AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0821170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821170 MtrunA17_Chr5g0441381 95.833 240 9 1 1 239 1 240 4.82e-171 470
Msa0821170 MtrunA17_Chr7g0246301 40.940 149 78 5 9 155 8 148 5.61e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0821170 AT1G08500.1 59.278 194 73 3 13 200 7 200 9.33e-78 234

Find 57 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTATGTTCCATTATTCT+TGG 0.181685 5_3:-59151684 None:intergenic
AAACCGACATGAAATGAAAT+AGG 0.222292 5_3:+59151479 Msa0821170:five_prime_UTR
TCCCAAGCTGAATTCTTTCT+TGG 0.237475 5_3:-59151862 None:intergenic
TCTCCGGCTAGTCCGATTTC+TGG 0.249790 5_3:+59153242 Msa0821170:CDS
AAATTTCACAACATGTTATA+TGG 0.271985 5_3:+59153401 Msa0821170:CDS
TTCCTTAGGATGGTTGAAAT+TGG 0.295594 5_3:-59153300 None:intergenic
CAACTGTTAATTATCAGAAA+TGG 0.300214 5_3:+59151835 Msa0821170:CDS
TTCTATGTTCCATTATTCTT+GGG 0.311538 5_3:-59151683 None:intergenic
TTTCGCTCACCCAAGAATAA+TGG 0.378679 5_3:+59151674 Msa0821170:exon
TCATCGTCACTGTCTTCCTT+AGG 0.390911 5_3:-59153314 None:intergenic
TCATGTTTCTTAGGTTCTGT+TGG 0.394187 5_3:+59151753 Msa0821170:CDS
TACACAGTGGGAGATTCTTT+AGG 0.400880 5_3:+59151789 Msa0821170:CDS
CTTTAACATCGAAACAGAAC+TGG 0.404508 5_3:-59153372 None:intergenic
AACTGTTAATTATCAGAAAT+GGG 0.420262 5_3:+59151836 Msa0821170:CDS
AAAGGAACTGCCACTGTGAC+TGG 0.433267 5_3:-59153080 None:intergenic
TTATACAACAATAACATAGA+TGG 0.440206 5_3:-59153539 None:intergenic
AACCGACATGAAATGAAATA+GGG 0.467134 5_3:+59151480 Msa0821170:five_prime_UTR
GTTGCCCCTTCTTTCAGTAA+AGG 0.467797 5_3:-59153098 None:intergenic
GACTCATCGTCACCAGAAAT+CGG 0.470529 5_3:-59153254 None:intergenic
GCAGTTCCTTTACTGAAAGA+AGG 0.475413 5_3:+59153092 Msa0821170:CDS
GGTGAGCGAAATGTGAATAA+TGG 0.480913 5_3:-59151662 None:intergenic
AAATCACATGTAACCATAGC+TGG 0.482015 5_3:+59151553 Msa0821170:five_prime_UTR
TGACAAAGATAAAGATAAAG+AGG 0.482717 5_3:+59153346 Msa0821170:CDS
GCCAAGAAAGAATTCAGCTT+GGG 0.483597 5_3:+59151861 Msa0821170:CDS
TTGGAAGGAATATTTGTGTC+TGG 0.492247 5_3:-59153281 None:intergenic
GATTCATATGCTGCCAGCTA+TGG 0.501233 5_3:-59151566 None:intergenic
TTCTTCTCTAGTGATTATGA+TGG 0.501250 5_3:+59153125 Msa0821170:CDS
GTCGGAAGATTCTCCATCTC+CGG 0.504591 5_3:+59153226 Msa0821170:CDS
CAGTGGGAGATTCTTTAGGC+TGG 0.508955 5_3:+59151793 Msa0821170:CDS
ACGGATCAACTGATGACCAT+TGG 0.511597 5_3:-59153040 None:intergenic
CGTCACTGTCTTCCTTAGGA+TGG 0.511800 5_3:-59153310 None:intergenic
TTAGGATGGTTGAAATTGGA+AGG 0.515170 5_3:-59153296 None:intergenic
CATTTCTGATAATTAACAGT+TGG 0.517247 5_3:-59151834 None:intergenic
CAGTTCCTTTACTGAAAGAA+GGG 0.524215 5_3:+59153093 Msa0821170:CDS
TTCAAAATAAATGTGACTCA+TGG 0.525741 5_3:+59153173 Msa0821170:CDS
TTCCAATTTCAACCATCCTA+AGG 0.526093 5_3:+59153298 Msa0821170:CDS
CTCCCTATTTCATTTCATGT+CGG 0.528285 5_3:-59151482 None:intergenic
TATATGTTTGAACAAGAGAG+TGG 0.543824 5_3:-59152962 None:intergenic
AGTGGATGAGAATGTAATGT+AGG 0.544501 5_3:-59151588 None:intergenic
TCACCAGAAATCGGACTAGC+CGG 0.553642 5_3:-59153245 None:intergenic
ATAATCACATTGTTTGTATG+TGG 0.560805 5_3:-59152991 None:intergenic
TGCCAAGAAAGAATTCAGCT+TGG 0.561777 5_3:+59151860 Msa0821170:CDS
GTTGACGTGAGATGTGAGCT+AGG 0.566796 5_3:+59153511 Msa0821170:three_prime_UTR
GAAATCGGACTAGCCGGAGA+TGG 0.573901 5_3:-59153239 None:intergenic
TGAATAATGGCAACTAGCAG+AGG 0.583195 5_3:-59151649 None:intergenic
GACTGGATGTATATCAGTGT+TGG 0.601352 5_3:-59153063 None:intergenic
ATAAATGTGACTCATGGACA+AGG 0.609850 5_3:+59153179 Msa0821170:CDS
TTATGATGATGCACAAGACA+AGG 0.629304 5_3:+59153010 Msa0821170:CDS
AGTTCCTTTACTGAAAGAAG+GGG 0.634152 5_3:+59153094 Msa0821170:CDS
TTGACGTGAGATGTGAGCTA+GGG 0.640215 5_3:+59153512 Msa0821170:three_prime_UTR
AGTAATTCAACAGAAAGACA+AGG 0.655740 5_3:+59151517 Msa0821170:five_prime_UTR
GGATGTATATCAGTGTTGGA+CGG 0.669469 5_3:-59153059 None:intergenic
GGAGATGGAGAATCTTCCGA+CGG 0.671736 5_3:-59153224 None:intergenic
TAAATGTGACTCATGGACAA+GGG 0.675226 5_3:+59153180 Msa0821170:CDS
AAGATGTCCCTAATAAAACA+AGG 0.680872 5_3:-59153427 None:intergenic
AAGACAAGGACACAATCCAA+TGG 0.682396 5_3:+59153024 Msa0821170:CDS
TGATATACATCCAGTCACAG+TGG 0.752267 5_3:+59153070 Msa0821170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAGTTATGCTTAAAT+TGG + chr5_3:59152460-59152479 Msa0821170:intron 15.0%
!! AAGAAAGGAATATTAAAAAA+AGG - chr5_3:59152699-59152718 None:intergenic 15.0%
!!! TTTTTAATATTCCTTTCTTT+TGG + chr5_3:59152700-59152719 Msa0821170:intron 15.0%
!! TCTTTCAAAAAAAAAAACAA+CGG - chr5_3:59153637-59153656 None:intergenic 15.0%
!! TATAAAAGTTATGCTTAAAT+TGG + chr5_3:59152460-59152479 Msa0821170:intron 15.0%
!! AAGAAAGGAATATTAAAAAA+AGG - chr5_3:59152699-59152718 None:intergenic 15.0%
!!! TTTTTAATATTCCTTTCTTT+TGG + chr5_3:59152700-59152719 Msa0821170:intron 15.0%
!! TCTTTCAAAAAAAAAAACAA+CGG - chr5_3:59153637-59153656 None:intergenic 15.0%
!! AACTGTTAATTATCAGAAAT+GGG + chr5_3:59151836-59151855 Msa0821170:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG - chr5_3:59151893-59151912 None:intergenic 20.0%
!!! ATTTTATGTTTAGACCTTTT+TGG + chr5_3:59151954-59151973 Msa0821170:intron 20.0%
!! AAAGTGGTATAAAATTAGAT+TGG + chr5_3:59152326-59152345 Msa0821170:intron 20.0%
!! AAATAAAAACATAACCACTT+AGG - chr5_3:59152406-59152425 None:intergenic 20.0%
!!! TTTTTAACAAAAGAGACAAA+TGG - chr5_3:59152619-59152638 None:intergenic 20.0%
!!! TTTGTCTCTTTTGTTAAAAA+AGG + chr5_3:59152619-59152638 Msa0821170:intron 20.0%
!! AATTAACAAACCAAACTTTA+GGG - chr5_3:59152760-59152779 None:intergenic 20.0%
!! AAATTAACAAACCAAACTTT+AGG - chr5_3:59152761-59152780 None:intergenic 20.0%
!! AAATTTCACAACATGTTATA+TGG + chr5_3:59153401-59153420 Msa0821170:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG - chr5_3:59153542-59153561 None:intergenic 20.0%
!! CTTTCAAAAAAAAAAACAAC+GGG - chr5_3:59153636-59153655 None:intergenic 20.0%
!! AACTGTTAATTATCAGAAAT+GGG + chr5_3:59151836-59151855 Msa0821170:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG - chr5_3:59151893-59151912 None:intergenic 20.0%
!!! ATTTTATGTTTAGACCTTTT+TGG + chr5_3:59151954-59151973 Msa0821170:intron 20.0%
!! AAAGTGGTATAAAATTAGAT+TGG + chr5_3:59152326-59152345 Msa0821170:intron 20.0%
!! AAATAAAAACATAACCACTT+AGG - chr5_3:59152406-59152425 None:intergenic 20.0%
!!! TTTTTAACAAAAGAGACAAA+TGG - chr5_3:59152619-59152638 None:intergenic 20.0%
!!! TTTGTCTCTTTTGTTAAAAA+AGG + chr5_3:59152619-59152638 Msa0821170:intron 20.0%
!! AATTAACAAACCAAACTTTA+GGG - chr5_3:59152760-59152779 None:intergenic 20.0%
!! AAATTAACAAACCAAACTTT+AGG - chr5_3:59152761-59152780 None:intergenic 20.0%
!! AAATTTCACAACATGTTATA+TGG + chr5_3:59153401-59153420 Msa0821170:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG - chr5_3:59153542-59153561 None:intergenic 20.0%
!! CTTTCAAAAAAAAAAACAAC+GGG - chr5_3:59153636-59153655 None:intergenic 20.0%
! TTCTATGTTCCATTATTCTT+GGG - chr5_3:59151686-59151705 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG + chr5_3:59151744-59151763 Msa0821170:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG - chr5_3:59151837-59151856 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG + chr5_3:59151835-59151854 Msa0821170:CDS 25.0%
! TCATGATATATGTTCCAAAA+AGG - chr5_3:59151971-59151990 None:intergenic 25.0%
! TTGCATTGAATTACTTCTTA+TGG + chr5_3:59152036-59152055 Msa0821170:intron 25.0%
! CTGATGATTATAATTGATTG+AGG - chr5_3:59152126-59152145 None:intergenic 25.0%
!! TATATGTGTGACTTTTATGA+TGG - chr5_3:59152178-59152197 None:intergenic 25.0%
! GTTTGTAATTGATTGTTTGA+TGG + chr5_3:59152203-59152222 Msa0821170:intron 25.0%
! TTAGAATAAAATGTCAACAC+TGG + chr5_3:59152265-59152284 Msa0821170:intron 25.0%
! TAGAATAAAATGTCAACACT+GGG + chr5_3:59152266-59152285 Msa0821170:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG - chr5_3:59152580-59152599 None:intergenic 25.0%
! GAATATTTGTACCAAAAGAA+AGG - chr5_3:59152714-59152733 None:intergenic 25.0%
! CTAGTATTAACTTCAAAATG+TGG + chr5_3:59152841-59152860 Msa0821170:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG - chr5_3:59152994-59153013 None:intergenic 25.0%
! TTCAAAATAAATGTGACTCA+TGG + chr5_3:59153173-59153192 Msa0821170:CDS 25.0%
! TGACAAAGATAAAGATAAAG+AGG + chr5_3:59153346-59153365 Msa0821170:CDS 25.0%
! TTCTATGTTCCATTATTCTT+GGG - chr5_3:59151686-59151705 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG + chr5_3:59151744-59151763 Msa0821170:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG - chr5_3:59151837-59151856 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG + chr5_3:59151835-59151854 Msa0821170:CDS 25.0%
! TCATGATATATGTTCCAAAA+AGG - chr5_3:59151971-59151990 None:intergenic 25.0%
! TTGCATTGAATTACTTCTTA+TGG + chr5_3:59152036-59152055 Msa0821170:intron 25.0%
! CTGATGATTATAATTGATTG+AGG - chr5_3:59152126-59152145 None:intergenic 25.0%
!! TATATGTGTGACTTTTATGA+TGG - chr5_3:59152178-59152197 None:intergenic 25.0%
! GTTTGTAATTGATTGTTTGA+TGG + chr5_3:59152203-59152222 Msa0821170:intron 25.0%
! TTAGAATAAAATGTCAACAC+TGG + chr5_3:59152265-59152284 Msa0821170:intron 25.0%
! TAGAATAAAATGTCAACACT+GGG + chr5_3:59152266-59152285 Msa0821170:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG - chr5_3:59152580-59152599 None:intergenic 25.0%
! GAATATTTGTACCAAAAGAA+AGG - chr5_3:59152714-59152733 None:intergenic 25.0%
! CTAGTATTAACTTCAAAATG+TGG + chr5_3:59152841-59152860 Msa0821170:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG - chr5_3:59152994-59153013 None:intergenic 25.0%
! TTCAAAATAAATGTGACTCA+TGG + chr5_3:59153173-59153192 Msa0821170:CDS 25.0%
! TGACAAAGATAAAGATAAAG+AGG + chr5_3:59153346-59153365 Msa0821170:CDS 25.0%
AAACCGACATGAAATGAAAT+AGG + chr5_3:59151479-59151498 Msa0821170:five_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG + chr5_3:59151480-59151499 Msa0821170:five_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG + chr5_3:59151517-59151536 Msa0821170:five_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+TGG - chr5_3:59151617-59151636 None:intergenic 30.0%
CTTCTATGTTCCATTATTCT+TGG - chr5_3:59151687-59151706 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG + chr5_3:59151776-59151795 Msa0821170:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG + chr5_3:59151777-59151796 Msa0821170:CDS 30.0%
AGATAGAGATCATGTACTAT+AGG - chr5_3:59152005-59152024 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG - chr5_3:59152098-59152117 None:intergenic 30.0%
TCACAAGTTGTTTATGAAAG+TGG + chr5_3:59152310-59152329 Msa0821170:intron 30.0%
!! TATGTTTTTATTTCCCCACT+TGG + chr5_3:59152413-59152432 Msa0821170:intron 30.0%
!! ATGTTTTTATTTCCCCACTT+GGG + chr5_3:59152414-59152433 Msa0821170:intron 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG + chr5_3:59152476-59152495 Msa0821170:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG + chr5_3:59152477-59152496 Msa0821170:intron 30.0%
!! ACCTATTTTGGGAATAATGT+GGG - chr5_3:59152535-59152554 None:intergenic 30.0%
TACTCAAAATGCCAAAATGA+CGG + chr5_3:59152665-59152684 Msa0821170:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG + chr5_3:59152731-59152750 Msa0821170:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG + chr5_3:59152806-59152825 Msa0821170:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG - chr5_3:59152965-59152984 None:intergenic 30.0%
TTCTTCTCTAGTGATTATGA+TGG + chr5_3:59153125-59153144 Msa0821170:CDS 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG + chr5_3:59153419-59153438 Msa0821170:CDS 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG + chr5_3:59153420-59153439 Msa0821170:CDS 30.0%
AAGATGTCCCTAATAAAACA+AGG - chr5_3:59153430-59153449 None:intergenic 30.0%
AAACCGACATGAAATGAAAT+AGG + chr5_3:59151479-59151498 Msa0821170:five_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG + chr5_3:59151480-59151499 Msa0821170:five_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG + chr5_3:59151517-59151536 Msa0821170:five_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+TGG - chr5_3:59151617-59151636 None:intergenic 30.0%
CTTCTATGTTCCATTATTCT+TGG - chr5_3:59151687-59151706 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG + chr5_3:59151776-59151795 Msa0821170:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG + chr5_3:59151777-59151796 Msa0821170:CDS 30.0%
AGATAGAGATCATGTACTAT+AGG - chr5_3:59152005-59152024 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG - chr5_3:59152098-59152117 None:intergenic 30.0%
TCACAAGTTGTTTATGAAAG+TGG + chr5_3:59152310-59152329 Msa0821170:intron 30.0%
!! TATGTTTTTATTTCCCCACT+TGG + chr5_3:59152413-59152432 Msa0821170:intron 30.0%
!! ATGTTTTTATTTCCCCACTT+GGG + chr5_3:59152414-59152433 Msa0821170:intron 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG + chr5_3:59152476-59152495 Msa0821170:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG + chr5_3:59152477-59152496 Msa0821170:intron 30.0%
!! ACCTATTTTGGGAATAATGT+GGG - chr5_3:59152535-59152554 None:intergenic 30.0%
TACTCAAAATGCCAAAATGA+CGG + chr5_3:59152665-59152684 Msa0821170:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG + chr5_3:59152731-59152750 Msa0821170:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG + chr5_3:59152806-59152825 Msa0821170:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG - chr5_3:59152965-59152984 None:intergenic 30.0%
TTCTTCTCTAGTGATTATGA+TGG + chr5_3:59153125-59153144 Msa0821170:CDS 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG + chr5_3:59153419-59153438 Msa0821170:CDS 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG + chr5_3:59153420-59153439 Msa0821170:CDS 30.0%
AAGATGTCCCTAATAAAACA+AGG - chr5_3:59153430-59153449 None:intergenic 30.0%
CTCCCTATTTCATTTCATGT+CGG - chr5_3:59151485-59151504 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG + chr5_3:59151553-59151572 Msa0821170:five_prime_UTR 35.0%
AGTGGATGAGAATGTAATGT+AGG - chr5_3:59151591-59151610 None:intergenic 35.0%
TCATGTTTCTTAGGTTCTGT+TGG + chr5_3:59151753-59151772 Msa0821170:CDS 35.0%
ACCCACATTATTCCCAAAAT+AGG + chr5_3:59152531-59152550 Msa0821170:intron 35.0%
!! GACCTATTTTGGGAATAATG+TGG - chr5_3:59152536-59152555 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG - chr5_3:59152558-59152577 None:intergenic 35.0%
! TTATGATGATGCACAAGACA+AGG + chr5_3:59153010-59153029 Msa0821170:CDS 35.0%
CAGTTCCTTTACTGAAAGAA+GGG + chr5_3:59153093-59153112 Msa0821170:CDS 35.0%
AGTTCCTTTACTGAAAGAAG+GGG + chr5_3:59153094-59153113 Msa0821170:CDS 35.0%
GATGGTGAACAATGCAAAAA+TGG + chr5_3:59153143-59153162 Msa0821170:CDS 35.0%
ATAAATGTGACTCATGGACA+AGG + chr5_3:59153179-59153198 Msa0821170:CDS 35.0%
TAAATGTGACTCATGGACAA+GGG + chr5_3:59153180-59153199 Msa0821170:CDS 35.0%
TTGGAAGGAATATTTGTGTC+TGG - chr5_3:59153284-59153303 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG - chr5_3:59153299-59153318 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG + chr5_3:59153298-59153317 Msa0821170:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG - chr5_3:59153303-59153322 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG - chr5_3:59153375-59153394 None:intergenic 35.0%
CTCCCTATTTCATTTCATGT+CGG - chr5_3:59151485-59151504 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG + chr5_3:59151553-59151572 Msa0821170:five_prime_UTR 35.0%
AGTGGATGAGAATGTAATGT+AGG - chr5_3:59151591-59151610 None:intergenic 35.0%
TCATGTTTCTTAGGTTCTGT+TGG + chr5_3:59151753-59151772 Msa0821170:CDS 35.0%
ACCCACATTATTCCCAAAAT+AGG + chr5_3:59152531-59152550 Msa0821170:intron 35.0%
!! GACCTATTTTGGGAATAATG+TGG - chr5_3:59152536-59152555 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG - chr5_3:59152558-59152577 None:intergenic 35.0%
! TTATGATGATGCACAAGACA+AGG + chr5_3:59153010-59153029 Msa0821170:CDS 35.0%
CAGTTCCTTTACTGAAAGAA+GGG + chr5_3:59153093-59153112 Msa0821170:CDS 35.0%
AGTTCCTTTACTGAAAGAAG+GGG + chr5_3:59153094-59153113 Msa0821170:CDS 35.0%
GATGGTGAACAATGCAAAAA+TGG + chr5_3:59153143-59153162 Msa0821170:CDS 35.0%
ATAAATGTGACTCATGGACA+AGG + chr5_3:59153179-59153198 Msa0821170:CDS 35.0%
TAAATGTGACTCATGGACAA+GGG + chr5_3:59153180-59153199 Msa0821170:CDS 35.0%
TTGGAAGGAATATTTGTGTC+TGG - chr5_3:59153284-59153303 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG - chr5_3:59153299-59153318 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG + chr5_3:59153298-59153317 Msa0821170:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG - chr5_3:59153303-59153322 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG - chr5_3:59153375-59153394 None:intergenic 35.0%
TGAATAATGGCAACTAGCAG+AGG - chr5_3:59151652-59151671 None:intergenic 40.0%
GGTGAGCGAAATGTGAATAA+TGG - chr5_3:59151665-59151684 None:intergenic 40.0%
TTTCGCTCACCCAAGAATAA+TGG + chr5_3:59151674-59151693 Msa0821170:exon 40.0%
TACACAGTGGGAGATTCTTT+AGG + chr5_3:59151789-59151808 Msa0821170:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG + chr5_3:59151860-59151879 Msa0821170:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG + chr5_3:59151861-59151880 Msa0821170:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG - chr5_3:59151865-59151884 None:intergenic 40.0%
AATGTCAACACTGGGAATAG+TGG + chr5_3:59152274-59152293 Msa0821170:intron 40.0%
ACTATAGTGTCACACCTAAG+TGG + chr5_3:59152389-59152408 Msa0821170:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG + chr5_3:59152497-59152516 Msa0821170:intron 40.0%
!!! CTTGAGTTGGGACCTATTTT+GGG - chr5_3:59152546-59152565 None:intergenic 40.0%
!!! ACTTGAGTTGGGACCTATTT+TGG - chr5_3:59152547-59152566 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG - chr5_3:59152559-59152578 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG + chr5_3:59153024-59153043 Msa0821170:CDS 40.0%
! GGATGTATATCAGTGTTGGA+CGG - chr5_3:59153062-59153081 None:intergenic 40.0%
GACTGGATGTATATCAGTGT+TGG - chr5_3:59153066-59153085 None:intergenic 40.0%
TGATATACATCCAGTCACAG+TGG + chr5_3:59153070-59153089 Msa0821170:CDS 40.0%
GCAGTTCCTTTACTGAAAGA+AGG + chr5_3:59153092-59153111 Msa0821170:CDS 40.0%
!! GACGGTTTTTGAAGACTCTT+CGG - chr5_3:59153209-59153228 None:intergenic 40.0%
GAAGAGTCTTCAAAAACCGT+CGG + chr5_3:59153208-59153227 Msa0821170:CDS 40.0%
TGAATAATGGCAACTAGCAG+AGG - chr5_3:59151652-59151671 None:intergenic 40.0%
GGTGAGCGAAATGTGAATAA+TGG - chr5_3:59151665-59151684 None:intergenic 40.0%
TTTCGCTCACCCAAGAATAA+TGG + chr5_3:59151674-59151693 Msa0821170:exon 40.0%
TACACAGTGGGAGATTCTTT+AGG + chr5_3:59151789-59151808 Msa0821170:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG + chr5_3:59151860-59151879 Msa0821170:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG + chr5_3:59151861-59151880 Msa0821170:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG - chr5_3:59151865-59151884 None:intergenic 40.0%
AATGTCAACACTGGGAATAG+TGG + chr5_3:59152274-59152293 Msa0821170:intron 40.0%
ACTATAGTGTCACACCTAAG+TGG + chr5_3:59152389-59152408 Msa0821170:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG + chr5_3:59152497-59152516 Msa0821170:intron 40.0%
!!! CTTGAGTTGGGACCTATTTT+GGG - chr5_3:59152546-59152565 None:intergenic 40.0%
!!! ACTTGAGTTGGGACCTATTT+TGG - chr5_3:59152547-59152566 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG - chr5_3:59152559-59152578 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG + chr5_3:59153024-59153043 Msa0821170:CDS 40.0%
! GGATGTATATCAGTGTTGGA+CGG - chr5_3:59153062-59153081 None:intergenic 40.0%
GACTGGATGTATATCAGTGT+TGG - chr5_3:59153066-59153085 None:intergenic 40.0%
TGATATACATCCAGTCACAG+TGG + chr5_3:59153070-59153089 Msa0821170:CDS 40.0%
GCAGTTCCTTTACTGAAAGA+AGG + chr5_3:59153092-59153111 Msa0821170:CDS 40.0%
!! GACGGTTTTTGAAGACTCTT+CGG - chr5_3:59153209-59153228 None:intergenic 40.0%
GAAGAGTCTTCAAAAACCGT+CGG + chr5_3:59153208-59153227 Msa0821170:CDS 40.0%
GATTCATATGCTGCCAGCTA+TGG - chr5_3:59151569-59151588 None:intergenic 45.0%
!! AGCTTTTATGACTGGCTCAG+TGG - chr5_3:59151609-59151628 None:intergenic 45.0%
AATGCCAAAATGACGGTTGC+TGG + chr5_3:59152672-59152691 Msa0821170:intron 45.0%
TGAGTGGCTACCCTAAAGTT+TGG + chr5_3:59152747-59152766 Msa0821170:intron 45.0%
ACGGATCAACTGATGACCAT+TGG - chr5_3:59153043-59153062 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG - chr5_3:59153101-59153120 None:intergenic 45.0%
GACTCATCGTCACCAGAAAT+CGG - chr5_3:59153257-59153276 None:intergenic 45.0%
TCATCGTCACTGTCTTCCTT+AGG - chr5_3:59153317-59153336 None:intergenic 45.0%
TTGACGTGAGATGTGAGCTA+GGG + chr5_3:59153512-59153531 Msa0821170:three_prime_UTR 45.0%
GATTCATATGCTGCCAGCTA+TGG - chr5_3:59151569-59151588 None:intergenic 45.0%
!! AGCTTTTATGACTGGCTCAG+TGG - chr5_3:59151609-59151628 None:intergenic 45.0%
AATGCCAAAATGACGGTTGC+TGG + chr5_3:59152672-59152691 Msa0821170:intron 45.0%
TGAGTGGCTACCCTAAAGTT+TGG + chr5_3:59152747-59152766 Msa0821170:intron 45.0%
ACGGATCAACTGATGACCAT+TGG - chr5_3:59153043-59153062 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG - chr5_3:59153101-59153120 None:intergenic 45.0%
GACTCATCGTCACCAGAAAT+CGG - chr5_3:59153257-59153276 None:intergenic 45.0%
TCATCGTCACTGTCTTCCTT+AGG - chr5_3:59153317-59153336 None:intergenic 45.0%
TTGACGTGAGATGTGAGCTA+GGG + chr5_3:59153512-59153531 Msa0821170:three_prime_UTR 45.0%
CAGTGGGAGATTCTTTAGGC+TGG + chr5_3:59151793-59151812 Msa0821170:CDS 50.0%
ATGCCACACATAGCCCAAGT+GGG - chr5_3:59152430-59152449 None:intergenic 50.0%
AATGCCACACATAGCCCAAG+TGG - chr5_3:59152431-59152450 None:intergenic 50.0%
! AGGTCCAGCAACCGTCATTT+TGG - chr5_3:59152679-59152698 None:intergenic 50.0%
AAAGGAACTGCCACTGTGAC+TGG - chr5_3:59153083-59153102 None:intergenic 50.0%
GGAGATGGAGAATCTTCCGA+CGG - chr5_3:59153227-59153246 None:intergenic 50.0%
GTCGGAAGATTCTCCATCTC+CGG + chr5_3:59153226-59153245 Msa0821170:CDS 50.0%
!! TCACCAGAAATCGGACTAGC+CGG - chr5_3:59153248-59153267 None:intergenic 50.0%
CGTCACTGTCTTCCTTAGGA+TGG - chr5_3:59153313-59153332 None:intergenic 50.0%
GTTGACGTGAGATGTGAGCT+AGG + chr5_3:59153511-59153530 Msa0821170:three_prime_UTR 50.0%
CAGTGGGAGATTCTTTAGGC+TGG + chr5_3:59151793-59151812 Msa0821170:CDS 50.0%
ATGCCACACATAGCCCAAGT+GGG - chr5_3:59152430-59152449 None:intergenic 50.0%
AATGCCACACATAGCCCAAG+TGG - chr5_3:59152431-59152450 None:intergenic 50.0%
! AGGTCCAGCAACCGTCATTT+TGG - chr5_3:59152679-59152698 None:intergenic 50.0%
AAAGGAACTGCCACTGTGAC+TGG - chr5_3:59153083-59153102 None:intergenic 50.0%
GGAGATGGAGAATCTTCCGA+CGG - chr5_3:59153227-59153246 None:intergenic 50.0%
GTCGGAAGATTCTCCATCTC+CGG + chr5_3:59153226-59153245 Msa0821170:CDS 50.0%
!! TCACCAGAAATCGGACTAGC+CGG - chr5_3:59153248-59153267 None:intergenic 50.0%
CGTCACTGTCTTCCTTAGGA+TGG - chr5_3:59153313-59153332 None:intergenic 50.0%
GTTGACGTGAGATGTGAGCT+AGG + chr5_3:59153511-59153530 Msa0821170:three_prime_UTR 50.0%
TTCCCCACTTGGGCTATGTG+TGG + chr5_3:59152424-59152443 Msa0821170:intron 55.0%
TGCCACACATAGCCCAAGTG+GGG - chr5_3:59152429-59152448 None:intergenic 55.0%
!! GAAATCGGACTAGCCGGAGA+TGG - chr5_3:59153242-59153261 None:intergenic 55.0%
! TCTCCGGCTAGTCCGATTTC+TGG + chr5_3:59153242-59153261 Msa0821170:CDS 55.0%
TTCCCCACTTGGGCTATGTG+TGG + chr5_3:59152424-59152443 Msa0821170:intron 55.0%
TGCCACACATAGCCCAAGTG+GGG - chr5_3:59152429-59152448 None:intergenic 55.0%
!! GAAATCGGACTAGCCGGAGA+TGG - chr5_3:59153242-59153261 None:intergenic 55.0%
! TCTCCGGCTAGTCCGATTTC+TGG + chr5_3:59153242-59153261 Msa0821170:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 59151476 59153667 59151476 ID=Msa0821170;Name=Msa0821170
chr5_3 mRNA 59151476 59153667 59151476 ID=Msa0821170-mRNA-1;Parent=Msa0821170;Name=Msa0821170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=217|1|1|1|1|1|2|207|239
chr5_3 exon 59151476 59151894 59151476 ID=Msa0821170-mRNA-1:exon:17933;Parent=Msa0821170-mRNA-1
chr5_3 exon 59152943 59153667 59152943 ID=Msa0821170-mRNA-1:exon:17934;Parent=Msa0821170-mRNA-1
chr5_3 five_prime_UTR 59151476 59151692 59151476 ID=Msa0821170-mRNA-1:five_prime_utr;Parent=Msa0821170-mRNA-1
chr5_3 CDS 59151693 59151894 59151693 ID=Msa0821170-mRNA-1:cds;Parent=Msa0821170-mRNA-1
chr5_3 CDS 59152943 59153460 59152943 ID=Msa0821170-mRNA-1:cds;Parent=Msa0821170-mRNA-1
chr5_3 three_prime_UTR 59153461 59153667 59153461 ID=Msa0821170-mRNA-1:three_prime_utr;Parent=Msa0821170-mRNA-1
Gene Sequence

>Msa0821170

ATGGAACATAGAAGAAACTCATCATTGTTTCTATTCTCAACTCTTTCTTGTCTTTTTCTATCATGTTTCTTAGGTTCTGTTGGAGCTTACAAAAATTACACAGTGGGAGATTCTTTAGGCTGGTTTGACAATCTTGAAAAGCCAACTGTTAATTATCAGAAATGGGTTGCCAAGAAAGAATTCAGCTTGGGAGATTTCCTCATTTTCAACACAGACAATAACCACTCTCTTGTTCAAACATATAATTTCACCACATACAAACAATGTGATTATGATGATGCACAAGACAAGGACACAATCCAATGGTCATCAGTTGATCCGTCCAACACTGATATACATCCAGTCACAGTGGCAGTTCCTTTACTGAAAGAAGGGGCAACATATTTCTTCTCTAGTGATTATGATGGTGAACAATGCAAAAATGGACAACATTTCAAAATAAATGTGACTCATGGACAAGGGTTGCCGAAGAGTCTTCAAAAACCGTCGGAAGATTCTCCATCTCCGGCTAGTCCGATTTCTGGTGACGATGAGTCTGCACCAGACACAAATATTCCTTCCAATTTCAACCATCCTAAGGAAGACAGTGACGATGAAAAAACTAGTGACAAAGATAAAGATAAAGAGGAATCCAGTTCTGTTTCGATGTTAAAGTATGCAAAATTTCACAACATGTTATATGGATGCCTTGTTTTATTAGGGACATCTTTCTTTTTTTAG
Protein sequence

>Msa0821170

MEHRRNSSLFLFSTLSCLFLSCFLGSVGAYKNYTVGDSLGWFDNLEKPTVNYQKWVAKKEFSLGDFLIFNTDNNHSLVQTYNFTTYKQCDYDDAQDKDTIQWSSVDPSNTDIHPVTVAVPLLKEGATYFFSSDYDGEQCKNGQHFKINVTHGQGLPKSLQKPSEDSPSPASPISGDDESAPDTNIPSNFNHPKEDSDDEKTSDKDKDKEESSSVSMLKYAKFHNMLYGCLVLLGTSFFF*