Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0822770 | XP_003617415.1 | 94.186 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 6.89e-52 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0822770 | G7KD46 | 94.186 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 3.29e-52 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0784860 | Msa0822770 | 0.839593 | 1.444396e-57 | -8.615850e-47 |
| Msa0813720 | Msa0822770 | 0.800072 | 1.711125e-48 | -8.615850e-47 |
| Msa0822770 | Msa0933290 | 0.804348 | 2.255644e-49 | -8.615850e-47 |
| Msa0822770 | Msa1465960 | 0.823845 | 1.112251e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0822770 | MtrunA17_Chr5g0443071 | 94.186 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 6.34e-56 | 167 |
| Msa0822770 | MtrunA17_Chr4g0052071 | 50.000 | 74 | 30 | 3 | 17 | 86 | 3 | 73 | 8.91e-17 | 67.8 |
| Msa0822770 | MtrunA17_Chr4g0052051 | 48.000 | 75 | 31 | 3 | 17 | 86 | 3 | 74 | 1.45e-15 | 64.7 |
| Msa0822770 | MtrunA17_Chr3g0096231 | 56.250 | 64 | 26 | 2 | 21 | 83 | 3 | 65 | 2.30e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0822770 | AT5G46295.1 | 60.465 | 43 | 16 | 1 | 44 | 86 | 30 | 71 | 3.15e-13 | 59.3 |
| Msa0822770 | AT1G06475.1 | 56.818 | 44 | 18 | 1 | 43 | 85 | 49 | 92 | 2.77e-12 | 57.4 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGAGATTCTCTGCGTTT+AGG | 0.350104 | 5_3:-61149301 | Msa0822770:CDS |
| TCGTGGATACTGTCGTGTTT+AGG | 0.373591 | 5_3:-61149247 | Msa0822770:CDS |
| AGAAAGTGAAGAAAGAAGTT+TGG | 0.381061 | 5_3:-61149325 | Msa0822770:CDS |
| TAGTTTGAGAAAGCGTTTGA+TGG | 0.413544 | 5_3:-61149375 | Msa0822770:CDS |
| GAAAGAAGAGTAGCATGTTA+TGG | 0.462290 | 5_3:-61149352 | Msa0822770:CDS |
| TGCTTCTTCTATCTTTGTTG+TGG | 0.470772 | 5_3:-61149182 | Msa0822770:CDS |
| TCATCTTCAAATGACATTCG+TGG | 0.492578 | 5_3:-61149264 | Msa0822770:CDS |
| AGTTTGAGAAAGCGTTTGAT+GGG | 0.536928 | 5_3:-61149374 | Msa0822770:CDS |
| GGAGATTCTCTGCGTTTAGG+TGG | 0.556332 | 5_3:-61149298 | Msa0822770:CDS |
| TGAAGAAAGAAGTTTGGCCA+TGG | 0.569626 | 5_3:-61149319 | Msa0822770:CDS |
| AAGATAGAAGAAGCAAACAG+TGG | 0.638854 | 5_3:+61149190 | None:intergenic |
| CTAAACGCAGAGAATCTCCA+TGG | 0.659319 | 5_3:+61149302 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTGTAATATCTTTTGTT+TGG | + | chr5_3:61149180-61149199 | None:intergenic | 20.0% |
| AGAAAGTGAAGAAAGAAGTT+TGG | - | chr5_3:61149251-61149270 | Msa0822770:CDS | 30.0% | |
| !! | TAGTTTGAGAAAGCGTTTGA+TGG | - | chr5_3:61149201-61149220 | Msa0822770:CDS | 35.0% |
| !! | AGTTTGAGAAAGCGTTTGAT+GGG | - | chr5_3:61149202-61149221 | Msa0822770:CDS | 35.0% |
| GAAAGAAGAGTAGCATGTTA+TGG | - | chr5_3:61149224-61149243 | Msa0822770:CDS | 35.0% | |
| TCATCTTCAAATGACATTCG+TGG | - | chr5_3:61149312-61149331 | Msa0822770:CDS | 35.0% | |
| AAGATAGAAGAAGCAAACAG+TGG | + | chr5_3:61149389-61149408 | None:intergenic | 35.0% | |
| TGCTTCTTCTATCTTTGTTG+TGG | - | chr5_3:61149394-61149413 | Msa0822770:CDS | 35.0% | |
| TGAAGAAAGAAGTTTGGCCA+TGG | - | chr5_3:61149257-61149276 | Msa0822770:CDS | 40.0% | |
| CTAAACGCAGAGAATCTCCA+TGG | + | chr5_3:61149277-61149296 | None:intergenic | 45.0% | |
| CATGGAGATTCTCTGCGTTT+AGG | - | chr5_3:61149275-61149294 | Msa0822770:CDS | 45.0% | |
| TCGTGGATACTGTCGTGTTT+AGG | - | chr5_3:61149329-61149348 | Msa0822770:CDS | 45.0% | |
| GGAGATTCTCTGCGTTTAGG+TGG | - | chr5_3:61149278-61149297 | Msa0822770:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 61149169 | 61149429 | 61149169 | ID=Msa0822770;Name=Msa0822770 |
| chr5_3 | mRNA | 61149169 | 61149429 | 61149169 | ID=Msa0822770-mRNA-1;Parent=Msa0822770;Name=Msa0822770-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|86 |
| chr5_3 | exon | 61149169 | 61149429 | 61149169 | ID=Msa0822770-mRNA-1:exon:18845;Parent=Msa0822770-mRNA-1 |
| chr5_3 | CDS | 61149169 | 61149429 | 61149169 | ID=Msa0822770-mRNA-1:cds;Parent=Msa0822770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
