AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0812370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCTCCTCTTTGTTAAAC+TGG 0.287597 5_3:+42340863 None:intergenic
ATCCAAGCACGCAAGGTTCT+AGG 0.333945 5_3:-42343331 Msa0812370:five_prime_UTR
ACCCTAGAACCTTGCGTGCT+TGG 0.362335 5_3:+42343329 None:intergenic
TCCCTCTCAACCGTGTTTGA+AGG 0.365061 5_3:-42343459 Msa0812370:five_prime_UTR
AAAATACCCTTGCCACATAA+GGG 0.394583 5_3:+42343543 None:intergenic
AAGAAAAGATGAAAAGTGTA+TGG 0.395163 5_3:-42340828 Msa0812370:CDS
ACTCAATACGCTTACATTTG+TGG 0.396401 5_3:+42343066 None:intergenic
GTACTGTACCTGATACAATA+TGG 0.402588 5_3:+42340996 None:intergenic
GTGAAAGATAAGAAAGATCT+TGG 0.408538 5_3:-42340913 Msa0812370:CDS
TCATTGGGGAAACATGAAAC+AGG 0.411716 5_3:-42343114 Msa0812370:five_prime_UTR
GAGGAGAACCATATTGTATC+AGG 0.416982 5_3:-42341004 Msa0812370:intron
CATATCAACATAAACATTCA+TGG 0.435825 5_3:+42341193 None:intergenic
GAAGTGAGTACTAATGTGTA+TGG 0.459975 5_3:-42343611 Msa0812370:five_prime_UTR
CTTGCCACATAAGGGGTGTA+TGG 0.467182 5_3:+42343551 None:intergenic
CGGTTGAGAGGGAGAAGAAA+GGG 0.467718 5_3:+42343469 None:intergenic
TGTGATGGGATCAGATCATT+GGG 0.482268 5_3:-42343129 Msa0812370:five_prime_UTR
TCAGAGCCACAATCGATTCA+AGG 0.485541 5_3:-42343418 Msa0812370:five_prime_UTR
GTTTAACAAAGAGGAGGGAT+TGG 0.488431 5_3:-42340860 Msa0812370:CDS
AACACTGGAAAGTTGGCTAA+TGG 0.488900 5_3:-42341275 Msa0812370:five_prime_UTR
CAATATGGTTCTCCTCTTCA+CGG 0.490112 5_3:+42341011 None:intergenic
GAGATCTAAGAGAGAGAAGA+GGG 0.490981 5_3:+42343491 None:intergenic
TTCAGAAGATTATGCTGTGA+TGG 0.493099 5_3:-42343144 Msa0812370:five_prime_UTR
ACGGTTGAGAGGGAGAAGAA+AGG 0.500542 5_3:+42343468 None:intergenic
GGGGCTGTAATCCATGGAAA+TGG 0.501769 5_3:-42341221 Msa0812370:five_prime_UTR
ACAGGACAACACTGGAAAGT+TGG 0.504393 5_3:-42341282 Msa0812370:intron
CTGTGATGGGATCAGATCAT+TGG 0.504690 5_3:-42343130 Msa0812370:five_prime_UTR
AAGATAAAAGATGAAAATCA+TGG 0.509621 5_3:+42343365 None:intergenic
TCCAAGCACGCAAGGTTCTA+GGG 0.512771 5_3:-42343330 Msa0812370:five_prime_UTR
TGAATCGATTGTGGCTCTGA+GGG 0.521754 5_3:+42343421 None:intergenic
TCATGGGGAGACCATTTCCA+TGG 0.527491 5_3:+42341210 None:intergenic
GAATATCTTGAGGAATATGT+TGG 0.532265 5_3:-42341036 Msa0812370:CDS
ACCTTCAAACACGGTTGAGA+GGG 0.532277 5_3:+42343458 None:intergenic
AGAAAAGATGAAAAGTGTAT+GGG 0.540681 5_3:-42340827 Msa0812370:CDS
GGAGATCTAAGAGAGAGAAG+AGG 0.543617 5_3:+42343490 None:intergenic
GTTGGAGGGGCTGTAATCCA+TGG 0.544476 5_3:-42341227 Msa0812370:five_prime_UTR
ATATCAACATAAACATTCAT+GGG 0.545871 5_3:+42341194 None:intergenic
TGGAAGAGTGGTATGGAGAA+GGG 0.549448 5_3:+42343571 None:intergenic
TCTTCCATACACCCCTTATG+TGG 0.553547 5_3:-42343555 Msa0812370:five_prime_UTR
TTGAATCGATTGTGGCTCTG+AGG 0.560216 5_3:+42343420 None:intergenic
GACCTTCAAACACGGTTGAG+AGG 0.564065 5_3:+42343457 None:intergenic
TACCAGTTTAACAAAGAGGA+GGG 0.568079 5_3:-42340865 Msa0812370:CDS
GGGTGTATGGAAGAGTGGTA+TGG 0.569311 5_3:+42343564 None:intergenic
TCAGAAGATTATGCTGTGAT+GGG 0.571791 5_3:-42343143 Msa0812370:five_prime_UTR
ATGGAAGAGTGGTATGGAGA+AGG 0.576479 5_3:+42343570 None:intergenic
CATACACCCCTTATGTGGCA+AGG 0.597889 5_3:-42343550 Msa0812370:five_prime_UTR
GTATTACCAGTTTAACAAAG+AGG 0.598288 5_3:-42340869 Msa0812370:CDS
CCTCTCTTACAGGACAACAC+TGG 0.607683 5_3:-42341290 Msa0812370:intron
TCTTGGAATCATGAAAGTCA+AGG 0.609272 5_3:-42340896 Msa0812370:CDS
TCTCGCAATCCAAGCACGCA+AGG 0.609643 5_3:-42343338 Msa0812370:five_prime_UTR
ATAAGGGGTGTATGGAAGAG+TGG 0.614419 5_3:+42343559 None:intergenic
TTACCAGTTTAACAAAGAGG+AGG 0.617647 5_3:-42340866 Msa0812370:CDS
ATACACCCCTTATGTGGCAA+GGG 0.627096 5_3:-42343549 Msa0812370:five_prime_UTR
AAATACCCTTGCCACATAAG+GGG 0.628337 5_3:+42343544 None:intergenic
TCACGTGCAGGAATATCTTG+AGG 0.635000 5_3:-42341046 Msa0812370:intron
AAGATGAAAAGTGTATGGGA+AGG 0.640402 5_3:-42340823 Msa0812370:CDS
TGTTGACCTTGAATCGATTG+TGG 0.643048 5_3:+42343412 None:intergenic
GAGATTGAGACCTTCAAACA+CGG 0.650252 5_3:+42343449 None:intergenic
AATATGTTGGAACCGTGAAG+AGG 0.650450 5_3:-42341023 Msa0812370:CDS
CATTGGGGAAACATGAAACA+GGG 0.663680 5_3:-42343113 Msa0812370:five_prime_UTR
TATCAACATAAACATTCATG+GGG 0.688765 5_3:+42341195 None:intergenic
GTGATGGGATCAGATCATTG+GGG 0.814520 5_3:-42343128 Msa0812370:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATGGTTTTATATTATT+TGG - chr5_3:42341850-42341869 Msa0812370:intron 10.0%
!! TAAAATTACTTGAAATAAAA+AGG - chr5_3:42342764-42342783 Msa0812370:intron 10.0%
!! TATTTATACAAATATCAAAT+AGG + chr5_3:42342943-42342962 None:intergenic 10.0%
!! TTTAAACTTGAAATTAACAA+TGG + chr5_3:42341244-42341263 None:intergenic 15.0%
!! TATATGAATATGTTGAATTA+TGG - chr5_3:42341835-42341854 Msa0812370:intron 15.0%
!!! ATAAAGGATTATTATTTCTA+TGG + chr5_3:42343001-42343020 None:intergenic 15.0%
!! TAATAATCCTTTATTAGTTA+AGG - chr5_3:42343007-42343026 Msa0812370:intron 15.0%
!! AATAATCCTTTATTAGTTAA+GGG - chr5_3:42343008-42343027 Msa0812370:intron 15.0%
!! AGATTAAATTTAAGAAAAAC+TGG - chr5_3:42343065-42343084 Msa0812370:intron 15.0%
!! TTATTCTATATAGAATTGAA+TGG - chr5_3:42343094-42343113 Msa0812370:five_prime_UTR 15.0%
!! AAGATAAAAGATGAAAATCA+TGG + chr5_3:42341064-42341083 None:intergenic 20.0%
!! CAATGCATAAAAAAGAAAAT+GGG + chr5_3:42341171-42341190 None:intergenic 20.0%
!! TCAATGCATAAAAAAGAAAA+TGG + chr5_3:42341172-42341191 None:intergenic 20.0%
!!! TTGTAGTGTTTCAATTTTAA+GGG - chr5_3:42341504-42341523 Msa0812370:intron 20.0%
!! TGTGATTATGAATCTATTAT+AGG + chr5_3:42341661-42341680 None:intergenic 20.0%
!! AATAGATTCATAATCACAAT+TGG - chr5_3:42341663-42341682 Msa0812370:intron 20.0%
!!! TTTTAAAACTCAACATATGA+TGG + chr5_3:42341761-42341780 None:intergenic 20.0%
!!! TTGAGTTTTAAAATTTTCTG+AGG - chr5_3:42341768-42341787 Msa0812370:intron 20.0%
!!! TGGTTTTATATTATTTGGAT+AGG - chr5_3:42341855-42341874 Msa0812370:intron 20.0%
!!! AAAAAAAGAATTTTGAGAAC+GGG + chr5_3:42341904-42341923 None:intergenic 20.0%
!!! GAAAAAAAGAATTTTGAGAA+CGG + chr5_3:42341905-42341924 None:intergenic 20.0%
!!! TCATAGTTATTTTATTGAGA+AGG + chr5_3:42342703-42342722 None:intergenic 20.0%
!! ATATAGAATTGAATGGTAAA+AGG - chr5_3:42343101-42343120 Msa0812370:five_prime_UTR 20.0%
!! ATATCAACATAAACATTCAT+GGG + chr5_3:42343235-42343254 None:intergenic 20.0%
!! TCATGTTAACATAAAAGATT+AGG - chr5_3:42343298-42343317 Msa0812370:five_prime_UTR 20.0%
! TAATGTGTATGGAAAAAGAT+TGG - chr5_3:42340826-42340845 Msa0812370:CDS 25.0%
!! CTTGTAGTGTTTCAATTTTA+AGG - chr5_3:42341503-42341522 Msa0812370:intron 25.0%
!! AGTTATGTCTTTTAGCAATT+AGG - chr5_3:42341535-42341554 Msa0812370:intron 25.0%
! AGTTATATAGTCATGTCATT+AGG - chr5_3:42341604-42341623 Msa0812370:intron 25.0%
! TATGAATCTATTATAGGCAA+AGG + chr5_3:42341655-42341674 None:intergenic 25.0%
! ATTCATAATCACAATTGGTT+TGG - chr5_3:42341668-42341687 Msa0812370:intron 25.0%
! ATGTAAAGCTCAAACTATAA+TGG + chr5_3:42341708-42341727 None:intergenic 25.0%
!!! AAATTTTCTGAGGATGATTT+AGG - chr5_3:42341778-42341797 Msa0812370:intron 25.0%
!!! AATTTTCTGAGGATGATTTA+GGG - chr5_3:42341779-42341798 Msa0812370:intron 25.0%
!!! AAAAAAGAATTTTGAGAACG+GGG + chr5_3:42341903-42341922 None:intergenic 25.0%
! AAGTAAAGACTAAAGAAAAC+AGG + chr5_3:42342004-42342023 None:intergenic 25.0%
!!! TCTACTTTGTTTCTAAACTT+TGG - chr5_3:42342156-42342175 Msa0812370:intron 25.0%
!! TTTTTATGAGTGAGATTTCT+AGG - chr5_3:42342504-42342523 Msa0812370:intron 25.0%
!! GATTTTTGTTGAATGTTGTT+TGG - chr5_3:42342797-42342816 Msa0812370:intron 25.0%
!!! ACACAACTATTGATTTTTCT+AGG - chr5_3:42342876-42342895 Msa0812370:intron 25.0%
! TATCAACATAAACATTCATG+GGG + chr5_3:42343234-42343253 None:intergenic 25.0%
! CATATCAACATAAACATTCA+TGG + chr5_3:42343236-42343255 None:intergenic 25.0%
!! AAATTTTATATATCACGTGC+AGG - chr5_3:42343368-42343387 Msa0812370:five_prime_UTR 25.0%
!! AAGAAAAGATGAAAAGTGTA+TGG - chr5_3:42343598-42343617 Msa0812370:five_prime_UTR 25.0%
!! AGAAAAGATGAAAAGTGTAT+GGG - chr5_3:42343599-42343618 Msa0812370:five_prime_UTR 25.0%
! ACAAGATTTTCGGTAAGATT+CGG - chr5_3:42341142-42341161 Msa0812370:intron 30.0%
AAATGTAAGCGTATTGAGTT+GGG - chr5_3:42341364-42341383 Msa0812370:intron 30.0%
AACAGGAACAGAAAAATACA+GGG + chr5_3:42341416-42341435 None:intergenic 30.0%
AAACAGGAACAGAAAAATAC+AGG + chr5_3:42341417-42341436 None:intergenic 30.0%
ATCAAATTAGCGAACAAAAC+AGG + chr5_3:42341433-42341452 None:intergenic 30.0%
AATATCTGTGCTGAAGAAAA+AGG + chr5_3:42341465-42341484 None:intergenic 30.0%
AGCACAGATATTTAGTAGTT+GGG - chr5_3:42341473-42341492 Msa0812370:intron 30.0%
! CTATAATGGAGTTCATAGAA+TGG + chr5_3:42341694-42341713 None:intergenic 30.0%
!!! TCTTTTTTTCTGTGTAGTGT+TGG - chr5_3:42341915-42341934 Msa0812370:intron 30.0%
! CGTTCATAACACGTTTTATA+GGG + chr5_3:42341947-42341966 None:intergenic 30.0%
! TCGTTCATAACACGTTTTAT+AGG + chr5_3:42341948-42341967 None:intergenic 30.0%
! CGTTCATAACACGTTTTATA+GGG + chr5_3:42341947-42341966 None:intergenic 30.0%
! TCGTTCATAACACGTTTTAT+AGG + chr5_3:42341948-42341967 None:intergenic 30.0%
GCTTAGAGTAATGTAAAACA+TGG - chr5_3:42342039-42342058 Msa0812370:intron 30.0%
! ATTTGATAATATTGCCTGCA+AGG + chr5_3:42342082-42342101 None:intergenic 30.0%
GTCAAAATTGTGTATCTTTC+AGG - chr5_3:42342203-42342222 Msa0812370:intron 30.0%
GCTTATAGTAATACTAAGCA+TGG - chr5_3:42342322-42342341 Msa0812370:intron 30.0%
ATTGACAAAAACTACATGCA+AGG + chr5_3:42342368-42342387 None:intergenic 30.0%
! CATGTAGTTTTTGTCAATCA+TGG - chr5_3:42342370-42342389 Msa0812370:intron 30.0%
! TATTTGGTAGTATACAACTG+TGG - chr5_3:42342443-42342462 Msa0812370:intron 30.0%
TTTCACTTTCAATGTACTCT+TGG - chr5_3:42342622-42342641 Msa0812370:intron 30.0%
! ATTGAGAAGGTTTTCCTTTA+CGG + chr5_3:42342690-42342709 None:intergenic 30.0%
TGTGTTAATGTGCTATCTTT+AGG - chr5_3:42343330-42343349 Msa0812370:five_prime_UTR 30.0%
GAATATCTTGAGGAATATGT+TGG - chr5_3:42343390-42343409 Msa0812370:five_prime_UTR 30.0%
CTGAAAAGAAGCTTCAATTT+AGG - chr5_3:42343456-42343475 Msa0812370:five_prime_UTR 30.0%
TCTTTCTTATCTTTCACCTT+GGG + chr5_3:42343510-42343529 None:intergenic 30.0%
ATCTTTCTTATCTTTCACCT+TGG + chr5_3:42343511-42343530 None:intergenic 30.0%
GTGAAAGATAAGAAAGATCT+TGG - chr5_3:42343513-42343532 Msa0812370:five_prime_UTR 30.0%
GTATTACCAGTTTAACAAAG+AGG - chr5_3:42343557-42343576 Msa0812370:five_prime_UTR 30.0%
AAAATACCCTTGCCACATAA+GGG + chr5_3:42340886-42340905 None:intergenic 35.0%
AAAAATACCCTTGCCACATA+AGG + chr5_3:42340887-42340906 None:intergenic 35.0%
! ATCAGCTACGACAAGATTTT+CGG - chr5_3:42341132-42341151 Msa0812370:intron 35.0%
TTCAGAAGATTATGCTGTGA+TGG - chr5_3:42341282-42341301 Msa0812370:intron 35.0%
TCAGAAGATTATGCTGTGAT+GGG - chr5_3:42341283-42341302 Msa0812370:intron 35.0%
ACTCAATACGCTTACATTTG+TGG + chr5_3:42341363-42341382 None:intergenic 35.0%
CAAATGTAAGCGTATTGAGT+TGG - chr5_3:42341363-42341382 Msa0812370:intron 35.0%
CAGCACAGATATTTAGTAGT+TGG - chr5_3:42341472-42341491 Msa0812370:intron 35.0%
ATAGTCATGTCATTAGGTCA+TGG - chr5_3:42341610-42341629 Msa0812370:intron 35.0%
TAGTCATGTCATTAGGTCAT+GGG - chr5_3:42341611-42341630 Msa0812370:intron 35.0%
ATGGCATAAGTTCTTGGAAA+AGG + chr5_3:42341742-42341761 None:intergenic 35.0%
CATATGATGGCATAAGTTCT+TGG + chr5_3:42341748-42341767 None:intergenic 35.0%
! TAACACGTTTTATAGGGAAG+TGG + chr5_3:42341941-42341960 None:intergenic 35.0%
TCAAAATTGTGTTTCCTTGC+AGG - chr5_3:42342065-42342084 Msa0812370:intron 35.0%
CAAAGTAGACTAAAGAGAAC+AGG + chr5_3:42342145-42342164 None:intergenic 35.0%
GTGTACAATAATGTGAAGCA+TGG - chr5_3:42342178-42342197 Msa0812370:intron 35.0%
AAGTAAAGACTGAAGTGAAC+AGG + chr5_3:42342289-42342308 None:intergenic 35.0%
AATTATATGCCGCTGCTATT+TGG - chr5_3:42342427-42342446 Msa0812370:intron 35.0%
TGTATACTACCAAATAGCAG+CGG + chr5_3:42342439-42342458 None:intergenic 35.0%
TCTTGGATTTGTGTATCTAG+AGG - chr5_3:42342639-42342658 Msa0812370:intron 35.0%
TGTTTGGATTGTTTGCGATT+TGG - chr5_3:42342813-42342832 Msa0812370:intron 35.0%
CTCACACCCTTAACTAATAA+AGG + chr5_3:42343017-42343036 None:intergenic 35.0%
! TTATTAGTTAAGGGTGTGAG+AGG - chr5_3:42343017-42343036 Msa0812370:intron 35.0%
AGAGGTCATACAATCTTACT+TGG - chr5_3:42343035-42343054 Msa0812370:intron 35.0%
! TCAACTTTTGTGAGTGAAGT+TGG - chr5_3:42343181-42343200 Msa0812370:intron 35.0%
GTACTGTACCTGATACAATA+TGG + chr5_3:42343433-42343452 None:intergenic 35.0%
TCTTGGAATCATGAAAGTCA+AGG - chr5_3:42343530-42343549 Msa0812370:five_prime_UTR 35.0%
TTACCAGTTTAACAAAGAGG+AGG - chr5_3:42343560-42343579 Msa0812370:five_prime_UTR 35.0%
TACCAGTTTAACAAAGAGGA+GGG - chr5_3:42343561-42343580 Msa0812370:five_prime_UTR 35.0%
! AAGATGAAAAGTGTATGGGA+AGG - chr5_3:42343603-42343622 Msa0812370:five_prime_UTR 35.0%
AAATACCCTTGCCACATAAG+GGG + chr5_3:42340885-42340904 None:intergenic 40.0%
GAGATCTAAGAGAGAGAAGA+GGG + chr5_3:42340938-42340957 None:intergenic 40.0%
GAGATTGAGACCTTCAAACA+CGG + chr5_3:42340980-42340999 None:intergenic 40.0%
TGTTGACCTTGAATCGATTG+TGG + chr5_3:42341017-42341036 None:intergenic 40.0%
! TGTGATGGGATCAGATCATT+GGG - chr5_3:42341297-42341316 Msa0812370:intron 40.0%
TCATTGGGGAAACATGAAAC+AGG - chr5_3:42341312-42341331 Msa0812370:intron 40.0%
CATTGGGGAAACATGAAACA+GGG - chr5_3:42341313-42341332 Msa0812370:intron 40.0%
!! CAATTAGGCTGTTTTCTTCC+AGG - chr5_3:42341550-42341569 Msa0812370:intron 40.0%
TATAACTCTAACCATTGCGC+TGG + chr5_3:42341591-42341610 None:intergenic 40.0%
!! TCAGGTAGCATTGACAATTG+CGG - chr5_3:42342221-42342240 Msa0812370:intron 40.0%
!!! TTCGTTGCTTTTTTGCTCTG+TGG - chr5_3:42342548-42342567 Msa0812370:intron 40.0%
AAAAACAACCCATCAACTGG+AGG + chr5_3:42342605-42342624 None:intergenic 40.0%
GTGAAAAACAACCCATCAAC+TGG + chr5_3:42342608-42342627 None:intergenic 40.0%
CTAGAGGAATACATATCTCG+TGG - chr5_3:42342655-42342674 Msa0812370:intron 40.0%
AAGCACGAACCAGAGAAATT+GGG - chr5_3:42342722-42342741 Msa0812370:intron 40.0%
ACGACAAAACCCAATTTCTC+TGG + chr5_3:42342734-42342753 None:intergenic 40.0%
!! GAGAAATTGGGTTTTGTCGT+CGG - chr5_3:42342734-42342753 Msa0812370:intron 40.0%
!! AGAAATTGGGTTTTGTCGTC+GGG - chr5_3:42342735-42342754 Msa0812370:intron 40.0%
! GTTACTTTCACCTCTCTTAC+AGG - chr5_3:42343126-42343145 Msa0812370:five_prime_UTR 40.0%
AACACTGGAAAGTTGGCTAA+TGG - chr5_3:42343151-42343170 Msa0812370:five_prime_UTR 40.0%
! ACTTTTGTGAGTGAAGTTGG+AGG - chr5_3:42343184-42343203 Msa0812370:intron 40.0%
! CTTTTGTGAGTGAAGTTGGA+GGG - chr5_3:42343185-42343204 Msa0812370:intron 40.0%
! TTTTGTGAGTGAAGTTGGAG+GGG - chr5_3:42343186-42343205 Msa0812370:intron 40.0%
AATATGTTGGAACCGTGAAG+AGG - chr5_3:42343403-42343422 Msa0812370:five_prime_UTR 40.0%
CAATATGGTTCTCCTCTTCA+CGG + chr5_3:42343418-42343437 None:intergenic 40.0%
GAGGAGAACCATATTGTATC+AGG - chr5_3:42343422-42343441 Msa0812370:five_prime_UTR 40.0%
GTCATGACTTGATATACCCA+AGG - chr5_3:42343491-42343510 Msa0812370:five_prime_UTR 40.0%
ATCCCTCCTCTTTGTTAAAC+TGG + chr5_3:42343566-42343585 None:intergenic 40.0%
GTTTAACAAAGAGGAGGGAT+TGG - chr5_3:42343566-42343585 Msa0812370:five_prime_UTR 40.0%
TGGAAGAGTGGTATGGAGAA+GGG + chr5_3:42340858-42340877 None:intergenic 45.0%
ATGGAAGAGTGGTATGGAGA+AGG + chr5_3:42340859-42340878 None:intergenic 45.0%
! ATAAGGGGTGTATGGAAGAG+TGG + chr5_3:42340870-42340889 None:intergenic 45.0%
TCTTCCATACACCCCTTATG+TGG - chr5_3:42340871-42340890 Msa0812370:CDS 45.0%
ATACACCCCTTATGTGGCAA+GGG - chr5_3:42340877-42340896 Msa0812370:CDS 45.0%
GGAGATCTAAGAGAGAGAAG+AGG + chr5_3:42340939-42340958 None:intergenic 45.0%
ACCTTCAAACACGGTTGAGA+GGG + chr5_3:42340971-42340990 None:intergenic 45.0%
!! TGAATCGATTGTGGCTCTGA+GGG + chr5_3:42341008-42341027 None:intergenic 45.0%
! TTGAATCGATTGTGGCTCTG+AGG + chr5_3:42341009-42341028 None:intergenic 45.0%
! TCAGAGCCACAATCGATTCA+AGG - chr5_3:42341008-42341027 Msa0812370:CDS 45.0%
! CTGTGATGGGATCAGATCAT+TGG - chr5_3:42341296-42341315 Msa0812370:intron 45.0%
! GTGATGGGATCAGATCATTG+GGG - chr5_3:42341298-42341317 Msa0812370:intron 45.0%
CGACTCACAAACTTCATGTC+TGG + chr5_3:42341393-42341412 None:intergenic 45.0%
!! TAGGCTGTTTTCTTCCAGGA+AGG - chr5_3:42341554-42341573 Msa0812370:intron 45.0%
!! AGGCTGTTTTCTTCCAGGAA+GGG - chr5_3:42341555-42341574 Msa0812370:intron 45.0%
TAGGTCATGGGTTTCAGTCA+TGG - chr5_3:42341623-42341642 Msa0812370:intron 45.0%
!!! TTGCTTTTTTGCTCTGTGGC+AGG - chr5_3:42342552-42342571 Msa0812370:intron 45.0%
! TAGCCTTTTCCTCCAGTTGA+TGG - chr5_3:42342593-42342612 Msa0812370:intron 45.0%
! AGCCTTTTCCTCCAGTTGAT+GGG - chr5_3:42342594-42342613 Msa0812370:intron 45.0%
AACCCATCAACTGGAGGAAA+AGG + chr5_3:42342599-42342618 None:intergenic 45.0%
GAAGCACGAACCAGAGAAAT+TGG - chr5_3:42342721-42342740 Msa0812370:intron 45.0%
ACAGGACAACACTGGAAAGT+TGG - chr5_3:42343144-42343163 Msa0812370:five_prime_UTR 45.0%
TCACGTGCAGGAATATCTTG+AGG - chr5_3:42343380-42343399 Msa0812370:five_prime_UTR 45.0%
GGGTGTATGGAAGAGTGGTA+TGG + chr5_3:42340865-42340884 None:intergenic 50.0%
! CTTGCCACATAAGGGGTGTA+TGG + chr5_3:42340878-42340897 None:intergenic 50.0%
CATACACCCCTTATGTGGCA+AGG - chr5_3:42340876-42340895 Msa0812370:CDS 50.0%
CGGTTGAGAGGGAGAAGAAA+GGG + chr5_3:42340960-42340979 None:intergenic 50.0%
ACGGTTGAGAGGGAGAAGAA+AGG + chr5_3:42340961-42340980 None:intergenic 50.0%
TCCCTCTCAACCGTGTTTGA+AGG - chr5_3:42340967-42340986 Msa0812370:intron 50.0%
GACCTTCAAACACGGTTGAG+AGG + chr5_3:42340972-42340991 None:intergenic 50.0%
ATCCAAGCACGCAAGGTTCT+AGG - chr5_3:42341095-42341114 Msa0812370:intron 50.0%
TCCAAGCACGCAAGGTTCTA+GGG - chr5_3:42341096-42341115 Msa0812370:intron 50.0%
!! GGCTGTTTTCTTCCAGGAAG+GGG - chr5_3:42341556-42341575 Msa0812370:intron 50.0%
CGTGGATTTGCGAACCGTAA+AGG - chr5_3:42342673-42342692 Msa0812370:intron 50.0%
CCAGTGTTGTCCTGTAAGAG+AGG + chr5_3:42343139-42343158 None:intergenic 50.0%
CCTCTCTTACAGGACAACAC+TGG - chr5_3:42343136-42343155 Msa0812370:five_prime_UTR 50.0%
GGGGCTGTAATCCATGGAAA+TGG - chr5_3:42343205-42343224 Msa0812370:intron 50.0%
TCATGGGGAGACCATTTCCA+TGG + chr5_3:42343219-42343238 None:intergenic 50.0%
TCTCGCAATCCAAGCACGCA+AGG - chr5_3:42341088-42341107 Msa0812370:intron 55.0%
ACCCTAGAACCTTGCGTGCT+TGG + chr5_3:42341100-42341119 None:intergenic 55.0%
TTCTTCCAGGAAGGGGACCT+CGG - chr5_3:42341563-42341582 Msa0812370:intron 55.0%
GTTGGAGGGGCTGTAATCCA+TGG - chr5_3:42343199-42343218 Msa0812370:intron 55.0%
! TAACCATTGCGCTGGCACCG+AGG + chr5_3:42341583-42341602 None:intergenic 60.0%
! TGGCACCGAGGTCCCCTTCC+TGG + chr5_3:42341571-42341590 None:intergenic 70.0%
!! GGACCTCGGTGCCAGCGCAA+TGG - chr5_3:42341577-42341596 Msa0812370:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 42340816 42343632 42340816 ID=Msa0812370;Name=Msa0812370
chr5_3 mRNA 42340816 42343632 42340816 ID=Msa0812370-mRNA-1;Parent=Msa0812370;Name=Msa0812370-mRNA-1;_AED=0.20;_eAED=0.20;_QI=524|0.75|0.6|1|0.5|0.6|5|0|75
chr5_3 exon 42340816 42340935 42340816 ID=Msa0812370-mRNA-1:exon:13023;Parent=Msa0812370-mRNA-1
chr5_3 exon 42341005 42341058 42341005 ID=Msa0812370-mRNA-1:exon:13022;Parent=Msa0812370-mRNA-1
chr5_3 exon 42341160 42341300 42341160 ID=Msa0812370-mRNA-1:exon:13021;Parent=Msa0812370-mRNA-1
chr5_3 exon 42343081 42343179 42343081 ID=Msa0812370-mRNA-1:exon:13020;Parent=Msa0812370-mRNA-1
chr5_3 exon 42343295 42343632 42343295 ID=Msa0812370-mRNA-1:exon:13019;Parent=Msa0812370-mRNA-1
chr5_3 five_prime_UTR 42343295 42343632 42343295 ID=Msa0812370-mRNA-1:five_prime_utr;Parent=Msa0812370-mRNA-1
chr5_3 five_prime_UTR 42343081 42343179 42343081 ID=Msa0812370-mRNA-1:five_prime_utr;Parent=Msa0812370-mRNA-1
chr5_3 five_prime_UTR 42341214 42341300 42341214 ID=Msa0812370-mRNA-1:five_prime_utr;Parent=Msa0812370-mRNA-1
chr5_3 CDS 42341160 42341213 42341160 ID=Msa0812370-mRNA-1:cds;Parent=Msa0812370-mRNA-1
chr5_3 CDS 42341005 42341058 42341005 ID=Msa0812370-mRNA-1:cds;Parent=Msa0812370-mRNA-1
chr5_3 CDS 42340816 42340935 42340816 ID=Msa0812370-mRNA-1:cds;Parent=Msa0812370-mRNA-1
Gene Sequence

>Msa0812370

ATGAATGTTTATGTTGATATGTCTATTTCTTTTCTTGCAACTGCTCATGTTAGTGAATATCTTGAGGAATATGTTGGAACCGTGAAGAGGAGAACCATATTGTATCAGGTGAAAGATAAGAAAGATCTTGGAATCATGAAAGTCAAGGTCTTGTATTACCAGTTTAACAAAGAGGAGGGATTGGTTGTACTAGAAGAAAAGATGAAAAGTGTATGGGAAGGACCATAA
Protein sequence

>Msa0812370

MNVYVDMSISFLATAHVSEYLEEYVGTVKRRTILYQVKDKKDLGIMKVKVLYYQFNKEEGLVVLEEKMKSVWEGP*