AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0831060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0831060 MtrunA17_Chr5g0396801 97.977 346 7 0 1 346 1 346 0.0 697
Msa0831060 MtrunA17_Chr8g0347451 80.337 356 60 2 1 346 6 361 0.0 588
Msa0831060 MtrunA17_Chr7g0252401 39.432 317 152 7 29 341 202 482 8.14e-67 218
Msa0831060 MtrunA17_Chr3g0131501 41.368 307 153 8 36 341 203 483 1.21e-65 214
Msa0831060 MtrunA17_Chr3g0131501 30.412 194 130 2 28 221 289 477 7.55e-25 104
Msa0831060 MtrunA17_Chr3g0135861 37.346 324 159 8 36 340 33 331 2.27e-62 202
Msa0831060 MtrunA17_Chr6g0488081 36.335 322 168 6 34 343 15 311 1.61e-59 194
Msa0831060 MtrunA17_Chr8g0385571 38.436 307 162 7 36 341 225 505 1.73e-59 199
Msa0831060 MtrunA17_Chr8g0385571 26.866 201 142 2 21 221 304 499 9.60e-21 93.2
Msa0831060 MtrunA17_Chr5g0436041 38.318 321 170 7 25 338 22 321 6.51e-59 192
Msa0831060 MtrunA17_Chr7g0240341 37.179 312 163 8 38 338 25 314 2.05e-57 189
Msa0831060 MtrunA17_Chr1g0149111 34.983 303 168 6 36 338 137 410 1.94e-52 179
Msa0831060 MtrunA17_Chr1g0149111 30.542 203 130 3 24 225 219 411 2.75e-22 97.1
Msa0831060 MtrunA17_Chr1g0149111 29.000 200 118 6 138 337 136 311 2.14e-18 85.9
Msa0831060 MtrunA17_Chr3g0137941 33.333 303 173 5 36 338 120 393 5.51e-52 177
Msa0831060 MtrunA17_Chr3g0137941 31.527 203 128 3 24 225 202 394 2.16e-24 102
Msa0831060 MtrunA17_Chr2g0296061 32.919 322 179 8 40 338 17 324 1.03e-49 169
Msa0831060 MtrunA17_Chr2g0296061 28.054 221 147 3 15 224 105 324 2.36e-21 93.2
Msa0831060 MtrunA17_Chr4g0063561 32.343 303 176 6 36 338 121 394 1.41e-47 165
Msa0831060 MtrunA17_Chr4g0063561 27.941 204 137 4 25 228 205 398 4.42e-16 78.6
Msa0831060 MtrunA17_Chr4g0067231 30.864 324 185 6 40 338 16 325 2.39e-47 163
Msa0831060 MtrunA17_Chr4g0067231 27.149 221 149 3 15 224 106 325 1.06e-19 88.6
Msa0831060 MtrunA17_Chr7g0242311 32.432 296 159 6 49 339 98 357 6.28e-45 158
Msa0831060 MtrunA17_Chr4g0006851 32.095 296 160 6 49 339 100 359 7.23e-43 152
Msa0831060 MtrunA17_Chr3g0135801 29.012 324 189 9 31 340 27 323 2.00e-33 126
Msa0831060 MtrunA17_Chr6g0488341 31.298 262 147 5 82 343 8 236 6.73e-33 122
Msa0831060 MtrunA17_Chr8g0382201 30.141 355 194 8 5 341 167 485 1.37e-32 126
Msa0831060 MtrunA17_Chr5g0423111 32.881 295 170 8 50 338 33 305 1.07e-31 122
Msa0831060 MtrunA17_Chr5g0423111 29.189 185 119 6 51 229 132 310 7.49e-13 68.9
Msa0831060 MtrunA17_Chr4g0000261 25.836 329 193 8 36 345 64 360 3.23e-31 120
Msa0831060 MtrunA17_Chr6g0463041 30.579 242 116 4 137 343 21 245 5.13e-31 117
Msa0831060 MtrunA17_Chr6g0463041 25.161 155 105 2 72 224 95 240 5.78e-14 70.9
Msa0831060 MtrunA17_Chr8g0372531 29.373 303 183 8 52 343 28 310 9.19e-30 115
Msa0831060 MtrunA17_Chr7g0269701 29.221 308 184 8 40 338 24 306 1.60e-29 115
Msa0831060 MtrunA17_Chr7g0269701 28.846 208 123 5 138 339 19 207 1.90e-16 79.0
Msa0831060 MtrunA17_Chr7g0269701 27.184 206 131 5 32 229 117 311 3.97e-12 66.2
Msa0831060 MtrunA17_Chr8g0366791 31.348 319 183 7 33 339 4 298 2.68e-29 114
Msa0831060 MtrunA17_Chr3g0115801 31.153 321 178 7 36 341 114 406 6.63e-29 115
Msa0831060 MtrunA17_Chr2g0328891 31.611 329 172 11 13 321 41 336 3.74e-28 112
Msa0831060 MtrunA17_Chr1g0195101 28.289 304 187 8 50 346 31 310 1.99e-27 109
Msa0831060 MtrunA17_Chr5g0411411 28.840 319 191 7 33 339 4 298 7.55e-27 107
Msa0831060 MtrunA17_Chr5g0431531 27.832 309 198 6 24 332 25 308 1.15e-26 107
Msa0831060 MtrunA17_Chr8g0393031 27.667 300 166 10 36 316 16 283 2.41e-26 106
Msa0831060 MtrunA17_Chr8g0393031 25.248 202 130 4 147 340 24 212 1.59e-11 64.7
Msa0831060 MtrunA17_Chr3g0088301 28.829 333 182 11 10 321 65 363 2.06e-24 102
Msa0831060 MtrunA17_Chr8g0386871 25.073 343 206 10 7 341 291 590 3.09e-23 100
Msa0831060 MtrunA17_Chr4g0040341 27.950 322 178 12 37 338 74 361 5.79e-23 98.2
Msa0831060 MtrunA17_Chr8g0352031 28.197 305 167 9 37 321 67 339 1.17e-22 97.4
Msa0831060 MtrunA17_Chr4g0004571 33.673 196 115 7 26 218 103 286 7.60e-22 94.0
Msa0831060 MtrunA17_Chr4g0004571 25.733 307 196 9 36 338 14 292 3.30e-19 86.7
Msa0831060 MtrunA17_Chr1g0201361 24.633 341 210 7 6 346 19 312 6.50e-21 91.7
Msa0831060 MtrunA17_Chr1g0201361 29.956 227 136 5 17 235 104 315 1.84e-18 84.7
Msa0831060 MtrunA17_Chr7g0241521 26.962 293 174 7 54 335 33 296 3.98e-20 89.4
Msa0831060 MtrunA17_Chr7g0241521 27.315 216 138 4 129 338 5 207 5.07e-17 80.5
Msa0831060 MtrunA17_Chr4g0008061 25.868 317 191 9 32 335 9 294 1.12e-19 88.2
Msa0831060 MtrunA17_Chr4g0008061 27.500 200 126 4 145 338 19 205 1.05e-14 73.9
Msa0831060 MtrunA17_Chr3g0086811 24.377 361 216 10 1 336 1 329 3.23e-19 87.4
Msa0831060 MtrunA17_Chr3g0103681 26.586 331 186 9 27 338 6 298 5.31e-19 86.3
Msa0831060 MtrunA17_Chr8g0383321 25.731 342 200 9 4 338 11 305 1.45e-18 85.1
Msa0831060 MtrunA17_Chr8g0383321 29.798 198 125 3 32 229 127 310 1.81e-18 85.1
Msa0831060 MtrunA17_Chr1g0196921 29.480 173 107 4 145 316 18 176 2.93e-17 81.3
Msa0831060 MtrunA17_Chr1g0196921 24.752 303 193 10 47 340 23 299 5.40e-15 74.7
Msa0831060 MtrunA17_Chr3g0133111 23.423 333 201 9 35 338 3 310 8.96e-17 80.1
Msa0831060 MtrunA17_Chr8g0385891 29.555 247 152 9 22 262 59 289 1.54e-15 76.6
Msa0831060 MtrunA17_Chr8g0385891 25.888 197 124 5 142 338 75 249 2.27e-14 73.2
Msa0831060 MtrunA17_Chr6g0455381 24.510 306 203 7 38 335 18 303 2.28e-15 75.9
Msa0831060 MtrunA17_Chr6g0461981 28.205 273 161 11 10 262 22 279 4.50e-15 75.5
Msa0831060 MtrunA17_Chr6g0461981 25.253 198 126 5 141 338 64 239 7.88e-15 74.7
Msa0831060 MtrunA17_Chr4g0028531 26.756 299 182 11 39 328 23 293 6.67e-15 74.3
Msa0831060 MtrunA17_Chr6g0488171 33.036 112 66 1 232 343 5 107 7.74e-14 67.0
Msa0831060 MtrunA17_Chr7g0219891 24.671 304 205 7 38 335 22 307 7.96e-14 71.2
Msa0831060 MtrunA17_Chr7g0251981 29.046 241 145 9 30 262 76 298 8.83e-14 71.6
Msa0831060 MtrunA17_Chr7g0251981 26.154 195 122 5 142 336 84 256 1.33e-13 71.2
Msa0831060 MtrunA17_Chr4g0051841 25.311 241 158 8 29 262 22 247 9.92e-14 71.2
Msa0831060 MtrunA17_Chr4g0051841 24.000 200 128 6 141 338 30 207 2.55e-12 67.0
Msa0831060 MtrunA17_Chr5g0407451 26.446 242 148 7 31 264 16 235 3.64e-13 69.3
Msa0831060 MtrunA17_Chr5g0412401 23.754 341 199 9 35 338 3 319 4.01e-13 69.3
Msa0831060 MtrunA17_Chr5g0436571 26.014 296 172 10 74 343 46 320 8.28e-13 68.6
Msa0831060 MtrunA17_Chr2g0331771 30.921 152 93 4 52 198 263 407 1.37e-12 68.6
Msa0831060 MtrunA17_Chr2g0331771 22.659 331 202 8 28 346 146 434 3.41e-11 63.9
Msa0831060 MtrunA17_Chr2g0293621 22.814 263 182 5 79 335 69 316 6.09e-12 65.9
Msa0831060 MtrunA17_Chr4g0046981 23.659 317 208 7 36 341 10 303 9.81e-12 65.1
Msa0831060 MtrunA17_Chr8g0367641 23.145 337 197 11 36 338 4 312 1.11e-11 65.1
Msa0831060 MtrunA17_Chr1g0196161 28.492 179 118 3 44 221 3 172 1.65e-11 62.4
Msa0831060 MtrunA17_Chr1g0163521 30.435 161 99 4 178 338 204 351 1.68e-11 64.7
Msa0831060 MtrunA17_Chr6g0463351 22.807 285 194 7 51 335 80 338 2.10e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0831060 AT4G01100.1 77.119 354 71 3 1 346 1 352 0.0 561
Msa0831060 AT4G01100.2 73.913 368 72 4 1 346 1 366 0.0 550
Msa0831060 AT4G01100.3 80.795 302 56 1 47 346 1 302 0.0 517
Msa0831060 AT4G26180.1 39.875 321 159 7 34 343 17 314 1.21e-64 208
Msa0831060 AT5G51050.1 39.016 305 156 6 38 341 223 498 2.39e-64 212
Msa0831060 AT5G61810.2 38.235 306 156 6 36 341 63 335 3.41e-60 197
Msa0831060 AT5G61810.1 38.562 306 155 6 36 341 206 478 7.11e-59 197
Msa0831060 AT3G53940.1 37.419 310 167 6 36 338 71 360 8.44e-59 194
Msa0831060 AT3G53940.1 33.168 202 120 6 32 229 174 364 1.95e-24 102
Msa0831060 AT2G37890.1 36.556 331 177 7 17 338 26 332 6.89e-58 191
Msa0831060 AT3G55640.1 37.086 302 169 5 38 335 38 322 9.85e-58 190
Msa0831060 AT3G55640.4 37.086 302 169 5 38 335 38 322 9.85e-58 190
Msa0831060 AT4G26180.2 39.262 298 147 7 34 320 17 291 1.54e-57 188
Msa0831060 AT4G26180.2 27.604 192 132 2 144 335 24 208 1.74e-17 82.0
Msa0831060 AT1G14560.1 36.842 323 171 6 34 343 23 325 4.65e-56 186
Msa0831060 AT5G07320.1 37.037 297 152 7 46 341 217 479 1.87e-55 188
Msa0831060 AT4G32400.1 33.993 303 171 5 36 338 112 385 2.87e-50 172
Msa0831060 AT4G32400.1 29.268 205 130 6 26 226 194 387 2.41e-17 82.8
Msa0831060 AT5G48970.1 33.542 319 184 7 40 338 24 334 4.29e-50 171
Msa0831060 AT5G48970.1 24.554 224 154 3 15 224 112 334 9.57e-16 77.8
Msa0831060 AT1G14560.2 36.667 300 157 6 34 320 23 302 7.12e-49 166
Msa0831060 AT1G14560.2 28.022 182 116 4 160 335 44 216 4.78e-14 72.4
Msa0831060 AT3G21390.1 31.746 315 190 6 40 336 21 328 3.00e-46 160
Msa0831060 AT3G21390.1 25.778 225 151 4 15 224 107 330 2.93e-16 79.0
Msa0831060 AT3G21390.2 31.746 315 190 6 40 336 43 350 4.46e-46 160
Msa0831060 AT3G21390.2 25.778 225 151 3 15 224 129 352 2.88e-16 79.3
Msa0831060 AT5G01500.1 33.550 307 159 8 49 346 130 400 6.15e-44 156
Msa0831060 AT3G51870.1 30.619 307 168 8 49 346 102 372 2.37e-42 151
Msa0831060 AT5G48970.2 32.593 270 167 5 76 338 2 263 3.37e-41 145
Msa0831060 AT5G48970.2 24.554 224 154 3 15 224 41 263 7.05e-16 77.0
Msa0831060 AT3G55640.2 36.771 223 129 4 38 256 38 252 6.05e-38 136
Msa0831060 AT3G55640.2 26.872 227 141 5 118 336 15 224 8.37e-16 76.6
Msa0831060 AT3G55640.3 36.771 223 129 4 38 256 38 252 6.05e-38 136
Msa0831060 AT3G55640.3 26.872 227 141 5 118 336 15 224 8.37e-16 76.6
Msa0831060 AT1G14560.3 35.455 220 116 4 137 343 21 227 6.80e-38 135
Msa0831060 AT1G14560.3 30.288 208 116 5 37 224 24 222 3.82e-24 99.4
Msa0831060 AT3G53940.2 35.965 228 128 5 36 256 71 287 3.32e-35 130
Msa0831060 AT3G53940.2 29.108 213 126 4 131 335 63 258 2.65e-19 87.0
Msa0831060 AT5G56450.1 29.907 321 180 8 36 338 32 325 1.00e-34 130
Msa0831060 AT3G20240.1 28.125 320 183 9 36 338 50 339 1.87e-33 127
Msa0831060 AT3G20240.1 30.964 197 118 6 39 230 164 347 1.06e-16 80.5
Msa0831060 AT5G64970.1 31.173 324 182 6 32 341 129 425 9.49e-33 126
Msa0831060 AT5G64970.2 30.476 315 178 6 41 341 14 301 1.02e-31 121
Msa0831060 AT5G66380.1 28.758 306 194 6 40 338 12 300 8.37e-31 119
Msa0831060 AT5G46800.2 29.154 319 187 7 36 339 6 300 4.82e-28 111
Msa0831060 AT5G46800.2 28.780 205 127 4 32 224 102 299 1.42e-14 73.9
Msa0831060 AT5G46800.3 29.154 319 187 7 36 339 6 300 4.82e-28 111
Msa0831060 AT5G46800.3 28.780 205 127 4 32 224 102 299 1.42e-14 73.9
Msa0831060 AT5G46800.1 29.154 319 187 7 36 339 6 300 4.82e-28 111
Msa0831060 AT5G46800.1 28.780 205 127 4 32 224 102 299 1.42e-14 73.9
Msa0831060 AT1G25380.1 29.866 298 168 8 50 334 33 302 1.42e-27 111
Msa0831060 AT1G25380.1 31.122 196 113 5 148 339 28 205 1.63e-14 74.3
Msa0831060 AT1G25380.2 29.866 298 168 8 50 334 33 302 1.42e-27 111
Msa0831060 AT1G25380.2 31.122 196 113 5 148 339 28 205 1.63e-14 74.3
Msa0831060 AT1G78180.1 33.333 321 171 11 36 341 126 418 1.40e-26 109
Msa0831060 AT5G13490.1 26.582 316 180 8 26 321 76 359 4.11e-25 105
Msa0831060 AT5G13490.2 26.582 316 180 8 26 321 76 359 4.11e-25 105
Msa0831060 AT5G64970.3 29.703 303 172 6 32 320 129 404 4.40e-25 105
Msa0831060 AT5G64970.3 27.919 197 118 4 140 336 134 306 9.60e-13 69.3
Msa0831060 AT3G08580.2 26.582 316 180 8 26 321 72 355 5.12e-25 104
Msa0831060 AT3G08580.1 26.582 316 180 8 26 321 72 355 5.12e-25 104
Msa0831060 AT2G47490.1 27.797 295 184 6 50 338 29 300 5.65e-25 103
Msa0831060 AT2G47490.1 28.342 187 112 5 157 339 33 201 3.06e-13 70.1
Msa0831060 AT2G47490.1 27.473 182 123 4 51 229 130 305 1.44e-11 65.1
Msa0831060 AT3G54110.1 28.025 314 188 8 32 335 9 294 5.07e-24 100
Msa0831060 AT3G54110.1 28.643 199 121 4 145 338 22 204 5.89e-18 83.6
Msa0831060 AT5G58970.1 27.812 320 187 9 32 338 10 298 1.39e-23 99.4
Msa0831060 AT4G28390.1 29.180 305 163 11 37 321 82 353 3.03e-23 99.8
Msa0831060 AT4G28390.2 29.180 305 163 11 37 321 82 353 3.03e-23 99.8
Msa0831060 AT2G30160.1 27.389 314 205 6 26 338 30 321 4.90e-23 98.2
Msa0831060 AT2G30160.1 27.230 213 143 4 15 224 118 321 6.08e-12 66.2
Msa0831060 AT1G34065.2 27.052 329 190 10 11 338 49 328 1.06e-21 94.7
Msa0831060 AT1G34065.1 27.052 329 190 10 11 338 56 335 1.18e-21 94.7
Msa0831060 AT4G11440.1 26.433 314 192 10 29 340 329 605 1.78e-21 95.9
Msa0831060 AT4G11440.1 30.151 199 130 3 26 224 414 603 4.24e-16 79.7
Msa0831060 AT4G11440.2 26.433 314 192 10 29 340 329 605 1.78e-21 95.9
Msa0831060 AT4G11440.2 30.151 199 130 3 26 224 414 603 4.24e-16 79.7
Msa0831060 AT1G07030.1 26.129 310 205 6 29 338 31 316 1.68e-20 91.3
Msa0831060 AT4G39460.2 31.280 211 129 4 27 237 130 324 1.92e-20 90.9
Msa0831060 AT4G39460.2 28.115 313 176 8 27 338 47 311 7.18e-19 86.7
Msa0831060 AT4G39460.1 31.280 211 129 4 27 237 130 324 1.92e-20 90.9
Msa0831060 AT4G39460.1 28.115 313 176 8 27 338 47 311 7.18e-19 86.7
Msa0831060 AT4G39460.3 31.280 211 129 4 27 237 130 324 1.92e-20 90.9
Msa0831060 AT4G39460.3 28.115 313 176 8 27 338 47 311 7.18e-19 86.7
Msa0831060 AT5G17400.1 26.897 290 179 9 37 316 13 279 5.41e-20 89.4
Msa0831060 AT1G74240.1 22.067 358 227 8 3 336 5 334 3.53e-19 87.8
Msa0831060 AT1G74240.1 27.586 203 132 4 144 337 39 235 8.27e-12 66.2
Msa0831060 AT1G14140.1 25.000 304 192 8 47 340 25 302 2.28e-17 82.0
Msa0831060 AT3G48850.1 27.715 267 173 8 1 262 36 287 9.21e-16 77.8
Msa0831060 AT3G48850.1 22.907 227 148 6 116 338 44 247 1.39e-13 71.6
Msa0831060 AT5G14040.1 25.758 198 125 5 141 338 83 258 2.38e-15 76.6
Msa0831060 AT5G14040.1 28.814 236 152 8 30 262 76 298 7.14e-12 66.2
Msa0831060 AT4G24570.1 24.036 337 192 7 36 338 4 310 2.51e-15 76.3
Msa0831060 AT2G22500.1 22.124 339 194 9 36 338 4 308 4.92e-15 75.1
Msa0831060 AT5G01340.1 26.115 314 194 11 39 343 18 302 6.86e-15 74.7
Msa0831060 AT1G74240.2 22.330 309 192 7 52 336 2 286 7.68e-15 74.7
Msa0831060 AT1G74240.2 27.749 191 123 4 156 337 3 187 7.35e-11 62.8
Msa0831060 AT1G79900.1 26.236 263 155 8 54 313 32 258 1.13e-14 73.9
Msa0831060 AT1G79900.1 26.699 206 131 6 25 229 102 288 5.34e-11 63.2
Msa0831060 AT5G58970.2 30.348 201 119 5 145 338 20 206 1.56e-14 73.2
Msa0831060 AT5G58970.2 25.000 252 161 8 32 270 10 246 3.69e-11 63.5
Msa0831060 AT2G39970.1 25.839 298 180 11 74 346 46 327 2.02e-14 73.6
Msa0831060 AT5G09470.1 25.758 264 161 8 81 338 98 332 5.23e-13 69.7
Msa0831060 AT1G72820.1 25.641 273 172 6 84 335 73 335 1.47e-12 68.2
Msa0831060 AT1G72820.2 25.641 273 172 6 84 335 73 335 1.47e-12 68.2
Msa0831060 AT4G03115.2 26.562 192 111 4 150 338 47 211 5.46e-12 65.1
Msa0831060 AT4G03115.4 26.562 192 111 4 150 338 47 211 5.65e-12 65.1
Msa0831060 AT4G27940.1 25.088 283 177 7 71 338 145 407 1.06e-11 65.9
Msa0831060 AT4G03115.1 26.562 192 111 4 150 338 47 211 1.27e-11 65.1
Msa0831060 AT4G03115.1 23.006 326 212 9 22 341 20 312 7.60e-11 62.8
Msa0831060 AT5G26200.1 24.172 302 202 7 46 335 48 334 1.43e-11 65.1
Msa0831060 AT4G03115.3 26.562 192 111 4 150 338 79 243 1.66e-11 65.1
Msa0831060 AT4G03115.3 23.006 326 212 9 22 341 52 344 9.35e-11 62.8
Msa0831060 AT5G42130.1 24.735 283 186 7 54 335 134 390 2.96e-11 64.7
Msa0831060 AT2G17270.1 22.930 314 198 11 31 339 17 291 9.02e-11 62.8

Find 111 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAACTTCACTGCTGAATT+TGG 0.104393 5_4:-4086003 None:intergenic
AAGGATTTCGGTACCATTTC+CGG 0.234210 5_4:-4086167 None:intergenic
TCAGCTAACCTTGACTGAAT+TGG 0.287777 5_4:-4087259 None:intergenic
CTTGCATGTGGGGCTGCTGC+TGG 0.296448 5_4:+4087045 Msa0831060:CDS
ATGCTCTTTCAACAGTCTTC+CGG 0.298234 5_4:+4086764 Msa0831060:CDS
TTCAGGGCTTAAAATATATA+TGG 0.317822 5_4:+4085920 Msa0831060:CDS
GTAAGTGATATGAAATTAAT+AGG 0.322620 5_4:-4084236 None:intergenic
TGCTCTTTCAACAGTCTTCC+GGG 0.332019 5_4:+4086765 Msa0831060:CDS
CTCTTCTGCGTCTTGGAGGT+GGG 0.337736 5_4:+4086515 Msa0831060:CDS
ACTGTAAAGTATGAGGGATT+TGG 0.351570 5_4:+4087213 Msa0831060:CDS
ATGCTCTAGCGCCTTCTTCC+CGG 0.363870 5_4:-4086783 None:intergenic
AAAATACAACGGAACAGTTC+AGG 0.368272 5_4:+4085903 Msa0831060:CDS
AGAACTTCACTGCTGAATTT+GGG 0.371459 5_4:-4086002 None:intergenic
CCATTCCATTCAAGCTTCAA+TGG 0.374959 5_4:+4084292 Msa0831060:exon
AAATCACATGTCAAATCTTA+AGG 0.379134 5_4:-4087704 None:intergenic
AAAATTCAACGGTTTATAAG+AGG 0.382869 5_4:-4084262 None:intergenic
TATCGGTTGCAAACCGGAAA+TGG 0.395097 5_4:+4086154 Msa0831060:CDS
GGGGCTGCTGCTGGAACTAT+TGG 0.396252 5_4:+4087054 Msa0831060:CDS
CCTCTTCTGCGTCTTGGAGG+TGG 0.401652 5_4:+4086514 Msa0831060:CDS
CAGTAAAACTGTAAAGTATG+AGG 0.403490 5_4:+4087206 Msa0831060:CDS
CAGCTAACCTTGACTGAATT+GGG 0.406618 5_4:-4087258 None:intergenic
TGGGATAATACGAGCACAAT+TGG 0.409492 5_4:-4085983 None:intergenic
GGCGCTAGAGCATTGTATAA+AGG 0.419300 5_4:+4086793 Msa0831060:CDS
CTCACTCCTCTTCTGCGTCT+TGG 0.425612 5_4:+4086508 Msa0831060:CDS
GCTTCCGTTGTCACTGGTGA+TGG 0.425707 5_4:+4087144 Msa0831060:CDS
GGATGGTTACCTTCAGTCAT+AGG 0.425783 5_4:+4086814 Msa0831060:CDS
AAAAGCGTGATTAGGAGGTT+TGG 0.431176 5_4:-4084381 None:intergenic
GAGATGGTCAAGGACGTTCT+AGG 0.432330 5_4:+4087636 Msa0831060:CDS
AGACGCCATTGAAGCTTGAA+TGG 0.440385 5_4:-4084297 None:intergenic
AGGGGAATGTTTAAAGGAAA+CGG 0.441281 5_4:+4085958 Msa0831060:CDS
AATGAATTATTCCCTCAAAC+AGG 0.445982 5_4:+4084201 None:intergenic
GTCTCCGCAATCGCCGTTGA+AGG 0.449535 5_4:+4084356 Msa0831060:CDS
CTTGGAGGTGGGGCATGTGC+AGG 0.452142 5_4:+4086526 Msa0831060:CDS
ATACTGAGAAAAGCGTGATT+AGG 0.460163 5_4:-4084389 None:intergenic
AAGGAAAGTATTCCTGTTTG+AGG 0.461692 5_4:-4084213 None:intergenic
TCAACAGTCTTCCGGGAAGA+AGG 0.474429 5_4:+4086772 Msa0831060:CDS
TTAACAGTACCTGTACAGTT+AGG 0.476691 5_4:-4086597 None:intergenic
ACTCCTCTTCTGCGTCTTGG+AGG 0.478152 5_4:+4086511 Msa0831060:CDS
GAGATTGAAGATTCTGCTGC+AGG 0.484269 5_4:+4085591 Msa0831060:CDS
CGTCCTCGCACCAGGTCCAT+CGG 0.488129 5_4:-4086574 None:intergenic
GACCACAAGGCTTGCATGTG+GGG 0.493690 5_4:+4087035 Msa0831060:CDS
AGGAGGTTTGGCACCTTCAA+CGG 0.494537 5_4:-4084369 None:intergenic
GTCCTCGCACCAGGTCCATC+GGG 0.496178 5_4:-4086573 None:intergenic
GGCGCTGCTTCCGTTGTCAC+TGG 0.499991 5_4:+4087138 Msa0831060:CDS
CTTGTCGCAGGTGGAGTCGC+CGG 0.500084 5_4:+4084422 Msa0831060:CDS
AGTCTCTATCGGTTGCAAAC+CGG 0.504263 5_4:+4086148 Msa0831060:CDS
AGGAAAGTATTCCTGTTTGA+GGG 0.504900 5_4:-4084212 None:intergenic
TTCTTCTGCGAATGACATCA+AGG 0.504967 5_4:-4087095 None:intergenic
CGTTGTCACTGGTGATGGGA+AGG 0.506085 5_4:+4087149 Msa0831060:CDS
ATGCAGATGGGAGGCTGGAA+AGG 0.510793 5_4:+4087117 Msa0831060:CDS
GAAGAATGCAGATGGGAGGC+TGG 0.511460 5_4:+4087112 Msa0831060:CDS
TGTCACGAAGGCAATGGCTA+TGG 0.511840 5_4:-4087610 None:intergenic
CTTCCGTTGTCACTGGTGAT+GGG 0.512692 5_4:+4087145 Msa0831060:CDS
GTGATATGAAATTAATAGGA+AGG 0.515840 5_4:-4084232 None:intergenic
GGCACCTTCAACGGCGATTG+CGG 0.521482 5_4:-4084360 None:intergenic
AAATACAACGGAACAGTTCA+GGG 0.525612 5_4:+4085904 Msa0831060:CDS
TATATGAATCTCTAAAAGAC+TGG 0.535507 5_4:+4086962 Msa0831060:CDS
CTGAGAAAAGCGTGATTAGG+AGG 0.537877 5_4:-4084386 None:intergenic
ATCTGCAAATCTCTTGTCGC+AGG 0.542029 5_4:+4084410 Msa0831060:CDS
GTGGTATTCTAAGTCTCTAT+CGG 0.546132 5_4:+4086137 Msa0831060:intron
TCTTCTGCGTCTTGGAGGTG+GGG 0.546986 5_4:+4086516 Msa0831060:CDS
ATGGCGTCTCAAGATTCAGC+TGG 0.547242 5_4:+4084311 Msa0831060:CDS
CATTCGCAGAAGAATGCAGA+TGG 0.551827 5_4:+4087104 Msa0831060:CDS
TGCAAATCTCTTGTCGCAGG+TGG 0.557858 5_4:+4084413 Msa0831060:CDS
CGCAACTTACCCGATGGACC+TGG 0.558753 5_4:+4086564 Msa0831060:CDS
CCACCTCCAAGACGCAGAAG+AGG 0.558893 5_4:-4086514 None:intergenic
GGGATTTGGAGCTTTATACA+AGG 0.559538 5_4:+4087227 Msa0831060:CDS
AGTAAAACTGTAAAGTATGA+GGG 0.564308 5_4:+4087207 Msa0831060:CDS
AAATTCAACGGTTTATAAGA+GGG 0.565924 5_4:-4084261 None:intergenic
TTGATTAAAACAAAACCACT+TGG 0.571366 5_4:+4086985 Msa0831060:CDS
CTTACAACTCCTATGACTGA+AGG 0.574400 5_4:-4086823 None:intergenic
AAAATTCCTCTGTATTGATG+AGG 0.577478 5_4:-4086739 None:intergenic
TTGTCTGCAGATACCGTACG+TGG 0.581757 5_4:+4086924 Msa0831060:intron
ACGAAGGCAATGGCTATGGA+AGG 0.582467 5_4:-4087606 None:intergenic
CTCGTATGTCACGAAGGCAA+TGG 0.590817 5_4:-4087616 None:intergenic
TTATAGTTCACGAACAGCTG+TGG 0.595117 5_4:+4085558 Msa0831060:intron
CAGAGCTAAGTGTGACCACA+AGG 0.595535 5_4:+4087022 Msa0831060:CDS
CCATTGAAGCTTGAATGGAA+TGG 0.596620 5_4:-4084292 None:intergenic
AAGTGAAACTCACACTCCAC+CGG 0.598603 5_4:-4084441 None:intergenic
CTAGAGCATTGTATAAAGGA+TGG 0.598668 5_4:+4086797 Msa0831060:CDS
AAATATATATGGAAAACTGA+GGG 0.600536 5_4:+4085931 Msa0831060:CDS
GTTGTCACTGGTGATGGGAA+GGG 0.601889 5_4:+4087150 Msa0831060:CDS
GTCGCAGGTGGAGTCGCCGG+TGG 0.605211 5_4:+4084425 Msa0831060:CDS
AGAATCTTCAATCTCTCTAG+AGG 0.605927 5_4:-4085583 None:intergenic
GGATTTGGAGCTTTATACAA+GGG 0.609484 5_4:+4087228 Msa0831060:CDS
CTTAGTACCCAATTCAGTCA+AGG 0.610354 5_4:+4087251 Msa0831060:CDS
TCTTCTGCGAATGACATCAA+GGG 0.610404 5_4:-4087094 None:intergenic
AATTATTTACATAAAATCAG+TGG 0.611486 5_4:-4087821 None:intergenic
AGCCCCACATGCAAGCCTTG+TGG 0.617387 5_4:-4087037 None:intergenic
TACCCGATGGACCTGGTGCG+AGG 0.618370 5_4:+4086571 Msa0831060:CDS
TGCCTTCGTGACATACGAGA+TGG 0.618477 5_4:+4087620 Msa0831060:CDS
CTTCTGCGAATGACATCAAG+GGG 0.618902 5_4:-4087093 None:intergenic
CGCAGAAGAATGCAGATGGG+AGG 0.620203 5_4:+4087108 Msa0831060:CDS
AAAATATATATGGAAAACTG+AGG 0.621187 5_4:+4085930 Msa0831060:CDS
GAGTCTTGAGCAATTCCAAG+TGG 0.629550 5_4:-4087000 None:intergenic
TGACCACAAGGCTTGCATGT+GGG 0.632902 5_4:+4087034 Msa0831060:CDS
TAATCTTGAGTACACTGGCA+TGG 0.633949 5_4:+4087173 Msa0831060:CDS
AAGGGTAATCTTGAGTACAC+TGG 0.648550 5_4:+4087168 Msa0831060:CDS
AATGTCCGCAACTTACCCGA+TGG 0.649760 5_4:+4086558 Msa0831060:CDS
CTTCCCATCACCAGTGACAA+CGG 0.650735 5_4:-4087148 None:intergenic
GACCATCTCGTATGTCACGA+AGG 0.657725 5_4:-4087622 None:intergenic
CGTGACATACGAGATGGTCA+AGG 0.657933 5_4:+4087626 Msa0831060:CDS
CAGGTCCATCGGGTAAGTTG+CGG 0.663278 5_4:-4086563 None:intergenic
CGAAGGCAATGGCTATGGAA+GGG 0.663950 5_4:-4087605 None:intergenic
GCATCTCCTCATCAATACAG+AGG 0.672044 5_4:+4086733 Msa0831060:CDS
TGGAACTATTGGCCAGACAG+TGG 0.674835 5_4:+4087065 Msa0831060:CDS
CGTCACAATGTAAAATACAA+CGG 0.675370 5_4:+4085892 Msa0831060:CDS
ATTCGCAGAAGAATGCAGAT+GGG 0.675451 5_4:+4087105 Msa0831060:CDS
GTGACCACAAGGCTTGCATG+TGG 0.679869 5_4:+4087033 Msa0831060:CDS
TGTCTGCAGATACCGTACGT+GGG 0.721600 5_4:+4086925 Msa0831060:intron
TTCTGCGAATGACATCAAGG+GGG 0.801026 5_4:-4087092 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTTTTATCTGAATA+TGG + chr5_4:4086325-4086344 Msa0831060:intron 10.0%
!! ATAAAAATAAAAATAAAGTG+TGG - chr5_4:4086464-4086483 None:intergenic 10.0%
!! ATTTAATCTTCTGATTATTT+AGG + chr5_4:4084611-4084630 Msa0831060:intron 15.0%
!! TACAATATAATATAATAAGG+CGG - chr5_4:4084685-4084704 None:intergenic 15.0%
!!! GAGCTATTAATTATTTATTT+TGG + chr5_4:4084773-4084792 Msa0831060:intron 15.0%
!! ATAAAAAAAGTTCAAAATGT+AGG - chr5_4:4084909-4084928 None:intergenic 15.0%
!!! ATTAATATTTTCTCTCTATT+TGG + chr5_4:4084927-4084946 Msa0831060:intron 15.0%
!! ATTGTATAAAAAGAGTAAAT+TGG - chr5_4:4085175-4085194 None:intergenic 15.0%
!!! CATTTTTATATGCTTAAAAT+AGG + chr5_4:4085498-4085517 Msa0831060:intron 15.0%
!! GATATTTAATGTATTAACAT+AGG + chr5_4:4085645-4085664 Msa0831060:intron 15.0%
!!! TTTTTTTTTCCATTAATCTT+TGG + chr5_4:4086065-4086084 Msa0831060:intron 15.0%
!! GATGTATAAAAGATTATTAA+GGG - chr5_4:4086195-4086214 None:intergenic 15.0%
!! AGATGTATAAAAGATTATTA+AGG - chr5_4:4086196-4086215 None:intergenic 15.0%
!! ATAGAATATTTACTTTATCA+TGG + chr5_4:4086244-4086263 Msa0831060:intron 15.0%
!!! TTTTTATTCATATTATTGTG+AGG + chr5_4:4086397-4086416 Msa0831060:intron 15.0%
!!! TTTTATTCATATTATTGTGA+GGG + chr5_4:4086398-4086417 Msa0831060:intron 15.0%
!! ATTATTGTTTGTAGAATTTA+AGG + chr5_4:4086636-4086655 Msa0831060:intron 15.0%
!! TGAAAATGAATTAAACTTAA+TGG - chr5_4:4086691-4086710 None:intergenic 15.0%
!!! TAAGTTTAATTCATTTTCAA+TGG + chr5_4:4086692-4086711 Msa0831060:intron 15.0%
!! ATGATTTAAATCATTTATCT+AGG + chr5_4:4087336-4087355 Msa0831060:intron 15.0%
!! AAATAGTAATAGTTAAAAAG+TGG + chr5_4:4087526-4087545 Msa0831060:intron 15.0%
!! AATTATTTACATAAAATCAG+TGG - chr5_4:4087824-4087843 None:intergenic 15.0%
!! GTAAGTGATATGAAATTAAT+AGG - chr5_4:4084239-4084258 None:intergenic 20.0%
!! AAATACAGAAACAGAAAAAT+GGG - chr5_4:4084652-4084671 None:intergenic 20.0%
!! ACAATATAATATAATAAGGC+GGG - chr5_4:4084684-4084703 None:intergenic 20.0%
!!! CTCAAACTTTAAATGATTTT+TGG + chr5_4:4084852-4084871 Msa0831060:intron 20.0%
!! TTTGTGAATTATGTTTCATT+TGG + chr5_4:4085351-4085370 Msa0831060:intron 20.0%
!!! AACCTTTAAAGTTTAAACAT+TGG - chr5_4:4085693-4085712 None:intergenic 20.0%
!!! TAAGATCATTTTTAAAATGC+AGG + chr5_4:4085861-4085880 Msa0831060:intron 20.0%
!! AAAATATATATGGAAAACTG+AGG + chr5_4:4085930-4085949 Msa0831060:CDS 20.0%
!! AAATATATATGGAAAACTGA+GGG + chr5_4:4085931-4085950 Msa0831060:CDS 20.0%
!! ACAAATTTACCAAAGATTAA+TGG - chr5_4:4086077-4086096 None:intergenic 20.0%
!!! GATTATTTTATTCTGTCAAT+TGG + chr5_4:4086278-4086297 Msa0831060:intron 20.0%
!! TTTATTCATATTATTGTGAG+GGG + chr5_4:4086399-4086418 Msa0831060:intron 20.0%
!! AGCAATTAATTAATGCAAAT+GGG - chr5_4:4087297-4087316 None:intergenic 20.0%
!! AAGCAATTAATTAATGCAAA+TGG - chr5_4:4087298-4087317 None:intergenic 20.0%
!! ATTTAAATCATTTATCTAGG+TGG + chr5_4:4087339-4087358 Msa0831060:intron 20.0%
!! TTTAAATCATTTATCTAGGT+GGG + chr5_4:4087340-4087359 Msa0831060:intron 20.0%
!! CTAAAATTCTAAATTTCTCT+AGG - chr5_4:4087403-4087422 None:intergenic 20.0%
!!! ATTTAGAATTTTAGATACAG+TGG + chr5_4:4087409-4087428 Msa0831060:intron 20.0%
!! GAACACAAAATTCAATTTAT+AGG + chr5_4:4087500-4087519 Msa0831060:intron 20.0%
!! TAGTTAAAAAGTGGTTTATA+TGG + chr5_4:4087535-4087554 Msa0831060:intron 20.0%
! GTGATATGAAATTAATAGGA+AGG - chr5_4:4084235-4084254 None:intergenic 25.0%
! AAATTCAACGGTTTATAAGA+GGG - chr5_4:4084264-4084283 None:intergenic 25.0%
! AAAATTCAACGGTTTATAAG+AGG - chr5_4:4084265-4084284 None:intergenic 25.0%
!! TATAAACCGTTGAATTTTTG+AGG + chr5_4:4084267-4084286 Msa0831060:five_prime_UTR 25.0%
! GAAATACAGAAACAGAAAAA+TGG - chr5_4:4084653-4084672 None:intergenic 25.0%
! AAAAAAAATAGATTCACACG+TGG - chr5_4:4084742-4084761 None:intergenic 25.0%
! GTTCAAAATGTAGGTTTATA+TGG - chr5_4:4084900-4084919 None:intergenic 25.0%
!!! GTTTTGTTATAGAAATCACT+TGG - chr5_4:4085090-4085109 None:intergenic 25.0%
! AGTGATTTCTATAACAAAAC+TGG + chr5_4:4085090-4085109 Msa0831060:intron 25.0%
! TTGTATACAATCAATCTAAG+TGG + chr5_4:4085245-4085264 Msa0831060:intron 25.0%
!!! TAGTTTTGATTTTCATCTCA+AGG - chr5_4:4085303-4085322 None:intergenic 25.0%
!! TTATGATTTTGCATTGTGAT+AGG + chr5_4:4085469-4085488 Msa0831060:intron 25.0%
! ACATGTTACTATAAGCTTAA+AGG - chr5_4:4085623-4085642 None:intergenic 25.0%
! CTCCAATGTTTAAACTTTAA+AGG + chr5_4:4085688-4085707 Msa0831060:intron 25.0%
!! AAAGGTTTATTGTTGATTAC+TGG + chr5_4:4085706-4085725 Msa0831060:intron 25.0%
! AATGCATTTAAGAAAATGTG+TGG + chr5_4:4085783-4085802 Msa0831060:intron 25.0%
! ACTGAACAACAAGTAATATA+AGG - chr5_4:4085838-4085857 None:intergenic 25.0%
! TTCAGGGCTTAAAATATATA+TGG + chr5_4:4085920-4085939 Msa0831060:CDS 25.0%
! TAAAAGATTATTAAGGGAGA+AGG - chr5_4:4086189-4086208 None:intergenic 25.0%
! ATATTTACTTTATCATGGTG+TGG + chr5_4:4086249-4086268 Msa0831060:intron 25.0%
! TGAATAAAAATGCAGATTGA+AGG - chr5_4:4086387-4086406 None:intergenic 25.0%
! TAAAAATAAAGTGTGGACAA+TGG - chr5_4:4086457-4086476 None:intergenic 25.0%
! GAAACATGAAATTAAAAGCT+TGG - chr5_4:4086890-4086909 None:intergenic 25.0%
!! TATATGAATCTCTAAAAGAC+TGG + chr5_4:4086962-4086981 Msa0831060:CDS 25.0%
! TTGATTAAAACAAAACCACT+TGG + chr5_4:4086985-4087004 Msa0831060:CDS 25.0%
! AGTAAAACTGTAAAGTATGA+GGG + chr5_4:4087207-4087226 Msa0831060:CDS 25.0%
! TTCTATGACTAAGACAAAAT+TGG - chr5_4:4087483-4087502 None:intergenic 25.0%
! AAATCACATGTCAAATCTTA+AGG - chr5_4:4087707-4087726 None:intergenic 25.0%
ATGGAACCTCAAAAATTCAA+CGG - chr5_4:4084276-4084295 None:intergenic 30.0%
AAGAGAACGGTATAAGTATA+AGG - chr5_4:4084528-4084547 None:intergenic 30.0%
CAAACTAAAGAACTTATCGT+TGG - chr5_4:4084555-4084574 None:intergenic 30.0%
! GACATTCAAGAGTTGTTATT+TGG - chr5_4:4085031-4085050 None:intergenic 30.0%
! TTCAGACCAAAGTTGTTATT+TGG - chr5_4:4085119-4085138 None:intergenic 30.0%
TATCTATGTACATATCGCTT+TGG - chr5_4:4085220-4085239 None:intergenic 30.0%
CATTTAAGAAAATGTGTGGA+TGG + chr5_4:4085787-4085806 Msa0831060:intron 30.0%
CGTCACAATGTAAAATACAA+CGG + chr5_4:4085892-4085911 Msa0831060:CDS 30.0%
AGTATAAAACAGTGATCAGA+AGG - chr5_4:4086111-4086130 None:intergenic 30.0%
! ACTGTTTTATACTAATGCAG+TGG + chr5_4:4086118-4086137 Msa0831060:intron 30.0%
TTATTAAGGGAGAAGGATTT+CGG - chr5_4:4086182-4086201 None:intergenic 30.0%
! TCTATCTGCTTTATTAGAGT+TGG - chr5_4:4086229-4086248 None:intergenic 30.0%
!! CATTGACTTGTTATCTTTGT+TGG + chr5_4:4086665-4086684 Msa0831060:intron 30.0%
! AAAATTCCTCTGTATTGATG+AGG - chr5_4:4086742-4086761 None:intergenic 30.0%
CATGAAATTAAAAGCTTGGT+TGG - chr5_4:4086886-4086905 None:intergenic 30.0%
GAAAAATTTAAACCCACGTA+CGG - chr5_4:4086940-4086959 None:intergenic 30.0%
CAGTAAAACTGTAAAGTATG+AGG + chr5_4:4087206-4087225 Msa0831060:CDS 30.0%
ATTGCAAGGAAACAAAAACA+CGG + chr5_4:4087440-4087459 Msa0831060:intron 30.0%
CAATTGCAATTCTTCTCTAA+TGG + chr5_4:4087571-4087590 Msa0831060:intron 30.0%
AATTCTTCTCTAATGGTTTC+AGG + chr5_4:4087578-4087597 Msa0831060:intron 30.0%
! ATACTGAGAAAAGCGTGATT+AGG - chr5_4:4084392-4084411 None:intergenic 35.0%
TATCGTTGGATTGAAGAGAA+CGG - chr5_4:4084541-4084560 None:intergenic 35.0%
AAAATGGGATTCACATGGAA+AGG - chr5_4:4084637-4084656 None:intergenic 35.0%
ACAGAAAAATGGGATTCACA+TGG - chr5_4:4084642-4084661 None:intergenic 35.0%
!! TCTGTTTCTGTATTTCCTAC+CGG + chr5_4:4084657-4084676 Msa0831060:intron 35.0%
! TCTCTATTTGGATGATGATG+AGG + chr5_4:4084939-4084958 Msa0831060:intron 35.0%
! AGTTCTGTGTGTATTTTACG+CGG + chr5_4:4084973-4084992 Msa0831060:intron 35.0%
AATGTCGTCTTGATTACCAA+AGG + chr5_4:4085045-4085064 Msa0831060:intron 35.0%
! AAAGTTTGCTCTTCAACCTT+TGG - chr5_4:4085064-4085083 None:intergenic 35.0%
! ATCTATCTCTATTGATGGAC+TGG - chr5_4:4085399-4085418 None:intergenic 35.0%
GTCCATCAATAGAGATAGAT+TGG + chr5_4:4085399-4085418 Msa0831060:intron 35.0%
AGCCAATCTATCTCTATTGA+TGG - chr5_4:4085404-4085423 None:intergenic 35.0%
! TTCAAAGATGCACTGTGTTT+TGG + chr5_4:4085445-4085464 Msa0831060:intron 35.0%
! TCAAAGATGCACTGTGTTTT+GGG + chr5_4:4085446-4085465 Msa0831060:intron 35.0%
AGAATCTTCAATCTCTCTAG+AGG - chr5_4:4085586-4085605 None:intergenic 35.0%
! TATTGTTGATTACTGGACTC+AGG + chr5_4:4085713-4085732 Msa0831060:intron 35.0%
TAAGAAAATGTGTGGATGGA+TGG + chr5_4:4085791-4085810 Msa0831060:intron 35.0%
AAAATACAACGGAACAGTTC+AGG + chr5_4:4085903-4085922 Msa0831060:CDS 35.0%
AAATACAACGGAACAGTTCA+GGG + chr5_4:4085904-4085923 Msa0831060:CDS 35.0%
! TATGGAAAACTGAGGGTTTT+AGG + chr5_4:4085938-4085957 Msa0831060:CDS 35.0%
! ATGGAAAACTGAGGGTTTTA+GGG + chr5_4:4085939-4085958 Msa0831060:CDS 35.0%
!!! GGTTTTAGGGGAATGTTTAA+AGG + chr5_4:4085952-4085971 Msa0831060:CDS 35.0%
AGGGGAATGTTTAAAGGAAA+CGG + chr5_4:4085958-4085977 Msa0831060:CDS 35.0%
AGAACTTCACTGCTGAATTT+GGG - chr5_4:4086005-4086024 None:intergenic 35.0%
AAGAACTTCACTGCTGAATT+TGG - chr5_4:4086006-4086025 None:intergenic 35.0%
GTGGTATTCTAAGTCTCTAT+CGG + chr5_4:4086137-4086156 Msa0831060:intron 35.0%
TCGAGCTAACAACTTGAATA+TGG - chr5_4:4086359-4086378 None:intergenic 35.0%
TTAACAGTACCTGTACAGTT+AGG - chr5_4:4086600-4086619 None:intergenic 35.0%
!! CTAGAGCATTGTATAAAGGA+TGG + chr5_4:4086797-4086816 Msa0831060:CDS 35.0%
ACTGTAAAGTATGAGGGATT+TGG + chr5_4:4087213-4087232 Msa0831060:CDS 35.0%
! GGATTTGGAGCTTTATACAA+GGG + chr5_4:4087228-4087247 Msa0831060:CDS 35.0%
AATCATTTATCTAGGTGGGT+AGG + chr5_4:4087344-4087363 Msa0831060:intron 35.0%
CAGTGGTATATGAAATTGCA+AGG + chr5_4:4087426-4087445 Msa0831060:intron 35.0%
TTGCAAGGAAACAAAAACAC+GGG + chr5_4:4087441-4087460 Msa0831060:intron 35.0%
CCATTGAAGCTTGAATGGAA+TGG - chr5_4:4084295-4084314 None:intergenic 40.0%
CCATTCCATTCAAGCTTCAA+TGG + chr5_4:4084292-4084311 Msa0831060:exon 40.0%
! AAAAGCGTGATTAGGAGGTT+TGG - chr5_4:4084384-4084403 None:intergenic 40.0%
!! CTGTTTCTGTATTTCCTACC+GGG + chr5_4:4084658-4084677 Msa0831060:intron 40.0%
ATAATATAATAAGGCGGGCC+CGG - chr5_4:4084679-4084698 None:intergenic 40.0%
! TGTATTTTACGCGGTGTCTT+TGG + chr5_4:4084982-4085001 Msa0831060:intron 40.0%
TGGCGTCCAAATAACAACTT+TGG + chr5_4:4085110-4085129 Msa0831060:intron 40.0%
! GTGCATCTTTGAAAATCAGC+TGG - chr5_4:4085438-4085457 None:intergenic 40.0%
TTATAGTTCACGAACAGCTG+TGG + chr5_4:4085558-4085577 Msa0831060:intron 40.0%
!! TGGAAAACTGAGGGTTTTAG+GGG + chr5_4:4085940-4085959 Msa0831060:CDS 40.0%
TGGGATAATACGAGCACAAT+TGG - chr5_4:4085986-4086005 None:intergenic 40.0%
AAACAGTGATCAGAAGGACA+TGG - chr5_4:4086105-4086124 None:intergenic 40.0%
AAGGATTTCGGTACCATTTC+CGG - chr5_4:4086170-4086189 None:intergenic 40.0%
TACCTACCTTCCAAACTTCA+AGG + chr5_4:4086425-4086444 Msa0831060:intron 40.0%
! ACATAGCCTTGAAGTTTGGA+AGG - chr5_4:4086434-4086453 None:intergenic 40.0%
! ATGGACATAGCCTTGAAGTT+TGG - chr5_4:4086438-4086457 None:intergenic 40.0%
ATGCTCTTTCAACAGTCTTC+CGG + chr5_4:4086764-4086783 Msa0831060:CDS 40.0%
CTTACAACTCCTATGACTGA+AGG - chr5_4:4086826-4086845 None:intergenic 40.0%
TCTTCTGCGAATGACATCAA+GGG - chr5_4:4087097-4087116 None:intergenic 40.0%
TTCTTCTGCGAATGACATCA+AGG - chr5_4:4087098-4087117 None:intergenic 40.0%
ATTCGCAGAAGAATGCAGAT+GGG + chr5_4:4087105-4087124 Msa0831060:CDS 40.0%
AAGGGTAATCTTGAGTACAC+TGG + chr5_4:4087168-4087187 Msa0831060:CDS 40.0%
TAATCTTGAGTACACTGGCA+TGG + chr5_4:4087173-4087192 Msa0831060:CDS 40.0%
! GGGATTTGGAGCTTTATACA+AGG + chr5_4:4087227-4087246 Msa0831060:CDS 40.0%
CTTAGTACCCAATTCAGTCA+AGG + chr5_4:4087251-4087270 Msa0831060:CDS 40.0%
CAGCTAACCTTGACTGAATT+GGG - chr5_4:4087261-4087280 None:intergenic 40.0%
TCAGCTAACCTTGACTGAAT+TGG - chr5_4:4087262-4087281 None:intergenic 40.0%
TGCAAGGAAACAAAAACACG+GGG + chr5_4:4087442-4087461 Msa0831060:intron 40.0%
TCTTCTCTAATGGTTTCAGG+TGG + chr5_4:4087581-4087600 Msa0831060:intron 40.0%
AGACGCCATTGAAGCTTGAA+TGG - chr5_4:4084300-4084319 None:intergenic 45.0%
! CTGAGAAAAGCGTGATTAGG+AGG - chr5_4:4084389-4084408 None:intergenic 45.0%
ATCTGCAAATCTCTTGTCGC+AGG + chr5_4:4084410-4084429 Msa0831060:CDS 45.0%
AAGTGAAACTCACACTCCAC+CGG - chr5_4:4084444-4084463 None:intergenic 45.0%
!!! GACTGGTTTAGACGTGCTTT+TGG - chr5_4:4085382-4085401 None:intergenic 45.0%
GAGATTGAAGATTCTGCTGC+AGG + chr5_4:4085591-4085610 Msa0831060:CDS 45.0%
AAAATGTGTGGATGGATGGC+AGG + chr5_4:4085795-4085814 Msa0831060:intron 45.0%
AAATGTGTGGATGGATGGCA+GGG + chr5_4:4085796-4085815 Msa0831060:intron 45.0%
! AGTCTCTATCGGTTGCAAAC+CGG + chr5_4:4086148-4086167 Msa0831060:CDS 45.0%
! TATCGGTTGCAAACCGGAAA+TGG + chr5_4:4086154-4086173 Msa0831060:CDS 45.0%
AGCCTTGAAGTTTGGAAGGT+AGG - chr5_4:4086430-4086449 None:intergenic 45.0%
GCATCTCCTCATCAATACAG+AGG + chr5_4:4086733-4086752 Msa0831060:CDS 45.0%
TGCTCTTTCAACAGTCTTCC+GGG + chr5_4:4086765-4086784 Msa0831060:CDS 45.0%
!! GGCGCTAGAGCATTGTATAA+AGG + chr5_4:4086793-4086812 Msa0831060:CDS 45.0%
GGATGGTTACCTTCAGTCAT+AGG + chr5_4:4086814-4086833 Msa0831060:CDS 45.0%
GAGTCTTGAGCAATTCCAAG+TGG - chr5_4:4087003-4087022 None:intergenic 45.0%
TTCTGCGAATGACATCAAGG+GGG - chr5_4:4087095-4087114 None:intergenic 45.0%
CTTCTGCGAATGACATCAAG+GGG - chr5_4:4087096-4087115 None:intergenic 45.0%
CATTCGCAGAAGAATGCAGA+TGG + chr5_4:4087104-4087123 Msa0831060:CDS 45.0%
!! TAATACAATATAATATAATA+AGG - chr5_4:4084688-4084707 None:intergenic 5.0%
ATGGCGTCTCAAGATTCAGC+TGG + chr5_4:4084311-4084330 Msa0831060:CDS 50.0%
AGGAGGTTTGGCACCTTCAA+CGG - chr5_4:4084372-4084391 None:intergenic 50.0%
TGCAAATCTCTTGTCGCAGG+TGG + chr5_4:4084413-4084432 Msa0831060:CDS 50.0%
AATGTCCGCAACTTACCCGA+TGG + chr5_4:4086558-4086577 Msa0831060:CDS 50.0%
TCAACAGTCTTCCGGGAAGA+AGG + chr5_4:4086772-4086791 Msa0831060:CDS 50.0%
TTGTCTGCAGATACCGTACG+TGG + chr5_4:4086924-4086943 Msa0831060:intron 50.0%
TGTCTGCAGATACCGTACGT+GGG + chr5_4:4086925-4086944 Msa0831060:intron 50.0%
CAGAGCTAAGTGTGACCACA+AGG + chr5_4:4087022-4087041 Msa0831060:CDS 50.0%
TGACCACAAGGCTTGCATGT+GGG + chr5_4:4087034-4087053 Msa0831060:CDS 50.0%
TGGAACTATTGGCCAGACAG+TGG + chr5_4:4087065-4087084 Msa0831060:CDS 50.0%
CTTCCGTTGTCACTGGTGAT+GGG + chr5_4:4087145-4087164 Msa0831060:CDS 50.0%
CTTCCCATCACCAGTGACAA+CGG - chr5_4:4087151-4087170 None:intergenic 50.0%
!! GTTGTCACTGGTGATGGGAA+GGG + chr5_4:4087150-4087169 Msa0831060:CDS 50.0%
CGAAGGCAATGGCTATGGAA+GGG - chr5_4:4087608-4087627 None:intergenic 50.0%
ACGAAGGCAATGGCTATGGA+AGG - chr5_4:4087609-4087628 None:intergenic 50.0%
TGTCACGAAGGCAATGGCTA+TGG - chr5_4:4087613-4087632 None:intergenic 50.0%
CTCGTATGTCACGAAGGCAA+TGG - chr5_4:4087619-4087638 None:intergenic 50.0%
TGCCTTCGTGACATACGAGA+TGG + chr5_4:4087620-4087639 Msa0831060:CDS 50.0%
GACCATCTCGTATGTCACGA+AGG - chr5_4:4087625-4087644 None:intergenic 50.0%
CGTGACATACGAGATGGTCA+AGG + chr5_4:4087626-4087645 Msa0831060:CDS 50.0%
GAGATGGTCAAGGACGTTCT+AGG + chr5_4:4087636-4087655 Msa0831060:CDS 50.0%
TATAATAAGGCGGGCCCGGT+AGG - chr5_4:4084675-4084694 None:intergenic 55.0%
CTCACTCCTCTTCTGCGTCT+TGG + chr5_4:4086508-4086527 Msa0831060:CDS 55.0%
ACTCCTCTTCTGCGTCTTGG+AGG + chr5_4:4086511-4086530 Msa0831060:CDS 55.0%
CTCTTCTGCGTCTTGGAGGT+GGG + chr5_4:4086515-4086534 Msa0831060:CDS 55.0%
TCTTCTGCGTCTTGGAGGTG+GGG + chr5_4:4086516-4086535 Msa0831060:CDS 55.0%
CAGGTCCATCGGGTAAGTTG+CGG - chr5_4:4086566-4086585 None:intergenic 55.0%
ATGCTCTAGCGCCTTCTTCC+CGG - chr5_4:4086786-4086805 None:intergenic 55.0%
GTGACCACAAGGCTTGCATG+TGG + chr5_4:4087033-4087052 Msa0831060:CDS 55.0%
GACCACAAGGCTTGCATGTG+GGG + chr5_4:4087035-4087054 Msa0831060:CDS 55.0%
CGCAGAAGAATGCAGATGGG+AGG + chr5_4:4087108-4087127 Msa0831060:CDS 55.0%
GAAGAATGCAGATGGGAGGC+TGG + chr5_4:4087112-4087131 Msa0831060:CDS 55.0%
ATGCAGATGGGAGGCTGGAA+AGG + chr5_4:4087117-4087136 Msa0831060:CDS 55.0%
GCTTCCGTTGTCACTGGTGA+TGG + chr5_4:4087144-4087163 Msa0831060:CDS 55.0%
!! CGTTGTCACTGGTGATGGGA+AGG + chr5_4:4087149-4087168 Msa0831060:CDS 55.0%
GTCTCCGCAATCGCCGTTGA+AGG + chr5_4:4084356-4084375 Msa0831060:CDS 60.0%
GGCACCTTCAACGGCGATTG+CGG - chr5_4:4084363-4084382 None:intergenic 60.0%
CCACCTCCAAGACGCAGAAG+AGG - chr5_4:4086517-4086536 None:intergenic 60.0%
CCTCTTCTGCGTCTTGGAGG+TGG + chr5_4:4086514-4086533 Msa0831060:CDS 60.0%
CGCAACTTACCCGATGGACC+TGG + chr5_4:4086564-4086583 Msa0831060:CDS 60.0%
GCGAGGACGCCTAACTGTAC+AGG + chr5_4:4086588-4086607 Msa0831060:CDS 60.0%
AGCCCCACATGCAAGCCTTG+TGG - chr5_4:4087040-4087059 None:intergenic 60.0%
GGGGCTGCTGCTGGAACTAT+TGG + chr5_4:4087054-4087073 Msa0831060:CDS 60.0%
! CAAGGGGGTATGCCACTGTC+TGG - chr5_4:4087080-4087099 None:intergenic 60.0%
CTTGTCGCAGGTGGAGTCGC+CGG + chr5_4:4084422-4084441 Msa0831060:CDS 65.0%
CTTGGAGGTGGGGCATGTGC+AGG + chr5_4:4086526-4086545 Msa0831060:CDS 65.0%
TACCCGATGGACCTGGTGCG+AGG + chr5_4:4086571-4086590 Msa0831060:CDS 65.0%
GTCCTCGCACCAGGTCCATC+GGG - chr5_4:4086576-4086595 None:intergenic 65.0%
CGTCCTCGCACCAGGTCCAT+CGG - chr5_4:4086577-4086596 None:intergenic 65.0%
CAGTTAGGCGTCCTCGCACC+AGG - chr5_4:4086585-4086604 None:intergenic 65.0%
CTTGCATGTGGGGCTGCTGC+TGG + chr5_4:4087045-4087064 Msa0831060:CDS 65.0%
GGCGCTGCTTCCGTTGTCAC+TGG + chr5_4:4087138-4087157 Msa0831060:CDS 65.0%
GTCGCAGGTGGAGTCGCCGG+TGG + chr5_4:4084425-4084444 Msa0831060:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 4084214 4087858 4084214 ID=Msa0831060;Name=Msa0831060
chr5_4 mRNA 4084214 4087858 4084214 ID=Msa0831060-mRNA-1;Parent=Msa0831060;Name=Msa0831060-mRNA-1;_AED=0.17;_eAED=0.17;_QI=97|1|1|1|1|1|8|175|346
chr5_4 exon 4084214 4084450 4084214 ID=Msa0831060-mRNA-1:exon:1631;Parent=Msa0831060-mRNA-1
chr5_4 exon 4085564 4085612 4085564 ID=Msa0831060-mRNA-1:exon:1632;Parent=Msa0831060-mRNA-1
chr5_4 exon 4085883 4086046 4085883 ID=Msa0831060-mRNA-1:exon:1633;Parent=Msa0831060-mRNA-1
chr5_4 exon 4086138 4086175 4086138 ID=Msa0831060-mRNA-1:exon:1634;Parent=Msa0831060-mRNA-1
chr5_4 exon 4086497 4086609 4086497 ID=Msa0831060-mRNA-1:exon:1635;Parent=Msa0831060-mRNA-1
chr5_4 exon 4086727 4086840 4086727 ID=Msa0831060-mRNA-1:exon:1636;Parent=Msa0831060-mRNA-1
chr5_4 exon 4086934 4087272 4086934 ID=Msa0831060-mRNA-1:exon:1637;Parent=Msa0831060-mRNA-1
chr5_4 exon 4087600 4087858 4087600 ID=Msa0831060-mRNA-1:exon:1638;Parent=Msa0831060-mRNA-1
chr5_4 five_prime_UTR 4084214 4084310 4084214 ID=Msa0831060-mRNA-1:five_prime_utr;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4084311 4084450 4084311 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4085564 4085612 4085564 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4085883 4086046 4085883 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4086138 4086175 4086138 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4086497 4086609 4086497 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4086727 4086840 4086727 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4086934 4087272 4086934 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 CDS 4087600 4087683 4087600 ID=Msa0831060-mRNA-1:cds;Parent=Msa0831060-mRNA-1
chr5_4 three_prime_UTR 4087684 4087858 4087684 ID=Msa0831060-mRNA-1:three_prime_utr;Parent=Msa0831060-mRNA-1
Gene Sequence

>Msa0831060

ATGGCGTCTCAAGATTCAGCTGGAAAGACAAATGATCAGTCTAAAGTCTCCGCAATCGCCGTTGAAGGTGCCAAACCTCCTAATCACGCTTTTCTCAGTATCTGCAAATCTCTTGTCGCAGGTGGAGTCGCCGGTGGAGTTTCACGAACAGCTGTGGCTCCTCTAGAGAGATTGAAGATTCTGCTGCAGGTTCAAAATCGTCACAATGTAAAATACAACGGAACAGTTCAGGGCTTAAAATATATATGGAAAACTGAGGGTTTTAGGGGAATGTTTAAAGGAAACGGAACCAATTGTGCTCGTATTATCCCAAATTCAGCAGTGAAGTTCTTTAGCTATGAGCAAGCATCTAATGGTATTCTAAGTCTCTATCGGTTGCAAACCGGAAATGAAGAAGCTCAACTCACTCCTCTTCTGCGTCTTGGAGGTGGGGCATGTGCAGGAATCATTGCAATGTCCGCAACTTACCCGATGGACCTGGTGCGAGGACGCCTAACTGTACAGACAGAAGCATCTCCTCATCAATACAGAGGAATTTTTAATGCTCTTTCAACAGTCTTCCGGGAAGAAGGCGCTAGAGCATTGTATAAAGGATGGTTACCTTCAGTCATAGGAGTTATACCGTACGTGGGTTTAAATTTTTCTGTATATGAATCTCTAAAAGACTGGTTGATTAAAACAAAACCACTTGGAATTGCTCAAGACTCAGAGCTAAGTGTGACCACAAGGCTTGCATGTGGGGCTGCTGCTGGAACTATTGGCCAGACAGTGGCATACCCCCTTGATGTCATTCGCAGAAGAATGCAGATGGGAGGCTGGAAAGGCGCTGCTTCCGTTGTCACTGGTGATGGGAAGGGTAATCTTGAGTACACTGGCATGGTTGATGCATTCAGTAAAACTGTAAAGTATGAGGGATTTGGAGCTTTATACAAGGGCTTAGTACCCAATTCAGTCAAGGTGGTCCCTTCCATAGCCATTGCCTTCGTGACATACGAGATGGTCAAGGACGTTCTAGGAGTTGAAATGAGAATATCTGACTGA
Protein sequence

>Msa0831060

MASQDSAGKTNDQSKVSAIAVEGAKPPNHAFLSICKSLVAGGVAGGVSRTAVAPLERLKILLQVQNRHNVKYNGTVQGLKYIWKTEGFRGMFKGNGTNCARIIPNSAVKFFSYEQASNGILSLYRLQTGNEEAQLTPLLRLGGGACAGIIAMSATYPMDLVRGRLTVQTEASPHQYRGIFNALSTVFREEGARALYKGWLPSVIGVIPYVGLNFSVYESLKDWLIKTKPLGIAQDSELSVTTRLACGAAAGTIGQTVAYPLDVIRRRMQMGGWKGAASVVTGDGKGNLEYTGMVDAFSKTVKYEGFGALYKGLVPNSVKVVPSIAIAFVTYEMVKDVLGVEMRISD*