AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0823100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823100 MtrunA17_Chr5g0443381 84.384 333 36 4 1 319 1 331 0.0 532
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 89 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATAATAATGAAGGATTTA+AGG 0.221973 5_3:-61719025 Msa0823100:CDS
ACAATATGAAAACTGAAATT+TGG 0.241629 5_3:+61717145 None:intergenic
ATCAATATCAACAAAATCTT+TGG 0.250643 5_3:+61719257 None:intergenic
AGCAAGTTGACTGCTTTAAT+AGG 0.258946 5_3:+61717268 None:intergenic
CATGCAACTGAAATGAGTTT+TGG 0.306374 5_3:+61717093 None:intergenic
TAAAAGAACAGGCCTCAAAA+AGG 0.317516 5_3:+61717225 None:intergenic
ATCTGAAAATCATAGCAAAA+TGG 0.345325 5_3:-61719412 Msa0823100:CDS
TACAGCTTTACTGATTGATT+TGG 0.357160 5_3:-61717194 Msa0823100:three_prime_UTR
TGACTCCTTAGCACTTTCAT+AGG 0.369555 5_3:+61718774 None:intergenic
GTGGATAAATAAACAAATAA+AGG 0.379814 5_3:+61719839 None:intergenic
GGCCTCAGAAGCTGTAGAAT+TGG 0.384608 5_3:-61719130 Msa0823100:CDS
GCAAGTTGACTGCTTTAATA+GGG 0.387804 5_3:+61717269 None:intergenic
GTTAACTTCTATTGCATCAC+TGG 0.396043 5_3:+61719170 None:intergenic
CCACCTCATCATTATCTTCA+AGG 0.400815 5_3:+61719073 None:intergenic
AATGACTCCTCAATTCCCTT+TGG 0.431098 5_3:+61718853 None:intergenic
CTCAAAGGCAACACACCTCT+TGG 0.437333 5_3:-61718491 Msa0823100:CDS
AGGCGCTGCAGTTCTCATGC+TGG 0.451592 5_3:-61717299 Msa0823100:three_prime_UTR
GGCGCTGCAGTTCTCATGCT+GGG 0.451632 5_3:-61717298 Msa0823100:three_prime_UTR
CCTCGATATACCTTTGCTCA+TGG 0.456597 5_3:+61717319 None:intergenic
AGATTCATTAAGTGAAGATA+AGG 0.458262 5_3:-61719343 Msa0823100:CDS
GTTGGGCAGAGTCAAAGGTT+TGG 0.462169 5_3:+61719730 None:intergenic
AGGCCATCTTATTCTCAACT+TGG 0.469285 5_3:+61719148 None:intergenic
GTAAGAAAACGTAAAAGAAC+AGG 0.473028 5_3:+61717214 None:intergenic
AAAGGGAATTGAGGAGTCAT+TGG 0.475443 5_3:-61718851 Msa0823100:CDS
TGAAGTGGTTGAAACAGTTA+AGG 0.481899 5_3:-61718998 Msa0823100:CDS
CTGCAGTTCTCATGCTGGGA+GGG 0.483088 5_3:-61717294 Msa0823100:three_prime_UTR
AATGAAACCACATCAGTTGA+AGG 0.488510 5_3:+61718943 None:intergenic
TATGAAAACTGAAATTTGGT+AGG 0.490910 5_3:+61717149 None:intergenic
CTCGATATACCTTTGCTCAT+GGG 0.491245 5_3:+61717320 None:intergenic
CTCTTTGAAATTCTGAGGCA+TGG 0.493717 5_3:-61718453 Msa0823100:CDS
TCTAGAGGCATTGAAAATCA+AGG 0.497245 5_3:-61718682 Msa0823100:CDS
AACATTGCATGAAACTCAAA+AGG 0.501815 5_3:-61719373 Msa0823100:CDS
GACTCCTCAATTCCCTTTGG+AGG 0.504945 5_3:+61718856 None:intergenic
TGTTGGTTGGGCAGAGTCAA+AGG 0.507031 5_3:+61719725 None:intergenic
GTAGGTGAAGAGTGTTGGTT+GGG 0.507556 5_3:+61719713 None:intergenic
AATAAACAAATAAAGGAGAA+TGG 0.511031 5_3:+61719846 None:intergenic
TTGGAATTGAATGATGAGAA+AGG 0.511441 5_3:-61719111 Msa0823100:CDS
TCAAATAGTTGGGAGCTTGA+AGG 0.524609 5_3:-61717350 Msa0823100:intron
TGTTCCTCCAAAGGGAATTG+AGG 0.527183 5_3:-61718860 Msa0823100:CDS
GTTGGGAGCTTGAAGGAGGT+TGG 0.528065 5_3:-61717343 Msa0823100:three_prime_UTR
GCGGACTCTTTGAAATTCTG+AGG 0.535370 5_3:-61718458 Msa0823100:CDS
ACTTCAAAGGATAATAATGA+AGG 0.536408 5_3:-61719033 Msa0823100:CDS
TGAAGATAATGATGAGGTGG+AGG 0.538135 5_3:-61719070 Msa0823100:CDS
CACCTCATCATTATCTTCAA+GGG 0.545624 5_3:+61719074 None:intergenic
TGGGTTTGTAGTTGAACAGT+AGG 0.549277 5_3:+61719695 None:intergenic
TTCATTAAGTGAAGATAAGG+AGG 0.550055 5_3:-61719340 Msa0823100:CDS
TTTGGTATCACTGATAGACT+TGG 0.551465 5_3:+61719275 None:intergenic
AAAATGGAAGGAAATTTGAA+TGG 0.553733 5_3:-61719396 Msa0823100:CDS
GAACAGTAGGTGAAGAGTGT+TGG 0.557856 5_3:+61719708 None:intergenic
AATTGATGATGAGGGTGAAA+AGG 0.558460 5_3:-61718884 Msa0823100:CDS
TAACCAAGTTGAGAATAAGA+TGG 0.563805 5_3:-61719151 Msa0823100:CDS
GCATGGTGATAGATAAATCA+AGG 0.565697 5_3:-61718436 Msa0823100:intron
CTAGAGGCATTGAAAATCAA+GGG 0.566438 5_3:-61718681 Msa0823100:CDS
AGGATTTAAGGTAACTGAAG+TGG 0.567004 5_3:-61719013 Msa0823100:CDS
TTCCAATTCTACAGCTTCTG+AGG 0.567350 5_3:+61719128 None:intergenic
GCTTTGGAGACAAGTGGTGA+GGG 0.567385 5_3:-61718907 Msa0823100:CDS
GGACATGACAGTAGCTGAGG+AGG 0.567686 5_3:-61718977 Msa0823100:CDS
GAAAAGGTTGTTCCTCCAAA+GGG 0.568369 5_3:-61718868 Msa0823100:CDS
TGTTGAGTCATGCAAGGTGG+TGG 0.568757 5_3:-61719229 Msa0823100:CDS
ATGAAAACTGAAATTTGGTA+GGG 0.571179 5_3:+61717150 None:intergenic
TGCTTTGGAGACAAGTGGTG+AGG 0.574628 5_3:-61718908 Msa0823100:CDS
TCTGCTGATGATGATTATGA+TGG 0.574692 5_3:-61719303 Msa0823100:CDS
TGAAAAGGTTGTTCCTCCAA+AGG 0.575350 5_3:-61718869 Msa0823100:CDS
AGTAGGTGAAGAGTGTTGGT+TGG 0.576236 5_3:+61719712 None:intergenic
GTTTGTTACTTCGAATAATG+CGG 0.576414 5_3:+61717064 None:intergenic
ACCTCTTGGAAGAATTGTTG+CGG 0.585031 5_3:-61718477 Msa0823100:CDS
ATCATCCTATGAAAGTGCTA+AGG 0.585651 5_3:-61718779 Msa0823100:CDS
GCTGCAGTTCTCATGCTGGG+AGG 0.586808 5_3:-61717295 Msa0823100:three_prime_UTR
GTATAGGCATGGTTACTGAC+TGG 0.588901 5_3:+61719789 None:intergenic
GAAAATCATAGCAAAATGGA+AGG 0.594613 5_3:-61719408 Msa0823100:CDS
TAAGGACATGACAGTAGCTG+AGG 0.602185 5_3:-61718980 Msa0823100:CDS
AACTGAATTAGCTACTTCAA+AGG 0.610607 5_3:-61719046 Msa0823100:CDS
GGAGGTTGGCCCATGAGCAA+AGG 0.613149 5_3:-61717329 Msa0823100:three_prime_UTR
GAAGTTGCTTTGGAGACAAG+TGG 0.613463 5_3:-61718913 Msa0823100:CDS
CCTTGAAGATAATGATGAGG+TGG 0.613497 5_3:-61719073 Msa0823100:CDS
AATAGTTGGGAGCTTGAAGG+AGG 0.616938 5_3:-61717347 Msa0823100:intron
TGGTGAGGGAATTGATGATG+AGG 0.630447 5_3:-61718893 Msa0823100:CDS
CCATGAGCAAAGGTATATCG+AGG 0.636235 5_3:-61717319 Msa0823100:three_prime_UTR
GGTGAGGGAATTGATGATGA+GGG 0.638068 5_3:-61718892 Msa0823100:CDS
TCCGCAACAATTCTTCCAAG+AGG 0.641763 5_3:+61718476 None:intergenic
ATCTACACAGGAGAAAGTTG+AGG 0.647223 5_3:-61718824 Msa0823100:CDS
GTCATTGGTGACATCTACAC+AGG 0.650440 5_3:-61718836 Msa0823100:CDS
ACTGTTCAACTACAAACCCA+AGG 0.650999 5_3:-61719692 Msa0823100:intron
ATCCCTTGAAGATAATGATG+AGG 0.651217 5_3:-61719076 Msa0823100:CDS
TAGAGGCATTGAAAATCAAG+GGG 0.660240 5_3:-61718680 Msa0823100:intron
TGATTGATTTGGTTTCAAAG+TGG 0.691411 5_3:-61717183 Msa0823100:three_prime_UTR
GACACGATTGAAGAATCTAG+AGG 0.692176 5_3:-61718697 Msa0823100:CDS
TGAACATGTTGAGTCATGCA+AGG 0.711218 5_3:-61719235 Msa0823100:CDS
ACATGTTGAGTCATGCAAGG+TGG 0.794332 5_3:-61719232 Msa0823100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTATAGTAATATGA+GGG + chr5_3:61718553-61718572 None:intergenic 10.0%
!!! ATATTTTTTATAGTAATATG+AGG + chr5_3:61718554-61718573 None:intergenic 10.0%
!!! AATAGTTTTTCTTTTTTATA+TGG - chr5_3:61718695-61718714 Msa0823100:CDS 10.0%
!!! TTTTCTTTCAATAAACAAAT+TGG - chr5_3:61717335-61717354 Msa0823100:three_prime_UTR 15.0%
!!! ACTAACATGTTTTAATTAAA+AGG - chr5_3:61718484-61718503 Msa0823100:CDS 15.0%
!! AAAAGAAAAACTATTTAACA+AGG + chr5_3:61718690-61718709 None:intergenic 15.0%
!! TTATAGTAATCTAAAAATGT+GGG - chr5_3:61718805-61718824 Msa0823100:CDS 15.0%
!! AATCAAAAAAAAATAATCAC+AGG + chr5_3:61719490-61719509 None:intergenic 15.0%
!!! TTTTTTTTGATTCAAATAGT+TGG - chr5_3:61719498-61719517 Msa0823100:intron 15.0%
!!! TTTTTTTGATTCAAATAGTT+GGG - chr5_3:61719499-61719518 Msa0823100:intron 15.0%
!!! TCCTAATTATGATATTTTTA+TGG - chr5_3:61719842-61719861 Msa0823100:five_prime_UTR 15.0%
!! TCCATAAAAATATCATAATT+AGG + chr5_3:61719846-61719865 None:intergenic 15.0%
!! AATAAACAAATAAAGGAGAA+TGG + chr5_3:61717016-61717035 None:intergenic 20.0%
!! GTGGATAAATAAACAAATAA+AGG + chr5_3:61717023-61717042 None:intergenic 20.0%
!!! TGTTTTGTTTTTTTTCTTGA+AGG + chr5_3:61717095-61717114 None:intergenic 20.0%
!! GTAACAATTATTGAAAACAT+AGG + chr5_3:61717285-61717304 None:intergenic 20.0%
!!! TGTTTTTTTTTTTTATCAGC+AGG - chr5_3:61717415-61717434 Msa0823100:intron 20.0%
!! ATCAATATCAACAAAATCTT+TGG + chr5_3:61717605-61717624 None:intergenic 20.0%
!! GAAAATAAAATCCTAAACTA+AGG + chr5_3:61718458-61718477 None:intergenic 20.0%
!! ATTACTATAACAAAGTAACT+AGG + chr5_3:61718795-61718814 None:intergenic 20.0%
!! GTTATAGTAATCTAAAAATG+TGG - chr5_3:61718804-61718823 Msa0823100:CDS 20.0%
!!! ATATTTTCAATAGATTCTCT+TGG - chr5_3:61718858-61718877 Msa0823100:CDS 20.0%
!! ACAATATGAAAACTGAAATT+TGG + chr5_3:61719717-61719736 None:intergenic 20.0%
!!! GTTTTTTTTCTTGAAGGTAT+AGG + chr5_3:61717089-61717108 None:intergenic 25.0%
! AAAACAGAAACAACAAAAAG+AGG + chr5_3:61717228-61717247 None:intergenic 25.0%
! GAAAAACAAAGAAAACATGA+GGG + chr5_3:61717320-61717339 None:intergenic 25.0%
! AGAAAAACAAAGAAAACATG+AGG + chr5_3:61717321-61717340 None:intergenic 25.0%
!! AAATAGATCTTTTCCACATA+CGG - chr5_3:61717366-61717385 Msa0823100:intron 25.0%
! ATCTGAAAATCATAGCAAAA+TGG - chr5_3:61717447-61717466 Msa0823100:intron 25.0%
! AAAATGGAAGGAAATTTGAA+TGG - chr5_3:61717463-61717482 Msa0823100:intron 25.0%
! AGATTCATTAAGTGAAGATA+AGG - chr5_3:61717516-61717535 Msa0823100:intron 25.0%
! ACTTCAAAGGATAATAATGA+AGG - chr5_3:61717826-61717845 Msa0823100:intron 25.0%
! GGATAATAATGAAGGATTTA+AGG - chr5_3:61717834-61717853 Msa0823100:intron 25.0%
! ATTGTTAATCAAACTTTGGT+TGG + chr5_3:61718586-61718605 None:intergenic 25.0%
! ATGCATTGTTAATCAAACTT+TGG + chr5_3:61718590-61718609 None:intergenic 25.0%
!! CAATGCATTTTATGTCAATA+TGG - chr5_3:61718602-61718621 Msa0823100:intron 25.0%
! TTTAACAAGGAATGTTTAAC+TGG + chr5_3:61718677-61718696 None:intergenic 25.0%
!!! AGATTTTTGAACCTCTAAAA+GGG + chr5_3:61718746-61718765 None:intergenic 25.0%
! TAGATATTTGACTAGACTTT+AGG + chr5_3:61718895-61718914 None:intergenic 25.0%
!! AATGATGAGATGTTTAAGTT+TGG + chr5_3:61718995-61719014 None:intergenic 25.0%
! CAAAGTTAACTTAAATCGAT+AGG - chr5_3:61719159-61719178 Msa0823100:CDS 25.0%
! TAATGAAATGTGACAATTCA+TGG + chr5_3:61719267-61719286 None:intergenic 25.0%
!! AATTTAGAGGATGAACTTTT+TGG - chr5_3:61719321-61719340 Msa0823100:CDS 25.0%
! ATGAAAACTGAAATTTGGTA+GGG + chr5_3:61719712-61719731 None:intergenic 25.0%
! TATGAAAACTGAAATTTGGT+AGG + chr5_3:61719713-61719732 None:intergenic 25.0%
!! TTGTTTTGTAAAGAGAATGG+TGG + chr5_3:61717042-61717061 None:intergenic 30.0%
!!! GCTTTGTTTTGTAAAGAGAA+TGG + chr5_3:61717045-61717064 None:intergenic 30.0%
AGAAACAACAAAAAGAGGTT+GGG + chr5_3:61717223-61717242 None:intergenic 30.0%
TAGTTTACTACATCCTTGTA+TGG - chr5_3:61717256-61717275 Msa0823100:three_prime_UTR 30.0%
AAAACATAGGATACCATACA+AGG + chr5_3:61717272-61717291 None:intergenic 30.0%
ATAGAGATATATACCGTATG+TGG + chr5_3:61717382-61717401 None:intergenic 30.0%
ATACGGTATATATCTCTATG+TGG - chr5_3:61717383-61717402 Msa0823100:intron 30.0%
GAAAATCATAGCAAAATGGA+AGG - chr5_3:61717451-61717470 Msa0823100:intron 30.0%
AACATTGCATGAAACTCAAA+AGG - chr5_3:61717486-61717505 Msa0823100:intron 30.0%
TTCATTAAGTGAAGATAAGG+AGG - chr5_3:61717519-61717538 Msa0823100:intron 30.0%
! TAACCAAGTTGAGAATAAGA+TGG - chr5_3:61717708-61717727 Msa0823100:intron 30.0%
TTGGAATTGAATGATGAGAA+AGG - chr5_3:61717748-61717767 Msa0823100:intron 30.0%
AACTGAATTAGCTACTTCAA+AGG - chr5_3:61717813-61717832 Msa0823100:intron 30.0%
! TGATGTGGTTTCATTTTCAA+GGG - chr5_3:61717924-61717943 Msa0823100:intron 30.0%
! AGCACAATCTAAAATGTCTA+TGG + chr5_3:61718264-61718283 None:intergenic 30.0%
AACATGTTGAAACAGATGTA+GGG - chr5_3:61718507-61718526 Msa0823100:CDS 30.0%
GTCAATATGGTCAAAATTGT+TGG - chr5_3:61718615-61718634 Msa0823100:intron 30.0%
! CAGATTTTTGAACCTCTAAA+AGG + chr5_3:61718747-61718766 None:intergenic 30.0%
TTGGTTATTGACCAATCTTT+GGG + chr5_3:61718976-61718995 None:intergenic 30.0%
TTTGGTTATTGACCAATCTT+TGG + chr5_3:61718977-61718996 None:intergenic 30.0%
TCCCTAAAATTGTAAAACAC+CGG - chr5_3:61719075-61719094 Msa0823100:CDS 30.0%
! ACCGGTGTTTTACAATTTTA+GGG + chr5_3:61719079-61719098 None:intergenic 30.0%
! AACCGGTGTTTTACAATTTT+AGG + chr5_3:61719080-61719099 None:intergenic 30.0%
AAACCAAGGATGAATTTAAC+CGG + chr5_3:61719097-61719116 None:intergenic 30.0%
ATTGCTAATTCGTAAAACCA+AGG + chr5_3:61719111-61719130 None:intergenic 30.0%
TGGATTGACGTTAAAATCTT+CGG - chr5_3:61719341-61719360 Msa0823100:CDS 30.0%
! GGCTAATTCGTTAACAATTT+TGG + chr5_3:61719368-61719387 None:intergenic 30.0%
GTAAGAAAACGTAAAAGAAC+AGG + chr5_3:61719648-61719667 None:intergenic 30.0%
! TACAGCTTTACTGATTGATT+TGG - chr5_3:61719665-61719684 Msa0823100:intron 30.0%
!! TGATTGATTTGGTTTCAAAG+TGG - chr5_3:61719676-61719695 Msa0823100:intron 30.0%
TTGTAAACTACAATAAACCG+CGG - chr5_3:61719733-61719752 Msa0823100:CDS 30.0%
! GTTTGTTACTTCGAATAATG+CGG + chr5_3:61719798-61719817 None:intergenic 30.0%
!! TTTTCTTGAAGGTATAGGCA+TGG + chr5_3:61717084-61717103 None:intergenic 35.0%
!!! CAAAGGTTTGGTTTTGTTCT+TGG + chr5_3:61717120-61717139 None:intergenic 35.0%
CAGAAACAACAAAAAGAGGT+TGG + chr5_3:61717224-61717243 None:intergenic 35.0%
AGAAAACATGAGGGTTTGAA+AGG + chr5_3:61717311-61717330 None:intergenic 35.0%
! TCTGCTGATGATGATTATGA+TGG - chr5_3:61717556-61717575 Msa0823100:intron 35.0%
TTTGGTATCACTGATAGACT+TGG + chr5_3:61717587-61717606 None:intergenic 35.0%
GTTAACTTCTATTGCATCAC+TGG + chr5_3:61717692-61717711 None:intergenic 35.0%
ATCCCTTGAAGATAATGATG+AGG - chr5_3:61717783-61717802 Msa0823100:intron 35.0%
CACCTCATCATTATCTTCAA+GGG + chr5_3:61717788-61717807 None:intergenic 35.0%
! AGGATTTAAGGTAACTGAAG+TGG - chr5_3:61717846-61717865 Msa0823100:intron 35.0%
TGAAGTGGTTGAAACAGTTA+AGG - chr5_3:61717861-61717880 Msa0823100:intron 35.0%
AATGAAACCACATCAGTTGA+AGG + chr5_3:61717919-61717938 None:intergenic 35.0%
! CTGATGTGGTTTCATTTTCA+AGG - chr5_3:61717923-61717942 Msa0823100:intron 35.0%
!! ATTTTCAAGGGAAGTTGCTT+TGG - chr5_3:61717936-61717955 Msa0823100:intron 35.0%
!! AATTGATGATGAGGGTGAAA+AGG - chr5_3:61717975-61717994 Msa0823100:intron 35.0%
!! ATCATCCTATGAAAGTGCTA+AGG - chr5_3:61718080-61718099 Msa0823100:intron 35.0%
!! TCTAGAGGCATTGAAAATCA+AGG - chr5_3:61718177-61718196 Msa0823100:intron 35.0%
!! CTAGAGGCATTGAAAATCAA+GGG - chr5_3:61718178-61718197 Msa0823100:intron 35.0%
!! TAGAGGCATTGAAAATCAAG+GGG - chr5_3:61718179-61718198 Msa0823100:intron 35.0%
CTGTCACAAAAGAAAGAAGA+AGG + chr5_3:61718332-61718351 None:intergenic 35.0%
GCATGGTGATAGATAAATCA+AGG - chr5_3:61718423-61718442 Msa0823100:intron 35.0%
GGTGAGTAAAACCTTAGTTT+AGG - chr5_3:61718444-61718463 Msa0823100:CDS 35.0%
GAACATGTTGAAACAGATGT+AGG - chr5_3:61718506-61718525 Msa0823100:CDS 35.0%
AACTTGTGAACCAAACTTGT+TGG - chr5_3:61718942-61718961 Msa0823100:CDS 35.0%
! ACTTGTGAACCAAACTTGTT+GGG - chr5_3:61718943-61718962 Msa0823100:CDS 35.0%
ACAGTCCAATCTTGAACTTA+GGG + chr5_3:61719032-61719051 None:intergenic 35.0%
AACAGTCCAATCTTGAACTT+AGG + chr5_3:61719033-61719052 None:intergenic 35.0%
ACTTAAATCGATAGGCAACA+TGG - chr5_3:61719167-61719186 Msa0823100:CDS 35.0%
CGGCATGTTAAATAAAGATG+TGG - chr5_3:61719192-61719211 Msa0823100:CDS 35.0%
AAACTTGCAACATGTTGTCT+AGG - chr5_3:61719293-61719312 Msa0823100:CDS 35.0%
AACTTGCAACATGTTGTCTA+GGG - chr5_3:61719294-61719313 Msa0823100:CDS 35.0%
CAAGACAGATTAGATTATGC+AGG + chr5_3:61719407-61719426 None:intergenic 35.0%
AGCTAAATTAAGCTCTTGAG+TGG - chr5_3:61719445-61719464 Msa0823100:intron 35.0%
! GCAAGTTGACTGCTTTAATA+GGG + chr5_3:61719593-61719612 None:intergenic 35.0%
! AGCAAGTTGACTGCTTTAAT+AGG + chr5_3:61719594-61719613 None:intergenic 35.0%
! TTGATTTTGAGCCCTTTTTG+AGG - chr5_3:61719622-61719641 Msa0823100:intron 35.0%
AAAAGAACAGGCCTCAAAAA+GGG + chr5_3:61719636-61719655 None:intergenic 35.0%
TAAAAGAACAGGCCTCAAAA+AGG + chr5_3:61719637-61719656 None:intergenic 35.0%
TGTAAACTACAATAAACCGC+GGG - chr5_3:61719734-61719753 Msa0823100:CDS 35.0%
! CATGCAACTGAAATGAGTTT+TGG + chr5_3:61719769-61719788 None:intergenic 35.0%
TGGGTTTGTAGTTGAACAGT+AGG + chr5_3:61717167-61717186 None:intergenic 40.0%
ACTGTTCAACTACAAACCCA+AGG - chr5_3:61717167-61717186 Msa0823100:three_prime_UTR 40.0%
CAACAAAAAGAGGTTGGGAA+AGG + chr5_3:61717218-61717237 None:intergenic 40.0%
TGAACATGTTGAGTCATGCA+AGG - chr5_3:61717624-61717643 Msa0823100:intron 40.0%
AGGCCATCTTATTCTCAACT+TGG + chr5_3:61717714-61717733 None:intergenic 40.0%
TTCCAATTCTACAGCTTCTG+AGG + chr5_3:61717734-61717753 None:intergenic 40.0%
CCACCTCATCATTATCTTCA+AGG + chr5_3:61717789-61717808 None:intergenic 40.0%
CCTTGAAGATAATGATGAGG+TGG - chr5_3:61717786-61717805 Msa0823100:intron 40.0%
TGAAGATAATGATGAGGTGG+AGG - chr5_3:61717789-61717808 Msa0823100:intron 40.0%
! TGTTTTGCCTTCAACTGATG+TGG - chr5_3:61717909-61717928 Msa0823100:intron 40.0%
TGAAAAGGTTGTTCCTCCAA+AGG - chr5_3:61717990-61718009 Msa0823100:intron 40.0%
GAAAAGGTTGTTCCTCCAAA+GGG - chr5_3:61717991-61718010 Msa0823100:intron 40.0%
AATGACTCCTCAATTCCCTT+TGG + chr5_3:61718009-61718028 None:intergenic 40.0%
AAAGGGAATTGAGGAGTCAT+TGG - chr5_3:61718008-61718027 Msa0823100:intron 40.0%
ATCTACACAGGAGAAAGTTG+AGG - chr5_3:61718035-61718054 Msa0823100:intron 40.0%
TGACTCCTTAGCACTTTCAT+AGG + chr5_3:61718088-61718107 None:intergenic 40.0%
GACACGATTGAAGAATCTAG+AGG - chr5_3:61718162-61718181 Msa0823100:intron 40.0%
!! GCATTTTTGCTGTGACTCAA+AGG - chr5_3:61718353-61718372 Msa0823100:intron 40.0%
ACCTCTTGGAAGAATTGTTG+CGG - chr5_3:61718382-61718401 Msa0823100:intron 40.0%
!! CTCTTTGAAATTCTGAGGCA+TGG - chr5_3:61718406-61718425 Msa0823100:intron 40.0%
GAGGGTAATTGACACAAAGT+TGG + chr5_3:61718535-61718554 None:intergenic 40.0%
! CTGATTGATCACCCTTTTAG+AGG - chr5_3:61718732-61718751 Msa0823100:CDS 40.0%
TTTCCCCCTAAGTTCAAGAT+TGG - chr5_3:61719024-61719043 Msa0823100:CDS 40.0%
AGTCCAATCTTGAACTTAGG+GGG + chr5_3:61719030-61719049 None:intergenic 40.0%
CAGTCCAATCTTGAACTTAG+GGG + chr5_3:61719031-61719050 None:intergenic 40.0%
! ACACCGGTTAAATTCATCCT+TGG - chr5_3:61719091-61719110 Msa0823100:CDS 40.0%
AGTGAAGTGTGGAGTCTATA+TGG + chr5_3:61719222-61719241 None:intergenic 40.0%
AATGAAGTCGTAGTGAAGTG+TGG + chr5_3:61719233-61719252 None:intergenic 40.0%
CACTTCACTACGACTTCATT+AGG - chr5_3:61719233-61719252 Msa0823100:CDS 40.0%
TGTCTAGGGACTGAATTTAG+AGG - chr5_3:61719308-61719327 Msa0823100:CDS 40.0%
TCAAATAGTTGGGAGCTTGA+AGG - chr5_3:61719509-61719528 Msa0823100:intron 40.0%
CTCGATATACCTTTGCTCAT+GGG + chr5_3:61719542-61719561 None:intergenic 40.0%
!! GTTTTGGACTAAATAACCCG+CGG + chr5_3:61719753-61719772 None:intergenic 40.0%
GTATAGGCATGGTTACTGAC+TGG + chr5_3:61717073-61717092 None:intergenic 45.0%
!! GTAGGTGAAGAGTGTTGGTT+GGG + chr5_3:61717149-61717168 None:intergenic 45.0%
!! AGTAGGTGAAGAGTGTTGGT+TGG + chr5_3:61717150-61717169 None:intergenic 45.0%
! GAACAGTAGGTGAAGAGTGT+TGG + chr5_3:61717154-61717173 None:intergenic 45.0%
GAGAGAGTGATGACTACCTT+GGG + chr5_3:61717186-61717205 None:intergenic 45.0%
AGAGAGAGTGATGACTACCT+TGG + chr5_3:61717187-61717206 None:intergenic 45.0%
AAGAGGTTGGGAAAGGTAGA+TGG + chr5_3:61717211-61717230 None:intergenic 45.0%
ACATGTTGAGTCATGCAAGG+TGG - chr5_3:61717627-61717646 Msa0823100:intron 45.0%
TAAGGACATGACAGTAGCTG+AGG - chr5_3:61717879-61717898 Msa0823100:intron 45.0%
!! GAAGTTGCTTTGGAGACAAG+TGG - chr5_3:61717946-61717965 Msa0823100:intron 45.0%
!! TGGTGAGGGAATTGATGATG+AGG - chr5_3:61717966-61717985 Msa0823100:intron 45.0%
!! GGTGAGGGAATTGATGATGA+GGG - chr5_3:61717967-61717986 Msa0823100:intron 45.0%
TGTTCCTCCAAAGGGAATTG+AGG - chr5_3:61717999-61718018 Msa0823100:intron 45.0%
! GTCATTGGTGACATCTACAC+AGG - chr5_3:61718023-61718042 Msa0823100:intron 45.0%
TCCGCAACAATTCTTCCAAG+AGG + chr5_3:61718386-61718405 None:intergenic 45.0%
!! GCGGACTCTTTGAAATTCTG+AGG - chr5_3:61718401-61718420 Msa0823100:intron 45.0%
GGTAGAAGACCCAACAAGTT+TGG + chr5_3:61718955-61718974 None:intergenic 45.0%
TGGGTCTTCTACCCAAAGAT+TGG - chr5_3:61718962-61718981 Msa0823100:CDS 45.0%
AATCGATAGGCAACATGGCA+CGG - chr5_3:61719172-61719191 Msa0823100:CDS 45.0%
GCAGGCAGCATATTGTGAAT+AGG + chr5_3:61719389-61719408 None:intergenic 45.0%
AATAGTTGGGAGCTTGAAGG+AGG - chr5_3:61719512-61719531 Msa0823100:intron 45.0%
CCTCGATATACCTTTGCTCA+TGG + chr5_3:61719543-61719562 None:intergenic 45.0%
! CCATGAGCAAAGGTATATCG+AGG - chr5_3:61719540-61719559 Msa0823100:intron 45.0%
GTTGGGCAGAGTCAAAGGTT+TGG + chr5_3:61717132-61717151 None:intergenic 50.0%
! TGTTGGTTGGGCAGAGTCAA+AGG + chr5_3:61717137-61717156 None:intergenic 50.0%
TGTTGAGTCATGCAAGGTGG+TGG - chr5_3:61717630-61717649 Msa0823100:intron 50.0%
GGCCTCAGAAGCTGTAGAAT+TGG - chr5_3:61717729-61717748 Msa0823100:intron 50.0%
!! TGCTTTGGAGACAAGTGGTG+AGG - chr5_3:61717951-61717970 Msa0823100:intron 50.0%
!! GCTTTGGAGACAAGTGGTGA+GGG - chr5_3:61717952-61717971 Msa0823100:intron 50.0%
GACTCCTCAATTCCCTTTGG+AGG + chr5_3:61718006-61718025 None:intergenic 50.0%
CTCAAAGGCAACACACCTCT+TGG - chr5_3:61718368-61718387 Msa0823100:intron 50.0%
GGACATGACAGTAGCTGAGG+AGG - chr5_3:61717882-61717901 Msa0823100:intron 55.0%
GTTGGGAGCTTGAAGGAGGT+TGG - chr5_3:61719516-61719535 Msa0823100:intron 55.0%
CTGCAGTTCTCATGCTGGGA+GGG - chr5_3:61719565-61719584 Msa0823100:intron 55.0%
! GGAGGTTGGCCCATGAGCAA+AGG - chr5_3:61719530-61719549 Msa0823100:intron 60.0%
! AGGCGCTGCAGTTCTCATGC+TGG - chr5_3:61719560-61719579 Msa0823100:intron 60.0%
GGCGCTGCAGTTCTCATGCT+GGG - chr5_3:61719561-61719580 Msa0823100:intron 60.0%
GCTGCAGTTCTCATGCTGGG+AGG - chr5_3:61719564-61719583 Msa0823100:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 61716995 61719886 61716995 ID=Msa0823100;Name=Msa0823100
chr5_3 mRNA 61716995 61719886 61716995 ID=Msa0823100-mRNA-1;Parent=Msa0823100;Name=Msa0823100-mRNA-1;_AED=0.05;_eAED=0.05;_QI=88|1|1|1|0.25|0|5|349|319
chr5_3 exon 61716995 61717119 61716995 ID=Msa0823100-mRNA-1:exon:19085;Parent=Msa0823100-mRNA-1
chr5_3 exon 61717147 61717364 61717147 ID=Msa0823100-mRNA-1:exon:19084;Parent=Msa0823100-mRNA-1
chr5_3 exon 61718437 61718529 61718437 ID=Msa0823100-mRNA-1:exon:19083;Parent=Msa0823100-mRNA-1
chr5_3 exon 61718681 61719447 61718681 ID=Msa0823100-mRNA-1:exon:19082;Parent=Msa0823100-mRNA-1
chr5_3 exon 61719693 61719886 61719693 ID=Msa0823100-mRNA-1:exon:19081;Parent=Msa0823100-mRNA-1
chr5_3 five_prime_UTR 61719799 61719886 61719799 ID=Msa0823100-mRNA-1:five_prime_utr;Parent=Msa0823100-mRNA-1
chr5_3 CDS 61719693 61719798 61719693 ID=Msa0823100-mRNA-1:cds;Parent=Msa0823100-mRNA-1
chr5_3 CDS 61718681 61719447 61718681 ID=Msa0823100-mRNA-1:cds;Parent=Msa0823100-mRNA-1
chr5_3 CDS 61718443 61718529 61718443 ID=Msa0823100-mRNA-1:cds;Parent=Msa0823100-mRNA-1
chr5_3 three_prime_UTR 61718437 61718442 61718437 ID=Msa0823100-mRNA-1:three_prime_utr;Parent=Msa0823100-mRNA-1
chr5_3 three_prime_UTR 61717147 61717364 61717147 ID=Msa0823100-mRNA-1:three_prime_utr;Parent=Msa0823100-mRNA-1
chr5_3 three_prime_UTR 61716995 61717119 61716995 ID=Msa0823100-mRNA-1:three_prime_utr;Parent=Msa0823100-mRNA-1
Gene Sequence

>Msa0823100

ATGCCTATACCTTCAAGAAAAAAAACAAAACAAGCCAAGAACAAAACCAAACCTTTGACTCTGCCCAACCAACACTCTTCACCTACTGTTCAACTACAAACCCAAGCAGGTACATTTTTATCTGAAAATCATAGCAAAATGGAAGGAAATTTGAATGGAACATTGCATGAAACTCAAAAGGTTGAAGAAGATTCATTAAGTGAAGATAAGGAGGCAGCTGTTGAAGTATCTGCTGATGATGATTATGATGGAAAAGCCAAGTCTATCAGTGATACCAAAGATTTTGTTGATATTGATGAACATGTTGAGTCATGCAAGGTGGTGGAAGAGACAGAAGATGCTTCTGAAACAGTTTTTGAATCCAGTGATGCAATAGAAGTTAACCAAGTTGAGAATAAGATGGCCTCAGAAGCTGTAGAATTGGAATTGAATGATGAGAAAGGAGAAAATATTATATCCCTTGAAGATAATGATGAGGTGGAGGAAACTGAATTAGCTACTTCAAAGGATAATAATGAAGGATTTAAGGTAACTGAAGTGGTTGAAACAGTTAAGGACATGACAGTAGCTGAGGAGGAGAATGTTTTGCCTTCAACTGATGTGGTTTCATTTTCAAGGGAAGTTGCTTTGGAGACAAGTGGTGAGGGAATTGATGATGAGGGTGAAAAGGTTGTTCCTCCAAAGGGAATTGAGGAGTCATTGGTGACATCTACACAGGAGAAAGTTGAGGAACAGTTAGATGTTCAAGAATCATCATCCTATGAAAGTGCTAAGGAGTCACTTCAACAATCTTCAAATGTTCATGATTCTGAAAGCAATAGTGTCAATGAGCAAGACACGATTGAAGAATCTAGAGGCATTGAAAATCAAGGGAGCATTTTTGCTGTGACTCAAAGGCAACACACCTCTTGGAAGAATTGTTGCGGACTCTTTGAAATTCTGAGGCATGGTGATAGATAA
Protein sequence

>Msa0823100

MPIPSRKKTKQAKNKTKPLTLPNQHSSPTVQLQTQAGTFLSENHSKMEGNLNGTLHETQKVEEDSLSEDKEAAVEVSADDDYDGKAKSISDTKDFVDIDEHVESCKVVEETEDASETVFESSDAIEVNQVENKMASEAVELELNDEKGENIISLEDNDEVEETELATSKDNNEGFKVTEVVETVKDMTVAEEENVLPSTDVVSFSREVALETSGEGIDDEGEKVVPPKGIEESLVTSTQEKVEEQLDVQESSSYESAKESLQQSSNVHDSESNSVNEQDTIEESRGIENQGSIFAVTQRQHTSWKNCCGLFEILRHGDR*