AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0831220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0831220 MtrunA17_Chr5g0395061 95.131 267 7 1 1 267 1 261 0.0 532
Msa0831220 MtrunA17_Chr6g0468311 75.000 48 6 1 1 48 1 42 4.26e-18 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0831220 AT1G64980.2 78.277 267 55 2 3 267 12 277 4.69e-163 453
Msa0831220 AT1G64980.1 79.401 267 45 3 1 267 1 257 2.20e-162 451
Msa0831220 AT1G64980.3 79.401 267 45 3 1 267 1 257 2.20e-162 451

Find 90 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCCTTTCTTTCTTTC+TGG 0.032183 5_4:+4222083 Msa0831220:five_prime_UTR
CTCAGATCTGATTGCTTAAT+TGG 0.085095 5_4:-4222926 None:intergenic
TCCATCTTTGTAGTCTCTTT+TGG 0.193307 5_4:-4223154 None:intergenic
ATTGAAGACCAATTCTTTCT+AGG 0.195136 5_4:-4223199 None:intergenic
TCTCTATTTAGCTGATATTA+AGG 0.242104 5_4:+4223072 Msa0831220:CDS
ACGTGAAGACATTGCATTTC+AGG 0.259904 5_4:+4222853 Msa0831220:CDS
ATTTCCTTGAAGGACATAAT+AGG 0.275347 5_4:+4223356 Msa0831220:CDS
AAGATTCTTAACACCTAGTT+TGG 0.296665 5_4:+4223009 Msa0831220:CDS
TGTTTAGAGTCAGTCAATTA+TGG 0.301013 5_4:+4223577 Msa0831220:three_prime_UTR
TGGCTTGAGGATGATGAAAT+TGG 0.336305 5_4:+4223313 Msa0831220:CDS
GGAGTAAGAACCTTGTTCTT+AGG 0.346332 5_4:-4223244 None:intergenic
TTTAGCTGATATTAAGGAAT+TGG 0.346940 5_4:+4223078 Msa0831220:CDS
TAGAGATTTGAAGATGCATT+TGG 0.347816 5_4:+4223535 Msa0831220:exon
TTGCGATTATGCATTGTTCT+TGG 0.347846 5_4:+4223822 Msa0831220:three_prime_UTR
CTTTCCTTCATAGGTTTCAA+TGG 0.348106 5_4:+4223293 Msa0831220:CDS
GAGGGCCGTGGTTTGAGGCT+TGG 0.352682 5_4:+4223431 Msa0831220:CDS
AGACTGGTGCTTTCCTTCAT+AGG 0.356440 5_4:+4223284 Msa0831220:CDS
TCGGGGAGGGCCGTGGTTTG+AGG 0.366626 5_4:+4223426 Msa0831220:CDS
ATACTGTATCGAGTAAATCT+AGG 0.369673 5_4:-4221932 None:intergenic
ACGGCCCTCCCCGAGTATAA+TGG 0.377630 5_4:-4223417 None:intergenic
TTATTCTTAGACCTCTTGTC+AGG 0.383421 5_4:-4223762 None:intergenic
ATTGCGAATTTGCTGATCTA+TGG 0.387264 5_4:+4223458 Msa0831220:CDS
TTTCCTTGAAGGACATAATA+GGG 0.389168 5_4:+4223357 Msa0831220:CDS
TACTAGTTGGTAATGGTGAT+TGG 0.390769 5_4:-4222757 None:intergenic
TAGAAAGAATTGGTCTTCAA+TGG 0.393256 5_4:+4223201 Msa0831220:CDS
CAGCCCTATTATGTCCTTCA+AGG 0.399988 5_4:-4223360 None:intergenic
TTAATTGGTATGATCTCAAC+AGG 0.402966 5_4:-4222911 None:intergenic
TCAAGGTTGGGCTATGTTTG+TGG 0.418527 5_4:+4223039 Msa0831220:CDS
ATTTGTTTAATGACCTATGT+AGG 0.427955 5_4:+4223738 Msa0831220:three_prime_UTR
CCTAGTTTGGCAAATTATCA+AGG 0.430049 5_4:+4223022 Msa0831220:CDS
CCTTGATAATTTGCCAAACT+AGG 0.430154 5_4:-4223022 None:intergenic
ATATTCTATAATCCAGCTAT+TGG 0.437101 5_4:+4221853 Msa0831220:five_prime_UTR
TTTGGCAAATTATCAAGGTT+GGG 0.439482 5_4:+4223027 Msa0831220:CDS
AAACCCTTCAAGATCTTTGT+GGG 0.439682 5_4:+4222821 Msa0831220:CDS
CAAGTGTTGTTGGACTTGTT+TGG 0.442730 5_4:-4222060 None:intergenic
GTCTGTGAATTCACAGCATC+AGG 0.443576 5_4:-4223265 None:intergenic
CTGTGTATCCTAGAAAGAAT+TGG 0.452193 5_4:+4223191 Msa0831220:CDS
TTTGTTTGGAATTTCCTTGA+AGG 0.455406 5_4:+4223346 Msa0831220:CDS
CGAGTATAATGGATAGCCTT+AGG 0.455563 5_4:-4223406 None:intergenic
GTTTGGCAAATTATCAAGGT+TGG 0.457948 5_4:+4223026 Msa0831220:CDS
AAGGAGGGGACAAGTGTTGT+TGG 0.460012 5_4:-4222070 None:intergenic
AGCTTCAATATAGTTTGTGC+AGG 0.461318 5_4:+4223867 Msa0831220:three_prime_UTR
GAAACCCTTCAAGATCTTTG+TGG 0.462717 5_4:+4222820 Msa0831220:CDS
ATGATGCAAGTTATGAGAAT+AGG 0.469528 5_4:+4223651 Msa0831220:three_prime_UTR
ATTCCATTACCATTACTAGT+TGG 0.470434 5_4:-4222770 None:intergenic
TCAAGGAAATTCCAAACAAA+AGG 0.473734 5_4:-4223343 None:intergenic
GAAGAGAAATAGAGAAAGAG+AGG 0.476636 5_4:-4222019 None:intergenic
ATTATACTCGGGGAGGGCCG+TGG 0.478358 5_4:+4223419 Msa0831220:CDS
CTAAGGCTATCCATTATACT+CGG 0.478464 5_4:+4223407 Msa0831220:CDS
GTCGTCATTCAATCATGAAA+AGG 0.493012 5_4:+4222879 Msa0831220:CDS
TTACCATTACTAGTTGGTAA+TGG 0.495217 5_4:-4222764 None:intergenic
TATATTGCAATACCAATAGC+TGG 0.497053 5_4:-4221865 None:intergenic
TCCAAAAGAGACTACAAAGA+TGG 0.508455 5_4:+4223153 Msa0831220:CDS
GGAATTGGTAGATTTGATTG+AGG 0.511326 5_4:+4223093 Msa0831220:CDS
TTACCAACTAGTAATGGTAA+TGG 0.521531 5_4:+4222767 Msa0831220:CDS
GGTCTTTATTGGCGTGAAAG+AGG 0.522858 5_4:+4222956 Msa0831220:CDS
TAATTACCAGAAAGAAAGAA+AGG 0.528833 5_4:-4222089 None:intergenic
TACCAGAAAGAAAGAAAGGA+GGG 0.529239 5_4:-4222085 None:intergenic
CAAGCCATTGAAACCTATGA+AGG 0.539155 5_4:-4223297 None:intergenic
ATAACCCACAAAGATCTTGA+AGG 0.541294 5_4:-4222825 None:intergenic
TACTGTATCGAGTAAATCTA+GGG 0.549887 5_4:-4221931 None:intergenic
TCATAGGTTTCAATGGCTTG+AGG 0.551220 5_4:+4223300 Msa0831220:CDS
CCTCTTGTCAGGGCCTACAT+AGG 0.556427 5_4:-4223751 None:intergenic
AAAATCAAATACATCATGAA+AGG 0.558701 5_4:-4223713 None:intergenic
TTCTTCCAAGCCTCAAACCA+CGG 0.559243 5_4:-4223436 None:intergenic
TAGAGAAAGAGAGGATTGAG+CGG 0.561034 5_4:-4222010 None:intergenic
TAAGGCTATCCATTATACTC+GGG 0.565710 5_4:+4223408 Msa0831220:CDS
TGATCTATGGCTGAATGAGA+TGG 0.566426 5_4:+4223471 Msa0831220:CDS
TAACCCACAAAGATCTTGAA+GGG 0.577120 5_4:-4222824 None:intergenic
CTGTGGTCATCCTAAGAACA+AGG 0.579621 5_4:+4223234 Msa0831220:CDS
TATTCTTAGACCTCTTGTCA+GGG 0.580570 5_4:-4223761 None:intergenic
TGATCCAACTACTTCACCTA+AGG 0.585896 5_4:+4223390 Msa0831220:CDS
TCACCATTACCAACTAGTAA+TGG 0.590252 5_4:+4222761 Msa0831220:CDS
ATAGCCTTAGGTGAAGTAGT+TGG 0.594477 5_4:-4223394 None:intergenic
TATAACCCTCCCTCCACTGT+CGG 0.604729 5_4:+4221972 Msa0831220:five_prime_UTR
CCTATGTAGGCCCTGACAAG+AGG 0.608691 5_4:+4223751 Msa0831220:three_prime_UTR
ACATGTTCACCGACAGTGGA+GGG 0.612323 5_4:-4221981 None:intergenic
ACGCACATGTTCACCGACAG+TGG 0.617473 5_4:-4221985 None:intergenic
CACATGTTCACCGACAGTGG+AGG 0.623716 5_4:-4221982 None:intergenic
GATGCTGTGAATTCACAGAC+TGG 0.623826 5_4:+4223268 Msa0831220:CDS
AAAGAGACTACAAAGATGGA+TGG 0.623848 5_4:+4223157 Msa0831220:CDS
GTTCACCGACAGTGGAGGGA+GGG 0.634569 5_4:-4221977 None:intergenic
TTACCAGAAAGAAAGAAAGG+AGG 0.643019 5_4:-4222086 None:intergenic
GCTATCCATTATACTCGGGG+AGG 0.652245 5_4:+4223412 Msa0831220:CDS
TGTTCACCGACAGTGGAGGG+AGG 0.653233 5_4:-4221978 None:intergenic
ACCAGAAAGAAAGAAAGGAG+GGG 0.656464 5_4:-4222084 None:intergenic
ACAGAGATGAGTTTGAGCAA+TGG 0.659058 5_4:+4222728 Msa0831220:intron
CTATCCATTATACTCGGGGA+GGG 0.668674 5_4:+4223413 Msa0831220:CDS
AAGGCTATCCATTATACTCG+GGG 0.680190 5_4:+4223409 Msa0831220:CDS
TGAAATGCAATGTCTTCACG+TGG 0.692922 5_4:-4222851 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_4:4222472-4222491 Msa0831220:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_4:4222473-4222492 Msa0831220:intron 10.0%
!!! TTTTTTTTTATATGATCAAA+TGG + chr5_4:4222472-4222491 Msa0831220:intron 10.0%
!!! TTTTTTTTATATGATCAAAT+GGG + chr5_4:4222473-4222492 Msa0831220:intron 10.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_4:4222266-4222285 Msa0831220:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_4:4222306-4222325 Msa0831220:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_4:4222367-4222386 Msa0831220:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_4:4222407-4222426 Msa0831220:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_4:4222408-4222427 Msa0831220:intron 15.0%
!! GAAAAAAAAACAACAAATTA+AGG - chr5_4:4222706-4222725 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_4:4223515-4223534 None:intergenic 15.0%
!!! TTTTTTGTATATGATCAAAT+GGG + chr5_4:4222266-4222285 Msa0831220:intron 15.0%
!! TTTGAATTATTTCAACAATT+GGG + chr5_4:4222306-4222325 Msa0831220:intron 15.0%
!!! TTTTTTCTATATGATCAAAT+GGG + chr5_4:4222367-4222386 Msa0831220:intron 15.0%
!! ATTTGAATTATTACAACAAT+TGG + chr5_4:4222407-4222426 Msa0831220:intron 15.0%
!! TTTGAATTATTACAACAATT+GGG + chr5_4:4222408-4222427 Msa0831220:intron 15.0%
!! GAAAAAAAAACAACAAATTA+AGG - chr5_4:4222706-4222725 None:intergenic 15.0%
!!! ATTATCAGATTTTTCTTTTT+TGG - chr5_4:4223515-4223534 None:intergenic 15.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_4:4222265-4222284 Msa0831220:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_4:4222305-4222324 Msa0831220:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_4:4222366-4222385 Msa0831220:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_4:4222583-4222602 Msa0831220:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_4:4223716-4223735 None:intergenic 20.0%
!!! GTTTTTTGTATATGATCAAA+TGG + chr5_4:4222265-4222284 Msa0831220:intron 20.0%
!! CTTTGAATTATTTCAACAAT+TGG + chr5_4:4222305-4222324 Msa0831220:intron 20.0%
!!! GTTTTTTCTATATGATCAAA+TGG + chr5_4:4222366-4222385 Msa0831220:intron 20.0%
!! TATCACATTATTAAAACAGT+TGG + chr5_4:4222583-4222602 Msa0831220:intron 20.0%
!! AAAATCAAATACATCATGAA+AGG - chr5_4:4223716-4223735 None:intergenic 20.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_4:4221853-4221872 Msa0831220:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_4:4222092-4222111 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_4:4222204-4222223 Msa0831220:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_4:4222205-4222224 Msa0831220:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_4:4222315-4222334 Msa0831220:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_4:4222531-4222550 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_4:4222646-4222665 Msa0831220:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_4:4222647-4222666 Msa0831220:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_4:4223072-4223091 Msa0831220:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_4:4223078-4223097 Msa0831220:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_4:4223738-4223757 Msa0831220:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_4:4223791-4223810 Msa0831220:three_prime_UTR 25.0%
! ATATTCTATAATCCAGCTAT+TGG + chr5_4:4221853-4221872 Msa0831220:five_prime_UTR 25.0%
! TAATTACCAGAAAGAAAGAA+AGG - chr5_4:4222092-4222111 None:intergenic 25.0%
! TATCACATTATTACAACAAC+TGG + chr5_4:4222204-4222223 Msa0831220:intron 25.0%
! ATCACATTATTACAACAACT+GGG + chr5_4:4222205-4222224 Msa0831220:intron 25.0%
!! TTTCAACAATTGGGTTATTT+TGG + chr5_4:4222315-4222334 Msa0831220:intron 25.0%
!! TCTCTAAAAGAACAAATCAA+TGG - chr5_4:4222531-4222550 None:intergenic 25.0%
!!! TTTTTTCTGTCATGATCAAA+TGG + chr5_4:4222646-4222665 Msa0831220:intron 25.0%
!! TTTTTCTGTCATGATCAAAT+GGG + chr5_4:4222647-4222666 Msa0831220:intron 25.0%
! TCTCTATTTAGCTGATATTA+AGG + chr5_4:4223072-4223091 Msa0831220:CDS 25.0%
! TTTAGCTGATATTAAGGAAT+TGG + chr5_4:4223078-4223097 Msa0831220:CDS 25.0%
! ATTTGTTTAATGACCTATGT+AGG + chr5_4:4223738-4223757 Msa0831220:three_prime_UTR 25.0%
!!! ATTGAGTGTTTTAATCAACA+AGG + chr5_4:4223791-4223810 Msa0831220:three_prime_UTR 25.0%
TATATTGCAATACCAATAGC+TGG - chr5_4:4221868-4221887 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_4:4221934-4221953 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_4:4221935-4221954 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_4:4222121-4222140 Msa0831220:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_4:4222767-4222786 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_4:4222767-4222786 Msa0831220:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_4:4222773-4222792 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_4:4222914-4222933 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_4:4222945-4222964 Msa0831220:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_4:4223009-4223028 Msa0831220:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_4:4223027-4223046 Msa0831220:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_4:4223202-4223221 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_4:4223201-4223220 Msa0831220:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_4:4223217-4223236 Msa0831220:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_4:4223346-4223365 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_4:4223346-4223365 Msa0831220:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_4:4223356-4223375 Msa0831220:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_4:4223357-4223376 Msa0831220:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_4:4223535-4223554 Msa0831220:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_4:4223577-4223596 Msa0831220:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_4:4223651-4223670 Msa0831220:three_prime_UTR 30.0%
TATATTGCAATACCAATAGC+TGG - chr5_4:4221868-4221887 None:intergenic 30.0%
TACTGTATCGAGTAAATCTA+GGG - chr5_4:4221934-4221953 None:intergenic 30.0%
ATACTGTATCGAGTAAATCT+AGG - chr5_4:4221935-4221954 None:intergenic 30.0%
CTTTCACATTTCATTCGATT+GGG + chr5_4:4222121-4222140 Msa0831220:intron 30.0%
!! TTACCATTACTAGTTGGTAA+TGG - chr5_4:4222767-4222786 None:intergenic 30.0%
TTACCAACTAGTAATGGTAA+TGG + chr5_4:4222767-4222786 Msa0831220:CDS 30.0%
ATTCCATTACCATTACTAGT+TGG - chr5_4:4222773-4222792 None:intergenic 30.0%
TTAATTGGTATGATCTCAAC+AGG - chr5_4:4222914-4222933 None:intergenic 30.0%
TGAGAAAAAGTGGTCTTTAT+TGG + chr5_4:4222945-4222964 Msa0831220:CDS 30.0%
AAGATTCTTAACACCTAGTT+TGG + chr5_4:4223009-4223028 Msa0831220:CDS 30.0%
TTTGGCAAATTATCAAGGTT+GGG + chr5_4:4223027-4223046 Msa0831220:CDS 30.0%
ATTGAAGACCAATTCTTTCT+AGG - chr5_4:4223202-4223221 None:intergenic 30.0%
!! TAGAAAGAATTGGTCTTCAA+TGG + chr5_4:4223201-4223220 Msa0831220:CDS 30.0%
!!! TCAATGGTTTTGTATAACTG+TGG + chr5_4:4223217-4223236 Msa0831220:CDS 30.0%
TCAAGGAAATTCCAAACAAA+AGG - chr5_4:4223346-4223365 None:intergenic 30.0%
! TTTGTTTGGAATTTCCTTGA+AGG + chr5_4:4223346-4223365 Msa0831220:CDS 30.0%
ATTTCCTTGAAGGACATAAT+AGG + chr5_4:4223356-4223375 Msa0831220:CDS 30.0%
TTTCCTTGAAGGACATAATA+GGG + chr5_4:4223357-4223376 Msa0831220:CDS 30.0%
TAGAGATTTGAAGATGCATT+TGG + chr5_4:4223535-4223554 Msa0831220:exon 30.0%
! TGTTTAGAGTCAGTCAATTA+TGG + chr5_4:4223577-4223596 Msa0831220:three_prime_UTR 30.0%
ATGATGCAAGTTATGAGAAT+AGG + chr5_4:4223651-4223670 Msa0831220:three_prime_UTR 30.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_4:4222022-4222041 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_4:4222088-4222107 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_4:4222089-4222108 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_4:4222123-4222142 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_4:4222120-4222139 Msa0831220:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_4:4222760-4222779 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_4:4222761-4222780 Msa0831220:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_4:4222821-4222840 Msa0831220:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_4:4222827-4222846 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_4:4222828-4222847 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_4:4222879-4222898 Msa0831220:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_4:4222929-4222948 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_4:4222935-4222954 Msa0831220:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_4:4223025-4223044 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_4:4223022-4223041 Msa0831220:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_4:4223026-4223045 Msa0831220:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_4:4223093-4223112 Msa0831220:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_4:4223153-4223172 Msa0831220:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_4:4223157-4223176 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_4:4223157-4223176 Msa0831220:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_4:4223191-4223210 Msa0831220:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_4:4223293-4223312 Msa0831220:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_4:4223332-4223351 Msa0831220:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_4:4223407-4223426 Msa0831220:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_4:4223408-4223427 Msa0831220:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_4:4223458-4223477 Msa0831220:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_4:4223504-4223523 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_4:4223764-4223783 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_4:4223765-4223784 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_4:4223822-4223841 Msa0831220:three_prime_UTR 35.0%
! AGCTTCAATATAGTTTGTGC+AGG + chr5_4:4223867-4223886 Msa0831220:three_prime_UTR 35.0%
! GCTTCAATATAGTTTGTGCA+GGG + chr5_4:4223868-4223887 Msa0831220:three_prime_UTR 35.0%
! CTTCAATATAGTTTGTGCAG+GGG + chr5_4:4223869-4223888 Msa0831220:three_prime_UTR 35.0%
GAAGAGAAATAGAGAAAGAG+AGG - chr5_4:4222022-4222041 None:intergenic 35.0%
TACCAGAAAGAAAGAAAGGA+GGG - chr5_4:4222088-4222107 None:intergenic 35.0%
TTACCAGAAAGAAAGAAAGG+AGG - chr5_4:4222089-4222108 None:intergenic 35.0%
CCAATCGAATGAAATGTGAA+AGG - chr5_4:4222123-4222142 None:intergenic 35.0%
CCTTTCACATTTCATTCGAT+TGG + chr5_4:4222120-4222139 Msa0831220:intron 35.0%
!! TACTAGTTGGTAATGGTGAT+TGG - chr5_4:4222760-4222779 None:intergenic 35.0%
TCACCATTACCAACTAGTAA+TGG + chr5_4:4222761-4222780 Msa0831220:CDS 35.0%
AAACCCTTCAAGATCTTTGT+GGG + chr5_4:4222821-4222840 Msa0831220:CDS 35.0%
TAACCCACAAAGATCTTGAA+GGG - chr5_4:4222827-4222846 None:intergenic 35.0%
ATAACCCACAAAGATCTTGA+AGG - chr5_4:4222828-4222847 None:intergenic 35.0%
GTCGTCATTCAATCATGAAA+AGG + chr5_4:4222879-4222898 Msa0831220:CDS 35.0%
CTCAGATCTGATTGCTTAAT+TGG - chr5_4:4222929-4222948 None:intergenic 35.0%
CAATCAGATCTGAGAAAAAG+TGG + chr5_4:4222935-4222954 Msa0831220:CDS 35.0%
! CCTTGATAATTTGCCAAACT+AGG - chr5_4:4223025-4223044 None:intergenic 35.0%
CCTAGTTTGGCAAATTATCA+AGG + chr5_4:4223022-4223041 Msa0831220:CDS 35.0%
GTTTGGCAAATTATCAAGGT+TGG + chr5_4:4223026-4223045 Msa0831220:CDS 35.0%
GGAATTGGTAGATTTGATTG+AGG + chr5_4:4223093-4223112 Msa0831220:CDS 35.0%
TCCAAAAGAGACTACAAAGA+TGG + chr5_4:4223153-4223172 Msa0831220:CDS 35.0%
! TCCATCTTTGTAGTCTCTTT+TGG - chr5_4:4223157-4223176 None:intergenic 35.0%
AAAGAGACTACAAAGATGGA+TGG + chr5_4:4223157-4223176 Msa0831220:CDS 35.0%
CTGTGTATCCTAGAAAGAAT+TGG + chr5_4:4223191-4223210 Msa0831220:CDS 35.0%
CTTTCCTTCATAGGTTTCAA+TGG + chr5_4:4223293-4223312 Msa0831220:CDS 35.0%
!!! TTGGTTCTGTACCTTTTGTT+TGG + chr5_4:4223332-4223351 Msa0831220:CDS 35.0%
CTAAGGCTATCCATTATACT+CGG + chr5_4:4223407-4223426 Msa0831220:CDS 35.0%
TAAGGCTATCCATTATACTC+GGG + chr5_4:4223408-4223427 Msa0831220:CDS 35.0%
!! ATTGCGAATTTGCTGATCTA+TGG + chr5_4:4223458-4223477 Msa0831220:CDS 35.0%
!!! TTTCTTTTTTGGCTTGAGCA+AGG - chr5_4:4223504-4223523 None:intergenic 35.0%
TATTCTTAGACCTCTTGTCA+GGG - chr5_4:4223764-4223783 None:intergenic 35.0%
TTATTCTTAGACCTCTTGTC+AGG - chr5_4:4223765-4223784 None:intergenic 35.0%
TTGCGATTATGCATTGTTCT+TGG + chr5_4:4223822-4223841 Msa0831220:three_prime_UTR 35.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_4:4222013-4222032 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG - chr5_4:4222052-4222071 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG - chr5_4:4222063-4222082 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_4:4222087-4222106 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_4:4222728-4222747 Msa0831220:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_4:4222820-4222839 Msa0831220:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_4:4222854-4222873 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_4:4222853-4222872 Msa0831220:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_4:4223247-4223266 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_4:4223300-4223319 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_4:4223300-4223319 Msa0831220:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_4:4223313-4223332 Msa0831220:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_4:4223390-4223409 Msa0831220:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_4:4223397-4223416 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_4:4223409-4223428 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_4:4223409-4223428 Msa0831220:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_4:4223471-4223490 Msa0831220:CDS 40.0%
TAGAGAAAGAGAGGATTGAG+CGG - chr5_4:4222013-4222032 None:intergenic 40.0%
! GGACTTGTTTGGAAAAAGAG+AGG - chr5_4:4222052-4222071 None:intergenic 40.0%
! CAAGTGTTGTTGGACTTGTT+TGG - chr5_4:4222063-4222082 None:intergenic 40.0%
ACCAGAAAGAAAGAAAGGAG+GGG - chr5_4:4222087-4222106 None:intergenic 40.0%
ACAGAGATGAGTTTGAGCAA+TGG + chr5_4:4222728-4222747 Msa0831220:intron 40.0%
GAAACCCTTCAAGATCTTTG+TGG + chr5_4:4222820-4222839 Msa0831220:CDS 40.0%
TGAAATGCAATGTCTTCACG+TGG - chr5_4:4222854-4222873 None:intergenic 40.0%
ACGTGAAGACATTGCATTTC+AGG + chr5_4:4222853-4222872 Msa0831220:CDS 40.0%
GGAGTAAGAACCTTGTTCTT+AGG - chr5_4:4223247-4223266 None:intergenic 40.0%
CAAGCCATTGAAACCTATGA+AGG - chr5_4:4223300-4223319 None:intergenic 40.0%
TCATAGGTTTCAATGGCTTG+AGG + chr5_4:4223300-4223319 Msa0831220:CDS 40.0%
TGGCTTGAGGATGATGAAAT+TGG + chr5_4:4223313-4223332 Msa0831220:CDS 40.0%
TGATCCAACTACTTCACCTA+AGG + chr5_4:4223390-4223409 Msa0831220:CDS 40.0%
! ATAGCCTTAGGTGAAGTAGT+TGG - chr5_4:4223397-4223416 None:intergenic 40.0%
!! CGAGTATAATGGATAGCCTT+AGG - chr5_4:4223409-4223428 None:intergenic 40.0%
AAGGCTATCCATTATACTCG+GGG + chr5_4:4223409-4223428 Msa0831220:CDS 40.0%
TGATCTATGGCTGAATGAGA+TGG + chr5_4:4223471-4223490 Msa0831220:CDS 40.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_4:4222083-4222102 Msa0831220:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_4:4222956-4222975 Msa0831220:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_4:4223039-4223058 Msa0831220:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_4:4223234-4223253 Msa0831220:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_4:4223268-4223287 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_4:4223268-4223287 Msa0831220:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_4:4223284-4223303 Msa0831220:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_4:4223363-4223382 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_4:4223413-4223432 Msa0831220:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_4:4223439-4223458 None:intergenic 45.0%
TCCCCTCCTTTCTTTCTTTC+TGG + chr5_4:4222083-4222102 Msa0831220:five_prime_UTR 45.0%
GGTCTTTATTGGCGTGAAAG+AGG + chr5_4:4222956-4222975 Msa0831220:CDS 45.0%
TCAAGGTTGGGCTATGTTTG+TGG + chr5_4:4223039-4223058 Msa0831220:CDS 45.0%
CTGTGGTCATCCTAAGAACA+AGG + chr5_4:4223234-4223253 Msa0831220:CDS 45.0%
GTCTGTGAATTCACAGCATC+AGG - chr5_4:4223268-4223287 None:intergenic 45.0%
GATGCTGTGAATTCACAGAC+TGG + chr5_4:4223268-4223287 Msa0831220:CDS 45.0%
AGACTGGTGCTTTCCTTCAT+AGG + chr5_4:4223284-4223303 Msa0831220:CDS 45.0%
CAGCCCTATTATGTCCTTCA+AGG - chr5_4:4223363-4223382 None:intergenic 45.0%
CTATCCATTATACTCGGGGA+GGG + chr5_4:4223413-4223432 Msa0831220:CDS 45.0%
TTCTTCCAAGCCTCAAACCA+CGG - chr5_4:4223439-4223458 None:intergenic 45.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_4:4221972-4221991 Msa0831220:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_4:4221984-4222003 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG - chr5_4:4222073-4222092 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_4:4223412-4223431 Msa0831220:CDS 50.0%
TATAACCCTCCCTCCACTGT+CGG + chr5_4:4221972-4221991 Msa0831220:five_prime_UTR 50.0%
ACATGTTCACCGACAGTGGA+GGG - chr5_4:4221984-4222003 None:intergenic 50.0%
! AAGGAGGGGACAAGTGTTGT+TGG - chr5_4:4222073-4222092 None:intergenic 50.0%
GCTATCCATTATACTCGGGG+AGG + chr5_4:4223412-4223431 Msa0831220:CDS 50.0%
CACATGTTCACCGACAGTGG+AGG - chr5_4:4221985-4222004 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_4:4221988-4222007 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_4:4223754-4223773 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_4:4223751-4223770 Msa0831220:three_prime_UTR 55.0%
CACATGTTCACCGACAGTGG+AGG - chr5_4:4221985-4222004 None:intergenic 55.0%
ACGCACATGTTCACCGACAG+TGG - chr5_4:4221988-4222007 None:intergenic 55.0%
CCTCTTGTCAGGGCCTACAT+AGG - chr5_4:4223754-4223773 None:intergenic 55.0%
CCTATGTAGGCCCTGACAAG+AGG + chr5_4:4223751-4223770 Msa0831220:three_prime_UTR 55.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_4:4221980-4221999 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_4:4221981-4222000 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_4:4223420-4223439 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_4:4223419-4223438 Msa0831220:CDS 60.0%
GTTCACCGACAGTGGAGGGA+GGG - chr5_4:4221980-4221999 None:intergenic 60.0%
TGTTCACCGACAGTGGAGGG+AGG - chr5_4:4221981-4222000 None:intergenic 60.0%
ACGGCCCTCCCCGAGTATAA+TGG - chr5_4:4223420-4223439 None:intergenic 60.0%
ATTATACTCGGGGAGGGCCG+TGG + chr5_4:4223419-4223438 Msa0831220:CDS 60.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_4:4223431-4223450 Msa0831220:CDS 65.0%
! GAGGGCCGTGGTTTGAGGCT+TGG + chr5_4:4223431-4223450 Msa0831220:CDS 65.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_4:4223426-4223445 Msa0831220:CDS 70.0%
TCGGGGAGGGCCGTGGTTTG+AGG + chr5_4:4223426-4223445 Msa0831220:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 4221841 4223889 4221841 ID=Msa0831220;Name=Msa0831220
chr5_4 mRNA 4221841 4223889 4221841 ID=Msa0831220-mRNA-1;Parent=Msa0831220;Name=Msa0831220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=264|0|0.5|1|0|0.5|2|352|267
chr5_4 exon 4221841 4222104 4221841 ID=Msa0831220-mRNA-1:exon:1699;Parent=Msa0831220-mRNA-1
chr5_4 exon 4222734 4223889 4222734 ID=Msa0831220-mRNA-1:exon:1700;Parent=Msa0831220-mRNA-1
chr5_4 five_prime_UTR 4221841 4222104 4221841 ID=Msa0831220-mRNA-1:five_prime_utr;Parent=Msa0831220-mRNA-1
chr5_4 CDS 4222734 4223537 4222734 ID=Msa0831220-mRNA-1:cds;Parent=Msa0831220-mRNA-1
chr5_4 three_prime_UTR 4223538 4223889 4223538 ID=Msa0831220-mRNA-1:three_prime_utr;Parent=Msa0831220-mRNA-1
Gene Sequence

>Msa0831220

ATGAGTTTGAGCAATGGTAAAAACCAATCACCATTACCAACTAGTAATGGTAATGGAATAGATTCTGCTGAGAATGAGAATACAAAGAAACCCTTCAAGATCTTTGTGGGTTATGATCCACGTGAAGACATTGCATTTCAGGTATGTCGTCATTCAATCATGAAAAGGTCTTCAATTCCTGTTGAGATCATACCAATTAAGCAATCAGATCTGAGAAAAAGTGGTCTTTATTGGCGTGAAAGAGGTCAATTTGAGAGCACTGAGTTTTCTTTTACAAGATTCTTAACACCTAGTTTGGCAAATTATCAAGGTTGGGCTATGTTTGTGGATTGTGATTTTCTCTATTTAGCTGATATTAAGGAATTGGTAGATTTGATTGAGGATAAGTATGCTATTATGTGTGTTCAACATGATTATACTCCAAAAGAGACTACAAAGATGGATGGTGCTGTTCAAACTGTGTATCCTAGAAAGAATTGGTCTTCAATGGTTTTGTATAACTGTGGTCATCCTAAGAACAAGGTTCTTACTCCTGATGCTGTGAATTCACAGACTGGTGCTTTCCTTCATAGGTTTCAATGGCTTGAGGATGATGAAATTGGTTCTGTACCTTTTGTTTGGAATTTCCTTGAAGGACATAATAGGGCTGTTGAGAATGATCCAACTACTTCACCTAAGGCTATCCATTATACTCGGGGAGGGCCGTGGTTTGAGGCTTGGAAGAATTGCGAATTTGCTGATCTATGGCTGAATGAGATGGAAGAGTACCTTGCTCAAGCCAAAAAAGAAAAATCTGATAATTAG
Protein sequence

>Msa0831220

MSLSNGKNQSPLPTSNGNGIDSAENENTKKPFKIFVGYDPREDIAFQVCRHSIMKRSSIPVEIIPIKQSDLRKSGLYWRERGQFESTEFSFTRFLTPSLANYQGWAMFVDCDFLYLADIKELVDLIEDKYAIMCVQHDYTPKETTKMDGAVQTVYPRKNWSSMVLYNCGHPKNKVLTPDAVNSQTGAFLHRFQWLEDDEIGSVPFVWNFLEGHNRAVENDPTTSPKAIHYTRGGPWFEAWKNCEFADLWLNEMEEYLAQAKKEKSDN*