAGTT+TGG - chr7_2:15252675-15252694 Msa1050500:CDS 40.0%
ACGACCTTCAATGAGTCAAG+TGG - chr7_2:15252617-15252636 Msa1050500:CDS 45.0%
CCAACCACTTGACTCATTGA+AGG + chr7_2:15252624-15252643 None:intergenic 45.0%
CCTTCAATGAGTCAAGTGGT+TGG - chr7_2:15252621-15252640 Msa1050500:CDS 45.0%
TCAATGAGTCAAGTGGTTGG+GGG - chr7_2:15252624-15252643 Msa1050500:CDS 45.0%
AGAGTTTGCGTTGCAATTGG+TGG - chr7_2:15252650-15252669 Msa1050500:CDS 45.0%
TTGGTACGACCCAAAAAGAG+AGG - chr7_2:15252694-15252713 Msa1050500:CDS 45.0%
TGGTACGACCCAAAAAGAGA+GGG - chr7_2:15252695-15252714 Msa1050500:CDS 45.0%
!! GTTGCAATTGGTGGTGAGTG+AGG - chr7_2:15252659-15252678 Msa1050500:CDS 50.0%
!! CTACAAGGAGAGTACCGTCT+CGG - chr7_2:15252767-15252786 Msa1050500:CDS 50.0%
CACGAGATCGCACACCTACA+AGG - chr7_2:15252752-15252771 Msa1050500:CDS 55.0%
CGAGACGGTACTCTCCTTGT+AGG + chr7_2:15252769-15252788 None:intergenic 55.0%
AGTACTTGGCCTCGCCGAGA+CGG + chr7_2:15252784-15252803 None:intergenic 60.0%
!! AGGAGAGTACCGTCTCGGCG+AGG - chr7_2:15252772-15252791 Msa1050500:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 15252556 15252881 15252556 ID=Msa1050500;Name=Msa1050500
chr7_2 mRNA 15252556 15252881 15252556 ID=Msa1050500-mRNA-1;Parent=Msa1050500;Name=Msa1050500-mRNA-1;_AED=0.73;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|101
chr7_2 exon 15252876 15252881 15252876 ID=Msa1050500-mRNA-1:exon:3646;Parent=Msa1050500-mRNA-1
chr7_2 exon 15252556 15252855 15252556 ID=Msa1050500-mRNA-1:exon:3645;Parent=Msa1050500-mRNA-1
chr7_2 CDS 15252876 15252881 15252876 ID=Msa1050500-mRNA-1:cds;Parent=Msa1050500-mRNA-1
chr7_2 CDS 15252556 15252855 15252556 ID=Msa1050500-mRNA-1:cds;Parent=Msa1050500-mRNA-1
Gene Sequence

>Msa1050500

ATGAGTTTGGCGTTAAGTTGTTTGCATGATGATGGGACTCAACGACCTTCAATGAGTCAAGTGGTTGGGGGTTTAGAGTTTGCGTTGCAATTGGTGGTGAGTGAGGAAGATAGTCAGTTTGGTACGACCCAAAAAGAGAGGGCATATATGAGTGATGAATCGAGTGATTTGCATTTCACGAGATCGCACACCTACAAGGAGAGTACCGTCTCGGCGAGGCCAAGTACTCAAGAACAACCTTTCTTTGAAATTGGGAATCAAAGAACACGGTCGTATTCATGCCAAAACTTCAACACTTATATGTAG

Protein sequence

>Msa1050500

MSLALSCLHDDGTQRPSMSQVVGGLEFALQLVVSEEDSQFGTTQKERAYMSDESSDLHFTRSHTYKESTVSARPSTQEQPFFEIGNQRTRSYSCQNFNTYM*