AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0397900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0397900 MtrunA17_Chr3g0080441 67.857 56 13 1 5 55 2 57 2.72e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAATCAATTAATTATTT+TGG 0.141252 3_2:+18067127 None:intergenic
TTCAAAGAGGTGATATAGTA+TGG 0.249719 3_2:-18068044 Msa0397900:CDS
AAGGTGCATTGTGGGGTGTT+GGG 0.335380 3_2:+18067931 None:intergenic
AACTTCAGATTCGACCAAAA+AGG 0.375842 3_2:+18067912 None:intergenic
TTGGAAGATGTGAGCAAAGC+TGG 0.426119 3_2:+18067988 None:intergenic
AGGTGCATTGTGGGGTGTTG+GGG 0.451372 3_2:+18067932 None:intergenic
AAAGGTGCATTGTGGGGTGT+TGG 0.451525 3_2:+18067930 None:intergenic
CTCACATCTTCCAACGATCT+CGG 0.456832 3_2:-18067979 Msa0397900:CDS
CCTCTTGCTCGTCTTGAGCT+AGG 0.469882 3_2:-18067632 Msa0397900:CDS
TAATTCACTTCCCTCAGAAC+AGG 0.474692 3_2:-18068014 Msa0397900:CDS
GAGTGAGATGCCGAGATCGT+TGG 0.518357 3_2:+18067969 None:intergenic
AAGCTGGAGACCTGTTCTGA+GGG 0.557278 3_2:+18068004 None:intergenic
CAATTATAGCAGAACAAGCG+TGG 0.564339 3_2:-18067264 Msa0397900:intron
TCTTTGAACATTCCTCGTCA+TGG 0.566483 3_2:+18068059 None:intergenic
CCTAGCTCAAGACGAGCAAG+AGG 0.574921 3_2:+18067632 None:intergenic
CAAGCGTGGGAACCGAAAGA+TGG 0.582509 3_2:-18067250 Msa0397900:CDS
AAAGCTGGAGACCTGTTCTG+AGG 0.589143 3_2:+18068003 None:intergenic
GATTCAACCTCAGTCGTAGG+AGG 0.605163 3_2:+18067180 None:intergenic
GAGCATTCCTCCTACGACTG+AGG 0.605605 3_2:-18067187 Msa0397900:CDS
TCAAAGAGGTGATATAGTAT+GGG 0.609657 3_2:-18068043 Msa0397900:CDS
TCAGATTCAACCTCAGTCGT+AGG 0.616826 3_2:+18067177 None:intergenic
AATTATAGCAGAACAAGCGT+GGG 0.679794 3_2:-18067263 Msa0397900:intron
ATGACGAGGAATGTTCAAAG+AGG 0.692384 3_2:-18068057 Msa0397900:CDS
AAACAGACATAACCATGACG+AGG 0.720821 3_2:-18068071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTCTTCTTTTTTGAAA+CGG + chr3_2:18067610-18067629 None:intergenic 20.0%
!!! GATTTTCTTCTTTTTTGAAA+CGG + chr3_2:18067610-18067629 None:intergenic 20.0%
!!! TTATTTGCTGTCTTTTTTAG+TGG + chr3_2:18067668-18067687 None:intergenic 25.0%
!! TTGCATTTTATTATCATTGC+TGG + chr3_2:18067895-18067914 None:intergenic 25.0%
!!! TTTTTTCAATGAATTCAGAC+TGG + chr3_2:18067986-18068005 None:intergenic 25.0%
!!! TTATTTGCTGTCTTTTTTAG+TGG + chr3_2:18067668-18067687 None:intergenic 25.0%
!! TTGCATTTTATTATCATTGC+TGG + chr3_2:18067895-18067914 None:intergenic 25.0%
!!! TTTTTTCAATGAATTCAGAC+TGG + chr3_2:18067986-18068005 None:intergenic 25.0%
TTCAAAGAGGTGATATAGTA+TGG - chr3_2:18067150-18067169 Msa0397900:CDS 30.0%
TCAAAGAGGTGATATAGTAT+GGG - chr3_2:18067151-18067170 Msa0397900:CDS 30.0%
TGAAAGGGTGATTTATTGAA+GGG + chr3_2:18067311-18067330 None:intergenic 30.0%
TTGAAAGGGTGATTTATTGA+AGG + chr3_2:18067312-18067331 None:intergenic 30.0%
ATAACTATAAACGAGCAGAA+TGG + chr3_2:18067361-18067380 None:intergenic 30.0%
!!! TATTTTGATATGGTGGTGAA+TGG + chr3_2:18067809-18067828 None:intergenic 30.0%
!!! TGTTTGGTATTTTGATATGG+TGG + chr3_2:18067816-18067835 None:intergenic 30.0%
!!! CTCTGTTTGGTATTTTGATA+TGG + chr3_2:18067819-18067838 None:intergenic 30.0%
!!! TTTTGGCTTGTTATTTCTGT+AGG + chr3_2:18068053-18068072 None:intergenic 30.0%
TTCAAAGAGGTGATATAGTA+TGG - chr3_2:18067150-18067169 Msa0397900:CDS 30.0%
TCAAAGAGGTGATATAGTAT+GGG - chr3_2:18067151-18067170 Msa0397900:CDS 30.0%
TGAAAGGGTGATTTATTGAA+GGG + chr3_2:18067311-18067330 None:intergenic 30.0%
TTGAAAGGGTGATTTATTGA+AGG + chr3_2:18067312-18067331 None:intergenic 30.0%
ATAACTATAAACGAGCAGAA+TGG + chr3_2:18067361-18067380 None:intergenic 30.0%
!!! TATTTTGATATGGTGGTGAA+TGG + chr3_2:18067809-18067828 None:intergenic 30.0%
!!! TGTTTGGTATTTTGATATGG+TGG + chr3_2:18067816-18067835 None:intergenic 30.0%
!!! CTCTGTTTGGTATTTTGATA+TGG + chr3_2:18067819-18067838 None:intergenic 30.0%
!!! TTTTGGCTTGTTATTTCTGT+AGG + chr3_2:18068053-18068072 None:intergenic 30.0%
AACTTCAGATTCGACCAAAA+AGG + chr3_2:18067285-18067304 None:intergenic 35.0%
GAAAGGGTGATTTATTGAAG+GGG + chr3_2:18067310-18067329 None:intergenic 35.0%
!!! TGAATGGTGTCGTTTTGAAA+GGG + chr3_2:18067326-18067345 None:intergenic 35.0%
!!! TTGAATGGTGTCGTTTTGAA+AGG + chr3_2:18067327-18067346 None:intergenic 35.0%
ATGGAATGCTTCACATTGAA+TGG + chr3_2:18067342-18067361 None:intergenic 35.0%
! TTTACAGTGTCGCTGTATAA+TGG - chr3_2:18067403-18067422 Msa0397900:intron 35.0%
! TTACAGTGTCGCTGTATAAT+GGG - chr3_2:18067404-18067423 Msa0397900:intron 35.0%
! TATAATGGGACATAACGAAG+AGG - chr3_2:18067418-18067437 Msa0397900:intron 35.0%
! TTGTTGTAGCAGTGTGTTTT+TGG - chr3_2:18067506-18067525 Msa0397900:intron 35.0%
!!! TAGCAGTGTGTTTTTGGTTT+TGG - chr3_2:18067512-18067531 Msa0397900:intron 35.0%
! CTAGTTATGTTGGTTAGATG+TGG + chr3_2:18067716-18067735 None:intergenic 35.0%
CACATCTAACCAACATAACT+AGG - chr3_2:18067714-18067733 Msa0397900:intron 35.0%
!!! CTAGTTTTGCCTAGTTATGT+TGG + chr3_2:18067726-18067745 None:intergenic 35.0%
CAACATAACTAGGCAAAACT+AGG - chr3_2:18067724-18067743 Msa0397900:intron 35.0%
AAAAGACCACTAAGATAGCA+AGG - chr3_2:18067754-18067773 Msa0397900:intron 35.0%
! TGGAGAATGTTGTGTCATTA+TGG + chr3_2:18067789-18067808 None:intergenic 35.0%
!! TTTGTTTAGCTCTCTCTGTT+TGG + chr3_2:18067832-18067851 None:intergenic 35.0%
AATTATAGCAGAACAAGCGT+GGG - chr3_2:18067931-18067950 Msa0397900:CDS 35.0%
!! TCTGTTTTTCAGCCATCTTT+CGG + chr3_2:18067959-18067978 None:intergenic 35.0%
AACTTCAGATTCGACCAAAA+AGG + chr3_2:18067285-18067304 None:intergenic 35.0%
GAAAGGGTGATTTATTGAAG+GGG + chr3_2:18067310-18067329 None:intergenic 35.0%
!!! TGAATGGTGTCGTTTTGAAA+GGG + chr3_2:18067326-18067345 None:intergenic 35.0%
!!! TTGAATGGTGTCGTTTTGAA+AGG + chr3_2:18067327-18067346 None:intergenic 35.0%
ATGGAATGCTTCACATTGAA+TGG + chr3_2:18067342-18067361 None:intergenic 35.0%
! TTTACAGTGTCGCTGTATAA+TGG - chr3_2:18067403-18067422 Msa0397900:intron 35.0%
! TTACAGTGTCGCTGTATAAT+GGG - chr3_2:18067404-18067423 Msa0397900:intron 35.0%
! TATAATGGGACATAACGAAG+AGG - chr3_2:18067418-18067437 Msa0397900:intron 35.0%
! TTGTTGTAGCAGTGTGTTTT+TGG - chr3_2:18067506-18067525 Msa0397900:intron 35.0%
!!! TAGCAGTGTGTTTTTGGTTT+TGG - chr3_2:18067512-18067531 Msa0397900:intron 35.0%
! CTAGTTATGTTGGTTAGATG+TGG + chr3_2:18067716-18067735 None:intergenic 35.0%
CACATCTAACCAACATAACT+AGG - chr3_2:18067714-18067733 Msa0397900:intron 35.0%
!!! CTAGTTTTGCCTAGTTATGT+TGG + chr3_2:18067726-18067745 None:intergenic 35.0%
CAACATAACTAGGCAAAACT+AGG - chr3_2:18067724-18067743 Msa0397900:intron 35.0%
AAAAGACCACTAAGATAGCA+AGG - chr3_2:18067754-18067773 Msa0397900:intron 35.0%
! TGGAGAATGTTGTGTCATTA+TGG + chr3_2:18067789-18067808 None:intergenic 35.0%
!! TTTGTTTAGCTCTCTCTGTT+TGG + chr3_2:18067832-18067851 None:intergenic 35.0%
AATTATAGCAGAACAAGCGT+GGG - chr3_2:18067931-18067950 Msa0397900:CDS 35.0%
!! TCTGTTTTTCAGCCATCTTT+CGG + chr3_2:18067959-18067978 None:intergenic 35.0%
ATGACGAGGAATGTTCAAAG+AGG - chr3_2:18067137-18067156 Msa0397900:CDS 40.0%
TAATTCACTTCCCTCAGAAC+AGG - chr3_2:18067180-18067199 Msa0397900:CDS 40.0%
!!! GAAGAGGAGAGGGTTTTAAA+TGG - chr3_2:18067434-18067453 Msa0397900:intron 40.0%
!!! GAGAGGGTTTTAAATGGTTC+AGG - chr3_2:18067440-18067459 Msa0397900:intron 40.0%
!!! CAGTGTGTTTTTGGTTTTGG+TGG - chr3_2:18067515-18067534 Msa0397900:intron 40.0%
!! TGTTAGCCTTGCTATCTTAG+TGG + chr3_2:18067763-18067782 None:intergenic 40.0%
CACGCTTGTTCTGCTATAAT+TGG + chr3_2:18067932-18067951 None:intergenic 40.0%
CAATTATAGCAGAACAAGCG+TGG - chr3_2:18067930-18067949 Msa0397900:CDS 40.0%
ATGACGAGGAATGTTCAAAG+AGG - chr3_2:18067137-18067156 Msa0397900:CDS 40.0%
TAATTCACTTCCCTCAGAAC+AGG - chr3_2:18067180-18067199 Msa0397900:CDS 40.0%
!!! GAAGAGGAGAGGGTTTTAAA+TGG - chr3_2:18067434-18067453 Msa0397900:intron 40.0%
!!! GAGAGGGTTTTAAATGGTTC+AGG - chr3_2:18067440-18067459 Msa0397900:intron 40.0%
!!! CAGTGTGTTTTTGGTTTTGG+TGG - chr3_2:18067515-18067534 Msa0397900:intron 40.0%
!! TGTTAGCCTTGCTATCTTAG+TGG + chr3_2:18067763-18067782 None:intergenic 40.0%
CACGCTTGTTCTGCTATAAT+TGG + chr3_2:18067932-18067951 None:intergenic 40.0%
CAATTATAGCAGAACAAGCG+TGG - chr3_2:18067930-18067949 Msa0397900:CDS 40.0%
TTGGAAGATGTGAGCAAAGC+TGG + chr3_2:18067209-18067228 None:intergenic 45.0%
CTCACATCTTCCAACGATCT+CGG - chr3_2:18067215-18067234 Msa0397900:CDS 45.0%
! GACCAAAAAGGTGCATTGTG+GGG + chr3_2:18067273-18067292 None:intergenic 45.0%
! CGACCAAAAAGGTGCATTGT+GGG + chr3_2:18067274-18067293 None:intergenic 45.0%
! TCGACCAAAAAGGTGCATTG+TGG + chr3_2:18067275-18067294 None:intergenic 45.0%
! TTCAGGTTACCAGTTTGACC+CGG - chr3_2:18067457-18067476 Msa0397900:intron 45.0%
! TTTGACCCGGTTGGAGTTTT+GGG - chr3_2:18067470-18067489 Msa0397900:intron 45.0%
TGATACCCAAAACTCCAACC+GGG + chr3_2:18067478-18067497 None:intergenic 45.0%
CTGATACCCAAAACTCCAAC+CGG + chr3_2:18067479-18067498 None:intergenic 45.0%
!!! GTGTTTTTGGTTTTGGTGGC+AGG - chr3_2:18067519-18067538 Msa0397900:intron 45.0%
GTGGCTGTATGACAGTTAGT+AGG + chr3_2:18067865-18067884 None:intergenic 45.0%
TCAGATTCAACCTCAGTCGT+AGG + chr3_2:18068020-18068039 None:intergenic 45.0%
TTGGAAGATGTGAGCAAAGC+TGG + chr3_2:18067209-18067228 None:intergenic 45.0%
CTCACATCTTCCAACGATCT+CGG - chr3_2:18067215-18067234 Msa0397900:CDS 45.0%
! GACCAAAAAGGTGCATTGTG+GGG + chr3_2:18067273-18067292 None:intergenic 45.0%
! CGACCAAAAAGGTGCATTGT+GGG + chr3_2:18067274-18067293 None:intergenic 45.0%
! TCGACCAAAAAGGTGCATTG+TGG + chr3_2:18067275-18067294 None:intergenic 45.0%
! TTCAGGTTACCAGTTTGACC+CGG - chr3_2:18067457-18067476 Msa0397900:intron 45.0%
! TTTGACCCGGTTGGAGTTTT+GGG - chr3_2:18067470-18067489 Msa0397900:intron 45.0%
TGATACCCAAAACTCCAACC+GGG + chr3_2:18067478-18067497 None:intergenic 45.0%
CTGATACCCAAAACTCCAAC+CGG + chr3_2:18067479-18067498 None:intergenic 45.0%
!!! GTGTTTTTGGTTTTGGTGGC+AGG - chr3_2:18067519-18067538 Msa0397900:intron 45.0%
GTGGCTGTATGACAGTTAGT+AGG + chr3_2:18067865-18067884 None:intergenic 45.0%
TCAGATTCAACCTCAGTCGT+AGG + chr3_2:18068020-18068039 None:intergenic 45.0%
AAGCTGGAGACCTGTTCTGA+GGG + chr3_2:18067193-18067212 None:intergenic 50.0%
AAAGCTGGAGACCTGTTCTG+AGG + chr3_2:18067194-18067213 None:intergenic 50.0%
! AAGGTGCATTGTGGGGTGTT+GGG + chr3_2:18067266-18067285 None:intergenic 50.0%
! AAAGGTGCATTGTGGGGTGT+TGG + chr3_2:18067267-18067286 None:intergenic 50.0%
! CACCCCACAATGCACCTTTT+TGG - chr3_2:18067268-18067287 Msa0397900:intron 50.0%
TGGGACATAACGAAGAGGAG+AGG - chr3_2:18067423-18067442 Msa0397900:intron 50.0%
GGGACATAACGAAGAGGAGA+GGG - chr3_2:18067424-18067443 Msa0397900:intron 50.0%
AAACTCCAACCGGGTCAAAC+TGG + chr3_2:18067469-18067488 None:intergenic 50.0%
! GTTTGACCCGGTTGGAGTTT+TGG - chr3_2:18067469-18067488 Msa0397900:intron 50.0%
! TATCATTGCTGGCTGCACTG+TGG + chr3_2:18067884-18067903 None:intergenic 50.0%
GATTCAACCTCAGTCGTAGG+AGG + chr3_2:18068017-18068036 None:intergenic 50.0%
AAGCTGGAGACCTGTTCTGA+GGG + chr3_2:18067193-18067212 None:intergenic 50.0%
AAAGCTGGAGACCTGTTCTG+AGG + chr3_2:18067194-18067213 None:intergenic 50.0%
! AAGGTGCATTGTGGGGTGTT+GGG + chr3_2:18067266-18067285 None:intergenic 50.0%
! AAAGGTGCATTGTGGGGTGT+TGG + chr3_2:18067267-18067286 None:intergenic 50.0%
! CACCCCACAATGCACCTTTT+TGG - chr3_2:18067268-18067287 Msa0397900:intron 50.0%
TGGGACATAACGAAGAGGAG+AGG - chr3_2:18067423-18067442 Msa0397900:intron 50.0%
GGGACATAACGAAGAGGAGA+GGG - chr3_2:18067424-18067443 Msa0397900:intron 50.0%
AAACTCCAACCGGGTCAAAC+TGG + chr3_2:18067469-18067488 None:intergenic 50.0%
! GTTTGACCCGGTTGGAGTTT+TGG - chr3_2:18067469-18067488 Msa0397900:intron 50.0%
! TATCATTGCTGGCTGCACTG+TGG + chr3_2:18067884-18067903 None:intergenic 50.0%
GATTCAACCTCAGTCGTAGG+AGG + chr3_2:18068017-18068036 None:intergenic 50.0%
GAGTGAGATGCCGAGATCGT+TGG + chr3_2:18067228-18067247 None:intergenic 55.0%
!! AGGTGCATTGTGGGGTGTTG+GGG + chr3_2:18067265-18067284 None:intergenic 55.0%
GGTTACCAGTTTGACCCGGT+TGG - chr3_2:18067461-18067480 Msa0397900:intron 55.0%
CCTAGCTCAAGACGAGCAAG+AGG + chr3_2:18067565-18067584 None:intergenic 55.0%
CCTCTTGCTCGTCTTGAGCT+AGG - chr3_2:18067562-18067581 Msa0397900:intron 55.0%
CAAGCGTGGGAACCGAAAGA+TGG - chr3_2:18067944-18067963 Msa0397900:CDS 55.0%
GAGCATTCCTCCTACGACTG+AGG - chr3_2:18068007-18068026 Msa0397900:CDS 55.0%
GAGTGAGATGCCGAGATCGT+TGG + chr3_2:18067228-18067247 None:intergenic 55.0%
!! AGGTGCATTGTGGGGTGTTG+GGG + chr3_2:18067265-18067284 None:intergenic 55.0%
GGTTACCAGTTTGACCCGGT+TGG - chr3_2:18067461-18067480 Msa0397900:intron 55.0%
CCTAGCTCAAGACGAGCAAG+AGG + chr3_2:18067565-18067584 None:intergenic 55.0%
CCTCTTGCTCGTCTTGAGCT+AGG - chr3_2:18067562-18067581 Msa0397900:intron 55.0%
CAAGCGTGGGAACCGAAAGA+TGG - chr3_2:18067944-18067963 Msa0397900:CDS 55.0%
GAGCATTCCTCCTACGACTG+AGG - chr3_2:18068007-18068026 Msa0397900:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 18067137 18068079 18067137 ID=Msa0397900;Name=Msa0397900
chr3_2 mRNA 18067137 18068079 18067137 ID=Msa0397900-mRNA-1;Parent=Msa0397900;Name=Msa0397900-mRNA-1;_AED=0.75;_eAED=0.76;_QI=0|0|0|0.33|1|1|3|0|116
chr3_2 exon 18067915 18068079 18067915 ID=Msa0397900-mRNA-1:exon:4046;Parent=Msa0397900-mRNA-1
chr3_2 exon 18067628 18067675 18067628 ID=Msa0397900-mRNA-1:exon:4045;Parent=Msa0397900-mRNA-1
chr3_2 exon 18067137 18067274 18067137 ID=Msa0397900-mRNA-1:exon:4044;Parent=Msa0397900-mRNA-1
chr3_2 CDS 18067915 18068079 18067915 ID=Msa0397900-mRNA-1:cds;Parent=Msa0397900-mRNA-1
chr3_2 CDS 18067628 18067675 18067628 ID=Msa0397900-mRNA-1:cds;Parent=Msa0397900-mRNA-1
chr3_2 CDS 18067137 18067274 18067137 ID=Msa0397900-mRNA-1:cds;Parent=Msa0397900-mRNA-1
Gene Sequence

>Msa0397900

ATGACGAGGAATGTTCAAAGAGGTGATATAGTATGGGTAAGAATAATTCACTTCCCTCAGAACAGGTCTCCAGCTTTGCTCACATCTTCCAACGATCTCGGCATCTCACTCTCTTTCTTCAACAACCCCAACACCCCACAATGCACCTTTTTGGTCGAATCTGAAGCTGCAGTTCTGTTTATTGCGCCTCTTGCTCGTCTTGAGCTAGGTAGTAACAAGCGTGGGAACCGAAAGATGGCTGAAAAACAGAAAGTCCAGTCTGAATTCATTGAAAAAATGAGCATTCCTCCTACGACTGAGGTTGAATCTGATCTGAAAAGACCTACAGAAATAACAAGCCAAAATAATTAA

Protein sequence

>Msa0397900

MTRNVQRGDIVWVRIIHFPQNRSPALLTSSNDLGISLSFFNNPNTPQCTFLVESEAAVLFIAPLARLELGSNKRGNRKMAEKQKVQSEFIEKMSIPPTTEVESDLKRPTEITSQNN*