AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0780900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780900 MtrunA17_Chr5g0441381 96.250 240 8 1 1 239 1 240 2.81e-172 473
Msa0780900 MtrunA17_Chr7g0246301 40.940 149 78 5 9 155 8 148 5.32e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780900 AT1G08500.1 59.278 194 73 3 13 200 7 200 9.85e-78 234

Find 58 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTATGTTCCATTATTCT+TGG 0.181685 5_2:+63084682 None:intergenic
AAACCGACATGAAATGAAAT+AGG 0.222292 5_2:-63084887 Msa0780900:five_prime_UTR
TCCCAAGCTGAATTCTTTCT+TGG 0.237475 5_2:+63084504 None:intergenic
TCTCCGGCTAGTCCGATTTC+TGG 0.249790 5_2:-63082851 Msa0780900:CDS
AAATTTCACAACATGTTATA+TGG 0.271985 5_2:-63082692 Msa0780900:CDS
TTCCTTAGGATGGTTGAAAT+TGG 0.295594 5_2:+63082793 None:intergenic
CAACTGTTAATTATCAGAAA+TGG 0.300214 5_2:-63084531 Msa0780900:CDS
ATTATTATAAAATAAATAAA+AGG 0.310077 5_2:+63082619 None:intergenic
TTCTATGTTCCATTATTCTT+GGG 0.311538 5_2:+63084683 None:intergenic
TTTCGCTCACCCAAGAATAA+TGG 0.378679 5_2:-63084692 Msa0780900:exon
TCATCGTCACTATCTTCCTT+AGG 0.388615 5_2:+63082779 None:intergenic
TCATGTTTCTTAGGTTCTGT+TGG 0.394187 5_2:-63084613 Msa0780900:CDS
TACACAGTGGGAGATTCTTT+AGG 0.400880 5_2:-63084577 Msa0780900:CDS
CTTTAACATCGAAACAGAAC+TGG 0.404508 5_2:+63082721 None:intergenic
AACTGTTAATTATCAGAAAT+GGG 0.420262 5_2:-63084530 Msa0780900:CDS
AAAGGAACTGCCACTGTGAC+TGG 0.433267 5_2:+63083013 None:intergenic
TTATACAACAATAACATAGA+TGG 0.440206 5_2:+63082531 None:intergenic
TTGACGTGAGATTGTGAGCT+AGG 0.465493 5_2:-63082559 Msa0780900:three_prime_UTR
AACCGACATGAAATGAAATA+GGG 0.467134 5_2:-63084886 Msa0780900:five_prime_UTR
GTTGCCCCTTCTTTCAGTAA+AGG 0.467797 5_2:+63082995 None:intergenic
GACTCATCGTCACCAGAAAT+CGG 0.470529 5_2:+63082839 None:intergenic
GCAGTTCCTTTACTGAAAGA+AGG 0.475413 5_2:-63083001 Msa0780900:CDS
GGTGAGCGAAATGTGAATAA+TGG 0.480913 5_2:+63084704 None:intergenic
AAATCACATGTAACCATAGC+TGG 0.482015 5_2:-63084813 Msa0780900:five_prime_UTR
TGACAAAGATAAAGATAAAG+AGG 0.482717 5_2:-63082747 Msa0780900:CDS
GCCAAGAAAGAATTCAGCTT+GGG 0.483597 5_2:-63084505 Msa0780900:CDS
TTGGAAGGAATATTTGTGTC+TGG 0.492247 5_2:+63082812 None:intergenic
CGTCACTATCTTCCTTAGGA+TGG 0.500515 5_2:+63082783 None:intergenic
GATTCATATGCTGCCAGCTA+TGG 0.501233 5_2:+63084800 None:intergenic
TTCTTCTCTAGTGATTATGA+TGG 0.501250 5_2:-63082968 Msa0780900:CDS
GTCGGAAGATTCTCCATCTC+CGG 0.504591 5_2:-63082867 Msa0780900:CDS
CAGTGGGAGATTCTTTAGGC+TGG 0.508955 5_2:-63084573 Msa0780900:CDS
ACGGATCAACTGATGACCAT+TGG 0.511597 5_2:+63083053 None:intergenic
TTAGGATGGTTGAAATTGGA+AGG 0.515170 5_2:+63082797 None:intergenic
CATTTCTGATAATTAACAGT+TGG 0.517247 5_2:+63084532 None:intergenic
CAGTTCCTTTACTGAAAGAA+GGG 0.524215 5_2:-63083000 Msa0780900:CDS
TTCAAAATAAATGTGACTCA+TGG 0.525741 5_2:-63082920 Msa0780900:CDS
TTCCAATTTCAACCATCCTA+AGG 0.526093 5_2:-63082795 Msa0780900:CDS
CTCCCTATTTCATTTCATGT+CGG 0.528285 5_2:+63084884 None:intergenic
TATATGTTTGAACAAGAGAG+TGG 0.543824 5_2:+63083131 None:intergenic
AGTGGATGAGAATGTAATGT+AGG 0.544501 5_2:+63084778 None:intergenic
TCACCAGAAATCGGACTAGC+CGG 0.553642 5_2:+63082848 None:intergenic
ATAATCACATTGTTTGTATG+TGG 0.560805 5_2:+63083102 None:intergenic
TGCCAAGAAAGAATTCAGCT+TGG 0.561777 5_2:-63084506 Msa0780900:CDS
GAAATCGGACTAGCCGGAGA+TGG 0.573901 5_2:+63082854 None:intergenic
TGAATAATGGCAACTAGCAG+AGG 0.583195 5_2:+63084717 None:intergenic
GACTGGATGTATATCAGTGT+TGG 0.601352 5_2:+63083030 None:intergenic
ATAAATGTGACTCATGGACA+AGG 0.609850 5_2:-63082914 Msa0780900:CDS
TGACGTGAGATTGTGAGCTA+GGG 0.626083 5_2:-63082558 Msa0780900:three_prime_UTR
TTATGATGATGCACAAGACA+AGG 0.629304 5_2:-63083083 Msa0780900:CDS
AGTTCCTTTACTGAAAGAAG+GGG 0.634152 5_2:-63082999 Msa0780900:CDS
AGTAATTCAACAGAAAGACA+AGG 0.655740 5_2:-63084849 Msa0780900:five_prime_UTR
GGATGTATATCAGTGTTGGA+CGG 0.669469 5_2:+63083034 None:intergenic
GGAGATGGAGAATCTTCCGA+CGG 0.671736 5_2:+63082869 None:intergenic
TAAATGTGACTCATGGACAA+GGG 0.675226 5_2:-63082913 Msa0780900:CDS
AAAATGTCCCTAATAAAACA+AGG 0.680872 5_2:+63082666 None:intergenic
AAGACAAGGACACAATCCAA+TGG 0.682396 5_2:-63083069 Msa0780900:CDS
TGATATACATCCAGTCACAG+TGG 0.752267 5_2:-63083023 Msa0780900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATAAAATAAATAAA+AGG + chr5_2:63084752-63084771 None:intergenic 0.0%
!! ATTATTATAAAATAAATAAA+AGG + chr5_2:63084752-63084771 None:intergenic 0.0%
!! AATAGTTAATAAGGATAATT+TGG + chr5_2:63083462-63083481 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr5_2:63083461-63083480 Msa0780900:intron 15.0%
!!! TAGTTTTAAAACTAATACTT+TGG - chr5_2:63083485-63083504 Msa0780900:intron 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG - chr5_2:63083588-63083607 Msa0780900:intron 15.0%
!! TATAAAAGTTATGCTTAAAT+TGG - chr5_2:63083735-63083754 Msa0780900:intron 15.0%
!! AAGAAAGGAATATTAAAAAA+AGG + chr5_2:63083974-63083993 None:intergenic 15.0%
!!! TTTTTAATATTCCTTTCTTT+TGG - chr5_2:63083975-63083994 Msa0780900:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr5_2:63083462-63083481 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr5_2:63083461-63083480 Msa0780900:intron 15.0%
!!! TAGTTTTAAAACTAATACTT+TGG - chr5_2:63083485-63083504 Msa0780900:intron 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG - chr5_2:63083588-63083607 Msa0780900:intron 15.0%
!! TATAAAAGTTATGCTTAAAT+TGG - chr5_2:63083735-63083754 Msa0780900:intron 15.0%
!! AAGAAAGGAATATTAAAAAA+AGG + chr5_2:63083974-63083993 None:intergenic 15.0%
!!! TTTTTAATATTCCTTTCTTT+TGG - chr5_2:63083975-63083994 Msa0780900:intron 15.0%
!! AACTGTTAATTATCAGAAAT+GGG - chr5_2:63082838-63082857 Msa0780900:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG + chr5_2:63082895-63082914 None:intergenic 20.0%
!!! TTTTTATGTTTAGACCTTTT+TGG - chr5_2:63082954-63082973 Msa0780900:CDS 20.0%
!! AAAGTGGTATAAAATTAGAT+TGG - chr5_2:63083326-63083345 Msa0780900:intron 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr5_2:63083471-63083490 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr5_2:63083548-63083567 Msa0780900:intron 20.0%
!! AAATAAAAACATAACCACTT+AGG + chr5_2:63083681-63083700 None:intergenic 20.0%
!!! TTTTTAACAAAAGAGACAAA+TGG + chr5_2:63083894-63083913 None:intergenic 20.0%
!!! TTTGTCTCTTTTGTTAAAAA+AGG - chr5_2:63083894-63083913 Msa0780900:intron 20.0%
!! AATTAACAAACCAAACTTTA+GGG + chr5_2:63084035-63084054 None:intergenic 20.0%
!! AAATTAACAAACCAAACTTT+AGG + chr5_2:63084036-63084055 None:intergenic 20.0%
!! AAATTTCACAACATGTTATA+TGG - chr5_2:63084676-63084695 Msa0780900:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG + chr5_2:63084840-63084859 None:intergenic 20.0%
!! AACTGTTAATTATCAGAAAT+GGG - chr5_2:63082838-63082857 Msa0780900:CDS 20.0%
!! AAAAATAAAATCACTTACTG+AGG + chr5_2:63082895-63082914 None:intergenic 20.0%
!!! TTTTTATGTTTAGACCTTTT+TGG - chr5_2:63082954-63082973 Msa0780900:CDS 20.0%
!! AAAGTGGTATAAAATTAGAT+TGG - chr5_2:63083326-63083345 Msa0780900:intron 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr5_2:63083471-63083490 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr5_2:63083548-63083567 Msa0780900:intron 20.0%
!! AAATAAAAACATAACCACTT+AGG + chr5_2:63083681-63083700 None:intergenic 20.0%
!!! TTTTTAACAAAAGAGACAAA+TGG + chr5_2:63083894-63083913 None:intergenic 20.0%
!!! TTTGTCTCTTTTGTTAAAAA+AGG - chr5_2:63083894-63083913 Msa0780900:intron 20.0%
!! AATTAACAAACCAAACTTTA+GGG + chr5_2:63084035-63084054 None:intergenic 20.0%
!! AAATTAACAAACCAAACTTT+AGG + chr5_2:63084036-63084055 None:intergenic 20.0%
!! AAATTTCACAACATGTTATA+TGG - chr5_2:63084676-63084695 Msa0780900:CDS 20.0%
!! TTATACAACAATAACATAGA+TGG + chr5_2:63084840-63084859 None:intergenic 20.0%
! TTCTATGTTCCATTATTCTT+GGG + chr5_2:63082688-63082707 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG - chr5_2:63082746-63082765 Msa0780900:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG + chr5_2:63082839-63082858 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG - chr5_2:63082837-63082856 Msa0780900:CDS 25.0%
! TCATGATATATGTTCCAAAA+AGG + chr5_2:63082971-63082990 None:intergenic 25.0%
! TTGCATTGAATTACTTCTTA+TGG - chr5_2:63083036-63083055 Msa0780900:CDS 25.0%
! CTGATGATTATAATTGATTG+AGG + chr5_2:63083126-63083145 None:intergenic 25.0%
!! TATATGTGTGACTTTTATGA+TGG + chr5_2:63083178-63083197 None:intergenic 25.0%
! GTTTGTAATTGATTGTTTGA+TGG - chr5_2:63083203-63083222 Msa0780900:intron 25.0%
! TTAGAATAAAATGTCAACAC+TGG - chr5_2:63083265-63083284 Msa0780900:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG + chr5_2:63083855-63083874 None:intergenic 25.0%
! GAATATTTGTACCAAAAGAA+AGG + chr5_2:63083989-63084008 None:intergenic 25.0%
! CTAGTATTAACTTCAAAATG+TGG - chr5_2:63084116-63084135 Msa0780900:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG + chr5_2:63084269-63084288 None:intergenic 25.0%
! TTCAAAATAAATGTGACTCA+TGG - chr5_2:63084448-63084467 Msa0780900:intron 25.0%
! TGACAAAGATAAAGATAAAG+AGG - chr5_2:63084621-63084640 Msa0780900:CDS 25.0%
! AAAATGTCCCTAATAAAACA+AGG + chr5_2:63084705-63084724 None:intergenic 25.0%
! TTCTATGTTCCATTATTCTT+GGG + chr5_2:63082688-63082707 None:intergenic 25.0%
!! CTTTTTCTATCATGTTTCTT+AGG - chr5_2:63082746-63082765 Msa0780900:CDS 25.0%
! CATTTCTGATAATTAACAGT+TGG + chr5_2:63082839-63082858 None:intergenic 25.0%
! CAACTGTTAATTATCAGAAA+TGG - chr5_2:63082837-63082856 Msa0780900:CDS 25.0%
! TCATGATATATGTTCCAAAA+AGG + chr5_2:63082971-63082990 None:intergenic 25.0%
! TTGCATTGAATTACTTCTTA+TGG - chr5_2:63083036-63083055 Msa0780900:CDS 25.0%
! CTGATGATTATAATTGATTG+AGG + chr5_2:63083126-63083145 None:intergenic 25.0%
!! TATATGTGTGACTTTTATGA+TGG + chr5_2:63083178-63083197 None:intergenic 25.0%
! GTTTGTAATTGATTGTTTGA+TGG - chr5_2:63083203-63083222 Msa0780900:intron 25.0%
! TTAGAATAAAATGTCAACAC+TGG - chr5_2:63083265-63083284 Msa0780900:intron 25.0%
!! TGTTAATCTGTTTTGCTAAA+TGG + chr5_2:63083855-63083874 None:intergenic 25.0%
! GAATATTTGTACCAAAAGAA+AGG + chr5_2:63083989-63084008 None:intergenic 25.0%
! CTAGTATTAACTTCAAAATG+TGG - chr5_2:63084116-63084135 Msa0780900:intron 25.0%
! ATAATCACATTGTTTGTATG+TGG + chr5_2:63084269-63084288 None:intergenic 25.0%
! TTCAAAATAAATGTGACTCA+TGG - chr5_2:63084448-63084467 Msa0780900:intron 25.0%
! TGACAAAGATAAAGATAAAG+AGG - chr5_2:63084621-63084640 Msa0780900:CDS 25.0%
! AAAATGTCCCTAATAAAACA+AGG + chr5_2:63084705-63084724 None:intergenic 25.0%
AAACCGACATGAAATGAAAT+AGG - chr5_2:63082481-63082500 Msa0780900:three_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG - chr5_2:63082482-63082501 Msa0780900:three_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG - chr5_2:63082519-63082538 Msa0780900:three_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+TGG + chr5_2:63082619-63082638 None:intergenic 30.0%
CTTCTATGTTCCATTATTCT+TGG + chr5_2:63082689-63082708 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG - chr5_2:63082778-63082797 Msa0780900:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG - chr5_2:63082779-63082798 Msa0780900:CDS 30.0%
AGATAGAGATCATGTACTAT+AGG + chr5_2:63083005-63083024 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG + chr5_2:63083098-63083117 None:intergenic 30.0%
TCACAAGTTGTTTATGAAAG+TGG - chr5_2:63083310-63083329 Msa0780900:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG + chr5_2:63083379-63083398 None:intergenic 30.0%
!! TATGTTTTTATTTCCCCACT+TGG - chr5_2:63083688-63083707 Msa0780900:intron 30.0%
!! ATGTTTTTATTTCCCCACTT+GGG - chr5_2:63083689-63083708 Msa0780900:intron 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG - chr5_2:63083751-63083770 Msa0780900:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG - chr5_2:63083752-63083771 Msa0780900:intron 30.0%
!! ACCTATTTTGGGAATAATGT+GGG + chr5_2:63083810-63083829 None:intergenic 30.0%
TACTCAAAATGCCAAAATGA+CGG - chr5_2:63083940-63083959 Msa0780900:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG - chr5_2:63084006-63084025 Msa0780900:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG - chr5_2:63084081-63084100 Msa0780900:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG + chr5_2:63084240-63084259 None:intergenic 30.0%
TTCTTCTCTAGTGATTATGA+TGG - chr5_2:63084400-63084419 Msa0780900:intron 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG - chr5_2:63084694-63084713 Msa0780900:exon 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG - chr5_2:63084695-63084714 Msa0780900:exon 30.0%
AAACCGACATGAAATGAAAT+AGG - chr5_2:63082481-63082500 Msa0780900:three_prime_UTR 30.0%
AACCGACATGAAATGAAATA+GGG - chr5_2:63082482-63082501 Msa0780900:three_prime_UTR 30.0%
AGTAATTCAACAGAAAGACA+AGG - chr5_2:63082519-63082538 Msa0780900:three_prime_UTR 30.0%
!!! TAAGTACTAGCTTTTATGAC+TGG + chr5_2:63082619-63082638 None:intergenic 30.0%
CTTCTATGTTCCATTATTCT+TGG + chr5_2:63082689-63082708 None:intergenic 30.0%
AGCTTACAAAAATTACACAG+TGG - chr5_2:63082778-63082797 Msa0780900:CDS 30.0%
GCTTACAAAAATTACACAGT+GGG - chr5_2:63082779-63082798 Msa0780900:CDS 30.0%
AGATAGAGATCATGTACTAT+AGG + chr5_2:63083005-63083024 None:intergenic 30.0%
ACACACAATTGAGTGTAAAT+TGG + chr5_2:63083098-63083117 None:intergenic 30.0%
TCACAAGTTGTTTATGAAAG+TGG - chr5_2:63083310-63083329 Msa0780900:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG + chr5_2:63083379-63083398 None:intergenic 30.0%
!! TATGTTTTTATTTCCCCACT+TGG - chr5_2:63083688-63083707 Msa0780900:intron 30.0%
!! ATGTTTTTATTTCCCCACTT+GGG - chr5_2:63083689-63083708 Msa0780900:intron 30.0%
!! AAATTGGAAGCACTAAGAAA+AGG - chr5_2:63083751-63083770 Msa0780900:intron 30.0%
!! AATTGGAAGCACTAAGAAAA+GGG - chr5_2:63083752-63083771 Msa0780900:intron 30.0%
!! ACCTATTTTGGGAATAATGT+GGG + chr5_2:63083810-63083829 None:intergenic 30.0%
TACTCAAAATGCCAAAATGA+CGG - chr5_2:63083940-63083959 Msa0780900:intron 30.0%
! TTCTCTTTTGATACTATGAG+TGG - chr5_2:63084006-63084025 Msa0780900:intron 30.0%
TGTATTCTTAGTGTGTCTTT+AGG - chr5_2:63084081-63084100 Msa0780900:intron 30.0%
TATATGTTTGAACAAGAGAG+TGG + chr5_2:63084240-63084259 None:intergenic 30.0%
TTCTTCTCTAGTGATTATGA+TGG - chr5_2:63084400-63084419 Msa0780900:intron 30.0%
!!! TATGGATGCCTTGTTTTATT+AGG - chr5_2:63084694-63084713 Msa0780900:exon 30.0%
!!! ATGGATGCCTTGTTTTATTA+GGG - chr5_2:63084695-63084714 Msa0780900:exon 30.0%
CTCCCTATTTCATTTCATGT+CGG + chr5_2:63082487-63082506 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG - chr5_2:63082555-63082574 Msa0780900:three_prime_UTR 35.0%
AGTGGATGAGAATGTAATGT+AGG + chr5_2:63082593-63082612 None:intergenic 35.0%
TCATGTTTCTTAGGTTCTGT+TGG - chr5_2:63082755-63082774 Msa0780900:CDS 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - chr5_2:63083510-63083529 Msa0780900:intron 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - chr5_2:63083514-63083533 Msa0780900:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr5_2:63083578-63083597 Msa0780900:intron 35.0%
ACCCACATTATTCCCAAAAT+AGG - chr5_2:63083806-63083825 Msa0780900:intron 35.0%
!! GACCTATTTTGGGAATAATG+TGG + chr5_2:63083811-63083830 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG + chr5_2:63083833-63083852 None:intergenic 35.0%
! TTATGATGATGCACAAGACA+AGG - chr5_2:63084285-63084304 Msa0780900:intron 35.0%
CAGTTCCTTTACTGAAAGAA+GGG - chr5_2:63084368-63084387 Msa0780900:intron 35.0%
AGTTCCTTTACTGAAAGAAG+GGG - chr5_2:63084369-63084388 Msa0780900:intron 35.0%
GATGGTGAACAATGCAAAAA+TGG - chr5_2:63084418-63084437 Msa0780900:intron 35.0%
ATAAATGTGACTCATGGACA+AGG - chr5_2:63084454-63084473 Msa0780900:intron 35.0%
TAAATGTGACTCATGGACAA+GGG - chr5_2:63084455-63084474 Msa0780900:intron 35.0%
TTGGAAGGAATATTTGTGTC+TGG + chr5_2:63084559-63084578 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG + chr5_2:63084574-63084593 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG - chr5_2:63084573-63084592 Msa0780900:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG + chr5_2:63084578-63084597 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG + chr5_2:63084650-63084669 None:intergenic 35.0%
CTCCCTATTTCATTTCATGT+CGG + chr5_2:63082487-63082506 None:intergenic 35.0%
AAATCACATGTAACCATAGC+TGG - chr5_2:63082555-63082574 Msa0780900:three_prime_UTR 35.0%
AGTGGATGAGAATGTAATGT+AGG + chr5_2:63082593-63082612 None:intergenic 35.0%
TCATGTTTCTTAGGTTCTGT+TGG - chr5_2:63082755-63082774 Msa0780900:CDS 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - chr5_2:63083510-63083529 Msa0780900:intron 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - chr5_2:63083514-63083533 Msa0780900:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr5_2:63083578-63083597 Msa0780900:intron 35.0%
ACCCACATTATTCCCAAAAT+AGG - chr5_2:63083806-63083825 Msa0780900:intron 35.0%
!! GACCTATTTTGGGAATAATG+TGG + chr5_2:63083811-63083830 None:intergenic 35.0%
GAAGAGTGAATACTTGAGTT+GGG + chr5_2:63083833-63083852 None:intergenic 35.0%
! TTATGATGATGCACAAGACA+AGG - chr5_2:63084285-63084304 Msa0780900:intron 35.0%
CAGTTCCTTTACTGAAAGAA+GGG - chr5_2:63084368-63084387 Msa0780900:intron 35.0%
AGTTCCTTTACTGAAAGAAG+GGG - chr5_2:63084369-63084388 Msa0780900:intron 35.0%
GATGGTGAACAATGCAAAAA+TGG - chr5_2:63084418-63084437 Msa0780900:intron 35.0%
ATAAATGTGACTCATGGACA+AGG - chr5_2:63084454-63084473 Msa0780900:intron 35.0%
TAAATGTGACTCATGGACAA+GGG - chr5_2:63084455-63084474 Msa0780900:intron 35.0%
TTGGAAGGAATATTTGTGTC+TGG + chr5_2:63084559-63084578 None:intergenic 35.0%
TTAGGATGGTTGAAATTGGA+AGG + chr5_2:63084574-63084593 None:intergenic 35.0%
TTCCAATTTCAACCATCCTA+AGG - chr5_2:63084573-63084592 Msa0780900:CDS 35.0%
TTCCTTAGGATGGTTGAAAT+TGG + chr5_2:63084578-63084597 None:intergenic 35.0%
CTTTAACATCGAAACAGAAC+TGG + chr5_2:63084650-63084669 None:intergenic 35.0%
TGAATAATGGCAACTAGCAG+AGG + chr5_2:63082654-63082673 None:intergenic 40.0%
GGTGAGCGAAATGTGAATAA+TGG + chr5_2:63082667-63082686 None:intergenic 40.0%
TTTCGCTCACCCAAGAATAA+TGG - chr5_2:63082676-63082695 Msa0780900:CDS 40.0%
TACACAGTGGGAGATTCTTT+AGG - chr5_2:63082791-63082810 Msa0780900:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG - chr5_2:63082862-63082881 Msa0780900:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG - chr5_2:63082863-63082882 Msa0780900:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG + chr5_2:63082867-63082886 None:intergenic 40.0%
AATGTCAACACTGGCAATAG+TGG - chr5_2:63083274-63083293 Msa0780900:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr5_2:63083618-63083637 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - chr5_2:63083513-63083532 Msa0780900:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr5_2:63083618-63083637 Msa0780900:intron 40.0%
ACTATAGTGTCACACCTAAG+TGG - chr5_2:63083664-63083683 Msa0780900:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG - chr5_2:63083772-63083791 Msa0780900:intron 40.0%
!!! CTTGAGTTGGGACCTATTTT+GGG + chr5_2:63083821-63083840 None:intergenic 40.0%
!!! ACTTGAGTTGGGACCTATTT+TGG + chr5_2:63083822-63083841 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG + chr5_2:63083834-63083853 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG - chr5_2:63084299-63084318 Msa0780900:intron 40.0%
! GGATGTATATCAGTGTTGGA+CGG + chr5_2:63084337-63084356 None:intergenic 40.0%
GACTGGATGTATATCAGTGT+TGG + chr5_2:63084341-63084360 None:intergenic 40.0%
TGATATACATCCAGTCACAG+TGG - chr5_2:63084345-63084364 Msa0780900:intron 40.0%
GCAGTTCCTTTACTGAAAGA+AGG - chr5_2:63084367-63084386 Msa0780900:intron 40.0%
!! GACGGTTTTTGAAGACTCTT+CGG + chr5_2:63084484-63084503 None:intergenic 40.0%
GAAGAGTCTTCAAAAACCGT+CGG - chr5_2:63084483-63084502 Msa0780900:intron 40.0%
TCATCGTCACTATCTTCCTT+AGG + chr5_2:63084592-63084611 None:intergenic 40.0%
TGAATAATGGCAACTAGCAG+AGG + chr5_2:63082654-63082673 None:intergenic 40.0%
GGTGAGCGAAATGTGAATAA+TGG + chr5_2:63082667-63082686 None:intergenic 40.0%
TTTCGCTCACCCAAGAATAA+TGG - chr5_2:63082676-63082695 Msa0780900:CDS 40.0%
TACACAGTGGGAGATTCTTT+AGG - chr5_2:63082791-63082810 Msa0780900:CDS 40.0%
TGCCAAGAAAGAATTCAGCT+TGG - chr5_2:63082862-63082881 Msa0780900:CDS 40.0%
GCCAAGAAAGAATTCAGCTT+GGG - chr5_2:63082863-63082882 Msa0780900:CDS 40.0%
TCCCAAGCTGAATTCTTTCT+TGG + chr5_2:63082867-63082886 None:intergenic 40.0%
AATGTCAACACTGGCAATAG+TGG - chr5_2:63083274-63083293 Msa0780900:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr5_2:63083618-63083637 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - chr5_2:63083513-63083532 Msa0780900:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr5_2:63083618-63083637 Msa0780900:intron 40.0%
ACTATAGTGTCACACCTAAG+TGG - chr5_2:63083664-63083683 Msa0780900:intron 40.0%
GGGATGTACTATTCTTGTTC+TGG - chr5_2:63083772-63083791 Msa0780900:intron 40.0%
!!! CTTGAGTTGGGACCTATTTT+GGG + chr5_2:63083821-63083840 None:intergenic 40.0%
!!! ACTTGAGTTGGGACCTATTT+TGG + chr5_2:63083822-63083841 None:intergenic 40.0%
GGAAGAGTGAATACTTGAGT+TGG + chr5_2:63083834-63083853 None:intergenic 40.0%
AAGACAAGGACACAATCCAA+TGG - chr5_2:63084299-63084318 Msa0780900:intron 40.0%
! GGATGTATATCAGTGTTGGA+CGG + chr5_2:63084337-63084356 None:intergenic 40.0%
GACTGGATGTATATCAGTGT+TGG + chr5_2:63084341-63084360 None:intergenic 40.0%
TGATATACATCCAGTCACAG+TGG - chr5_2:63084345-63084364 Msa0780900:intron 40.0%
GCAGTTCCTTTACTGAAAGA+AGG - chr5_2:63084367-63084386 Msa0780900:intron 40.0%
!! GACGGTTTTTGAAGACTCTT+CGG + chr5_2:63084484-63084503 None:intergenic 40.0%
GAAGAGTCTTCAAAAACCGT+CGG - chr5_2:63084483-63084502 Msa0780900:intron 40.0%
TCATCGTCACTATCTTCCTT+AGG + chr5_2:63084592-63084611 None:intergenic 40.0%
GATTCATATGCTGCCAGCTA+TGG + chr5_2:63082571-63082590 None:intergenic 45.0%
!! AGCTTTTATGACTGGCTCAG+TGG + chr5_2:63082611-63082630 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr5_2:63083617-63083636 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr5_2:63083617-63083636 Msa0780900:intron 45.0%
AATGCCAAAATGACGGTTGC+TGG - chr5_2:63083947-63083966 Msa0780900:intron 45.0%
TGAGTGGCTACCCTAAAGTT+TGG - chr5_2:63084022-63084041 Msa0780900:intron 45.0%
ACGGATCAACTGATGACCAT+TGG + chr5_2:63084318-63084337 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG + chr5_2:63084376-63084395 None:intergenic 45.0%
GACTCATCGTCACCAGAAAT+CGG + chr5_2:63084532-63084551 None:intergenic 45.0%
CGTCACTATCTTCCTTAGGA+TGG + chr5_2:63084588-63084607 None:intergenic 45.0%
TTGACGTGAGATTGTGAGCT+AGG - chr5_2:63084809-63084828 Msa0780900:five_prime_UTR 45.0%
TGACGTGAGATTGTGAGCTA+GGG - chr5_2:63084810-63084829 Msa0780900:five_prime_UTR 45.0%
GATTCATATGCTGCCAGCTA+TGG + chr5_2:63082571-63082590 None:intergenic 45.0%
!! AGCTTTTATGACTGGCTCAG+TGG + chr5_2:63082611-63082630 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr5_2:63083617-63083636 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr5_2:63083617-63083636 Msa0780900:intron 45.0%
AATGCCAAAATGACGGTTGC+TGG - chr5_2:63083947-63083966 Msa0780900:intron 45.0%
TGAGTGGCTACCCTAAAGTT+TGG - chr5_2:63084022-63084041 Msa0780900:intron 45.0%
ACGGATCAACTGATGACCAT+TGG + chr5_2:63084318-63084337 None:intergenic 45.0%
GTTGCCCCTTCTTTCAGTAA+AGG + chr5_2:63084376-63084395 None:intergenic 45.0%
GACTCATCGTCACCAGAAAT+CGG + chr5_2:63084532-63084551 None:intergenic 45.0%
CGTCACTATCTTCCTTAGGA+TGG + chr5_2:63084588-63084607 None:intergenic 45.0%
TTGACGTGAGATTGTGAGCT+AGG - chr5_2:63084809-63084828 Msa0780900:five_prime_UTR 45.0%
TGACGTGAGATTGTGAGCTA+GGG - chr5_2:63084810-63084829 Msa0780900:five_prime_UTR 45.0%
CAGTGGGAGATTCTTTAGGC+TGG - chr5_2:63082795-63082814 Msa0780900:CDS 50.0%
ATGCCACACATAGCCCAAGT+GGG + chr5_2:63083705-63083724 None:intergenic 50.0%
AATGCCACACATAGCCCAAG+TGG + chr5_2:63083706-63083725 None:intergenic 50.0%
! AGGTCCAGCAACCGTCATTT+TGG + chr5_2:63083954-63083973 None:intergenic 50.0%
AAAGGAACTGCCACTGTGAC+TGG + chr5_2:63084358-63084377 None:intergenic 50.0%
GGAGATGGAGAATCTTCCGA+CGG + chr5_2:63084502-63084521 None:intergenic 50.0%
GTCGGAAGATTCTCCATCTC+CGG - chr5_2:63084501-63084520 Msa0780900:CDS 50.0%
!! TCACCAGAAATCGGACTAGC+CGG + chr5_2:63084523-63084542 None:intergenic 50.0%
CAGTGGGAGATTCTTTAGGC+TGG - chr5_2:63082795-63082814 Msa0780900:CDS 50.0%
ATGCCACACATAGCCCAAGT+GGG + chr5_2:63083705-63083724 None:intergenic 50.0%
AATGCCACACATAGCCCAAG+TGG + chr5_2:63083706-63083725 None:intergenic 50.0%
! AGGTCCAGCAACCGTCATTT+TGG + chr5_2:63083954-63083973 None:intergenic 50.0%
AAAGGAACTGCCACTGTGAC+TGG + chr5_2:63084358-63084377 None:intergenic 50.0%
GGAGATGGAGAATCTTCCGA+CGG + chr5_2:63084502-63084521 None:intergenic 50.0%
GTCGGAAGATTCTCCATCTC+CGG - chr5_2:63084501-63084520 Msa0780900:CDS 50.0%
!! TCACCAGAAATCGGACTAGC+CGG + chr5_2:63084523-63084542 None:intergenic 50.0%
TTCCCCACTTGGGCTATGTG+TGG - chr5_2:63083699-63083718 Msa0780900:intron 55.0%
TGCCACACATAGCCCAAGTG+GGG + chr5_2:63083704-63083723 None:intergenic 55.0%
!! GAAATCGGACTAGCCGGAGA+TGG + chr5_2:63084517-63084536 None:intergenic 55.0%
! TCTCCGGCTAGTCCGATTTC+TGG - chr5_2:63084517-63084536 Msa0780900:CDS 55.0%
TTCCCCACTTGGGCTATGTG+TGG - chr5_2:63083699-63083718 Msa0780900:intron 55.0%
TGCCACACATAGCCCAAGTG+GGG + chr5_2:63083704-63083723 None:intergenic 55.0%
!! GAAATCGGACTAGCCGGAGA+TGG + chr5_2:63084517-63084536 None:intergenic 55.0%
! TCTCCGGCTAGTCCGATTTC+TGG - chr5_2:63084517-63084536 Msa0780900:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 63082478 63084912 63082478 ID=Msa0780900;Name=Msa0780900
chr5_2 mRNA 63082478 63084912 63082478 ID=Msa0780900-mRNA-1;Parent=Msa0780900;Name=Msa0780900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=217|1|1|1|1|1|2|177|239
chr5_2 exon 63082478 63083172 63082478 ID=Msa0780900-mRNA-1:exon:18676;Parent=Msa0780900-mRNA-1
chr5_2 exon 63084494 63084912 63084494 ID=Msa0780900-mRNA-1:exon:18675;Parent=Msa0780900-mRNA-1
chr5_2 five_prime_UTR 63084696 63084912 63084696 ID=Msa0780900-mRNA-1:five_prime_utr;Parent=Msa0780900-mRNA-1
chr5_2 CDS 63084494 63084695 63084494 ID=Msa0780900-mRNA-1:cds;Parent=Msa0780900-mRNA-1
chr5_2 CDS 63082655 63083172 63082655 ID=Msa0780900-mRNA-1:cds;Parent=Msa0780900-mRNA-1
chr5_2 three_prime_UTR 63082478 63082654 63082478 ID=Msa0780900-mRNA-1:three_prime_utr;Parent=Msa0780900-mRNA-1
Gene Sequence

>Msa0780900

ATGGAACATAGAAGAAACTCATCATTGTTTCTATTCTCAACTCTTTCTTGTCTTTTTCTATCATGTTTCTTAGGTTCTGTTGGAGCTTACAAAAATTACACAGTGGGAGATTCTTTAGGCTGGTTTGACAATCTTGAAAAGCCAACTGTTAATTATCAGAAATGGGTTGCCAAGAAAGAATTCAGCTTGGGAGATTTCCTCATTTTCAACACAGACAATAACCACTCTCTTGTTCAAACATATAATTTCACCACATACAAACAATGTGATTATGATGATGCACAAGACAAGGACACAATCCAATGGTCATCAGTTGATCCGTCCAACACTGATATACATCCAGTCACAGTGGCAGTTCCTTTACTGAAAGAAGGGGCAACATATTTCTTCTCTAGTGATTATGATGGTGAACAATGCAAAAATGGACAACATTTCAAAATAAATGTGACTCATGGACAAGGGTTGCCGAAGAGTCTTCAAAAACCGTCGGAAGATTCTCCATCTCCGGCTAGTCCGATTTCTGGTGACGATGAGTCTGCACCAGACACAAATATTCCTTCCAATTTCAACCATCCTAAGGAAGATAGTGACGATGAAAAAACTAGTGACAAAGATAAAGATAAAGAGGAATCCAGTTCTGTTTCGATGTTAAAGTATGCAAAATTTCACAACATGTTATATGGATGCCTTGTTTTATTAGGGACATTTTTCTTTTTTTAG
Protein sequence

>Msa0780900

MEHRRNSSLFLFSTLSCLFLSCFLGSVGAYKNYTVGDSLGWFDNLEKPTVNYQKWVAKKEFSLGDFLIFNTDNNHSLVQTYNFTTYKQCDYDDAQDKDTIQWSSVDPSNTDIHPVTVAVPLLKEGATYFFSSDYDGEQCKNGQHFKINVTHGQGLPKSLQKPSEDSPSPASPISGDDESAPDTNIPSNFNHPKEDSDDEKTSDKDKDKEESSSVSMLKYAKFHNMLYGCLVLLGTFFFF*