AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0824740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0824740 MtrunA17_Chr5g0445581 94.595 74 4 0 4 77 3 76 4.84e-45 139
Msa0824740 MtrunA17_Chr2g0290831 42.188 64 37 0 3 66 2 65 7.58e-11 52.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0824740 AT4G05030.2 44.444 72 39 1 4 75 3 73 1.75e-11 54.7
Msa0824740 AT4G05030.1 45.070 71 38 1 5 75 37 106 5.80e-11 53.9

Find 18 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATAGAGTGGTGATTAA+AGG 0.272964 5_3:-64228524 Msa0824740:CDS
TATACATAGTTATTCATCAT+TGG 0.378479 5_3:+64228424 None:intergenic
AGGTGTTACAATGGTTACTT+TGG 0.427464 5_3:-64228558 Msa0824740:CDS
ACCATCAGCAATGGTACATA+TGG 0.447473 5_3:+64229547 None:intergenic
CAAGGGTGACTGAACGTTTG+AGG 0.485328 5_3:-64228484 Msa0824740:CDS
TACAATGGTTACTTTGGAAA+GGG 0.497733 5_3:-64228552 Msa0824740:CDS
TTACAATGGTTACTTTGGAA+AGG 0.504948 5_3:-64228553 Msa0824740:CDS
AAGGGTGACTGAACGTTTGA+GGG 0.506906 5_3:-64228483 Msa0824740:CDS
ACTAACAAGTCTAGCATGCC+TGG 0.525033 5_3:+64228451 None:intergenic
AAAATGCAAGAAGAAGATAT+TGG 0.540381 5_3:-64229570 Msa0824740:CDS
ATGGTTACTTTGGAAAGGGA+AGG 0.567959 5_3:-64228548 Msa0824740:CDS
GAGAAGATGTTGATGCAGCA+AGG 0.572875 5_3:-64228502 Msa0824740:CDS
AAGGGAAGGAAAAGATAGAG+TGG 0.595147 5_3:-64228534 Msa0824740:CDS
AGAAGATGTTGATGCAGCAA+GGG 0.596117 5_3:-64228501 Msa0824740:CDS
GAATGATATAGGTGTTACAA+TGG 0.641061 5_3:-64228567 Msa0824740:intron
GCCATATGTACCATTGCTGA+TGG 0.651621 5_3:-64229548 Msa0824740:intron
TCAACACATACCATCAGCAA+TGG 0.657350 5_3:+64229538 None:intergenic
GTTTGAGGGAGAAAGTGACC+AGG 0.682645 5_3:-64228469 Msa0824740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG - chr5_3:64228585-64228604 Msa0824740:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr5_3:64228584-64228603 Msa0824740:intron 0.0%
!!! TAAAATTTATTTTAATTAAA+AGG - chr5_3:64228989-64229008 Msa0824740:intron 0.0%
!! TATATATATATATATATATA+GGG + chr5_3:64228585-64228604 None:intergenic 0.0%
!! ATATATATATATATATATAT+AGG + chr5_3:64228584-64228603 None:intergenic 0.0%
!! TATATATATATATATATATA+TGG - chr5_3:64228585-64228604 Msa0824740:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr5_3:64228584-64228603 Msa0824740:intron 0.0%
!!! TAAAATTTATTTTAATTAAA+AGG - chr5_3:64228989-64229008 Msa0824740:intron 0.0%
!! TATATATATATATATATATA+GGG + chr5_3:64228585-64228604 None:intergenic 0.0%
!! ATATATATATATATATATAT+AGG + chr5_3:64228584-64228603 None:intergenic 0.0%
!! ATATATATATATATATATGG+GGG - chr5_3:64228630-64228649 Msa0824740:intron 10.0%
!! TATATATATATATATATAGG+GGG + chr5_3:64229142-64229161 None:intergenic 10.0%
!! ATATATAACAAATATTACAT+TGG - chr5_3:64229199-64229218 Msa0824740:intron 10.0%
!!! ATTTTTAACTTTTCAATATT+AGG - chr5_3:64229423-64229442 Msa0824740:intron 10.0%
!! ATATATATATATATATATGG+GGG - chr5_3:64228630-64228649 Msa0824740:intron 10.0%
!! TATATATATATATATATAGG+GGG + chr5_3:64229142-64229161 None:intergenic 10.0%
!! ATATATAACAAATATTACAT+TGG - chr5_3:64229199-64229218 Msa0824740:intron 10.0%
!!! ATTTTTAACTTTTCAATATT+AGG - chr5_3:64229423-64229442 Msa0824740:intron 10.0%
!! TTGTTTATAATAGAAAAACA+TGG - chr5_3:64228794-64228813 Msa0824740:intron 15.0%
!! TGTTTATAATAGAAAAACAT+GGG - chr5_3:64228795-64228814 Msa0824740:intron 15.0%
!!! AAATTTTAATAGAGACAAAA+GGG + chr5_3:64228977-64228996 None:intergenic 15.0%
!!! TAAATTTTAATAGAGACAAA+AGG + chr5_3:64228978-64228997 None:intergenic 15.0%
!! TTGTTTATAATAGAAAAACA+TGG - chr5_3:64228794-64228813 Msa0824740:intron 15.0%
!! TGTTTATAATAGAAAAACAT+GGG - chr5_3:64228795-64228814 Msa0824740:intron 15.0%
!! GAAAAAAGAAATATGTATTA+TGG + chr5_3:64229381-64229400 None:intergenic 15.0%
!! TTGTTTATAATAGAAAAACA+TGG - chr5_3:64228794-64228813 Msa0824740:intron 15.0%
!! TGTTTATAATAGAAAAACAT+GGG - chr5_3:64228795-64228814 Msa0824740:intron 15.0%
!!! AAATTTTAATAGAGACAAAA+GGG + chr5_3:64228977-64228996 None:intergenic 15.0%
!!! TAAATTTTAATAGAGACAAA+AGG + chr5_3:64228978-64228997 None:intergenic 15.0%
!! TTGTTTATAATAGAAAAACA+TGG - chr5_3:64228794-64228813 Msa0824740:intron 15.0%
!! TGTTTATAATAGAAAAACAT+GGG - chr5_3:64228795-64228814 Msa0824740:intron 15.0%
!! GAAAAAAGAAATATGTATTA+TGG + chr5_3:64229381-64229400 None:intergenic 15.0%
!! AAAGTTTAATAGAGACAAAA+AGG + chr5_3:64228731-64228750 None:intergenic 20.0%
!! AAAGTTTAATAGAGACAAAA+AGG + chr5_3:64228731-64228750 None:intergenic 20.0%
! AAAATGCAAGAAGAAGATAT+TGG - chr5_3:64228477-64228496 Msa0824740:CDS 25.0%
!!! TGTTTTAGTAAAGTACAAGT+AGG + chr5_3:64228708-64228727 None:intergenic 25.0%
! TGTTATGGTAAATAACTAGT+AGG - chr5_3:64228818-64228837 Msa0824740:intron 25.0%
!!! TGTTTTAGTAAAGTACAAGT+AGG + chr5_3:64228708-64228727 None:intergenic 25.0%
!!! TGTTTTGGTAAATAACTAGT+AGG - chr5_3:64229064-64229083 Msa0824740:intron 25.0%
! TAACAAATATTACATTGGTC+TGG - chr5_3:64229204-64229223 Msa0824740:intron 25.0%
! AAACCTTGTGATGAAAAATA+TGG - chr5_3:64229258-64229277 Msa0824740:intron 25.0%
!! TAACCATATTTTTCATCACA+AGG + chr5_3:64229264-64229283 None:intergenic 25.0%
! TGATGAAAAATATGGTTAAG+AGG - chr5_3:64229266-64229285 Msa0824740:intron 25.0%
!! AACTTTTCAATATTAGGAAC+TGG - chr5_3:64229429-64229448 Msa0824740:intron 25.0%
! AATATTAGGAACTGGTAATA+CGG - chr5_3:64229437-64229456 Msa0824740:intron 25.0%
! TAATCGTTTGTGAATGATAT+AGG - chr5_3:64229469-64229488 Msa0824740:intron 25.0%
! AAAATGCAAGAAGAAGATAT+TGG - chr5_3:64228477-64228496 Msa0824740:CDS 25.0%
!!! TGTTTTAGTAAAGTACAAGT+AGG + chr5_3:64228708-64228727 None:intergenic 25.0%
! TGTTATGGTAAATAACTAGT+AGG - chr5_3:64228818-64228837 Msa0824740:intron 25.0%
!!! TGTTTTAGTAAAGTACAAGT+AGG + chr5_3:64228708-64228727 None:intergenic 25.0%
!!! TGTTTTGGTAAATAACTAGT+AGG - chr5_3:64229064-64229083 Msa0824740:intron 25.0%
! TAACAAATATTACATTGGTC+TGG - chr5_3:64229204-64229223 Msa0824740:intron 25.0%
! AAACCTTGTGATGAAAAATA+TGG - chr5_3:64229258-64229277 Msa0824740:intron 25.0%
!! TAACCATATTTTTCATCACA+AGG + chr5_3:64229264-64229283 None:intergenic 25.0%
! TGATGAAAAATATGGTTAAG+AGG - chr5_3:64229266-64229285 Msa0824740:intron 25.0%
!! AACTTTTCAATATTAGGAAC+TGG - chr5_3:64229429-64229448 Msa0824740:intron 25.0%
! AATATTAGGAACTGGTAATA+CGG - chr5_3:64229437-64229456 Msa0824740:intron 25.0%
! TAATCGTTTGTGAATGATAT+AGG - chr5_3:64229469-64229488 Msa0824740:intron 25.0%
ATAGAAAAACATGGGTGTTA+TGG - chr5_3:64228803-64228822 Msa0824740:intron 30.0%
! ATAGAAAAACATGGGTGTTT+TGG - chr5_3:64229049-64229068 Msa0824740:intron 30.0%
! GAATGATATAGGTGTTACAA+TGG - chr5_3:64229480-64229499 Msa0824740:intron 30.0%
! TTACAATGGTTACTTTGGAA+AGG - chr5_3:64229494-64229513 Msa0824740:intron 30.0%
! TACAATGGTTACTTTGGAAA+GGG - chr5_3:64229495-64229514 Msa0824740:intron 30.0%
AAAGATAGAGTGGTGATTAA+AGG - chr5_3:64229523-64229542 Msa0824740:intron 30.0%
ATAGAAAAACATGGGTGTTA+TGG - chr5_3:64228803-64228822 Msa0824740:intron 30.0%
! ATAGAAAAACATGGGTGTTT+TGG - chr5_3:64229049-64229068 Msa0824740:intron 30.0%
! GAATGATATAGGTGTTACAA+TGG - chr5_3:64229480-64229499 Msa0824740:intron 30.0%
! TTACAATGGTTACTTTGGAA+AGG - chr5_3:64229494-64229513 Msa0824740:intron 30.0%
! TACAATGGTTACTTTGGAAA+GGG - chr5_3:64229495-64229514 Msa0824740:intron 30.0%
AAAGATAGAGTGGTGATTAA+AGG - chr5_3:64229523-64229542 Msa0824740:intron 30.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr5_3:64228853-64228872 None:intergenic 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG - chr5_3:64228853-64228872 Msa0824740:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr5_3:64228853-64228872 None:intergenic 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG - chr5_3:64228853-64228872 Msa0824740:intron 35.0%
GACTACACTAAAGATCAATC+AGG - chr5_3:64229292-64229311 Msa0824740:intron 35.0%
GAGGATGACTAATATTCATC+AGG + chr5_3:64229321-64229340 None:intergenic 35.0%
ATTAGTCATCCTCGAGAAAT+TGG - chr5_3:64229328-64229347 Msa0824740:intron 35.0%
! AGGTGTTACAATGGTTACTT+TGG - chr5_3:64229489-64229508 Msa0824740:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr5_3:64228853-64228872 None:intergenic 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG - chr5_3:64228853-64228872 Msa0824740:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr5_3:64228853-64228872 None:intergenic 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG - chr5_3:64228853-64228872 Msa0824740:intron 35.0%
GACTACACTAAAGATCAATC+AGG - chr5_3:64229292-64229311 Msa0824740:intron 35.0%
GAGGATGACTAATATTCATC+AGG + chr5_3:64229321-64229340 None:intergenic 35.0%
ATTAGTCATCCTCGAGAAAT+TGG - chr5_3:64229328-64229347 Msa0824740:intron 35.0%
! AGGTGTTACAATGGTTACTT+TGG - chr5_3:64229489-64229508 Msa0824740:intron 35.0%
! TCTTCTTGCATTTTGCACAG+TGG + chr5_3:64228471-64228490 None:intergenic 40.0%
ACCATCAGCAATGGTACATA+TGG + chr5_3:64228503-64228522 None:intergenic 40.0%
TCAACACATACCATCAGCAA+TGG + chr5_3:64228512-64228531 None:intergenic 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr5_3:64228857-64228876 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr5_3:64228856-64228875 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG - chr5_3:64228856-64228875 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG - chr5_3:64228857-64228876 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr5_3:64228857-64228876 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr5_3:64228856-64228875 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG - chr5_3:64228856-64228875 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG - chr5_3:64228857-64228876 Msa0824740:intron 40.0%
! ATGGTTACTTTGGAAAGGGA+AGG - chr5_3:64229499-64229518 Msa0824740:intron 40.0%
AAGGGAAGGAAAAGATAGAG+TGG - chr5_3:64229513-64229532 Msa0824740:intron 40.0%
AGAAGATGTTGATGCAGCAA+GGG - chr5_3:64229546-64229565 Msa0824740:intron 40.0%
! TCTTCTTGCATTTTGCACAG+TGG + chr5_3:64228471-64228490 None:intergenic 40.0%
ACCATCAGCAATGGTACATA+TGG + chr5_3:64228503-64228522 None:intergenic 40.0%
TCAACACATACCATCAGCAA+TGG + chr5_3:64228512-64228531 None:intergenic 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr5_3:64228857-64228876 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr5_3:64228856-64228875 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG - chr5_3:64228856-64228875 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG - chr5_3:64228857-64228876 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr5_3:64228857-64228876 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr5_3:64228856-64228875 None:intergenic 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG - chr5_3:64228856-64228875 Msa0824740:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG - chr5_3:64228857-64228876 Msa0824740:intron 40.0%
! ATGGTTACTTTGGAAAGGGA+AGG - chr5_3:64229499-64229518 Msa0824740:intron 40.0%
AAGGGAAGGAAAAGATAGAG+TGG - chr5_3:64229513-64229532 Msa0824740:intron 40.0%
AGAAGATGTTGATGCAGCAA+GGG - chr5_3:64229546-64229565 Msa0824740:intron 40.0%
! TTGCATTTTGCACAGTGGAG+GGG + chr5_3:64228466-64228485 None:intergenic 45.0%
! CTTGCATTTTGCACAGTGGA+GGG + chr5_3:64228467-64228486 None:intergenic 45.0%
! TCTTGCATTTTGCACAGTGG+AGG + chr5_3:64228468-64228487 None:intergenic 45.0%
GCCATATGTACCATTGCTGA+TGG - chr5_3:64228499-64228518 Msa0824740:CDS 45.0%
GCACCCACCTAAAAACACTA+AGG - chr5_3:64228663-64228682 Msa0824740:intron 45.0%
GCACCCACCTAAAAACACTA+AGG + chr5_3:64228663-64228682 None:intergenic 45.0%
GCACCCACCTAAAAACACTA+AGG - chr5_3:64228663-64228682 Msa0824740:intron 45.0%
GGACTCGAATCGCTTAAACA+AGG - chr5_3:64229225-64229244 Msa0824740:intron 45.0%
TGAGCATCTCCAATTTCTCG+AGG + chr5_3:64229340-64229359 None:intergenic 45.0%
GAGAAGATGTTGATGCAGCA+AGG - chr5_3:64229545-64229564 Msa0824740:intron 45.0%
AAGGGTGACTGAACGTTTGA+GGG - chr5_3:64229564-64229583 Msa0824740:CDS 45.0%
ACTAACAAGTCTAGCATGCC+TGG + chr5_3:64229599-64229618 None:intergenic 45.0%
! TTGCATTTTGCACAGTGGAG+GGG + chr5_3:64228466-64228485 None:intergenic 45.0%
! CTTGCATTTTGCACAGTGGA+GGG + chr5_3:64228467-64228486 None:intergenic 45.0%
! TCTTGCATTTTGCACAGTGG+AGG + chr5_3:64228468-64228487 None:intergenic 45.0%
GCCATATGTACCATTGCTGA+TGG - chr5_3:64228499-64228518 Msa0824740:CDS 45.0%
GCACCCACCTAAAAACACTA+AGG - chr5_3:64228663-64228682 Msa0824740:intron 45.0%
GCACCCACCTAAAAACACTA+AGG + chr5_3:64228663-64228682 None:intergenic 45.0%
GCACCCACCTAAAAACACTA+AGG - chr5_3:64228663-64228682 Msa0824740:intron 45.0%
GGACTCGAATCGCTTAAACA+AGG - chr5_3:64229225-64229244 Msa0824740:intron 45.0%
TGAGCATCTCCAATTTCTCG+AGG + chr5_3:64229340-64229359 None:intergenic 45.0%
GAGAAGATGTTGATGCAGCA+AGG - chr5_3:64229545-64229564 Msa0824740:intron 45.0%
AAGGGTGACTGAACGTTTGA+GGG - chr5_3:64229564-64229583 Msa0824740:CDS 45.0%
ACTAACAAGTCTAGCATGCC+TGG + chr5_3:64229599-64229618 None:intergenic 45.0%
!! TATATATATATATATATATG+GGG - chr5_3:64228629-64228648 Msa0824740:intron 5.0%
!!! TAAACTTTATTTTAATTAAA+AGG - chr5_3:64228743-64228762 Msa0824740:intron 5.0%
!! ATATATATATATATATATAG+GGG + chr5_3:64229143-64229162 None:intergenic 5.0%
!! TATATATATATATATATATG+GGG - chr5_3:64228629-64228648 Msa0824740:intron 5.0%
!!! TAAACTTTATTTTAATTAAA+AGG - chr5_3:64228743-64228762 Msa0824740:intron 5.0%
!! ATATATATATATATATATAG+GGG + chr5_3:64229143-64229162 None:intergenic 5.0%
AGGTGGGTGCAAGTTAACAG+TGG - chr5_3:64228873-64228892 Msa0824740:intron 50.0%
GGTGGGTGCAAGTTAACAGT+GGG - chr5_3:64228874-64228893 Msa0824740:intron 50.0%
GTGGGTGCAAGTTAACAGTG+GGG - chr5_3:64228875-64228894 Msa0824740:intron 50.0%
TGGGTGCAAGTTAACAGTGG+GGG - chr5_3:64228876-64228895 Msa0824740:intron 50.0%
GCGCCCACCTAAAAACACTA+AGG + chr5_3:64229109-64229128 None:intergenic 50.0%
CAAGGGTGACTGAACGTTTG+AGG - chr5_3:64229563-64229582 Msa0824740:CDS 50.0%
GTTTGAGGGAGAAAGTGACC+AGG - chr5_3:64229578-64229597 Msa0824740:CDS 50.0%
AGGTGGGTGCAAGTTAACAG+TGG - chr5_3:64228873-64228892 Msa0824740:intron 50.0%
GGTGGGTGCAAGTTAACAGT+GGG - chr5_3:64228874-64228893 Msa0824740:intron 50.0%
GTGGGTGCAAGTTAACAGTG+GGG - chr5_3:64228875-64228894 Msa0824740:intron 50.0%
TGGGTGCAAGTTAACAGTGG+GGG - chr5_3:64228876-64228895 Msa0824740:intron 50.0%
GCGCCCACCTAAAAACACTA+AGG + chr5_3:64229109-64229128 None:intergenic 50.0%
CAAGGGTGACTGAACGTTTG+AGG - chr5_3:64229563-64229582 Msa0824740:CDS 50.0%
GTTTGAGGGAGAAAGTGACC+AGG - chr5_3:64229578-64229597 Msa0824740:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 64228433 64229636 64228433 ID=Msa0824740;Name=Msa0824740
chr5_3 mRNA 64228433 64229636 64228433 ID=Msa0824740-mRNA-1;Parent=Msa0824740;Name=Msa0824740-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|77
chr5_3 exon 64229549 64229636 64229549 ID=Msa0824740-mRNA-1:exon:20056;Parent=Msa0824740-mRNA-1
chr5_3 exon 64228433 64228578 64228433 ID=Msa0824740-mRNA-1:exon:20055;Parent=Msa0824740-mRNA-1
chr5_3 CDS 64229549 64229636 64229549 ID=Msa0824740-mRNA-1:cds;Parent=Msa0824740-mRNA-1
chr5_3 CDS 64228433 64228578 64228433 ID=Msa0824740-mRNA-1:cds;Parent=Msa0824740-mRNA-1
Gene Sequence

>Msa0824740

ATGGCGCAGCAAAAGATCGTGATTGAGTTGCCCCTCCACTGTGCAAAATGCAAGAAGAAGATATTGGCCATATGTACCATTGCTGATGGTGTTACAATGGTTACTTTGGAAAGGGAAGGAAAAGATAGAGTGGTGATTAAAGGAGAAGATGTTGATGCAGCAAGGGTGACTGAACGTTTGAGGGAGAAAGTGACCAGGCATGCTAGACTTGTTAGTGTGACCAATGATGAATAA

Protein sequence

>Msa0824740

MAQQKIVIELPLHCAKCKKKILAICTIADGVTMVTLEREGKDRVVIKGEDVDAARVTERLREKVTRHARLVSVTNDE*