AlfalfaGEDB | Msa0817280

M. sativa cultivar zhongmu-4 / Msa0817280

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
PFAM: PF00412.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817280	sp\|Q0WSN2\|DAR2_ARATH	64.648	512	149	9	8	491	21	528	0.0	639

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817280	A0A396HX06	95.833	504	8	1	1	491	113	616	0.0	1013

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TFs/TRs:

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Gene ID	Type	Classification
Msa0817280	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0737810	Msa0817280	0.995603	6.011506e-218	-8.615850e-47
Msa0785440	Msa0817280	0.818580	1.819846e-52	-8.615850e-47
Msa0817250	Msa0817280	0.914387	2.034682e-84	-8.615850e-47
Msa0817280	Msa0857540	0.800365	1.491583e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817280	MtrunA17_Chr5g0436051	95.833	504	8	1	1	491	113	616	0.0	1013
Msa0817280	MtrunA17_Chr3g0112601	70.986	517	120	6	1	491	1	513	0.0	739
Msa0817280	MtrunA17_Chr1g0154501	50.731	479	205	8	32	490	1	468	3.53e-166	478
Msa0817280	MtrunA17_Chr8g0373781	49.813	536	187	10	32	490	1	531	7.26e-165	476
Msa0817280	MtrunA17_Chr5g0403301	56.357	291	99	3	207	490	37	306	3.80e-109	326
Msa0817280	MtrunA17_Chr5g0403261	37.228	368	137	8	32	307	1	366	2.95e-74	238
Msa0817280	MtrunA17_Chr5g0403271	64.706	153	49	1	343	490	1	153	1.58e-59	192
Msa0817280	MtrunA17_Chr5g0403291	57.576	66	28	0	141	206	382	447	1.48e-20	94.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817280	AT2G39830.1	64.648	512	149	9	8	491	21	528	0.0	639
Msa0817280	AT2G39830.2	64.272	515	149	10	8	491	21	531	0.0	634
Msa0817280	AT2G39830.3	64.244	509	147	9	8	491	21	519	0.0	630
Msa0817280	AT2G39830.4	62.175	423	132	8	8	406	21	439	5.94e-179	509
Msa0817280	AT4G36860.1	59.145	421	144	5	92	490	139	553	2.30e-168	486
Msa0817280	AT4G36860.3	59.145	421	144	5	92	490	163	577	2.79e-168	487
Msa0817280	AT1G19270.1	46.154	533	213	7	32	491	1	532	1.00e-160	466
Msa0817280	AT1G19270.2	46.154	533	213	7	32	491	1	532	1.00e-160	466
Msa0817280	AT4G36860.2	58.075	322	109	4	92	393	139	454	6.40e-127	377
Msa0817280	AT5G66610.3	50.847	354	161	5	142	484	73	424	4.82e-115	346

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Find 127 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACTATAGACTCGAGGA+CGG	0.654015	5_3:-52274695	Msa0817280:CDS
AAATGATGAAGATTATGGAA+AGG	0.584158	5_3:-52275962	Msa0817280:CDS
AACAAGAGGTTTATGCCTTT+CGG	0.351720	5_3:-52274393	Msa0817280:CDS
AACATGTGTTCTTATTACAC+AGG	0.561919	5_3:+52273496	None:intergenic
AACCTCTTGTTTCTGGCAAA+TGG	0.381781	5_3:+52274405	None:intergenic
AACTACCTCGCGCTCGGTAA+TGG	0.338884	5_3:+52275672	None:intergenic
AACTTCTATATGGTCCTGTG+AGG	0.664585	5_3:-52273346	Msa0817280:three_prime_UTR
AAGATTTCACCTGTATTCAT+TGG	0.335163	5_3:+52275879	None:intergenic
AAGCTTGTGCTTAGAGTGCA+TGG	0.547235	5_3:-52274669	Msa0817280:CDS
AAGGTGGGTCCAATAGAGGA+AGG	0.645391	5_3:-52276919	Msa0817280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATTGATATGATCAGAAGA+AGG	+	chr5_3:52273308-52273327	None:intergenic	30.0%
CTTTCTCATCCATGTATATA+TGG	-	chr5_3:52273329-52273348	Msa0817280:three_prime_UTR	30.0%
AGAAAAAGAGAAAAGAGAGA+AGG	+	chr5_3:52273366-52273385	None:intergenic	30.0%
GTTGGAGTAAAATTGTGTAA+AGG	+	chr5_3:52273449-52273468	None:intergenic	30.0%
TTTGATGTTTGTGTTTGTGA+CGG	+	chr5_3:52273663-52273682	None:intergenic	30.0%
ACAAACACAAACATCAAACA+TGG	-	chr5_3:52273665-52273684	Msa0817280:CDS	30.0%
TGTTGGAACTTATTGAATGT+TGG	+	chr5_3:52274180-52274199	None:intergenic	30.0%
CTAAAAAATCACACACATGT+TGG	+	chr5_3:52274197-52274216	None:intergenic	30.0%
TTCATCTTGATGAGATCAAT+AGG	+	chr5_3:52274477-52274496	None:intergenic	30.0%
GGAGAAAATGATGAAGATTA+TGG	-	chr5_3:52274881-52274900	Msa0817280:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	52273231	52277639	52273231	ID=Msa0817280;Name=Msa0817280
chr5_3	mRNA	52273231	52277639	52273231	ID=Msa0817280-mRNA-1;Parent=Msa0817280;Name=Msa0817280-mRNA-1;_AED=0.13;_eAED=0.13;_QI=68\|0.90\|0.83\|1\|0.81\|0.83\|12\|279\|491
chr5_3	exon	52273231	52273826	52273231	ID=Msa0817280-mRNA-1:exon:15722;Parent=Msa0817280-mRNA-1
chr5_3	exon	52273914	52273972	52273914	ID=Msa0817280-mRNA-1:exon:15721;Parent=Msa0817280-mRNA-1
chr5_3	exon	52274141	52274256	52274141	ID=Msa0817280-mRNA-1:exon:15720;Parent=Msa0817280-mRNA-1
chr5_3	exon	52274373	52274435	52274373	ID=Msa0817280-mRNA-1:exon:15719;Parent=Msa0817280-mRNA-1
chr5_3	exon	52274517	52274729	52274517	ID=Msa0817280-mRNA-1:exon:15718;Parent=Msa0817280-mRNA-1
chr5_3	exon	52274843	52274927	52274843	ID=Msa0817280-mRNA-1:exon:15717;Parent=Msa0817280-mRNA-1
chr5_3	exon	52275506	52275585	52275506	ID=Msa0817280-mRNA-1:exon:15716;Parent=Msa0817280-mRNA-1
chr5_3	exon	52275678	52275801	52275678	ID=Msa0817280-mRNA-1:exon:15715;Parent=Msa0817280-mRNA-1
chr5_3	exon	52275889	52276012	52275889	ID=Msa0817280-mRNA-1:exon:15714;Parent=Msa0817280-mRNA-1
chr5_3	exon	52276109	52276196	52276109	ID=Msa0817280-mRNA-1:exon:15713;Parent=Msa0817280-mRNA-1
chr5_3	exon	52276853	52277007	52276853	ID=Msa0817280-mRNA-1:exon:15712;Parent=Msa0817280-mRNA-1
chr5_3	exon	52277520	52277639	52277520	ID=Msa0817280-mRNA-1:exon:15711;Parent=Msa0817280-mRNA-1
chr5_3	five_prime_UTR	52277572	52277639	52277572	ID=Msa0817280-mRNA-1:five_prime_utr;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52277520	52277571	52277520	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52276853	52277007	52276853	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52276109	52276196	52276109	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52275889	52276012	52275889	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52275678	52275801	52275678	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52275506	52275585	52275506	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52274843	52274927	52274843	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52274517	52274729	52274517	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52274373	52274435	52274373	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52274141	52274256	52274141	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52273914	52273972	52273914	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	CDS	52273510	52273826	52273510	ID=Msa0817280-mRNA-1:cds;Parent=Msa0817280-mRNA-1
chr5_3	three_prime_UTR	52273231	52273509	52273231	ID=Msa0817280-mRNA-1:three_prime_utr;Parent=Msa0817280-mRNA-1

Gene Sequence

>Msa0817280

ATGGCTCCTTCTTCTGATCATATCAATCATCTTTCTCATCCATGTATATATGGGGATTATGTGTCTTCACACCCAGAGAGAAAGTCTGGTTTCATGAAATGGCTTAGTAAGCTTTTCAAAGGTGGGTCCAATAGAGGAAGGAGTGGTCGTCATCATTATGATCCTGCAGAGGAAGGCATGTCTTGGCGTGCTCCATCTAGAGCTTTGGATGATCGTGCTAGAGCTCAGAAGGAGAAAGAGGATTTAGGCCATGCAATGTCACTTTCTTCAGCAGAAGATATGAAGAGACCAAATGCCCATCAAGGATATAGGTGGGGGGGAGAAAATGATGAAGATTATGGAAAGGCACTTCATGATAACTTTAACTCATCTGCACACCCTCCTTATGCTCCTGCACCATTTTATCCCAATGAATACAGAAGACTATGTGGGGGCTGCAATCAGGAGATAATCTATGGAAATTGTTTAGGCTGCATGGATACTTATTTTCATCCAGACTGCTTTCGATGCCACTCTTGTCATTCTCCCATTACCGAGCGCGAGTTTTCTTTGTCAGGGAAGCATCCATATCACAAATCTTGTTTTAAAGAGTTGACTCACCCAAAATGTGAAGTTTGCTTCCAATGCCACCCCTTTTGGTCCCAAAAGTATTGTCCATCTCATGAATATGACAATACAGCTCGCTGCTGTAGTTGTGAAAGATTAGAGCCTCGGAATACGAAATACTATAGACTCGAGGACGGGCGAAGCTTGTGCTTAGAGTGCATGGAATCTGCTATAATGGATACTGGTGACTGTCAGCCTCTATACCATTCAATCAGAGATTATTATGAAGGAATGCATATGAAAATTGATCAGCAAGTTCCCATGCTTCTTGTTGAAAGAGAAGCACTTAATGATGCTATTGTTGGAGAGAAGACTGGATTTCACCATTTGCCAGAAACAAGAGGTTTATGCCTTTCGGAAGAGCAAACTGTGACCAGTATTCATAGAAGGCCAAGAATTGGAGGCCATAGACTTATAGGCATGAGAACTCAACCTCAAAAGCTAATAAGAAAATGTGAAGTTACAGCAATTCTTGTTCTATATGGTCTACCAAGGTTACTAACAGGTGCTATACTTGCCCATGAGTTGATGCATGCATGGTTACGCCTTAAAGGTTATCGCAATCTCAGTCCTGAAGTAGAGGAAGGCATATGTCAGGTTCTGTCATATATGTGGCTTGAGTCAGAAGTTATGCCAGCAACAAACTCTCATTGCATGGCATCAACATCCACAGCTGCTGCTTCTTCCTCAAAGAAAGGTGCAAAGTCTCATGTTGAAAACAAATTGGGTGAATTCTTCAAGAATCAAATTGTCAATGATTCTTCTCCAGCTTATGGTGGTGGTTTTAGAGCTGCAAATGAAGCTGTTAATAAATATGGTCTACGTAGTACTCTCGAACATATTCGTTTGACTGGTTTTATCCCTGTGTAA

Protein sequence

>Msa0817280

MAPSSDHINHLSHPCIYGDYVSSHPERKSGFMKWLSKLFKGGSNRGRSGRHHYDPAEEGMSWRAPSRALDDRARAQKEKEDLGHAMSLSSAEDMKRPNAHQGYRWGGENDEDYGKALHDNFNSSAHPPYAPAPFYPNEYRRLCGGCNQEIIYGNCLGCMDTYFHPDCFRCHSCHSPITEREFSLSGKHPYHKSCFKELTHPKCEVCFQCHPFWSQKYCPSHEYDNTARCCSCERLEPRNTKYYRLEDGRSLCLECMESAIMDTGDCQPLYHSIRDYYEGMHMKIDQQVPMLLVEREALNDAIVGEKTGFHHLPETRGLCLSEEQTVTSIHRRPRIGGHRLIGMRTQPQKLIRKCEVTAILVLYGLPRLLTGAILAHELMHAWLRLKGYRNLSPEVEEGICQVLSYMWLESEVMPATNSHCMASTSTAAASSSKKGAKSHVENKLGEFFKNQIVNDSSPAYGGGFRAANEAVNKYGLRSTLEHIRLTGFIPV*