AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0032570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032570 MtrunA17_Chr1g0192471 97.400 423 11 0 1 423 1 423 0.0 846
Msa0032570 MtrunA17_Chr7g0271971 85.071 422 61 2 1 422 1 420 0.0 717
Msa0032570 MtrunA17_Chr1g0209451 68.396 424 130 3 1 422 1 422 0.0 588
Msa0032570 MtrunA17_Chr7g0225501 62.673 434 137 13 1 422 19 439 1.15e-167 478
Msa0032570 MtrunA17_Chr1g0153671 56.098 410 178 2 14 423 11 418 3.65e-167 476
Msa0032570 MtrunA17_Chr3g0133801 55.556 414 175 3 14 422 10 419 4.04e-163 466
Msa0032570 MtrunA17_Chr2g0326251 64.352 216 58 9 109 317 7 210 1.15e-72 227
Msa0032570 MtrunA17_Chr1g0150451 31.449 283 155 12 25 298 72 324 1.65e-28 114
Msa0032570 MtrunA17_Chr3g0136711 30.924 249 144 9 57 298 54 281 2.97e-27 110
Msa0032570 MtrunA17_Chr1g0212961 31.673 281 155 14 25 299 90 339 1.32e-25 106
Msa0032570 MtrunA17_Chr3g0105351 29.779 272 163 11 33 297 64 314 3.95e-25 104
Msa0032570 MtrunA17_Chr3g0105371 30.037 273 161 10 33 297 88 338 6.07e-25 104
Msa0032570 MtrunA17_Chr5g0430481 28.625 269 164 11 36 297 127 374 1.87e-22 98.2
Msa0032570 MtrunA17_Chr8g0360351 30.043 233 139 8 68 295 66 279 2.75e-21 93.2
Msa0032570 MtrunA17_Chr2g0317021 29.572 257 152 11 46 297 100 332 3.17e-20 91.3
Msa0032570 MtrunA17_Chr4g0070871 27.365 296 159 10 46 297 220 503 5.92e-20 92.0
Msa0032570 MtrunA17_Chr3g0126511 29.020 255 152 9 46 295 108 338 2.21e-19 89.0
Msa0032570 MtrunA17_Chr4g0050381 27.698 278 168 10 27 295 21 274 4.33e-19 87.0
Msa0032570 MtrunA17_Chr1g0171571 31.301 246 136 7 69 299 419 646 4.98e-19 89.7
Msa0032570 MtrunA17_Chr5g0407161 26.894 264 153 9 67 314 123 362 1.56e-18 86.7
Msa0032570 MtrunA17_Chr3g0115851 29.032 279 163 11 27 295 26 279 2.88e-18 84.7
Msa0032570 MtrunA17_Chr2g0279151 25.818 275 177 9 27 295 25 278 3.68e-18 84.3
Msa0032570 MtrunA17_Chr5g0426141 29.137 278 164 12 27 295 26 279 9.43e-18 83.2
Msa0032570 MtrunA17_Chr5g0396731 29.524 210 123 7 66 265 105 299 1.97e-17 83.2
Msa0032570 MtrunA17_Chr1g0150781 30.712 267 137 10 69 299 291 545 1.01e-16 82.4
Msa0032570 MtrunA17_Chr4g0051661 29.921 254 150 10 46 295 166 395 1.09e-16 81.3
Msa0032570 MtrunA17_Chr3g0112291 29.032 217 122 6 62 261 117 318 1.70e-16 80.9
Msa0032570 MtrunA17_Chr2g0299981 25.857 321 162 10 40 297 228 535 2.16e-16 81.3
Msa0032570 MtrunA17_Chr6g0466641 87.805 41 5 0 1 41 1 41 2.54e-16 73.9
Msa0032570 MtrunA17_Chr8g0389921 29.442 197 118 6 69 258 199 381 3.31e-16 80.5
Msa0032570 MtrunA17_Chr4g0065141 29.762 252 132 10 65 295 121 348 4.16e-16 79.7
Msa0032570 MtrunA17_Chr2g0293811 31.373 255 131 11 64 295 96 329 5.53e-16 79.0
Msa0032570 MtrunA17_Chr6g0483411 28.053 303 165 13 26 295 34 316 6.47e-16 78.2
Msa0032570 MtrunA17_Chr3g0135961 27.574 272 149 8 62 297 280 539 1.03e-15 79.3
Msa0032570 MtrunA17_Chr1g0194191 28.464 267 155 11 67 314 117 366 1.79e-15 77.8
Msa0032570 MtrunA17_Chr7g0272061 33.898 177 95 9 116 276 165 335 1.06e-14 75.1
Msa0032570 MtrunA17_Chr1g0158781 31.048 248 138 9 69 297 146 379 1.10e-14 75.1
Msa0032570 MtrunA17_Chr5g0435721 28.019 207 123 7 69 262 224 417 4.69e-14 73.9
Msa0032570 MtrunA17_Chr7g0221691 30.403 273 142 14 36 283 139 388 9.87e-14 72.8
Msa0032570 MtrunA17_Chr6g0486661 27.517 298 167 14 36 297 97 381 1.76e-13 72.8
Msa0032570 MtrunA17_Chr3g0138281 27.969 261 145 9 67 308 120 356 2.03e-13 71.2
Msa0032570 MtrunA17_Chr4g0066371 31.126 302 148 19 36 297 59 340 4.93e-13 70.5
Msa0032570 MtrunA17_Chr5g0394181 27.907 258 140 11 66 297 360 597 1.15e-12 69.7
Msa0032570 MtrunA17_Chr4g0001761 34.043 141 66 5 128 257 156 280 3.69e-12 67.4
Msa0032570 MtrunA17_Chr2g0286951 27.431 288 163 12 43 297 67 341 4.33e-12 67.4
Msa0032570 MtrunA17_Chr8g0342431 30.189 159 94 7 136 283 201 353 3.39e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032570 AT1G09160.2 75.355 422 102 1 1 422 1 420 0.0 647
Msa0032570 AT1G09160.1 75.355 422 102 1 1 422 1 420 0.0 647
Msa0032570 AT1G68410.4 68.434 415 127 2 11 422 16 429 0.0 577
Msa0032570 AT1G68410.3 68.434 415 127 2 11 422 16 429 0.0 577
Msa0032570 AT1G68410.1 68.434 415 127 2 11 422 16 429 0.0 577
Msa0032570 AT1G68410.2 68.434 415 127 2 11 422 16 429 0.0 577
Msa0032570 AT1G47380.1 56.341 410 178 1 14 423 10 418 5.14e-170 483
Msa0032570 AT5G10740.1 30.921 304 168 13 6 298 8 280 4.31e-27 111
Msa0032570 AT5G10740.2 30.921 304 168 13 6 298 8 280 4.31e-27 111
Msa0032570 AT2G34740.1 33.054 239 136 8 65 298 116 335 8.02e-27 110
Msa0032570 AT1G07160.1 30.686 277 157 11 33 297 123 376 1.29e-25 107
Msa0032570 AT1G67820.2 32.787 244 139 10 34 270 78 303 1.46e-25 108
Msa0032570 AT5G24940.1 30.877 285 154 13 25 298 28 280 1.79e-25 108
Msa0032570 AT1G67820.1 32.787 244 139 10 34 270 114 339 1.91e-25 108
Msa0032570 AT4G31750.2 30.108 279 158 11 25 295 28 277 3.27e-25 105
Msa0032570 AT4G31750.1 30.357 280 156 12 25 295 28 277 3.93e-25 105
Msa0032570 AT4G31750.3 30.357 280 156 12 25 295 28 277 3.93e-25 105
Msa0032570 AT1G43900.1 31.970 269 148 14 36 295 124 366 3.66e-24 103
Msa0032570 AT1G22280.3 30.901 233 143 7 68 295 65 284 4.79e-24 101
Msa0032570 AT2G40180.1 28.269 283 183 8 21 297 117 385 9.86e-24 102
Msa0032570 AT1G34750.2 29.749 279 161 11 27 295 26 279 1.59e-23 100
Msa0032570 AT1G34750.1 29.749 279 161 11 27 295 26 279 1.59e-23 100
Msa0032570 AT1G34750.3 29.749 279 161 11 27 295 26 279 1.59e-23 100
Msa0032570 AT1G78200.1 30.357 280 161 11 27 295 24 280 2.00e-23 99.8
Msa0032570 AT1G78200.3 30.357 280 161 11 27 295 24 280 2.00e-23 99.8
Msa0032570 AT1G78200.2 30.357 280 161 11 27 295 24 280 2.00e-23 99.8
Msa0032570 AT1G78200.5 31.128 257 147 10 46 295 2 235 2.22e-23 98.6
Msa0032570 AT1G78200.4 31.128 257 147 10 46 295 2 235 2.22e-23 98.6
Msa0032570 AT4G28400.3 29.258 229 146 6 68 295 45 258 8.46e-23 97.4
Msa0032570 AT2G30020.1 28.782 271 163 11 36 297 143 392 1.08e-22 99.8
Msa0032570 AT5G53140.1 29.412 255 151 10 46 295 115 345 1.16e-22 99.8
Msa0032570 AT5G53140.2 29.412 255 151 10 46 295 115 345 1.16e-22 99.8
Msa0032570 AT4G28400.1 29.258 229 146 6 68 295 67 280 1.46e-22 97.4
Msa0032570 AT1G22280.1 30.901 233 137 8 68 295 65 278 3.43e-22 96.3
Msa0032570 AT2G20630.1 29.104 268 158 12 33 295 36 276 5.57e-22 95.5
Msa0032570 AT4G26080.1 27.737 274 149 7 61 299 165 424 1.35e-21 96.7
Msa0032570 AT3G51470.2 28.475 295 169 11 36 314 32 300 2.03e-21 94.7
Msa0032570 AT2G20630.2 28.839 267 158 12 33 294 36 275 2.19e-21 94.0
Msa0032570 AT3G51470.1 28.475 295 169 11 36 314 76 344 2.35e-21 95.1
Msa0032570 AT5G57050.2 28.464 267 145 9 65 297 117 371 3.91e-20 92.0
Msa0032570 AT5G57050.1 28.464 267 145 9 65 297 157 411 5.02e-20 92.0
Msa0032570 AT2G40860.1 29.286 280 157 9 36 299 396 650 1.48e-19 91.7
Msa0032570 AT2G25620.1 30.451 266 136 10 67 308 127 367 1.49e-19 90.5
Msa0032570 AT2G25620.2 30.451 266 136 10 67 308 127 367 1.49e-19 90.5
Msa0032570 AT2G40860.4 29.286 280 157 9 36 299 298 552 2.49e-19 90.9
Msa0032570 AT2G34740.3 29.665 209 127 5 65 270 112 303 2.70e-19 88.6
Msa0032570 AT2G34740.2 29.665 209 127 5 65 270 130 321 3.20e-19 88.6
Msa0032570 AT3G62260.3 29.151 271 157 12 67 319 26 279 1.15e-17 83.6
Msa0032570 AT2G40860.2 28.409 264 148 8 36 282 396 635 1.22e-17 85.9
Msa0032570 AT1G48040.1 32.692 260 129 12 63 295 107 347 2.10e-17 84.0
Msa0032570 AT3G62260.2 29.151 271 157 12 67 319 116 369 4.81e-17 82.8
Msa0032570 AT3G62260.1 29.151 271 157 12 67 319 115 368 5.10e-17 82.8
Msa0032570 AT3G15260.2 29.185 233 141 8 68 295 73 286 5.39e-17 81.3
Msa0032570 AT3G15260.1 29.185 233 141 8 68 295 73 286 5.39e-17 81.3
Msa0032570 AT1G34750.4 28.085 235 136 9 27 252 26 236 1.27e-16 79.3
Msa0032570 AT5G59220.1 28.287 251 150 10 31 265 107 343 2.95e-15 77.8
Msa0032570 AT4G28400.2 27.568 185 120 4 68 252 67 237 4.43e-15 75.1
Msa0032570 AT1G18030.1 29.020 255 135 10 69 295 110 346 5.45e-15 76.3
Msa0032570 AT1G07430.1 28.054 221 126 7 62 265 151 355 4.95e-14 73.9
Msa0032570 AT4G27800.1 24.848 330 173 12 40 322 67 368 1.38e-13 72.4
Msa0032570 AT3G11410.1 25.446 224 126 7 62 260 131 338 2.30e-12 68.6
Msa0032570 AT2G40860.3 29.114 237 127 8 36 255 396 608 3.22e-12 68.9
Msa0032570 AT1G16220.1 23.864 352 195 13 5 301 45 378 3.94e-12 68.2
Msa0032570 AT3G17250.1 28.063 253 140 13 67 295 157 391 4.20e-12 68.2
Msa0032570 AT4G27800.3 26.119 268 134 9 40 265 67 312 4.33e-12 67.4
Msa0032570 AT4G27800.2 26.119 268 134 9 40 265 67 312 4.36e-12 67.4
Msa0032570 AT1G79630.2 29.798 198 121 6 131 314 151 344 7.94e-12 67.0
Msa0032570 AT1G79630.7 29.798 198 121 6 131 314 151 344 7.94e-12 67.0
Msa0032570 AT5G27930.3 33.544 158 88 7 105 251 150 301 9.05e-12 66.2
Msa0032570 AT1G79630.6 29.500 200 123 6 131 316 111 306 1.02e-11 66.6
Msa0032570 AT1G79630.3 29.500 200 123 6 131 316 111 306 1.02e-11 66.6
Msa0032570 AT1G79630.4 29.500 200 123 6 131 316 111 306 1.02e-11 66.6
Msa0032570 AT1G79630.5 29.500 200 123 6 131 316 111 306 1.02e-11 66.6
Msa0032570 AT1G79630.8 29.500 200 123 6 131 316 217 412 1.06e-11 67.0
Msa0032570 AT1G17550.1 27.434 226 120 8 69 265 238 448 1.14e-11 67.0
Msa0032570 AT1G17550.2 27.434 226 120 8 69 265 238 448 1.14e-11 67.0
Msa0032570 AT1G79630.1 29.500 200 123 6 131 316 221 416 1.39e-11 66.6
Msa0032570 AT4G32950.1 30.114 176 110 5 131 297 138 309 4.12e-11 64.3
Msa0032570 AT5G51760.2 25.301 249 152 6 40 265 86 323 4.23e-11 64.7
Msa0032570 AT2G29380.1 27.928 222 132 8 59 265 103 311 4.52e-11 64.3
Msa0032570 AT1G18030.2 30.288 208 104 9 69 253 110 299 4.84e-11 63.9
Msa0032570 AT5G51760.1 25.301 249 152 6 40 265 116 353 5.23e-11 64.7
Msa0032570 AT5G36250.1 26.222 225 136 9 100 300 171 389 5.36e-11 64.7
Msa0032570 AT1G72770.4 25.090 279 143 11 69 297 239 501 5.51e-11 64.7
Msa0032570 AT1G72770.1 25.090 279 143 11 69 297 239 501 5.51e-11 64.7
Msa0032570 AT1G72770.3 25.090 279 143 11 69 297 239 501 5.51e-11 64.7
Msa0032570 AT2G25070.1 31.206 141 70 5 128 257 156 280 8.92e-11 63.5
Msa0032570 AT2G25070.2 31.206 141 70 5 128 257 156 280 8.92e-11 63.5

Find 137 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCTTCTGATGGAATT+TGG 0.123861 1_1:+59750846 Msa0032570:CDS
AGCACATTATGTTTCTTTCT+TGG 0.197447 1_1:-59751140 None:intergenic
GTTCCTTCCAATACAAATTC+TGG 0.255485 1_1:+59751775 Msa0032570:CDS
TGTTTCTTTCTTGGAGTTGC+TGG 0.268275 1_1:-59751131 None:intergenic
CTGTGCTCTCAACACCCATT+AGG 0.273813 1_1:+59748952 Msa0032570:five_prime_UTR
GTGCTTAGTTCACTTCTCTT+TGG 0.288508 1_1:+59751158 Msa0032570:CDS
TTACCTGCAGGGGAAACTTC+TGG 0.307065 1_1:+59750303 Msa0032570:intron
GCAATGCTTGGTTCTACATT+TGG 0.316695 1_1:+59752046 Msa0032570:three_prime_UTR
TATAGCTTCTGATGGAATTT+GGG 0.324404 1_1:+59750847 Msa0032570:CDS
ACTGTTGTTGCTCGGACTTC+AGG 0.329448 1_1:-59751956 None:intergenic
GCTACGTTTGTGATTATTGA+TGG 0.331297 1_1:+59750333 Msa0032570:CDS
TGTGCTCTCAACACCCATTA+GGG 0.338960 1_1:+59748953 Msa0032570:five_prime_UTR
CCGTTACAGTTGCATCTGTT+GGG 0.351128 1_1:+59750361 Msa0032570:CDS
CAATGCTTGGTTCTACATTT+GGG 0.352020 1_1:+59752047 Msa0032570:three_prime_UTR
ACAGATTGCCATAGAGTTCC+TGG 0.368715 1_1:+59749683 Msa0032570:CDS
ACTTGCTTAACATGTGGTAT+TGG 0.372029 1_1:-59750690 None:intergenic
TGCTGCCAAGCTGGTGGTTA+AGG 0.391611 1_1:+59750925 Msa0032570:CDS
TAATTTGCAACAACCTTCAA+TGG 0.406917 1_1:-59752133 None:intergenic
AAGACGCCATGGAAGGAAAA+AGG 0.416517 1_1:+59751932 Msa0032570:CDS
CAGATCCTTGACGGGCATAA+TGG 0.417208 1_1:+59750055 Msa0032570:intron
GGTTGAGAATTGAGTTAGCT+AGG 0.417286 1_1:+59752104 Msa0032570:three_prime_UTR
AACTTGAACAGGAAGCTCTA+AGG 0.419981 1_1:+59751033 Msa0032570:intron
GGGCCTCTTCGCTGTTGGCC+TGG 0.420476 1_1:+59750612 Msa0032570:CDS
GTAGGAAGACTCAACGTATT+CGG 0.426631 1_1:+59750498 Msa0032570:CDS
GTTTCCTAGCTTTCAAATGC+TGG 0.427993 1_1:+59750812 Msa0032570:intron
CTTGATTGGCAGATCCTTGA+CGG 0.435403 1_1:+59750046 Msa0032570:intron
TGGAAGAAAATGTAGAAGAG+AGG 0.437691 1_1:+59750448 Msa0032570:CDS
GTGAATACCAGAATTTGTAT+TGG 0.439513 1_1:-59751782 None:intergenic
CTTAAAATGCAGGCTAGGTA+AGG 0.443855 1_1:+59751750 Msa0032570:intron
ATGGCTTCAAGCACTGCCTC+GGG 0.448779 1_1:+59750159 Msa0032570:CDS
ACTAATAAGCTTTCTGCAGT+TGG 0.455037 1_1:+59751212 Msa0032570:CDS
TCGTAGCTCACGGCCGATCA+AGG 0.456909 1_1:-59749579 None:intergenic
GTTGCTGGCATCTGCAACAC+AGG 0.461352 1_1:-59751116 None:intergenic
GGATACTTTGTCTTCTGATA+CGG 0.461894 1_1:+59750868 Msa0032570:CDS
AATGGCTTCAAGCACTGCCT+CGG 0.462446 1_1:+59750158 Msa0032570:CDS
ATCGGCCGTGAGCTACGAAA+TGG 0.463670 1_1:+59749584 Msa0032570:CDS
GTTCCGTTGGCAACCTTGAT+CGG 0.465033 1_1:+59749566 Msa0032570:CDS
CCATACCGCAAGGTGTAAGC+AGG 0.465666 1_1:+59750131 Msa0032570:CDS
AGAATTGAGTTAGCTAGGTT+AGG 0.469351 1_1:+59752109 Msa0032570:three_prime_UTR
TTTGGCTTGTGCTCAGCTGT+TGG 0.469383 1_1:+59752159 Msa0032570:three_prime_UTR
AAGAATCGCCAGGAACTCTA+TGG 0.471757 1_1:-59749691 None:intergenic
GCCTGTCCATACTTCACAAA+AGG 0.476504 1_1:-59749620 None:intergenic
GAAGTATGGACAGGCTGGTT+TGG 0.479502 1_1:+59749628 Msa0032570:CDS
GTGGAGGAGTTATTTGAAGA+GGG 0.481037 1_1:+59751239 Msa0032570:CDS
CTGGGTATCTAATATGCATC+GGG 0.481436 1_1:-59750388 None:intergenic
ACCGTTACAGTTGCATCTGT+TGG 0.483048 1_1:+59750360 Msa0032570:CDS
TGCAGAACTTGCTGCCAAGC+TGG 0.486136 1_1:+59750916 Msa0032570:CDS
CCTGCTTACACCTTGCGGTA+TGG 0.486759 1_1:-59750131 None:intergenic
TCTTCCTACTTCACCACCAC+TGG 0.489968 1_1:-59750484 None:intergenic
AATAATATCTACAACAAGGC+AGG 0.494608 1_1:-59751081 None:intergenic
GCTTTCTGCAGTTGGTATTG+TGG 0.496447 1_1:+59751220 Msa0032570:CDS
AGCTAGGTTAGGTCCATTGA+AGG 0.499451 1_1:+59752120 Msa0032570:three_prime_UTR
TGACAGTTTGTGCAGAGGAA+TGG 0.499633 1_1:-59751901 None:intergenic
CCTTCCCATGGCTTAGATAC+CGG 0.499863 1_1:-59751877 None:intergenic
TGCCTAGCAGATCAACCATC+TGG 0.501426 1_1:+59751817 Msa0032570:CDS
TTTGTGAAGTATGGACAGGC+TGG 0.503204 1_1:+59749623 Msa0032570:CDS
AAAATGTAGAAGAGAGGGAG+CGG 0.505425 1_1:+59750454 Msa0032570:CDS
TATATTGATTTGATTGCTAA+CGG 0.510016 1_1:+59752298 Msa0032570:three_prime_UTR
CGTTTGTGATTATTGATGGA+TGG 0.510062 1_1:+59750337 Msa0032570:CDS
AGAGAGAAACAACACCTCCC+TGG 0.510635 1_1:-59750407 None:intergenic
TTGGCAGTGTGAGCAATGCT+TGG 0.513571 1_1:+59752034 Msa0032570:three_prime_UTR
TTCTGCAGTTGGTATTGTGG+AGG 0.515246 1_1:+59751223 Msa0032570:CDS
AGCTTGGCAGCAAGTTCTGC+AGG 0.516289 1_1:-59750914 None:intergenic
AGGTAGGGCCTCTTCGCTGT+TGG 0.519693 1_1:+59750607 Msa0032570:intron
ACAGCCAGTGGTGGTGAAGT+AGG 0.520024 1_1:+59750480 Msa0032570:CDS
CGTTACAGTTGCATCTGTTG+GGG 0.531574 1_1:+59750362 Msa0032570:CDS
TTGATTGGCAGATCCTTGAC+GGG 0.533037 1_1:+59750047 Msa0032570:intron
GAAGCAACCACTTGTTCCGT+TGG 0.533409 1_1:+59749553 Msa0032570:CDS
TGAGTAAGACTGAATTATCA+AGG 0.534598 1_1:+59749528 Msa0032570:CDS
ATAAAATGATCGGTGTTCAC+TGG 0.535304 1_1:-59751850 None:intergenic
CCTCTTCGCTGTTGGCCTGG+TGG 0.536837 1_1:+59750615 Msa0032570:CDS
AAATGTAGAAGAGAGGGAGC+GGG 0.539423 1_1:+59750455 Msa0032570:CDS
TGTGGAGGAGTTATTTGAAG+AGG 0.540145 1_1:+59751238 Msa0032570:CDS
AATGACGTTGAAGAATCGCC+AGG 0.544088 1_1:-59749701 None:intergenic
CCTGGGTATCTAATATGCAT+CGG 0.544206 1_1:-59750389 None:intergenic
ATACCAGAATTTGTATTGGA+AGG 0.544653 1_1:-59751778 None:intergenic
TCCTCCCTGCTTACACCTTG+CGG 0.545928 1_1:-59750136 None:intergenic
TCACTCCGGTATCTAAGCCA+TGG 0.550001 1_1:+59751872 Msa0032570:CDS
AGACTAATTATAGCTTCTGA+TGG 0.551958 1_1:+59750839 Msa0032570:CDS
TCTAAGGTCAAGAGGGCTGA+AGG 0.552829 1_1:+59751049 Msa0032570:CDS
TGTCACCAATCGATCTAGAA+AGG 0.554127 1_1:-59750644 None:intergenic
GAGAGAAACAACACCTCCCT+GGG 0.554466 1_1:-59750406 None:intergenic
AGGGAGCGGGTTACAGCCAG+TGG 0.559334 1_1:+59750468 Msa0032570:CDS
GGAAGAAAATGTAGAAGAGA+GGG 0.560448 1_1:+59750449 Msa0032570:CDS
CACTCCGGTATCTAAGCCAT+GGG 0.564768 1_1:+59751873 Msa0032570:CDS
AAGGTGTAAGCAGGGAGGAA+TGG 0.566433 1_1:+59750140 Msa0032570:CDS
CTAGAAAGGCACAATCCACC+AGG 0.566834 1_1:-59750630 None:intergenic
CCGATGCATATTAGATACCC+AGG 0.567386 1_1:+59750389 Msa0032570:CDS
TCTTCTGACAGTTTGTGCAG+AGG 0.570739 1_1:-59751906 None:intergenic
TTGTGCCTTTCTAGATCGAT+TGG 0.574242 1_1:+59750639 Msa0032570:CDS
CACCAGATGGTTGATCTGCT+AGG 0.577655 1_1:-59751819 None:intergenic
AGGTACACCACGACATGACT+TGG 0.578313 1_1:-59750894 None:intergenic
CAGGAAGCTCTAAGGTCAAG+AGG 0.580501 1_1:+59751041 Msa0032570:intron
ACCTTCACTTGCTTAACATG+TGG 0.582513 1_1:-59750696 None:intergenic
CAGAGGAATGGACCTTCCCA+TGG 0.585428 1_1:-59751889 None:intergenic
TCTCAAAACTCGACAAACAA+AGG 0.587947 1_1:+59751188 Msa0032570:CDS
TGCTTGGTTCTACATTTGGG+TGG 0.590730 1_1:+59752050 Msa0032570:three_prime_UTR
ATAACTATACTGTTGTTGCT+CGG 0.593054 1_1:-59751964 None:intergenic
CTTGACAGTTGATCACAGAC+TGG 0.593422 1_1:+59750428 Msa0032570:CDS
AAGAAGAAAGACGCCATGGA+AGG 0.594109 1_1:+59751925 Msa0032570:CDS
ATCGATTGGTGACACCGATG+TGG 0.601496 1_1:+59750653 Msa0032570:CDS
GCAGATCAACCATCTGGTGA+TGG 0.602774 1_1:+59751823 Msa0032570:CDS
AAGCATTGCTCACACTGCCA+AGG 0.610583 1_1:-59752032 None:intergenic
TACCGGAGTGATAAAATGAT+CGG 0.613081 1_1:-59751860 None:intergenic
CATCACCTTAACCACCAGCT+TGG 0.613564 1_1:-59750930 None:intergenic
GAAATGTTGACTTATAAACT+AGG 0.614349 1_1:+59749504 Msa0032570:five_prime_UTR
CCACCAGGCCAACAGCGAAG+AGG 0.616089 1_1:-59750615 None:intergenic
TGCAACACAGGATAGTCTGA+AGG 0.617016 1_1:-59751104 None:intergenic
TCGATTGGTGACACCGATGT+GGG 0.617709 1_1:+59750654 Msa0032570:CDS
AAGGTTGCCAACGGAACAAG+TGG 0.618923 1_1:-59749560 None:intergenic
ATATACCATTATGCCCGTCA+AGG 0.625718 1_1:-59750060 None:intergenic
GAGCGGGTTACAGCCAGTGG+TGG 0.626341 1_1:+59750471 Msa0032570:CDS
AGGAAGCTCTAAGGTCAAGA+GGG 0.627250 1_1:+59751042 Msa0032570:intron
ACCACATGTTAAGCAAGTGA+AGG 0.627901 1_1:+59750695 Msa0032570:CDS
GTCATGAGTGCCATACCGCA+AGG 0.630194 1_1:+59750121 Msa0032570:CDS
TCAGAAGAAGAAAGACGCCA+TGG 0.630309 1_1:+59751921 Msa0032570:CDS
CCCAACAGATGCAACTGTAA+CGG 0.630410 1_1:-59750361 None:intergenic
CGGCCGATCAAGGTTGCCAA+CGG 0.631386 1_1:-59749569 None:intergenic
TCCTAGCTTTCAAATGCTGG+CGG 0.631854 1_1:+59750815 Msa0032570:intron
CCGGTATCTAAGCCATGGGA+AGG 0.632107 1_1:+59751877 Msa0032570:CDS
TACAGAAATGTTGTATACAG+AGG 0.632924 1_1:-59748891 None:intergenic
AAAGCGACAACTAGAGCCCG+AGG 0.640273 1_1:-59750175 None:intergenic
CAACGTATTCGGAGGCAATG+AGG 0.642352 1_1:+59750509 Msa0032570:CDS
CGATGCATATTAGATACCCA+GGG 0.643134 1_1:+59750390 Msa0032570:CDS
ACTGGTAAACCATCACCAGA+TGG 0.656343 1_1:-59751832 None:intergenic
AAGTGTGTGTGTGACAAATG+AGG 0.666861 1_1:+59752083 Msa0032570:three_prime_UTR
CATACCGCAAGGTGTAAGCA+GGG 0.674683 1_1:+59750132 Msa0032570:CDS
GGAAGACTCAACGTATTCGG+AGG 0.678380 1_1:+59750501 Msa0032570:CDS
AACGATATACTCTCCCACAT+CGG 0.681964 1_1:-59750667 None:intergenic
AGAACTTGCTGCCAAGCTGG+TGG 0.683075 1_1:+59750919 Msa0032570:CDS
ACGGCTGCCAAGTCATGTCG+TGG 0.691102 1_1:+59750887 Msa0032570:CDS
TGTTGTATACAGAGGAAACG+CGG 0.694406 1_1:-59748883 None:intergenic
AAGGAATAATATCTACAACA+AGG 0.708500 1_1:-59751085 None:intergenic
ACCGCAAGGTGTAAGCAGGG+AGG 0.718559 1_1:+59750135 Msa0032570:CDS
GTTCTGCAATGCTTACAGAG+AGG 0.751165 1_1:+59751261 Msa0032570:CDS
TGCATATTAGATACCCAGGG+AGG 0.753250 1_1:+59750393 Msa0032570:CDS
CTGCTAGGCAAACTGCACAG+CGG 0.759343 1_1:-59751804 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAATAATTGTTTTTTA+AGG - chr1_1:59748842-59748861 None:intergenic 10.0%
!! ATAAAAAGATAAATAAAATC+TGG - chr1_1:59749079-59749098 None:intergenic 10.0%
!!! ATAAATATATTGATTTTGTT+TGG + chr1_1:59749463-59749482 Msa0032570:intron 10.0%
!!! TTGAAAATGCATTTTATAAA+TGG + chr1_1:59751467-59751486 Msa0032570:intron 15.0%
!!! TCAATTTTTACTTTTTACAT+CGG - chr1_1:59751652-59751671 None:intergenic 15.0%
!!! ATATTTGTATAATTTTTCCT+TGG + chr1_1:59752015-59752034 Msa0032570:three_prime_UTR 15.0%
!!! CTATGATTAATTTGATTCTA+TGG + chr1_1:59749279-59749298 Msa0032570:intron 20.0%
!! ATAAGATCTTATGAATGAAT+TGG + chr1_1:59749861-59749880 Msa0032570:intron 20.0%
!!! TAAAATTTAATTTTACCTGC+AGG + chr1_1:59750291-59750310 Msa0032570:intron 20.0%
!!! AAAATTTAATTTTACCTGCA+GGG + chr1_1:59750292-59750311 Msa0032570:intron 20.0%
!! TAAGGAAGAAATTATATGAA+AGG - chr1_1:59750557-59750576 None:intergenic 20.0%
!! AAGAACTACTAAACTTTAAA+AGG - chr1_1:59751329-59751348 None:intergenic 20.0%
!!! CATTTGATTATGAATCTTTT+TGG - chr1_1:59751594-59751613 None:intergenic 20.0%
!!! TTTTTGTTTTCTTAAAATGC+AGG + chr1_1:59751740-59751759 Msa0032570:intron 20.0%
!!! TTAACATTACACTTTTTGTT+TGG + chr1_1:59752267-59752286 Msa0032570:three_prime_UTR 20.0%
!!! TATATTGATTTGATTGCTAA+CGG + chr1_1:59752298-59752317 Msa0032570:three_prime_UTR 20.0%
! AAATGAAGAAGAAAAACTCA+CGG - chr1_1:59749040-59749059 None:intergenic 25.0%
!! AGAATGTTGATTATTATAGC+TGG - chr1_1:59749224-59749243 None:intergenic 25.0%
! AATTTGATTCTATGGAAGTA+AGG + chr1_1:59749287-59749306 Msa0032570:intron 25.0%
!! TAGATCTATTAAGAGTCTTT+TGG + chr1_1:59749420-59749439 Msa0032570:intron 25.0%
!!! TTGATTTTGTTTGGATGATT+AGG + chr1_1:59749472-59749491 Msa0032570:intron 25.0%
!!! GAAATGTTGACTTATAAACT+AGG + chr1_1:59749504-59749523 Msa0032570:five_prime_UTR 25.0%
! ATTGATATCATAACCATTGT+GGG - chr1_1:59750018-59750037 None:intergenic 25.0%
! TATTGATATCATAACCATTG+TGG - chr1_1:59750019-59750038 None:intergenic 25.0%
! CAATGGTTATGATATCAATA+AGG + chr1_1:59750019-59750038 Msa0032570:intron 25.0%
! ATCAATAAGGAATTCTTGAT+TGG + chr1_1:59750032-59750051 Msa0032570:intron 25.0%
!! AGTTTTCTTTGTATCCTTAT+TGG + chr1_1:59750240-59750259 Msa0032570:intron 25.0%
!!! AAATTTAATTTTACCTGCAG+GGG + chr1_1:59750293-59750312 Msa0032570:intron 25.0%
!! TTTGGTTCCTTAATATGTTT+AGG + chr1_1:59750587-59750606 Msa0032570:intron 25.0%
! TTCCTTAATATGTTTAGGTA+GGG + chr1_1:59750592-59750611 Msa0032570:intron 25.0%
!!! TCTATTGTTTTAACTTGAAC+AGG + chr1_1:59751022-59751041 Msa0032570:intron 25.0%
! AAGGAATAATATCTACAACA+AGG - chr1_1:59751088-59751107 None:intergenic 25.0%
! TGATTGGAACAAAAATGTTT+AGG + chr1_1:59751368-59751387 Msa0032570:intron 25.0%
! GATTGGAACAAAAATGTTTA+GGG + chr1_1:59751369-59751388 Msa0032570:intron 25.0%
!!! GGTTGTTGCAAATTATTTTT+TGG + chr1_1:59752141-59752160 Msa0032570:three_prime_UTR 25.0%
TACAGAAATGTTGTATACAG+AGG - chr1_1:59748894-59748913 None:intergenic 30.0%
!! TTTGCTCTTTGATTTTCGAT+TGG - chr1_1:59748917-59748936 None:intergenic 30.0%
AAGAGTTGAAAAACCCTAAT+GGG - chr1_1:59748969-59748988 None:intergenic 30.0%
!! CAGAATTTTGAGTATGAGAA+AGG - chr1_1:59748994-59749013 None:intergenic 30.0%
AATGAAGAAGAAAAACTCAC+GGG - chr1_1:59749039-59749058 None:intergenic 30.0%
AGTATCTTAGCTTAGTTAGT+TGG + chr1_1:59749147-59749166 Msa0032570:intron 30.0%
! TTTTGTACTTCTAGACTCAA+TGG + chr1_1:59749351-59749370 Msa0032570:intron 30.0%
TGAGTAAGACTGAATTATCA+AGG + chr1_1:59749528-59749547 Msa0032570:CDS 30.0%
TTAGTGAAATGTTTGATGAG+AGG + chr1_1:59749803-59749822 Msa0032570:intron 30.0%
!! TTTGATGAGAGGTATTTGAT+TGG + chr1_1:59749814-59749833 Msa0032570:intron 30.0%
TAAGAAAGATACATTCCCTT+TGG - chr1_1:59749909-59749928 None:intergenic 30.0%
AAAGGGAATGTATCTTTCTT+AGG + chr1_1:59749908-59749927 Msa0032570:intron 30.0%
GACATGGAATTTCAGAAAAA+AGG + chr1_1:59750208-59750227 Msa0032570:CDS 30.0%
!!! CTTCCTTATCTAACTTGTTT+TGG + chr1_1:59750569-59750588 Msa0032570:intron 30.0%
GAACCAAAACAAGTTAGATA+AGG - chr1_1:59750575-59750594 None:intergenic 30.0%
GTTCCTTAATATGTTTAGGT+AGG + chr1_1:59750591-59750610 Msa0032570:intron 30.0%
GTAAACACCTTCACATAAAA+GGG - chr1_1:59750757-59750776 None:intergenic 30.0%
AGTAAACACCTTCACATAAA+AGG - chr1_1:59750758-59750777 None:intergenic 30.0%
AGACTAATTATAGCTTCTGA+TGG + chr1_1:59750839-59750858 Msa0032570:CDS 30.0%
TTATAGCTTCTGATGGAATT+TGG + chr1_1:59750846-59750865 Msa0032570:CDS 30.0%
TATAGCTTCTGATGGAATTT+GGG + chr1_1:59750847-59750866 Msa0032570:CDS 30.0%
GAATTCAAGTCTCATTATGT+TGG + chr1_1:59750984-59751003 Msa0032570:intron 30.0%
AATAATATCTACAACAAGGC+AGG - chr1_1:59751084-59751103 None:intergenic 30.0%
AGCACATTATGTTTCTTTCT+TGG - chr1_1:59751143-59751162 None:intergenic 30.0%
!! AACTAGAGAAAGTGCATTTT+CGG - chr1_1:59751546-59751565 None:intergenic 30.0%
!!! ATGTCCTTATTTTCCCATTT+TGG + chr1_1:59751693-59751712 Msa0032570:intron 30.0%
!!! TGTCCTTATTTTCCCATTTT+GGG + chr1_1:59751694-59751713 Msa0032570:intron 30.0%
ATACCAGAATTTGTATTGGA+AGG - chr1_1:59751781-59751800 None:intergenic 30.0%
GTGAATACCAGAATTTGTAT+TGG - chr1_1:59751785-59751804 None:intergenic 30.0%
ATAACTATACTGTTGTTGCT+CGG - chr1_1:59751967-59751986 None:intergenic 30.0%
! TAATTTGCAACAACCTTCAA+TGG - chr1_1:59752136-59752155 None:intergenic 30.0%
GAAGAGTTGAAAAACCCTAA+TGG - chr1_1:59748970-59748989 None:intergenic 35.0%
ATGAGCACTATAGTGAAACA+TGG - chr1_1:59749318-59749337 None:intergenic 35.0%
! TTTTTCCATTTCGTAGCTCA+CGG - chr1_1:59749592-59749611 None:intergenic 35.0%
TGAGCTACGAAATGGAAAAA+CGG + chr1_1:59749592-59749611 Msa0032570:CDS 35.0%
!! AACGTCATTTTCTGTTTTCG+CGG + chr1_1:59749715-59749734 Msa0032570:CDS 35.0%
! ACATTTTCTCAGTGATCAAG+TGG + chr1_1:59749978-59749997 Msa0032570:intron 35.0%
GCTACGTTTGTGATTATTGA+TGG + chr1_1:59750333-59750352 Msa0032570:CDS 35.0%
CGTTTGTGATTATTGATGGA+TGG + chr1_1:59750337-59750356 Msa0032570:CDS 35.0%
TGGAAGAAAATGTAGAAGAG+AGG + chr1_1:59750448-59750467 Msa0032570:CDS 35.0%
GGAAGAAAATGTAGAAGAGA+GGG + chr1_1:59750449-59750468 Msa0032570:CDS 35.0%
ACTTGCTTAACATGTGGTAT+TGG - chr1_1:59750693-59750712 None:intergenic 35.0%
!!! GTGAGTTGGTTTCATTTTCA+TGG + chr1_1:59750717-59750736 Msa0032570:intron 35.0%
!! TTCTAAAGTTCAATGCTCAC+TGG + chr1_1:59750777-59750796 Msa0032570:intron 35.0%
AAAGCTAGGAAACAAATCAG+GGG - chr1_1:59750805-59750824 None:intergenic 35.0%
GAAAGCTAGGAAACAAATCA+GGG - chr1_1:59750806-59750825 None:intergenic 35.0%
TGAAAGCTAGGAAACAAATC+AGG - chr1_1:59750807-59750826 None:intergenic 35.0%
GGATACTTTGTCTTCTGATA+CGG + chr1_1:59750868-59750887 Msa0032570:CDS 35.0%
! GTTAAGGTGATGGTTATTTG+TGG + chr1_1:59750941-59750960 Msa0032570:intron 35.0%
!!! GGCAGTGTTCTTATTTTTTG+AGG + chr1_1:59750962-59750981 Msa0032570:intron 35.0%
TCTCAAAACTCGACAAACAA+AGG + chr1_1:59751188-59751207 Msa0032570:CDS 35.0%
ACTAATAAGCTTTCTGCAGT+TGG + chr1_1:59751212-59751231 Msa0032570:CDS 35.0%
! TTCCAATCACTTGTTGATGA+TGG - chr1_1:59751357-59751376 None:intergenic 35.0%
! TGTTTAGGGTCTGTTTACTT+CGG + chr1_1:59751383-59751402 Msa0032570:intron 35.0%
ACACCCAAAATGGGAAAATA+AGG - chr1_1:59751700-59751719 None:intergenic 35.0%
CACATGAATACACCCAAAAT+GGG - chr1_1:59751709-59751728 None:intergenic 35.0%
ACACATGAATACACCCAAAA+TGG - chr1_1:59751710-59751729 None:intergenic 35.0%
! GTTTTCTTAAAATGCAGGCT+AGG + chr1_1:59751745-59751764 Msa0032570:intron 35.0%
GTTCCTTCCAATACAAATTC+TGG + chr1_1:59751775-59751794 Msa0032570:CDS 35.0%
! ATAAAATGATCGGTGTTCAC+TGG - chr1_1:59751853-59751872 None:intergenic 35.0%
TACCGGAGTGATAAAATGAT+CGG - chr1_1:59751863-59751882 None:intergenic 35.0%
!! CAATGCTTGGTTCTACATTT+GGG + chr1_1:59752047-59752066 Msa0032570:three_prime_UTR 35.0%
AGAATTGAGTTAGCTAGGTT+AGG + chr1_1:59752109-59752128 Msa0032570:three_prime_UTR 35.0%
TGTTGTATACAGAGGAAACG+CGG - chr1_1:59748886-59748905 None:intergenic 40.0%
TCTACGCATGTTGAGTGAAT+TGG - chr1_1:59749404-59749423 None:intergenic 40.0%
! GAGAAGCCTTTTGTGAAGTA+TGG + chr1_1:59749614-59749633 Msa0032570:CDS 40.0%
! TCATTTTCTGTTTTCGCGGT+AGG + chr1_1:59749719-59749738 Msa0032570:intron 40.0%
GCGTCAATTTATTCCCACAA+TGG + chr1_1:59750002-59750021 Msa0032570:intron 40.0%
ATATACCATTATGCCCGTCA+AGG - chr1_1:59750063-59750082 None:intergenic 40.0%
TTCCTTCGCAAAAATAGCAG+CGG - chr1_1:59750086-59750105 None:intergenic 40.0%
! CGCTTTTGTGAAAACTGACA+TGG + chr1_1:59750192-59750211 Msa0032570:CDS 40.0%
GAACAAGTACAGCTCCAATA+AGG - chr1_1:59750257-59750276 None:intergenic 40.0%
CTGGGTATCTAATATGCATC+GGG - chr1_1:59750391-59750410 None:intergenic 40.0%
CCTGGGTATCTAATATGCAT+CGG - chr1_1:59750392-59750411 None:intergenic 40.0%
CGATGCATATTAGATACCCA+GGG + chr1_1:59750390-59750409 Msa0032570:CDS 40.0%
AAAATGTAGAAGAGAGGGAG+CGG + chr1_1:59750454-59750473 Msa0032570:CDS 40.0%
GTAGGAAGACTCAACGTATT+CGG + chr1_1:59750498-59750517 Msa0032570:CDS 40.0%
GGCCCTACCTAAACATATTA+AGG - chr1_1:59750597-59750616 None:intergenic 40.0%
TTGTGCCTTTCTAGATCGAT+TGG + chr1_1:59750639-59750658 Msa0032570:CDS 40.0%
TGTCACCAATCGATCTAGAA+AGG - chr1_1:59750647-59750666 None:intergenic 40.0%
AACGATATACTCTCCCACAT+CGG - chr1_1:59750670-59750689 None:intergenic 40.0%
ACCACATGTTAAGCAAGTGA+AGG + chr1_1:59750695-59750714 Msa0032570:CDS 40.0%
ACCTTCACTTGCTTAACATG+TGG - chr1_1:59750699-59750718 None:intergenic 40.0%
TTAAGCAAGTGAAGGTGAGT+TGG + chr1_1:59750703-59750722 Msa0032570:intron 40.0%
! CTAGTGACCCTTTTATGTGA+AGG + chr1_1:59750747-59750766 Msa0032570:intron 40.0%
! GTTTCCTAGCTTTCAAATGC+TGG + chr1_1:59750812-59750831 Msa0032570:intron 40.0%
AACTTGAACAGGAAGCTCTA+AGG + chr1_1:59751033-59751052 Msa0032570:intron 40.0%
TGTTTCTTTCTTGGAGTTGC+TGG - chr1_1:59751134-59751153 None:intergenic 40.0%
GTGCTTAGTTCACTTCTCTT+TGG + chr1_1:59751158-59751177 Msa0032570:CDS 40.0%
TGTGGAGGAGTTATTTGAAG+AGG + chr1_1:59751238-59751257 Msa0032570:CDS 40.0%
GTGGAGGAGTTATTTGAAGA+GGG + chr1_1:59751239-59751258 Msa0032570:CDS 40.0%
TGCCATCATCAACAAGTGAT+TGG + chr1_1:59751352-59751371 Msa0032570:intron 40.0%
! GTTTAGGGTCTGTTTACTTC+GGG + chr1_1:59751384-59751403 Msa0032570:intron 40.0%
CTGTCATTGAGAGAACATGA+AGG - chr1_1:59751571-59751590 None:intergenic 40.0%
!!! TTACTTTTTACATCGGCAGC+TGG - chr1_1:59751645-59751664 None:intergenic 40.0%
CTTAAAATGCAGGCTAGGTA+AGG + chr1_1:59751750-59751769 Msa0032570:intron 40.0%
! CACCGATCATTTTATCACTC+CGG + chr1_1:59751858-59751877 Msa0032570:CDS 40.0%
!! GCAATGCTTGGTTCTACATT+TGG + chr1_1:59752046-59752065 Msa0032570:three_prime_UTR 40.0%
AAGTGTGTGTGTGACAAATG+AGG + chr1_1:59752083-59752102 Msa0032570:three_prime_UTR 40.0%
GGTTGAGAATTGAGTTAGCT+AGG + chr1_1:59752104-59752123 Msa0032570:three_prime_UTR 40.0%
TGTGCTCTCAACACCCATTA+GGG + chr1_1:59748953-59748972 Msa0032570:five_prime_UTR 45.0%
! GCCTTTTGTGAAGTATGGAC+AGG + chr1_1:59749619-59749638 Msa0032570:CDS 45.0%
GCCTGTCCATACTTCACAAA+AGG - chr1_1:59749623-59749642 None:intergenic 45.0%
TTTGTGAAGTATGGACAGGC+TGG + chr1_1:59749623-59749642 Msa0032570:CDS 45.0%
!! CAGGCTGGTTTGGCAAAAAA+AGG + chr1_1:59749638-59749657 Msa0032570:CDS 45.0%
ACAGATTGCCATAGAGTTCC+TGG + chr1_1:59749683-59749702 Msa0032570:CDS 45.0%
AAGAATCGCCAGGAACTCTA+TGG - chr1_1:59749694-59749713 None:intergenic 45.0%
AATGACGTTGAAGAATCGCC+AGG - chr1_1:59749704-59749723 None:intergenic 45.0%
TTCTGCTTCGAGAGACCAAA+GGG + chr1_1:59749891-59749910 Msa0032570:intron 45.0%
CTTGATTGGCAGATCCTTGA+CGG + chr1_1:59750046-59750065 Msa0032570:intron 45.0%
TTGATTGGCAGATCCTTGAC+GGG + chr1_1:59750047-59750066 Msa0032570:intron 45.0%
!!! ATCCGCTGCTATTTTTGCGA+AGG + chr1_1:59750081-59750100 Msa0032570:CDS 45.0%
ACCGTTACAGTTGCATCTGT+TGG + chr1_1:59750360-59750379 Msa0032570:CDS 45.0%
CCCAACAGATGCAACTGTAA+CGG - chr1_1:59750364-59750383 None:intergenic 45.0%
CCGTTACAGTTGCATCTGTT+GGG + chr1_1:59750361-59750380 Msa0032570:CDS 45.0%
CGTTACAGTTGCATCTGTTG+GGG + chr1_1:59750362-59750381 Msa0032570:CDS 45.0%
CCGATGCATATTAGATACCC+AGG + chr1_1:59750389-59750408 Msa0032570:CDS 45.0%
TGCATATTAGATACCCAGGG+AGG + chr1_1:59750393-59750412 Msa0032570:CDS 45.0%
CTTGACAGTTGATCACAGAC+TGG + chr1_1:59750428-59750447 Msa0032570:CDS 45.0%
AAATGTAGAAGAGAGGGAGC+GGG + chr1_1:59750455-59750474 Msa0032570:CDS 45.0%
! TCCTAGCTTTCAAATGCTGG+CGG + chr1_1:59750815-59750834 Msa0032570:intron 45.0%
!! AGGAAGCTCTAAGGTCAAGA+GGG + chr1_1:59751042-59751061 Msa0032570:intron 45.0%
TGCAACACAGGATAGTCTGA+AGG - chr1_1:59751107-59751126 None:intergenic 45.0%
! GCTTTCTGCAGTTGGTATTG+TGG + chr1_1:59751220-59751239 Msa0032570:CDS 45.0%
! TTCTGCAGTTGGTATTGTGG+AGG + chr1_1:59751223-59751242 Msa0032570:CDS 45.0%
GTTCTGCAATGCTTACAGAG+AGG + chr1_1:59751261-59751280 Msa0032570:CDS 45.0%
ACTGGTAAACCATCACCAGA+TGG - chr1_1:59751835-59751854 None:intergenic 45.0%
TGACAGTTTGTGCAGAGGAA+TGG - chr1_1:59751904-59751923 None:intergenic 45.0%
TCTTCTGACAGTTTGTGCAG+AGG - chr1_1:59751909-59751928 None:intergenic 45.0%
TCAGAAGAAGAAAGACGCCA+TGG + chr1_1:59751921-59751940 Msa0032570:CDS 45.0%
AAGAAGAAAGACGCCATGGA+AGG + chr1_1:59751925-59751944 Msa0032570:CDS 45.0%
AAGACGCCATGGAAGGAAAA+AGG + chr1_1:59751932-59751951 Msa0032570:CDS 45.0%
! TCAGGACCTTTTTCCTTCCA+TGG - chr1_1:59751941-59751960 None:intergenic 45.0%
!! TGCTTGGTTCTACATTTGGG+TGG + chr1_1:59752050-59752069 Msa0032570:three_prime_UTR 45.0%
AGCTAGGTTAGGTCCATTGA+AGG + chr1_1:59752120-59752139 Msa0032570:three_prime_UTR 45.0%
CTGTGCTCTCAACACCCATT+AGG + chr1_1:59748952-59748971 Msa0032570:five_prime_UTR 50.0%
GAAGCAACCACTTGTTCCGT+TGG + chr1_1:59749553-59749572 Msa0032570:CDS 50.0%
AAGGTTGCCAACGGAACAAG+TGG - chr1_1:59749563-59749582 None:intergenic 50.0%
GTTCCGTTGGCAACCTTGAT+CGG + chr1_1:59749566-59749585 Msa0032570:CDS 50.0%
! GAAGTATGGACAGGCTGGTT+TGG + chr1_1:59749628-59749647 Msa0032570:CDS 50.0%
GTTCTGCTTCGAGAGACCAA+AGG + chr1_1:59749890-59749909 Msa0032570:intron 50.0%
! CAGATCCTTGACGGGCATAA+TGG + chr1_1:59750055-59750074 Msa0032570:intron 50.0%
CATACCGCAAGGTGTAAGCA+GGG + chr1_1:59750132-59750151 Msa0032570:CDS 50.0%
AAGGTGTAAGCAGGGAGGAA+TGG + chr1_1:59750140-59750159 Msa0032570:CDS 50.0%
! AATGGCTTCAAGCACTGCCT+CGG + chr1_1:59750158-59750177 Msa0032570:CDS 50.0%
TTACCTGCAGGGGAAACTTC+TGG + chr1_1:59750303-59750322 Msa0032570:intron 50.0%
GAGAGAAACAACACCTCCCT+GGG - chr1_1:59750409-59750428 None:intergenic 50.0%
AGAGAGAAACAACACCTCCC+TGG - chr1_1:59750410-59750429 None:intergenic 50.0%
TCTTCCTACTTCACCACCAC+TGG - chr1_1:59750487-59750506 None:intergenic 50.0%
GGAAGACTCAACGTATTCGG+AGG + chr1_1:59750501-59750520 Msa0032570:CDS 50.0%
CAACGTATTCGGAGGCAATG+AGG + chr1_1:59750509-59750528 Msa0032570:CDS 50.0%
! CTAGAAAGGCACAATCCACC+AGG - chr1_1:59750633-59750652 None:intergenic 50.0%
! ATCGATTGGTGACACCGATG+TGG + chr1_1:59750653-59750672 Msa0032570:CDS 50.0%
! TCGATTGGTGACACCGATGT+GGG + chr1_1:59750654-59750673 Msa0032570:CDS 50.0%
TCCGCCAGCATTTGAAAGCT+AGG - chr1_1:59750819-59750838 None:intergenic 50.0%
AGGTACACCACGACATGACT+TGG - chr1_1:59750897-59750916 None:intergenic 50.0%
CATCACCTTAACCACCAGCT+TGG - chr1_1:59750933-59750952 None:intergenic 50.0%
CAAGCTGGTGGTTAAGGTGA+TGG + chr1_1:59750931-59750950 Msa0032570:intron 50.0%
!! CAGGAAGCTCTAAGGTCAAG+AGG + chr1_1:59751041-59751060 Msa0032570:intron 50.0%
! TCTAAGGTCAAGAGGGCTGA+AGG + chr1_1:59751049-59751068 Msa0032570:CDS 50.0%
TGCCTAGCAGATCAACCATC+TGG + chr1_1:59751817-59751836 Msa0032570:CDS 50.0%
CACCAGATGGTTGATCTGCT+AGG - chr1_1:59751822-59751841 None:intergenic 50.0%
GCAGATCAACCATCTGGTGA+TGG + chr1_1:59751823-59751842 Msa0032570:CDS 50.0%
TCACTCCGGTATCTAAGCCA+TGG + chr1_1:59751872-59751891 Msa0032570:CDS 50.0%
CACTCCGGTATCTAAGCCAT+GGG + chr1_1:59751873-59751892 Msa0032570:CDS 50.0%
CCTTCCCATGGCTTAGATAC+CGG - chr1_1:59751880-59751899 None:intergenic 50.0%
ACTGTTGTTGCTCGGACTTC+AGG - chr1_1:59751959-59751978 None:intergenic 50.0%
! AAGCATTGCTCACACTGCCA+AGG - chr1_1:59752035-59752054 None:intergenic 50.0%
TTGGCAGTGTGAGCAATGCT+TGG + chr1_1:59752034-59752053 Msa0032570:three_prime_UTR 50.0%
!! TTTGGCTTGTGCTCAGCTGT+TGG + chr1_1:59752159-59752178 Msa0032570:three_prime_UTR 50.0%
ATCGGCCGTGAGCTACGAAA+TGG + chr1_1:59749584-59749603 Msa0032570:CDS 55.0%
GTCATGAGTGCCATACCGCA+AGG + chr1_1:59750121-59750140 Msa0032570:CDS 55.0%
CCTGCTTACACCTTGCGGTA+TGG - chr1_1:59750134-59750153 None:intergenic 55.0%
CCATACCGCAAGGTGTAAGC+AGG + chr1_1:59750131-59750150 Msa0032570:CDS 55.0%
TCCTCCCTGCTTACACCTTG+CGG - chr1_1:59750139-59750158 None:intergenic 55.0%
! ATGGCTTCAAGCACTGCCTC+GGG + chr1_1:59750159-59750178 Msa0032570:CDS 55.0%
AAAGCGACAACTAGAGCCCG+AGG - chr1_1:59750178-59750197 None:intergenic 55.0%
GTTCCAGAAGTTTCCCCTGC+AGG - chr1_1:59750309-59750328 None:intergenic 55.0%
! ACAGCCAGTGGTGGTGAAGT+AGG + chr1_1:59750480-59750499 Msa0032570:CDS 55.0%
AGCTTGGCAGCAAGTTCTGC+AGG - chr1_1:59750917-59750936 None:intergenic 55.0%
!! TGCAGAACTTGCTGCCAAGC+TGG + chr1_1:59750916-59750935 Msa0032570:CDS 55.0%
!! AGAACTTGCTGCCAAGCTGG+TGG + chr1_1:59750919-59750938 Msa0032570:CDS 55.0%
TGCTGCCAAGCTGGTGGTTA+AGG + chr1_1:59750925-59750944 Msa0032570:CDS 55.0%
GTTGCTGGCATCTGCAACAC+AGG - chr1_1:59751119-59751138 None:intergenic 55.0%
CTGCTAGGCAAACTGCACAG+CGG - chr1_1:59751807-59751826 None:intergenic 55.0%
CCGGTATCTAAGCCATGGGA+AGG + chr1_1:59751877-59751896 Msa0032570:CDS 55.0%
CAGAGGAATGGACCTTCCCA+TGG - chr1_1:59751892-59751911 None:intergenic 55.0%
CGGCCGATCAAGGTTGCCAA+CGG - chr1_1:59749572-59749591 None:intergenic 60.0%
TCGTAGCTCACGGCCGATCA+AGG - chr1_1:59749582-59749601 None:intergenic 60.0%
ACCGCAAGGTGTAAGCAGGG+AGG + chr1_1:59750135-59750154 Msa0032570:CDS 60.0%
AGGTAGGGCCTCTTCGCTGT+TGG + chr1_1:59750607-59750626 Msa0032570:intron 60.0%
ACGGCTGCCAAGTCATGTCG+TGG + chr1_1:59750887-59750906 Msa0032570:CDS 60.0%
!! AGGGAGCGGGTTACAGCCAG+TGG + chr1_1:59750468-59750487 Msa0032570:CDS 65.0%
!! GAGCGGGTTACAGCCAGTGG+TGG + chr1_1:59750471-59750490 Msa0032570:CDS 65.0%
CCACCAGGCCAACAGCGAAG+AGG - chr1_1:59750618-59750637 None:intergenic 65.0%
! CCTCTTCGCTGTTGGCCTGG+TGG + chr1_1:59750615-59750634 Msa0032570:CDS 65.0%
GGGCCTCTTCGCTGTTGGCC+TGG + chr1_1:59750612-59750631 Msa0032570:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 59748826 59752321 59748826 ID=Msa0032570;Name=Msa0032570
chr1_1 mRNA 59748826 59752321 59748826 ID=Msa0032570-mRNA-1;Parent=Msa0032570;Name=Msa0032570-mRNA-1;_AED=0.09;_eAED=0.09;_QI=246|1|1|1|0.85|0.75|8|361|423
chr1_1 exon 59748826 59749038 59748826 ID=Msa0032570-mRNA-1:exon:18470;Parent=Msa0032570-mRNA-1
chr1_1 exon 59749494 59749736 59749494 ID=Msa0032570-mRNA-1:exon:18471;Parent=Msa0032570-mRNA-1
chr1_1 exon 59750058 59750229 59750058 ID=Msa0032570-mRNA-1:exon:18472;Parent=Msa0032570-mRNA-1
chr1_1 exon 59750313 59750530 59750313 ID=Msa0032570-mRNA-1:exon:18473;Parent=Msa0032570-mRNA-1
chr1_1 exon 59750609 59750716 59750609 ID=Msa0032570-mRNA-1:exon:18474;Parent=Msa0032570-mRNA-1
chr1_1 exon 59750821 59750946 59750821 ID=Msa0032570-mRNA-1:exon:18475;Parent=Msa0032570-mRNA-1
chr1_1 exon 59751044 59751282 59751044 ID=Msa0032570-mRNA-1:exon:18476;Parent=Msa0032570-mRNA-1
chr1_1 exon 59751762 59752321 59751762 ID=Msa0032570-mRNA-1:exon:18477;Parent=Msa0032570-mRNA-1
chr1_1 five_prime_UTR 59748826 59749038 59748826 ID=Msa0032570-mRNA-1:five_prime_utr;Parent=Msa0032570-mRNA-1
chr1_1 five_prime_UTR 59749494 59749526 59749494 ID=Msa0032570-mRNA-1:five_prime_utr;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59749527 59749736 59749527 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59750058 59750229 59750058 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59750313 59750530 59750313 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59750609 59750716 59750609 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59750821 59750946 59750821 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59751044 59751282 59751044 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 CDS 59751762 59751960 59751762 ID=Msa0032570-mRNA-1:cds;Parent=Msa0032570-mRNA-1
chr1_1 three_prime_UTR 59751961 59752321 59751961 ID=Msa0032570-mRNA-1:three_prime_utr;Parent=Msa0032570-mRNA-1
Gene Sequence

>Msa0032570

ATGAGTAAGACTGAATTATCAAGGATGAAGCAACCACTTGTTCCGTTGGCAACCTTGATCGGCCGTGAGCTACGAAATGGAAAAACGGAGAAGCCTTTTGTGAAGTATGGACAGGCTGGTTTGGCAAAAAAAGGAGAAGATTACTTTCTAATCAAGACAGATTGCCATAGAGTTCCTGGCGATTCTTCAACGTCATTTTCTGTTTTCGCGATCCTTGACGGGCATAATGGTATATCCGCTGCTATTTTTGCGAAGGAAAACATAATAAACAATGTCATGAGTGCCATACCGCAAGGTGTAAGCAGGGAGGAATGGCTTCAAGCACTGCCTCGGGCTCTAGTTGTCGCTTTTGTGAAAACTGACATGGAATTTCAGAAAAAAGGGGAAACTTCTGGAACGACAGCTACGTTTGTGATTATTGATGGATGGACCGTTACAGTTGCATCTGTTGGGGATTCCCGATGCATATTAGATACCCAGGGAGGTGTTGTTTCTCTCTTGACAGTTGATCACAGACTGGAAGAAAATGTAGAAGAGAGGGAGCGGGTTACAGCCAGTGGTGGTGAAGTAGGAAGACTCAACGTATTCGGAGGCAATGAGGTAGGGCCTCTTCGCTGTTGGCCTGGTGGATTGTGCCTTTCTAGATCGATTGGTGACACCGATGTGGGAGAGTATATCGTTCCAATACCACATGTTAAGCAAGTGAAGCTTTCAAATGCTGGCGGAAGACTAATTATAGCTTCTGATGGAATTTGGGATACTTTGTCTTCTGATACGGCTGCCAAGTCATGTCGTGGTGTACCTGCAGAACTTGCTGCCAAGCTGGTGGTTAAGGAAGCTCTAAGGTCAAGAGGGCTGAAGGATGATACAACCTGCCTTGTTGTAGATATTATTCCTTCAGACTATCCTGTGTTGCAGATGCCAGCAACTCCAAGAAAGAAACATAATGTGCTTAGTTCACTTCTCTTTGGAAAGAAATCTCAAAACTCGACAAACAAAGGAACTAATAAGCTTTCTGCAGTTGGTATTGTGGAGGAGTTATTTGAAGAGGGTTCTGCAATGCTTACAGAGAGGCTAGGTAAGGATGTTCCTTCCAATACAAATTCTGGTATTCACCGCTGTGCAGTTTGCCTAGCAGATCAACCATCTGGTGATGGTTTACCAGTGAACACCGATCATTTTATCACTCCGGTATCTAAGCCATGGGAAGGTCCATTCCTCTGCACAAACTGTCAGAAGAAGAAAGACGCCATGGAAGGAAAAAGGTCCTGA
Protein sequence

>Msa0032570

MSKTELSRMKQPLVPLATLIGRELRNGKTEKPFVKYGQAGLAKKGEDYFLIKTDCHRVPGDSSTSFSVFAILDGHNGISAAIFAKENIINNVMSAIPQGVSREEWLQALPRALVVAFVKTDMEFQKKGETSGTTATFVIIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENVEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEYIVPIPHVKQVKLSNAGGRLIIASDGIWDTLSSDTAAKSCRGVPAELAAKLVVKEALRSRGLKDDTTCLVVDIIPSDYPVLQMPATPRKKHNVLSSLLFGKKSQNSTNKGTNKLSAVGIVEELFEEGSAMLTERLGKDVPSNTNSGIHRCAVCLADQPSGDGLPVNTDHFITPVSKPWEGPFLCTNCQKKKDAMEGKRS*