AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0828690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0828690 MtrunA17_Chr3g0080441 67.857 56 13 1 5 55 2 57 2.72e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAATCAATTAATTATTT+TGG 0.141252 5_4:-1594337 None:intergenic
TTCAAAGAGGTGATATAGTA+TGG 0.249719 5_4:+1593420 Msa0828690:CDS
AAGGTGCATTGTGGGGTGTT+GGG 0.335380 5_4:-1593533 None:intergenic
AACTTCAGATTCGACCAAAA+AGG 0.375842 5_4:-1593552 None:intergenic
TTGGAAGATGTGAGCAAAGC+TGG 0.426119 5_4:-1593476 None:intergenic
AGGTGCATTGTGGGGTGTTG+GGG 0.451372 5_4:-1593532 None:intergenic
AAAGGTGCATTGTGGGGTGT+TGG 0.451525 5_4:-1593534 None:intergenic
CTCACATCTTCCAACGATCT+CGG 0.456832 5_4:+1593485 Msa0828690:CDS
CCTCTTGCTCGTCTTGAGCT+AGG 0.469882 5_4:+1593832 Msa0828690:CDS
TAATTCACTTCCCTCAGAAC+AGG 0.474692 5_4:+1593450 Msa0828690:CDS
GAGTGAGATGCCGAGATCGT+TGG 0.518357 5_4:-1593495 None:intergenic
AAGCTGGAGACCTGTTCTGA+GGG 0.557278 5_4:-1593460 None:intergenic
CAATTATAGCAGAACAAGCG+TGG 0.564339 5_4:+1594200 Msa0828690:intron
TCTTTGAACATTCCTCGTCA+TGG 0.566483 5_4:-1593405 None:intergenic
CCTAGCTCAAGACGAGCAAG+AGG 0.574921 5_4:-1593832 None:intergenic
CAAGCGTGGGAACCGAAAGA+TGG 0.582509 5_4:+1594214 Msa0828690:CDS
AAAGCTGGAGACCTGTTCTG+AGG 0.589143 5_4:-1593461 None:intergenic
GATTCAACCTCAGTCGTAGG+AGG 0.605163 5_4:-1594284 None:intergenic
GAGCATTCCTCCTACGACTG+AGG 0.605605 5_4:+1594277 Msa0828690:CDS
TCAAAGAGGTGATATAGTAT+GGG 0.609657 5_4:+1593421 Msa0828690:CDS
TCAGATTCAACCTCAGTCGT+AGG 0.616826 5_4:-1594287 None:intergenic
AATTATAGCAGAACAAGCGT+GGG 0.679794 5_4:+1594201 Msa0828690:intron
ATGACGAGGAATGTTCAAAG+AGG 0.692384 5_4:+1593407 Msa0828690:CDS
AAACAGACATAACCATGACG+AGG 0.720821 5_4:+1593393 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTCTTCTTTTTTGAAA+CGG - chr5_4:1593880-1593899 None:intergenic 20.0%
!!! GATTTTCTTCTTTTTTGAAA+CGG - chr5_4:1593880-1593899 None:intergenic 20.0%
!!! TTATTTGCTGTCTTTTTTAG+TGG - chr5_4:1593938-1593957 None:intergenic 25.0%
!! TTGCATTTTATTATCATTGC+TGG - chr5_4:1594165-1594184 None:intergenic 25.0%
!!! TTTTTTCAATGAATTCAGAC+TGG - chr5_4:1594256-1594275 None:intergenic 25.0%
!!! TTATTTGCTGTCTTTTTTAG+TGG - chr5_4:1593938-1593957 None:intergenic 25.0%
!! TTGCATTTTATTATCATTGC+TGG - chr5_4:1594165-1594184 None:intergenic 25.0%
!!! TTTTTTCAATGAATTCAGAC+TGG - chr5_4:1594256-1594275 None:intergenic 25.0%
TTCAAAGAGGTGATATAGTA+TGG + chr5_4:1593420-1593439 Msa0828690:CDS 30.0%
TCAAAGAGGTGATATAGTAT+GGG + chr5_4:1593421-1593440 Msa0828690:CDS 30.0%
TGAAAGGGTGATTTATTGAA+GGG - chr5_4:1593581-1593600 None:intergenic 30.0%
TTGAAAGGGTGATTTATTGA+AGG - chr5_4:1593582-1593601 None:intergenic 30.0%
ATAACTATAAACGAGCAGAA+TGG - chr5_4:1593631-1593650 None:intergenic 30.0%
AAAAGACAACTAAGATAGCA+AGG + chr5_4:1594024-1594043 Msa0828690:intron 30.0%
!!! TATTTTGATATGGTGGTGAA+TGG - chr5_4:1594079-1594098 None:intergenic 30.0%
!!! TGTTTGGTATTTTGATATGG+TGG - chr5_4:1594086-1594105 None:intergenic 30.0%
!!! CTCTGTTTGGTATTTTGATA+TGG - chr5_4:1594089-1594108 None:intergenic 30.0%
!!! TTTTGGCTTGTTATTTCTGT+AGG - chr5_4:1594323-1594342 None:intergenic 30.0%
TTCAAAGAGGTGATATAGTA+TGG + chr5_4:1593420-1593439 Msa0828690:CDS 30.0%
TCAAAGAGGTGATATAGTAT+GGG + chr5_4:1593421-1593440 Msa0828690:CDS 30.0%
TGAAAGGGTGATTTATTGAA+GGG - chr5_4:1593581-1593600 None:intergenic 30.0%
TTGAAAGGGTGATTTATTGA+AGG - chr5_4:1593582-1593601 None:intergenic 30.0%
ATAACTATAAACGAGCAGAA+TGG - chr5_4:1593631-1593650 None:intergenic 30.0%
AAAAGACAACTAAGATAGCA+AGG + chr5_4:1594024-1594043 Msa0828690:intron 30.0%
!!! TATTTTGATATGGTGGTGAA+TGG - chr5_4:1594079-1594098 None:intergenic 30.0%
!!! TGTTTGGTATTTTGATATGG+TGG - chr5_4:1594086-1594105 None:intergenic 30.0%
!!! CTCTGTTTGGTATTTTGATA+TGG - chr5_4:1594089-1594108 None:intergenic 30.0%
!!! TTTTGGCTTGTTATTTCTGT+AGG - chr5_4:1594323-1594342 None:intergenic 30.0%
AACTTCAGATTCGACCAAAA+AGG - chr5_4:1593555-1593574 None:intergenic 35.0%
GAAAGGGTGATTTATTGAAG+GGG - chr5_4:1593580-1593599 None:intergenic 35.0%
!!! TGAATGGTGTCGTTTTGAAA+GGG - chr5_4:1593596-1593615 None:intergenic 35.0%
!!! TTGAATGGTGTCGTTTTGAA+AGG - chr5_4:1593597-1593616 None:intergenic 35.0%
ATGGAATGCTTCACATTGAA+TGG - chr5_4:1593612-1593631 None:intergenic 35.0%
! TTTACAGTGTCGCTGTATAA+TGG + chr5_4:1593673-1593692 Msa0828690:intron 35.0%
! TTACAGTGTCGCTGTATAAT+GGG + chr5_4:1593674-1593693 Msa0828690:intron 35.0%
! TATAATGGGACATAACGAAG+AGG + chr5_4:1593688-1593707 Msa0828690:intron 35.0%
! TTGTTGTAGCAGTGTGTTTT+TGG + chr5_4:1593776-1593795 Msa0828690:intron 35.0%
!!! TAGCAGTGTGTTTTTGGTTT+TGG + chr5_4:1593782-1593801 Msa0828690:intron 35.0%
! CTAGTTATGTTGGTTAGATG+TGG - chr5_4:1593986-1594005 None:intergenic 35.0%
CACATCTAACCAACATAACT+AGG + chr5_4:1593984-1594003 Msa0828690:intron 35.0%
!!! CTAGTTTTGCCTAGTTATGT+TGG - chr5_4:1593996-1594015 None:intergenic 35.0%
CAACATAACTAGGCAAAACT+AGG + chr5_4:1593994-1594013 Msa0828690:intron 35.0%
! TGGAGAATGTTGTGTCATTA+TGG - chr5_4:1594059-1594078 None:intergenic 35.0%
!! TTTGTTTAGCTCTCTCTGTT+TGG - chr5_4:1594102-1594121 None:intergenic 35.0%
AATTATAGCAGAACAAGCGT+GGG + chr5_4:1594201-1594220 Msa0828690:intron 35.0%
!! TCTGTTTTTCAGCCATCTTT+CGG - chr5_4:1594229-1594248 None:intergenic 35.0%
AACTTCAGATTCGACCAAAA+AGG - chr5_4:1593555-1593574 None:intergenic 35.0%
GAAAGGGTGATTTATTGAAG+GGG - chr5_4:1593580-1593599 None:intergenic 35.0%
!!! TGAATGGTGTCGTTTTGAAA+GGG - chr5_4:1593596-1593615 None:intergenic 35.0%
!!! TTGAATGGTGTCGTTTTGAA+AGG - chr5_4:1593597-1593616 None:intergenic 35.0%
ATGGAATGCTTCACATTGAA+TGG - chr5_4:1593612-1593631 None:intergenic 35.0%
! TTTACAGTGTCGCTGTATAA+TGG + chr5_4:1593673-1593692 Msa0828690:intron 35.0%
! TTACAGTGTCGCTGTATAAT+GGG + chr5_4:1593674-1593693 Msa0828690:intron 35.0%
! TATAATGGGACATAACGAAG+AGG + chr5_4:1593688-1593707 Msa0828690:intron 35.0%
! TTGTTGTAGCAGTGTGTTTT+TGG + chr5_4:1593776-1593795 Msa0828690:intron 35.0%
!!! TAGCAGTGTGTTTTTGGTTT+TGG + chr5_4:1593782-1593801 Msa0828690:intron 35.0%
! CTAGTTATGTTGGTTAGATG+TGG - chr5_4:1593986-1594005 None:intergenic 35.0%
CACATCTAACCAACATAACT+AGG + chr5_4:1593984-1594003 Msa0828690:intron 35.0%
!!! CTAGTTTTGCCTAGTTATGT+TGG - chr5_4:1593996-1594015 None:intergenic 35.0%
CAACATAACTAGGCAAAACT+AGG + chr5_4:1593994-1594013 Msa0828690:intron 35.0%
! TGGAGAATGTTGTGTCATTA+TGG - chr5_4:1594059-1594078 None:intergenic 35.0%
!! TTTGTTTAGCTCTCTCTGTT+TGG - chr5_4:1594102-1594121 None:intergenic 35.0%
AATTATAGCAGAACAAGCGT+GGG + chr5_4:1594201-1594220 Msa0828690:intron 35.0%
!! TCTGTTTTTCAGCCATCTTT+CGG - chr5_4:1594229-1594248 None:intergenic 35.0%
ATGACGAGGAATGTTCAAAG+AGG + chr5_4:1593407-1593426 Msa0828690:CDS 40.0%
TAATTCACTTCCCTCAGAAC+AGG + chr5_4:1593450-1593469 Msa0828690:CDS 40.0%
!!! GAAGAGGAGAGGGTTTTAAA+TGG + chr5_4:1593704-1593723 Msa0828690:intron 40.0%
!!! GAGAGGGTTTTAAATGGTTC+AGG + chr5_4:1593710-1593729 Msa0828690:intron 40.0%
!!! CAGTGTGTTTTTGGTTTTGG+TGG + chr5_4:1593785-1593804 Msa0828690:intron 40.0%
CACGCTTGTTCTGCTATAAT+TGG - chr5_4:1594202-1594221 None:intergenic 40.0%
CAATTATAGCAGAACAAGCG+TGG + chr5_4:1594200-1594219 Msa0828690:intron 40.0%
ATGACGAGGAATGTTCAAAG+AGG + chr5_4:1593407-1593426 Msa0828690:CDS 40.0%
TAATTCACTTCCCTCAGAAC+AGG + chr5_4:1593450-1593469 Msa0828690:CDS 40.0%
!!! GAAGAGGAGAGGGTTTTAAA+TGG + chr5_4:1593704-1593723 Msa0828690:intron 40.0%
!!! GAGAGGGTTTTAAATGGTTC+AGG + chr5_4:1593710-1593729 Msa0828690:intron 40.0%
!!! CAGTGTGTTTTTGGTTTTGG+TGG + chr5_4:1593785-1593804 Msa0828690:intron 40.0%
CACGCTTGTTCTGCTATAAT+TGG - chr5_4:1594202-1594221 None:intergenic 40.0%
CAATTATAGCAGAACAAGCG+TGG + chr5_4:1594200-1594219 Msa0828690:intron 40.0%
TTGGAAGATGTGAGCAAAGC+TGG - chr5_4:1593479-1593498 None:intergenic 45.0%
CTCACATCTTCCAACGATCT+CGG + chr5_4:1593485-1593504 Msa0828690:CDS 45.0%
! GACCAAAAAGGTGCATTGTG+GGG - chr5_4:1593543-1593562 None:intergenic 45.0%
! CGACCAAAAAGGTGCATTGT+GGG - chr5_4:1593544-1593563 None:intergenic 45.0%
! TCGACCAAAAAGGTGCATTG+TGG - chr5_4:1593545-1593564 None:intergenic 45.0%
! TTCAGGTTACCAGTTTGACC+CGG + chr5_4:1593727-1593746 Msa0828690:intron 45.0%
! TTTGACCCGGTTGGAGTTTT+GGG + chr5_4:1593740-1593759 Msa0828690:intron 45.0%
TGATACCCAAAACTCCAACC+GGG - chr5_4:1593748-1593767 None:intergenic 45.0%
CTGATACCCAAAACTCCAAC+CGG - chr5_4:1593749-1593768 None:intergenic 45.0%
!!! GTGTTTTTGGTTTTGGTGGC+AGG + chr5_4:1593789-1593808 Msa0828690:intron 45.0%
GTGGCTGTATGACAGTTAGT+AGG - chr5_4:1594135-1594154 None:intergenic 45.0%
TCAGATTCAACCTCAGTCGT+AGG - chr5_4:1594290-1594309 None:intergenic 45.0%
TTGGAAGATGTGAGCAAAGC+TGG - chr5_4:1593479-1593498 None:intergenic 45.0%
CTCACATCTTCCAACGATCT+CGG + chr5_4:1593485-1593504 Msa0828690:CDS 45.0%
! GACCAAAAAGGTGCATTGTG+GGG - chr5_4:1593543-1593562 None:intergenic 45.0%
! CGACCAAAAAGGTGCATTGT+GGG - chr5_4:1593544-1593563 None:intergenic 45.0%
! TCGACCAAAAAGGTGCATTG+TGG - chr5_4:1593545-1593564 None:intergenic 45.0%
! TTCAGGTTACCAGTTTGACC+CGG + chr5_4:1593727-1593746 Msa0828690:intron 45.0%
! TTTGACCCGGTTGGAGTTTT+GGG + chr5_4:1593740-1593759 Msa0828690:intron 45.0%
TGATACCCAAAACTCCAACC+GGG - chr5_4:1593748-1593767 None:intergenic 45.0%
CTGATACCCAAAACTCCAAC+CGG - chr5_4:1593749-1593768 None:intergenic 45.0%
!!! GTGTTTTTGGTTTTGGTGGC+AGG + chr5_4:1593789-1593808 Msa0828690:intron 45.0%
GTGGCTGTATGACAGTTAGT+AGG - chr5_4:1594135-1594154 None:intergenic 45.0%
TCAGATTCAACCTCAGTCGT+AGG - chr5_4:1594290-1594309 None:intergenic 45.0%
AAGCTGGAGACCTGTTCTGA+GGG - chr5_4:1593463-1593482 None:intergenic 50.0%
AAAGCTGGAGACCTGTTCTG+AGG - chr5_4:1593464-1593483 None:intergenic 50.0%
! AAGGTGCATTGTGGGGTGTT+GGG - chr5_4:1593536-1593555 None:intergenic 50.0%
! AAAGGTGCATTGTGGGGTGT+TGG - chr5_4:1593537-1593556 None:intergenic 50.0%
! CACCCCACAATGCACCTTTT+TGG + chr5_4:1593538-1593557 Msa0828690:CDS 50.0%
TGGGACATAACGAAGAGGAG+AGG + chr5_4:1593693-1593712 Msa0828690:intron 50.0%
GGGACATAACGAAGAGGAGA+GGG + chr5_4:1593694-1593713 Msa0828690:intron 50.0%
AAACTCCAACCGGGTCAAAC+TGG - chr5_4:1593739-1593758 None:intergenic 50.0%
! GTTTGACCCGGTTGGAGTTT+TGG + chr5_4:1593739-1593758 Msa0828690:intron 50.0%
! TATCATTGCTGGCTGCACTG+TGG - chr5_4:1594154-1594173 None:intergenic 50.0%
GATTCAACCTCAGTCGTAGG+AGG - chr5_4:1594287-1594306 None:intergenic 50.0%
AAGCTGGAGACCTGTTCTGA+GGG - chr5_4:1593463-1593482 None:intergenic 50.0%
AAAGCTGGAGACCTGTTCTG+AGG - chr5_4:1593464-1593483 None:intergenic 50.0%
! AAGGTGCATTGTGGGGTGTT+GGG - chr5_4:1593536-1593555 None:intergenic 50.0%
! AAAGGTGCATTGTGGGGTGT+TGG - chr5_4:1593537-1593556 None:intergenic 50.0%
! CACCCCACAATGCACCTTTT+TGG + chr5_4:1593538-1593557 Msa0828690:CDS 50.0%
TGGGACATAACGAAGAGGAG+AGG + chr5_4:1593693-1593712 Msa0828690:intron 50.0%
GGGACATAACGAAGAGGAGA+GGG + chr5_4:1593694-1593713 Msa0828690:intron 50.0%
AAACTCCAACCGGGTCAAAC+TGG - chr5_4:1593739-1593758 None:intergenic 50.0%
! GTTTGACCCGGTTGGAGTTT+TGG + chr5_4:1593739-1593758 Msa0828690:intron 50.0%
! TATCATTGCTGGCTGCACTG+TGG - chr5_4:1594154-1594173 None:intergenic 50.0%
GATTCAACCTCAGTCGTAGG+AGG - chr5_4:1594287-1594306 None:intergenic 50.0%
GAGTGAGATGCCGAGATCGT+TGG - chr5_4:1593498-1593517 None:intergenic 55.0%
!! AGGTGCATTGTGGGGTGTTG+GGG - chr5_4:1593535-1593554 None:intergenic 55.0%
GGTTACCAGTTTGACCCGGT+TGG + chr5_4:1593731-1593750 Msa0828690:intron 55.0%
CCTAGCTCAAGACGAGCAAG+AGG - chr5_4:1593835-1593854 None:intergenic 55.0%
CCTCTTGCTCGTCTTGAGCT+AGG + chr5_4:1593832-1593851 Msa0828690:CDS 55.0%
CAAGCGTGGGAACCGAAAGA+TGG + chr5_4:1594214-1594233 Msa0828690:CDS 55.0%
GAGCATTCCTCCTACGACTG+AGG + chr5_4:1594277-1594296 Msa0828690:CDS 55.0%
GAGTGAGATGCCGAGATCGT+TGG - chr5_4:1593498-1593517 None:intergenic 55.0%
!! AGGTGCATTGTGGGGTGTTG+GGG - chr5_4:1593535-1593554 None:intergenic 55.0%
GGTTACCAGTTTGACCCGGT+TGG + chr5_4:1593731-1593750 Msa0828690:intron 55.0%
CCTAGCTCAAGACGAGCAAG+AGG - chr5_4:1593835-1593854 None:intergenic 55.0%
CCTCTTGCTCGTCTTGAGCT+AGG + chr5_4:1593832-1593851 Msa0828690:CDS 55.0%
CAAGCGTGGGAACCGAAAGA+TGG + chr5_4:1594214-1594233 Msa0828690:CDS 55.0%
GAGCATTCCTCCTACGACTG+AGG + chr5_4:1594277-1594296 Msa0828690:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 1593407 1594349 1593407 ID=Msa0828690;Name=Msa0828690
chr5_4 mRNA 1593407 1594349 1593407 ID=Msa0828690-mRNA-1;Parent=Msa0828690;Name=Msa0828690-mRNA-1;_AED=0.75;_eAED=0.75;_QI=0|0|0|0.33|1|1|3|0|116
chr5_4 exon 1593407 1593571 1593407 ID=Msa0828690-mRNA-1:exon:409;Parent=Msa0828690-mRNA-1
chr5_4 exon 1593811 1593858 1593811 ID=Msa0828690-mRNA-1:exon:410;Parent=Msa0828690-mRNA-1
chr5_4 exon 1594212 1594349 1594212 ID=Msa0828690-mRNA-1:exon:411;Parent=Msa0828690-mRNA-1
chr5_4 CDS 1593407 1593571 1593407 ID=Msa0828690-mRNA-1:cds;Parent=Msa0828690-mRNA-1
chr5_4 CDS 1593811 1593858 1593811 ID=Msa0828690-mRNA-1:cds;Parent=Msa0828690-mRNA-1
chr5_4 CDS 1594212 1594349 1594212 ID=Msa0828690-mRNA-1:cds;Parent=Msa0828690-mRNA-1
Gene Sequence

>Msa0828690

ATGACGAGGAATGTTCAAAGAGGTGATATAGTATGGGTAAGAATAATTCACTTCCCTCAGAACAGGTCTCCAGCTTTGCTCACATCTTCCAACGATCTCGGCATCTCACTCTCTTTCTTCAACAACCCCAACACCCCACAATGCACCTTTTTGGTCGAATCTGAAGCTGCAGTTCTGTTTATTGCGCCTCTTGCTCGTCTTGAGCTAGGTAGTAACAAGCGTGGGAACCGAAAGATGGCTGAAAAACAGAAAGTCCAGTCTGAATTCATTGAAAAAATGAGCATTCCTCCTACGACTGAGGTTGAATCTGATCTGAAAAGACCTACAGAAATAACAAGCCAAAATAATTAA

Protein sequence

>Msa0828690

MTRNVQRGDIVWVRIIHFPQNRSPALLTSSNDLGISLSFFNNPNTPQCTFLVESEAAVLFIAPLARLELGSNKRGNRKMAEKQKVQSEFIEKMSIPPTTEVESDLKRPTEITSQNN*